Oxford Instruments ANDOR MicroPoint 4 User manual
8.2 x 8.2 mm / 512 x 512 sensor
Lowest Noise Imaging EMCCD
MicroPoint 4
© Andor Technology Ltd. 2023
Version 3.1 rev 25 September 2023
andor.oxinst.com
User Guide
For model: MP-420
version 3.1 rev 25 SEPTEMBER 2023
2
SAFETY AND WARNING INFORMATION.......................................................................................6
Laser Safety Special Warnings .......................................................................................................... 8
Section 1: Introduction...........................................................................................................................13
1.1 MicroPoint 4..........................................................................................................................................................................13
1.2 Help and Technical Support.....................................................................................................................................14
1.3 Disclaimer.............................................................................................................................................................................15
1.4 Copyright and Protective Notices.........................................................................................................................15
1.5 Trademarks and Patent Information ..................................................................................................................15
1.6 Supplied Components MicroPoint 4....................................................................................................................16
1.7 Accessories ..........................................................................................................................................................................17
1.7.1 Core Unit, Laser Sources and Adapters..................................................................................................................... 17
1.7.2 Laser Dyes......................................................................................................................................................................................... 18
1.7.3 MicroPoint Laser and Microscope Interlock Components........................................................................... 19
1.7.4 CW Laser Sources for MicroPoint 4 Fibre Spot Adapter................................................................................ 21
............................................................ 22
23
........................................................................................................................... 25
................................................. 26
................................................ 27
Section 2: Product Overview ............................................................................................................. 29
2.1 Hardware Description................................................................................................................................................29
2.1.1 Intended Use ................................................................................................................................................................................... 30
2.1.2 NL100 Laser Rear Panel........................................................................................................................................................ 30
2.2 Electrical Connections, Optical Connections and Interlocks.............................................................31
2.2.1 Electrical Connections ............................................................................................................................................................. 31
....................................................... 32
.......................................................................................................................................... 33
..................................................................................................................................... 34
............................................................................................................................ 35
................................................................................................................................................................... 36
2.2.3 Interlock Connections............................................................................................................................................................... 37
................................................................................... 38
2.2.5 Back Panel on MicroPoint 4 Controller....................................................................................................................... 39
2.2.6 Front Panel on MicroPoint 4 Controller....................................................................................................................... 40
version 3.1 rev 25 SEPTEMBER 2023
3
2.3 Lasers..............................................................................................................................................................................40
2.3.1 Laser Intensity Control................................................................................................................................................................40
....................................................................................................................................................................40
Section 3: Installation...............................................................................................................................41
3.1 Location ..................................................................................................................................................................................41
............................................................................................................................................................................................41
3.2 Assembly...............................................................................................................................................................................41
3.3 PC Workstation Operating System Notes........................................................................................................41
3.4 Software.................................................................................................................................................................................41
Section 4: Operation ............................................................................................................................... 42
4.1 Overview................................................................................................................................................................................ 42
4.2 Emergency Mains Disconnection......................................................................................................................... 42
4.3 Power-up Sequence ..................................................................................................................................................... 42
4.4 Power-down Sequence .............................................................................................................................................. 42
4.5 The Dye Cell........................................................................................................................................................................ 43
4.6 To Remove and Install the Dye Cell.................................................................................................................... 44
4.7 Dye Cell Lifetime.............................................................................................................................................................. 44
4.8 Replacing the Laser Dye............................................................................................................................................ 45
4.9 Replacement of Dye with Dierent Type of Dye ........................................................................................ 45
4.10 Selecting a Lamp House Port and Beamsplitter Plug......................................................................... 46
4.11 Inserting the Magnetic Beam Splitter.............................................................................................................. 46
4.12 Removing the Magnetic Beam Splitter ......................................................................................................... 46
4.13 Inserting Excitation Filters....................................................................................................................................... 47
4.14 Laser Centration with Target................................................................................................................................. 48
4.15 Focusing the MicroPoint............................................................................................................................................51
4.16 Laser Intensity Control .............................................................................................................................................. 52
4.17 Pulse Frequency and Number.............................................................................................................................. 53
4.18 Using the MicroPoint 4 Piezo Oset Feature................................................................................... 54
4.19 Interlocks and Errors .................................................................................................................................................. 54
4.20 Risk Mitigation .............................................................................................................................................................. 55
4.21 The MicroPoint 4 LCD Controller ........................................................................................................................ 55
4.22 Using MicroPoint 4 with Andor iQ ...................................................................................................................... 55
4.23 Operation of iQ Imaging Software.................................................................................................................... 56
version 3.1 rev 25 SEPTEMBER 2023
4
Section 5: Maintenance and User Service Procedures..................................................... 58
5.1 Cleaning and Decontamination............................................................................................................................. 58
.....................................................................................................................................58
5.2 System Alignment........................................................................................................................................................... 59
5.3 Regular Checks ................................................................................................................................................................ 59
On a Daily Basis:......................................................................................................................................... 59
On a Weekly Basis: ................................................................................................................................... 59
5.3.1 Minimum Computer Requirements..................................................................................................................................59
........................................................................................................................................59
5.4 Maintenance Packages..............................................................................................................................................60
5.5 Service Options.................................................................................................................................................................60
................................................................................................................60
Section 6: Troubleshooting...................................................................................................................61
6.1 Troubleshooting Examples ........................................................................................................................................61
Appendix A: Technical Data MicroPoint 4.................................................................................. 65
Appendix B: Mechanical Drawings................................................................................................69
Appendix C: Other Information ......................................................................................................... 72
Appendix D: MicroPoint 4 China RoHS Hazardous Substances Declaration..... 73
version 3.1 rev 25 SEPTEMBER 2023
5
Version Released Description
3.0 19/9/23 Initial release of MicroPoint 4
3.1 25/9/23
Manuals for Components
Nitro NL100 Laser
CW laser
CW laser
version 3.1 rev 25 SEPTEMBER 2023
6
SAFETY AND WARNING INFORMATION
PLEASE READ THIS INFORMATION FIRST
BEFORE USING YOUR PRODUCT.
