Agilent Technologies 1100 Series User manual

s1
Agilent 1100 Series
Nano Pump
User Manual

ii 1100 Series Nano Pump User Manual
Notices
© Agilent Technologies, Inc. 2002
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G2226-90000
Edition
Edition 07/02
Printed in Germany
Agilent Technologies, Deutschland GmbH
Hewlett-Packard Strasse 8
76337 Waldbronn
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arate agreement shall control.
Techn ology Li cense s
The hardware and/or software described in
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Safety Notices
CAUTION
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It calls attention to an operating pro-
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to, could result in damage to the
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ing procedure, practice, or the like
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Instrument
The apparatus is marked with this
symbol when the user should refer to
the instruction manual in order to
prevent risk of harm to the operator
and to protect the apparatus against
damage.
!

1100 Series Nano Pump User Manual iii
In This Manual…
This manual contains information for using your Nano Pump.
1 Introduction - Around your nano pump
In this chapter you will find an introduction to the nano pump
and some hardware details to identify the parts of the pump
and their function.
2 Getting started
In this chapter you will find road maps to help you to start an
analysis with the nano pump. In addition it provides different
ChemStation screens necessary to configure and start an
analysis with the nano pump.
Follow the step by step priming procedure before using the
nano pump in micro mode.
3 Become an expert
In this chapter you will find hints to optimize your nano LC
system to achieve best chromatographic results. It also offers
diagnosis and build-in tests for maintenance features.
Annex A Safety Information

iv 1100 Series Nano Pump User Manual

1100 Series Nano Pump User Manual v
Contents
1 Introduction - Around your Nano Pump
Introduction to the nano pump 2
Nano Pump Main Overview 3
Pumphead Overview 4
Flow connections 5
Fittings and ferrules 6
Electrical connections 7
2 Getting started
Road maps to start an analysis with the nano pump 10
Road map to configure the nano pump 10
Road map to prepare the nano pump for an analysis 10
Road map to start an analysis 11
Using the ChemStation to Configure your Pump 12
Pump configuration screen 12
Pump auxiliary screen 13
Setup pump parameter screen 15
Priming the pump for best results 20
Manually Priming the Solvent Channels. 20
Purging the Pump 21
3 Become an expert
Hints for Successful Use of the Capillaries 26
Hints for Successful Use of the Micro Vacuum Degasser 28

vi 1100 Series Nano Pump User Manual
Hints for Successful Use of the Nano Pump 29
Hints for Using the Fast Composition Change/Reconditioning 30
Purpose 30
How the Function Works 30
Hints for Successful Use of the Micro Well Plate Sampler 32
Hints for Successful Use of the Column and Switching Valve
Compartment 33
Best Practices for Filters 34
The solvent inlet filters 34
Inline filter 35
Hints for Successful use of Solvents and Mobile Phase 36
Hints for Choosing the Primary Flow 37
Hints to Optimize the Compressibility Compensation Setting 39
Diagnosis screens 41
Test screens 44
EMF Screen 45
ASafetyInformation
Safety Information 48
General 48
Operation 48
Safety Symbols 49
Lithium Batteries Information 51
Danish Information 51
Radio Interference 52
Test and Measurement 52
Sound Emission 53
Manufacturer’s Declaration 53

1100 Series Nano Pump User Manual vii
Solvent Information 54
Solvents 54
Agilent Technologies on Internet 55
Index 57

viii 1100 Series Nano Pump User Manual

1
Agilent 1100 Series Nano Pump
User Manual
Agilent Technologies
1
Introduction - Around your Nano Pump
Introduction to the nano pump 2
Nano Pump Main Overview 3
Pumphead Overview 4
Flow connections 5
Fittings and ferrules 6
Electrical connections 7
In this chapter you will find an introduction to the nano pump and hardware
details to identify the parts of the pump and their function.
For more information on how to install a nano pump in direct injection mode,
refer to the Service Manual G2226-60100 Chapter 1, Installing the Pump.
For more information on the different stacking possibilities, for a nano pump
included in an online sample enrichment LC/Ion trap MS, refer to the
Orientation Guide G2228-90000.

2 1100 Series Nano Pump User Manual
1 Introduction - Around your Nano Pump
Introduction to the nano pump
The nano pump consists of two identical pumping units in a single housing. It
generates gradients by high-pressure mixing. A solvent selection valve
provides flexibility in the choice of solvents.
Mobile phase composition is produced by mixing the outputs of pump A and
pump B. The solvent selection valve allows the pump A output to originate
from either channel A1 or channel A2. The pump B output may originate from
either channel B1 or channel B2.
The primary flow produced by the two pumping units is proportioned in an
electromagnetic proportional valve (EMPV). The remaining column flow is
measured in a mass flow sensitive flow sensor. The measured flow is
compared with the user-entered column flow setpoint. The flow sensor
controls the EMPV current, causing the EMPV to correctly proportion the
column flow. The primary flow in excess of the required column flow volume
is directed to the waste.
Solvent degassing is not done directly in the pump. A 4-channel, low volume
micro vacuum degasser, available as a separate module, provides degassed
solvents to the pump channel inputs. Solvent degassing is required for best
flow stability and detector stability, especially at the low flow rates required to
run nano LC applications.
The flow range of the nano pump is between 0.1 µl/min and 1 µl/min.