Thermo Scientific Heracell 240i User manual

Heracell 150i/240i 50115191_C_01-2018 Thermo Scientific
CO2-Incubator
Heracell®150i/240i
Operating instructions

Heracell 150i/240i 50115191_C_01-2018 Thermo Scientific
© 2018 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific provides this document to its customers with a product purchase to use in
the product operation. This document is copyright protected and any reproduction of the whole or
any part of this document is strictly prohibited, except with the written authorization of Thermo Fish-
er Scientific. The contents of this document are subject to change without notice. Concerning trans-
lations into foreign languages, the English version of these operating instructions is binding.
All technical information in this document is for reference purposes only. System configurations and
specifications in this document is not part of any sales contract between Thermo Fisher Scientific
and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all conflicting information between the two docu-
ments
This regulation does not apply to reproductions for in-plant use.
Trademarks
All trademarks mentioned in this operating instructions are the exclusive property of the respective
manufacturers.
Thermo Fisher Scientific Inc.
168 3rd Avenue
Waltham, MA 02451
USA
.
Caution
When used as a medical device:
Federal law restricts this device to sale by or on the order of a qualified scientist with studies of
medicine, biology or chemistry.
Safety with regard to the protection of persons, the environment and the material under process
largely depends on the conduct of the persons using the equipment.
This operating manual is important for your safety as well as for the setup, installation, use and
maintenance of the equipment!
To avoid errors and any resulting damage, especially personal injuries, be sure to read this man-
ual carefully before using the equipment and follow all instructions.

Heracell 150i/240i 50115191_C_01-2018 3Thermo Scientific
Table of Contents
Chapter 1 General notes................................................................................................ 9
Overview of the Thermo Fisher international sales
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . organizations9
Identification of the device and of the documentation . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Instruction of the operating personnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Applicability of the instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Explanation of safety information and symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Intended purpose of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Standards and directives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Safety notes on gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Operational Safety Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 2 Delivery of the device.................................................................................. 19
Packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Acceptance inspection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Standard equipment components HERACELL® 150i. . . . . . . . . . . . . . . . . . . . . . . . . . 20
Optional equipment components HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Standard equipment components HERACELL® 240i. . . . . . . . . . . . . . . . . . . . . . . . . . 21
Optional equipment components HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Chapter 3 Installation of the device ............................................................................. 23
Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Room ventilation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Stacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Intermediate Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Retrofitting/Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Chapter 4 Description of the device............................................................................. 29
Front view of HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Rear view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Front view HERACELL® 240i. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Rear view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Safety devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Work space atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Sensor system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Supply interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Work space components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Chapter 5 Start-up ...................................................................................................... 57
Let the device adapt to the ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Preparing the work space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Installing the shelf system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Installing the gas humidification
(optional, only for O2/N2-control) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Installing the optional bottle turning devices HERACELL® 240i . . . . . . . . . . . . . . . . . . 62
Installing the optional center strut HERACELL® 240i for split inserts . . . . . . . . . . . . . . 64
Gas connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Heracell 150i/240i 50115191_C_01-2018 4Thermo Scientific
Power supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Connecting the RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Connecting the USB interface (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Connecting the alarm contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Chapter 6 Operation.................................................................................................... 75
Prior to starting operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Preparing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Starting operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Interruption of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Chapter 7 .......................................................Handling and control (iCanTM touchscreen
controller) ......................................................................................................................... 79
Power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Operating panel and operating structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Versions without O2/N2 control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Version with combined CO2/O2/N2 control (optional) . . . . . . . . . . . . . . . . . . . . . . . . . 82
Structure of the operating levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Factory presettings of the iCanTM touchscreen controls . . . . . . . . . . . . . . . . . . . . . . . 84
Heat-up phase of the control loop sensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Event actions for settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Setting the temperature set value
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Setting the CO2 set value. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Setting the O2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Running the contra-con routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
User configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Scaling the trend display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Chapter 8 Shut-down................................................................................................ 132
Shutting the device down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Chapter 9 Cleaning and disinfection.......................................................................... 133
Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Decontamination procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Wipe/spray disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
contra-con decontamination routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Chapter 10 Maintenance .......................................................................................... 145
Inspections and checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Returns for Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Preparing the temperature calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Temperature calibration procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Preparing the CO2 calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
CO2 calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Replacing the gas inlet filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Replacing the device fuses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Replacing the door seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Chapter 11 Disposal ................................................................................................. 159

