Optika Italy IM Series User manual

Ver. 1.1 2022
Model
M-797
M-798
IM Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 M-797 4
3.2 M-798 5
4. Unpacking 6
5. Intended use 6
6. Symbols and conventions 6
7. Instrument description 7
7.1 M-797 7
7.2 M-798 8
8. Assembling 9
8.1 Installingthelterholder 9
8.2 Installingtheuorescencelters 9
8.2.1 M-797 9
8.2.1 M-798 10
8.3 Installingtheltercovers 10
8.4 Installingthelterselectionlever(M-798) 10
8.5 Installing the epi-illuminator 10
8.6 Installing the lamp housing 11
8.7 Installing the bulb 11
9. Useofthemicroscopeinfluorescence(reflectedlight) 13
9.1 Centering the mercury bulb 13
9.2 Centeringtheelddiaphragm 15
9.3 E󰀨ectsoftheelddiaphragm 15
9.4 Availableuorescenceltercubes 15
10. Simultaneous observation in Phase Contrast + Fluorescence 16
Equipment disposal 17

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It is
built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains im-
portant information on safety and maintenance, and that it must therefore be made accessible to the instrument users. We
decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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① Fluorescencepowersupply
② Lamphousing
③ Epi-illuminator
④ 2positionlterholder
⑤ Fluorescencelters(B-G)
⑥ Slidercovers
⑦ HBObulb
⑧ UVshield
⑨ Fluorescenceconnectioncable
⑩ Powercord
3. Package content
3.1 M-797
⑩③④
⑤
⑥
⑦
⑧
⑨
①②

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3.2 M-798
① Fluorescencepowersupply
② Lamphousing
③ Epi-illuminator
④ 4positionlterholderwithltersincluded(B-G)
⑤ Filtersselectorlever
⑥ Slidercovers
⑦ HBObulb
⑧ UVshield
⑨ Fluorescenceconnectioncable
⑩ Powercord
⑩③
④
⑤
⑥
⑦
⑧
⑨
①②

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get damaged.
Using both hands (one around the arm and one around the base), lift the microscope from the container and put it on a
stable desk.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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7. Instrument description
7.1 M-797
FLUORESCENCE
POWER SUPPLY
UV SHIELD
LAMP HOUSING
FLUORESCENCE
CUBE LEVER (ONE ON
BOTH SIDES)

Page 8
LAMP HOUSING
UV SHIELD
7.2 M-798
FLUORESCENCE
CUBE LEVER
FLUORESCENCE
POWER SUPPLY

Page 9
8. Assembling
FFig. 1ig. 1
①
②
FFig. 2ig. 2
FFig. 3ig. 3
③
FFig. 4ig. 4
8.1 Installingthelterholder
1. Unscrew the screw ①on both side of the microscope to re-
move the plastic covers ②. (Fig. 1)
2. Unscrew the three screws ③holding the plate on the micro-
scope body. (Fig. 2)
3. Insert the lter holder (either 2 positions or 4 positions) in the
microscope, with the “L” facing the back side of the micro-
scope. (Fig. 3)
4. Fix the lter holder by screwing the three screws ③, removed
before from the plate.
8.2 Installingtheuorescencelters
8.2.1 M-797
Insert the lter cubes by sliding them from the sides of the micro-
scope: B from the left and G from the right. (Fig. 4)
• Payattentiontoinstalltheltercubeswiththeemission
lterfacingdown.
③
③③

Page 10
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
8.2.1 M-798
Insert the lter holder by sliding from the side of the microscope.
The insertion side is not relevant. (Fig. 5)
• Payattentiontoinstallthelterholderwiththeemission
lterfacingdown.
8.3 Installingtheltercovers
1. Once the uorescence lters are in place, cover the lter
holder with the provided covers.
• For M-797: the two cover plates have the same shape and
can be mounted either on the left or on the right.
• For M-798: install the cover with the printed encryption on the
left side of the microscope. (Fig. 6)
8.4 Installingthelterselectionlever(M-798)
Screw the lter selection lever inserting the lever into the hole of
the left cover. (Fig. 7)
8.5 Installing the epi-illuminator
1. Remove the black plastic cover from the microscope rear.
2. Insert the uorescence illuminator from the back. In order
to ease the insertion, just tilt the assembly at about 45° and
move it forward.
3. Fix it using the 3 provided Allen screws. (Fig. 8)
FFig. 8ig. 8

