Thermo Scientific 3554-35A Installation and maintenance instructions

Low Temperature Incubator
Owner/Operator Manual and Parts List
Model No. 3554-35A (115 VAC)
LT1883X4 • 4/2/09 Serial Number:______________________________

Table of Contents
Safety Information ..............................................................................................................................................3
Alert Signals..................................................................................................................................................3
Warning ........................................................................................................................................................3
Caution..........................................................................................................................................................3
Important ......................................................................................................................................................3
Introduction..........................................................................................................................................................4
Specifications ......................................................................................................................................................5
Power Requirements ....................................................................................................................................5
Shipping Weight............................................................................................................................................5
Operating Environment ................................................................................................................................5
Performance Characteristics ........................................................................................................................5
Unpacking and Installation ..................................................................................................................................6
Packing List ..................................................................................................................................................6
Installation ....................................................................................................................................................6
Wall Mounting ..............................................................................................................................................7
Controls and isplays ........................................................................................................................................8
Controls ........................................................................................................................................................8
isplay and Indicators ..................................................................................................................................8
Operation ............................................................................................................................................................9
General Function Checks ............................................................................................................................9
Controller Operation ....................................................................................................................................9
Changing Chamber Temperature Control Setpoint ....................................................................................10
Accessing Controller Menus ......................................................................................................................11
Safety Thermostat ......................................................................................................................................12
Maintenance......................................................................................................................................................13
Cleaning......................................................................................................................................................13
efrosting....................................................................................................................................................13
Calibration ..................................................................................................................................................14
Repair................................................................................................................................................................15
Replacing Temperature Controller, Alarm or S2 ........................................................................................15
Replacing RT Temperature Sensor..........................................................................................................15
Replacing SSR1, K1, K2, or T1..................................................................................................................16
Replacing FAN, S1 or THM1 ......................................................................................................................16
Troubleshooting ................................................................................................................................................17
Replacement Parts............................................................................................................................................18
Wiring iagram..................................................................................................................................................19
Ordering Procedures ........................................................................................................................................20
Warranty ............................................................................................................................................................24

3
Warning
• This manual must be carefully read and thor-
oughly understood before operating the unit, fail-
ure to follow directions or precautionary meas-
ures could result in serious adverse effects.
• This equipment must be used only as specified
in these instructions. If used in a manner other
than as specified, the protection provided by the
equipment may be impaired.
• This equipment is intended for indoor use only.
• This equipment must be earth grounded for safe
operation.
• Maximum ratings of accessories that may be
plugged into convenience outlet: Load current 2
Amps. @ 115VAC, Leakage Current 250 micro
Amps.
Caution
The aluminum evaporator and other portions of these
units should not be exposed to the corrosive effects of
acidic or caustic materials. Extreme care must be exer-
cised if such materials are stored within to prevent voiding
the warranty. This Incubator is NOT suitable for flamma-
ble material storage.
Important
When operating at 100% cooling, the compressor runs
continuously and thus cools continuously. If no manual
defrosting is conducted for over 2 weeks, ice will build
and the unit will not maintain temperature. See
efrosting.
Safety Information
Warn ng
Warnings alert you to a possibility of
personal injury.
Caut on
Cautions alert you to a possibility of
damage to the equipment.
Note
Notes alert you to pertinent facts and
conditions.
Hot Surface
Hot surfaces alert you to a possibility
of personal injury if you come in con-
tact with a surface during use or for a
period of time after use.
Alert Signals
Caut on
Risk of electric shock.

4
Introduction
The Thermo Scientific Lab-Line Low Temperature
Incubator offers laboratories precise temperature control
over the -10°C to 60°C range. Performance meets
requirements for the preservation of vaccines, biologicals,
incubation of bacterial cultures, and determination of bio-
chemical oxygen demand of sewage. The extended
operating temperature range also includes temperatures
associated with drug stability projects, dairy product eval-
uation and entomological studies.
The spacious chamber accommodates up to 300 stan-
dard BO bottles, or similar containers. The 5 reinforced
shelves can handle up to 60 lbs of sample weight each.
Features include:
• 5 Removable shelves
• 6 Molded door shelves
• oor Lock with key
• Microprocessor based temperature controller,
with temperature readout to .1°C
• 3 Cooling control modes: High precision with
cooling, high precision without cooling and Frost
free with variable cooling
• Solid state relay for heater
• Over/ under temperature safety relay and alarm
Over temperature thermostat
• Compressor relay for energy conservation
• Compressor overload relay
• RT temperature probe
• Protected setpoint mode to avoid accidental
change
• Convenience outlet inside chamber, 2 amp
• Interior light with door switch

