Thermo Scientific Steri-Cycle i160 CR User manual

50163206 March 2021
Thermo Scientific
Steri-Cycle i160 CR/
Steri-Cycle i250 CR
CO
2
Incubator
Operating In truction

©2020 Thermo Fisher Scientific Inc. All rights reserved.
Trademarks
Heracell Vios CR , Steri-run , Steri-cycle CR , iCan , THRIVE and Cell locker are registered trademarks of
Thermo Scientific.
Thermo Scientific is a brand of Thermo Fisher Scientific Inc.
All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product
operation. These operating instructions are protected by copyright. Any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this operating instructions manual may change at any time and without any prior notice. All technical
information in this document is for reference purposes only. System configurations and specifications in this docu-
ment supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and as-
sumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use
of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document
shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern
all conflicting information between the two documents.

Thermo Scientific Steri-Cycle i160 / i250 CR 1
T
Table of Contents
Preface .............................................................................................................................................. 1
General Notes.........................................................................................................................................1
Identification of the Device and of the Documentation..........................................................................1
Identification of the Device ...............................................................................................................1
Instruction of the Operating Personnel...................................................................................................1
Applicability of the Instructions..............................................................................................................2
Original Document for Translation ...................................................................................................2
Warranty ................................................................................................................................................2
Requirements for Warranty ...............................................................................................................2
Explanation of Safety Information and Symbols.....................................................................................3
Safety Information and Symbols used in the Operating Instructions ..................................................3
Additional Symbols for Safety Information: .......................................................................................4
Symbols on the Device ......................................................................................................................5
Intended Purpose of the Device..............................................................................................................5
Correct Use .......................................................................................................................................5
Incorrect Use .....................................................................................................................................6
Standards and Directives ........................................................................................................................6
Safety Notes on Gases.............................................................................................................................7
Instruction of the Personnel: ..............................................................................................................7
Safety Notes on Carbon Dioxide (CO2) ............................................................................................7
Safety Notes on Oxygen (O2) ............................................................................................................8
Safety Notes on Nitrogen (N2) ..........................................................................................................8
Chapter 1 Delivery of the Device........................................................................................................ 1-1
Packaging ........................................................................................................................................... 1-1
Examination on Receipt ..................................................................................................................... 1-1
Standard equipment Components Steri-Cycle i160 / i250 CR .......................................................... 1-2
Optional Equipment Components Steri-Cycle i160 / i250 CR.......................................................... 1-2
Optional Equipment Components Steri-Cycle i160 / i250 CR ......................................................... 1-3
Optional Equipment Components Steri-Cycle i160 / i250 CR ......................................................... 1-3
Chapter 2 Installation of the Device ................................................................................................... 2-1
Environmental Conditions ................................................................................................................. 2-1
Requirements: ............................................................................................................................... 2-1
Site Ventilation................................................................................................................................... 2-2
Space Requirements............................................................................................................................ 2-2
Transport ........................................................................................................................................... 2-3
Stacking.............................................................................................................................................. 2-4
Stacking Variants ........................................................................................................................... 2-7

