Thermo Scientific IMH 60 User manual

Thermo Scientific
Heratherm
Advanced Protocol and
Advanced Protocol Security
Microbiological Incubators

IMH 60/100/180/400/750
IMH 60-S/100-S/180-S/400-S/750-S
Operating Instructions
50125555 D 05.08.2019
50125555 E 12.01.2021

ii Heratherm Thermo Scientific
©2021 Thermo Fisher Scientific Inc. Todos los derechos reservados.
Estas instrucciones de operación están protegidas por derechos de autor. Los derechos resultantes del mismo, en particu-
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El contenido de este manual de operación puede ser modificado en cualquier momento y sin aviso previo. En cuanto a
traducciones en lenguas extranjeras, la versión alemana de estas instrucciones de operación se considera vinculante.
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Heratherm®es una marca registrada de Thermo Scientific.
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Todas las demás marcas registradas que se mencionan en las instrucciones de operación son de propiedad exclusiva de los
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Thermo Scientific Heratherm i
C
Chapter 1 Safety Notes ..................................................................................................................... 1-1
Basic Operating Precautions ............................................................................................................... 1-1
Operational Safety Rules .................................................................................................................... 1-2
Warranty ............................................................................................................................................ 1-2
Explanation of Safety Information and Symbols................................................................................. 1-3
Safety Notes and Symbols used throughout these Operating Instructions....................................... 1-3
Additional symbols for safety information ...................................................................................... 1-4
Symbols on the Incubator............................................................................................................... 1-5
Intended Purpose of the Incubator ..................................................................................................... 1-6
Intended Purpose of the Incubator ................................................................................................. 1-6
Incorrect Use .................................................................................................................................. 1-6
Standards and Directives .................................................................................................................... 1-6
Chapter 2 Delivery of the Incubator................................................................................................. 2-1
Packaging ........................................................................................................................................... 2-1
Acceptance Inspection ........................................................................................................................ 2-1
Scope of Supply.................................................................................................................................. 2-2
Chapter 3 Installation........................................................................................................................ 3-1
Ambient Conditions........................................................................................................................... 3-1
Location Requirements................................................................................................................... 3-1
Intermediate Storage........................................................................................................................... 3-2
Room Ventilation............................................................................................................................... 3-2
Space Requirement......................................................................................................................... 3-4
Installation Built-in Units............................................................................................................... 3-4
Table-top units............................................................................................................................... 3-5
Floor stand units............................................................................................................................. 3-6
Transport ........................................................................................................................................... 3-8
Stacking Kit...................................................................................................................................... 3-10
Installing the Stacking Feet............................................................................................................... 3-10
Installing the Stacking Adapter......................................................................................................... 3-11
Installing the Anti-tilt Anchor .......................................................................................................... 3-12
Floor stand unit spacers .................................................................................................................... 3-14
Chapter 4 Product Description ........................................................................................................ 4-1
Heratherm IMH Series Incubator Overview....................................................................................... 4-1
Heratherm IMH-S Incubator Overview ............................................................................................. 4-4
Safety Devices................................................................................................................................... 4-11
Work Space Atmosphere .................................................................................................................. 4-11
Door switch...................................................................................................................................... 4-11
Sensing and Control System............................................................................................................. 4-12
Contents

