Endress+Hauser analytikjena Biometra TRobot II Manual

Setup Instructions
Biometra TRobot II
Automated thermal cycler

Biometra GmbH (Analytik Jena GmbH)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
E-Mail [e-mail]
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6 -10, -14 or -16
Fax + 49 551 50 68 6-66
E-Mail [e-mail]
http://www.analytik-jena.com
These setup instructions are valid from software version ME 2.00 – RE 2.00
For a proper and safe use of this product follow the instructions.
Keep the setup instruction for future reference.
34-4000-002-23
B (08/2021)
Analytik Jena GmbH
© Copyright 2021 Analytik Jena GmbH
Manufacturer
Service
General
information
Documentation number
Edition
Technical
documentation
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Biometra TRobot II Contents
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Contents
1Basic information ....................................................................................................... 5
1.1 Notes on this Setup Instruction ........................................................................................ 5
1.2 Intended use...................................................................................................................... 5
2Safety instructions...................................................................................................... 7
2.1 General notes .................................................................................................................... 7
2.2 Safety markings on Biometra TRobot II ........................................................................... 7
2.3 Technical condition ........................................................................................................... 8
2.4 Requirements for the operating personnel...................................................................... 8
2.5 Safety instructions: transport and installation ................................................................. 8
2.6 Safety instructions – during operation ............................................................................. 9
2.6.1 Summary of safety instructions ........................................................................................ 9
2.6.2 Safety instructions: protection against explosion and fire............................................... 9
2.6.3 Safety instructions: electrical equipment ......................................................................... 9
2.6.4 Handling of samples, auxiliary and operating materials ............................................... 10
2.7 Safety instructions – maintenance and repair ............................................................... 10
2.8 Behavior during emergencies ......................................................................................... 11
3Device installation and commissioning.................................................................... 12
3.1 Scope of delivery ............................................................................................................. 12
3.2 Unpacking and inspection............................................................................................... 12
3.3 Commissioning................................................................................................................ 12
3.3.1 Setting up the device....................................................................................................... 12
3.3.2 Connecting the cables ..................................................................................................... 14
3.4 Setting the operating voltage ......................................................................................... 15
3.5 Overview of the initial setup........................................................................................... 15
4Functional elements................................................................................................. 17
4.1 Biometra TRobot II PCR module front view ................................................................... 17
4.2 Biometra TRobot II PCR module rear view..................................................................... 18
4.3 Biometra TRobot II PCR controller front view................................................................ 19
4.4 Biometra TRobot II Controller rear view......................................................................... 19
5Operation.................................................................................................................. 20

Contents Biometra TRobot II
4

Biometra TRobot II Basic information
5
1Basic information
1.1 Notes on this Setup Instruction
As an excerpt from the detailed device instructions, these setup instructions contain the
first steps of installation and the safety instructions to be observed. For all further steps,
please apply the detailed operating instructions, which are supplied in electronic form
on a USB stick. Alternatively, you can also find the operating instructions on the Analytik
Jena homepage in the download area of the corresponding device.
Biometra TRobot II is intended for operation by qualified specialist personnel observing
the device operating manual and the operating manual for the Biometra TRobot II soft-
ware.
There are three different Biometra TRobot II models available. In the text below these
devices are collectively referred to as Biometra TRobot II. Differences are explained in
the corresponding chapters.
The operating manual contains information about the design and operation of Biometra
TRobot II and provides personnel familiar with PCR technology with the necessary
know-how for the safe handling of the device and its components. The operating man-
ual further includes notes on the maintenance and servicing of the device and potential
causes and remedies of any faults.
This instruction manual applies to all Biometra TRobot II devices from software version
ME 2.00 – RE 2.00.
Safety instructions are indicated by pictographs and signal words. The type and source
of the hazard are stated together with notes on preventing the hazard.
The operating manual uses the following symbols and signal words to indicate hazards
or instructions. Safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very
serious inju-
ries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTE
Provides information on potential material or environmental damage.
1.2 Intended use
Biometra TRobot II is an automated end-point thermal cycler. Biometra TRobot II was
specially developed for integration into automation systems, e.g., in liquid handling
Conventions
Symbols and
signal words used

