Olympus DP2-SAL User manual

Optical Microscope Accessory
INSTRUCTIONS
This instruction manual is for the standalone connection kit for Olympus microscope
digital camera.
To obtain optimum performance of this system and to ensure the safety, study this
manual thoroughly before operating the system and keep it on hand during operation
of this system.
Keep this instruction manual in a safe place.
For details of products included in the configuration of this system, see page 8 of this
instruction manual.
Standalone connection kit
DP2-SAL

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
CALIFORNIA USA ONLY
This product uses a Lithium Battery which contains Perchlorate Material -special handling may apply, See
www.dtsc.ca.gov/hazardouswaste/perchlorate.
In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.
This product is applied with the requirements of standard IEC/EN61326-1 concerning electromagnetic
compatibility.
- Immunity Applied to industrial and basic environment requirements.
For Korea only
B급 기기 (가정용 방송통신기자재)
이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을
목적으로 하며, 모든 지역에서 사용할 수 있습니다.

Contents
Introduction........................................................................................................................................................1
Safety precautions.......................................................................................................................................2
Handling precautions................................................................................................................................4
1 System diagram........................................................................................................................................8
2 Nomenclature of respective portions....................................................................................9
2-1 Hardware controls.............................................................................................................................................. 9
2-2 Control window .................................................................................................................................................11
3 Assembly.......................................................................................................................................................16
1Installing the control box D2-CB...................................................................................................................................16
2Connecting the interface cable..................................................................................................................................... 16
3Connecting the display cable......................................................................................................................................... 17
4Connecting the AC adapter..............................................................................................................................................18
5Connecting USB devices....................................................................................................................................................20
6LAN Connection ..........................................................................................................................................................................21
4 Acquisition ..................................................................................................................................................22
4-1 Basic operations...............................................................................................................................................22
1Start and exit system..............................................................................................................................................................23
2Selecting exposure mode..................................................................................................................................................25
3Exposure compensation .....................................................................................................................................................25
4Acquiring still images.............................................................................................................................................................26
5ROI White Balance....................................................................................................................................................................27
6Setting AE lock.............................................................................................................................................................................28
7Show/Hide control window ..............................................................................................................................................28
8Shutdown and Sleep ..............................................................................................................................................................28

4-2 Basic settings......................................................................................................................................................29
1Setting live image resolution ...........................................................................................................................................29
2Setting still image resolution............................................................................................................................................30
3Setting still image format ....................................................................................................................................................31
4Setting ISO sensitivity.............................................................................................................................................................32
5Setting white balance (WB) mode..............................................................................................................................32
6Setting metering area.............................................................................................................................................................33
7Adjusting contrast ....................................................................................................................................................................33
8Setting image color..................................................................................................................................................................34
9Setting sharpness......................................................................................................................................................................34
4-3 Advanced operations...................................................................................................................................35
1Manual exposure acquisition..........................................................................................................................................35
2Recording Movie.........................................................................................................................................................................36
3Series Snap.....................................................................................................................................................................................37
4Pause/Resume Live.................................................................................................................................................................37
5Display zoom/scroll..................................................................................................................................................................38
6Selecting objective magnification...............................................................................................................................38
7Selecting Zoom magnification / Magnification Changer.........................................................39
8Selecting Mirror unit ......................................................................................................................................................39
4-4 Advanced settings..........................................................................................................................................40
1Setting AE area.............................................................................................................................................................................40
2Shading correction ...................................................................................................................................................................40
3Setting sound of movie.........................................................................................................................................................42
4Setting image orientation....................................................................................................................................................43
5Setting focus indicator...........................................................................................................................................................43
6Setting scale display...............................................................................................................................................................45
7Setting save folders and file names..........................................................................................................................46
8Auto save folders and auto file names...................................................................................................................48