Contents
ii Heratherm Thermo Scientific
Data Communications & Alarm Interface........................................................................................ 4-13
RS 232 interface ........................................................................................................................... 4-13
Alarm contact ............................................................................................................................... 4-13
Mains connection ......................................................................................................................... 4-13
Fuses............................................................................................................................................. 4-13
Work Space Components................................................................................................................. 4-14
Inner Chamber............................................................................................................................. 4-14
Connecting Nozzles for Fresh-air Filters (Accessory Items)........................................................... 4-14
Access port.................................................................................................................................... 4-15
Tube Access Ports............................................................................................................................. 4-17
Chapter 5 Installation procedures ................................................................................................... 5-1
Table-top units................................................................................................................................... 5-1
Installing the Shelf System.................................................................................................................. 5-1
Initial installation ........................................................................................................................... 5-1
Installing the Perforated Shelves ......................................................................................................... 5-2
Preparing the useable space................................................................................................................. 5-2
Installation or Removal of the Support Rails................................................................................... 5-3
Installing and Uninstalling the Air Baffle........................................................................................ 5-4
Levelling the Incubator................................................................................................................... 5-4
Commissioning of floor-mounted units.............................................................................................. 5-5
Installing/Removing air baffles (IMH-S Series)............................................................................... 5-5
Commissioning, general ..................................................................................................................... 5-6
Installing the Shelf Support Brackets .............................................................................................. 5-6
Installing the Perforated Shelves ..................................................................................................... 5-7
Mains connection............................................................................................................................... 5-8
Connection to the power supply source.......................................................................................... 5-8
Connecting the RS-232 Interface ....................................................................................................... 5-9
Interconnecting the Incubator with a Computer........................................................................... 5-10
Wiring the Alarm Contact................................................................................................................ 5-11
Function Description ................................................................................................................... 5-11
Alarm Relay Specifications............................................................................................................ 5-11
Connection Example .................................................................................................................... 5-12
Chapter 6 Operation.......................................................................................................................... 6-1
Preparing the Incubator...................................................................................................................... 6-1
Starting Operation.............................................................................................................................. 6-1
Chapter 7 Handling and Control ...................................................................................................... 7-1
Powering Up ...................................................................................................................................... 7-6
Switching the Incubator Off / Powering Down .................................................................................. 7-6
Temperature Set Value ....................................................................................................................... 7-7
Timer ................................................................................................................................................. 7-8
Setting a Fixed-time On or Off Timer.......................................................................................... 7-10
Setting a Weekly Timer................................................................................................................ 7-11
Stopping a Timer.......................................................................................................................... 7-13
Light................................................................................................................................................. 7-15
Decontamination.............................................................................................................................. 7-16
Fan ................................................................................................................................................... 7-19
Table-top units................................................................................................................................. 7-19

Contents
Thermo Scientific Heratherm iii
Floor stand units ...............................................................................................................................7-20
Settings .............................................................................................................................................7-22
Error Log ......................................................................................................................................7-22
Calibration ....................................................................................................................................7-23
Date and Time..............................................................................................................................7-24
Temperature Display Unit ............................................................................................................7-27
Chapter 8 Shut-down .........................................................................................................................8-1
Shutting the Incubator Down .............................................................................................................8-1
Chapter 9 Cleaning and Disinfection ...............................................................................................9-1
Cleaning..............................................................................................................................................9-1
Cleaning Exterior Surfaces...............................................................................................................9-1
Wipe / Spray Disinfection...................................................................................................................9-1
Preparing the manual wipe/spray disinfection..................................................................................9-2
Predisinfection ................................................................................................................................9-3
Cleaning..........................................................................................................................................9-4
Final disinfection.............................................................................................................................9-4
Starting the Decontamination Routine............................................................................................9-4
Chapter 10 Maintenance....................................................................................................................10-1
Inspections and checks ......................................................................................................................10-1
Regular Checks..............................................................................................................................10-1
Semi-annual Inspection.................................................................................................................10-1
Service Intervals.................................................................................................................................10-2
3-monthly service ..........................................................................................................................10-2
Annual service ...............................................................................................................................10-2
Preparing Temperature Calibration...................................................................................................10-3
Comparison Measurement Procedure................................................................................................10-3
Temperature Calibration Procedure ..................................................................................................10-4
Replacing the Door Seal....................................................................................................................10-4
Replacing the Power Cord.................................................................................................................10-5
Returns for Repair.............................................................................................................................10-5
Chapter 11 Disposal...........................................................................................................................11-1
Overview of Materials Used ..............................................................................................................11-1
Chapter 12 Error Codes.....................................................................................................................12-1
Chapter 13 Technical Data ................................................................................................................13-1
Chapter 14 Spare Parts and Accessories ........................................................................................14-1
Chapter 15 Device Log ......................................................................................................................15-1
Chapter 16 Contact ............................................................................................................................16-1