Basic information Biometra TRobot II
6
systems. For space-saving integration on robotic decks, the thermal cycler is divided into
a block module and a separate controller. The block module is placed on or next to the
robotic platform, the controller, which is connected by cable, usually under the platform.
Biometra TRobot II can be operated from an automation platform software. For this
purpose, Biometra TRobot II comes with a software library (dll file) that can be inte-
grated into the automation software.
Alternatively, Biometra TRobot II can be controlled via the Biometra TSuite computer
software included in the scope of delivery.
To facilitate automatic work flows, the thermal cycler lid opens and closes automatically,
allowing a robot arm to place the sample plate on the block and remove it again after
the program has been completed. To ensure the plate can be removed smoothly, even
after many heating and cooling cycles which may have caused the PCR plate to shrink
slightly or become distorted, Biometrat TRobot II features a patent-pending plate lifting
mechanism: When the lid is opened, the plate is slightly lifted up by four small pins that
move out of the block, thus detaching it from the block. This allows a robot arm to easily
remove the plate from the block.
A status LED at the front of the block module indicates the respective status of the ther-
mal cycler by means of color and luminous frequency.
Like a typical thermal cycler, the Biometra TRobot II automated thermal cycler is also
primarily designed to amplify nucleic acids by repeated heating and cooling cycles using
DNA polymerases in a PCR reaction. Other incubation of liquid samples in PCR tubes or
plates is also possible.
Deviations from the intended use described in this short instruction and in the operating
manual result in limitations of warranty and liability during a damage event. Damage to
wearing parts is not included in the warranty.
If the safety instructions given in this setup instruction and in the operating manual are
not observed when handling the device, this is considered a deviation from the intended
use.
The thermal cycler is developed for Research Use Only (RUO).

Biometra TRobot II Safety instructions
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2Safety instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of Biometra TRobot II,
please read this chapter carefully before commissioning.
Besides the safety instructions in the operating manual and the local safety regulations
that apply to the operation of the device, the general applicable regulations regarding
accident prevention, occupational health and safety, and environmental protection have
to be observed and complied with.
References to potential hazards do not replace the work protection regulations which
must be observed.
2.2 Safety markings on Biometra TRobot II
Damaged or missing safety symbols can cause incorrect actions leading to personal in-
jury or material damage. Do not remove safety symbols! Replace damaged safety sym-
bols immediately!
The following safety symbols are attached to the inside of the heated lid and the rear of
the device:
Symbol Meaning Notes
Caution!
Hazard point
This symbol is located on the inside of the heated lid and on
the rear of the device.
Proceed with due care when handling samples and working
with Biometra TRobot II.
Caution!
Risk of burns
This symbol is located on the inside of the heated lid and on
the rear of the device.
There is a risk of burning at the heated lid, the thermal block,
the samples, and the rear of the device.
Ensure ventilation slots are
unobstructed
This sign is attached to the underside of the device.
Ensure that the ventilation slots on the underside and at the
rear of the device are unobstructed. Make sure that there are
no objects underneath the device (e.g., paper) that may be
sucked against the ventilation slot and thus interfere with
ventilation.
Biometra TRobot II must not be placed with the device feet
in recesses in higher base plates as it will cause ventilation
from the underside of the device to be restricted. Recesses
for the device feet as positioning aids on a platform may be
max. 1 mm deep.

Safety instructions Biometra TRobot II
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2.3 Technical condition
Biometra TRobot II has been built and certified according to safety standard EN 61010-
1. Do not modify the device in any way. Any modification made to the device will lead to
a loss of warranty and the EN 61010-1 certificate and poses a potential risk.
The following has to be observed:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating per-
sonnel to the operator without delay.
2.4 Requirements for the operating personnel
Biometra TRobot II must only be operated by qualified specialist personnel instructed in
the use of the device. The instruction also includes imparting the contents of the operat-
ing manual.
In addition to the safety at work instructions in the operating manual, the generally ap-
plicable safety and accident prevention regulations of the respective country of opera-
tion must be observed and adhered to. The operator must ascertain the latest version of
these regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
Drain the sample block before transporting Biometra TRobot II. Make sure there are
no sample tubes in the block.
Only send Biometra TRobot II in its original packaging.
On receipt of Biometra TRobot II, please verify that the delivery is complete and
check for possible transport damage. In the event of a claim, please contact Analytik
Jena.
The Biometra TRobot II is designed for indoor use only and not for installation and
operation in a wet environment.

