Optika IM-3MET User manual

Version: 3
Issued: 08, 08, 2017
Model
IM-3MET
IM Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-625 Eyepiece EWF10x/22mm.
M-601 Eyepiece WF15x/16mm.
M-602 Eyepiece micrometer EWF10x/22mm.
M-005 26x76 mm micrometric slide. Range 1 mm, div. 0,01 mm.
M-735 Objective IOS LWD MET PLAN 5x/0,15.
M-736 Objective IOS LWD MET PLAN 10x/0,3.
M-737 Objective IOS LWD MET PLAN 20x/0,45.
M-738 Objective IOS LWD MET PLAN 50x/0,55.
M-746 Objective IOS LWD MET PLAN 100x/0.80.
M-699 Universal adapter for M-114, M-116, M-173 and eyepiece cameras.
M-789 Focusable C-Mount adapter for 1/3” sensor.
M-789.1 Focusable C-Mount adapter for 1/2” sensor.
M-789.2 Focusable C-Mount adapter for 2/3” sensor.
M-622 Halogen bulb 12V/50W (only for IM-3M).
DC-004 TNT dust cover, large, 700(l)x550(h) mm.
M-792 Mechanical stage for IM-3 series.
M-793.1 Holder for Petri diameter 38mm (M-793.2 needed).
M-793.2 Holder for Terasaki and Petri diameter 65mm.
M-793.3 Holder for slide and Petri diameter 54mm.
M-793.4 Holder for 2+2 slides.
M-793.5 Holder for metallurgical samples.
M-793.7 Load-bearing side extension for IM-3 series.
M-114 C-Mount adapter for 1/2” sensor.
M-116 C-Mount adapter for 2/3” sensor.
M-173 Photo adapter for APS-C and Full Frame Reflex cameras.
M-788 Photo adapter for REFLEX camera with FULL FRAME sensor.
VP-IM IQ/OQ/PQ Validation Protocols.

Page 5
Overview
TRINOCULAR
VIEWING
TUBE
(FIXED)
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
LIGHT PATH
SELECTOR
LEVEL
COARSE
FOCUS KNOB
LIGHT
ADJUSTEMENT
FINE FOCUS KNOB
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
LAMP HOUSE
MICROSCOPE
BODY
METAL STAGE
INSERT

Page 6
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Microscope body
② Eyepieces
③ Objectives
④ Metal insert for stage
⑤ Lamp house
⑥ Brighteld lters (LBD and IF550)
⑦ Analyzer lter
⑧ Polarizer lter
⑨ Filter holder
⑩ Power cable
⑪ Power cable
⑪
⑩
③
④
⑤
⑥
⑦
⑧
⑨
①
②

Page 7
③
Fig.2
Fig.3
Fig.4
Fig.5
Fig.1
1. Turning the coarse focusing knob ① till the
nosepiece reaches its lowest position.
► For a safe transport, the nosepiece is
placed in the lowest position and the
tension adjustment collar ②is adjust-
ed to the appropriate tension when the
microscope leaves the factory. (Fig.1)
2. Screw the lowest magnication objective
on to the turret from the right side, then
turn the turret clockwise. Mount the other
objectives in the same way, following the
sequence from low to high.
► Note: the objectives can also be in-
stalled through the stage opening.
(Fig.2)
► Clean the objectives regularly. In inverted
microscopes, the objectives are very sen-
sitive to dust.
► To prevent dust and contamination from
entering the microscope, cover all the un-
used holes with dust caps ③. (Fig.3)
► When operating, use the low magnication
objective (10X) to search and focus the
specimen, then switch to higher magnica-
tions.
► When switching between objectives, slowly
turn the nosepiece until it clicks. The click
means that the objective is in the right posi-
tion, in the center of the light path.
①
②
Installing the objectives
The stage extension can be installed on either
side of the stage to enlarge the working sur-
face. The mechanical stage must be installed
on the side opposite the extension.
For right-handed operators, the mechanical
stage is normally installed on the right side.
1. Installing the stage extension: Screw the
bolts on to the extension, then mount the
extension from below the stage. (Fig.4)
2. Installing the mechanical stage: As for the
extension, the mechanical stage is xed
with two bolts under the stage. (Fig.5)
Installing the stage extension and
the mechanical stage (OPTIONAL)

Page 8
1. When using the metal stage, make sure
that the insert is horizontal.
2. Install the stage insert in the stage open-
ing. (Fig.6)
Installing the stage insert
Installing the eyepieces
Installing the lamp house
Insert both eyepieces into the tubes of the
optical head. (Fig.7)
Insert the lamp house and tighten the provi-
ded allen screws (Fig. 8)
Slide the lter holder into the tube in front of
the lamp. (Fig. 9).
Open the lamp door. (Fig. 10)
Fig.6
Fig.7
Fig.10
Fig.8
Fig.9