DP2-SAL
9Media setup....................................................................................................................................................................................49
10 Reset .....................................................................................................................................................................................................50
11 Display Off setting......................................................................................................................................................................53
12 Camera Calibration ..................................................................................................................................................................54
5 Play.....................................................................................................................................................................57
5-1 Basic operations...............................................................................................................................................57
1Selecting play images ...........................................................................................................................................................57
2Display zoom/scroll..................................................................................................................................................................58
3Playing movies..............................................................................................................................................................................58
4Extracting frames from movies......................................................................................................................................59
5Protect play image....................................................................................................................................................................59
6Deleting images ..........................................................................................................................................................................60
5-2 Advanced operations................................................................................................................................... 61
12 Window .........................................................................................................................................................................................61
2Display Info tab.............................................................................................................................................................................61
6 Measurement ..........................................................................................................................................62
1Measurement functions........................................................................................................................................................62
2Outputting measurement results in CSV file......................................................................................................75
3Displaying cross lines............................................................................................................................................................75
4Setting display target of scales and cross lines ............................................................................................76
7 Initial Settings............................................................................................................................................77
1Setting the language...............................................................................................................................................................78
2Setting the microscope ........................................................................................................................................................78
3Setting Date/Time......................................................................................................................................................................82
4Setting time zone .......................................................................................................................................................................82
5Setting display resolution ..................................................................................................................................................83
6Setting network............................................................................................................................................................................84

7Installing special device driver.......................................................................................................................................86
8Canceling password lock of USB recording media....................................................................................88
8 Functions linked with microscope.........................................................................................89
8-1 Application conditions.................................................................................................................................89
8-2 Connecting procedures............................................................................................................................. 90
8-3 Operating procedures .................................................................................................................................92
9 Warning list .................................................................................................................................................93
10 Specifications.........................................................................................................................................94
11 Troubleshooting....................................................................................................................................96

DP2-SAL
1
Introduction
Contents in this instruction manual
This instruction manual covers only the contents concerning the standalone connection kit DP2-SAL. For procedures to
use the microscope digital camera DP22/DP27, refer to the instruction manual of the microscope digital camera DP22/
DP27.
Trademarks
Microsoft and Windows are registered trademarks of Microsoft Corporation in U.S. All brand names or product names
described in this instruction manual are trademarks or registered trademarks of relevant owners.
Software license agreement
If you use this product, you shall be deemed to have consented to the terms and conditions in “OLYMPUS END-USER
LICENSE AGREEMENT”. “OLYMPUS END-USER LICENSE AGREEMENT” is included in F: EULA of the product. Please
read it carefully before using the product.
Open Source Software
A part of this product contains the programs or codes of the open source software or the software disclosed intentionally
(hereinafter called “OSS”). The programs and codes of OSS included in this product shall comply with license conditions
applied to the said OSS. Also, any part of this agreement shall not restrict, limit or affect the rights owned by customers
or conditions complied by customers based on the license conditions of the said OSS. The information of the programs
and codes of OSS included in this product is contained in F: license of this product.
The standalone connection kit DP2-SAL is a product to use the microscope digital camera DP22/DP27 in standalone
mode.
Connecting the Olympus microscope digital camera DP22/DP27 to this product allows you to use the microscope
digital camera DP22/DP27 in standalone mode.

2
Safety precautions
CAUTION - Electric safety -
Always use the AC adapter and the power cord provided by Olympus.
If the proper AC adapter and the power cord are not used, the electric safety and the EMC (Electro-Magnetic
Compatibility) performance of the device cannot be assured.
Always connect the ground terminal.
Connect the ground terminal of the power cord and that of the power outlet. If the device is not grounded, our
intended electric safety and EMC performance of the device can not be assured.
Do not use the device in close proximity of the sources of strong electromagnetic radiation.
Proper operation may be interfered. The electromagnetic environment should be evaluated prior to operation of this
device.
Remove the power cord in case of emergency.
In case of emergency, be sure to unplug the power cord from the power cord connector on the product or from the
wall power outlet. Install the product at the location where you can reach the power cord connector or the wall power
outlet at hand to remove the power cord quickly.
Be sure to turn OFF the power of the device before connecting /disconnecting cables and units.
Insert the cables completely to the end of the connector.
Before pressing the main switch, be sure to make sure that the cable which connects the control box D2-CB and
each unit is inserted completely to the end of the connector.
If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
product may also be damaged. Always use the equipment according to this instruction manual.
The following symbols are used in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the equipment or other property.
} : Indicates commentary (for ease of operation and maintenance).
CAUTION - Protection for electric shock -
Do not insert any tools or metal fragments in the air vents of the device.
Doing so could cause electric shock or failure of the product.
Keep the power cord and cables well away from devices generating heat.
If the power cord and cables contact a hot area, they could melt and cause electric shock.

