Integra Evolve 3011 User manual

EVOLVE Manual Pipettes Operating Instructions
900-00010-00_V06

i/i INTEGRA Biosciences AG
Declaration of Conformity
INTEGRA Biosciences Corp. – Hudson, NH, USA
declares on its own responsibility that the devices
comply with:
Zizers, April 11, 2022
Description Models
EVOLVE Manual
Pipettes
3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025,
3026, 3028, 3032, 3034, 3035, 3036, 3038, 3042, 3044, 3045
EU Regulations Scope Date effective
1907/2006
Registration, evaluation, authorisation and
restriction of chemicals (REACH)
01.06.2007
EU Standards Scope
EN 9001:2015 Quality Management
USA Regulations Scope
17 CFR Parts 240 & 249b Dodd frank “Conflict minerals”
27 CCR Parts 25102-
27001
Proposition 65: The safe drinking water and
toxic enforcement act
TSCA 40 CFR Part 751 Toxic substances control act
International Standards
ISO 8655-2 Piston pipettes
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality

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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................5
1.3 Safety notes .....................................................................................6
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................7
2.2 Overview of the EVOLVE manual pipettes.......................................7
Chapter 3 Operation
3.1 Setting the volume............................................................................8
3.2 Attaching and removing GRIPTIP pipette tips..................................8
3.3 Pipetting ...........................................................................................9
3.3.1 Aspirating and dispensing.....................................................9
3.3.2 Reverse mode.....................................................................10
3.3.3 Proper pipetting techniques ................................................11
3.4 Troubleshooting..............................................................................12
Chapter 4 Maintenance
4.1 Cleaning .........................................................................................13
4.2 Single channel lower end disassembly ..........................................13
4.2.1 Disassembly........................................................................13
4.2.2 Reassembly ........................................................................16
4.3 Sterilization.....................................................................................18
4.3.1 Autoclaving the disassembled components........................18
4.4 Servicing.........................................................................................19
4.4.1 Shipping to INTEGRA Biosciences.....................................19
4.4.2 Changing O-rings of tip fittings............................................19
4.4.3 Lubrication ..........................................................................20
4.5 Calibration ......................................................................................21
4.5.1 Materials .............................................................................21
4.5.2 Definitions ...........................................................................21
4.5.3 Test conditions and environment ........................................22
4.5.4 Pre-wetting of tips ...............................................................22
4.5.5 Leak test .............................................................................23
4.5.6 Obtaining the actual volume ...............................................24
4.5.7 Calculation of accuracy and precision ................................25
4.5.8 Adjusting EVOLVE manual pipettes ...................................26
4.6 Equipment disposal ........................................................................27

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Chapter 5 Technical data
5.1 Environmental conditions ...............................................................28
5.2 Pipette specifications......................................................................29
5.3 Z correction factors.........................................................................31
Chapter 6 Accessories and Consumables
6.1 Accessories ....................................................................................32
6.2 Consumables .................................................................................32
6.3 GRIPTIPS.......................................................................................33
6.3.1 GRIPTIPS for handheld pipettes.........................................33
6.3.2 Package options .................................................................33
6.3.3 GRIPTIP properties.............................................................33
Imprint ...................................................................................................34

EVOLVE manual pipettes – Operating instructions V06 Introduction
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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the EVOLVE manual pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the EVOLVE manual
pipettes and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
WARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage. Follow the
instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is under the sole responsibility of the user.
EVOLVE manual pipettes are intended to be used in a laboratory for aspirating and
dispensing liquids in the volume range of 0.2–5000 µl using GRIPTIP pipette tips.