Page 9
1. Turn the main switch ① to “O”(off) before
connecting the power cord. (Fig.13)
2. Insert the cable into the power socket of
the microscope. (Fig.14)
3. Plug the power cord into the mains sock-
et. Check for a safe connection.
► Please use the supplied power cord.
If lost or damaged, please refer to
qualied service.
► Connect the power cord to a grounded
(earthed) power supply only.
Before replacing the fuse, turn the main switch
to “O” (off) and unplug the power cord.
Rotate the fuse support out of the holder using
a straight screwdriver. Insert a new fuse in the
support, then rotate the support back into the
holder. (Fig.15)
► Fuse rating:
see back of the microscope.
Connecting the power cord
Replacing the fuse
Insert the halogen lamp into its holder. Do
NOT touch the lamp bulb with the bare hands,
this could reduce the lamp efciency and
lifetime. (Fig. 11)
Plug the power cable to the lamp house.
(Fig. 12)
Fig.12
Fig.13
Fig.14
Fig.15
①
Fig.11

Page 10
Turning on the LED
Connect the power, turn on the main switch ①.
(Fig.16)
Adjusting the brightness
Turn the brightness adjustment knob ②to in-
crease and decrease the brightness. (Fig.17)
Adjusting the tension
► The coarse focusing knob (1) is pre
adjusted to a tight tension upon leav-
ing the factory.
If the nosepiece drops down by itself, or the
specimen defocuses while adjusting the ne
focus knob ③, the coarse focus knob is too
loose. Turning the tension adjustment collar ②
in clockwise direction tightens the coarse fo-
cus tension ①. Rotate in the opposite direction
to decrease the tension.
Setting the specimen
► For the best image quality, use asks,
Petri dishes and slides with a 1.2 mm
thickness.
1. Place the proper insert for your specimen
(according to the table on the right) on the
stage, and x it with the stage clip.
2. Turning the X and Y knobs, move the
specimen to the required position. (Move-
ment Range: 120 (width) × 78 (length)
mm).
Moving the specimen
Move the specimen to the desired position by
freehand or by turning the knobs of the me-
chanical stage.
► When switching objectives, take care
not to touch the adaptor plates with
the objectives, as their weight may
damage the front lens.
M-793.1
Holder for Petri diameter 38mm (M-793.2 needed)
M-793.2
Holder for Terasaki and Petri diameter 65mm.
M-793.3
Holder for slide and Petri diameter 54mm.
M-793.4
Holder for 2+2 slides.
M-793.5
Holder for metallurgical samples.
M-793.6
Holder for Utermöhl-Chamber (M-793.3 needed).
M-792
Mechanical stage for IM-3 series.
INITIEL SETUP
STAGE (OPTIONAL)
Using the microscope
Fig.16
Fig.17
①
②
①
②
③
Fig.18
M-793.8
Load-bearing side extension for IM-3 series.

Page 11
Dioptric adjustment
1. Look into the right eyepiece with your right
eye only, and focus on the specimen.
2. Look into the left eyepiece with your left
eye only. If the image is not sharp, use
the dioptric adjustment ring ①to compen-
sate. (Fig.19)
► The adjustment range is ±5 diopter.
The number indicated on the adjust-
ment ring graduation should corre-
spond to the operator’s dioptric cor-
rection.
Adjusting the interpupillary distance
Observing with both eyes, hold the two eye-
piece prism assemblies. Rotate them around
their common axis until the elds of view co-
incide.
► The graduation on the interpupillary
distance indicator ②, pointed by the
spot “.” on the eyepiece holder, shows
the distance between the operator’s
eyes. (Fig.20)
The range of the interpupillary distance is 48-
75mm.
Selecting the light path
Pull the light path selector lever ③ sideways
using your thumb, selecting the light path you
need. (Fig.21)
VIEWING TUBE
②
Fig.19
Fig.20
Fig.21
LIGHT PATH SELECTOR
LEVER
BRIGHTNESS APPLICATION
In 20% used for binocular observation, and
80% used for video or photography
Binocular observation, television, and
micrography or video can be operated
simultaneously
Out 100% used for binocular
observation
Binocular
observation
③
①