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Steri-Cycle i160 / i250 CR Thermo Scientific
Installing the Support Stand with Castors ...................................................................................... 2-7
Support Stand with Castors and Supporting Feet (160i and 250i Unit) ......................................... 2-8
Installing the Floor Stand, 780 mm ............................................................................................. 2-10
Retrofitting/Modifications................................................................................................................ 2-11
Chapter 3 Description of the Device .................................................................................................. 3-1
Steri-Cycle i160 / i250 CR Front View.............................................................................................. 3-2
Steri-Cycle i160 / i250 CR Back View............................................................................................... 3-4
Safety Devices Steri-Cycle i160 / i250 CR ......................................................................................... 3-4
Workspace Atmosphere ...................................................................................................................... 3-5
Temperature: ................................................................................................................................. 3-5
Relative Humidity: ........................................................................................................................ 3-5
Water Quality Recommendations: ................................................................................................. 3-5
Prefilter .......................................................................................................................................... 3-6
HEPA-Filter and Air Duct ............................................................................................................. 3-6
CO2 Supply: ................................................................................................................................. 3-8
O2 Supply: .................................................................................................................................... 3-8
N2 Supply: .................................................................................................................................... 3-9
Door Switch ..................................................................................................................................... 3-10
Sensor System................................................................................................................................... 3-10
Supply Interface................................................................................................................................ 3-13
Standard Interfaces ...................................................................................................................... 3-13
Gas Connection: .......................................................................................................................... 3-14
USB Interface: ............................................................................................................................. 3-14
4-20 mA Interface: ...................................................................................................................... 3-14
Alarm Contact: ............................................................................................................................ 3-16
Power Supply Connection: .......................................................................................................... 3-16
Workspace Components................................................................................................................... 3-16
Inner Container ........................................................................................................................... 3-16
Glass Door and Optional Gas-Tight Screen ................................................................................. 3-17
Water Reservoir ........................................................................................................................... 3-18
Water Filling .................................................................................................................................... 3-20
Heating System ........................................................................................................................... 3-20
Rear Panel Openings ................................................................................................................... 3-21
Shelf System ................................................................................................................................ 3-22
Electromechanical Door Lock........................................................................................................... 3-23
Chapter 4 Start-up ............................................................................................................................... 4-1
Adapting the Unit to the Ambient Atmosphere ................................................................................. 4-1
Preparing the Workspace.................................................................................................................... 4-1
Installing the “MAX” Fill Level Indicator and the Prefilter ................................................................. 4-2
Installing the HEPA Filter in the Rear Wall Filter Enclosure.............................................................. 4-4
Install the Air Duct............................................................................................................................. 4-4
Installing the HEPA-Filter and the Water Reservoir Cover Panel ....................................................... 4-6
Installing the HEPA Filter in the Rear Panel ...................................................................................... 4-9
Installing the Shelf System.................................................................................................................. 4-9
Installation/Removal of the Support Rail ....................................................................................... 4-9
Shelf Support Installation ............................................................................................................ 4-10
Levelling the Device .................................................................................................................... 4-11

Table of Contents
Thermo Scientific Steri-Cycle i160 / i250 CR 3
Mounting the Split Inserts (Optional) into the Steri-Cycle i250 CR ................................................ 4-11
Gas Connection................................................................................................................................ 4-12
Installing Gas Pressure Hoses ....................................................................................................... 4-12
Gas Connection ...........................................................................................................................4-13
Mains Connection ............................................................................................................................ 4-14
Connecting the USB Interface: ......................................................................................................... 4-15
Connecting the Alarm Contact:........................................................................................................4-15
Connecting the 4-20 mA Interface:...................................................................................................4-17
Chapter Operation ............................................................................................................................ -1
Preparing the Device........................................................................................................................... 5-1
Starting Operation..............................................................................................................................5-2
Water Filling ......................................................................................................................................5-3
Chapter 6 Handling and Control ......................................................................................................... 6-1
Power Switch...................................................................................................................................... 6-2
Operating Panel and Operating Structure........................................................................................... 6-3
Versions without O2/N2 Control .................................................................................................. 6-4
Version with combined CO2/O2/N2 Control (Optional) .............................................................6-5
Structure of the Operating Levels ...................................................................................................6-5
Factory Presettings of the iCan Touchscreen Controls......................................................................6-7
Heat-up Phase of the Control Loop Sensors .......................................................................................6-7
Behavior of Keys during Adjustments ................................................................................................. 6-8
Setting the Temperature Set Value......................................................................................................6-8
Setting the CO2 Set Value..................................................................................................................6-9
Setting the O2 Set Value .................................................................................................................. 6-10
Auto-Start Function.......................................................................................................................... 6-12
Activating the Auto-Start Routine ................................................................................................6-14
Interrupting the Auto-Start Routine ............................................................................................ 6-15
Launching Steri-run..........................................................................................................................6-16
User Configuration...........................................................................................................................6-17
Settings / Setup ............................................................................................................................6-17
Data Logging ............................................................................................................................... 6-27
Options .......................................................................................................................................6-31
Icon Description ..........................................................................................................................6-40
Enabling/Disabling the Keypad Lock ........................................................................................... 6-41
Software Versions ........................................................................................................................6-42
Scaling the Trend Display.................................................................................................................6-42
Error Messages..................................................................................................................................6-44
Response to an Error Message Event ............................................................................................6-44
Resetting Overtemperature Protection ......................................................................................... 6-45
Measures After Power Outage ...................................................................................................... 6-46
Troubleshooting ..........................................................................................................................6-47
Chapter 7 Shut-Down .......................................................................................................................... 7-1
Shutting the Device Down ................................................................................................................. 7-1
Chapter 8 Cleaning and Disinfection ................................................................................................. 8-1
Cleaning ............................................................................................................................................. 8-1