Contents
iv Heratherm Thermo Scientific

Thermo Scientific Heratherm i
L
Figure 3-1 Table-top incubators, dimensions and required clearances.................................................................... 3-5
Figure 3-2 Floor stand incubators, dimensions and required clearances.................................................................. 3-6
Figure 3-3 Floor stand incubators, dimensions and required clearances.................................................................. 3-7
Figure 3-4 Lift Points............................................................................................................................................. 3-8
Figure 4-1 Heratherm IMH 60/ IMH 100/ IMH 180 Front View........................................................................4-2
Figure 4-2 Heratherm IMH 60/ IMH 100/ IMH 180 Rear View.......................................................................... 4-3
Figure 4-3 Heratherm IMH 60-S/ IMH 100-S/ IMH 180-S Series Front View .................................................... 4-5
Figure 4-4 Heratherm IMH 60-S / IMH 100-S / IMH 180-S Series Rear View.................................................... 4-6
Figure 4-5 Heratherm IMH 400-S Series Front View............................................................................................ 4-7
Figure 4-6 Heratherm IMH 400-S Rear View ....................................................................................................... 4-8
Figure 4-7 Heratherm IMH 750-S Front View...................................................................................................... 4-9
Figure 4-8 Heratherm IMH 750-S Rear View ....................................................................................................... 4-10
Figure 4-9 Sensor System (for table-top units) ....................................................................................................... 4-12
Figure 4-10 Sensor System (for floor stand units) .................................................................................................. 4-12
Figure 4-11 Signal Interfaces and Power Socket..................................................................................................... 4-13
Figure 4-12 Connecting Nozzles for Fresh-air Filters (Accessory Items) ................................................................. 4-15
Figure 4-13 Shelf System ....................................................................................................................................... 4-16
Figure 4-14 Tube Access Ports............................................................................................................................... 4-17
Figure 5-1 Sliding the Retaining Spring into the Support Rail ............................................................................... 5-1
Figure 5-2 Installing the Shelving .......................................................................................................................... 5-2
Figure 5-3 Support Rail Installation....................................................................................................................... 5-3
Figure 5-4 Removing the Air Baffle ....................................................................................................................... 5-4
Figure 5-5 Removing the bottom plate .................................................................................................................. 5-5
Figure 5-6 Removing the left and right support profiles......................................................................................... 5-5
Figure 5-7 Removing the rear air baffle IMH 400-S / 750-S.................................................................................. 5-6
Figure 5-8 Shelf Support Bracket Installation ........................................................................................................ 5-7
Figure 5-9 Installing the Perforated Shelves ........................................................................................................... 5-7
Figure 5-10 AC Power Supply Socket ................................................................................................................... 5-9
Figure 5-11 Alarm Relay Connection Example ...................................................................................................... 5-12
Figure 7-1 Control Panel for Heratherm IMH Series and IMH-S Series Incubators .............................................. 7-1
Figure 10-1 Door Seal Replacement ...................................................................................................................... 10-4
List of Figures

List of Figures
ii Heratherm Thermo Scientific

Thermo Scientific Heratherm 1-1
1
Safety Notes
Basic Operating Precautions
These operating instructions describe Heratherm incubators.
Heratherm incubators have been manufactured to the latest state of the art and have been
tested thoroughly for flawless functioning prior to shipping. However, the incubator may
present potential hazards, particularly if it is operated by inadequately trained personnel or if it
is not used in accordance with the intended purpose. Therefore, the following must be
observed for the sake of accident prevention:
• Never step into the unit.
• Heratherm incubators must be operated by adequately trained and authorized professional
personnel.
• Heratherm incubators must not be operated unless these operating instructions have been
fully read and understood.
• The present operating instructions, applicable safety data sheets, plant hygiene guidelines
and the corresponding technical rules issued by the operator shall be used to create written
procedures targeted at personnel working with the subject matter device, detailing:
• the decontamination measures to be employed for the incubator and the accessories
used with it,
• the safety precautions to be taken when processing specific agents,
• the measures to be taken in case of accidents.
• Repair work on the incubator must be carried out only by trained and authorized expert
personnel.
• The contents of these operating instructions are subject to change at any time without
further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the incubator so that safety instructions and
important information are always accessible.
• Should you encounter problems that are not detailed adequately in these operating
instructions, please contact Thermo Electron LED GmbH immediately for your own safety.

Safety Notes
Operational Safety Rules
1-2 Heratherm Thermo Scientific
Operational Safety Rules
The following rules must be heeded when working with Heratherm incubators:
• Observe the sample weight limits specified for your Heratherm incubator as a whole and
its shelving in particular; see “Technical Data” on page 13-1.
• Do not load the bottom of the interior workspace to avoid the risk of overheating any
samples placed there and to prevent the temperature sensor from being damaged.
• Arrange the samples evenly throughout the work space, making sure not to place them too
closely to the interior walls to ensure a uniform temperature distribution.
• Do not load your Heratherm incubator with substances that exceed the capabilities of the
available lab apparatus and Personal Protection Equipment to provide sufficient degrees of
protection to users and third parties.
• Check the door seal every six months for proper sealing performance and possible damage.
• Do not process any samples containing hazardous chemical substances that may be
released into the ambient air through defective seals or may cause corrosion or other
defects on parts of the Heratherm incubator.
Warranty
Thermo Fisher Scientific warrants the operational safety and functions of the Heratherm
incubators only under the condition that:
• the incubator is operated and serviced exclusively in accordance with its intended purpose
and as described in these operating instructions,
• the incubator is not modified,
• only original spare parts and accessories that have been approved by Thermo Scientific are
used (third-party spares without Thermo Scientific approval void the limited warranty),
• inspections and maintenance are performed at the specified intervals,
• an operation verification test is performed after each repair activity.
The warranty is valid from the date of delivery of the incubator to the customer.

