GYROZEN 416 User manual

Low-Speed Centrifuge
416
Operation Manual
GYROZEN Co., Ltd.
www.gyrozen.com
UM-416(E)(Rev. )
1F & 5F, 16 Arayuk-ro, Gimpo-si, Gyeonggi-do, KOREA
Tel) [phone], FAX) [phone]
2

2
Low-Speed Centrifuge 416
CONTENTS Page #
1. Meanings of Symbols & Safety Precautions ..................................................................................................3
1-1 Meanings of Symbols ................................................................................................................................3
1-2 Safety Precautions ....................................................................................................................................3
2. Product Description & Technical Specifications ...........................................................................................5
2-1 Product Description ...................................................................................................................................5
2-2 Technical Specifications ............................................................................................................................5
3. Unpacking ......................................................................................................................................................6
4. Installation ......................................................................................................................................................7
4-1 Power ON / OFF and Door Release ..........................................................................................................7
4-2 Rotor Coupling and Disassembling.............................................................................................................7
4-3 Positioning of Sample Tubes ......................................................................................................................8
5. Operation .......................................................................................................................................................9
5-1 Key Functions of Control Panel .................................................................................................................9
5-2 Setting the RPM / RCF Value ....................................................................................................................9
5-3 Setting the Time Value..............................................................................................................................10
5-4 START / STOP ........................................................................................................................................10
5-5 SOFT START / STOP...............................................................................................................................10
5-6 Program SAVE / RECALL.........................................................................................................................10
5-7 Emergency Door Release.........................................................................................................................11
5-8 Replacement of Fuse................................................................................................................................11
6. Maintenance ................................................................................................................................................12
6-1 Outer part of instrument ..........................................................................................................................12
6-2 Chamber .................................................................................................................................................12
6-3 Shaft ........................................................................................................................................................12
6-4 Rotor .......................................................................................................................................................12
6-5 Transportation of the instrument ...............................................................................................................12
7. Troubleshooting .........................................................................................................................................13
7-1 Checklist ..................................................................................................................................................13
7-2 Error Codes...............................................................................................................................................13
8. Rotors and Accessories ...............................................................................................................................15
9. Product Range .............................................................................................................................................19
10. CE ..............................................................................................................................................................20

3
Low-Speed Centrifuge 416
1. Meanings of Symbols & Safety Precautions
1-1. Meanings of Symbols
1-1-1. Symbols on the device
Symbol
Meaning
Symbol
Meaning
Attention and warning.
Attention and warning for
electric shock
Attention and warning for
rotor coupling.
Attention and warning for
door opening and closing
Attention and warning for
correct way of sample
balancing in the rotor.
Attention and warning for
correct way of buckets
position.
Indicate a hole for
manual door opening in
case of emergency
1-1-2. Symbols in this document
Symbol
Meaning
Symbol
Meaning
This symbol refers to safety
relevant warnings and indicates
possible dangerous outcomes.
☞
Note.
This symbol refers to the important
reminder.
1-2. Safety Precautions
Before using the instrument, please read this operation manual to ensure correct usage. Incorrect handling of the
instrument may possibly result in personal injury or physical damage on the instrument or its accessories.
1. ALWAYS locate the instrument on a flat, rigid and stable table capable of withstanding the weight of the
instrument and its spinning operation.
2. ALWAYS make a safety zone of 30 cm around the instrument to indicate that neither hazardous
materials nor persons should be permitted within the area during operation.
ALWAYS position the instrument with enough space on each side of instrument to ensure
proper air circulation.
3. ALWAYS install the instrument within a temperature and humidity controlled environment (Permissible
ambient temperature: 5 ~ 35 °C, Relative humidity: ≤85%).

