Optika Italy B-150D Series User manual

Model
B-150D-MRPL
B-150D-BRPL
B-150D Series
INSTRUCTION MANUAL
Ver. 2.3 2022

Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-150D-MRPL 4
3.2 B-150D-BRPL 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
7.1 B-150D-MRPL 6
7.2 B-150D-BRPL 7
8. Assembling 8
8.1 Assembling procedure 8
8.2 Polarizing set (optional) 8
9. Use of the microscope 10
9.1 Light intensity adjustment 10
9.2 Coarse focus tension adjustment 10
9.3 Stage 10
9.4 Adjust the interpupillary distance 10
9.5 Diopter adjustment 11
9.6 Use of oil immersion objective 11
9.7 Aperture diaphragm 12
9.8 Use with rechargeable batteries 12
9.9 Use of the polarizer (optional) 12
10. Use of software and digital head 13
11. Maintenance 14
12. Troubleshooting 15
Equipment disposal 16
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-150D-MRPL
3.2 B-150D-BRPL
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X)
⑥ Dustcover
⑦ Greenlter
⑧ Frostedlter
⑨ Powersupply
⑩ USBcable
③
④
⑤
⑥
⑦⑧
①
②⑨
⑩
③
④
⑤
⑥⑦⑧
①
②
① Frame
② Binocularobservationhead
③ Eyepieces
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X/100X)
⑥ Dustcover
⑦ Greenlter
⑧ Frostedlter
⑨ Powersupply
⑩ USBcable
⑪Immersionoil
⑨
⑩
⑪

Page 5
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 6
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH
/ INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
7. Instrument description
7.1 B-150D-MRPL

Page 7
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECES
OBSERVATION
HEAD
MAIN SWITCH
/ INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
7.2 B-150D-BRPL

Page 8
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 1ig. 1
①
FFig. 4ig. 4
①
8. Assembling
8.1 Assembling procedure
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw
①. (Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert one end of the USB cable into the connector on the
back of the optical head and the other end into one of the
USB ports on your computer.
4. Insert both eyepieces into the tubes of the optical head. (Fig.
2)
• For model B-150D-MRPL: only one eyepiece is used as
this model is monocular.
5. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 3)
8.2 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 4)

Page 9
FFig. 6ig. 6
③
FFig. 5ig. 5
②
2. Loosen the head xing knob ② and remove the head from
the microscope frame. (Fig. 5)
3. Insert the analyser into the hole inside the frame ③. (Fig. 6)
4. Put back the head into its original position and lock the xing
knob.

Page 10
FFig. 7ig. 7
①
FFig. 8ig. 8
②
FFig. 10ig. 10
④
FFig. 9ig. 9
③
9. Use of the microscope
9.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to turn ON / OFF
the microscope and to increase / decrease the illumination volt-
age. (Fig. 7)
9.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring ② using the provided tool (Fig. 8).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
9.3 Stage
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17 mm. (Fig. 9)
1. Open the spring arm of the slide holder ③ and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
9.4 Adjust the interpupillary distance
Only B-150D-BRPL
Hold the right and left parts of the observation head using both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen. (Fig. 10)
• The graduation on the interpupillary distance indicator ④,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 48- 75 mm.

Page 11
FFig. 11ig. 11
①
FFig. 12ig. 12
9.5 Diopter adjustment
Only B-150D-BRPL
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the dioptric adjustment ring ①to compen-
sate. (Fig. 11)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s dioptric correction.
9.6 Use of oil immersion objective
Only B-150D-BRPL
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 12)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the specimen would
be di󰀩cult (even not impossible) due to the presence of
an additional thickness on the objective.

Page 12
9.7 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Move the diaphragm lever ① (Fig. 13) to-
ward left or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 14. FFig. 13ig. 13
①
FFig. 14ig. 14
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 15ig. 15
②
9.8 Use with rechargeable batteries
When the microscope is plugged with the power supply, the LED
indicator for the battery recharge ② is lit. (Fig. 15)
• Power supply connected, low battery: led turns RED. When
charging is finished, it turns GREEN.
• Power supply not connected, microscope off: LED is always
OFF.
• Power supply not connected, microscope on, battery
charged: LED is GREEN.
• Power supply not connected, microscope on, battery almost
empty: LED is RED.
9.9 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.

Page 13
10. Use of software and digital head
The camera inside the digital head is driven by PROVIEW software.
For the instructions about the use of the software, please refer to the specic instruction manual.
Manual can be downloaded using the QR code available on this manual or using the web site.
The PDF version of the manual can be found under the name:
OPTIKA - B-150D - B-190TB - B-290TB Software Setup - Instruction manual - EN IT ES FR DE PT.

Page 14
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o󰀨-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or near
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
11. Maintenance

Page 15
12. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries are uncharged Fully charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position
Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
Batteries are uncharged Fully charge the batteries
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation.
Upon looking into eyepieces, try look-
ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.

Page 16
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sep-
arately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to pre-
vent possible adverse e󰀨ects on the environment and health and promotes reuse and/or recycling of materials of the equip-
ment. Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.
Equipment disposal

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
®
®
®
®

Modelli
B-150D-MRPL
B-150D-BRPL
Serie B-150D
MANUALE DI ISTRUZIONI
Ver. 2.3 2022

Pagina 19
1. Avvertenza 20
2. Informazioni sulla sicurezza 20
3. Contenuto della confezione 21
3.1 B-150D-MRPL 21
3.2 B-150D-BRPL 21
4. Disimballaggio 22
5. Utilizzo previsto 22
6. Simboli 22
7. Descrizione dello strumento 23
7.1 B-150D-MRPL 23
7.2 B-150D-BRPL 24
8. Assemblaggio 25
8.1 Procedura di assemblaggio 25
8.2 Set di polarizzazione (opzionale) 25
9. Uso del microscopio 27
9.1 Regolazione intensità luminosa 27
9.2 Regolazione della frizione 27
9.3 Tavolino 27
9.4 Regolazione distanza interpupillare 27
9.5 Regolazione diottrica 28
9.6 Uso di obiettivi ad immersione 28
9.7 Diaframma di apertura 29
9.8 Uso con batterie ricaricabili 29
9.9 Uso con polarizzatore (opzionale) 29
10. Uso del software e della testa digitale 30
11. Manutenzione 31
12. Risoluzione dei problemi 32
Smaltimento 33
Sommario

Pagina 20
1. Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato quotidianamente.
Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la manutenzione dello strumento,
e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
2. Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale coincida con il
voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “OFF”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli utilizzatori sono
gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello strumento è importante attenersi
alle seguenti istruzioni e leggere il manuale in tutte le sue parti.