Endress+Hauser analytikjena CyBio SELMA Series User manual

Operating Manual
CyBio SELMA
Semi-automatic pipetting station

Manufacturer Analytik Jena GmbH+Co. KG
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Technical Service Analytik Jena GmbH+Co. KG
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
General Information http://www.analytik-jena.com
Documentation Number OL7001-26-21XBLE
Edition C (06/2023)
Technical Documentation Analytik Jena GmbH+Co. KG
© Copyright 2023, Analytik Jena GmbH+Co. KG
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

CyBio SELMA Table of contents
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Table of contents
1 Basic information ................................................................................................................................................. 5
1.1 About this user manual .................................................................................................................................... 5
1.2 Intended use ..................................................................................................................................................... 5
1.3 Standards and directives .................................................................................................................................. 6
2 Safety.................................................................................................................................................................... 7
2.1 Safety labeling on the device ........................................................................................................................... 7
2.2 Danger zone...................................................................................................................................................... 8
2.3 Requirements for the operating personnel..................................................................................................... 9
2.4 Safety instructions for operation ..................................................................................................................... 9
2.5 Safety instructionsformaintenance and repair.............................................................................................. 10
2.5.1 Chemical resistance .......................................................................................................................................... 11
2.6 Behavior during emergencies .......................................................................................................................... 11
3 Technical description............................................................................................................................................ 12
3.1 Device configuration......................................................................................................................................... 12
3.2 Control elements .............................................................................................................................................. 13
3.3 Tray.................................................................................................................................................................... 15
3.4 External power supply unit .............................................................................................................................. 16
3.5 Connection ........................................................................................................................................................ 16
3.6 Device versions ................................................................................................................................................. 17
3.7 Scope of delivery............................................................................................................................................... 18
3.8 Function ............................................................................................................................................................ 18
3.9 The Pipetting Cycle........................................................................................................................................... 19
3.10 System precision ............................................................................................................................................... 23
4 Installation and commissioning ........................................................................................................................... 24
4.1 Installation conditions...................................................................................................................................... 24
4.1.1 Ambient conditions .......................................................................................................................................... 24
4.1.2 Power supply..................................................................................................................................................... 24
4.2 Commissioning ................................................................................................................................................. 25
4.3 Function Tests................................................................................................................................................... 26
4.3.1 Leak test............................................................................................................................................................ 27
4.3.2 Precision test..................................................................................................................................................... 28
4.3.3 Accuracy Test .................................................................................................................................................... 29
5 Operation.............................................................................................................................................................. 31
5.1 Operate the device via touchscreen................................................................................................................. 32
5.2 Priming.............................................................................................................................................................. 33
5.3 Pipetting............................................................................................................................................................ 35
5.4 Diluting.............................................................................................................................................................. 37
5.5 Dispensing......................................................................................................................................................... 39
5.6 Reverse pipetting.............................................................................................................................................. 42
5.7 Serial dilution.................................................................................................................................................... 45

Table of contents CyBio SELMA
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5.8 Advanced functions .......................................................................................................................................... 47
5.8.1 Tip replacement................................................................................................................................................ 47
5.8.2 Device settings.................................................................................................................................................. 48
5.8.3 Idle state............................................................................................................................................................ 49
5.8.4 Work with semi-automatic routines................................................................................................................ 49
6 Troubleshooting ................................................................................................................................................... 52
6.1 Entry of incorrect values................................................................................................................................... 52
6.2 Operation only with inserted tip holder .......................................................................................................... 52
6.3 Interrupt the pipetting routine with the STOP button ................................................................................... 52
6.4 Error handling................................................................................................................................................... 53
6.5 Power failure..................................................................................................................................................... 54
7 Maintenance and care.......................................................................................................................................... 55
7.1 Maintenance overview ..................................................................................................................................... 55
7.2 Service the plunger sealing system.................................................................................................................. 56
8 Transport and storage.......................................................................................................................................... 58
8.1 Transport........................................................................................................................................................... 58
8.2 Preparing the device for transport................................................................................................................... 58
8.3 Moving the device in the laboratory................................................................................................................ 59
8.4 Storage .............................................................................................................................................................. 60
9 Specifications........................................................................................................................................................ 61
10 Disposal ................................................................................................................................................................ 63

