Agilent Technologies 1100 Series User manual

s1
Agilent 1100 Series
Nano Pump
User Manual

ii 1100 Series Nano Pump User Manual
Notices
© Agilent Technologies, Inc. 2002
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G2226-90000
Edition
Edition 07/02
Printed in Germany
Agilent Technologies, Deutschland GmbH
Hewlett-Packard Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
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either express or implied, with regard
to this manual and any information
contained herein, including but not
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ticular purpose. Agilent shall not be
liable for errors or for incidental or
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document that conflict with these
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arate agreement shall control.
Techn ology Li cense s
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Safety Notices
CAUTION
A CAUTION notice denotes a hazard.
It calls attention to an operating pro-
cedure, practice, or the like that, if
not correctly performed or adhered
to, could result in damage to the
product or loss of important data. Do
not proceed beyond a CAUTION
notice until the indicated conditions
are fully understood and met.
WARNING
A WARNING notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
Warning Symbols Used on the
Instrument
The apparatus is marked with this
symbol when the user should refer to
the instruction manual in order to
prevent risk of harm to the operator
and to protect the apparatus against
damage.
!

1100 Series Nano Pump User Manual iii
In This Manual…
This manual contains information for using your Nano Pump.
1 Introduction - Around your nano pump
In this chapter you will find an introduction to the nano pump
and some hardware details to identify the parts of the pump
and their function.
2 Getting started
In this chapter you will find road maps to help you to start an
analysis with the nano pump. In addition it provides different
ChemStation screens necessary to configure and start an
analysis with the nano pump.
Follow the step by step priming procedure before using the
nano pump in micro mode.
3 Become an expert
In this chapter you will find hints to optimize your nano LC
system to achieve best chromatographic results. It also offers
diagnosis and build-in tests for maintenance features.
Annex A Safety Information

iv 1100 Series Nano Pump User Manual

1100 Series Nano Pump User Manual v
Contents
1 Introduction - Around your Nano Pump
Introduction to the nano pump 2
Nano Pump Main Overview 3
Pumphead Overview 4
Flow connections 5
Fittings and ferrules 6
Electrical connections 7
2 Getting started
Road maps to start an analysis with the nano pump 10
Road map to configure the nano pump 10
Road map to prepare the nano pump for an analysis 10
Road map to start an analysis 11
Using the ChemStation to Configure your Pump 12
Pump configuration screen 12
Pump auxiliary screen 13
Setup pump parameter screen 15
Priming the pump for best results 20
Manually Priming the Solvent Channels. 20
Purging the Pump 21
3 Become an expert
Hints for Successful Use of the Capillaries 26
Hints for Successful Use of the Micro Vacuum Degasser 28

vi 1100 Series Nano Pump User Manual
Hints for Successful Use of the Nano Pump 29
Hints for Using the Fast Composition Change/Reconditioning 30
Purpose 30
How the Function Works 30
Hints for Successful Use of the Micro Well Plate Sampler 32
Hints for Successful Use of the Column and Switching Valve
Compartment 33
Best Practices for Filters 34
The solvent inlet filters 34
Inline filter 35
Hints for Successful use of Solvents and Mobile Phase 36
Hints for Choosing the Primary Flow 37
Hints to Optimize the Compressibility Compensation Setting 39
Diagnosis screens 41
Test screens 44
EMF Screen 45
ASafetyInformation
Safety Information 48
General 48
Operation 48
Safety Symbols 49
Lithium Batteries Information 51
Danish Information 51
Radio Interference 52
Test and Measurement 52
Sound Emission 53
Manufacturer’s Declaration 53

1100 Series Nano Pump User Manual vii
Solvent Information 54
Solvents 54
Agilent Technologies on Internet 55
Index 57

viii 1100 Series Nano Pump User Manual

1
Agilent 1100 Series Nano Pump
User Manual
Agilent Technologies
1
Introduction - Around your Nano Pump
Introduction to the nano pump 2
Nano Pump Main Overview 3
Pumphead Overview 4
Flow connections 5
Fittings and ferrules 6
Electrical connections 7
In this chapter you will find an introduction to the nano pump and hardware
details to identify the parts of the pump and their function.
For more information on how to install a nano pump in direct injection mode,
refer to the Service Manual G2226-60100 Chapter 1, Installing the Pump.
For more information on the different stacking possibilities, for a nano pump
included in an online sample enrichment LC/Ion trap MS, refer to the
Orientation Guide G2228-90000.