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1.3 Safety notes
EVOLVE manual pipettes comply to the recognized safety regulations and is safe to
operate. EVOLVE manual pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the EVOLVE manual pipettes must have read
and understood these operating instructions, and particularly, the safety notes, or must
have been instructed by supervisors so that safe operation of the device is guaranteed.
WARNING
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
• Do not immerse the EVOLVE manual pipettes in liquid. The fluid can
damage internal parts. Avoid pipetting of liquids whose vapors could
attack the materials PA (polyamide), POM (polyoxymethylene), FPM
(fluor-rubber), NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive
vapors could also damage metallic parts inside the device.
• Repairs may only be performed by INTEGRA Biosciences AG or by an
authorized after-sales service member.
• Parts may be replaced with original INTEGRA Biosciences parts only.
NOTE
Prolonged exposure of the EVOLVE manual pipettes to UV-light can cause
discoloration and/or yellowing of the pipette housing. However, this will not
affect the performance of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

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2 Description of the device
2.1 Scope of delivery
• EVOLVE manual pipette
• Multipurpose tool
• Color coded plunger cap
• Quick start guide
• Replacement O-rings (for 200 μl, 1000 μl and 5000 μl models)
2.2 Overview of the EVOLVE manual pipettes
1Plunger cap
2Plunger
3Tip ejector
4Finger hook
5Volume adjustment dials
6Volume window
The color of the volume indicator label matches
GRIPTIP rack insert
7Ejector sleeve
8Tip fitting
1
2
4
6
3
5
7
8

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3 Operation
3.1 Setting the volume
3.2 Attaching and removing GRIPTIP pipette tips
CAUTION
To ensure optimal performance of your EVOLVE manual pipettes always use
GRIPTIPS of correct size which have been designated for the use with the
EVOLVE manual pipettes, see “6.2 Consumables” on page 32.
The unique GRIPTIP system of the EVOLVE manual pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and provides a
perfect seal. On a multichannel pipette, all tips sit at the same height.
Attach the tips:
When loading tip(s), press the pipette into the appropriate GRIPTIP(s) until you hear and
feel a click indicating that a seal has been achieved. Once you feel the click, stop applying
pressure. When loading GRIPTIP pipette tips using a multichannel pipette, slowly rock the
pipette from one side to the other side to ensure the proper seal is achieved.
Discard your used tips:
If liquid is in the tips, empty them by pressing down the plunger (2) to the second stop
(see section 3.3). Tips are easily removed by pressing the tip ejector (3).
1) To set the desired pipetting volume press down the plunger (a)
and rotate slightly clockwise to release the volume dials.
2) Spin the adjustment dials (b) until the desired volume is shown
in the volume window.
3) Slightly rotate and slowly release the plunger (a) to set the
volume.
a
b
A rim inside the GRIPTIPS snaps over the multi-lobes and ensures
firm attachment of the tips.
A shoulder provides a positive stop to prevent over-tightening of the
tips. Without hammering, the tip is either on or off but nothing in
between.
O-ring provides forgiving and robust seal surface for the pipette tip.

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3.3 Pipetting
The plunger can be pushed down to two different stops:
For detailed recommendations for pipetting, see also “3.3.3 Proper pipetting techniques”
on page 11.
3.3.1 Aspirating and dispensing
To aspirate and dispense liquid, perform the following steps:
1) Top position: Varies depending on volume setting.
2) First stop: Fixed calibrated position for aspirating and
dispensing.
3) Second Stop: To blowout the residual liquid.
Aspirate
1) With GRIPTIPS attached, push down the plunger to the first
stop.
2) Immerse the GRIPTIPS in liquid.
3) Slowly return plunger to the top position. For higher volumes
(>200 μl), follow the liquid level by moving the pipette down.
4) Remove the GRIPTIPs from liquid.
Dispense
5) To dispense the liquid, push the plunger down to the first stop.
6) If there is any residual liquid in tip, a blowout should be
performed by pressing the plunger to the second stop.
7) Remove the GRIPTIPS from vessel and release plunger.
1
2
3

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3.3.2 Reverse mode
Viscous samples should be aspirated and dispensed at slower speeds and in reverse
pipetting mode:
Aspirate
1) Fully push down the plunger to the second stop.
2) Immerse the GRIPTIPS in the liquid, let the plunger slowly
release to the upper position and wait shortly until the liquid is
fully aspirated.
Dispense
3) To dispense, press the plunger slowly down to the first stop and
wait a few seconds to dispense the liquid.
4) Remove the GRIPTIPS from the target vessel.
5) To expel the excess liquid, press the plunger to the second
stop.

