Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Nanoflow Pump
User Manual

1260 Nanoflow Pump User Manual
Notices
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2010
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Manual Part Number
G2226-90012
Edition
06/2010
Printed in Germany
Agilent Technologies
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1260 Nanoflow Pump User Manual 3
Contents
Contents
1 Introduction to the 1260 Infinity Nanoflow Pump 7
Introduction to the Pump 8
Early Maintenance Feetback 16
Instrument Layout 17
Electrical Connections 18
Interfaces 20
Setting the 8-bit Configuration Switch (On-Board LAN) 26
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 38
3 Installing the Module 41
Unpacking the Module 42
Optimizing the Stack Configuration 44
Installing the Pump 49
Connecting Modules and Control Software 52
Flow Connections 54
Get the System Ready for the First Injection 58
4UsingthePump 61
Hints For Successfully Using the Pump 62
Solvent Information 64
Algae Growth in HPLC Systems 65
Checkout procedure for a G2229A Nano LC System 67
5 Optimizing Performance 71
Hints for the Micro Vacuum Degasser 72
Choosing the Right Pump Seals 73
How to Choose the Primary Flow 74
The Standard Filter 75
How to Optimize the Compressibility Compensation Setting 76

4 1260 Nanoflow Pump User Manual
Contents
6 Troubleshooting and Diagnostics 79
Overview of the Module’s Indicators and Test Functions 80
Status Indicators 82
User Interfaces 84
Agilent Lab Advisor Software 85
7 Error Information 87
What Are Error Messages 89
General Error Messages 90
Module Error Messages 95
8 Test Functions and Calibration 109
Micro Mode Pressure Test 110
Normal Mode Pressure Test 112
Leak Test 116
EMPV Test 124
EMPV Cleaning 125
9 Maintenance 127
Introduction to Maintenance 128
Warnings and Cautions 129
Overview of Maintenance 131
Cleaning the Module 133
Early Maintenance Feedback (EMF) 134
Checking and Cleaning the Solvent Inlet Filters 136
Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 138
Exchanging the Outlet Valve Sieve 142
Exchanging the Solvent Selection Valve 144
Removing and Disassembling the Pump Head 146
Exchanging the Pump Seals and Seal Wear-in Procedure 148
Exchanging the Pistons 151
Exchanging the Flow Sensor 153
Reassembling the Pump Head Assembly 154
Exchanging the Optional Interface Board 156
Replacing Module Firmware 157

1260 Nanoflow Pump User Manual 5
Contents
10 Parts and Materials for Maintenance 159
Pump Housing and Main Assemblies 160
Solvent Cabinet and Bottle-Head Assembly 163
Hydraulic Path 164
Pump-Head Assembly 166
Flow Sensor Assembly 168
Nanoflow Pump Accessory Kit 169
11 Identifying Cables 171
Cable Overview 172
Analog Cables 174
Remote Cables 176
BCD Cables 179
CAN/LAN Cables 181
External Contact Cable 182
Agilent Module to PC 183
Agilent 1200 Module to Printer 184
12 Appendix 185
General Safety Information 186
The Waste Electrical and Electronic Equipment Directive 189
Batteries Information 190
Radio Interference 191
Sound Emission 192
Solvent Information 193
Agilent Technologies on Internet 194

6 1260 Nanoflow Pump User Manual
Contents

7
1260 Nanoflow Pump User Manual
Agilent Technologies
1
Introduction to the 1260 Infinity
Nanoflow Pump
Introduction to the Pump 8
Hydraulic Path Overview 10
How Does the Pumping Unit Work? 12
How Does Compressibility Compensation Work? 14
How Does Variable Stroke Volume Work? 15
Early Maintenance Feetback 16
Instrument Layout 17
Electrical Connections 18
Serial Number Information (ALL) 18
Rear View of the Module 19
Interfaces 20
Overview Interfaces 22
Setting the 8-bit Configuration Switch (On-Board LAN) 26
Communication Settings for RS-232C 29
Special Settings 31
This chapter provides an introduction to the operation principles of the
nanoflow pump and the electrical interfaces.

8 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
Introduction to the Pump
The low flow pumps consist of two identical pumping units in a single housing.
They generate gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pumphead A
and B. The solvent selection valve allows the pumphead A output to originate
from either channel A1 or channel A2. The pumphead B output may originate
from either channel B1 or channel B2.
In order to deliver fast gradients over the whole composition range, the two
pumpheads deliver a primary flow of 200 – 1100 µL/min from which a mass
flow sensor controlled electromagnetic proportioning valve (EMPV) splits the
set column flow. Excessive solvent is diverted to waste. This electronic flow
control automatically compensates for changes in solvent properties and
backpressure throughout a run.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run capillary/nano LC applications.

1260 Nanoflow Pump User Manual 9
Introduction to the 1260 Infinity Nanoflow Pump 1
Introduction to the Pump
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10 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
Hydraulic Path Overview
The NanoFlow Pump is based on the 1200 Series Binary Pump (pressure limit
400 bar, active inlet valves), and performs all the functions necessary for a
µ-flow solvent delivery system. Basically, these functions are:
•Low pressure metering and high pressure delivery
•Solvent compressibility compensation
•Variable stroke volume
•Column flow measurement and control
Low pressure solvent metering and high pressure solvent delivery are
accomplished by two pump channels, each capable of delivering a maximum of
2.5 mL/min flow at up to 400 bar pressure.
Each channel consists of an identical, independently controlled pump unit.
Each pump unit includes a metering drive assembly and pump head assembly.
The pumphead assemblies both consist of two identical chambers, pistons and
seals, plus an active inlet valve and an outlet valve.
The channel flow outputs are initially joined by a low volume pre-mixer, and
are then connected by a capillary coil to a pressure pulse damper. The
pressure pulse damper also serves as a pressure transducer, which sends
system pressure information to the user interface.
The mixer output flow, called main flow, is connected to the Electronic Flow
Control (EFC) system. The EFC system consists of an Electro-Magnetic
Proportioning Valve (EMPV) in series with a flow sensor. The EMPV is
protected from particles in the mobile phase by a solvent filter frit.
Responding to user-entered column flow setpoint, the EFC system determines
how much of the main flow volume is ultimately delivered to the column. The
remaining main flow volume, which is not required by the column, is diverted
to waste by the EMPV.
Under user control, the EMPV can also function as a purge valve, for purposes
of solvent changeover, etc. In this case, the EMPV is totally open, and the total
main flow is diverted to waste.