CyBio SELMA Basic information
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1 Basic information
1.1 About this user manual
Content The operating manual describes the semi-automatic pipetting station CyBio SELMA.
The device is intended to be operated by qualified specialist personnel observing this
user manual.
The manual provides information about the design and operation of the device and pro-
vides operating personnel with the necessary know-how for safe handling of the device
and its components. Furthermore, the manual includes information on the maintenance
and care of the device as well as indications for potential causes for malfunctions and
their correction.
The original manual is written in German. Editions in other languages are translated
from the original manual.
Conventions Instructions for actions occurring in chronological order are numbered and combined
into action units.
Warnings are indicated by a warning triangle and a signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the System menu).
¡Menu items are separated by vertical lines (e.g., System|Device).
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which can cause death or very serious (possi-
bly permanent) injury.
CAUTION
Indicates a potentially hazardous situation which can cause slight or minor injuries.
NOTICE
Provides information on potential material or environmental damage.
1.2 Intended use
The pipetting station CyBio SELMA has been developed for manual and semi-automatic
processing of microplates in chemical and biological laboratories. In the fields of
medicine and diagnostics, its use is limited to research.

Basic information CyBio SELMA
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The basic functions include the absorption and dispensing of liquid from and into reser-
voirs, microplates, and their individual columns. The device is suitable to be operated by
one person.
You can equip the device exclusively with the following tip holders:
¡Tip magazines (metal tip holders for long-term use)
¡CyBio TipTray (disposable tip holder made of plastic specially designed for CyBio
SELMA)
For proper use, please observe the following:
¡The device must only be operated by qualified and trained personnel.
¡The device must only be used in accordance with this manual. This applies in partic-
ular to the adherence to the connection values, conditions of use and notes on the
maintenance, transport, and disposal.
¡The safety instructions in this manual must be observed.
It is not permissible
¡to operate this equipment in a medical laboratory,
¡to work with explosive substances in this device,
¡to operate this device in an explosive environment.
¡to smoke or use a naked flame at the installation location.
As regards the safe handling of dangerous substances (radioactive, infectious, toxic, cor-
rosive, combustible, and other hazardous substances), the owner/operator will be re-
sponsible in accordance with applicable laws and guidelines.
The same applies in terms of compliance with environmental protection rules (e. g. for
disposal of reagents and consumables).
1.3 Standards and directives
The device was manufactured according to the currently valid technology regulations
and the approved safety related regulations. When constructing the device, the relevant
safety and health requirements of the applicable laws, standards and regulations were
applied and confirmed by the CE marking and declaration of conformity.
All information regarding safety correspond to the currently valid regulations of the Eu-
ropean Union. In other countries the applicable laws and country specific regulations
have to be complied with.

CyBio SELMA Safety
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2 Safety
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Observe all safety instructions listed in this manual, as well as all messages and instruc-
tions displayed by the control- and analysis software on the monitor.
2.1 Safety labeling on the device
Warning and mandatory action labels have been attached to the device and must always
be observed.
Damaged or missing warning and mandatory action labels can cause incorrect actions
leading to personal injury or material damage. The labels must not be removed. Dam-
aged warning and mandatory action labels must be replaced immediately!
The following warning and mandatory action labels have been attached to the device:
Fig.1 Safety labeling on the device
Warning sym-
bol
Meaning Comment
Warning of biohazard Warning of hazardous biological and chemical
substances

Safety CyBio SELMA
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Warning sym-
bol
Meaning Comment
Warning of a danger
point
Warning of mechanical hazard from moving
equipment parts.
Warning of crushing Warning of crushing in the area of the tip
mounting
2.2 Danger zone
2
3
5
1
4
Fig.2 Hazard area
1 STOP button 2 Movement area of the pipetting head
3 Tip mounting 4 Tray with two working positions
5 Rotary knob
The movement of the pipetting head can endanger the operating personnel. Failure to
observe warning information can result in crushing injuries to hands.
Any intervention into the movement area of the device during operation may cause ma-
terial damage to the device or the samples.
¡Never reach into the movement range of the pipetting head physically or with an
object during operation.