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3.3.3 Proper pipetting techniques
INTEGRA Biosciences recommends the following techniques for enhancing pipetting
results. These techniques are consistent with ISO standard 8655-2.
• It is best to immerse the GRIPTIPS just below the surface of the liquid (2–3 mm) to
allow the desired volume to be aspirated. If the tip is immersed too deep, the risk of
carry-over is increased due to liquid drops clinging to the outer surface of the tip.
• Press and release the plunger with consistent speed and smoothness.
• Always pre-wet GRIPTIPS. After loading tips onto your pipette, aspirate and dispense
the full volume 2-3 times to coat the inside of pipet tips. Pre-wetting ensures that the
liquid and air inside the tips are at equal temperature and the dead air space is humid-
ified. Neglecting to perform a pre-wet can result in a smaller delivery volume in the first
few dispenses.
• EVOLVE manual pipettes are air displacement pipettes. To properly dispense liquids,
ensure that the pipette tip is at a 0–20° angle. After each dispense, you must touch
GRIPTIPS against the vessel wall or liquid. This process is referred to as “touching
off” or “tip touch” and prevents liquid from clinging to the pipette tips.
• Air displacement pipettes show the best performance between 35% and 100% of the
nominal volume.
• Viscous samples should be aspirated and dispensed at slow speeds to ensure accu-
rate pipetting. In addition, pipetting should be performed in a reverse mode.
• For pipetting liquids with high vapor pressures (such as methanol or ethanol), use rel-
atively fast pipetting speeds and avoid prolonged pauses after aspiration.
• Calibrate based on fluid type. EVOLVE manual pipettes are tested and calibrated at
the factory for use with distilled water at room temperature. It may be necessary to re-
calibrate your EVOLVE manual pipettes if the liquid to be used has different physical
properties (specific gravity and vapor pressure) than water.
WARNING
Avoid pipetting for extended periods. To minimize the risk of repetitive strain
injury, include pauses of several minutes.
Use filtered GRIPTIPS to pipette corrosive or biohazardous liquids. The filter
prevents vapors and aerosols from corroding or contaminating the cylinder
assembly.

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3.4 Troubleshooting
Problem Probable cause Remedy
Leaking. • Tip incorrectly attached.
• Foreign particles between tip
and tip fitting.
• Damaged colored O-ring.
• Attach a new tip.
• Clean tip fitting. Attach new tips
• Change the O-ring, see 4.4.2.
• If leak persists, contact service.
Dispense results
are inaccurate.
• Unsuitable calibration for
liquid class.
• Improper pipetting
techniques.
• Recalibrate with the liquids in
question.
• Adjust aspiration and dispense
speed depending on liquid:
- High viscosity liquids may
require calibration.
- High vapor pressure liquids
may require pre-wetting.
- Refer to proper pipetting
techniques section 3.3.3
Not dispensing/
aspirating.
• Piston stuck or not
connected.
• Internal seal is damaged.
• Contact service.
Droplets on the
tips.
• Temperature of liquid differs
from that of air inside the tips.
• Liquid of low viscosity and
high vapor pressure.
• Touch off was not performed.
• Pre-wet tips up to 3 times.
• Increase dispensing speed.
• Perform a touch-off.

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4 Maintenance
4.1 Cleaning
The materials used on the exterior of the EVOLVE manual pipettes support regular
cleaning intervals. Clean the external components with a lint-free cloth lightly soaked with
mild soap solution in distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never
use acetone or other solvents.
WARNING
• DO NOT immerse the entire pipette into the cleaning solution.
• DO NOT spray cleaning solution directly onto the exterior body of the
pipette.
If the pipette is exposed to corrosive vapors, e.g. from concentrated acids, or if liquid
enters the lower pipetting end, disassemble the lower part of the pipette and clean the
internal parts with distilled water or with a 70 % dilution of Isopropyl or Ethanol. Follow the
instructions below for disassembly.
4.2 Single channel lower end disassembly
4.2.1 Disassembly
Models of all sizes:
1) Before you separate the lower part from a single
channel pipette, press down the plunger and rotate
it clockwise into lock position, see 3.1.
2) Unscrew the cylinder assembly (a) clockwise and
gently slide it down from the pipette body.
This exposes the piston assembly (b, models 2–
100 µl) or piston with mounted cup seal (models
200–1000 µl).
a
b