4
Low-Speed Centrifuge 416
4. Before connecting the main power, check the rated voltage.
5. Should not use incompatible rotors and accessories.
Only use the rotors manufactured by GYROZEN Co., Ltd., appropriate centrifugal tubes and
adaptors to hold sample containers tight in the rotors.
6. Before operating the instrument, check if the rotor and the lid are securely fastened.
Should operate the instrument with a compatible rotor properly installed and secured to the
motor shaft.
7. Mount the rotor on the motor shaft properly, check it by spinning manually.
8. Do not stop the rotor by hands while spinning.
9. Manual door release is available only when spinning of the rotor is completely stopped.
10. Should not exceed the rated rotational speed or specific gravity. If the loaded samples have densities
of over 1.2 g/ml, the maximum rotational speed should be kept lower to prevent from rotor failure.
11. The sample content should not exceed 80% of total capacity of a tube. Otherwise, it may cause
spillage of sample or even the tube breakage.
12. ALWAYS load the sample tubes symmetrically with evenly weighted samples not to cause rotor
imbalance. If necessary, use the water blank to counterbalance the unpaired sample.
13. The rotation speed should not exceed the highest value of the individual guaranteed g-forces for
centrifuge, rotor, bucket or adaptor. Especially, the guaranteed g-strength of the sample container
should not be neglected.
14. The rotors should be cleaned and kept dry after use, for longer life span and safety.
15. ALWAYS disconnect the power supply prior to maintenance care and service to avoid electrical shock.
16. ALWAYS use proven disinfection procedures after centrifuging biohazardous materials.
17. Should not centrifuge flammable, toxic, radioactive, explosive or corrosive materials.
18. When it is necessary to use toxic or radioactive materials or pathogenic microorganisms which belong
to the Risk Group II of WHO: “Laboratory Bio-safety Manual,” should follow national regulations.
Do not place dangerous materials within 30 cm distance around the instrument, and that is
also recommended by IEC 61010-2-020.
Use the Emergency Door Release function only when the door button on the control panel
is dumb under the condition of complete stop of rotor running.
Never attempt to open or move the instrument until it is completely stopped.
Power input of 10% greater or less than the recommended voltage or fluctuates frequently
may cause malfunction of the instrument and often serious damage.
Install the instrument at the place free from any kinds of corrosive gases.

5
Low-Speed Centrifuge 416
2. Product Description & Technical Specifications
2-1. Product Description
2-2. Technical Specifications
Max. RPM / RCF
4,000 rpm / 2,700 xg
Max. capacity
Fixed angle
16 x 15 ml
Swing-out
4 x 100 ml
Time control
Timed < 100 min or continuous
RPM / RCF conversion
Yes
Noise level (dB)
≤52
ACC / DEC (sec)
≤20 / ≤ 25
SOFT START / STOP
Yes

6
Low-Speed Centrifuge 416
Program memory
10
Imbalance cutout
Yes
Safety lid lock
Yes
Door drop protection
Yes
Automatic door release at completion
Yes
Power supply (V/Hz)
220 / 50~60 (110V optional)
Power requirement (VA)
340
Dimension (W x D x H, mm)
375 x 480 x 260
Weight without rotor (kg)
19.5
CE mark
Yes
Cat. No.
GZ-0416
☞The instrument has the following functions for safety.
1. SOFT spin key for gentle acceleration and deceleration.
2. Automatic detection and alarms for imbalance, excess speed and heating.
3. Unpacking
Motor Fixing Cushion in 416
☞The cushion should be removed before the installation of the instrument.
Motor Protecting Device: a motor fixing cushion (Polyethylene (PE) foam) is placed in the chamber
of 416 centrifuge to prevent possible damages to the imbalance sensor caused by motor shaking
during shipping or relocation.

7
Low-Speed Centrifuge 416
4. Installation
4-1. Power ON / OFF and Door Release
Action
4-1-1. Power ON / OFF
1. Connect the AC Power cord to the power socket on the right back of the
instrument.
2. Turn on the instrument by pressing the switch on the right side of the
instrument.
3. Press the [DOOR] button to open the door.
4-1-2. Door Release
1. To open the door, press the [DOOR] button.
The door is automatically opened with end alarm
upon completion of the rotation.
Close the door until you hear the door clank.
When the door is opened, the door LED turns on.
☞
The door is not opened while the instrument is running.
If the door is open, the instrument does not run by pressing the [START] button.
Power Failure: If there is any power failure during operation, door is not opened with [DOOR] button.
Door can be opened only when the rotation completely stops and the power is on again. If you want
to open the door when power is gone off, please refer to ‘5-7. Emergency Door Release’.
4-2. Rotor Coupling and Disassembling
Action
Before coupling a rotor, clean the motor shaft and chamber with soft dry
towel.
4-2-1. Swing-Out Rotor
1. Mount a compatible rotor into the motor shaft.
2. Grasp the rotor with one hand, and place Rotor Locking Tool
at the center hole of the rotor.
To assemble the rotor, rotate the Rotor Locking Tool
clockwise until tightly assembled.
To disassemble the rotor, rotate the Rotor Locking Tool
counterclockwise.
3. Hang the appropriate buckets into the rotor.
Load an identical bucket at each wing (Do not leave any
vacant wing without bucket. All wings should hold identical
bucket.).
Remove dirt or dust around hooks of rotor and hanging part
of bucket.