DP2-SAL
3
CAUTION - Protection for burns -
Do not keep touching the AC adapter, the camera head or the control box D2-CB for long period of
time.
The AC adapter, the camera head or the control box D2-CB generate heat after long hours of use. Be careful not to
allow these devices to touch your skin for long period of time to avoid the low temperature burns. For details of USB
devices to be connected to the control box D2-CB such as USB memory, etc., follow the instructions described in
instruction manual of each device.
The following symbols are placed on the product.
Study the meaning of the symbols and always use the equipment in the safest possible manner.
Symbol Meaning
Indicates a non-specific general hazard. Follow the cautions given after this symbol
or in the instruction manual.
Caution labels
Cautions are displayed at the areas where special cautions are required when in use and operation. Be sure to follow
the instructions.
Caution label
position Top surface of the control box D2-CB
When caution labels are dirty or peeled off, contact Olympus for replacement or inquiries.
CAUTION - Safety symbols -
3

4
1. This equipment is a precision instrument. Handle it with care and avoid subjecting it to a sudden or severe impact.
2. Never disassemble any part of the product. Otherwise, failure could be caused.
3. Do not use this equipment in areas where it may be subjected to direct sunlight, high temperature and humidity,
dust or vibrations. For use environment, refer to 10 Specifications on p.94.
4. The cables are vulnerable to bend or twist. Do not apply excessive force. When connecting cables, make sure of
the orientation to insert them.
5. If cables are connected incorrectly, it may cause a failure of the product. Make sure that the cables are connected
to respective units correctly before operating the product.
Image data
1. The recorded image data may be lost (destroyed) in the following cases. Olympus is not liable for the loss
(destruction) of recorded data.
· When the user or a third party used the recording media such as USB memory, etc. incorrectly.
· When the user or a third party carried out a repair to the product.
· When the recording media such as USB memory, etc. is affected by static or electric noise.
· When following actions were taken while Windows®or the control box is recording to the recording media
such as USB memory, etc. or while deleting (initializing).
· Disconnect the recording media or cables, etc.
· Shutdown the system.
· Set the main switch to OFF.
· Unplug the AC adapter.
· Unplug the power cord.
· When the data have been kept saved in the recording media such as USB memory beyond data storage life
(approx. from one to several years).
· When the product failed.
2. If following phenomena occur even though the camera is working properly, images cannot be restored and the
damages are not compensated.
· The image is abnormal.
· File properties such as file names, file time, etc. are abnormal.
· The image was lost.
} In general, as there is a data storage lifetime in the recording media, such as USB memory, HDD, CD-R, DVD-R, etc.,
the saved files may be lost after several years.
} The image data may be lost (corrupted) unexpectedly, so make frequent backups of the data you acquired.
OLYMPUS shall have no liability for any damage (including compensation for the corrupted image data) from the
use or incapable use of this product.
} Make sure that there is no problem when acquiring the subjects before acquisition. Even though the problem may
occur by the acquired images, Olympus is not liable for these problems.
Cautions in use
This product is intended to be used as an ancillary component for use with Olympus DP22 and DP27 cameras for
capture and storage of digital images, but not for clinical diagnostic purposes.
Intended use
Handling precautions