5
Power Requirements
115V, 8.9A, 60 Hz (Including 2 A for convenience outlet)External
imensions
Height: 70 in (179cm)
Width: 32 in (81cm)
epth: 32.5 in (83cm)
Volume: 20.3 cu ft (0.58m3)
Shelf ata: 5 in chamber, 6 on door, 1 basket
Capacity: 300 BO bottles
Shipping Weight
283 lb. (128 kg)
Operating Environment
Temperature Range: 10°C to 40°C
Humidity Range: 0% to 90% RH
Maximum Altitude: 6600 ft (2 km)
Air clearances: Back, top and sides 3.0" min.
Over-voltage Category II (IEC664)
Pollution egree 2 (IEC664)
Performance Characteristics:
Operating Range: -10° to +60°C
Uniformity: ±0.5°C*
Stability: ±0.2°C*
isplay Readability: 0.1°C
* Per ASTM method E 1292-94, cooling modes 00 and 100
Specifications

6
Unpacking and Installation
Packing List
Item Qty
Low Temperature Incubator 1
Instructions 1
Wall Bracket (with screws) 2
Installation
The Low Temperature Incubator should be installed on a
level, stable floor. Use the adjustable feet to level the
incubator. Locate the unit so that there is at least 3 inch-
es of clearance around the sides, top and back to ensure
sufficient ventilation. To prevent heat build up within the
unit, do not install it where it will be exposed to strong
sunlight, nor near radiators, furnaces, or other sources of
heat. o not install the incubator where flammable or
corrosive atmospheres may exist.
The Low Temperature Incubator is not to be used out-of-
doors or where excessive moisture or contamination
could cause degrading of mechanical or electrical com-
ponents.
1. Unbolt and remove the wooden slats under-
neath unit.
2. Remove all protective tape from doors and
shelves.
3. Clean interior and exterior with warm soap and
water. Shipping tape residue can be removed
using isopropyl alcohol.
4. Uncoil line cord from rear of unit. Connect the
plug to a power outlet that complies with the
electrical requirements specified on the unit's
label and with proper safety ground connection.
If line cord replacement is necessary, use CSA
types: SJ, SJO or SJT, 16 GA, 3 cond. or
equivalent.
Note
If damage is observed, keep the
shipment intact (including the carton
and all packing material) and file a
claim with the final carrier. Remove
shipping box by cutting bands that
attach box to pallet then lift box off.
Note
When cooling is enabled, heat is dis-
sipated on sides of unit resulting in
side temperatures that are warm but
not hazardous.

Wall Mounting
In order to allow the user to improve the stability of the
incubator, two wall mounting brackets are included. These
brackets allow the incubator to be attached to a wall.
1. Attach brackets to the upper corners on the
back of the unit. Use the screws included with
the unit and the pre-drilled holes located near
the top corners. o not drill additional holes
which could result in damage to the unit.
2. Move the unit into position and attach the brack-
ets to a structurally sound wall or other support.
The brackets will ensure that the appropriate 3
inches of clearance from the wall is maintained.
7
INSTALLATION
Mount brackets flush
with top and sides
Back of Unit

8
Controls
Power ON/OFF Sw tch:
Located on back wall of chamber, is used to turn power
ON or OFF to entire unit.
Keys: Located on front panel.
MENU - Used to access controller menus.
SET - Used to display setting.
LINCREASE setting.
MECREASE setting.
isplay and Indicators
DISPLAY: (On front panel)
isplays chamber and set point temperatures in °C and
prompts from menu.
INDICATORS: (On front panel)
DELAY: Lights when delay start timer for compressor is
timing. Will begin timing when power is first applied or
when cooling compressor is turned off.
ALARM: Indicates that chamber temperature is not within
limits and the safety relay is opened.
COOL: Lit when cooling compressor is on.
HEAT: Lights when controller demands heat.
Controls and isplays

