Thermo Scientific 8000DH Series Operating instructions

User Manual
Series 8000DH
CO2Incubator
Direct Heat with Sterilization Cycle
Operating and Maintenance Manuals 7013540 Rev. 4

Thermo ScientificThermo Scientific
Preface
Direct Heat CO2 Incubator i
Model Sensor* Voltage**
3540 T/C 115
3541 T/C 230
3542 IR 115
3543 IR 230
*T/C is a thermal conductivity sensor.
IR is an infra-red sensor.
**All units are 50/60 Hz.
MANUAL NUMBER 7013540
4 31343/IN-4592 10/22/14 New magnetic door switch (parts list, expl dwg, elec schematics) RoHS ccs
3 29174/IN-4469 3/5/14 Updated solenoid - pgs 8-5 & 9-1 through 9-3 ccs
2 30384/IN-4435 1/7/14 Added SRO ext door part number 1900567 to parts list ccs
1 24851, 24849/IN-3971 1/7/10 CO2 valve connection inlet & inner door switch kits-parts list & drawing 370-200-5 ccs
0 25694/IN-3954 10/26/09 Release level 2 - added spring rod kit (pg. 1-7) ccs
REV ECR/ECN DATE DESCRIPTION By

Thermo Scientificii Direct Heat CO2 Incubator Thermo Scientific
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Warning If the incubator is not used in the manner specified in this operating manual, the protection provided
by the equipment design may be impaired. s
©2007 Thermo Scientific. All rights reserved.

Thermo Scientific Direct Heat CO2 Incubator iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Direct Heat CO2 Incubator Thermo Scientific
Preface
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Thermo Scientific Direct Heat CO2 Incubator vThermo Scientific
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate the model and serial numbers.Aserial tag is located on the unit itself.
•For equipment service or maintenance, or with technical or special application inquiries, contact your local
distributor.
Repairs NOT Covered Under Warranty
•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°Cfor
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
abillable service at your location. Calibration after awarranty repair is covered under the warranty.
•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
•Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
•Repairs to parts or systems resulting from unauthorized unit modifications.
•Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Direct Heat CO2 Incubator viThermo Scientific
Table ofContents
Installation and Start-Up ......................................1-1
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Duct Sheets and Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Access Port Filter & CO2 Sensor Cover Plate . . . . . . . . . . . . . . . . .1-9
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
HEPA Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Electrical Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Filling the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Connecting the CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13
Calibration ..................................................2-1
Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Thermal Conductivity CO2 System . . . . . . . . . . . . . . . . . . . . . . . .2-2
Infrared CO2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Section 1
Section 2

vii Direct Heat CO2 Incubator Thermo Scientific
Configuration ................................................3-1
Turn Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set REPLACE HEPA filter reminder . . . . . . . . . . . . . . . . . . . . . . .3-2
Set Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Enable Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . . . .3-3
Set Low Temp Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-3
Set High CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set Low CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set New Zero Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-5
Enable CO2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-5
Setting a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Set New Span Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-6
Enabling Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . .3-7
Enable RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-7
Alarms ......................................................4-1
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Temp Controller Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
TMP CNTR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Routine Maintenance .........................................5-1
Clean Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Replace Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Replace Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Sterilization Cycle ..........................................5-6
Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Table of Contents
Section 3
Section 4
Section 5

Factory Options ..............................................6-1
Remote Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Specifications ...............................................7-1
Spare Parts ..................................................8-1
Exploded Part Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Electrical Schematics ........................................9-1
Warranty Information ........................................10-1
Direct Heat CO2 Incubator viiiThermo Scientific
Table of Contents
Section 6
Section 8
Section 7
Section 9
Section 10

Direct Heat CO2 Incubator 1-1Thermo Scientific
Section 1 Installation and Start-Up
• Chamber Gas Sample Port - Used for sampling chamber CO2content
using a FYRITE or similar instrument.
• Main Power Switch - Mains Disconnect
• Control Panel - Keypad, displays & indicators (Figure 1-2)
• Leveling Legs - Used to level the unit
• Sterilization Cycle Button - Switch to initiate sterilization cycle
Note The incubators are stackable. Information follows. s
Inner Door
Leveling
Legs (4)
Door Heater
Cable
Control Panel
Chamber Gas
Sample Port
Power
Switch
See Section 5
See
Section 1
Sterilization
Cycle Button
Steri
Cycle
Figure 1-1.Components

