Optika Italy B-190 Series User manual

Model
B-190PL Series (B-191PL / B-191SPL / B-192PL / B-192SPL / B-193PL)
B-190TBPL
B-190 Series
INSTRUCTION MANUAL
Ver. 7.4 2021

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Instrument description 4
5.1 B-191PL/B-191SPL/B-192PL/B-192SPL/B-193PL 4
5.2 B-190TBPL 5
6. Unpacking 6
6.1 B-191SPL/B-191PL/B-192SPL/B-192PL/B-193PL 6
6.2 B-190TBPL 7
7. Assembling 8
7.1 Assembling the microscope 8
7.1.1 B-191SPL/B-191PL/B-192SPL/B-192PL/B-193PL 8
7.1.2 B-190TBPL 9
7.2 Polarizing set (optional) 11
8. Use of the microscope 12
8.1 Switching on the microscope 12
8.2 Light intensity adjustment 12
8.3 Coarse focus tension adjustment 12
8.4 Stage 12
8.5 Adjust the interpupillary distance 13
8.6 Diopter adjustment 13
8.7 Use of oil immersion objective 13
8.8 Aperture diaphragm 14
8.9 Use of the polarizer (optional) 14
9. Microphotography 15
9.1 Cameras with projection lens 15
9.2 Reexcamera 15
10. Use of software and digital head 16
11. Micrometric Slide M-005 16
12. Maintenance 17
13. Troubleshooting 18
Equipment disposal 19
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.

Page 4
FINE FOCUS
KNOB
PHOTO TUBE
(ONLY B-193PL)
INTENSITY ADJUSTMENT
DIAL
STAGE
OBJECTIVES
COARSE FOCUS
KNOB
CONDENSER X/Y MOVEMENT
KNOBS
SLIDE HOLDER
APERTURE DIAPHRAGM
OBSERVATION HEAD
-) MONOCULAR (B-191PL / B-191SPL)
-) BINOCULAR (B-192PL / B-192SPL)
-) TRINOCULAR (B-193PL)
EYEPIECES
TENSION ADJUSTMENT
RING
5. Instrument description
5.1 B-191PL/B-191SPL/B-192PL/B-192SPL/B-193PL

Page 5
INTENSITY
ADJUSTMENT
DIAL
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
OBJECTIVES
DIGITAL HEAD
TABLET PC
EYEPIECES
TENSION ADJUSTMENT
RING
APERTURE DIAPHRAGM
5.2 B-190TBPL
HEADPHONE
MICRO HDMI
POWER
USB-A3.0
MICRO SD
CARD SLOT
USB TYPE C

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get damaged.
Using both hands (one around the arm and one around the base), lift the microscope from the container and put it on a
stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other residuals
may deteriorate the nal image quality and corrode the optics surface in a short time.
Once opened the box, the microscope parts are the following:
6.1 B-191SPL/B-191PL/B-192SPL/B-192PL/B-193PL
① Frame
② Observationhead
• monocular(B-191PL/B-191SPL)
• binocular(B-192PL/B-192SPL)
• trinocular(B-193PL)
③ Phototube(onlyB-193PL)
④ Eyepieces
⑤ Objectives
• 4X/10X/40X/100X(B-191PL/B-192PL/B-193PL)
• 4X/10X/40X/60X(B-191SPL/B-192SPL)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
⑨ Immersionoil(B-191PL/B-192PL/B-193PL)
⑩ Tensionadjustmenttool
③
④
⑤
⑥
⑦
⑧
①
②
⑨⑩

Page 7
① Frame
② Digitalobservationhead
③ Eyepieces
④ Objectives(4X/10X/40X/100X)
⑤ Dustcover
⑥ Greenlter
⑦ Immersionoil
⑧ Powersupply
⑨ Tensionadjustmenttool
⑩ TabletPCpowersupply
⑪ USBcable0,5m
⑫TouchpenforTabletPC
⑬ TabletPC
③
④
⑤
⑥
⑦
①
⑨
⑩
⑪
⑫
⑬
②
⑧
6.2 B-190TBPL

Page 8
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4
7. Assembling
7.1 Assembling the microscope
7.1.1 B-191SPL/B-191PL/B-192SPL/B-192PL/B-193PL
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw.
(Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical head. (Fig.
2)
4. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 3)
Only for B-193PL
5. Unscrew the protection cap mounted on the photo port and
screw the photo tube. (Fig. 4)

Page 9
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8
①
7.1.2 B-190TBPL
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the
screw. (Fig. 5)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical head. (Fig.
6)
4. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 3)
5. Fix the rotating part of the junction using the black wing-nut
①. (Fig. 7)
6. Then hook the Tablet PC onto the 4 screws of the junction
and pull toward down to rmly lock the Tablet PC in the hold-
er. (Fig. 8)
• To unlock the Tablet PC proceed with the opposite operation:
push toward up and remove it from the holder.

