Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Nano Pump
User Manual

1200 Series Nano Pump User Manual
Notices
© Agilent Technologies, Inc. 2006
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Manual Part Number
G2226-90010
Edition
Edition 02/06
Printed in Germany
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76337 Waldbronn
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1200 Series Nano Pump User Manual 3
In This Manual…
This manual contains information for using your Nano Pump.
1 Introduction - Around your nano pump
In this chapter you will find an introduction to the nano pump
and some hardware details to identify the parts of the pump
and their function.
2Gettingstarted
In this chapter you will find road maps to help you to start an
analysis with the nano pump. In addition it provides different
ChemStation screens necessary to configure and start an
analysis with the nano pump.
Follow the step by step priming procedure before using the
nano pump in micro mode.
3 Become an expert
In this chapter you will find hints to optimize your nano LC
system to achieve best chromatographic results. It also offers
diagnosis and build-in tests for maintenance features.
Annex Appendix A

4 1200 Series Nano Pump User Manual

1200 Series Nano Pump User Manual 5
Contents
1 Introduction - Around your Nano Pump
Introduction to the nano pump 10
Nano Pump Main Overview 11
Pumphead Overview 12
Flow connections 13
Fittings and ferrules 14
Electrical connections 15
2Gettingstarted
Road maps to start an analysis with the nano pump 18
Road map to configure the nano pump 18
Road map to prepare the nano pump for an analysis 18
Road map to start an analysis 19
Using the ChemStation to Configure your Pump 20
Pump configuration screen 20
Pump auxiliary screen 21
Setup pump parameter screen 23
Priming the pump for best results 28
Manually Priming the Solvent Channels. 28
Purging the Pump 29
3Becominganexpert
Hints for Successful Use of the Capillaries 34
Hints for Successful Use of the Micro Vacuum Degasser 36

6 1200 Series Nano Pump User Manual
Hints for Successful Use of the Nano Pump 37
Hints for Using the Fast Composition Change/Reconditioning 38
Purpose 38
How the Function Works 39
Hints for Successful Use of the Micro Well Plate Sampler 40
Hints for Successful Use of the Column and Switching Valve
Compartment 41
Best Practices for Filters 42
The solvent inlet filters 42
Inline filter 43
Hints for Successful use of Solvents and Mobile Phase 44
Hints for Choosing the Primary Flow 45
Hints to Optimize the Compressibility Compensation Setting 47
Diagnosis screens 49
Test screens 52
EMF Screen 53
A Appendix A
General Safety Information 56
Operation 56
Safety Symbols 58
Lithium Batteries Information 59
Danish Information 59
Radio Interference 60
Test and Measurement 60
Sound Emission 61
Manufacturer’s Declaration 61

1200 Series Nano Pump User Manual 7
Solvent Information 62
Solvents 62
Agilent Technologies on Internet 63
Index 65

8 1200 Series Nano Pump User Manual

9
Agilent 1200 Series Nano Pump
User Manual
Agilent Technologies
1
Introduction - Around your Nano Pump
Introduction to the nano pump 10
Nano Pump Main Overview 11
Pumphead Overview 12
Flow connections 13
Fittings and ferrules 14
Electrical connections 15
In this chapter you will find an introduction to the nano pump and hardware
details to identify the parts of the pump and their function.
For more information on how to install a nano pump in direct injection mode,
refer to the Service Manual G2226-90110 Chapter 1, Installing the Pump.

10 1200 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Introduction to the nano pump
The nano pump consists of two identical pumping units in a single housing. It
generates gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pump A and
pump B. The solvent selection valve allows the pump A output to originate
from either channel A1 or channel A2. The pump B output may originate from
either channel B1 or channel B2.
The primary flow produced by the two pumping units is proportioned in an
electromagnetic proportional valve (EMPV). The remaining column flow is
measured in a mass flow sensitive flow sensor. The measured flow is
compared with the user-entered column flow setpoint. The flow sensor
controls the EMPV current, causing the EMPV to correctly proportion the
column flow. The primary flow in excess of the required column flow volume
is directed to the waste.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run nano LC applications.
The flow range of the nano pump is between 0.1 µl/min and 1 µl/min.

1200 Series Nano Pump User Manual 11
Introduction - Around your Nano Pump 1
Nano Pump Main Overview
Figure 1 Nano pump main overview
1Pumpdrive A 8Main Board
2Damper 9Power Supply
3EMPV 10 Fan
4Outlet Ball Valve A 11 Flow Sensor
5Pumphead A 12 Pumphead B
6Solvent Selection Valve 13 Active Inlet Valve B
7Leak Sensor
8
9
10
11
12
13
1
2
3
4
5
6
7

12 1200 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Pumphead Overview
Figure 2 Pumphead overview
Pumphead assembly G1311-60004
1Outlet ball valve G1312-60012
2Outlet ball valve to piston 2 capillary G1312-67300
3Support ring 5001-3739
4Plunger housing (including springs) G1311-60002
5Sapphire plunger 5063-6586
6Screw M5, 60 mm 0515-2118
7Adapter G1312-23201
8Screw lock 5042-1303
9Pump chamber housing G1311-25200
10 Seal (pack of 2)
Seal (pack of 2), normal phase applications
5063-6589
0905-1420
11 Active inlet valve (complete with cartridge)
Replacement cartridge for active inlet valve
G1312-60010
5062-8562
1
2
3
4
5
6
7
8
9
11
10