2 1100 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Introduction to the nano pump
The nano pump consists of two identical pumping units in a single housing. It
generates gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pump A and
pump B. The solvent selection valve allows the pump A output to originate
from either channel A1 or channel A2. The pump B output may originate from
either channel B1 or channel B2.
The primary flow produced by the two pumping units is proportioned in an
electromagnetic proportional valve (EMPV). The remaining column flow is
measured in a mass flow sensitive flow sensor. The measured flow is
compared with the user-entered column flow setpoint. The flow sensor
controls the EMPV current, causing the EMPV to correctly proportion the
column flow. The primary flow in excess of the required column flow volume
is directed to the waste.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run nano LC applications.
The flow range of the nano pump is between 0.1 µl/min and 1 µl/min.

1100 Series Nano Pump User Manual 3
Introduction - Around your Nano Pump 1
Nano Pump Main Overview
Figure 1 Nano pump main overview
1Pumpdrive A 8Main Board
2Damper 9Power Supply
3EMPV 10 Fan
4Outlet Ball Valve A 11 Flow Sensor
5Pumphead A 12 Pumphead B
6Solvent Selection Valve 13 Active Inlet Valve B
7Leak Sensor
8
9
10
11
12
13
1
2
3
4
5
6
7

4 1100 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Pumphead Overview
Figure 2 Pumphead overview
Pumphead assembly G1311-60004
1Outlet ball valve G1312-60012
2Outlet ball valve to piston 2 capillary G1312-67300
3Support ring 5001-3739
4Plunger housing (including springs) G1311-60002
5Sapphire plunger 5063-6586
6Screw M5, 60 mm 0515-2118
7Adapter G1312-23201
8Screw lock 5042-1303
9Pump chamber housing G1311-25200
10 Seal (pack of 2)
Seal (pack of 2), normal phase applications
5063-6589
0905-1420
11 Active inlet valve (complete with cartridge)
Replacement cartridge for active inlet valve
G1312-60010
5062-8562
1
2
3
4
5
6
7
8
9
11
10

1100 Series Nano Pump User Manual 5
Introduction - Around your Nano Pump 1
Flow connections
Figure 3 Flow connections
1Connection tube A/A G1311-67304
2Outlet ball valve to piston 2 capillary A/A G1312-67300
3Mixing capillary A/A G1312-67302
4Restriction capillary A/A G1312-67304
5Damper to Filter capillary A/A 250 µm 130 mm 01090-87308
6Filter to EMPV capillary A/A 170 µm 280 mm G1375-87400
7EMPV to Flow Sensor capillary D/D 25 µm 220 mm G1375-87321
8Flow Sensor to Sampler capillary D/C 25 µm 350 mm G1375-87322
9Flow Sensor to Sampler capillary D/C 25 µm 550 mm G1375-87323
11
22
33
4
567
8

6 1100 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Fittings and ferrules
A Swagelock
Fitting+
front and back ferrule 5062-2418 (10/pk)
BLiteTouch
Fitting
Ferrule and lock ring
5063-6593 (10/pk)
5065-4423 (10/pk)
C Rheodyne PEEK fitting 5065-4410 (6 fit/2 plug)
D Fingertight Double winged nut 5065-4422 (10/pk)

