Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
Quaternary Pump
User Manual

1290 Infinity Quaternary Pump User Manual
Notices
© Agilent Technologies, Inc. 2012-2018
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Manual Part Number
G4204-90002 Rev. B
Edition
10/2018
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
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notice until the indicated condi-
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
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understood and met.

1290 Infinity Quaternary Pump User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1290 Infinity Quaternary Pump (G4204A).
1Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your Agilent 1290 Infinity Quaternary Pump.
4 Using the Module
This chapter explains the operational parameters of the Agilent 1290 Infinity
Quaternary Pump.
5 How to Optimize the Performance of Your Module
This chapter gives hints on how to optimize the performance or use additional
devices.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 1290 Infinity Quaternary Pump User Manual
In This Guide...
8 Test Functions and Calibrations
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity
Quaternary Pump.
10 Parts and Materials
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the controller
software.
14 Appendix
This chapter provides addition information on safety, legal and web.

1290 Infinity Quaternary Pump User Manual 5
Contents
Contents
1Introduction 9
Features 10
Overview of the Quaternary Pump 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
System Overview 18
2 Site Requirements and Specifications 21
Site Requirements 22
Physical Specifications 25
Performance Specifications 26
3 Installing the Module 29
Unpacking the Module 30
Optimizing the Stack Configuration 31
Installation Information on Leak and Waste Handling 36
Removing the Transport Foam 40
Installing the Pump 41
Flow Connections to the Pump 43
Installation of Seal Wash Function 47
4 Using the Module 49
Magnets 51
Turn on/off 52
Status Indicators 54
Leak and Waste Handling 55
Best Practices 56
Setting up the Pump with the Instrument Control Interface 61
Flushing the Filter 73
Purging the Pump 75
Solvent Information 77
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260
Infinity II Flexible Pumps 84
Algae Growth in HPLC Systems 89

6 1290 Infinity Quaternary Pump User Manual
Contents
5 How to Optimize the Performance of Your Module 91
Operational Hints for the Multi Channel Gradient Valve (MCGV) 92
Delay Volume and Extra-Column Volume 93
How to Configure the Optimum Delay Volume 94
How to Achieve Higher Resolution 96
Using Solvent Calibration Tables 99
6 Troubleshooting and Diagnostics 101
Available Tests vs User Interfaces 102
Agilent Lab Advisor Software 103
7 Error Information 105
What Are Error Messages 107
General Error Messages 108
Pump Error Messages 116
8 Test Functions and Calibrations 133
Pump Leak Rate Test 134
System Pressure Test 139
9 Maintenance 141
Introduction to Maintenance 143
Warnings and Cautions 144
Overview of Maintenance 145
Cleaning the Module 146
Installing Fittings and Capillaries 147
Replacing the Pressure Sensor 148
Replacing the Inlet Weaver 151
Replacing the Inlet Valve 153
Replacing the Outlet Valve 155
Removing the Jet Weaver 159
Installing the Jet Weaver 161
Replacing the Seal Wash Pump 163
Replacing the Multi-Channel Gradient Valve (MCGV) 165
Releasing a Stuck Inlet Valve 169
Remove the Pump Head Assembly 172

1290 Infinity Quaternary Pump User Manual 7
Contents
Pump Head Maintenance (Tool Free) 176
Install the Pump Head Assembly 191
Replacing the Multi Purpose Valve 193
Replacing Parts of the Multi Purpose Valve 196
Replacing the Outlet Filter 198
Installing the Inline Filter 200
Removing the Inline Filter 202
Replacing Parts of the Inline Filter 204
Installing the Valve Rail Kit 207
Replacing Module Firmware 208
Preparing the Pump Module for Transport 209
10 Parts and Materials 215
Overview of Main Assemblies 216
Flow Connections 218
Seal Wash Function 219
Pump Heads 220
Multi Purpose Valve 224
Solvent Cabinet 226
Cover Parts 228
Leak Parts 229
Accessory Kit 230
HPLC System Tool Kit 231
11 Identifying Cables 233
Cable Overview 234
Analog cables 236
Remote Cables 238
BCD Cables 241
CAN/LAN Cable 243
RS-232 Cable Kit 244
Agilent 1200 Module to Printer 245

8 1290 Infinity Quaternary Pump User Manual
Contents
12 Hardware Information 247
Firmware Description 248
Electrical Connections 251
Interfaces 253
Setting the 8-bit Configuration Switch 260
Early Maintenance Feedback 264
Instrument Layout 265
13 LAN Configuration 267
What You Have To Do First 268
TCP/IP Parameter Configuration 269
Configuration Switch 270
Initialization Mode Selection 271
Dynamic Host Configuration Protocol (DHCP) 273
Link Configuration Selection 276
Manual Configuration 277
PC and User Interface Software Setup Setup 283
14 Appendix 285
General Safety Information 286
Waste Electrical and Electronic Equipment (WEE) Directive 292
Radio Interference 293
Sound Emission 294
Agilent Technologies on Internet 295