1200 Series Nano Pump User Manual 13
Introduction - Around your Nano Pump 1
Flow connections
Figure 3 Flow connections
1Connection tube A/A G1311-67304
2Outlet ball valve to piston 2 capillary A/A G1312-67300
3Mixing capillary A/A G1312-67302
4Restriction capillary A/A G1312-67304
5Damper to Filter capillary A/A 250 µm 130 mm 01090-87308
6Filter to EMPV capillary A/A 170 µm 280 mm G1375-87400
7EMPV to Flow Sensor capillary D/D 25 µm 220 mm G1375-87321
8Flow Sensor to Sampler capillary D/C 25 µm 350 mm G1375-87322
9Flow Sensor to Sampler capillary D/C 25 µm 550 mm G1375-87323
11
22
33
4
567
8

14 1200 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Fittings and ferrules
Table 1 Fittings and Ferrules
Fitting Type Name Description Conditioning Part Number
ASwagelok 1/16” SST fitting,
front and back
ferrule
10/pk 5062-2418
BLite Touch 4/16” SST fitting 10/pk 5063-6593
Lite Touch 1/32” SST ferrule
and lock ring
10/pk 5065-4423
CRheodyne M4 PEEK fitting 6 fitt/2 plug 5065-4410
DFinger Tight Double winged nuts
and
1/32” ferrules
10/pk 5065-4422

1200 Series Nano Pump User Manual 15
Introduction - Around your Nano Pump 1
Electrical connections
Figure 4 Electrical connections
1
2
3
4
5
6
7
8
9
1Configuration switch
2GPIB
3Slot interface board
4RS232
5Analog output
6Remote
7CAN-bus
8Power plug
9Security lever

16 1200 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump

17
Agilent 1200 Series Nano Pump
User Manual
Agilent Technologies
2
Getting started
Road maps to start an analysis with the nano pump 18
Road map to configure the nano pump 18
Road map to prepare the nano pump for an analysis 18
Road map to start an analysis 19
Using the ChemStation to Configure your Pump 20
Pump configuration screen 20
Pump auxiliary screen 21
Setup pump parameter screen 23
Priming the pump for best results 28
Manually Priming the Solvent Channels. 28
Purging the Pump 29

18 1200 Series Nano Pump User Manual
2 Getting started
Road maps to start an analysis with the nano pump
Road map to configure the nano pump
The road map below guides you through all parameters you may need to
configure your nano pump.
Road map to prepare the nano pump for an analysis
The road map below gives you an overview of the necessary steps to prepare
the nano pump before starting an analysis.
Setup the
Primary Flow
page 21
Setup the
Column Flow
Ready Condition
page 21
Setup the
Minimum
Stroke
page 22
Setup the
Solvent
Compressibility
page 22
Setup the
Mixer Volume
page 20
To configure the nano pump
Setup the
Solvent
Calibration
page 26
Start
Here!
NOTE When the pump is used for the first time or has been turned off for a certain time, it is
recommended to purge each channel at 2500 µl/min for 1 minute at least.
Before running the pump in micro mode, run it in normal mode until the system pressure is
stable.

1200 Series Nano Pump User Manual 19
Getting started 2
Road map to start an analysis
The road map below provides an overview of all different parameters you may
need to configure when you run an analysis in micro mode.
Purge
Set the Purge
Time
page 30
Purge
Set the Purge
Flow Rate
page 30
Purge
Start the purge
page 30
2. Normal Mode
Set the Normal
Mode
page 24
1. Purge
Set the Purge
Channel
page 30
Before starting an analysis
with the nano pump
Normal Mode
Set the Column
Flow
page 25
Start
Here!
Normal Mode
Set the Pressure
Limit
page 27
Normal Mode
Set the Solvent
Composition
page 26
Set the
Column Flow
page 25
Set the
Stop Time
page 25
Set the Fast
reconditioning
ON or OFF
page 25
Set the
Solvent
Composition
page 26
Set the
Micro Mode
page 24 Starting an analysis with
the nano pump
Set the
Solvent
Calibration
page 26
Start
Here!
Set the
Pressure Limit
page 27
Set the
Gradient
Timetable
page 27

20 1200 Series Nano Pump User Manual
2 Getting started
Using the ChemStation to Configure your Pump
Pump configuration screen
To get to this screen, select:
Instrument ➜ More Pump ➜ Pump Configuration
Figure 5 Pump configuration screen
Step 2
Step 1
Step 3
Pump configuration
1 Filter volume The filter volume tells the system the delay volume of the filter. Predefined
volumes or custom volumes can be set by the user
Small - 20 µl
Standard - 100 µl
<any number> - 0 to 500 µl
2 Fast composition
change/
reconditioning
Setting Fast composition change/reconditioning to On means that the
system is reconditioned under the ‘fast’ parameter to return to the initial
composition at the end of the analysis and before the post run. The
parameters are Duration and Maximum Pressure.
Reconditioning is performed by purging the system (pump and inj.) During
this process the needle moves into a waste position. See “Hints for Using
the Fast Composition Change/Reconditioning” on page 38
Fast System Flush:
0.0 to 99999.9 min. in steps
of 0.1 min.
Column Reconditioning:
0.0 to 99999.9 min. in steps
of 0.1 min.
3 Mixer volume In the standard configuration, no mixer is installed in the nano pump