Integra Evolve 3011 User manual

EVOLVE Manual Pipettes Operating Instructions
900-00010-00_V06

i/i INTEGRA Biosciences AG
Declaration of Conformity
INTEGRA Biosciences Corp. – Hudson, NH, USA
declares on its own responsibility that the devices
comply with:
Zizers, April 11, 2022
Description Models
EVOLVE Manual
Pipettes
3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025,
3026, 3028, 3032, 3034, 3035, 3036, 3038, 3042, 3044, 3045
EU Regulations Scope Date effective
1907/2006
Registration, evaluation, authorisation and
restriction of chemicals (REACH)
01.06.2007
EU Standards Scope
EN 9001:2015 Quality Management
USA Regulations Scope
17 CFR Parts 240 & 249b Dodd frank “Conflict minerals”
27 CCR Parts 25102-
27001
Proposition 65: The safe drinking water and
toxic enforcement act
TSCA 40 CFR Part 751 Toxic substances control act
International Standards
ISO 8655-2 Piston pipettes
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality

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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................5
1.3 Safety notes .....................................................................................6
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................7
2.2 Overview of the EVOLVE manual pipettes.......................................7
Chapter 3 Operation
3.1 Setting the volume............................................................................8
3.2 Attaching and removing GRIPTIP pipette tips..................................8
3.3 Pipetting ...........................................................................................9
3.3.1 Aspirating and dispensing.....................................................9
3.3.2 Reverse mode.....................................................................10
3.3.3 Proper pipetting techniques ................................................11
3.4 Troubleshooting..............................................................................12
Chapter 4 Maintenance
4.1 Cleaning .........................................................................................13
4.2 Single channel lower end disassembly ..........................................13
4.2.1 Disassembly........................................................................13
4.2.2 Reassembly ........................................................................16
4.3 Sterilization.....................................................................................18
4.3.1 Autoclaving the disassembled components........................18
4.4 Servicing.........................................................................................19
4.4.1 Shipping to INTEGRA Biosciences.....................................19
4.4.2 Changing O-rings of tip fittings............................................19
4.4.3 Lubrication ..........................................................................20
4.5 Calibration ......................................................................................21
4.5.1 Materials .............................................................................21
4.5.2 Definitions ...........................................................................21
4.5.3 Test conditions and environment ........................................22
4.5.4 Pre-wetting of tips ...............................................................22
4.5.5 Leak test .............................................................................23
4.5.6 Obtaining the actual volume ...............................................24
4.5.7 Calculation of accuracy and precision ................................25
4.5.8 Adjusting EVOLVE manual pipettes ...................................26
4.6 Equipment disposal ........................................................................27

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Chapter 5 Technical data
5.1 Environmental conditions ...............................................................28
5.2 Pipette specifications......................................................................29
5.3 Z correction factors.........................................................................31
Chapter 6 Accessories and Consumables
6.1 Accessories ....................................................................................32
6.2 Consumables .................................................................................32
6.3 GRIPTIPS.......................................................................................33
6.3.1 GRIPTIPS for handheld pipettes.........................................33
6.3.2 Package options .................................................................33
6.3.3 GRIPTIP properties.............................................................33
Imprint ...................................................................................................34

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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the EVOLVE manual pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the EVOLVE manual
pipettes and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
WARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage. Follow the
instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is under the sole responsibility of the user.
EVOLVE manual pipettes are intended to be used in a laboratory for aspirating and
dispensing liquids in the volume range of 0.2–5000 µl using GRIPTIP pipette tips.