CyBio SELMA Safety
9
¡Immediately release the rotary knob in the case of maloperation (manual opera-
tion). The rotary knob will return to its mid-range position. Turn the knob in clock-
wise direction in order to restore the pipetting head to zero position.
¡To abort a program, press the STOP button (semi-automatic operation).
¡Always correct any faulty movements on the touchscreen of the device. Faulty ac-
tions or maloperations may cause material damage or physical injury otherwise.
¡Do not move the pipetting head against the tray.
2.3 Requirements for the operating personnel
The device may only be operated by trained specialist personnel instructed in technical
safety. The operating personnel must have read and understood the operating instruc-
tions.
The personal protective equipment must be worn to operate or service the device.
The operating personnel must be familiar with the dangers arising from the substances
used.
2.4 Safety instructions for operation
Handling hazardous substances The operator is responsible for the selection of substances used in the process as well as
for their safe handling. This is particularly important for radioactive, infectious, poi-
sonous, corrosive, combustible, explosive and otherwise dangerous substances.
When handling hazardous substances, the locally applicable safety instructions and in-
structions in the safety data sheets from the manufacturers of the auxiliary and operat-
ing materials must be complied with.
Observe the following:
¡The operator is responsible for carrying out suitable decontamination should the de-
vice become contaminated externally or internally with dangerous substances.
¡Splashes, drops or larger liquid spillages should be removed using an absorbent ma-
terial such as cotton wool, laboratory wipes or cellulose.
¡For biological contamination, wipe the affected area with a suitable disinfectant,
such as an Incidin Plus solution. Then wipe the cleaned areas so that they are dry.
¡The only suitable cleaning method for the housing is wipe disinfection. If the disin-
fectant has a spray nozzle, apply disinfectant to a suitable cloth before using it on
the device.
Work particularly carefully and cleanly with infectious material because the device
cannot be decontaminated as a whole.
¡Before using a cleaning or decontamination procedure other than that prescribed by
the manufacturer, the user is required to check with the manufacturer that the in-
tended procedure will not damage the device. Safety labels attached to the device
must not have methanol applied.
Start-up and safe operation The operator must make sure that the device and its safety equipment is in sound condi-
tion each time before starting up the device. This applies in particular after each modifi-
cation or extension of the device or its repair.
Observe the following:
¡The device may only be operated if all items of protective equipment (e.g. covers in
front of electronic components) are in place, properly installed and fully operational.

Safety CyBio SELMA
10
¡The sound condition of the protection and safety equipment must be checked regu-
larly. Any defects must be corrected as soon as they occur.
¡Protective and safety equipment must never be removed, modified or switched off
during operation.
¡Always ensure free access to the main switch and to the emergency shutdown
switches and locks during operation.
¡The external power supply unit must be connected to a proper power outlet to en-
sure that the device (ground connector) meets the protection class I. Do not replace
the removable power supply unit with a different power supply unit (without protec-
tive ground conductor). Extensions of the supply cable are not permitted!
¡Keep all combustible materials away from the device.
¡The ventilation equipment on the device must be in good working condition. Cov-
ered ventilation grilles or slots etc. may cause the device to break down or may
cause damage to it.
¡Ensure that no liquid enters the interior of the device, for example at cable connec-
tions. There is a danger of electric shock.
¡Modifications, conversions and extensions to the device are only permitted after
consultation with Analytik Jena. Unauthorized modifications can jeopardize the de-
vice’s operational safety and may lead to limitations regarding the warranty and ac-
cess to customer service.
2.5 Safety instructionsformaintenance and repair
The device is generally maintained by the customer service department of Analytik Jena
or specialist personnel trained and authorized by them.
Unauthorized maintenance can damage the device. For this reason, only the activities
described in the user manual in the "Maintenance and care" chapter may be performed
by the operator.
¡Work on the electronics may only be carried out by the customer service of Analytik
Jena and specially authorized technicians.
¡All maintenance and repair work on the device must only be carried out when the
device is switched off (unless specified otherwise).
¡Touching live parts can result in death, injury, or damage to property. Always unplug
the power cord from the power outlet first. Only then disconnect the power cord
from the power supply unit. Never open the power supply unit and do not carry out
any repairs on the power supply unit. If any error occurs, contact customer service.
¡Only clean the exterior of the device with a slightly moistened, non-dripping cloth.
Use only water and, if required, customary surfactants.
¡Do not use organic solvents or abrasives to clean the device. Exercise caution when
decontaminating the device with disinfectants containing alcohol. The alcohol can
damage the safety labeling on the device.
¡Use only original spare parts, wear parts and consumables. They have been tested
and ensure safe operation. Glass part are wear parts and are not subject to the war-
ranty.
¡All protective equipment must be reinstalled and checked for proper function when
the maintenance or repair work is complete.
¡Risk of damage to health due to improper decontamination! Perform a professional
and documented decontamination of the device before returning it to Analytik Jena.
The decontamination report is available from Service when registering the return.
Without a completed decontamination report, the acceptance of the device will be
refused. The sender may be liable for damage caused by inadequate decontamina-
tion of the device.