Safety Notes
Explanation of Safety Information and Symbols
Thermo Scientific Heratherm 1-3
Explanation of Safety Information and Symbols
Safety Notes and Symbols used throughout these Operating Instructions


Indicates a hazardous situation which, if not avoided, will result
in death or serious injuries.


Indicates a hazardous situation which, if not avoided, could result
in death or serious injuries.


Indicates a situation which, if not avoided, could result in damage to
equipment or property.


Is used for useful hints and information regarding the application.

Safety Notes
Explanation of Safety Information and Symbols
1-4 Heratherm Thermo Scientific
Additional symbols for safety information
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electrical shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!

Biological hazard!

Contamination hazard!

Danger of tipping!

Safety Notes
Explanation of Safety Information and Symbols
Thermo Scientific Heratherm 1-5
Symbols on the Incubator


Observe operating instructions



VDE test mark

CE-conformity mark: confirms conformity according to EU Guidelines

Alarm contact

Safety Notes
Intended Purpose of the Incubator
1-6 Heratherm Thermo Scientific
Intended Purpose of the Incubator
Intended Purpose of the Incubator
Heratherm incubators are laboratory devices for preparing and cultivating cell and tissue
cultures. The devices employ precision temperature control for simulating the specific
physiological ambient conditions for these cultures.
Incorrect Use
To avoid the risk of explosion do not load the incubator with tissue, material, or liquids that:
• are easily flammable or explosive,
• release vapor or dust that forms combustible or explosive mixtures when exposed to air,
• release poisons,
• release dust,
• exhibit exothermic reactions,
• are pyrotechnical substances.
• refrain also from pouring any liquids onto the internal base plate or inserting bowls filled
with liquids into the sample compartment.
Standards and Directives
The incubator complies with the following standards and guidelines:
• IEC EN 61010 - 1, IEC EN 61010 - 2 - 010
• Low Voltage Directive 2006/95/EC
• EMC Directive 2004/108/EC
Additionally, the incubator is in compliance with many other international standards,
regulations and directives not listed here. Should you have any questions regarding compliance
with national standards, regulations and directives applicable for your country, please contact
your Thermo Fisher Scientific sales organization.

Thermo Scientific Heratherm 2-1
2
Delivery of the Incubator
Packaging
Heratherm incubators are delivered in a rugged packaging box. All packaging materials can be
separated and are reusable:
Packaging materials
Packaging carton: Recycled paper
Foam elements: Styrofoam (CFC-free)
Pallet: Chemically untreated wood
Packaging film: Polyethylene
Packaging ribbons: Polypropylene
Acceptance Inspection
After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damage.
If components are missing or damage is found on the device or the packaging, in
particular damage caused by humidity and/or water, please notify the carrier as well as
Thermo Scientific Technical Support immediately.
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere
on the device, take all necessary precautions to protect
personnel handling the incubator. For example, have them wear
protective gloves and other personal protection equipment.

Delivery of the Incubator
Scope of Supply
2-2 Heratherm Thermo Scientific
Scope of Supply
Incubators
Quantity of components supplied (pieces) IMH Series
IMH-S Series
Perforated shelves 2
Support rail for shelf table-top units 2
Shelf support 4
Power cord 1
Connector, potential-free contact 1
Clip springs for table-top units 2
Plug 1
Anti-tilt anchor 1
Operating manual 1
Short reference guide 1

