Optika B-810 Series User manual

Model
B-810
B-810 Series
INSTRUCTION MANUAL
v 1.0 2018

Page 2
Table of Contents
1. Warning
2. Symbols and conventions
3. Safety Information
4. Overview
5. Unpacking
6. Assembling
7. Summary of brighteld observation procedures (B-810BF)
8. Using the microscope
9. Use of universal condenser for brighteld / darkeld / phase contrast
10.Use of slide condenser for light field / phase contrast
11. Microphotography
12. Maintenance
13. Troubleshooting
Equipment disposal

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.

Page 4
4. Overview
COARSE
FOCUSING KNOB
NOSEPIECE
DIOPTRIC ADJUSTMENT
RING
PHOTO PORT
MAIN ON/OFF
EYEPIECE
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
BRIGHTNESS ADJUSTMENT
KNOB
FOCUS-STOP KNOB
FINE FOCUSING KNOB
OBJECTIVE
STAGE
FOCUS LOCK
LEVER
X/Y MOVEMENT
KNOBS

Page 5
5. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
6. Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Power supply
⑥ Stage
⑦ Objectives
①②
③
④
⑤
⑥
⑦

Page 6
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 3)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Put the microscope stand on a
solid table. Insert the optical
head above the attachment,
using the 2mm Allen wrench
to tighten the screw. (Fig.1)
Insert both eyepieces into
the tubes of the optical head.
(Fig.2)
Fig.1
Fig.2
Fig.3
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.4)
Fig.4

Page 7
Insert the power supply jack
on the connector at the rear.
(Fig.5)
Fig.5

Page 8
7. Summary of brighteld observation procedures (B-510BF)
① Main switch /
brightness adjustment knob
(Used commands) (Chapter)
②Slide holder
③ X/Y movement knobs
④Nosepiece
⑦Binocular tube
⑧Diopter adjustment ring
⑨Condenser height adjustment
knob
⑩ Condenser centering screws
⑪Aperture diaphragm
⑫Field diaphragm
④Nosepiece
① Main switch /
brightness adjustment knob
Corse ⑤and fine ⑥
focus knobs
Corse ⑤and fine ⑥
focus knobs
Set the main swith to “ON” and adjust light intensity.
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/9)
(9)
(5)
(5)
(5)
(5)
(5)
(9)
(9)
(9)
(9)
(9)
(9)
(5/9)

Page 9
8. Using the microscope
In order to activate the transmitted light illu-
minator, insert the plug of the external power
supply into the socket and turn on the main
switch on the side of the main body. Turn the
brightness adjustment knob to a brightness
suitable for observation. (Fig.6)
Loosen the lock-screw, turn the observation
head to a comfortable position for observation,
and then lock the lock-screw. (Fig.7)
LED settings
Adjust the observation
head
MAIN SWITCH
Fig.6
Fig.7
Fig.8
①
1. Look into the right eyepiece with your right
eye only, and focus on the specimen.
2. Look into the left eyepiece with your
left eye only. If the image is not sharp,
use the dioptric adjustment ring ①to
compensate. (Fig.8)
► The adjustment range is ±5 diopter. The
number indicated on the adjustment
ring graduation should correspond to
the operator’s dioptric correction.
Dioptric adjustment

Page 10
Stage
Stage accepts standard slides 26 x 76 mm,
thickness 1,2 mm with coverlside 0,17mm.
It is possible to place two slides side by side
on the stage.
• Open the spring arm of the slide
holder and place frontally the slides
on the stage.
• Gently release the spring arm of the
slide holder. (Fig. 12)
► A sudden release of the the spring
arm could cause the falling of the
slide.
Fig.12
Observing with both eyes, hold the two
eyepiece prism assemblies. Rotate them
around their common axis until the elds of
view coincide. (Fig.9)
Adjusting the interpupillary
distance
Fig.9
Fig.10
Fig.11
• Use with eyeglasses (Fig. 10)
Fold rubber eyeshields with both hands.
Folded eyeshields avoid scratching the
lenses of eyeglasses.
• Use without eyeglasses (Fig. 11)
Raise eye shields and observe at the
microscope placing eyes to the shields,
avoiding external light to disturb the
observation.
Use of eye shields