CyBio SELMA Safety
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2.5.1 Chemical resistance
Aggressive substances may damage the device. Although the materials used are resis-
tant to most of the commonly used substances, material damage from aggressive sub-
stances cannot be completely excluded.
¡Before using any aggressive substances, e.g., bases, acids, or organic solutions:
Check whether the materials are resistant to this.
¡Only use substances compatible with the materials listed.
¡When in doubt, consult the manufacturer.
Component Material
Pipette tips PP
¡Plunger (contact via aerosols is possible)
¡Plunger seals
¡Stainless steel
¡PE-HD
Reagent cups PMMA; PTFE
Examples of aggressive substances and mixtures of substances against which there is in-
sufficient resistance:
¡Hydrofluoric acid (HF/hydrofluoric acid)
¡Highly concentrated acids
¡naphtha
¡Gasoline
¡Acetone
¡Ozone
¡Oxidizing solutions
¡Cleaning spray
¡Cleaning powder
¡Paint thinner
2.6 Behavior during emergencies
If there is no immediate risk of injury, stop the device movement in a dangerous situa-
tion with the STOP button.
Alternatively, turn the device off using the on/off switch and/or pull the power plug out
of the power outlet.

Technical description CyBio SELMA
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3 Technical description
3.1 Device configuration
The semi-automatic pipetting station CyBio SELMA is used for the quick and precise pro-
cessing of microplates with 96 or 384 wells in the ANSI/SLAS standard. The device has
a pipetting head with 96 or 384 plungers that work simultaneously, with which it aspi-
rates and dispenses liquids via the proven tip sealing system. With the pipetting station
you can replicate or reformat microplates and make serial dilutions. Here, you will
achieve error-free and reproducible results.
You can equip the device with reusable metal tip magazines or ready-made plastic tip
holders (CyBio TipTray). The plastic tip holders are made for single use and can be
changed particularly quickly and easily.
The memory function enables fast processing of semi-automatic routines.
1
4
5
3
2
6
7
8
9
10
11
Fig.3 Device configuration
1 Light element 2 Guide groove I + II
3 Light element 4 Pipetting push button “pip”
5 Moving and pipetting push button
“move + pip”
6 Foldable touchscreen
7 STOP button 8 Pipetting head
9 TipTray 10 Tray with working positions I + II
11 Rotary knob

CyBio SELMA Technical description
13
The device is mainly operated via the touchscreen, which can be folded upwards. There-
fore, you can work sitting or standing. Two buttons on the support foot of the device al-
low you to choose between "pipetting" ("pip" button) and "pipetting with saved values"
("move + pip" button). Semi-automatic work processes can be interrupted with the stop
button. You can use the rotary knob to move the pipetting head to pipette over the mi-
croplates.
Glowing light elements indicate the switched-on state (when activated).
You can process microplates with 96 wells or 384 wells in a single step. For models with
96 channels, you can also use an adapter to position microplates with 384 wells in the
XY direction on a tray and process them in four steps.
3.2 Control elements
On/off switch and standby The device has an on/off switch on the back of the device.
2
4
5
3
1
Fig.4 Device rear
1 Type plate 2 Cover flaps or connectors
3 On/off switch (O/I) 4 Service interface
5 DC input
You can activate the standby mode in the device settings. The device then goes into idle
state after the specified time. The standard value is at 30min.
In standby mode, the power consumption is reduced and the touchscreen lighting is
switched off. If you touch the touchscreen or operate an operating element on the sup-
port foot of the device, the device is activated again.
STOP button Use the STOP button to interrupt a running pipetting process. The device immediately
stops all movements of the plunger and pipetting head. The pipetting head stands still.
You can continue or abort the pipetting process via the touchscreen. You can move the
pipetting head upwards with the rotary knob.
Push button "pip" Trigger the movement of the plungers in the pipetting head with the "pip" button on the
support foot of the device. The device then automatically carries out the plunger move-
ments and works through the following steps:
¡Aspirating a fluid
¡Dispensing the fluid
¡Residual ejection of the liquid (Blowout)
¡Move the plunger to the zero position