Perform the procedure under General Function Checks if
incubator is new or hasnʼt been operated for a long period
of time.
General Function Checks
1. Apply power by placing the Power ON/ OFF
switch (Located inside chamber) to I (ON) posi-
tion. The Controller, on front of door, will display
L, then, 88.8 (to test display), then begins read-
ing actual chamber temperature.
2. After 3 seconds, the alarm LE on the controller
should extinguish and the safety relay will close
applying power to the heater, fan, compressor
relay and convenience outlet.
3. Press and hold MENU key, after 3 sec. the dis-
play will indicate CAL. Release and press
MENU again to display Pct, press SET, display
should indicate 100. Press and release MENU
again to indicate Pro, press SET, display should
indicate 00, press and release MENU to return
to normal control mode. Press SET to display
control setpoint, display should read 25.0. If the
control setpoint or any of the other parameters
aren't set correctly, use the Lor Mkeys while
pressing SET to change the setting.
4. The ELAY LE will be lit for 7 minutes after
power is first applied, the COOL LE will be out
during this delay period. When the ELAY LE
goes out, the COOL LE will light and the com-
pressor relay will close starting the compressor.
5. Allow unit to stabilize for 1 hour at 25°C, to
ensure that all systems are functioning.
Controller Operation
Usually the only controller setting that is necessary to
change is the chamber temperature control setpoint.
Other parameters of the controller may be changed by
accessing them through the menu key (covered later).
Operation
Note
When power is first applied, if dis-
play indicates S instead of L, a dip-
switch setting on the controller must
be changed. Refer to Replacing
Temp Controller to change dipswitch
setting as required.
9

10
Changing Chamber
Temperature Control Setpoint
1. The chamber temperature is continuously dis-
played, unless a key is pressed.
2. To display the setpoint temperature press the
SET key. The setpoint temperature will remain
on the display for 1 sec. after the SET key is
released.
3. To change the setpoint temperature, press and
hold SET while pressing the Lor Mkeys to
change the setting. (Note that when either Lor
Mkeys are first pressed, the display will begin
to change slowly at first, then increase change
rate after a few seconds.)
4. Select the desired chamber setpoint then
release all keys. The controller display will
revert back to actual chamber temperature after
a few seconds. Allow up to an hour for unit to
stabilize at the new setpoint temperature.
After the incubator temperature has stabilized, place sam-
ples into incubator chamber. For best results, arrange
samples evenly throughout the chamber. Liquid samples
should be covered to prevent evaporation and eventual
frost build-up on evaporator coils, particularly when oper-
ating below ambient.
The Low Temperature Incubator has an internal 2 amp.
convenience outlet inside chamber to operate devices
such as: shakers, rotators, photosynthesis lights, record-
ing thermometers etc.
Accessing Controller Menus
The temperature controller has three menu selections that
can be accessed by pressing and holding the MENU key
for 3 sec. To access menu parameter, press and hold the
SET key. To change the parameter, use the Lor Mkeys
CAL Menu- Calibration Menu is used to change the tem-
perature offset value to correct for differences in chamber
OPERATION

temperature and the displayed temperature. Range -5° to
5°C. See Calibration Procedure.
Pct Menu- (Percent Cooling Menu) Used to select one of
three cooling modes:
100% Mode - Compressor runs continuously which pro-
vides high precision control (±.2°C Stability) over entire
temperature range. Requires manual defrosting.
50% Mode - Compressor runs approximately half the
time, which conserves power and also reduces frost
buildup inside chamber. This method decreases the fre-
quency of manual defrosting but provides less tempera-
ture stability (±1.5°C).
00% Mode - Compressor is completely off. This mode is
ideal for incubating samples at temperatures 10°C or
higher above ambient (35°C to 60°C). It provides the
greatest power savings yet still maintains the advertised
stability. efrosting is not required.
Pro Menu- Protection Menu - Used to select the
Protection mode of operation that determines when the
alarm is activated and if the control setpoint can be
changed. Range 0.0 to 0.2.
0.0 Mode - Normal mode that allows the user to change
setpoint. Alarm activates if chamber temperature (cham-
ber) and setpoint temperature (setpoint) are as follows:
Chamber < 15.0°C and chamber < (setpoint -3) or
Chamber > 40.0°C and chamber > (setpoint +3)
While ALARM is activated, the safety relay is opened
interrupting power to the heater, fan, convenience outlet
and compressor. When chamber temperature returns to
acceptable limits, the ALARM will automatically terminate
and power is reapplied. In order to allow changing of set-
point and eliminate nuisance alarms, an alarm by-pass
takes effect when the setpoint is changed or when power
is first applied. The alarm by-pass works as follows:
Present chamber temperature is stored as a nominal
alarm temperature (nominal). If chamber < setpoint then
alarm temperature (alarm) = nominal -3. If chamber >
setpoint then alarm = nominal +3. If chamber should go
beyond alarm or < -13 or > 63 then the ALARM will acti-
vate. This alarm by-pass is active until chamber reaches
setpoint ±1°C.
11
OPERATION