Biometra TRobot II Safety instructions
9
2.6 Safety instructions – during operation
2.6.1 Summary of safety instructions
Before each commissioning, the operator of Biometra TRobot II must make sure that the
condition of the device including the safety equipment is sound.
The following has to be observed:
Free access to the main power switch on the rear panel of device must always be en-
sured during operation.
The ventilation fittings at the rear of the device must be unobstructed and opera-
tional. Covered ventilation grilles or slots etc. may cause the device to break down or
may cause damage to it.
The use of oil between the samples and the sample block is not necessary to achieve
an improved heat exchange. However, if you still want to use oil, you should use
mineral oil. Do not use silicone oil.
Be careful to avoid crushing or pinching injuries when closing the device.
Biometra TRobot II may cause burns. The following has to be observed:
The thermal block, the samples, and the heated lid reach high temperatures. There is
a risk of burns during contact.
Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
Do not touch hot sample tubes or plates and do not open them or boiling liquid may
escape!
Only use plates and tubes that are suitable for high temperatures (up to 100 °C), fit
well into the thermal block (no wobbling), and whose lids seal tightly!
Modifications and extensions to the device may only be carried out after consultation
with Analytik Jena. Unauthorized changes can limit the operating safety and lead to
restrictions in warranty and access to customer service.
2.6.2 Safety instructions: protection against explosion and fire
Biometra TRobot II must not be operated in an explosive environment. Biometra TRobot
II must not be operated with flammable, explosive, or volatile substances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of Biometra TRobot II may only be performed by a
qualified electrical technician in accordance with the applicable electrical engineering
rules. Life-threatening electrical voltages may occur in the interior of Biometra TRobot
II!
The following has to be observed:
Any work on the interior of the device may only be carried out by Analytik Jena ser-
vice department and specially authorized technicians.

Safety instructions Biometra TRobot II
10
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty, or damaged cables, must be repaired with-
out delay.
Before opening the device it must be switched off at the main power switch and the
power plug must be disconnected from the power outlet!
Biometra TRobot II must be switched off immediately using the main power switch
(on the rear panel of the controller) and the power plug disconnected from the
power supply if any faults occur in the electric components.
Do not operate the device near sources of strong electromagnetic radiation (e.g., a
non-shielded, deliberately operated high frequency source), as these may affect
proper operation of the device.
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as
for their safe handling. This is particularly important for radioactive, pathogenic, infec-
tious, poisonous, corrosive, or otherwise hazardous substances. For details contact the
safety officer responsible for your location. When handling hazardous substances local
safety codes and guidelines must be observed. The following general notes do not re-
place the specific local regulations or the regulations in the safety data sheets of the
manufacturers for the auxiliary and operating materials.
The following has to be observed:
Protective goggles and rubber gloves have to be worn when handing reagents.
If only a few samples are to be treated, two (empty) tubes of the same height must
additionally be placed in the corner positions of the block. If the number of samples
in the block is too low, there is a risk of the tubes being damaged and sample liquid
leaking out.
For your own safety, please consider the potential risk of infection of the examined
biological material.
2.7 Safety instructions – maintenance and repair
Biometra TRobot II is usually repaired by the Biometra GmbH/Analytik Jena AG service
department or by authorized and trained specialist personnel. Unauthorized repairs can
damage the device. Therefore, the operator may generally only carry out the tasks listed
in the operating manual.
The following has to be observed:
The exterior of the device may only be cleaned with a damp, not dripping, cloth after
the device has been switched off.
Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).
Use only original spare parts or consumables. Only these parts are tested and guar-
antee safe operation.
If the instrument is to be sent to the service department, please contact the Bi-
ometra/Analytik Jena service department beforehand. You will then receive an au-
thorization number. In addition, the device must be decontaminated before sending

Biometra TRobot II Safety instructions
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it in and the corresponding form must be attached to the outside of the packaging,
see note in the detailed operating instructions.
2.8 Behavior during emergencies
During hazardous situations, Biometra TRobot II must be switched off immediately by
operating the main power switch at the rear left of the controller. Disconnect the power
plug from the power supply!