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Steri-Cycle i160 / i250 CR Thermo Scientific
Decontamination Procedures.............................................................................................................. 8-2
Preparing for Disinfection / Steri-run ................................................................................................. 8-2
Wipe/Spray Disinfection of the Workspace ........................................................................................ 8-3
Steri-run Decontamination Routine ................................................................................................... 8-7
Progress of a Steri-run Decontamination Routine: ......................................................................... 8-8
Activate Steri-run ......................................................................................................................... 8-10
Canceling Steri-run ..................................................................................................................... 8-11
Steri-run Interruption due to Error .............................................................................................. 8-12
Completing Steri-run .................................................................................................................. 8-13
Opening the Door after Canceling Steri-run ................................................................................ 8-14
Chapter 9 Maintenance ....................................................................................................................... 9-1
Inspections and Checks ...................................................................................................................... 9-1
Service Intervals.................................................................................................................................. 9-2
Preparing Temperature Calibration .................................................................................................... 9-2
Temperature Calibration Procedure ................................................................................................... 9-3
Preparing the CO2 Calibration .......................................................................................................... 9-5
CO2 Calibration Procedure................................................................................................................ 9-6
Replacing the HEPA Filter in the Workspace..................................................................................... 9-7
Replacing the HEPA Filter in the Rear Wall Filter Enclosure ........................................................... 9-10
Replacing the Gas Inlet Filter ........................................................................................................... 9-10
Device Fusing Replacement.............................................................................................................. 9-11
Replacing the Outer Door Seal......................................................................................................... 9-11
Spare Parts........................................................................................................................................ 9-12
Chapter 10 Waste Disposal............................................................................................................... 10-1
Overview of the Materials Used:....................................................................................................... 10-1
Chapter 11 Specifications................................................................................................................. 11-1
Steri-Cycle i160 CR......................................................................................................................... 11-2
Steri-Cycle i160 CR......................................................................................................................... 11-4
Steri-Cycle i250 CR......................................................................................................................... 11-6
Steri-Cycle i250 CR......................................................................................................................... 11-8
Chapter 12 Data Communication ..................................................................................................... 12-1
USB Interface .............................................................................................................................. 12-1
Installing the USB Port Driver .................................................................................................... 12-2
Structure of the Command Sequences for Data Communication ..................................................... 12-4
Description of Protocol ............................................................................................................... 12-4
Overview of General Parameters (Addresses 0xxx) ............................................................................ 12-5
Reading General Parameters ........................................................................................................ 12-5
Overview of Incubator Parameters (Addresses 2xxx) ......................................................................... 12-5
Reading Basic Parameters ............................................................................................................ 12-6
Reading Internal Function Parameters ......................................................................................... 12-7
Error Memory Structure................................................................................................................... 12-8
Error Memory Data Set Structure Scheme: .................................................................................. 12-9
Overview of the Possible Error Messages in Hex Coding ........................................................... 12-10
Overview of the Possible Error Messages in Bit Coding
General Device Status, Temperature and CO2 Control Loop: ............................................... 12-10

Table of Contents
Thermo Scientific Steri-Cycle i160 / i250 CR
O2 Control Loop and Water Level: ........................................................................................... 12-11
Data Logger Structure.....................................................................................................................12-11
Data Logger Data Set Structure Scheme: ................................................................................... 12-13
Overview of Possible Event Entries in Bit Coding
Overview of Event Entries, Part I: .......................................................................................... 12-14
Overview of Event Entries, Part II: ............................................................................................12-15
Examples of Data Logger Codes ..................................................................................................... 12-15
Functions for Data Logger Queries ............................................................................................12-16
Example of a Code for a a Data Logger Query ...........................................................................12-16
Program Steri-Cycle i160 / i250 CR .............................................................................................. 12-21
Installing the Steri-Cycle i160 CR & Steri-Cycle i250 CR ....................................................... 12-22
Operating the Steri-Cycle i160 CR & Steri-Cycle i250 CR ...................................................... 12-23
Chapter 13 Device Log ...................................................................................................................... 13-1
Chapter 14 Contact Information Thermo Scientific ........................................................................ 14-1
Appendix A Sixfold Gas Tight Screen for Cell Locker .................................................................... A-1
Installation of the Shelving .................................................................................................................A-2

