Optika Italy B-290 Series User manual

Model
B-290 series (B-292/B-292PLI/B-293/B-293PLI)
B-290LD series (B-292LD1.50/B-292LD1/B-293LD1.50/B-293LD1)
B-290TB series (B-290TB)
B-290 Series
INSTRUCTION MANUAL
Ver. 5.5 2022

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Instrument description 4
5.1 B-292/B-292PLI/B-293/B-293PL 4
5.2 B-292LD1.50/B-292LD1/B-293LD1.50/B-293LD1 5
5.3 B-290TB 6
6. Unpacking 7
6.1 B-292/B-292PLI/B-293/B-293PLI 7
6.2 B-292LD1/B-292LD1.50/B-293LD1/B-293LD1.50 8
6.3 B-290TB 9
7. Assembling 10
7.1 Assembling the microscope 10
7.1.1 B-292/B-292PLI/B-293/B-293PLI 10
7.1.2 B-292LD1/B-292LD1.50/B-293LD1/B-2932LD1.50 11
7.1.3 B-290TB 12
7.2 Polarizing set (optional) 14
7.3 Phase contrast set (optional) 15
8. Use of the microscope 16
8.1 Switching on the microscope 16
8.2 Light intensity adjustment 16
8.3 Coarse focus tension adjustment 16
8.4 Stage 16
8.5 Adjust the interpupillary distance 17
8.6 Diopter adjustment 17
8.7 Use of oil immersion objective 17
8.8 Condenser centering 18
8.9 Aperture diaphragm 18
8.10 Useofuorescence 19
8.11 Use of the polarizer (optional) 19
8.12 Phase contrast observation (optional) 20
8.12.1 BrighteldObservation(BF) 20
8.12.2 Phase Contrast Observation (PH) 20
9. Microphotography 22
9.1 Cameras with projection lens 22
9.2 Reexcamera 22
10. Use of software and digital head 23
11. Micrometric Slide M-005 23
12. Maintenance 24
13. Troubleshooting 25
Equipment disposal 26
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.

Page 4
FINE FOCUS KNOB
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
INTENSITY ADJUSTMENT DIAL
STAGE
OBJECTIVES
COARSE FOCUS KNOB
CONDENSER
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
OBSERVATION HEAD
-) BINOCULAR (B-292/B-292PLI)
-) TRINOCULAR (B-293/B-293PLI)
EYEPIECES
APERTURE DIAPHRAGM
5. Instrument description
5.1 B-292/B-292PLI/B-293/B-293PL

Page 5
INTENSITY ADJUSTMENT DIAL
COARSE FOCUS KNOB
FINE FOCUS KNOB
CONDENSER
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
STAGE
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
EYEPIECES
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
OBSERVATION HEAD
-) BINOCULAR (B-292LD1/B-292LD1.50)
-) TRINOCULAR (B-293LD1/B-293LD1.50)
FLUORESCENCE
ATTACHMENT
APERTURE DIAPHRAGM
5.2 B-292LD1.50/B-292LD1/B-293LD1.50/B-293LD1

Page 6
INTENSITY ADJUSTMENT DIAL
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER OBJECTIVES
DIGITAL HEAD
TABLET
EYEPIECES
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
APERTURE DIAPHRAGM
5.3 B-290TB
HEADPHONE
MICRO HDMI
POWER
USB-A3.0
MICRO SD
CARD SLOT
USB TYPE C

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get damaged.
Using both hands (one around the arm and one around the base), lift the microscope from the container and put it on a
stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other residuals
may deteriorate the nal image quality and corrode the optics surface in a short time.
Once opened the box, the microscope parts are the following:
6.1 B-292/B-292PLI/B-293/B-293PLI
① Frame
② Observationhead
• binocular(B-292/B-292PLI)
• trinocular(B-293/B-293PLI)
③ Phototube(onlyB-293series)
④ Eyepieces
⑤ Objectives(4X/10X/40X/100X)
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
⑨ Tensionadjustmenttool
③
④
⑤
⑥
⑦
⑧
①
②
⑨