12
0.1 Mode - Same as 0.0 Mode, except that the setpoint
is locked and can't be changed.
0.2 Mode - Setpoint is locked, plus, the ALARM will acti-
vate and the safety relay will open as follows:
chamber < (setpoint -3) or chamber > (setpoint +3)
In order to use this mode of protection, modes 0.0 or 0.1
will have to be used to reach the setpoint temperature.
Once the chamber temperature reaches the setpoint and
is stable, protection mode 0.2 can be set.
Safety Thermostat
As an added safety feature, the incubator has a thermo-
stat in the heater compartment that limits the upper cham-
ber temperature to 65°C in the event that all other con-
trols fail.
OPERATION
Note
If the chamber temperature falls out-
side the setpoint by ±3°C, the
ALARM will activate and safety relay
will interrupt power. This mode can
only be used if the door is to remain
shut and other disturbances don't
effect the chamber temperature.

Cleaning
Immediately clean all spilled materials from the incubator
and wipe dry. If necessary, moisten a cloth with soap and
water and clean inside and out. o not use any harsh
chemical cleaners. o not attempt to clean the incubator
while the unit is plugged into a power source.
efrosting
Periodic defrosting may be necessary depending on
operating temperature, ambient humidity, moisture from
samples and cooling mode. Time between defrosts may
be from 30 days to years.
Suggested efrost Methods
Method I: For control setpoint down to 10°C, this method
is the least disruptive, if samples can withstand tempera-
ture stability of ±1.5°C for 24 hours. Samples may remain
in chamber during this procedure.
1. (Refer to Accessing Controller Menus ) Press
and hold MENU key until CAL is displayed, then
release and press MENU again until Pct is dis-
played.
2. Select 50 in the Pct menu then press MENU key
twice to return control mode.
3. Allow incubator to remain in this mode for about
24 hours or until frost disappears.
4. Use the MENU key to re-establish the previous
selection for Pct (i.e. 100).
Method II:
1. Remove any samples that may be damaged by
temperatures up to 35°C.
2. Refer to Accessing Menu section of manual and
set Pct parameter to 00.
3. Place Setpoint to 35.0°C.
4. Allow temperature to stabilize, for 1 hour.
13
Maintenance
Warn ng
To reduce the risk of electric shock,
disconnect from power source before
servicing. Before re-applying power,
after maintenance is complete, check
to ensure that safety ground is intact
and making a good connection.

5. After 1 hour, remove power from unit. Open
door and wipe up any excess moisture on floor
of chamber with paper towels or sponge.
6. Apply power and set desired operating parame-
ters back into controller. After chamber temper-
ature stabilizes, place samples previously
removed back into unit.
Calibration
If it becomes necessary to calibrate temperature con-
troller, perform the following:
1. Place an accurate temperature measuring
device in the geometric center of the chamber.
2. Set the controller to the desired operating tem-
perature in 100% cooling mode and allow 2
hours stabilization.
3. Note the chamber temperature on the controller
display and the temperature at the geometric
center.
4. Subtract the chamber display reading from the
geometric center reading.
5. Enter into the Calibration Mode as described in
Accessing Menu.
6. Press and hold SET key, using the Lor Mkeys,
set the calibration parameter to the value deter-
mined in step 4.
7. Allow unit to stabilize for 1 hour and check that
controller value and geometric center value
agree ±0.2°C. Repeat calibration if necessary.
14
MAINTENANCE

Replacing Temp. Controller
1. isconnect incubator from power source.
2. Remove front panel bezel by removing four
screws in top and bottom of bezel.
3. Remove four screws that mount controller to
mounting bracket.
4. Note wire colors and positions on TB1 and TB2,
then remove all wires from TB1 and TB2 and old
controller.
5. Locate switch S1 on lower left corner of new
controller and set the switches as follows:
Set S1-1 (A) to on (down).
Set S1-2 (B) to off (up).
6. Attach wires previously removed from old con-
troller to new controller.
7. Mount new controller and bezel, then apply
power.
8. Refer to Calibration section of this manual, to
calibrate new controller.
Replacing RT Temp. Sensor
1. isconnect incubator from power source.
2. Refer to Replacing Temp Controller, but only
remove sensor wires from TB2.
3. Open incubator door and remove screw that
holds Temp Sensor on door shelf bracket.
4. Remove old sensor, then place new sensor on
bracket.
5. Connect new sensor wires to controller then
mount controller and bezel.
6. Apply power then refer to Calibration section.
Repair
15