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Steri-Cycle i160 / i250 CR Thermo Scientific

Thermo Scientific Steri-Cycle i160 / i250 CR 1
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Figures
Figure 2-1. Device Dimensions ...............................................................................................................2-2
Figure 2-2. Lift Points.............................................................................................................................2-3
Figure 2-3. Stacking ................................................................................................................................2-4
Figure 2-4. Stacking Adapter and Base Rack with Stacking Elements......................................................2-5
Figure 2-5. Fastening the Adapter Plate to the Lower Unit......................................................................2-5
Figure 2-6. Removing the Screws ............................................................................................................2-5
Figure 2-7. Support Stand with Castors without Supporting Feet (160i Unit) ........................................2-7
Figure 3-1. Steri-Cycle i160 / i250 CR Front View................................................................................3-2
Figure 3-2. Steri-Cycle i160 / i250 CR Back View .................................................................................3-4
Figure 3-3. HEPA-Filter and Airbox .......................................................................................................3-7
Figure 3-4. Air Duct ...............................................................................................................................3-8
Figure 3-5. Door Switch .......................................................................................................................3-10
Figure 3-6. Temperature, O2, CO2 and Humidity Sensors ..................................................................3-11
Figure 3-7. Sensors for Water Level.......................................................................................................3-11
Figure 3-8. Interfaces (Right Side of Control Box) ................................................................................3-13
Figure 3-9. Steri-Cycle i160 / i250 CR with Glass Door ......................................................................3-17
Figure 3-10. Steri-Cycle i160 CR with Threefold Gas-Tight Screen.....................................................3-18
Figure 3-11. Water Reservoir ................................................................................................................3-19
Figure 3-12. Fill and Drain Valve of Water Reservoir............................................................................3-19
Figure 3-13. Fill and Drain Valve Incubator .........................................................................................3-20
Figure 3-14. Refill Funnel.....................................................................................................................3-20
Figure 3-15. Rear Panel Openings.........................................................................................................3-21
Figure 3-16. Shelf System Components ................................................................................................3-22
Figure 3-17. Door Lock Latch and Emergency Release on Underside of Incubator ...............................3-23
Figure 4-1. “MAX” Fill Level Indicator...................................................................................................4-2
Figure 4-2. „MAX“ Fill Level Indicator and Prefilter...............................................................................4-3
Figure 4-3. Assembling the Air Duct.......................................................................................................4-5
Figure 4-4. Air Duct Parts Steri-Cycle i160 CR......................................................................................4-6
Figure 4-5. Assembling the HEPA-Filter and the Airbox.........................................................................4-7
Figure 4-6. Place the Airbox onto the Water Reservoir Cover Panel........................................................4-8
Figure 4-7. Installing the Airbox .............................................................................................................4-8
Figure 4-8. Support Rail Installation/Removal ........................................................................................4-9
Figure 4-9. Shelf Support Installation....................................................................................................4-10