Heracell 150i/240i 50115191_C_01-2018 5Thermo Scientific
Chapter 12 Technical data........................................................................................ 161
HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Chapter 13 Data communication .............................................................................. 165
Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Structure of the command sequences for data
communication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
Overview of general parameters (addresses 0xxx) . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Overview of incubator parameters (addresses 2xxx) . . . . . . . . . . . . . . . . . . . . . . . . . . 170
Error memory structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
Data logger structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Examples of data logger codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Program HERACELL® 150i / 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Chapter 14 Device log .............................................................................................. 193

Heracell 150i/240i 50115191_C_01-2018 6Thermo Scientific
Figures
Figure 15. Device dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Figure 16. Lift points . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Figure 17. Stacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Figure 18. Front view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Figure 19. Rear view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Figure 20. Looped-through gas supply HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . 32
Figure 21. Front view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Figure 22. Rear view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Figure 23. Looped-through gas supply HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . 36
Figure 24. Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Figure 25. Temperature, CO2 and O2 sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Figure 26. Supply interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Figure 27. Work space components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Figure 28. Water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Figure 29. Rear panel openings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Figure 30. Bottle turning device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Figure 31. Water pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Figure 32. Support rail installation/removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Figure 33. Shelf support installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Figure 34. Installing gas humidification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Figure 35. Installing the bottle turning devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Figure 36. Bottle turning speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Figure 37. Center strut installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Figure 38. Installing gas pressure hoses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Figure 39. Gas connection without gas monitoring system . . . . . . . . . . . . . . . . . . . . . . . 66
Figure 40. Gas connection with gas monitoring system (optional) . . . . . . . . . . . . . . . . . . 67
Figure 41. Combined connection with gas monitoring system (optional) . . . . . . . . . . . . . 68
Figure 42. Power supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Figure 43. Connection example . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Figure 44. Power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Figure 45. Pressure-sensitive areas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Figure 46. iCanTM touchscreen without O2/N2 gas supply . . . . . . . . . . . . . . . . . . . . . . . 81
Figure 47. iCanTM touchscreen with combined gas connection . . . . . . . . . . . . . . . . . . . 82
Figure 48. Menu structure overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Figure 49. Heat-up phase display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Figure 50. Setting the temperature set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Figure 51. Setting the CO2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Figure 52. Setting the O2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Figure 53. Activating the auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Figure 54. Interrupting the auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Figure 55. Changing the code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Figure 56. Date / time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Figure 57. Display contrast setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Figure 58. Key tone setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Figure 59. RS 232 interface baud rate setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Figure 60. USB interface baud rate setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Figure 61. Language setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Figure 62. Reminder interval setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Figure 63. Event display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Figure 64. Logging cycle setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Figure 65. Error table display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Figure 66. Alarm relay setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Figure 67. Low humidity setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Figure 68. Gas tight screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

Heracell 150i/240i 50115191_C_01-2018 7Thermo Scientific
Figure 69. Water level setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Figure 70. Alarm relay setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Figure 71. Bottle turning device speed setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Figure 72. Switching the O2 control on and off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Figure 73. Icon description overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Figure 74. Gas monitoring icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Figure 75. Enabling/disabling the keypad lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Figure 76. Software versions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Figure 77. Trend display scaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Figure 78. Event error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Figure 79. Event error overtemperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Figure 80. Decontamination routine phases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Figure 81. Activating contra-con . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Figure 82. Interrupting / canceling contra-con . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Figure 83. contra-con completion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Figure 84. Preparing the temperature calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
Figure 85. Temperature calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Figure 86. Preparing CO2 calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Figure 87. Gas-tight screen measurement opening . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
Figure 88. CO2 calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Figure 89. Gas inlet filter installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Figure 90. Device fuse replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Figure 91. Magnetic door seal replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158