Technical description CyBio SELMA
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The pipetting head is not moved here, only the plungers.
Push button "move + pip" You can run pipetting routines repeatedly using the "move + pip" button.
In contrast to the "move + pip" button, the "pip" button also triggers the vertical move-
ment of the pipetting head. After pipetting, the pipetting head returns to its initial posi-
tion again.
The process is as follows:
¡Approaching the saved pipetting height
¡Pipetting
¡Approaching the height before pipetting
Rotary knob You can move the pipetting head in a vertical direction with the rotary knob on the right
support foot of the device. You can adjust the height of the pipetting head so that you
can change microplates. You can also use the rotary knob to position the pipette tips in
the well of a microplate.
The direction of travel of the pipetting head is determined by the direction of rotation.
Fig.5 Direction of rotation and travel
1 Turn to the left - pipetting head moves
downwards
2 Turn to the right - pipetting head moves
upwards
Deflecting the rotary knob changes the speed of the pipetting head. If the rotary knob is
in the idle position, the pipetting head will hold its current position. The greater the de-
flection, the faster the pipetting head moves.
When processing the pipetting routines, sometimes the pipette tips must be positioned
in the wells with an accuracy of up to 0.1 mm. Therefore, the speed of the pipetting
head does not increase linearly with the deflection of the rotary knob.
The following values can be assumed as benchmarks:
¡Mean response time (awareness to motor-driven implementation): approx.
0.5to1s
¡Minimum resolution of the movement: approx. 0.1mm
Touchscreen You operate the device mainly via the touchscreen. The touchscreen can be swiveled up-
wards and can therefore be operated easily while sitting and standing.
First, set all values for the pipetting process via the touchscreen. You can then trigger
the pipetting process using one of the following buttons or the buttons on the touch-
screen:
¡"pip" button on the support foot of the device
¡"move + pip" button on the support foot of the device
¡Button on the touchscreen
You can continue the pipetting process after a step of manual operation with one of the
two buttons or the corresponding button on the touchscreen. Operation via the touch-
screen is easier, especially when standing.

CyBio SELMA Technical description
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Menu H: -0.0mm
Priming Pipetting
Sample Dilution Repeat Dispensing
Reverse Pipetting
Serial Dilution
Device Settings Change Tips
12
3
Fig.6 Button layout
1 Information about the current pipetting
step in the menu
2 Current height of the pipetting head
3 Working area with various control ele-
ments and information
3.3 Tray
The tray has two working positions. Working position 1 is fixed. Working position 2 can
be shifted to the left entirely as well as column by column in grids. Thus the device can
process individual columns of a microplate without any problems.
1
2
3
4
Fig.7 Tray with working position 1 and 2
1 Working position 2 (can be shifted en-
tirely and column by column)
2 Working position 1 (fixed)
3 Spring lever for selecting the individual
columns
4 Column scale
The tray can also be inserted into the device in two guide grooves (I or II). This means
that the device can also transfer liquid from reservoirs with deep well pipette tips into
deep well microplates easily.

Technical description CyBio SELMA
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Fig.8 Guide groove I and II
3.4 External power supply unit
The power supply unit is a wide range power supply unit. The power is supplied on the
primary side at a power outlet with 100to240V ±10% (1.7A max); 50/60Hz. On the
secondary side, the power supply unit provides a voltage of 24V (2.5A max.).
Fig.9 External power supply unit
3.5 Connection
The connections, the on/off switch, and the type plate are located on the back of the de-
vice.

CyBio SELMA Technical description
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2
4
5
3
1
Fig.10 Device rear
1 Type plate 2 Cover flaps or connectors
3 On/off switch (O/I) 4 Service interface
5 DC input
Depending on the device version, two cover caps or a cover flap and a connector are at-
tached to the rear side. Never remove these parts.
The type plate contains the following information:
¡company name and complete address of the manufacturer
¡Name of the machine (type designation and trade name)
¡model and serial number
¡Year of manufacture
¡Electrical connection data
¡CE mark and WEEE disposal mark
3.6 Device versions
The device type is noted on the front of the pipetting head.
Device type Number of pipette
tips
Maximum volume
for each plunger
Can be dosed in
steps of
CyBio SELMA
96/25µl
96 25µl 0.01µl
CyBio SELMA
96/60µl
96 60µl 0.01µl
CyBio SELMA
96/250µl
96 250µl 0.1µl
CyBio SELMA
96/1000µl
96 1000µl 0.1µl
CyBio SELMA
384/25µl
384 25µl 0.01µl
CyBio SELMA
384/60µl
384 60µl 0.01µl