Figures
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Steri-Cycle i160 / i250 CR Thermo Scientific
Figure 4-10. Installation of Split Inserts ................................................................................................4-11
Figure 4-11. Installing Gas Pressure Hoses............................................................................................4-12
Figure 4-12. Gas Connection................................................................................................................4-13
Figure 4-13. Mains Connection ............................................................................................................4-14
Figure 4-14. Connection Example Alarm Contact ................................................................................4-16
Figure 4-15. Pin-out of the 4-20 mA Interface......................................................................................4-17
Figure 5-1. Water Reservoir ....................................................................................................................5-2
Figure 5-2. Fill and Drain Valve Incubator .............................................................................................5-3
Figure 5-3. Refill Funnel.........................................................................................................................5-3
Figure 5-4. “MAX” Fill Level Indicator...................................................................................................5-4
Figure 6-1. Power Switch ........................................................................................................................6-2
Figure 6-2. Main Screen: Touch-Sensitive Screen Areas ..........................................................................6-3
Figure 6-3. iCan Touchscreen without O2/N2 gas supply ....................................................................6-4
Figure 6-4. iCan Touchscreen with Combined Gas Connection...........................................................6-5
Figure 6-5. Menu Structure Overview.....................................................................................................6-6
Figure 6-6. Heat-up Phase Display..........................................................................................................6-7
Figure 6-7. Temperature Display Field and Temperature Selection Menu ..............................................6-8
Figure 6-8. Setting the Temperature Set Value........................................................................................6-9
Figure 6-9. CO2 Display and CO2 Menu ..............................................................................................6-9
Figure 6-10. Setting the CO2 Set Value................................................................................................6-10
Figure 6-11. O2 Display and O2 Menu................................................................................................6-11
Figure 6-12. Setting the O2 Set Value...................................................................................................6-11
Figure 6-13. Activating the Auto-Start Routine.....................................................................................6-14
Figure 6-14. Auto-Start-Status Display Window...................................................................................6-15
Figure 6-15. Interrupting the Auto-Start Routine .................................................................................6-16
Figure 6-16. Error Message Subsequent to Aborted Auto-Start .............................................................6-16
Figure 6-17. User Configuration Menu.................................................................................................6-17
Figure 6-18. Settings/Setup Menu.........................................................................................................6-18
Figure 6-19. Changing the Keypad Lock Code .....................................................................................6-18
Figure 6-20. Changing the Keypad Lock Code .....................................................................................6-19
Figure 6-21. Date/Time Menu..............................................................................................................6-20
Figure 6-22. Setting the Date................................................................................................................6-20
Figure 6-23. Setting the Time ...............................................................................................................6-21
Figure 6-24. Adjusting Display Brightness ............................................................................................6-22
Figure 6-25. Adjusting Key Tone Volume.............................................................................................6-23
Figure 6-26. Interface USB Menu .........................................................................................................6-24
Figure 6-27. USB Interface Baud Rate Setting ......................................................................................6-24
Figure 6-28. Language Setting...............................................................................................................6-25
Figure 6-29. Choose the Desired Reminder Interval Function. .............................................................6-26
Figure 6-30. Setting the Reminder Interval for Steri-run.......................................................................6-27

Figures
Thermo Scientific Steri-Cycle i160 / i250 CR 3
Figure 6-31. Event Logging Menu........................................................................................................ 6-28
Figure 6-32. Event Display................................................................................................................... 6-28
Figure 6-33. Logging Cycle Setting ...................................................................................................... 6-29
Figure 6-34. Displaying the Error Table............................................................................................... 6-31
Figure 6-35. Options Menu ................................................................................................................. 6-32
Figure 6-36. Alarm Menu..................................................................................................................... 6-32
Figure 6-37. Alarm Relay Setting.......................................................................................................... 6-33
Figure 6-38. Low Humidity Setting ..................................................................................................... 6-34
Figure 6-39. Setting the Gas Tight Screen............................................................................................ 6-35
Figure 6-40. Setting the Water Level Sensor......................................................................................... 6-36
Figure 6-41. Setting the Alarm Relay.................................................................................................... 6-37
Figure 6-42. Switching the O2 Control on and off............................................................................... 6-38
Figure 6-43. HEPA Configuration ....................................................................................................... 6-39
Figure 6-44. Activating / De-Activating the HEPA-Filter..................................................................... 6-39
Figure 6-45. Icon Description .............................................................................................................. 6-40
Figure 6-46. Enabling/Disabling the Keypad Lock ............................................................................... 6-42
Figure 6-47. Software Versions............................................................................................................. 6-42
Figure 6-48. Invoking the CO2 Concentration Graph Display ............................................................ 6-43
Figure 6-49. Invoking the CO2 Concentration Graph Display ............................................................ 6-43
Figure 6-50. Event Error Messages ....................................................................................................... 6-45
Figure 6-51. Failure Message Overtemperature..................................................................................... 6-45
Figure 6-52. Failure Message Overtemperature..................................................................................... 6-46
Figure 7-1. Fill and Drain Valve of Water Reservoir............................................................................... 7-1
Figure 8-1. Fill and Drain Valve of Water Reservoir............................................................................... 8-2
Figure 8-2. HEPA-Filter and Airbox ...................................................................................................... 8-5
Figure 8-3. Air Duct............................................................................................................................... 8-6
Figure 8-4. Decontamination Routine Phases......................................................................................... 8-9
Figure 8-5. Steri-run Menu - Progress .................................................................................................. 8-10
Figure 8-6. Activate Steri-run ............................................................................................................... 8-11
Figure 8-7. Interrupting / Canceling Steri-run...................................................................................... 8-12
Figure 8-8. To stop Steri-run................................................................................................................ 8-13
Figure 8-9. Door Lock Latch and Emergency Release on Underside of Incubator ................................ 8-14
Figure 9-1. Preparing Temperature Calibration...................................................................................... 9-3
Figure 9-2. Temperature Display Field and Temperature Selection Menu.............................................. 9-4
Figure 9-3. Temperature Calibration Procedure ..................................................................................... 9-4
Figure 9-4. Gas-Tight Screen Measurement Opening............................................................................. 9-5
Figure 9-5. CO2 Calibration Procedure ................................................................................................. 9-7
Figure 9-6. Removing the Airbox ........................................................................................................... 9-8
Figure 9-7. Installing the HEPA-Filter ................................................................................................... 9-9
Figure 9-8. Gas Inlet Filter Installation................................................................................................. 9-11