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8.6 Installing the lamp housing
Insert the lamp house and x it with the Allen screw ①. (Fig. 9)
8.7 Installing the bulb
1. Open the lamp housing using the door lock screw ② and
remove the lamp holder. (Fig. 10)
2. Remove the plastic block ③ from the lamp holder (or the
exhausted lamp in case of replacement) by loosening the two
locking screws ④. (Fig. 11)
FFig. 9ig. 9
①
FFig. 11ig. 11
③
④
④
FFig. 10ig. 10
②
FFig. 12ig. 12
⑤
3. Insert the mercury bulb ⑤ (respect the polarity of the bulb),
tighten the locking screws and ret the lamp holder inside the
lamp housing. (Fig. 12)
• Donottouchthebulbwithbarehands.

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4. Connect the cable from the external power supply to the
lamp housing. (Fig. 13)
5. Connect the power cable to the external power supply. (Fig.
14)
• The input voltage for uorescence power
supply is 110-240Vac.
• Please use the standard power cable pro-
vided. Select suitable one when missing or
damaged.
• Connectthepowersupplycorrectly,besure
to have a good earth connection.
• Before connecting the power cord, secure
thelamphousingcabletothepowersupply.
• Ifthepowercordisconnectedbefore,there
may be a risk of electrical shock.
• Disconnect all electrical cables before
installing or replacing the bulb.
• The bulb has an anode and a cathode of
di󰀨erent sizes. Respect the polarity during
assembly.
FFig. 13ig. 13
FFig. 14ig. 14
6. In order to prevent possible damages from UV radiation,
mount the orange protection screen as shown. (Fig. 15)
FFig. 15ig. 15

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9. Useofthemicroscopeinfluorescence(reflectedlight)
9.1 Centering the mercury bulb
• Wait around 5 minutes before proceeding with this
operationtoallowthebulbtoproperlywarmup.
1. Turn on the HBO mercury bulb by operating the power supply
switch ①. (Fig. 16)
2. Turn the nosepiece into an empty position (without objectives)
and remove the protective cap, or remove an objective from
the nosepiece.
FFig. 16ig. 16
①
FFig. 17ig. 17
FFig. 18ig. 18
FFig. 19ig. 19
②
③
③
3. Place a piece of white paper on the stage and insert the lter
cube “B” into the optical path. (Fig. 17)
4. Acting on the focus screw of the collector lens ② and on the
centering screws ③ try to obtain the light spot of the bulb’s
arc. (Fig. 18-19)
5. Using the focus screw of the collector lens ②, put the image
of the arc projected onto the paper. The light spot must be
brighter and sharper as possible. (Fig. 19)

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6. Using the centering screws ③ on the side of the lamp
housing, center the image of the arc. (Fig. 20-21)
FFig. 20ig. 20
FFig. 21ig. 21
FFig. 22ig. 22
FFig. 23ig. 23
①
7. Using the focusing screw of the collector lens ② enlarge the
image until a homogeneous illumination is achieved. (Fig.
22). At this point, insert an objective into the optical path and,
looking into the eyepieces, optimize the illumination always
using the screws ② and ③.
8. After replacing the exhausted bulb, reset the time counter on
the power supply by pressing the “Reset” button ①. (Fig. 23)