Page 8
① Frame
② Observationhead
• binocular(B-292LD1/B-292LD1.50)
• trinocular(B-293/B-293PLI)
③ Phototube(onlyB-293series)
④ Eyepieces
⑤ Objectives
• 10X/20X/40X/50X:B-292LD1.50/B-293LD1.50
• 10X/20X/40X/100X(dry):B-292LD1/B-293LD1
⑥ Dustcover
⑦ Fluorescenceattachment
⑧ Powersupply
⑨ Tensionadjustmenttool
③
④
⑤
⑥
⑦
⑧
①
②
⑨
6.2 B-292LD1/B-292LD1.50/B-293LD1/B-293LD1.50

Page 9
6.3 B-290TB
① Frame
② Digitalobservationhead
③ Eyepieces
④ Objectives(4X/10X/40X/100X)
⑤ Dustcover
⑥ Immersionoil
⑦ Powersupply
⑧ Tensionadjustmenttool
⑨ TabletPCpowersupply
⑩ USBcable0,5m
⑪ TabletPC
NOTE: OPTIKA reserves the right to make corrections, modications, enhancements, improvements and other
changes to its products at any time without notice.
③
④
⑤
⑥
⑦
⑧
①
⑩
⑪
②
⑨

Page 10
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4
7. Assembling
7.1 Assembling the microscope
7.1.1 B-292/B-292PLI/B-293/B-293PLI
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw.
(Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical head. (Fig.
2)
4. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 3)
Only for trinocular head
5. Unscrew the protection cap mounted on the photo port and
screw the photo tube. (Fig. 4)

Page 11
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 8ig. 8
FFig. 7ig. 7
7.1.2 B-292LD1/B-292LD1.50/B-293LD1/B-2932LD1.50
1. Insert the uorescence attachment above the frame and
tighten the locking screw. (Fig. 5)
2. Insert the cable in the socket placed at the rear side of the
microscope. (Fig. 6)
3. Insert the optical head above the stand and tighten the screw.
(Fig. 7)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
4. Insert both eyepieces into the tubes of the optical head. (Fig.
8)

Page 12
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
FFig. 12ig. 12
5. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 9)
Only for trinocular head
6. Unscrew the protection cap mounted on the photo port and
screw the photo tube. (Fig. 10)
7.1.3 B-290TB
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw.
(Fig. 11)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical head. (Fig.
12)
4. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 9)

Page 13
FFig. 13ig. 13
FFig. 14ig. 14
①
5. Fix the rotating part of the junction using the black wing-nut
①. (Fig. 13)
FFig. 15ig. 15
②
FFig. 16ig. 16
③
④
6. Then hook the Tablet PC onto the 4 screws of the junction
and pull toward down to rmly lock the Tablet PC in the hold-
er. (Fig. 14)
• To unlock the Tablet PC proceed with the opposite operation:
push toward up and remove it from the holder.
7. Plug one side of the USB cable ②to the digital head and
the other side to the Tablet PC using the connector ③. (Fig.
15-16).
8. Plug the power supply cable to the Tablet PC for battery re-
charge using the connector ④. (Fig. 16)
• The Tablet has been set with the Rotation function disabled:
this prevents any ipping of the Live View in order to get a
continuous and as large as possible view of your slide also
when the Tablet is removed from the holder.
• To enable this function again: you can activate the Rotation
by swiping the screen on his bottom right side and selecting
Settings + Screen. Anyway, it’s not suggested to activate the
function when the camera is in Live View mode as it may give
troubles when the camera runs at high resolutions.

Page 14
FFig. 19ig. 19
③
FFig. 17ig. 17
①
FFig. 18ig. 18
②
7.2 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 17)
2. Loosen the head xing knob ② and remove the head from
the microscope frame. (Fig. 18)
3. Insert the analyzer into the hole inside the frame ③. (Fig. 19)
4. Put back the head into its original position and lock the xing
knob.

Page 15
7.3 Phase contrast set (optional)
1. Remove the brighteld condenser by loosing the locking
screw ① on the right side of the condenser holder. (Fig. 20)
2. Insert the round dovetail of the phase contrast condenser
into the empty slot of the condenser holder and lock the lock-
ing screw ①.
FFig. 21ig. 21
FFig. 20ig. 20
①
3. Screw the phase contrast objectives in the revolving nose-
piece. (Fig. 21)

Page 16
8. Use of the microscope
FFig. 22ig. 22
FFig. 23ig. 23
FFig. 24ig. 24
FFig. 25ig. 25
②
①
8.1 Switching on the microscope
1. Operate on the main switch ①placed in the rear side of the
microscope, moving the selector on “I” (Fig. 22)
• For “LD” models only: there is a three-position switch on the
rear of the stand: position “I” turns on the transmitted light,
position “II” turns on the uorescence and position “O” turns
o󰀨 the microscope.
8.2 Light intensity adjustment
1. Operate on the light intensity dial to increase or decrease the
illumination intensity. (Fig. 23)
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is preset in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring using the provided tool (Fig. 24).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
8.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17mm. (Fig. 25)
1. Open the spring arm of the slide holder ② and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.