Figures
4
Steri-Cycle i160 / i250 CR Thermo Scientific

Thermo Scientific Steri-Cycle i160 / i250 CR 1
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Preface
General Notes
Identification of the Device and of the Documentation
Identification of the Device
Identification data
Device name: CO2 incubator
Unit Name: Steri-Cycle i160 CR
Steri-Cycle i250 CR
Allocation of product documentation
Documents CD-ROM 50163127
Certifications and quality audit:
Certification: CE Certification
Test mark: TÜV GS, cCSAus, EAC
Instruction of the Operating Personnel
These operating instructions describe the CO2 incubator Steri-Cycle i160 / i250 CR. The CO2
incubator has been manufactured in keeping with the latest technological developments and is
operationally safe. However, the device may present potential hazards. Particularly if the device is
operated by inadequately trained personnel or if it is not used in accordance with the intended
purpose. Therefore, the following must be observed to prevent accidents:
• The CO2 incubator must be operated only by trained and authorized personnel.
• For any operation of this device, the operator must prepare clear and concise written instructions
in the language of the operating and cleaning personnel based on these operating instructions,
applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular:
• which decontamination measures are to be applied for the device and accessories,
• which protective measures apply when specific agents are used,
• the measures to be taken in case of accidents,
• which precautions and rules of conduct are necessary when entering and working in a clean room.
• Repairs to the device must be carried out only by trained and authorized expert personnel.

Preface
Applicability of the Instructions
2
Steri-Cycle i160 / i250 CR Thermo Scientific
Applicability of the Instructions
Original Document for Translation
• The contents of the operating instructions are subject to change without further notice.
• In case of conflicting translations into foreign languages the German-language version of these
operating instructions shall be binding.
• Keep these operating instructions close to the device so that safety instructions and important
information are always accessible.
Should you encounter problems that are not detailed adequately in these operating instructions, please
contact Thermo Scientific immediately for your own safety.
Warranty
Requirements for Warranty
Thermo Scientific warrants the operational safety and functions of the CO2 incubator for 2 years, only
under the condition that:
• the device is operated and serviced exclusively in accordance with its intended purpose and as
described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Thermo Fisher Scientific are used,
• inspections and maintenance are performed at the specified intervals.
The Cell Locker and all accessories have a one year warranty. The warranty is valid from the date of
delivery of the device to the operator.

Preface
Explanation of Safety Information and Symbols
Thermo Scientific Steri-Cycle i160 / i250 CR 3
Explanation of Safety Information and Symbols
Safety Information and Symbols used in the Operating Instructions
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injuries.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injuries.
CAUTION Indicates a situation which, if not avoided, could result in property damage.
Note Is used for applicational hints and useful information.

Preface
Explanation of Safety Information and Symbols
4
Steri-Cycle i160 / i250 CR Thermo Scientific
Additional Symbols for Safety Information:
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
Tilting hazard!

Preface
Intended Purpose of the Device
Thermo Scientific Steri-Cycle i160 / i250 CR
Symbols on the Device
Intended Purpose of the Device
Correct Use
This CO2 incubator is designed for preparing and cultivating cell cultures, but it is not to be used for
medical applications or for in-vitro-diagnostics. To that end, a controlled set of physiological ambient
conditions is produced in the workspace of the device by exerting precise control of the following
parameters:
• Temperature
• CO2 content
• O2/N2 content
• Relative humidity
CE conformity mark: confirms conformity according to EU Guidelines
TÜV test mark, 230 V devices
TÜV test mark, 120 V devices, USA/Canada
EAC conformity mark: confirms conformity according to all technical regulations of the Eurasian
customs union (Russia, Kazakhstan and Belarus)
Observe operating instructions!
Hot surfaces!