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9.2 Centeringtheelddiaphragm
1. Place the specimen on the stage, insert 10x objective into the
light path and focus.
2. Rotate the eld diaphragm lever ①, to fully close the
diaphragm. (Fig. 24)
3. Rotate the two centering screws ②to bring the bright spot in
the center of the eld of view.
4. Gradually open the diaphragm. The eld diaphragm is
centered when the diaphragm image is symmetrical to the
eld of view.
5. In normal use, open the diaphragm until it circumscribes the
eld of view. FFig. 24ig. 24
①
②
FFig. 25ig. 25
9.3 E󰀨ectsoftheelddiaphragm
Field diaphragm adjusts the illuminated area to obtain a high
contrast image.
Set the diaphragm according to the objective in use until it
circumscribes the eld of view, in order to eliminate unnecessary
light to eyepieces. (Fig. 25)
9.4 Availableuorescenceltercubes
• M-797
FILTER
NAME EXCITATION FILTER DICHROIC MIRROR BARRIER FILTER APPLICATIONS
B 460-490 nm 495 nm 520LP nm
• FITC: uorescent antibodies
• Acridine orange: DNA, RNA
• Auramine
G540-580 nm 585 nm 590LP nm
• Rhodamine, TRITC: uorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
• M-798
FILTER
NAME EXCITATION FILTER DICHROIC MIRROR BARRIER FILTER APPLICATIONS
B 460-490 nm 500 nm 520LP nm
• FITC: uorescent antibodies
• Acridine orange: DNA, RNA
• Auramine
G527-553 nm 565 nm 575LP nm
• Rhodamine, TRITC: uorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
UV 325-375 nm 415 nm 435LP nm • Nuclear counterstaining
V 390-420 nm 440 nm 455LP nm • Acridine orange: DNA, RNA

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10. Simultaneous observation in Phase Contrast + Fluorescence
• FluorescencemodelsallowobservationintransmittedlightPhasecontrastincombinationwithreectedlight
Fluorescence.SampleswithrapiddecaymustrstbeobservedinFluorescenceandtheninPhaseContrast.
Thecombinedobservationallowsyoutoeasilyidentifysomeareasofthesamplethatemituorescence.
1. Turn on the power supply for the HBO uorescent bulb and wait 5 minutes before the arc stabilizes.
2. Move the lter selector to an empty position or, if the lter holder is full, to the position containing the UV lter.
3. Insert the desired PH objective and move the phase contrast slider to the position containing the corresponding phase
ring.
4. Focus the sample.
5. Adjust the light intensity of the transmitted light.
6. Move the uorescence lter selector to the desired position.
7. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate the intensity
of the uorescence with the one of the phase contrast.

Page 17
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sep-
arately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to pre-
vent possible adverse e󰀨ects on the environment and health and promotes reuse and/or recycling of materials of the equip-
ment. Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.
Equipment disposal

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
®
®
®
®

Modello
M-797
M-798
Serie IM
MANUALE DI ISTRUZIONI
Ver. 1.1 2022

Pagina 20
Sommario
1. Avvertenza 21
2. Informazionisullasicurezza 21
3. Contenutodellaconfezione 22
3.1 M-797 22
3.2 M-798 23
4. Disimballaggio 24
5. Utilizzoprevisto 24
6. Simboli 24
7. Descrizionedellostrumento 25
7.1 M-797 25
7.2 M-798 26
8. Assemblaggio 27
8.1 Installareilportaltri 27
8.2 Installareiltriperuorescenza 27
8.2.1 M-797 27
8.2.1 M-797 28
8.3 Installareicoperchideiltri 28
8.4 Installarelalevadiselezionedeiltri(M-798) 28
8.5 Montaggio delll’epi-illuminatore 28
8.6 Montaggio del corpo lampada 29
8.7 Montaggio della lampada 29
9. Usodelmicroscopioinfluorescenza(luceriflessa) 31
9.1 Centraggio della lampada HBO 31
9.2 Centraggio del diaframma di campo 33
9.3 E󰀨ettideldiaframmadicampo 33
9.4 Filtriperuorescenzadisponibili 33
10. OsservazionesimultaneainContrastodiFase+Fluorescenza 34
Smaltimento 35