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Models 2 –10 µl, 20 µl:
3) Slide the following components from the piston (h):
•O-ring (black, g) and seal/backer (f) assembly
•Pressure sleeve (e)
•Retainer spring (d)
•Retainer sleeve (c)
Separate the piston (h) from the upper part of the
pipette. It is held in place by a small magnet.
If the O-ring and seal/backer assembly (f) are still
stuck in the Cylinder after the piston is removed, tap
the top face of the Cylinder on the table.
Set these components aside or place them in an
autoclave pouch.
2–10 µl 20 µl
Model 100 µl:
3) Slide the following components from the piston (h):
•O-ring (black, g) and seal (white, f) assembly
•Retainer spring (d)
•Retainer sleeve (c)
Separate the piston (h) from the upper part of the
pipette. It is held in place by a small magnet.
Set these components aside or place them in an
autoclave pouch.
100 µl
200 µl
1000 µl
Models 200 µl and 1000 µl:
3) Separate the piston assembly from the upper part
of the pipette. It is held in place by a magnet.
Slide finger nail under the smaller diameter of the
cup seal (m) and push it off the Piston.
Set these components aside or place them in an
autoclave pouch.
f
c
d
h
e
g
f
c
d
h
e
g
f
c
d
hg
m
h
m
h
m

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Cylinder assemblies (a):
Models of all sizes:
5) Set all components of the lower part aside or place them in an autoclave pouch.
Model 5000 µl:
3) Separate the piston (h) from the upper part of the
pipette. Release the magnetic holder by bending the
cylinder assembly (a) 90 degrees to the side.
5000 µl
Models 2–1000 µl:
4) With the cylinder assembly (a) in hand, turn
counterclockwise to remove the black snap ring (i).
Pull out the black cylinder (k) from the white
sleeve (l).
Remove the ejector spring (j).
Model 5000 µl:
4) Put your fingernails into the gap of the white sleeve
(l) and pull off the sleeve cap (n). With the black
cylinder (k) in hand unscrew the upper part.
Remove the piston (h) with mounted cup seal.
Remove the ejector spring (j).
h
a
k
i
l
j
h
l
n
k
j

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4.2.2 Reassembly
Before reassembling the pipette, check each component for lint or dust particles. It is
recommended to replace the O-ring and seal or cup seal. Lightly lubricate the Piston, O-
Rings and Seals (see “4.4.3 Lubrication” on page 20).
Models 2 µl– 1000 µl:
Models 2 µl– 100 µl:
See “4.2.1 Disassembly” on page 13 for visual representations of all sized lower ends.
Models 200 µl, 1000 µl:
1) Slide the black cylinder (k) into the white sleeve (l).
Rotate it until it falls into position. Press the cylinder
down, and make sure it is sitting straight in the
sleeve.
2) Slide the ejector spring (j) onto the top of the
cylinder (k).
3) Position the black snap ring (i) over the cylinder (k)
at the top of the sleeve (l). Turn the sleeve
clockwise until the snap ring snaps into place and
secures the cylinder in the cylinder assembly.
4) Slide the retainer spring (d), the pressure sleeve
(e; 2, 10, 20 µl only), the
seal (f) and lightly lubri-
cated
O-ring (g) onto the piston (h). Be sure the
black O-ring is securely slid into the white seal/
backer (f; 2–10 µl and 100 µl only).
5) With the
O-ring (g) and seal assembly at the very
end, gently place piston assembly into the cylinder
assembly. Let the piston assembly fall into place
without pushing it in, so as not to damage the thin
pistons.
6) Slide the large retainer sleeve (c) into the cylinder
assembly. The extended lip faces the upper part of
the pipette. The sleeve should be resting on the
ejector spring.
4) Attach cup seal (m) to the piston (h). Insert the
piston into the greased cylinder assembly.
lkj
i
k
l
d
h
e
f
g
g
c
h
m

