Agilent Technologies Infinity II 1290 User manual

Agilent Technologies
Agilent 1290 Infinity II
Flexible Pump
User Manual

Agilent 1290 Infinity II Flexible Pump User Manual
Notices
© Agilent Technologies, Inc. 2014
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G7104-90000 Rev. B
Edition
12/2014
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

Agilent 1290 Infinity II Flexible Pump User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1290 Infinity II Flexible Pump (G7104A).
1 Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3UsingthePump
This chapter explains the operational parameters of the Agilent 1290
Infinity II Flexible Pump.
4 How to Optimize the Performance of Your Module
This chapter gives hints on how to optimize the performance or use
additional devices.
5 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity II
Flexible Pump.

4 Agilent 1290 Infinity II Flexible Pump User Manual
In This Guide...
8 Parts and Materials
This chapter provides information on parts for maintenance and repair.
9 Identifying Cables
This chapter provides information on cables used with the modules.
10 Hardware Information
This chapter describes the pump in more detail on hardware and
electronics.
11 LAN Configuration
This chapter provides information on connecting the module to the
controller software.
12 Appendix
This chapter provides additional information on safety, legal and web.

Agilent 1290 Infinity II Flexible Pump User Manual 5
Contents
Contents
1 Introduction 9
Product Description 10
Features 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
Leak and Waste Handling 19
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3UsingthePump 31
Magnets 32
Turn on/off 33
Status Indicators 34
Best Practices 35
4 How to Optimize the Performance of Your Module 39
Delay Volume and Extra-Column Volume 40
How to Configure the Optimum Delay Volume 41
How to Achieve Higher Resolution 43
Using Solvent Calibration Tables 46
5 Troubleshooting and Diagnostics 47
User Interfaces 48
Agilent Lab Advisor Software 49
6 Error Information 51
What Are Error Messages 53
General Error Messages 54
Pump Error Messages 60

6 Agilent 1290 Infinity II Flexible Pump User Manual
Contents
7 Maintenance 79
Introduction to Maintenance 81
Warnings and Cautions 82
Overview of Maintenance 84
Cleaning the Module 85
Install Fittings and Capillaries 86
Remove and Install Doors 87
Replace the Pressure Sensor 88
Replace the Inlet Weaver 90
Replace the Inlet Valve 92
Replace the Outlet Valve 93
Remove the Jet Weaver 95
Install the Jet Weaver 97
Replace the Seal Wash Pump 99
Replace the Multi-Channel Gradient Valve (MCGV) 100
Release a Stuck Inlet Valve 102
Pump Head Procedures 104
Replace the Multi Purpose Valve 133
Replace Parts of the Multi Purpose Valve 135
Replace the High Pressure Outlet Filter or Filter Frit 136
Replace Parts of the Inline Filter 138
Replace the Module Firmware 140
Prepare the Pump Module for Transport 141
8 Parts and Materials 143
Overview of Main Assemblies 144
Flow Connections 146
Pump Head Assembly Parts 148
Primary Pump Head Parts 150
Secondary Pump Head Parts 152
Multi Purpose Valve 154
Cover Parts 155
Accessory Kit 156
Tool Kit 157
Tools 158

Agilent 1290 Infinity II Flexible Pump User Manual 7
Contents
9 Identifying Cables 161
Cable Overview 162
Analog Cables 164
Remote Cables 166
CAN/LAN Cables 170
Agilent Module to PC 171
USB Cables 172
10 Hardware Information 173
Firmware Description 174
Electrical Connections 177
Interfaces 179
Setting the 8-bit Configuration Switch 184
Early Maintenance Feedback 187
Instrument Layout 188
11 LAN Configuration 189
What You Have To Do First 190
TCP/IP Parameter Configuration 191
Configuration Switch 192
Initialization Mode Selection 193
Dynamic Host Configuration Protocol (DHCP) 197
Link Configuration Selection 200
Automatic configuration with Bootp 201
Manual Configuration 211
PC and User Interface Software Setup Setup 216
12 Appendix 219
General Safety Information 220
Waste Electrical and Electronic Equipment Directive 226
Radio Interference 227
Sound Emission 228
Agilent Technologies on Internet 229

8 Agilent 1290 Infinity II Flexible Pump User Manual
Contents

9
Agilent 1290 Infinity II Flexible Pump User Manual
Agilent Technologies
1
Introduction
Product Description 10
Features 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
Leak and Waste Handling 19
Waste Concept 21
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Product Description
Product Description
The Agilent 1290 Infinity II Flexible Pump improves your efficiency by
combining the performance of a high- pressure mixing UHPLC pump with
the flexibility of a low- pressure mixing UHPLC pump.
The new 1290 Infinity II LC power range has a high instrument efficiency,
allowing you to run any HPLC and UHPLC method. ISET enables you to
transfer existing methods from different instrument modules - current
Agilent systems as well as instruments from other manufacturers.
The Agilent Inlet Weaver mixer, active damping or the optional Agilent
V380 Jet Weaver mixer for additional mixing capacity achieve high
analytical efficiency.
The established multipurpose valve enhances laboratory efficiency by
adding useful functionalities, for example, mixer in/out switch, filter
backflush or automatic purge, and BlendAssist software simplifies your
workflow with accurate buffer/additive blending.
Figure 1 Overview of the Flexible Pump
HiVijh^cY^XVidg
Ejbe]ZVY
B8<K
>caZilZVkZg
EdlZghl^iX]
AZV`YgV^c
EgZhhjgZhZchdg