8
Low-Speed Centrifuge 416
4. Spin the rotor manually to check if bucket swinging is sufficiently smooth. If the rotation is resisted,
apply the lubricant (grease) to the link area.
4-2-1. Fixed Angle Rotor
1. Mount a proper rotor into the motor shaft. Put the Washer ( ) at
the center hole of the rotor and assemble it with the Rotor Locking Nut
( ).
To assemble the rotor, rotate the Rotor Locking Nut
clockwise until tightly assembled.
To disassemble the rotor, rotate the Rotor Locking Nut
counterclockwise.
2. Load the 15-ml sleeves in every hole.
When you couple the rotor at first installation, you turn
off the instrument.
After coupling the rotor, turn on the instrument.
4-3. Positioning of Sample Tubes
Action
1. Before loading sample tubes, check if there are any water drops or dirt in the rotor hole or tube adaptors.
If you find any, remove it with soft dry cloth.
2. The sample tubes should be loaded symmetrically with the density and the weight considered to avoid
imbalance.
Only use appropriate centrifugal tubes and do not exceed the speed beyond the tube’s max
g-strength.
Correct Way of Sample Balancing and Tube Usage
In case the number of samples cannot make balance in weight, please use control
tubes. Otherwise, it can cause noise or vibration, which may damage the instrument.
☞For safety, the ‘Imbalance Cutoff’ function is turned on, when there is weight imbalance of the loaded tubes
(Error 8, Imbalance error). Please refer to 7. Troubleshooting.

9
Low-Speed Centrifuge 416
5. Operation
5-1. Key Functions of Control Panel
Setting Speed
□ RPM / RCF To set the speed and for automatic conversion between RPM and RCF
Setting Time
□ TIME To set time up to 99 min (0:00:00: continuous)
Setting Functions
□ PROG To save the set values and recall the saved programs
□ START / STOP To start or stop operation
□ DOOR To open the instrument lid
□ SOFT START / STOP To operate with slower and smoother acceleration / deceleration
5-2. Setting the RPM/RCF Value
▶Maximum RPM / RCF: 4,000 RPM / 2,700 xg
☞In order to increase or decrease values rapidly, keep either of the [▲▼] buttons pressed over 5 seconds.
Action
5-2-1. Setting the RPM Value
▶Speed setting unit: 10 rpm
1. Press a [RPM/RCF] button once.
RPM MODE is on by pressing the [RPM/RCF] button
once.
2. Press the [▲▼] buttons to change the set value.
3. Press the [RPM/RCF] button again to set the value.
5-2-2. Setting the RCF Value
▶Speed setting unit: 1 xg
Press a [RPM/RCF] button twice.
RCF MODE is on by pressing the [RPM/RCF] button
twice.
1. Press the [▲▼] buttons to change input value.

10
Low-Speed Centrifuge 416
2. Press the [RPM/RCF] button again for saving.
5-3. Setting the Time Value
▶Speed setting unit: 1 min / 1 sec
Action
5-3-1. Setting the MIN Value
1. Press the [TIME] button once.
The MIN mode is on by pressing the [TIME] button once.
2. Press the [▲▼] buttons to change the set value.
3. Press the [TIME] button again to set the value.
5-3-2. Setting the SEC Value
1. Press the [TIME] button twice.
The SEC mode is on by pressing the [TIME] button twice.
2. Press the [▲▼] buttons to change the set value.
3. Press the [TIME] button again to set the value.
5-4. START / STOP
Action
1. After setting the RPM/RCF and the TIME, press [START/STOP]
button.
While running, the ‘START LED’ is turned on.
To stop the operation, press the [START/STOP] button while running.
5-5. SOFT START / STOP
The [SOFT START/STOP] button is used for gentle acceleration and deceleration for sensitive samples.
Action
1. After setting TIME and RPM, press the [SOFT START/STOP]
button once.
The LED of [SOFT START/STOP] button is on
while running.
The door is automatically opened when the operation is completed.
When operating in the [SOFT] mode, the operation cannot be stopped by pressing the
[START/STOP] button.
5-6. Program Save / Recall
Action
5-6-1. Program Save
1. Set parameters (Refer to 5-2 ~ 5-3).