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1.3 Safety notes
EVOLVE manual pipettes comply to the recognized safety regulations and is safe to
operate. EVOLVE manual pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the EVOLVE manual pipettes must have read
and understood these operating instructions, and particularly, the safety notes, or must
have been instructed by supervisors so that safe operation of the device is guaranteed.
WARNING
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
• Do not immerse the EVOLVE manual pipettes in liquid. The fluid can
damage internal parts. Avoid pipetting of liquids whose vapors could
attack the materials PA (polyamide), POM (polyoxymethylene), FPM
(fluor-rubber), NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive
vapors could also damage metallic parts inside the device.
• Repairs may only be performed by INTEGRA Biosciences AG or by an
authorized after-sales service member.
• Parts may be replaced with original INTEGRA Biosciences parts only.
NOTE
Prolonged exposure of the EVOLVE manual pipettes to UV-light can cause
discoloration and/or yellowing of the pipette housing. However, this will not
affect the performance of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

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2 Description of the device
2.1 Scope of delivery
• EVOLVE manual pipette
• Multipurpose tool
• Color coded plunger cap
• Quick start guide
• Replacement O-rings (for 200 μl, 1000 μl and 5000 μl models)
2.2 Overview of the EVOLVE manual pipettes
1Plunger cap
2Plunger
3Tip ejector
4Finger hook
5Volume adjustment dials
6Volume window
The color of the volume indicator label matches
GRIPTIP rack insert
7Ejector sleeve
8Tip fitting
1
2
4
6
3
5
7
8

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3 Operation
3.1 Setting the volume
3.2 Attaching and removing GRIPTIP pipette tips
CAUTION
To ensure optimal performance of your EVOLVE manual pipettes always use
GRIPTIPS of correct size which have been designated for the use with the
EVOLVE manual pipettes, see “6.2 Consumables” on page 32.
The unique GRIPTIP system of the EVOLVE manual pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and provides a
perfect seal. On a multichannel pipette, all tips sit at the same height.
Attach the tips:
When loading tip(s), press the pipette into the appropriate GRIPTIP(s) until you hear and
feel a click indicating that a seal has been achieved. Once you feel the click, stop applying
pressure. When loading GRIPTIP pipette tips using a multichannel pipette, slowly rock the
pipette from one side to the other side to ensure the proper seal is achieved.
Discard your used tips:
If liquid is in the tips, empty them by pressing down the plunger (2) to the second stop
(see section 3.3). Tips are easily removed by pressing the tip ejector (3).
1) To set the desired pipetting volume press down the plunger (a)
and rotate slightly clockwise to release the volume dials.
2) Spin the adjustment dials (b) until the desired volume is shown
in the volume window.
3) Slightly rotate and slowly release the plunger (a) to set the
volume.
a
b
A rim inside the GRIPTIPS snaps over the multi-lobes and ensures
firm attachment of the tips.
A shoulder provides a positive stop to prevent over-tightening of the
tips. Without hammering, the tip is either on or off but nothing in
between.
O-ring provides forgiving and robust seal surface for the pipette tip.

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3.3 Pipetting
The plunger can be pushed down to two different stops:
For detailed recommendations for pipetting, see also “3.3.3 Proper pipetting techniques”
on page 11.
3.3.1 Aspirating and dispensing
To aspirate and dispense liquid, perform the following steps:
1) Top position: Varies depending on volume setting.
2) First stop: Fixed calibrated position for aspirating and
dispensing.
3) Second Stop: To blowout the residual liquid.
Aspirate
1) With GRIPTIPS attached, push down the plunger to the first
stop.
2) Immerse the GRIPTIPS in liquid.
3) Slowly return plunger to the top position. For higher volumes
(>200 μl), follow the liquid level by moving the pipette down.
4) Remove the GRIPTIPs from liquid.
Dispense
5) To dispense the liquid, push the plunger down to the first stop.
6) If there is any residual liquid in tip, a blowout should be
performed by pressing the plunger to the second stop.
7) Remove the GRIPTIPS from vessel and release plunger.
1
2
3

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3.3.2 Reverse mode
Viscous samples should be aspirated and dispensed at slower speeds and in reverse
pipetting mode:
Aspirate
1) Fully push down the plunger to the second stop.
2) Immerse the GRIPTIPS in the liquid, let the plunger slowly
release to the upper position and wait shortly until the liquid is
fully aspirated.
Dispense
3) To dispense, press the plunger slowly down to the first stop and
wait a few seconds to dispense the liquid.
4) Remove the GRIPTIPS from the target vessel.
5) To expel the excess liquid, press the plunger to the second
stop.