Heracell 150i/240i 50115191_C_01-2018 8Thermo Scientific

Heracell 150i/240i 50115191_C_01-2018 9Thermo Scientific
1. General notes
1.1 Overview of the Thermo Fisher international sales
organizations
Postal address Germany:
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Enquiries from Germany:
Phone Sales 0800 1 536376
Phone Service 0800 1 112110
Fax Sales/Service 0800 1 112114
E-Mail [email protected]
Enquiries from Europe, Middle East, and Africa:
Phone + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-7474
E-Mail [email protected]
Postal address USA:
Thermo Fisher Scientific Inc.
168 3rd Avenue
Waltham, MA 02451
USA
Enquiries from North America:
Phone [phone]
Fax [phone]
E-Mail [email protected]
Enquiries from Latin America:
Phone [phone]
Fax [phone]
E-Mail [email protected]
Enquiries from Asia Pacific:
Phone [phone]
Fax [phone]
E-Mail [email protected]

Heracell 150i/240i 50115191_C_01-2018 10 Thermo Scientific
1.2 Identification of the device and of the documentation
Identification of the device
Device name: CO2incubator
Type designation: HERACELL®150i
HERACELL®240i
Serial No.: 40830469 or higher
Allocation of product documentation
User information: Operating instructions 50115191
Valid: 01.2018
Certifications and quality audit:
Certification: CE Certification
Test mark: GS by VDE Germany
CSA by VDE Germany
1.3 Instruction of the operating personnel
These operating instructions describe the CO2incubator HERACELL®150i/240i. The CO2incubator
has been manufactured in keeping with the latest technological developments and is operationally
safe. However, the device may present potential hazards, particularly if it is operated by
inadequately trained personnel or if it is not used in accordance with the intended purpose.
Therefore, the following must be observed to prevent accidents:
•TheCO
2incubator must be operated only by trained and authorized personnel.
• For any operation of this device, the operator must prepare clear and concise written
instructions in the language of the operating and cleaning personnel based on these operating
instructions, applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular:
which decontamination measures are to be applied for the CO2incubator and accessories,
which protective measures apply when specific agents are used,
which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and authorized expert personnel.
1.4 Applicability of the instructions
• The contents of the operating instructions are subject to change without further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the device so that safety instructions and important
information are always accessible.
Should you encounter problems that are not detailed adequately in these operating instructions,
please contact Thermo Scientific immediately for your own safety.

Heracell 150i/240i 50115191_C_01-2018 11 Thermo Scientific
1.5 Warranty
Thermo Scientific warrants the operational safety and functions of the CO2incubator only under
the condition that:
• the device is operated and serviced exclusively in accordance with its intended purpose and as
described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Thermo Fisher Scientific
are used,
• inspections and maintenance are performed at the specified intervals.
The warranty is valid from the date of delivery of the device to the operator.
1.6 Explanation of safety information and symbols
Safety information and symbols used in the operating instructions
Additional symbols for safety informations:
WARNUNG
Indicates a hazardous situation which, if not avoided, could result in death or serious injury!
Caution
Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries.
ACHTUNG
Indicates a situation which, if not avoided, could result in property damage.
Hinweis
Is used for applicational hints and useful information.
Wear safety gloves!
Wear safety goggles!
Harmful liquids!

Heracell 150i/240i 50115191_C_01-2018 12 Thermo Scientific
Symbols on the device
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
CE conformity mark: confirms conformity according to EU Guidelines
VDE test mark
Mark of conformity USA/Canada
Observe operating instructions!
Mark of prescription devices!

Heracell 150i/240i 50115191_C_01-2018 13 Thermo Scientific
1.7 Intended purpose of the device
Correct use
Correct use related to a medical device (USA only)
The intended use of this incubator is to provide an environment with
• controlled temperature,
•CO
2,
• elevated humidity,
• an automatic decontamination mode, and
•O
2at suppressed levels,
for the development of ova or embryos at or near body temperature.
Correct use related to a laboratory device (Rest of the world)
The CO2incubator HERACELL® 150i/240i is a laboratory device for preparing and cultivating cell
and tissue cultures. The device allows the simulation of the special physiological ambient
conditions for these cultures due to the exact control of:
• temperature,
•CO
2content,
•O
2/N2content,
• and increased relative humidity.
Fields of Application
HERACELL® 150i/240i has been designed for installation and operation in the following fields of
application:
• Laboratories for cytobiological and biotechnological experiments of safety levels L1, L2, and L3
• Medical-microbiological laboratories in accordance with DIN EN 12128
• Laboratories in the central area of clinics and hospitals
The gases required for the incubator (CO2and / or O2/N2) are supplied to the device from a
separate gas supply system, either from gas cylinders or from a central pressurized gas container.
Caution
When used as a medical device:
Federal law restricts this device to sale by or on the order of a qualified scientist with studies of
medicine, biology or chemistry.