Page 11
Centering the condenser (Fig.13)
1. Place the specimen on the stage, insert
10x objective into the light path and
focus.
2. Insert the front lens of the swing-out
condenser ①.
3. Rotate the eld diaphragm ring ②in the
direction showed by the arrow, to fully
close the diaphragm.
4. Rotate the condenser height adjustment
knob ③ to focus the edges of the
diaphragm.
5. Rotate the two centering screws ④to
bring the bright spot in the center of the
eld of view.
6. Gradually open the diaphragm. The
condenser is centered when the
diaphragm image is symmetrical to the
eld of view.
7. In normal use, open the diaphragm until
it circoscribes the eld of view.
Effects of the eld diaphragm
Field diaphragm adjusts the illuminated area
to obtain a high contrast image.
Set the diaphragm according to the objective
in use until it circoscribes the eld of view,
in order to eliminate unnecessary light to
eyepieces.
Aperture diaphragm (Fig. 13-14)
• The Numerical Aperture (N.A.) value of
the aperture diaphragm affects the image
contrast. Increasing or reducing this value
one can vary resolution, contrast and
depth of focus of the image.
• With low contrast specimens set the
numerical aperture value ① (printed on
the condenser ring) to about 70%-80% of
the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty
sleeve, adjust the condenser’s ring in
order to obtain an image like the one in
g. 15.
Example: with objective PLAN 40x /
0,65 set the scale to 0.65 x 0.8 = 0,52
CENTERING THE CONDENSER
①
③
④
②
Fig.13
Fig.14
Fig.15
①
IMAGE OF
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Uso del condensatore

Page 12
1. Focus the specimen with a low power
objective.
2. Lower the stage (remembering to lock the
coarse upper limit knob).
► Put a drop of oil (provided) on the area
of the specimen to be observed. Make
sure that there are no oil bubbles. Air
bubbles in the oil damage the image
quality.
• To check for bubbles: remove an eyepiece,
fully open the aperture diaphragm and
observe the objective exit pupil. (The pupil
must be round and bright).
• To remove the bubbles, gently move the
nosepiece to the right and left to move the
immersion objective a few times and allow
the air bubbles to move.
3. Insert immersion objective.
4. Return the stage to the upper focusing
point and obtain an optimal focus using
the ne focus knob.
5. After use, gently remove the oil with a soft
paper towel or a lightly moistened optic
paper with a mixture of ethyl ether (70%)
and absolute ethyl alcohol (30%).
► The immersion oil, if not immediately
cleaned, could crystallize creating a
glass-like layer.
In this situation the observation of
the specimen would be difcult if not
impossible due to the presence of an
additional thickness on the objective.
Fig.16
Use of oil immersion objective
(Fig. 16)

Page 13
Focus tension
adjustment
Turn the tension-adjust knob
to get a suitable tension for
the focus system. (Fig.17)
NOTE: if the tension is too lo-
ose, the stage could go lower
by itself or the focus easily
lost after ne adjustment. In
this case, rotate the knob in
order to increase the friction.
Fig.17
Focus-stop knob
Loosen the focus-stop knob,
rotate the coarse focusing
knob to bring the slide into
focus with a 4X or 10X objecti-
ve. Then rotate the focus-stop
in order to block the height of
the stage. This simplies the
next focusing operations.
The focus-stop knob is also
useful to avoid accidental con-
tacts between objective and
specimen. (Fig.18) Fig.18

