Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Nanoflow Pump
User Manual

1260 Nanoflow Pump User Manual
Notices
© Agilent Technologies, Inc. 2007, 2008,
2010
No part of this manual may be reproduced
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tronic storage and retrieval or translation
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Manual Part Number
G2226-90012
Edition
06/2010
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

1260 Nanoflow Pump User Manual 3
Contents
Contents
1 Introduction to the 1260 Infinity Nanoflow Pump 7
Introduction to the Pump 8
Early Maintenance Feetback 16
Instrument Layout 17
Electrical Connections 18
Interfaces 20
Setting the 8-bit Configuration Switch (On-Board LAN) 26
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 38
3 Installing the Module 41
Unpacking the Module 42
Optimizing the Stack Configuration 44
Installing the Pump 49
Connecting Modules and Control Software 52
Flow Connections 54
Get the System Ready for the First Injection 58
4UsingthePump 61
Hints For Successfully Using the Pump 62
Solvent Information 64
Algae Growth in HPLC Systems 65
Checkout procedure for a G2229A Nano LC System 67
5 Optimizing Performance 71
Hints for the Micro Vacuum Degasser 72
Choosing the Right Pump Seals 73
How to Choose the Primary Flow 74
The Standard Filter 75
How to Optimize the Compressibility Compensation Setting 76

4 1260 Nanoflow Pump User Manual
Contents
6 Troubleshooting and Diagnostics 79
Overview of the Module’s Indicators and Test Functions 80
Status Indicators 82
User Interfaces 84
Agilent Lab Advisor Software 85
7 Error Information 87
What Are Error Messages 89
General Error Messages 90
Module Error Messages 95
8 Test Functions and Calibration 109
Micro Mode Pressure Test 110
Normal Mode Pressure Test 112
Leak Test 116
EMPV Test 124
EMPV Cleaning 125
9 Maintenance 127
Introduction to Maintenance 128
Warnings and Cautions 129
Overview of Maintenance 131
Cleaning the Module 133
Early Maintenance Feedback (EMF) 134
Checking and Cleaning the Solvent Inlet Filters 136
Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 138
Exchanging the Outlet Valve Sieve 142
Exchanging the Solvent Selection Valve 144
Removing and Disassembling the Pump Head 146
Exchanging the Pump Seals and Seal Wear-in Procedure 148
Exchanging the Pistons 151
Exchanging the Flow Sensor 153
Reassembling the Pump Head Assembly 154
Exchanging the Optional Interface Board 156
Replacing Module Firmware 157

1260 Nanoflow Pump User Manual 5
Contents
10 Parts and Materials for Maintenance 159
Pump Housing and Main Assemblies 160
Solvent Cabinet and Bottle-Head Assembly 163
Hydraulic Path 164
Pump-Head Assembly 166
Flow Sensor Assembly 168
Nanoflow Pump Accessory Kit 169
11 Identifying Cables 171
Cable Overview 172
Analog Cables 174
Remote Cables 176
BCD Cables 179
CAN/LAN Cables 181
External Contact Cable 182
Agilent Module to PC 183
Agilent 1200 Module to Printer 184
12 Appendix 185
General Safety Information 186
The Waste Electrical and Electronic Equipment Directive 189
Batteries Information 190
Radio Interference 191
Sound Emission 192
Solvent Information 193
Agilent Technologies on Internet 194

6 1260 Nanoflow Pump User Manual
Contents

7
1260 Nanoflow Pump User Manual
Agilent Technologies
1
Introduction to the 1260 Infinity
Nanoflow Pump
Introduction to the Pump 8
Hydraulic Path Overview 10
How Does the Pumping Unit Work? 12
How Does Compressibility Compensation Work? 14
How Does Variable Stroke Volume Work? 15
Early Maintenance Feetback 16
Instrument Layout 17
Electrical Connections 18
Serial Number Information (ALL) 18
Rear View of the Module 19
Interfaces 20
Overview Interfaces 22
Setting the 8-bit Configuration Switch (On-Board LAN) 26
Communication Settings for RS-232C 29
Special Settings 31
This chapter provides an introduction to the operation principles of the
nanoflow pump and the electrical interfaces.