1-2 Direct Heat CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
- Mutes the audible alarm.
Alarm Indicator - Light pulses on/off during an alarm condition.
MODE Select Switch - Used to select Run, Setpoints, Calibration and
Configuration modes.
Message Display - Shows system status.
Mode Select Indicators -
RUN: Run Menu
SET: Set Points Menu
CAL: Calibration Menu
CON: Configuration Menu
HEAT Indicator - Lights when heaters are activated.
INJ Indicator - Lights during CO2injection into chamber.
T (°C) Display - Programmable to display temperature. See
Configuration section.
RH (%) Display - Shows percentage of humidity. See Configuration sec-
tion.
CO2(%) Display - Programmable to display CO2percentage. See
Configuration section.
Arrow Keys (Scroll for Parameters) - Increases or decreases number val-
ues, toggles between mode choices.
ENTER - Stores the selected value into unit memory.
Figure 1-2.Control Panel
Power Switch
Mode Selector
Switch
Alarm
Indicator
Silence
Switch Mode Select Indicators
Scroll Arrow Keys
ENTER
button
Active Heat Indicator
Active Injection Indicator
Temperature
in °C
Humidity in %
CO2level in %
Message Display

Direct Heat CO2 Incubator 1-3Thermo Scientific
Section 1
Installation and Start-Up
The Series 8000DH incubator has four basic modes for incubator setup:
Run, Setpoints, Calibration and Configuration.
• Run is the default mode for normal operation.
• Set is used to enter system setpoints for incubator operation.
• Calibration is used to calibrate various system parameters.
• Configuration allows for custom setup of various options.
Table 1-1.Selections Under Each Mode
Keypad Operation
RUN SETPOINT CALIBRATION CONFIGURATION
Default Mode Temperature Temp Offset Audible
Overtemp CO2Cal1New HEPA Timer
CO2IR Cal2Replace HEPA Reminder
RH Cal Access Code
Temp Lo Alarm
Temp Relay
CO2Lo Alarm
CO2Hi Alarm
CO2Relay
CO2Z & S #'s *
RH Lo Alarm
RH Relay
Display Temp
Display RH
1T/C units Base Unit Displays
2IR units Option Unit Displays
*T/C units only

Installing the
Incubator
SCROLL FOR PARAMETERS Arrows: Steps the operator through
parameters of SET, CAL and CON modes. The right arrow goes to the
next parameter, the left arrow returns to the previous parameter.
Up/Down Arrows: Increases/decreases or toggles the parameter value
selected in SET, CAL, and CON modes.
ENTER: Must press this key to save-to-memory all changed values.
Key: Press to mute audible alarm. See Section 4 for alarm ringback
times.
Message Display: Shows system status (Mode) at all times. Shows ‘CLASS
100’ or ‘SYSTEM OK’ during normal operation, or alarm messages if
the system detects an alarm condition (see Section 4, Alarms). The mes-
sage ‘CLASS 100’ is a timing mechanism indicating that, under normal
operating conditions with the HEPA filter installed, the air inside the
chamber meets the Class 100 air cleanliness standard for particulates of
0.5 micron size or larger per cubic foot of air. Further information on
the Class 100 classification of air quality is available from Thermo.
Upper and Lower Displays: The 7 segment upper display shows temp and
RH. The lower display shows CO2and O2.
1. Maintain a minimum three inch clearance behind the incubator for
electrical and gas hook-ups. In addition, a three inch ventilation space
is needed on each side.
2. Locate the unit on a firm level surface capable of supporting the unit
weight of 260 lbs (118kg).
3. Locate the unit away from doors and windows and heating and air
conditioning ducts.
4. Lift the unit by the sides of the cabinet base. Do not attempt to lift it
by the front and back. This places stress on the outer door hinges.
1-4 Direct Heat CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Displays
Keypad Operation
(continued)

Stacking the
Incubators
Warning Install stacked units against a wall or similar structure. s
Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time. s
Warning If the units have been in operation, turn them both off and
disconnect the power source before beginning any service work. s
Two stacking brackets (shown at left) are included in the
parts bag shipped with each incubator.
1. Remove the cover plate securing the door cord from the incubator to
be on top. See Figure 1-4. Disconnect the plug from the connector.
2. Remove the four screws securing the door hinges to the unit. Remove
the door and set it aside.
3. Unscrew the two hole plugs from the top cover of the incubator to be
the bottom of the stack (Figure 1-5).
4. Unscrew and remove the 4 leveling feet from
the unit to be stacked on top and lift it onto
the bottom unit. Align all sides.
Warning This incubator weighs 260 lbs (118kg).
Have sufficient personnel available when lifting.
Lift the unit by the sides of the cabinet base to
avoid placing stress on the outer door hinge. s
Direct Heat CO2 Incubator 1-5Thermo Scientific
Section 1
Installation and Start-Up
Figure 1-4.Cover Plate
Figure 1-6.Stacked
Cover plate and bolt hole
with door connector
Figure 1-3.Stacking Brackets
Figure 1-5.Hole Plug Locations