Page 14
1) BRIGHTFIELD OBSERVATION (BF)
Rotate the condenser turret to insert the “BF”position. Now repeat the steps described in the procedure “Summary of
brighteld observation procedures” at pag.47.
2) DARKFIELD OBSERVATION (DF)
1. Rotate the condenser turret to insert the “DF”position.
2. Open the aperture diaphragm.
3. Place a specimen on the stage and focus.
4. Observing into eyepieces raise or lower the condenser until a homogeneous illumination of the specimen can be
achieved, thus obtaining a proper darkeld effect.
• Darkeld requires a huge amount of light. Switching from darkeld to brighteld, one could be dazzled. Do not
keep your eyes on the eyepieces when moving the condenser turret from DF to BF.
• “Dry” darkeld observation, that is, without the use of oil, is only possible with objectives with N.A. lower than
0,7.
• Observing in darkeld, it may be necessary to raise the condenser from the normal position to obtain a more
homogeneous illumination. This is not a defect.
9. Use of universal condenser for brighteld/darkeld/phase contrast
Universal condenser provided with B-810PH allows observation in brighteld, darkeld and phase
contrast.
OBSERVATION MODE Condenser Turret position
Brighteld BF (Fig. 17)
Darkeld DF (Fig. 18)
Phase contrast (10x) 10/20 (Fig.19)
Phase contrast (20x) 10/20 (Fig.19)
Phase contrast (40x) 40 (Fig. 20)
Phase contrast (100x) 100 (Fig. 21)
Fig.19 Fig.20
Fig.23Fig.22Fig.21

Page 15
3) PHASE CONTRAST OBSERVATION
(PH)
1. Center the condenser as already
described at pag. 50.
2. Rotate the condeser turret to insert the
“10/20” position.
3. Insert 10x objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.
6. Remove one eyepiece and insert the
centering telescope. (Fig.24)
7. Rotate the upper part of the centering
telescope until the two phase rings
(one dark and one bright) visible in the
telescope are in focus. (Fig. 25)
8. Using centering screws on the condenser
①, center the phase rings to make the
bright ring ② be concentric to the dark
ring ③.
9. Insert 20x objective (do not rotate the
condenser turret) and check the centering
of the two rings. (Fig. 26)
10. Repeate the same operation with other
objectives to check the ring centering:
40x objective – turret position “40”, 100x
objective – turret position “100”.
11. At the end remove the centering
telescope, reinstall the eyepiece and
begin observation.
• With 40x and 100x objectives it may be
useful to slightly raise the condenser,
to obtain a better projection of the
phase rings. This is not a defect.
• With the 4X objective, the condenser
could have a dark halo at the periphery
of the eld of view. This is not to be
considered a defect.
Use of the green lter (Fig. 28)
• The green lter is used to increase the
contrast of the image during phase
contrast observation.
• Place the lter on the eld lens of the
microscope (Fig. 27) and begin the
observation.
• For observation in brighteld or darkeld
it is advisable to remove the lter from the
optical path.
Fig.25
Fig.24
UPPER PART OF CENTERING
TELESCOPE
CONDENSER PHASE
RING (BRIGHT) ②
OBJECTIVE PHASE
RING (DARK) ③
Fig.26
Fig.27
Fig.28
①

Page 16
1) BRIGHTFIELD OBSERVATION (BF)
Rotate the condenser turret to insert the “BF”position. Now repeat the steps described in the procedure “Summary of
brighteld observation procedures”.
10. Use of slide condenser for light eld / phase contrast
Universal condenser provided with B-810PH allows observation in brighteld, darkeld and phase
contrast.
OBSERVATION MODE Condenser Turret position
Brighteld BF (Fig. 29)
Phase contrast (10x) 10 (Fig.30)
Phase contrast (40x) 40 (Fig.31)
Fig.29
Fig.31
Fig.30