DP2-SAL
5
Guarantee of operation
If the following has been done, Olympus does not guarantee the operation of this product.
· Writing to the system disk (Windows®OS)
· Installing the software not pre-installed into this product, for example, antivirus software
Computer virus note
To prevent from computer viruses, DP2-SAL does not allow the unauthorized writing in the system area of the
control box D2-CB.
However, it cannot completely prevent the infection of computer viruses that evolve on a daily basis. To prevent the
contamination of computer viruses to the control box D2-CB, take following actions.
· For the recording media connected to DP2-SAL such as USB memory, etc., check viruses in advance. (DP2-
SAL does not have a function to check viruses. Use your PC to check viruses.)
· Make sure that there is no computer virus in the network or PC to which DP2-SAL is connected.
· If by any chance, the computer virus is mixed or if there is a suspicion of contamination, turn OFF the main
switch of the control box D2-CB and unplug the power cord from the outlet. By doing this, the RAM memory
is cleared and the foreign file is deleted.
· If malfunctions or infectious symptoms are still noticed even though the above actions were taken, contact
Olympus.
Olympus is not liable for malfunctions of DP22/DP27 or defects and damages to your PC or network environments
caused by the computer virus.
Battery life
The lithium ion coin battery (CR2032) to maintain the calendar function is built in the control box D2-CB. The
battery lifetime is between 1 and 6 years. (If the AC adapter is connected to the control box D2-CB, the battery
consumption is reduced. Therefore, the lifetime differs.)
The camera can be used even though the battery runs out, but the calendar is reset each time when the AC
adapter is removed.
If you need to replace the battery, contact Olympus.
Control box (D2-CB) fan
The control box D2-CB is equipped with a ventilation fan. Do not close the vent. Install the control box D2-CB away
from the obstacles such as walls, etc. by 10 cm or more to get the sufficient air.
Using the control box keeping the vent closed will cause malfunctions or failures. If any foreign substance is
entered from the vent, the fan may be damaged.
The number of rotation of the fan of control box D2-CB varies depending on the temperature within the system.
Therefore, the fan may make larger sound in the following cases, but they are not failures.
· When the temperature of the ambient environment is high, such as in summer time, etc.
· When the temperature of the system is high after long hours of using.
· Approximately 10 seconds at the beginning of startup

6
Restrictions in use
} Please also confirm the restrictions described in the instruction manual of the microscope digital camera you will
use.
1. The image of dark specimen under the reflected fluorescence observation or the darkfield observation (DP27:
Specimen that needs the exposure of 1/2 second or more at ISO100 equivalent. DP22: Specimen that needs
the exposure of 1/2 second or more at ISO200 equivalent) cannot be acquired. When acquiring the image of
the particularly dark specimen, the lack of pixels caused by the cosmic ray or the constant bright points may be
noticeable, but they are not things by the failure of the equipment.
2. Specimens with intensity distribution that is not suitable for the metering require using spot metering or exposure
compensation.
3. When digital zoom is used for enlarging the image during focusing, roughness of the image may become
noticeable with certain specimens.
4. If the brightness or the color of the left half of the live image is significantly different from those of the right half,
calibrate the camera. For details, refer to “Camera Calibration (P.54)“.
5. When attaching/detaching USB equipments or when accessing USB equipments, the frame rate may be
decreased or the image may be blurred temporarily.
6. The maximum frame rate according to the resolution of the live image is shown in the table below. The maximum
frame rate is given when the exposure time is as shown in the table.
DP22-CU:
Resolution Exposure time (sec) Frame rate (fps)
1920 x 1440 1/27 25
960 x 720 (Draft) 1/27 25
960 x 720 (Binning) 1/27 25
DP27-CU:
Resolution Exposure time (sec) Frame rate (fps)
2448 x 1920 1/16 15
1224 x 960 (Draft) 1/33 30
1224 x 960 (Binning) 1/33 30
7. If the scale imprinting function or the measuring function is selected, the time to acquire the image is longer than
normal.
8. When observing a specimen which contains little white area, the traceability of auto white balance is deteriorated.
9. "Format of recording media" usable with the control box D2-CB is FAT or FAT32. Other file systems are not
supported. When using the recording media in an unsupported format, format it to FAT or FAT32 by using PC.
10. If you use the USB memory equipped with special functions including security function or password lock function,
etc., enter the password using Windows®Explorer. For details, refer to "Canceling password lock of USB recording
media (P.88)".
Also, when connecting the USB equipped with security function, the destination to save files must be set manually.
For details, refer to "Setting save folders (P.46)"
11. When viewing the acquired images saved in the recording media in NTFS format such as USB-HDD (external hard
disk for connecting USB), etc. with PC, the acquisition date/time may not be correct. Set the same time zone to
DP2-SAL and PC, or format the recording media such as USB-HDD, etc. in FAT32 format.
System compatibility