5. Insert the stacking brackets into the slots at the back of the stacked
units as shown in Figure 1-7.
6. Align the slotted holes in the brackets with the mounting holes on the
back of the top incubator. Secure the brackets with the screws and
washers provided in the parts bag. See Figure 1-8.
7. Thread one 1/4 x 20 bolt and washer
included with the stacking brackets,
into the hole behind the cover plate.
Do not tighten. Refer to Figure 1-9.
8. Remove the cover plate from the same
area on the other side of the top unit.
9. Thread the other 1/4 x 20 bolt and
washer into this hole.
10. Tighten the bolts on both sides.
11. Assemble the door hinges to the unit. Secure with the screws.
12. Plug the door cord into the connector, as previously. Secure the cover
plate.
13. Install the cover plate on the other side of the unit. The stacked
incubators are ready for service.
1. Remove vinyl from shelf channels, duct sheets, and air duct, if present.
2. Using a suitable laboratory disinfectant, clean all interior surfaces.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. s
1-6 Direct Heat CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Figure 1-8.Installed Brackets on Back of Unit
Stacking
bracket
Stacking
bracket
Bottom incubator back
Top incubator back
Top unit
Bottom unit
Figure 1-7.Bracket
into Slot
Cover plate and bolt hole
with door connector
Figure 1-9.Bolt Hole
Preliminary Cleaning
Stacking the
Incubators (cont.)

1. Installed included grommets inot the back
flange of each duct sheet; 6 grommets per
sheet. See Figure 1-10.
2. Install the side ducts with the tabs facing into
the center of the chamber with their slots up.
There are no right side or left side ducts,
simply rotate one of them to fit the opposite
side. Tilt the side ducts as they are placed into
the chamber so the tops fit into the top air
duct, then guide them into the vertical position.
Figure 1-11 shows the side duct as it would be
oriented for the right side of the chamber.
3. Referring to Figure 1-11, note that there is no difference in the left
and right shelf channels.
4. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-12.
5. Figure 1-11 shows one of the channels installed on the right side duct.
Direct Heat CO2 Incubator 1-7Thermo Scientific
Section 1
Installation and Start-Up
Duct Sheet and Shelf
Installation
Side Duct
Side toward side duct
Side toward shelf
Right Side Duct
with Shelf Channel Installed
Shelf Channels
Side duct
tab
Shelf channel
rear slot
Shelf channel
front slot
Side duct
tab
Figure 1-11.Shelf Channels & Side Ducts Figure 1-12.Channels and Slots
Figure 1-10.Grommets
on back of duct sheet

1-8 Direct Heat CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
6. Locate the supplied rod, spring and end pieces; 4 each. Assemble the
spring to the rod by positioning the very end of the spring over the
ridge at the rod. Then press the spring to the opposite side. It should
snap into place. See Figures 1-13 and 1-14.
7. Slide end cap over spring
(Figure 1-15).
Note When installing the rods, the
spring end can be installed on
either side of the chamber. The
upper front rod is high in the
chamber and the upper back rod is
lower to allow access to the access
port filter door. s
8. Install one end of the rod into the appropriate hole in the duct sheet.
9. Compress the spring (under the end cap) to insert the other end of the
rod into the hole in the duct sheet on the opposite side.
10. Figure 1-16 shows the four
rods installed.
Installing Duct Sheets
and Shelves (cont.)
Figure 1-15.Spring End
Figure 1-13.Spring End Figure 1-14.Spring Assemble to Rod
Spring
end
Ridge
Back wall of chamber
Front opening of chamber
Figure 1-16.Four Rods Installed in Chamber

Direct Heat CO2 Incubator 1-9Thermo Scientific
Section 1
Installation ans Start-Up
Air Sample Filter
Installation
1. Locate the opening in the top left corner of the interior chamber.
Remove the tape from the opening on the outside of the unit.
2. Locate the stopper with filter in the hardware bag. Lift the metal port
cover and install the assembly in the opening inside the chamber. See
Figure 1-17.
3. Also in the hardware bag is the CO2sensor cover plate. Install, using
the 1/4-turn fasteners. Refer to Figure 1-17 for the location.
1. Remove the filter from the shipping bag.
2. Install the air sample filter assembly to the black hose barb behind the
top duct.
3. Insert the other end of the filter assembly onto the metal tubing on the
top duct. Refer to the Figure 1-18.
Access Port Filter &
CO2Sensor Cover Plate
Figure 1-17.Filter and Plate
Figure 1-18.Air Sample Filter Installation