CAUTION - USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES
OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION
EXPOSURE.
1.
may be impaired.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
of patients.
18.
version 3.1 rev 25 SEPTEMBER 2023
7
19.
20.
21.
care.
22.
23.
24.
25.
26.
27.
customer support.
28.
29.
30.
version 3.1 rev 25 SEPTEMBER 2023
8
Laser Safety Special Warnings
READ AND DO NOT IGNORE!
CAUTION – USE OF CONTROLS OR ADJUSTMENTS OR
PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN
MAY RESULT IN HAZARDOUS RADIATION EXPOSURE
•
•
laser source used.
•
MicroPoint, See section 5.
•
• WARNING:Class 3B
be
•
•
•
•
product.
•
•
version 3.1 rev 25 SEPTEMBER 2023
9
•
•
•
completely safe.
•
•
•
•
• “prevents
human access to [hazardous] laser radiation (including errant laser radiation)… except when human access
is necessary for the performance of the function(s) of the product.”
•
•
IEC TR 60285-14 Safety
of laser products – Part 14: A User’s Guide and the American National Standard for the Safe Use of Lasers
(ANSI Z136.1) are standard references for best practice.
•
IEC 60285-1 Safety of
laser products – Part 1: Equipment Classication and Requirements.
•
measures.
•
•
expected.
•
version 3.1 rev 25 SEPTEMBER 2023
10
Laser Product Safety Standards
•
reasonably practicable.
•
Laser Safety Protection Measures
Emission LEDs
5
5
•
•
Safety Interlocks
•
•
•
•
•
version 3.1 rev 25 SEPTEMBER 2023
11
Laser Aperture Locations
•
•
•
•
•
Access Panels
•
•
•
•
•
version 3.1 rev 25 SEPTEMBER 2023
12
•
•
•
•
•
•
•
•
laser radiation.
Label Symbols
EU WEEE
version 3.1 rev 25 SEPTEMBER 2023
13
Section 1: Introduction
1.1 MicroPoint 4
Figure 1: MicroPoint 4
MicroPoint Nitro
MicroPoint Pico
galvanometer models only.
• manual
• galvanometer
rd
version 3.1 rev 25 SEPTEMBER 2023
14
Europe USA
Andor Technology Ltd.
7 Millennium Way
Belfast
BT12 7AL
Andor Technology
Suite # 150
Concord
MA 01742
USA
Asia-Pacic China
Andor Technology (Japan)
Japan
Andor Technology (China)
Haidian District,
100089
Contact and Support
1.2 Help and Technical Support
version 3.1 rev 25 SEPTEMBER 2023
15
1.3 Disclaimer
HEREIN.
1.4 Copyright and Protective Notices
1.5 Trademarks and Patent Information
Manufacturers Information
Andor Technology Ltd., Belfast, BT12 7AL, UK.
version 3.1 rev 25 SEPTEMBER 2023
16
1.6 Supplied Components MicroPoint 4
Table 1: Standard components supplied with MicroPoint 4.
Quantity Quantity
1
1
1
1
1 1
1 1
Micropoint 4 Controller 1 NL100 Interlock Cable. 1
Remote Controller
1
1
2HLE Interlock Cable 1
2
1
1 1
User Guide
1
version 3.1 rev 25 SEPTEMBER 2023
17
1.7 Accessories
information.