1100 Series Nano Pump User Manual 7
Introduction - Around your Nano Pump 1
Electrical connections
Figure 4 Electrical connections
1
2
3
4
5
6
7
8
9
1Configuration switch
2GPIB
3Slot interface board
4RS232
5Analog output
6Remote
7CAN-bus
8Power plug
9Security lever

8 1100 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump

9
Agilent 1100 Series Nano Pump
User Manual
Agilent Technologies
2
Getting started
Road maps to start an analysis with the nano pump 10
Road map to configure the nano pump 10
Road map to prepare the nano pump for an analysis 10
Road map to start an analysis 11
Using the ChemStation to Configure your Pump 12
Pump configuration screen 12
Pump auxiliary screen 13
Setup pump parameter screen 15
Priming the pump for best results 20
Manually Priming the Solvent Channels. 20
Purging the Pump 21

10 1100 Series Nano Pump User Manual
2 Getting started
Road maps to start an analysis with the nano pump
Road map to configure the nano pump
The road map below guides you through all parameters you may need to
configure your nano pump.
Road map to prepare the nano pump for an analysis
The road map below gives you an overview of the necessary steps to prepare
the nano pump before starting an analysis.
Setup the
Primary Flow
page 13
Setup the
Column Flow
Ready Condition
page 13
Setup the
Minimum
Stroke
page 14
Setup the
Solvent
Compressibility
page 14
Setup the
Mixer Volume
page 12
To configure the nano pump
Setup the
Solvent
Calibration
page 18
Start Here!
NOTE
When the pump is used for the first time or has been turned off for a certain time, it is
recommended to purge each channel at 2500 µl/min for 1 minute at least.
Before running the pump in micro mode, run it in normal mode until the system pressure is
stable.

1100 Series Nano Pump User Manual 11
Getting started 2
Road map to start an analysis
The road map below provides an overview of all different parameters you may
need to configure when you run an analysis in micro mode.
Purge
Set the Purge
Time
page 22
Purge
Set the Purge
Flow Rate
page 22
Purge
Start the purge
page 22
2. Normal Mode
Set the Normal
Mode
page 16
1. Purge
Set the Purge
Channel
page 22
Before starting an analysis
with the nano pump
Normal Mode
Set the Column
Flow
page 17
Start
Here!
Normal Mode
Set the Pressure
Limit
page 19
Normal Mode
Set the Solvent
Composition
page 18
Set the
Column Flow
page 17
Set the
Stop Time
page 17
Set the Fast
reconditioning
ON or OFF
page 17
Set the
Solvent
Composition
page 18
Set the
Micro Mode
page 16 Starting an analysis with
the nano pump
Set the
Solvent
Calibration
page 18
Start
Here!
Set the
Pressure Limit
page 19
Set the
Gradient
Timetable
page 18

12 1100 Series Nano Pump User Manual
2 Getting started
Using the ChemStation to Configure your Pump
Pump configuration screen
To get to this screen, select:
Instrument ➜ More Pump ➜ Pump Configuration
Figure 5 Pump configuration screen
Step 2
Step 1
Step 3
Pump configuration
1 Filter volume The filter volume tells the system the delay volume of the filter. Predefined
volumes or custom volumes can be set by the user
Small - 20 µl
Standard - 100 µl
<any number> - 0 to 500 µl
2 Fast composition
change/
reconditioning
Setting Fast composition change/reconditioning to On means that the
system is reconditioned under the ‘fast’ parameter to return to the initial
composition at the end of the analysis and before the post run. The
parameters are Duration and Maximum Pressure.
Reconditioning is performed by purging the system (pump and inj.) During
this process the needle moves into a waste position. See “Hints for Using
the Fast Composition Change/Reconditioning" on page 30
Fast System Flush:
0.0 to 99999.9 min. in steps
of 0.1 min.
Column Reconditioning:
0.0 to 99999.9 min. in steps
of 0.1 min.
3Mixervolume In the standard configuration, no mixer is installed in the nano pump