Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Nano Pump
User Manual

1200 Series Nano Pump User Manual
Notices
© Agilent Technologies, Inc. 2006
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Manual Part Number
G2226-90010
Edition
Edition 02/06
Printed in Germany
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76337 Waldbronn
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1200 Series Nano Pump User Manual 3
In This Manual…
This manual contains information for using your Nano Pump.
1 Introduction - Around your nano pump
In this chapter you will find an introduction to the nano pump
and some hardware details to identify the parts of the pump
and their function.
2Gettingstarted
In this chapter you will find road maps to help you to start an
analysis with the nano pump. In addition it provides different
ChemStation screens necessary to configure and start an
analysis with the nano pump.
Follow the step by step priming procedure before using the
nano pump in micro mode.
3 Become an expert
In this chapter you will find hints to optimize your nano LC
system to achieve best chromatographic results. It also offers
diagnosis and build-in tests for maintenance features.
Annex Appendix A

4 1200 Series Nano Pump User Manual

1200 Series Nano Pump User Manual 5
Contents
1 Introduction - Around your Nano Pump
Introduction to the nano pump 10
Nano Pump Main Overview 11
Pumphead Overview 12
Flow connections 13
Fittings and ferrules 14
Electrical connections 15
2Gettingstarted
Road maps to start an analysis with the nano pump 18
Road map to configure the nano pump 18
Road map to prepare the nano pump for an analysis 18
Road map to start an analysis 19
Using the ChemStation to Configure your Pump 20
Pump configuration screen 20
Pump auxiliary screen 21
Setup pump parameter screen 23
Priming the pump for best results 28
Manually Priming the Solvent Channels. 28
Purging the Pump 29
3Becominganexpert
Hints for Successful Use of the Capillaries 34
Hints for Successful Use of the Micro Vacuum Degasser 36

6 1200 Series Nano Pump User Manual
Hints for Successful Use of the Nano Pump 37
Hints for Using the Fast Composition Change/Reconditioning 38
Purpose 38
How the Function Works 39
Hints for Successful Use of the Micro Well Plate Sampler 40
Hints for Successful Use of the Column and Switching Valve
Compartment 41
Best Practices for Filters 42
The solvent inlet filters 42
Inline filter 43
Hints for Successful use of Solvents and Mobile Phase 44
Hints for Choosing the Primary Flow 45
Hints to Optimize the Compressibility Compensation Setting 47
Diagnosis screens 49
Test screens 52
EMF Screen 53
A Appendix A
General Safety Information 56
Operation 56
Safety Symbols 58
Lithium Batteries Information 59
Danish Information 59
Radio Interference 60
Test and Measurement 60
Sound Emission 61
Manufacturer’s Declaration 61

1200 Series Nano Pump User Manual 7
Solvent Information 62
Solvents 62
Agilent Technologies on Internet 63
Index 65

8 1200 Series Nano Pump User Manual

9
Agilent 1200 Series Nano Pump
User Manual
Agilent Technologies
1
Introduction - Around your Nano Pump
Introduction to the nano pump 10
Nano Pump Main Overview 11
Pumphead Overview 12
Flow connections 13
Fittings and ferrules 14
Electrical connections 15
In this chapter you will find an introduction to the nano pump and hardware
details to identify the parts of the pump and their function.
For more information on how to install a nano pump in direct injection mode,
refer to the Service Manual G2226-90110 Chapter 1, Installing the Pump.

10 1200 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Introduction to the nano pump
The nano pump consists of two identical pumping units in a single housing. It
generates gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pump A and
pump B. The solvent selection valve allows the pump A output to originate
from either channel A1 or channel A2. The pump B output may originate from
either channel B1 or channel B2.
The primary flow produced by the two pumping units is proportioned in an
electromagnetic proportional valve (EMPV). The remaining column flow is
measured in a mass flow sensitive flow sensor. The measured flow is
compared with the user-entered column flow setpoint. The flow sensor
controls the EMPV current, causing the EMPV to correctly proportion the
column flow. The primary flow in excess of the required column flow volume
is directed to the waste.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run nano LC applications.
The flow range of the nano pump is between 0.1 µl/min and 1 µl/min.