Agilent 1290 Infinity II Flexible Pump User Manual 11
Introduction 1
Features
Features
•High performance in terms of accuracy and precision for flow and
composition.
•High power range combining high pressure up to 1300 bar and high
analytical flow rates up to 5 mL/min for high chromatographic
performance.
•Integrated high efficiency degasser with low internal volume is based on
PTFE AF technology and has a fast change- over of solvents for purging
and priming the pump.
•High solvent mixing efficiency before reaching the pump head due to
the established Inlet Weaver technology.
•Active damping with independently controllable high resolution pump
drives and firmware- embedded tuning algorithms significantly reduce
ripples and associated UV noise, switchable V380 Jet Weaver for extra
mixing volume.
•A multipurpose valve enables automatic software- embedded
functionalities such as switching the optional mixer in and out, flushing
back the inline filter or automatic purging.
•The unique Intelligent System Emulation Technology (ISET) enables the
emulation of existing Agilent 1100, 1200 and 1260 Infinity Series
instruments, as well as Waters Alliance, Waters H- Class and Shimadzu
Prominence instruments.
•Precise and accurate buffer/additive blending using the new software
feature BlendAssist, implemented in the pump driver.
•Built- in active seal- wash for increased uptime.

12 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Operating Principle
Operating Principle
The pump head comprises two pump chambers in series with independent
high- resolution motion control. A pressure sensor in the flow path
monitors the pressure. The pump control uses this signal for minimizing
the pressure ripple in order to achieve highest flow precision. A stable
flow can be delivered even in case of eventual small internal leaks, which
can be compensated automatically. A heat exchanger between two pump
chambers strongly reduces thermal effects due to solvent compression
under very high pressures.
As solvents are compressed by the pump head and expand further down
the flow path, for example in the column, the volumetric flow is changed
depending on the compressibility of the liquid. Agilent control software
allows specifying pure solvents, pre- mixed solvents and solvent gradients.
Associated Agilent solvent libraries are used by the pump control for
enhanced flow accuracy, which is required for cross- instrument or
cross- system reproducibility and method compatibility.
A high resolution encoder unit is attached to the pump drives, which
divides a single turn into 65000 steps. Each step corresponds to a volume
of about 300 pL, which allows an extremely precise control.

Agilent 1290 Infinity II Flexible Pump User Manual 13
Introduction 1
Operating Principle
Figure 2 The hydraulic path
Ejbe]ZVY
>caZilZVkZg
B8<K
EgZhhjgZhZchdg
Bjai^hVbeaZg
HZValVh]ejbe
9Z\VhhZg
HZValVh]hZchdg
Bjai^ejgedhZ
kVakZ
?Zi
LZVkZg

14 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Positions of the Multi Purpose Valve
The Multi Purpose Valve allows easy software controlled switching between
different modes of operation.
Normal Operating Mode Without Mixer
In normal operating mode, the flow comes from the pump head, passes
the pressure sensor and arrives at the central port of the Multi Purpose
Valve. The flow passes the inline filter and leaves the valve through port 4
to the system (autosampler etc.).
Figure 3 Valve position in normal operating mode without mixer
From pump
Inline filter
To system

Agilent 1290 Infinity II Flexible Pump User Manual 15
Introduction 1
Positions of the Multi Purpose Valve
Purge Mode
In purge mode, the flow is diverted to the waste container.
Figure 4 Valve position in purge mode
From pump
To waste

16 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Normal Operating Mode With Mixer
In this mode, the flow passes an optional Jet Weaver and the inline filter.
This configuration is recommended for special applications which require
an increased mixing efficiency.
Figure 5 Valve position in normal operating mode with mixer
From pump
To system
Inline filter
Jet Weaver

Agilent 1290 Infinity II Flexible Pump User Manual 17
Introduction 1
Positions of the Multi Purpose Valve
Filter Flush Mode
This mode is used for cleaning the inline filter by back-flushing it. The
flow goes to port 5, passes the inline filter in opposite direction and
leaves to the waste through port 7.
Figure 6 Valve position in filter flush mode
From pump
Inline filter
To waste

18 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Service Mode
In service mode, the flow is diverted to port 3, where for example a
restriction capillary can be installed for diagnostic tests.
Figure 7 Valve position in service mode
From pump
Restriction
capillary

Agilent 1290 Infinity II Flexible Pump User Manual 19
Introduction 1
Leak and Waste Handling
Leak and Waste Handling
The 1290 Infinity II Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
Figure 8 Leak and waste handling concept (overview - typical stack configuration as an
example)

20 Agilent 1290 Infinity II Flexible Pump User Manual
1Introduction
Leak and Waste Handling
The solvent cabinet is designed to store a maximum volume of 6 L solvent.
The maximum volume for an individual bottle stored in the solvent cabinet
should not exceed 4 L. For details, see the usage guideline for the Agilent
1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has
been shipped with the solvent cabinet, electronic copies are available on
the Internet).
All leak plane outlets are situated in a consistent position so that all
Infinity and Infinity II modules can be stacked on top of each other. Waste
tubes are guided through a channel on the right hand side of the
instrument, keeping the front access clear from tubes.
The leak plane provides leak management by catching all internal liquid
leaks, guiding them to the leak sensor for leak detection, and passing
them on to the next module below, if the leak sensor fails. The leak sensor
in the leak plane stops the running system as soon as the leak detection
level is reached.
Solvent and condensate is guided through the waste channel into the
waste container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler (condensate)
•from the Seal Wash Sensor
•from the pump's Purge Valve or Multipurpose Valve
The waste tube connected to the leak pan outlet on each of the bottom
instruments guides the solvent to a suitable waste container.