16
Replacing SSR1, K1, K2 or T1:
1. isconnect incubator for power source.
2. Remove screws from back panel(s) then remove
back panel(s).
3. Locate device to be replaced on bottom panel
then remove mounting screws.
4. Unplug quick connect terminals, noting posi-
tions.
5. Place quick connects on new device and mount
on panel.
6. Replace back panel, then apply power.
7. Refer to General Functional Checks to ensure
that new device is operating.
Replacing FAN, S1 or THM1:
1. isconnect incubator from power source.
2. Remove basket and shelves from chamber
area.
3. Remove screws from heater cover at rear of
chamber.
4. Rotate heater cover to right side of chamber.
5. Fan is located on back wall and S1 and THM1
are on switch panel attached to heater cover.
6. Replace desired part, then coat electrical con-
nections with RTV102 or other electrical insula-
tor.
7. Re-assemble in opposite order as previously
described. Be sure to install gaskets on either
side of heater cover.
8. Apply power, refer to General Functional Checks
to ensure that new device is operating.
REPAIR

17
Symptom Poss ble Cause Poss ble Solut on to Cond t on
Incubator controller isnʼt functioning, Power switch isnʼt ON or no Place Power ON/ OFF switch inside
no lights etc. power to unit. chamber to ON. Check power outlet by
plugging a known good device into it.
Erratic temperature display Frost buildup. Look for frost efrost per instructions. If possible
readings above or below set point. inside chamber around operate in 50% Cooling Mode to avoid
evaporator frost buildup.
Temperature uniformity in chamber Frost buildup or airflow efrost per instruction. If airflow
above specified limit. blocked by samples. blocked by sample containers, remove
some to allow top to bottom airflow.
Temperature readings too high or Controller may need to be Refer to CALIBRATION section of this
too low compared to thermometer. calibrated. manual.
Control goes into power on reset Inadequate line power. Test If line voltage is < 106 VAC while unit is
sequence while operating. Line voltage of outlet to running, then a higher capacity AC line
Incubator while unit is is required.
operating.
Compressor not operating while Compressor stalled and the If line voltage is < 106 VAC while unit is
COOL LE is lit. thermal overloads opens. running, then a higher capacity AC line
Test line voltage of outlet is required.
while operating.
Temperature readings go far Open heater or heater Unplug unit from power then refer to
below setpoint and trigger alarm control relay SSR1. replacing HTR1. Check heater with
at 15°C. OHM meter, about 29 ohms.
Temperature readings go far above efective cooling relay, If compressor isnʼt running while Cool
setpoint and triggers alarm at 40°C compressor or shorted LE is on then replace K2 relay. If
or above. heater SSR1. Check if compressor is running and evaporator
compressor is running, if so isnʼt cold then cooling system needs
touch evaporator in chamber repair. If evaporator is cold then SSR1
it should be cold. may be shorted.
Controller display works but heater, Safety relay K1 not closing. K1 relay may need replaced.
compressor and chamber fan donʼt.
isplay EEE/99.9 Open sensor or sensor Check sensor connection at controller.
connection. Check sensor with ohm meter, 100
ohms at 0°C and 110 ohms at 25°C.
Cabinet temp. above 63°C. If temps inside the chamber seem
Heater held on. excessive, check SSR1 for short
isplay EEE/99.8 Shorted sensor or connection Check sensor connection at controller.
Cabinet temp below -13°C. Check for Heat LE and if lit, check
Heater not working heater circuit including SSR1
isplay EEE/temp Incubator needs defrosted See erratic temperature display readings.
Troubleshooting
1. Temp Limit = Temp (±3°C or more from setpoint) & (> 40°C or <15°C), safety relay opens.

18
Replacement Parts
Descr pt on Part Number
TC1 Controller SPN102214
Sensor, RT SPN88613
SSR1 Relay SPN88616
K1 Safety Relay SPN102260
K2 Comp. Relay SPN102259
S1 Switch SWX143
FAN1 Motor RF1639X33
THM1 Thermostat SPN88612
T1 Transformer TNX135
Techn cal Ass stance 1-800-926-0505
Customer Serv ce 1-800-766-7000
Replacement Parts

19
Wiring iagram

20
Ordering Procedures
Please refer to the Specification Plate for the complete
model number, serial number, and series number when
requesting service, replacement parts or in any corre-
spondence concerning this unit.
All parts listed herein may be ordered from the Thermo
Sc ent f c dealer from whom you purchased this unit or
can be obtained promptly from the factory. When service
or replacement parts are needed we ask that you check
first with your dealer. If the dealer cannot handle your
request, then contact our Customer Service epartment
at 563-556-2241 or 800-553-0039.
Prior to returning any materials, please contact our
Customer Service epartment for a “Return Materials
Authorization” number (RMA). Material returned without
an RMA number will be refused.