8 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
Introduction to the Pump
The low flow pumps consist of two identical pumping units in a single housing.
They generate gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pumphead A
and B. The solvent selection valve allows the pumphead A output to originate
from either channel A1 or channel A2. The pumphead B output may originate
from either channel B1 or channel B2.
In order to deliver fast gradients over the whole composition range, the two
pumpheads deliver a primary flow of 200 – 1100 µL/min from which a mass
flow sensor controlled electromagnetic proportioning valve (EMPV) splits the
set column flow. Excessive solvent is diverted to waste. This electronic flow
control automatically compensates for changes in solvent properties and
backpressure throughout a run.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run capillary/nano LC applications.

1260 Nanoflow Pump User Manual 9
Introduction to the 1260 Infinity Nanoflow Pump 1
Introduction to the Pump
Figure 1 Overview of the Pump
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10 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
Hydraulic Path Overview
The NanoFlow Pump is based on the 1200 Series Binary Pump (pressure limit
400 bar, active inlet valves), and performs all the functions necessary for a
µ-flow solvent delivery system. Basically, these functions are:
•Low pressure metering and high pressure delivery
•Solvent compressibility compensation
•Variable stroke volume
•Column flow measurement and control
Low pressure solvent metering and high pressure solvent delivery are
accomplished by two pump channels, each capable of delivering a maximum of
2.5 mL/min flow at up to 400 bar pressure.
Each channel consists of an identical, independently controlled pump unit.
Each pump unit includes a metering drive assembly and pump head assembly.
The pumphead assemblies both consist of two identical chambers, pistons and
seals, plus an active inlet valve and an outlet valve.
The channel flow outputs are initially joined by a low volume pre-mixer, and
are then connected by a capillary coil to a pressure pulse damper. The
pressure pulse damper also serves as a pressure transducer, which sends
system pressure information to the user interface.
The mixer output flow, called main flow, is connected to the Electronic Flow
Control (EFC) system. The EFC system consists of an Electro-Magnetic
Proportioning Valve (EMPV) in series with a flow sensor. The EMPV is
protected from particles in the mobile phase by a solvent filter frit.
Responding to user-entered column flow setpoint, the EFC system determines
how much of the main flow volume is ultimately delivered to the column. The
remaining main flow volume, which is not required by the column, is diverted
to waste by the EMPV.
Under user control, the EMPV can also function as a purge valve, for purposes
of solvent changeover, etc. In this case, the EMPV is totally open, and the total
main flow is diverted to waste.

1260 Nanoflow Pump User Manual 11
Introduction to the 1260 Infinity Nanoflow Pump 1
Introduction to the Pump
Figure 2 The Hydraulic Path of the NanoFlow Pump
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12 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
How Does the Pumping Unit Work?
Both pumping units (channel A and channel B) are identical with respect to
parts and function. Each pumping unit consists of a pump head which is
directly attached to a metering drive assembly.
In each metering drive assembly, a servo-controlled variable reluctance motor
and gear train assembly are used to move two ball-screw drives. The gear train
moves the two ball-screw drives in opposite directions (180 degree out of
phase). The gear ratios are designed such that the first ball-screw drive
constantly moves at twice the speed of the second ball-screw drive.
The servo motor includes a high resolution shaft-position encoder, which
continuously reports the speed and direction of the motor in real time. This
speed and direction information is used by the pump control electronics to
ensure precise control of the servo motor movement.
Each pump head consists of two identical chambers, pistons and seals, plus an
active inlet valve and an outlet valve. The solvent volume in each chamber is
displaced by its piston. The pistons are directly moved by the reciprocating
ball-screw drives of the metering drive assembly. Due to the gear design of the
metering drive assembly, the pistons move in opposite directions, with piston
1 constantly moving at twice the speed of piston 2. The outer diameter of the
piston is smaller than the inner diameter of the chamber, allowing solvent to
flow in the gap between the piston and the chamber wall. The two chambers
are connected by the pressure dependent outlet valve.
The position of the solvent selection valve determines which of two solvents
will be sucked (low pressure) through the active inlet valve into chamber 1
during the intake stroke of piston 1. The active inlet valve is electrically
opened and closed, making its operation more precise at low pressures. The
stroke volume of piston 1 is between 2µLand100µL,dependingonflowrate.
When the pump is first turned on, the user is prompted to initialize the pump.
The initialization routine (occurring for both pump heads) first determines the
precise movement limits for both pistons.These limits are then stored in the
pump controller memory. Then, both pistons are set to their default initial
positions.
When pumping begins, the active inlet valve is opened and piston 1 begins its
intake stroke, sucking solvent into chamber 1. At the same time, piston 2
begins its delivery stroke, pumping (high pressure) the existing solvent in
chamber 2 out of the pump head. The pressure produced by piston 2 also