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Model 5000 µl:
Models of all sizes:
7) Position the piston at the small magnet to connect the piston to the upper part of the
pipette.
8) Screw the cylinder assembly (a) counterclockwise to attach it to the body of the
pipette.
Perform a leak test (see “4.5.5 Leak test” on page 23) and validate pipetting volumes after
reassembly.
1) Insert the cup seal of the piston (h) into the top of
greased cylinder‘s lower part (k). Work back and
forth to spread the lube around the cylinder walls.
Screw on the upper part of cylinder (k).
2) Align the slots on the cylinder (k) with the bosses
inside the sleeve (l) and slide the cylinder assembly
into place.
3) Ensure that the four noses (o) of the sleeve cap
point to the openings (p) of the sleeve.
Re-attach the sleeve cap (n).
kk
h
k
l
o
p
n

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4.3 Sterilization
If the surface of the EVOLVE manual pipettes have been in contact with biohazardous
material, they must be decontaminated in accordance to good laboratory practice. Wipe
the clean surface with a lint-free cloth, lightly soaked e. g. with the following disinfectants:
• Ethanol 70 %
• Microcide SQ 1:64
• Glutaraldehyde solution 4%
• Virkon solution 1-3%
Follow the instructions provided with the disinfectants.
The device may be decontaminated with H2O2gas (maximal concentration 35 %) for 60
minutes.
It is not recommended to autoclave the EVOLVE manual pipettes. If autoclaving is
required, only the lower assembly of the EVOLVE single channel pipettes can be
autoclaved.
WARNING
Service is required after autoclaving the EVOLVE manual pipettes!
Do not autoclave the entire unit. Multichannel pipettes CANNOT be
autoclaved, they might be damaged!
As-found calibration (measurement report, indicating “before” data) is not
possible after autoclaving!
4.3.1 Autoclaving the disassembled components
Place the disassembled components (see 4.2.1) into steam autoclave in an autoclave
pouch.
You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes.
CAUTION
Service is required after autoclaving the EVOLVE Manual Pipettes!

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4.4 Servicing
4.4.1 Shipping to INTEGRA Biosciences
For any service or repairs, please contact your local service technician.
WARNING
If working with infectious materials, e. g. human pathogens, the EVOLVE
manual pipettes need to be decontaminated before sending to service and
the declaration on the absence of health hazards must be signed. This is
necessary to protect service personnel.
4.4.2 Changing O-rings of tip fittings
200 µl, 1000 µl and 5000 µl EVOLVE manual pipettes feature tip fittings with colored O-
rings. This O-ring is used to seal against the inside wall of GRIPTIPS and provides a
robust seal.
O-rings are made of durable silicone. If necessary, e.g. in case of a leakage due to
damaged O-ring, you can replace these O-rings. A set of spare O-rings can be ordered
separately, see “6.2 Consumables” on page 32.
WARNING
Avoid mechanical damage of the tip fittings.
For 200 µl or 1000 µl pipettes, slide the multipurpose tool sideways
onto the tip fitting until the O-ring (a) builds a loop. Remove the O-
ring with plastic tweezers.
For 5000 µl pipettes, simply use plastic tweezers to remove the O-
ring.
Slide a new O-ring over the tip fitting (b).
a
a
b

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4.4.3 Lubrication
The internal seals and O-rings are subject to wear. An undamaged thin lubricant film is
important to keep the seals tight. The lubricant recommended depends on the EVOLVE
pipette size, see “6.2 Consumables” on page 32:
Single channel 2 µl – 10 µl models:
Use the Fluorocarbon Gel (#100-00136-50) to lightly lubricate the
piston (h) without components (f, g) slid onto. Put a drop of grease
onto your fingers, lubricate the black O-ring (g) and slide it over the
white seal (f).
Single channel 20 µl and 100 µl model:
Use the Kluberalfa Lube (#500-00182-00) to lightly lubricate the
piston (h) without components (f, g) slid onto. Lightly lubricate outer
ring of the O-ring seal (g).
Single channel 200 µl and 1000 µl models:
Use the Kluberalfa Lube (#500-00182-00) to lightly lubricate the
outer ring of the Cup Seal (m). Do not lubricate the bottom of Cup
Seals.
Single channel 5000 µl model:
Use the Kluberalfa Lube (#500-00182-00) to lightly lubricate the
outer ring of the Cup Seal (m) and O-Ring (o). Do not lubricate the
bottom of Cup Seals.
g
f
h
g
f
h
m
m
m
o