Technical description CyBio SELMA
18
3.7 Scope of delivery
The scope of delivery includes:
¡Pipetting station with moving tray
¡Power supply unit
¡Country-specific power cord
¡Box with CyBio TipTray (in standard quality)
¡Operating instructions
¡Optional accessories
3.8 Function
With the pipetting station CyBio SELMA, you can process microplates semi-automati-
cally in the ANSI/SLAS standard. The device can work in the following pipetting modes.
As an added functionality, the device can automatically loosen and tighten the tip hold-
ers airtight again.
Pipetting modes /
basic functions
Explanation
Priming ¡Absorption of a defined volume
¡Flushing the pipette tips by repeatedly dispensing and aspirat-
ing the volume (source plate = target plate)
¡Delivery of the defined volume, including ejection of the re-
maining volume from the tip (Blowout)
Pipetting ¡Absorption of a defined volume
¡Delivery of the defined volume, including ejection of the re-
maining volume (Blowout)
¡Running the mixing cycles is possible
Repeat Dispensing ¡Absorbs a total volume as the sum of the defined sub-volumes
¡Dispensing the partial volumes in several preset steps. The re-
maining volume remains in the pipette tip.
¡Ejection of the residual volume (Blowout)
Sample Dilution ¡Absorbing a specified volume (sample)
¡Suction of an air bubble
¡Absorbing a second specified volume according to the desired
dilution ratio (diluent)
¡Dispensing the entire content of the tips
Reverse Pipetting ¡Absorbing a defined volume plus an additional volume
¡Dispensing the defined volume. The remaining volume remains
in the pipette tip.
¡Ejection of the residual volume (Blowout)
Serial Dilution ¡Absorption of a defined volume
¡Dispensing the defined volume in a specific volume of liquid
provided
¡Mixing the liquids
Change Tips ¡Motorized release of the tip holder
¡Removing the tip holder
¡Insertion of a new tip holder, equipped with tips
¡Motorized tightening and sealing of the tip holder

CyBio SELMA Technical description
19
3.9 The Pipetting Cycle
A pipetting cycle always consists of:
¡Aspiration (absorption of liquid)
¡Dispensing (dispensing of liquid)
¡Blowout (ejection of residual volume)
¡Return the plungers to the zero position
When the plunger has returned to the zero position, the next pipetting cycle can begin.
Aspiration and dispensing of liquid is accomplished by plunger motion. The plunger
drive moves a plate upwards and downwards, in which the plungers are loosely
mounted.
There is always an air cushion between the plunger and the liquid. The pipette tips are
airtight on the pipetting head. The movement of the plunger affects the air cushion
through this airtight seal. The raising and lowering of the plunger causes a negative or
positive pressure in the channel. The pressure differential causes the liquid to be aspi-
rated or dispensed.
The processes involved in pipetting and reverse pipetting are different. The following
graphics shows the different processes using the example of a single pipetting channel.
1
2
3
4
5
6
7
8
9
Fig.11 Elements of a pipetting head with 96 channels, one channel highlighted
1 Housing of the pipetting head 2 Mechanical guide for raising and lower-
ing the plungers
3 Attachment point of the channels 4 Sealing mat for sealing up the pipette
tips
5 Tip holder 6 Pipette tip
7 Channel 8 Plunger in the channel
9 Suspension of the plungers in the me-
chanical guide

Technical description CyBio SELMA
20
Pipetting
When pipetting, the device aspirates an exact volume of liquid without an additional
overstroke. The device delivers the volume with a blowout into the target cavity.
Zero-position
The plunger starts in the zero position above the source cavity. The pipette
tip is immersed in the liquid.
Aspiration
The plunger drive pulls the plunger upwards. The air cushion in the channel
creates a negative pressure during this movement. Liquid is aspirated into
the pipette tip by the negative pressure. The volume of the aspirated liquid
corresponds exactly to the nominal volume.
Dispense nominal volume and blowout
The dispensing takes place in the target cavity.
The plate moves the plunger down, compressing the air cushion in the chan-
nel. The resulting excess pressure pushes the liquid out. The device dis-
penses the nominal volume.
To empty the pipette tip completely, the mechanical guide moves the
plunger beyond the zero position (blowout). Residues of the liquid are
ejected by the blowout.