1260 Nanoflow Pump User Manual 13
Introduction to the 1260 Infinity Nanoflow Pump 1
Introduction to the Pump
closes the outlet valve, preventing any chamber 2 solvent from back-streaming
into chamber 1. After a predefined piston 1 stroke length, the servo motor is
stopped, and the active inlet valve is closed. The pistons now reverse
directions. Piston 1 begins its delivery stroke (high pressure), and piston 2
begins its intake stroke. Piston 2 is moving at only half the speed of piston 1.
The outlet valve is forced open by the pressure generated by piston 1. Piston 1
begins to deliver the volume previously sucked into chamber 1. Because of the
2:1 speed ratio of the pistons, half of the solvent flow from chamber 1 is forced
out of the pump head, continuing into the pump hydraulic path. The other half
of the flow from chamber 1 simultaneously refills chamber 2.
When piston 1 has completed its delivery stroke, the pistons reverse direction,
and the cycle is repeated.
Figure 3 Operating Principle of the Pump Head
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14 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Introduction to the Pump
For pump specifications, see “Performance Specifications” on page 38.
How Does Compressibility Compensation Work?
The compressibility of the solvents in use will affect retention-time stability
when the back pressure in the system changes (for example, aging of column).
In order to minimize this effect, the pump provides a compressibility
compensation feature which optimizes the flow stability according to the
solvent type. The compressibility compensation is set to a default value for
each pump head independently. The compensation value for each pump head
can be changed through the user interface.
Without a compressibility compensation the following will happen during a
stroke of the first piston. The pressure in the piston chamber increases and
the volume in the chamber will be compressed depending on backpressure and
solvent type. The volume displaced into the system will be reduced by the
compressed volume.
When a compressibility compensation value for a pump head is set, the pump
processor calculates a compensation volume that depends on the system
pressure and the selected compressibility value. This compensation volume is
added to the delivery stroke of the first piston.
Table 1 Pump Details
Materials in contact with mobile phase
Pump head SST, gold, sapphire, ceramic
Active inlet valve SST, gold, sapphire, ruby, ceramic, PTFE
Outlet valve SST, gold, sapphire, ruby, tantalum
Adapter SST, gold
EMPV SST, ruby, sapphire, PEEK
Flow sensor SST
Damping unit Gold, SST
Capillaries Fused silica