Page 10
FFig. 9ig. 9
②
7. Plug one side of the USB cable ②to the digital head and
the other side to the Tablet PC using the connector ③. (Fig.
9-10).
8. Plug the power supply cable to the Tablet PC for battery re-
charge using the connector ④. (Fig. 10)
• The Tablet has been set with the Rotation function disabled:
this prevents any ipping of the Live View in order to get a
continuous and as large as possible view of your slide also
when the Tablet is removed from the holder.
• To enable this function again: you can activate the Rotation
by swiping the screen on his bottom right side and selecting
Settings + Screen. Anyway, it’s not suggested to activate the
function when the camera is in Live View mode as it may give
troubles when the camera runs at high resolutions.
FFig. 10ig. 10
③
④

Page 11
FFig. 13ig. 13
③
FFig. 11ig. 11
①
FFig. 12ig. 12
②
7.2 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 11)
2. Loosen the head xing knob ② and remove the head from
the microscope frame. (Fig. 12)
3. Insert the analyzer into the hole inside the frame ③. (Fig. 13)
4. Put back the head into its original position and lock the xing
knob.

Page 12
FFig. 14ig. 14
FFig. 15ig. 15
FFig. 16ig. 16
FFig. 17ig. 17
②
①
8. Use of the microscope
8.1 Switching on the microscope
Operate on the main switch ①placed in the rear side of the mi-
croscope, moving the selector on “I” (Fig. 14)
8.2 Light intensity adjustment
Operate on the light intensity dial to increase or decrease the
illumination intensity. (Fig. 15)
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is preset in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring using the provided tool. (Fig. 16)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
8.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17 mm. (Fig. 17)
1. Open the spring arm of the slide holder ② and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.

Page 13
FFig. 18ig. 18
FFig. 20ig. 20
①
FFig. 19ig. 19
②
8.5 Adjust the interpupillary distance
• ExceptB-191PL/B-191SPL
Hold the right and left parts of the observation head using both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen. (Fig. 18)
• The graduation on the interpupillary distance indicator ①,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 48- 75 mm.
8.6 Diopter adjustment
• ExceptB-191PL/B-191SPL
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the diopter adjustment ring ②to compen-
sate. (Fig. 19)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s diopter correction.
8.7 Use of oil immersion objective
• B-191PL / B-192PL / B-193PL / B-190TBPL
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 20)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the specimen would
bedi󰀩cult(evennotimpossible)duetothepresenceof
an additional thickness on the objective.

Page 14
FFig. 22ig. 22
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 21ig. 21
①
8.8 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Move the diaphragm ring ①(Fig. 21) on
the value corresponding to the objective in use. In this case
the optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 22.
8.9 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.

Page 15
FFig. 23ig. 23
FFig. 24ig. 24
M1
M1) Screw the Reex adapter to the “T2” ring (provided with the
Reex camera or from its manufacturer)
FFig. 25ig. 25
M2
FFig. 27ig. 27
9. Microphotography
9.1 Cameras with projection lens
1. Remove dust caps from camera and projection lens.
2. Screw the projection lens to camera thread. (Fig. 23)
3. Insert the projection lens into the photo tube. (Fig. 24)
9.2 Reexcamera
1. Screw the “T2” ring (not provided) at the end of the projection
lens (M-173), then install the whole onto the reex camera.
(Fig. 25)
2. Insert the projection lens into the photo tube. (Fig. 27)

Page 16
10. Use of software and digital head
The camera inside the digital head is driven by PROVIEW software.
For the instructions about the use of the software, please refer to the specic instruction manual.
Manual can be downloaded using the QR code available on this manual or using the web site.
The PDF version of the manual can be found under the name:
OPTIKA - B-150D - B-190TB - B-290TB Software Setup - Instruction manual - EN IT ES FR DE PT.
11. Micrometric Slide M-005
For biological microscopes calibration
1 DIV=0.01mm
Micrometricslide,26x76mm,with2scales
(1mm/100div. for biological microscopes / 10mm/100div. for stereomicroscopes)
For stereo microscopes calibration
1 DIV=0.1mm
0 1 2 3 4 5 6 7 8 9 10

Page 17
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°-40°C and
a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o󰀨- position.
•
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note:ethanolandetherarehighlyammableliquids.Donotusethemnearaheatsource,nearsparksornear
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
12. Maintenance

Page 18
13. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position
Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
For transmitted light observation, the
coverglass thickness must not exceed
0.17mm
Use a coverglass with thickness
0.17mm
Focus is not even Slide holder is not flat. Move the spec-
imen to a flat position
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section:
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube:
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation.
Upon looking into eyepieces, try look-
ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.
V. Microphotography:
Image edge is unfocused To a certain extent it is due to achro-
matic objectives features To minimize the problem, set the aper- ture diaphragm in a proper position
Bright spots appear on the image Stray light entering in the microscope
through eyepieces or camera viewfind-
er.
Cover eyepieces and viewfinder with a
dark cloth

Page 19
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sep-
arately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to pre-
vent possible adverse e󰀨ects on the environment and health and promotes reuse and/or recycling of materials of the equip-
ment. Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
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