Device installation and commissioning Biometra TRobot II
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3Device installation and commissioning
3.1 Scope of delivery
Thermal cycler:
PCR module + controller

Power cable

1 supply cable and 1 signal cable, fixed
to the controller

1 RS232 cable

Screwdriver for opening the lid
manually in the event of a power failure
Biometra TRobot II Setup Instructions
(print)

Biometra TRobot II operating manual,
Biometra TSuite software operating
manual, BiometraLibrary manual (elec-
tronic, on Biometra TRobot II USB stick)

Biometra TSuite computer software (on
Biometra TRobot II USB stick)

Biometra TSuite single license as USB
copy protection key
3.2 Unpacking and inspection
Unpack the device and check that it is complete and undamaged. Immediately report any
damage to Analytik Jena/Biometra.
WARNING
Do not commission the device if there are any signs of
damage.
Keep the original packaging for return transport in the event that servicing is required.
The shipping box for the Biometra TRobot II thermal cycler contains specially developed
molded parts for the safe transport of high quality electronic devices.
3.3 Commissioning
3.3.1 Setting up the device
Place the Biometra TRobot II thermal cycler on a solid, non-flammable surface in a
secure and dry environment. Consider the specific ambient conditions: 15 °C to
30 °C, max. 70 % air humidity, max. 2000 m above sea.
Allow the Biometra TRobot II thermal cycler to equilibrate to room temperature be-
fore switching it on.
Ensure adequate ventilation around the device, otherwise the device's performance
may be impaired. A minimum distance of 20 cm should be maintained behind the
thermal cycler between the rear of the device and a wall or other objects.
If several thermal cyclers stand next to each other and are operated simultaneously,
we recommend a lateral distance of at least 10 cm between the devices.

Biometra TRobot II Device installation and commissioning
13
Make sure that the power switch on the controller and the power cable are easily ac-
cessible. This is important if the device needs to be disconnected from the power
supply.
Make sure that the ventilation slots at the sides and the bottom of the device are un-
obstructed.
Fig.
1 PCR module rear view
Fig.
2 Controller rear view
Make sure that there are no objects underneath the device (e.g., paper) that may be
sucked against the ventilation slot and thus interfere with ventilation.
Insufficient ventilation may cause the de-
vice to overheat.
Biometra TRobot II must not be placed with the device feet in recesses in higher base
plates as it will cause ventilation from the underside of the device to be restricted.
Recesses for the device feet as positioning aids on a platform may be max. 1 mm
deep.
Before switching on the device, make sure that the power supply corresponds to the
device setting (see section "Setting the operating voltage" p. 15).

Device installation and commissioning Biometra TRobot II
14
3.3.2 Connecting the cables
Connect the Biometra TRobot II thermal cycler controller to a grounded power outlet
using the power cable supplied. The cable must not be replaced by an inadequately
dimensioned power cable.
Connect the supply cable (DSub 25) and the signal cable (DSub 24W7), which are
fixed to the controller, to the connectors on the rear of the PCR module. Both cables
are 3 m long. It is not possible to mix up the connectors inadvertently, as the two
connectors are different.
Fig. 3 Cables and connectors for connecting the PCR module to the controller.
Left: DSub 24W7 signal cable, right: DSub 12 supply cable (cable colors may vary)
Connect the controller directly to the computer if you want to use the RS232 connec-
tion.
If your computer does not have an RS232 interface, you can use a USB-to-RS232
adapter together with the enclosed RS232 cable. As this connection is a point-to-
point connection, it is very insensitive to interference.
Alternatively, you can also connect the controller to a computer via your home net-
work. The Biometra TRobot II is configured on delivery to a network connection with
a DHCP server.
An Ethernet cable is required for this (minimum requirements: performance class Cat
5e, cable structure STP, max. length 30 m).
You connect the Biometra TRobot II and the computer via the ethernet cable
and a router with a DHCP server (e.g. such a device https://www.tp-
link.com/us/home-networking/wifi-router/tl-wr841n/), alternatively you
can connect the computer and Biometra TRobot II to the company network
if this is allowed according to your company IT regulations.
For an alternative direct connection of the device (exclusively with a com-
puter via a network cable), the device must be changed via Biometra TSuite
either to a network connection with DHCP or via a serial connection to a
network connection with a fixed IP (e.g. IP 192.168.0.30, subnet mask
255.255.255.0). See also Manual Biometra TSuite Software for support.