DP2-SAL
7
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away
from open flames or potential sources of electrical sparks – for example, electrical equipment that is
being switched on or off, which could cause ignition of a fire.
Also remember to always use absolute alcohol only in a well-ventilated room.
1. Use a diluted neutral detergent to clean the parts. Moisten a soft cloth with diluted neutral detergent and wipe the
surfaces of the parts.
Do not use organic solvents to clean the parts since it deteriorates painted surfaces or plastic parts.
2. When disposing of this product, be sure to follow the regulations and rules of your local government. Particularly,
note that the lithium ion coin battery (CR2032) is built in the control box D2-CB. For any inquiry, contact Olympus.
3. When smoking the room for cleaning, etc., move the DP2-SAL to a place not exposed to smoke.
4. Be careful about generation of dew condensation, as this may cause failure of the product. Dew condensation is
the phenomenon that the vapor in the air contacts the surface of a metallic plate, etc. and condensates to water
drops. When the temperature suddenly changes, the dew condensation may be generated, for example when the
control box D2-CB is suddenly brought from cold place to warm place.
CAUTION
CAUTION
Maintenance and storage
12. Depending on the recording media’s speed to write images, etc., the frame rate to record movies may be reduced
or frames may be jumped. Connect the recording media (HDD/SSD) applicable for USB 3.0 to the USB 3.0
connector of the control box D2-CB for acquiring movies. If there is not sufficient space in the recording media
(HDD/SSD), frames may be jumped frequently when you start recording the movie. If the frames are jumped, the
movie is played as if fast-forwarded.
Devices connected to the control box.
1. USB devices other than described below do not work even though they are connected to the control box D2-CB.
Connectable USB device : USB recording media such as USB memory, etc. USB mouse, USB keyboard
When connecting equipment that need special drivers or equipment that need the Windows®Explorer function for
operations, refer to "Installing special device driver (P.86)".
2. Requirements for network connection are described below.
Applicable PC : DOS/V AT compatible
Applicable OS : Microsoft®Windows®8 (32-bit / 64-bit), Microsoft®Windows®7 (32-bit / 64-bit)
Standard : In accordance with IEEE802.3 (10BASE-T Ethernet), IEEE802.3u (100BASE-TX Fast Ethernet)
Applicable protocol : In accordance with TCP/IP (IPv6 is not supported yet.)
Connector shape : RJ-45 type 8-port connector
Transmission speed : 10Mbps (10BASE-T Ethernet) / 100Mbps (100BASE-TX Fast Ethernet)
Even though the above mentioned connection requirements are satisfied, it does not necessarily guarantee the
connection and operations in all network environments.
Display in use
Display available for full color view of 1024 x 768 or better

8
1System diagram
If the display is connected via a display switcher, a malfunction may be caused. Do not use a display
switcher.
} The image color setup matching the microscope is required for faithful color reproduction. For details, refer to p.34.
} Consult Olympus for the compatible microscope and camera adapter.
} Do not use the interface cable for the other purpose than intended use.
CAUTION
Camera adapter
Interface cable
USB cable for touch panel
USB memory
AC adapter
Control box
Power cord
Display cable
LAN cable To network Display
Keyboard
Mouse
DP22-CU
DP27-CU
Camera head
DP2-SAL
D2-CB
Control box for
microscope system
Other USB devices