Page 17
8.5 Adjust the interpupillary distance
Hold the right and left parts of the observation head using both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen. (Fig. 26)
• The graduation on the interpupillary distance indicator ①,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 48- 75 mm.
FFig. 26ig. 26
FFig. 28ig. 28
①
FFig. 27ig. 27
②
8.6 Diopter adjustment
This operation can be done only on binocular models.
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the diopter adjustment ring ②to compen-
sate. (Fig. 27)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s diopter correction.
8.7 Use of oil immersion objective
AllmodelsexceptLDseries
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 28)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper towel or a
slightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the specimen would
bedi󰀩cult(evennotimpossible)duetothepresenceof
an additional thickness on the objective.

Page 18
8.8 Condenser centering
• The condenser is installed and pre-centered in the factory.
• To remove the condenser use an Allen wrench 1.5 mm and
operate on the xing knob placed on the right side of the
condenser holder.
Should a new centering is needed, operate in this way:
1. Insert 4x objective in the light path (in case 4x is not available
use the lower magnication available).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ①, moving the
ring to the value “4” related to the 4x objective. (Fig. 29)
4. Raise the condenser to the upper limit using the height ad-
justment knob ② placed on the left side of the condenser
holder.
5. Center the condenser using the centering screws ③ until the
eld of view is evenly illuminated (in the eld of view no dark
and bright areas must be noticed).
6. Fully open the diaphragm.
FFig. 30ig. 30
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 29ig. 29
①
②
③
8.9 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Move the diaphragm ring ①(Fig. 29) on
the value corresponding to the objective in use. In this case
the optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 30.

Page 19
8.10 Useofuorescence
1. Operate on the main switch placed in the rear side of the
microscope.
• Setting on “I” turns on transmitted light, setting on “II” turns
on uorescence. Setting on “O” turns o󰀨 the microscope.
(Fig. 31)
FFig. 31ig. 31
FFig. 32ig. 32
2. Move the lter selector in the “B” position (Fig. 32) to insert
the uorescence lter in the light path. Move the selector in
the middle to work with brighteld transmitted light.
• Unlike a mercury lamp system, B-290LD LED illumination
doesn’t need any power-up time for heating, and can be used
immediately after switching on. Also, the LED source is pre-
aligned in factory and doesn’t need any alignment operation.
3. Focus on your sample, and adjust the light intensity as need-
ed through the brightness adjustment knob. In order to im-
prove the darkness of the background (thus improving con-
trast), it is strongly suggested to put a dark cover on the light
exit at the base of the microscope.
FILTER
NAME EXCITATION FILTER
DICHROIC MIR-
ROR EMISSION FILTER APPLICATIONS
B 460/490 nm 505 nm 515LP nm
• FITC: uorescent antibodies
• Achridine orange DNA/RNA
• Auramine
8.11 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.

Page 20
8.12 Phase contrast observation (optional)
Slider phase contrast condenser (Fig. 33) allows observation in
brighteld and in phase contrast with 10X/20X/40X objectives.
FFig. 34ig. 34
FFig. 35ig. 35
FFig. 33ig. 33
UPPER PART OF
CENTERING
TELESCOPE
Observation mode Slider position
Brighteld O
Phase contrast 10X/20X 10
Phase contrast 40X 40
8.12.1 BrighteldObservation(BF)
1. Move the condenser slider in the central position to insert the
empty hole.
2. Center the condenser as described in chapter 8.8 and start
working normally.
8.12.2 Phase Contrast Observation (PH)
1. Center the condenser as already described in the chapter
8.8.
2. Move the condenser slider all the way toward left to insert the
phase ring dedicated to 10X/20X objective. (Fig. 34)
3. Insert 10X or 20X objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.