1260 Nanoflow Pump User Manual 15
Introduction to the 1260 Infinity Nanoflow Pump 1
Introduction to the Pump
How Does Variable Stroke Volume Work?
Due to the compression of the pump-chamber volume each piston stroke of the
pump will generate a small pressure pulsation, influencing the flow ripple of
the pump. The amplitude of the pressure pulsation is mainly dependent on the
stroke volume and the compressibility compensation for the solvent in use.
Small stroke volumes will generate less pressure pulsations than higher stroke
volumes at same flow rates. In addition the frequency of the pressure
pulsations will be higher. This will decrease the influence of flow pulsations
on quantitative results.
In gradient mode smaller stroke volumes resulting in less flow ripple will
improve composition ripple.
The pump uses a processor-controlled ball screw system to drive its pistons.
The normal stroke volume is optimized for the selected flow rate. Small flow
rates use a small stroke volume while higher flow rates use a higher stroke
volume.
The stroke volume for the pump is set to AUTO mode. This means that the
stroke is optimized for the flow rate in use. A change to larger stroke volumes
is possible but not recommended.
When the pump is in the standard mode, the EMPV is fully closed. Total main
flow, up to 2500 µL/min, is directed to the LC system. Column flow
measurement/control is disabled. This mode is for non-capillary LC
applications.
In micro mode, the flow sensor measures and controls the column flow in the
range of 0.1 – 4 µL/min.
Flow measurement is based on the principle of mass flow temperature
sensitivity. The flow sensor consists of a heated tube with two temperature
sensors. As the mobile phase passes through the heated tube, the temperature
characteristic distributed over the two temperature sensors is evaluated. From
the temperature characteristic, flow rate accuracy is determined. The flow
sensor measurement is calibrated for specific mobile phases, which are
user-selectable.

16 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Early Maintenance Feetback
Early Maintenance Feetback
Maintenance requires the exchange of components which are subject to wear
or stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of usage of the module and the analytical conditions,
and not on a predefined time interval. The early maintenance feedback (EMF)
feature monitors the usage of specific components in the instrument, and
provides feedback when the user-selectable limits have been exceeded. The
visual feedback in the user interface provides an indication that maintenance
procedures should be scheduled.
EMF Counters
EMF counters increment with use and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded.
Some counters can be reset to zero after the required maintenance procedure.
Using the EMF Counters
The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than
the displayed values) as EMF limits, and then reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.

1260 Nanoflow Pump User Manual 17
Introduction to the 1260 Infinity Nanoflow Pump 1
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers of foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

18 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Electrical Connections
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
•The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
Serial Number Information (ALL)
The serial number information on the instrument labels provide the following
information:
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
CCXZZ00000 Format
CC Country of manufacturing (DE Germany)
X Alphabetic character A-Z (used by manufacturing)
ZZ Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000 Serial number

1260 Nanoflow Pump User Manual 19
Introduction to the 1260 Infinity Nanoflow Pump 1
Electrical Connections
Rear View of the Module
Figure 4 Rear View of the Module
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20 1260 Nanoflow Pump User Manual
1Introduction to the 1260 Infinity Nanoflow Pump
Interfaces
Interfaces
The Agilent 1200 Infinity Series modules provide the following interfaces:
Table 2 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD
(optional)
LAN
(on-board)
RS-232 Analog APG
Remote
Special
Pumps
G1310B Iso Pump
G1311B Quat Pump
G1311C Quat Pump VL
G1312B Bin Pump
G1312C Bin Pump VL
1376A Cap Pump
G2226A Nano Pump
2 Yes No Yes 1 Yes
G4220A/B Bin Pump 2 No Yes Yes No Yes
G1361A Prep Pump 2 Yes No Yes No Yes CAN-DC- OUT for CAN
slaves
Samplers
G1329B ALS
G2260A Prep ALS
2 Yes No Yes No Yes THERMOSTAT for
G1330B
G1364B FC-PS
G1364C FC-AS
G1364D FC-μS
G1367E HiP ALS
G1377A HiP micro ALS
G2258A DL ALS
2 Yes No Yes No Yes THERMOSTAT for
G1330B
CAN-DC- OUT for CAN
slaves
G4226A ALS 2 Yes No Yes No Yes
Detectors
G1314B VWD VL
G1314C VWD VL+
2 Yes No Yes 1 Yes
G1314E/F VWD 2 No Yes Yes 1 Yes