Preface
Standards and Directives
6
Steri-Cycle i160 / i250 CR Thermo Scientific
Typically, this CO2 incubator has been designed for being installed and operated in the following areas:
• Laboratories for cytobiological and biotechnological experiments of safety levels L1, L2, and L3
• Medical-microbiological laboratories in accordance with DIN EN 12128
• Research labs in clinics and hospitals
This device is intended for professional use only.
This device is only to be operated indoors.
The device may only be operated by a laboratory technician or an instructed person.
A laboratory technician is a person with appropriate technical training or with many years of
experience in the relevant field of work who, on the basis of his or her knowledge and experience, is
able to recognize and avoid dangers that may emanate from laboratory equipment.
An instructed person is a person who has been instructed by a laboratory technician to avoid these hazards.
Incorrect Use
Do not use cell or tissue cultures in the device that are not in accordance with the regulations of safety
levels L1, L2, and L3. Do not use tissues, substances or liquids that:
• are easily ignitable or explosive,
• release vapors that form combustible or explosive mixtures when exposed to air,
• release poisons.
The Cell locker is not for use in medical devices.
Standards and Directives
The device complies with the following standards and guidelines:
• Low Voltage Directive 2014/35/EU
• IEC 61010-1:2010+ AMD 2016, Safety requirements for electrical equipment for measurement,
control and laboratory use - Part 1: General requirements
• IEC 61010-2-010:2019, Safety requirements for electrical equipment for measurement, control
and laboratory use - Part 2-010: Particular requirements for laboratory equipment for the heating
of material
• EMC Directive 2014/30/EU
• IEC 61326-1:2012, Electrical equipment for measurement, control and laboratory use - EMC
requirements, Part 1: General requirements
• IEC 60529:2.1:COR 2018 (IP54)
• FCC 47, Part 15, § 15.107, EMC regulation FCC
• FCC 47. Part 15, § 15.109, EMC regulation FCC
For other countries, the applicable national regulations are binding.

Preface
Safety Notes on Gases
Thermo Scientific Steri-Cycle i160 / i250 CR 7
Safety Notes on Gases
Instruction of the Personnel:
Personnel operating devices with CO2 supply must be instructed about the particularities in the
handling of CO2 before starting their work:
• Correct operation of pressurized gas containers and gas supply systems,
• obligation to report damages and shortcomings in CO2 supply lines,
• Measures to be taken in case of accidents or failures.
These instructions must be repeated at appropriate intervals and must comprise the particular
operating instructions of the gas supplier.
Safety Notes on Carbon Dioxide (CO2)
Since CO2 is rated as a harmful gas, certain safety instructions must be observed when the CO2
incubator is started up and when the device is operated.
Gases
No other gases than those specified in the intended use may be used.
Note Installation work:
Any work on supply lines and pressurized gas containers, cylinders or containers used for storing
CO2 or O2/N2 must only be carried out by expert personnel using the appropriate tools.
Note
It must be ensured that the exposure limits at work place for CO2 and/or O2/N2 are not exceeded.
For the Federal Republic of Germany the TRGS 900 should be observed; other countries may have
different limit values. The country-specific national exposure limits at work place shall be met.
Suffocation hazard
Large amounts of carbon dioxide (CO2) released into the room atmosphere may cause suffocation.
If CO2 is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!

Preface
Safety Notes on Gases
8
Steri-Cycle i160 / i250 CR Thermo Scientific
Safety Notes on Oxygen (O2)
O2 is a gas that promotes combustion and may explode in combination with grease-containing materials.
Safety Notes on Nitrogen (N2)
Nitrogen mixes easily with air. High concentrations of nitrogen reduce the oxygen content in the air.
Oxygen explosion!
Oxygen (O2) may explode in combination with oils, greases, and lubricants. If highly compressed
oxygen comes in contact with grease- or oil-containing substances, the mixture may explode!
Control panel and display window protective foil!
• For cleaning these device components, use only oil- and grease-free lubricants.
Keep all connections and components of the oxygen system free from substances that contain oil,
grease, or lubricant!
Fire hazard!
Released oxygen (O2) promotes combustion. Do not use open flames in the vicinity of
oxygen-operated systems!
• Do not smoke in the vicinity of oxygen systems.
Do not expose the components of an oxygen system to excessive heat.
Suffocation hazard!
Large amounts of nitrogen (N2) released into the room atmosphere may cause suffocation. If N2 is
released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!