Page 17
2) OBSERVATION IN PHASE CONTRAST
(PH)
1. Center the condenser as described on
page 25.
2. Move the condenser slide to insert the
“10” position
3. Insert the 10x objective into the optical
path.
4. Open the aperture diaphragm.
5. Place a sample on the table and focus.
6. Remove an eyepiece and insert the
centering telescope (Fig.32)
7. Rotate the top of the telescope to focus on
the rings (one light and one dark) visible in
the telescope. (Fig. 33)
8. Using the centering screws on the slide ①
center the rings so that the clear ring ② is
concentric to the dark ring ③.
9. Repeat the operation with the 40x
objective: 40x objective - slide position
“40”.
10. At the end remove the centering telescope,
reposition the eyepiece and start the
observation.
• With the 40x objective, it may be useful
to slightly raise the condenser, to
obtain a better projection of the phase
rings. This is not a defect.
Fig.33
Fig.32
UPPER PART OF CENTERING
TELESCOPE
CONDENSER PHASE
RING (BRIGHT) ②
OBJECTIVE PHASE
RING (DARK) ③
Fig.34

Page 18
Installing the C-mount adapter
1. Loosen the clamping screw ①on the
trinocular port and remove the dust cap
②. (Fig.36)
2. Screw the C-mount adapter ③to the
camera ④ and insert the round dovetail
of the C-mount into the empty hole of the
trinocular port, then tighten the clamping
screw ①. (Fig.37)
Use of Reex camera
1. Insert the Reex adapter ① into the relay
tube to the microscope ②.
2. Screw the “T2” ring ③ (not provided) to
the reex adapter.
3. Connect the Reex camera ④ to the “T2”
just installed (Fig. 38).
● “T2” ring is not privided along with the
microscope, but is commercialy available.
● While shhoting dark specimens, darken
eyepieces and viewnder with a dark cloth
to miminize the diffused light.
● To calculate the magnication of the
camera: objective magnication * camera
magnication * lens magnication.
► If using an SLR camera, mirror
movement may cause the camera to
vibrate.
We suggest lifting the mirror, using
long exposure times and a remote
cord.
11. Microphotography
Fig.27
Fig.37
①
②
①
②
Fig.36
①
②
Fig.38
①
②
③
④
③
④

Page 19
12. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
• Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 20
13. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
1. Optical System
LED does not light. Power cord is unplugged. Plug power cord into the power outlet.
LED operates, but eld of view
remains dark.
Aperture and eld iris diaphragms are
not opened wide enough.
Adjust them to proper sizes.
Condenser is lowered too much. Adjust the condenser height position.
Light path selector knob is set to the
camera position.
Move the knob to the eye position.
Field of view is obscured or
not evenly illuminated
Light path selector knob is in an inter-
mediate position.
Set the knob according to the observation
method.
Revolving nosepiece is not correctly
engaged.
Make sure that the revolving nosepiece
clicks properly into place.
Condenser is not attached properly. Re-attach it.
An objective that falls outside of the
condenser’s illumination range is used.
Use a condenser to match the purpose.
Condenser is not properly centered. Center the condenser.
Field iris diaphragm is stopped down
too far.
Open the field iris diaphragm until it circum-
scribes the field.
Dirt or dust is visible in the
field of view.
Dirt/dust on the eyepieces Clean thoroughly.
Dirt or the to surface of the condenser
Dirt/dust on the specimen
Visibility is poor.
· Image is not poor.
· Contrast is poor.
· Details are indistinct.
· Image glares.
Condenser is lowered too far. Adjust the condenser height position.
Aperture iris diaphragm is stopped
down too far.
Open aperture iris diaphragm.
Front lens of objective is dirty. Clean objective.
Immersion oil is not being used with an
oil immersion objective.
Use immersion oil.
Immersion oil contains bubbles. Remove the bubbles.
Recommended immersion oil is not
used.
Use the provided immersion oil.
Dirt/dust on specimen. Clean it.
Dirt/dust on condenser
Inappropriate object side or cover
glass thickness.
Replace with glass of recommended thick-
ness.