1.7.1 Core Unit, Laser Sources and Adapters
Table 2: Core Units Part Numbers
MicroPoint 4 Galvo
Select Core System based on your Microscope
Zeiss Axio, Leica DM,
All Nikon models
Leica DMI8,
Evident/Olympus IX/BX
Zeiss Axiovert 200 Evident/Olympus:
BX51/WI model
MicroPoint 4 Galvo with
Nitro Dye Laser
MP-4203-NGO MP-4204-NGO MP-4205-NGO MP-4206-NGO
COMING SOON
MicroPoint 4 Galvo with
Pico Laser
MP-4203-PGO MP-4204-PGO MP-4205-PGO MP-4206-PGO
FSA
MP-4210-FSA-S
MicroPoint 4 Manual Select Core System based on your Microscope
Zeiss Axio, Leica DM,
All Nikon models
Leica DMI8,
Evident/Olympus IX/BX
Zeiss Axiovert 200 Evident/Olympus:
BX51/WI model
MicroPoint 4 Manual with
Nitro Dye Laser
MP-4203-NMA MP-4204-NMA MP-4205-NMA MP-4206-NMA
FSA
MP-4210-FSA-S
Step 1. Choose your control interface, laser and microscope.
Select one of these or none if FSA only
Core Unit
MP-4203-NGO to MP-4206-NGO
MP-4203-PGO to MP-4206-PGO
MP-4203-NMA to MP-4206-NMA
Step 2. Add optional CW laser if required to any MP-420X model
FSA and CW lasers
MP-4210-FSA-S
See to order CW lasers
Step 3. Select one pulsed laser or none if FSA only
Pulsed Laser
MP-N2CE-PSIA
MP-NDYAG-1NS
version 3.1 rev 25 SEPTEMBER 2023
18
1.7.2 Laser Dyes
Table 3: Laser Dye Part Numbers
MicroPoint Laser Dyes
Dye Part Number Centre (±1) Wavelength/nm Eciency (Relative to 435nm)/%
MP-27-365-DYE 365 66
MP-27-388-DYE 388 46
MP-27-390-DYE 390 60
MP-27-404-DYE 404 38
MP-27-419-DYE 419 40
MP-27-422-DYE 422 60
MP-27-435-DYE 435 100
MP-27-471-DYE 471 70
MP-27-481-DYE 481 64
MP-27-514-DYE 514 50
MP-27-521-DYE 521 76
MP-27-539-DYE 539 70
MP-27-543-DYE 543 30
MP-27-551-DYE 551 84
MP-27-576-DYE 576 54
MP-27-582-DYE 582 69
MP-27-590-DYE 590 73
MP-27-593-DYE 593 52
MP-27-613-DYE 613 42
MP-27-622-DYE 622 56
MP-27-626-DYE 626 71
MP-27-651-DYE 651 52
MP-27-656-DYE 656 52
version 3.1 rev 25 SEPTEMBER 2023
19
1.7.3 MicroPoint Laser and Microscope Interlock Components
products.
Note: when more than one laser source is used in an instrument, an interlock combiner is required.
Table 4: Interlock Components. Andor will choose which of these you need from information you supply.
Interlock Components
Product Codes Description
IL-BX53
IL-BX53-ROD-2POS
IL-BX53-ROD-UTTR
IL-COMB
Interlock Combiner Kit
IL-COMB-CL4
IL-DF
IL-DM6
Leica DM6 Interlock Adaptor Kit
IL-DMI8
Leica DMi8 Interlock Kit
IL-DMI8-COMBO
IL-DSK
IL-EX1M
Modular Interlock 1 m Extension
IL-EX30
Modular Interlock 30 cm Extension
IL-EX60
Modular Interlock 60 cm Extension
IL-ILE
ILE Primary Interlock Cable Kit
IL-ILE-2
ILE Primary/Secondary Interlock Cable
IL-IMUX
IL-INV
IL-IX71
IL-IX81
IL-IX83
IL-LDMI
Leica DMi3/4/5/6000 Interlock Kit
IL-LLM5-FLOCK
IL-LLOCK
IL-LMM5-FLOCK
version 3.1 rev 25 SEPTEMBER 2023
20
IL-MEBS
IL-MIDU
IL-MINF
IL-MMAR
IL-MMBD
Mosaic Body Interlock Replacement Kit
IL-MMDU
Mosaic Duet Body Interlock Replacement
IL-MMLD
Mosaic Laser Diode Interlock Replacement
IL-MOS-DUET-UPG
IL-MOS-UPG
IL-MPNC
IL-MPNL
IL-NC2+
Nikon C2+ Interlock Adaptor
IL-NCA1
IL-NFN1
IL-NNIU
IL-NTI2
Nikon Ti2 Interlock Kit
IL-NTI2
IL-NTIU
IL-PCUB
IL-RIC
IL-RVXD
ALC/PCU Interlock Cable
IL-TEST-KIT
IL-UPR
IL-WDB
IL-ZAXI
NOTE: MicroPoint laser interlock parts may require use of a combiner when used with other laser products
This manual suits for next models
1
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