DP2-SAL
9
2Nomenclature of respective portions
Interface cable
Control box D2-CB
Front
Back
2-1 Hardware controls
USB connector (USB2.0)
USB connector (USB2.0)
CAMERA connector
(USB3.0 connector)
USB3.0 connector
DC input connector
Line output connector
Microphone input connector
Main switch
Display connector
DVI-I/-D/-A
Camera head
Control box
D2-CB
RS-232C connector
For connecting control box
(U-CBS, etc.) (P.90)
LED
Cable holderLAN cable connector

10
Upright stabilizer
AC adapter
Stabilizer
With 2 clamp screws
Input connector
Output plug

DP2-SAL
11
2-2 Control window
Turn ON the main switch of the control box D2-CB to start the camera control software. The camera control software
is displayed in the upper right area of the display window. In the Control window, Controller tab, Play tab, Info tab, and
Menu tab are shown.
Controller tab
AE lock (P.28)
Series snap (P.37)
Pause (P.37)
Exposure time
Shutdown (p.23,28)
Sleep (p.28)
Close (P.28)
Exposure compensation (P.25,35)
Measure (P.62)
Movie (P.36)
Snap (P.26)
White balance (P.27)
Zoom (P.38)
AE (P.25,35)
Contrast (P.33)
Objective magnification (P.38)
Zoom/Mag. Changer (P.39)
The area framed by
the dotted line is
shown or hidden by
the
button.
The [Controller] tab is used mainly for acquiring images.
There are buttons which change the setting status with the same button. The setting status are described below.
Button color Status
Blue The settings displayed on the button are enabled or ON.
Gray The settings displayed on the button are disabled or OFF.
Status when the button is pressed
Mirror units (P.39)

12
Info tab
Exposure time
Exposure mode
White balance
Image orientation
AE area
Monitor resolution
Folder name
File name
Image color
Burn result into image
Sharpness
Save results
Adapter magnification
Objective
Sound of movie
Time*
Shading correction
Zoom/Mag. Changer
Still image resolution
Still image format
Exposure compensation
Movie resolution
AE lock
Live image resolution
Zoom
Number of remaining
images
ISO sensitivity
Cross hair
Contrast
Scale
The [Info] tab is used for confirming the current settings or image properties.
The area framed by
the dotted line is
shown or hidden by
the
button.
Play tab
2 Window (P.61)
Erase (P.60)
Protect (P.59)
Measure (P.62)
Thumbnail (P.57)
Playing movies (P.58)
Zoom (P.58)
The [Play] tab is used mainly for playing images.
* If you change from the [Controller] tab to the [Info] tab, [Time] shows the current time. If you change from the
[Play] tab to the [Info] tab, [Time] shows the acquisition time of the image selected in the [Play] tab.
** Displayed if the applicable product is combined.
Mirror unit**
MIX light intensity**
MIX light position**
Reflected light intensity**
Transmitted light intensity**

DP2-SAL
13
AE area (P.33)
ISO sensitivity (P.32)
Display AE area (P.33)
Sharpness (P.34)
White balance (P.32)
Image color (P.34)
Shading correction (P.40)
The [Menu] tab is used for changing the settings.
In [Menu] tab, there are [Camera control] tab, [Quality] tab, [Resolution] tab, [Display] tab, [Maintenance] tab and Setting tab.
Camera control tab
Quality tab
Menu tab

14
Live image resolution (P.29)
Image orientation (P.43)
Still image resolution (P.30)
Focus indicator (P.43)
Still image format (P.31)
Scale (P.45)
Sound of movie (P.42)
Scale orientation (P.45)
Scale position (P.45)
Resolution tab
Display tab