Heracell 150i/240i 50115191_C_01-2018 14 Thermo Scientific
The layout of the gas supply system must ensure that the operating pressure of the gas supply
lines can be set to a range between 0.8 bar (min.) to 1 bar (max.) and that the pressure cannot be
changed.
If the device is equipped with the optional gas monitoring system, up to four devices can be
interconnected in line, independent of the of the gas supply system capacity.
The CO2incubator is suitable for continuous operation.
Incorrect use
Do not use cell or tissue cultures in the device that are not in accordance with the regulations of
safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitable or explosive,
• release vapors that form combustible or explosive mixtures when exposed to air,
• release poisons,
• release dust,
• exhibit exothermic reactions,
• are pyrotechnical substances.
1.8 Standards and directives
The device complies with the following standards and guidelines:
• DIN EN 61010 - 1:2011, DIN EN 61010 - 2 - 010:2015
• Low Voltage Guideline 2014/35/EU
• EMC Guideline 2014/30/EU
• China EEP hazardous substance informations
http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html
The following safety regulations must be observed if the device is operated within the territory of
the Federal Republic of Germany:
• DGUV Guideline 100-500
• TRBS 3145/TRGS 725
• Safety data sheets of the gas suppliers with regard to the particular characteristics of CO2, O2,
and N2.
• Principles of good microbiological proceedings, notice of the trade association of the German
chemical industry.
For other countries, the applicable national regulations are binding.

Heracell 150i/240i 50115191_C_01-2018 15 Thermo Scientific
1.9 Safety notes
• In the case of biological incubators, biological safety with regard to the protection of
persons, its surroundings and the load is heavily dependent on the observance of
the applicable regulations by the persons using the equipment.
• Even then, however, the possibility of hazards, especially health hazards, arising
cannot be ruled out.
• The residual risk depends on the work performed in each individual case.
• To avoid errors and causing damage, especially personal injuries, be sure to
carefully read this manual before putting the equipment into operation, and follow
all instructions.
• Safe and reliable operation of the unit can only be guaranteed if the necessary
inspections, maintenance and repair work are carried out by Thermo Electron LED
GmbH Service Department personnel or by personnel authorized by our company.
• Gas is to be supplied to each unit by means of a pressure reducer with the inlet
pressure set to between 0.8 and max. 1 bar. This setting must not be changed for
safety reasons.
• The place of installation must be thoroughly ventilated in order to expel the gases
escaping around the pressure relief valve to the outside.
• Only original replacement parts authorized by the manufacturer are to be used.
• Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL
ENGINEERINGPRACTICE

Heracell 150i/240i 50115191_C_01-2018 16 Thermo Scientific
1.10 Safety notes on gases
Safety notes on carbon dioxide (CO2)
Since CO2is rated as a harmful gas, certain safety instructions must be observed when the CO2
incubator is started up and when the device is operated.
Safety notes on oxygen (O2)
O2is a gas that promotes combustion and may explode in combination with grease-containing
materials.
Hinweis
Installation work:
Any work to supply lines and pressurized gas containers, cylinders or containers used for storing CO2or
O2/N2must only be carried out by expert personnel using the appropriate tools.
Instruction of the personnel:
Personnel operating devices with CO2supply must be instructed about the particularities in the handling of
CO2before starting their work:
• correct operation of pressurized gas containers and gas supply systems,
• obligation to report damages and shortcomings in CO2supply lines,
• measures to be taken in case of accidents or failures.
These instructions must be repeated at appropriate intervals and must comprise the particular operating
instructions of the gas supplier.
Suffocation hazard!
Large amounts of CO2released into the room atmosphere may cause suffocation.
If CO2is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!
Oxygen explosion!
O2may explode in combination with oils, greases, and lubricants. If highly compressed oxygen
comes in contact with grease- or oil-containing substances, the mixture may explode!
• For cleaning these device components, use only oil- and grease-free lubricants.
• Keep all connections and components of the oxygen system free from substances that
contain oil, grease, or lubricant!