Page 12
Using color lters
Selecting the appropriate color lters accord-
ing your need. (Fig.22)
If you need to work with polarized incident
light, insert the polarizer ler into the slot in
front of the lamp (Fig. 23)
Insert the analyzer lter into the slot under the
objectives (Fig. 24).
Using the aperture and eld diaphragms
When in brighteld observation, the aperture
diaphragm controls the numerical aperture of
the illumination system. When the numerical
aperture of the objective and the aperture of
the illumination system match, the highest res-
olution is achieved.
The aperture can be changed by moving the
aperture adjustment lever ① (Fig.25)
Generally, when observing a fully chromatic
specimen, you need to set the size of the con-
denser to 70-80% of the aperture of the objec-
tive.
Fully close the eld diaphragm ②.
(Fig.25) (Fig.26 A).
Act on the diaphragm centering screws ③ un-
til you move the bright spot in the center of the
image eld (Fig.25) (Fig.26 B).
Then re-open the eld diaphragm ②.
(Fig.25) (Fig.26 C)
ILLUMINATION UNIT
Fig.22
Fig.23
Fig.24
Fig.25
①
Ⓐ Ⓑ Ⓒ
②
③
CENTERING THE CONDENSER
Fig.26

Page 13
Installing the photography adapter
1. To activate the video port, pull the light
path selector lever to “In” position. (Fig.27)
2. Loosen the locking bolt ①on the trinocu-
lar viewing tube, and take out the dust cap
②. (Fig.28)
3. Install the photography adapter into the
trinocular port according to its instruc-
tions, and screw down the locking bolt ①.
(Fig.29)
4. Attach the camera ring (if any) to the
adapter.
5. Attach the camera to the ring.
● Warning: for some cameras (mainly re-
ex) the ring is not included with the mi-
croscope, and it should be supplied by the
user.
● For the photography of dark specimens,
obscure the eyepieces and the viewnder
with a dark cloth in order to reduce stray
light.
● The camera magnication can be calcu-
lated as objective magnication × camera
+ lens magnication.
► When shooting with a SLR, the mirror
movement may cause camera move-
ment. Please lift the mirror, use long
exposure times and use an extension
cord.
MICROPHOTOGRAPHY
Fig.27
Fig.28
Fig.29
①
①
Video
Camera
CCD
Camera head
Adapter
Adapter
②
②

Page 14
Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
• Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 15
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
The illumination is open, but the eld
of view is dark.
The plug of the LED holder is
not connected to the illumina-
tion set
Connect them
The brightness is too low Adjust to a proper setting
Too many colour lters have
been stacked
Minimize the number of the lters
The edge of the eld of view is
vignetted or the brightness is asym-
metric.
The nosepiece is not in the cor-
rect position
Turn the nosepiece to a click stop
The color lter is partially
inserted
Insert the lter to full depth
Dust and stains can be seen in the
eld of view.
There are dust or dirt on the
specimen
Clean the specimen
There are dust or dirt on the
specimen
Clean the eyepiece
There is an apparent double image. The size of the aperture dia-
phragm is too small
Open the aperture diaphragm
Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The phase contrast is low.
The nosepiece is not in the
center of the light path
Turn the nosepiece to a click stop
The aperture diaphragm in
the view of eld is opened too
much or too little
Adjust the aperture diaphragm
The lenses (objective, eyepiec-
es) are dirty
Thoroughly clean all the optical system
The phase contrast depends on
the sample position
The sample holder is not at. Move the
sample around until a compatible area is
found.

Page 16
One side of the image is out of focus. The nosepiece is not in the
center of the light path
Turn the nosepiece to a click stop
The specimen is out of place
(tilted)
Place the specimen at on the stage.
The optical performance of the
sample cover glass is poor
Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to
turn.
The tension adjustment collar is
too tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is
too loose
Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is
low
Adjust the brightness
The light blinks The power cord is poorly con-
nected
Check the power cord
IV. Viewing tube assembly
The eld of view of the two eyes is
different
The interpupillar distance is not
correct
Adjust the interpupillar distance
The dioptric correction is not
right
Adjust the dioptric correction
The viewing technique is not
correct, and the operator is
straining the eyesight
When look into the objective, do not stare
at the specimen but look at the whole eld
of view. Periodically, move the eyes away
to look at a distant object, then back into
the objective
V. Microphotography and video
The image is unfocused Incorrect focussing Adjusting the focus system as in the pres-
ent manual
The edge of the image is unfo-
cussed
To some degree, it is inherent
to the nature of achromatic
objectives
The problem can be minimized by a correct
setting of the aperture diaphragm
Bright patches appear on the im-
age
Stray light is entering the
microscope through the eye-
pieces and through the camera
viewnder
Cover the eyepieces and the viewnder
with a dark cloth

Page 17
Equipment disposal
Art.13 Dlsg 25 july 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste.
The separate collection of this equipment at the end of its lifetime is organized and managed by the producer.
The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection.
The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to prevent
possible adverse effects on the environment and health and promotes reuse and/or recycling of materials of the
equipment.
Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.

Page 18

Page 19

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