Thermo Scientific Heratherm 3-1
3
Installation
Ambient Conditions
Location Requirements
Built-in units of incubators can, heating and drying ovens must be operated with an air
exhaust system and exhaust hose (only original Thermo accessory should be used).
For safety reasons, the installation space should be made of non-combustible materials,
according to DIN 4102.
During installation of built-in units, ensure that the
escaping air will be safely discharged out of the
installation space.
The incubator must only be operated in a location that meets all of the ambient condition
requirements listed below:
• Installation location indoors in dry areas free from drafts.
• The dust burden may not exceed the contamination category 2 based on EN 61010-1.
Using the incubator in an atmosphere with electrically conductive dust is prohibited.
• The minimal distance to adjacent surfaces must be observed on all sides (see section
“Space Requirement” on page 3-4.
• The operating room must be equipped with appropriate ventilation.
• Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the
incubator.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of
the incubator and its accessories (in particular when stacking the table-top units).
• The electrical circuitry of the incubator has been designed for an operating height of up to
2000 m above sea level.
• Relative humidity up to 80% (maximum; preferably 60-70%), non condensing.
• Condensation must be avoided - for example, after moving or transporting the device.
Should condensation exist, wait until the moisture has evaporated completely before
connecting the incubator to a power source and powering up.

Installation
Intermediate Storage
3-2 Heratherm Thermo Scientific
• If a high-voltage test is to be performed on the unit, it must first be heated for around 30
minutes at 75°C.
• The ambient temperature must be within a range of +18 °C to +32 °C (64.4 °F to 89.6 °F).
• Avoid direct exposure to sunlight.
• Devices that produce excessive amounts of heat must not be placed near the incubator.
• Place the incubator on a floor stand (option; to be ordered separately), never on the lab
floor. This prevents the penetration of dust or dirt into the device.
• Power line voltage variations must not exceed ±10 % of the nominal voltage.
• Transient surges must lie within the range of levels that normally occur in the power
supply system. The impulse withstand voltage based on surge category II of IEC 60364-4-
443 shall be applied at the nominal voltage level.
• Consider installing one dedicated upstream circuit breaker per incubator to avoid multiple
device failures in case of an electrical fault.
Contamination hazard
Do not place the incubator directly on the lab floor, but mount it
on the floor stand or on a lab work surface (option; to be ordered
separately). Contaminants, such as bacteria, viruses, fungi,
prions, and other biological substances may use the open door to
migrate easily from the floor into the incubator’s work space.
Intermediate Storage
When the incubator is placed in intermediate storage, which is permissible for a maximum of
four weeks, make sure that the ambient temperature is between 20 °C to 60 °C (68 °F to
140 °F) and the maximum relative humidity does not exceed 90%, non-condensing.
Room Ventilation
Heat dissipating from the incubator during continuous operation may cause a change in the
room climate.
• Therefore, the incubator must only be installed in rooms with sufficient ventilation.
• Do not install the incubator in room recesses without ventilation.
• When several devices are to be placed in the same room, additional ventilation may have
to be provided as necessary.
• To avoid any impact of the heat dissipated by the incubator on the ambient climate the
room must be vented by means of a laboratory-grade ventilation system that complies
with applicable local and national health and safety regulations and has sufficient capacity.

Installation
Room Ventilation
Thermo Scientific Heratherm 3-3
• If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power supply to mitigate the impact of
overtemperature scenarios.

Installation
Room Ventilation
3-4 Heratherm Thermo Scientific
Space Requirement
For built-in units following clearances should be kept:
A, B, C and D see Operating Instructions of the unit.
E (mm/inch) F (mm/inch) G (mm/inch) H (mm/inch)
100 / 4 50 / 2 80 / 3.2 30 / 1.2
Installation Built-in Units
IGS Clearances, as Fig. 3, without exhaust hose.
IMH/IMH-S Clearances, as Fig. 3, close air port with plug, supplied with the unit.
OGS/OMS Clearances, as Fig. 3, with additional space to operate to air slide, with air exhaust
system and exhaust hose Ø 40 mm (1.58 in)/1.5 m (59 in) (original Thermo
accessory), shortened to required length, installed according to Fig. 1 and 2.
OGH/OGH-S
OMH/OMH-S
Clearances, as Fig. 3, with air exhaust system and exhaust hose Ø 40 mm (1.58 in)/1.5
m (59 in) (original Thermo accessory), shortened to required length, installed accord-
ing to Fig. 1 and 2, operating temperature up to max. 250 °C (482 °F).
After connecting the built-in unit to the electrical
power supply, avoid damaging the power cord, then
sliding into the installation position.
When installing the incubator, make sure that the installation and supply connections remain
freely accessible.
The specified side clearances represent minimum distances.