Heracell 150i/240i 50115191_C_01-2018 17 Thermo Scientific
Safety notes on nitrogen (N2)
Nitrogen mixes easily with air. High concentrations of nitrogen reduce the oxygen content in the air.
1.11 Operational Safety Rules
The following rules must be heeded when working with Heracell 150i/240i:
• Observe the sample weight limits specified for your Heracell-incubator as a whole and its
shelving in particular (Seite 161).
• Arrange the samples evenly throughout the work space, making sure not to place them too
closely to the interior walls to ensure a uniform temperature distribution.
• Do not load your CO2-incubator with substances that exceed the capabilities of the available lab
apparatus and Personal Protection Equipment to provide sufficient degrees of protection to
users and third parties (Seite 14).
• Check the door seal once a month for proper sealing performance and possible damage.
• Do not process any samples containing hazardous chemical substances that may be released
into the ambient air through defective seals or may cause corrosion or other defects on parts of
the CO2-incubator.
• The tempering of defined substances or materials with higher moisture content can result in
increased condensation in the chamber. Measures on page 77 must be observed.
Fire hazard!
Released oxygen promotes combustion. Do not use open flames in the vicinity of
oxygen-operated systems!
• Do not smoke in the vicinity of oxygen systems.
• Do not expose the components of an oxygen system to excessive heat.
Suffocation hazard!
Large amounts of N2released into the room atmosphere may cause suffocation.
If N2is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!

Heracell 150i/240i 50115191_C_01-2018 18 Thermo Scientific

Heracell 150i/240i 50115191_C_01-2018 19 Thermo Scientific
Chapter 2 Delivery of the device
2. Delivery of the device
2.1 Packaging
The CO2incubator HERACELL®150i/240i is delivered in a stable packaging box. All packaging
materials can be separated and are reusable:
• Packaging carton: Recycled paper
• Foam elements: Styrofoam (CFC-free)
• Pallet: Untreated wood
• Packaging film: Polyethylene
• Packaging ribbons: Polypropylene
2.2 Acceptance inspection
After the device has been delivered, check the delivery immediately for:
• completeness
• possible damage
If components are missing or damage is found on the device or the packaging, esp. damages caused by
humidity and water, please contact the line-hauler as well as the Technical Support of Thermo Scientific
immediately.
Caution
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere on the device, take all
necessary precautions to protect personnel handling the incubator. For example, have them
wear protective gloves and other personal protection equipment.

Heracell 150i/240i 50115191_C_01-2018 20 Thermo Scientific
Chapter 2 Delivery of the device
2.3 Standard equipment components HERACELL®150i
2.4 Optional equipment components HERACELL®150i
*1) O2sensor with gas humidification set is packed in a separate carton and placed into the work space during transport.
Quantity of the delivered components (in piece)
HERACELL®150i
CO2 incubator or CO2 /O2 incubator
Solid glass door and
continuous shelves
3-door gas tight screen and
continuous shelves
Shelf 3 3
Support rail for shelf 4 4
Shelf supports for shelf 6 6
Insert for pressure compensation opening 1 1
Plug for pipe channel 1 1
Power supply cable 1 1
Connector, potential-free contact 1 1
Spare caps, set 1 1
CO2connecting hose set 1 1
Open-end wrench, 24 mm 1 1
Allen wrench 2 mm for blower wheel 1 1
Allen wrench 3 mm for blower wheel cover 1 1
Operating instructions (CD) 1 1
Summarized safety instructions
Water pump 1 1
Quantity of the delivered components (in piece)
HERACELL®150i
CO2 incubator or CO2 /O2 incubator
Solid glass door and
continuous shelves
3-door gas tight screen and
continuous shelves
O2connecting hose set 1 1
O2sensor with set for gas humidification *1) 1 1
CO2connecting hose set, gas monitoring (optional) 1 1
O2/ N2connecting hose set, gas monitoring
(optional)
11