Biometra TRobot II Device installation and commissioning
15
The network card must also be changed to a fixed IP(V4) (e.g. IP
192.168.0.10, subnetmask 255.255.255.0) via the network adapter set-
tings under Windows.
The PC network card should comply with the auto-crossover standard IEEE
803.2ab_1999, otherwise a crossover network line is required. Newer net-
work cards usually meet this standard.
An Ethernet cable and a USB-to-R232 adapter are available as optional accessories.
Check the operating voltage before switching on the device!
3.4 Setting the operating voltage
WARNING
Danger of electric shock! Before commissioning the device, make sure that the voltage
selector setting on the
underside of the device corresponds to the power supply in your
laboratory.
The Biometra TRobot II thermal cycler can be operated at 100, 115 or 230 volts. The
current setting is indicated on the voltage selector switch on the underside of the con-
troller.
To change the operating voltage, switch off the device and unplug the power plug.
Use a coin or other round object to rotate the voltage selector switch slot and adjust
the new operating voltage.
3.5 Overview of the initial setup
In the following, the most important steps are summarized once again:
1. Connect the controller to a power outlet with the power cord.
2. Connect the two cables of the Biometra TRobot II Controller to the PCR module.
3. Connect the controller to the computer via Ethernet, RS232 or USB-to-RS232
adapter.
4. Turn on the controller.

Device installation and commissioning Biometra TRobot II
16
5. If the Biometra TRobot II is controlled by the Biometra TSuite software (see Bi-
ometra TSuite Software User Manual for details), then:
a. Install the Biometra TSuite software with the “Biometra TSuite.exe”
(from USB flash drive).
b. Start the Biometra TSuite software
c. Choose the section “SETTINGS“ an and select the used inter-
face, e.g. “SERIAL INTERFACE”.
6. Go back to “HOME SCREEN”.
7. the software automatically scans for connected thermal cyclers and displays the
devices found after a few seconds.

Biometra TRobot II Functional elements
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4Functional elements
4.1 Biometra TRobot II PCR module front view
Fig. 4 Biometra TRobot II PCR module front view
1 Seal for the block
2
Heated lid
3
Plate lifter
4
Sample block (96-well/silver)
5 Status LED
6
Safety frame

Functional elements Biometra TRobot II
18
4.2 Biometra TRobot II PCR module rear view
At the rear of the Biometra TRobot II there are the connectors for the DSub 24W7 signal
cable and for the DSub 12 supply cable.
Fig. 5 Biometra TRobot II PCR module rear view
1 Signal cable
2
Supply cable
3 Ventilation slots
4 Slot for lid opening mechanism

Biometra TRobot II Functional elements
19
4.3 Biometra TRobot II PCR controller front view
The controller does not have any operating functions other than the power switch lo-
cated at the rear of the device on the left.
Fig. 6 Biometra TRobot II controller rear view
4.4 Biometra TRobot II Controller rear view
Fig. 7 Biometra TRobot II controller rear view
1 Serial RS232 port
2
Signal cable port
3
Ethernet port
4
Supply port for PCR module
5 Ventilation slots
6
Power switch
7
Power supply connection

Operation Biometra TRobot II
20
5Operation
For further information please check out the operating manuals of the device and of the
software. These manuals are included in electronic form on USB flash drive. Alterna-
tively, you can also find them on the Analytik Jena homepage in the download area of
the corresponding device.