9
1290 Infinity Quaternary Pump User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Quaternary Pump 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
System Overview 18
Leak and Waste Handling 18
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 1290 Infinity Quaternary Pump User Manual
1 Introduction
Features
Features
The G4204A Quaternary Pump is designed for highest performance, GLP
compliance and easy maintenance. It includes the following features:
•Seal wash function for continued high lifetime of pump seals for buffer
applications.
•Optional Jet Weaver for optimum mixing performance with a minimum of
delay volume.
•Automatic purge function for ease of use and unattended preparation of the
system.
•Auto tuning of the delivery cycle for compensation of elasticity and solvent
volume change effects (compressibility, thermal expansion).
•Solvent Calibration Tables for optimum flow accuracy.
•Fast defill function for improved intake and delivery performance.
•Two pistons in series design for increased reliability.
•High resolution piston movement control for smooth and reliable motion.
For specifications, see “Performance Specifications” on page 26.

1290 Infinity Quaternary Pump User Manual 11
Introduction
1
Overview of the Quaternary Pump
Overview of the Quaternary Pump
The Agilent 1290 Infinity Quaternary Pump has a built-in 4-channel vacuum
degasser for best flow stability, especially at low flow rates and maximum
detector sensitivity. It uses a multi-channel gradient valve (MCGV) for
formation of quaternary gradients at low pressure. The low-pressure Inlet
Weaver based on patented Agilent microfluidic technology ensures highest
mixing performance and lowest mixing noise. The pump head offers a high
power range with a maximum pressure of 1200 bar and a maximum flow rate
of 5 mL/min. The Multi Purpose Valve can be used for automatic purging,
using an optional Jet Weaver high-performance mixer, automatic back-flushing
of the optional inline filter or for diagnostic.
The Agilent 1290 Infinity Quaternary Pump is suitable for a wide range of
columns and HPLC und UHPLC applications starting from typical
250 x 4.6 mm HPLC columns going down to high resolution 50 x 2.1 mm
UHPLC columns and can be used in a flow range between 0.05 – 5 mL/min.
The active seal wash function can be used with concentrated buffer solutions.

12 1290 Infinity Quaternary Pump User Manual
1 Introduction
Operating Principle
Operating Principle
The pump head comprises two pump chambers in series with independent
high-resolution motion control. A pressure sensor in the flow path monitors
the pressure. The pump control uses this signal for minimizing the pressure
ripple in order to achieve highest flow precision. A stable flow can be
delivered even in case of eventual small internal leaks, which can be
compensated automatically. A heat exchanger between two pump chambers
strongly reduces thermal effects due to solvent compression under very high
pressures.
As solvents are compressed by the pump head and expand further down the
flow path, for example in the column, the volumetric flow is changed
depending on the compressibility of the liquid. Agilent control software allows
specifying pure solvents, pre-mixed solvents and solvent gradients. Associated
Agilent solvent libraries are used by the pump control for enhanced flow
accuracy, which is required for cross-instrument or cross-system
reproducibility and method compatibility.
A high resolution encoder unit is attached to the pump drives, which divides a
single turn into 65000 steps. Each step corresponds to a volume of about
300 pL, which allows an extremely precise control.

1290 Infinity Quaternary Pump User Manual 13
Introduction
1
Operating Principle
Figure 1 The hydraulic path
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14 1290 Infinity Quaternary Pump User Manual
1 Introduction
Positions of the Multi Purpose Valve
Positions of the Multi Purpose Valve
The Multi Purpose Valve allows easy software controlled switching between
different modes of operation.
Normal Operating Mode Without Mixer
In normal operating mode, the flow comes from the pump head, passes the
pressure sensor and arrives at the central port of the Multi Purpose Valve. The
flow leaves the valve through port 4 to the system (autosampler etc.).
Figure 2 Valve position in normal operating mode without mixer
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1290 Infinity Quaternary Pump User Manual 15
Introduction
1
Positions of the Multi Purpose Valve
Purge Mode
In purge mode, the flow is diverted to the waste container.
Figure 3 Valve position in purge mode
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16 1290 Infinity Quaternary Pump User Manual
1 Introduction
Positions of the Multi Purpose Valve
Normal Operating Mode With Jet Weaver and Optional Inline Filter
In this mode, the flow passes an optional Jet Weaver and the optional inline
filter. This configuration is recommended for special applications which
require an increased mixing efficiency.
Figure 4 Valve position in normal operating mode with Jet Weaver
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1290 Infinity Quaternary Pump User Manual 17
Introduction
1
Positions of the Multi Purpose Valve
Filter Flush Mode
This mode is used for cleaning the inline filter by back-flushing it. The flow
goes to port 5, passes the inline filter in opposite direction and leaves to the
waste through port 7.
Figure 5 Valve position in filter flush mode
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CAUTION
Damage to the valve
➔Use the filter flush mode only if the optional inline filter is installed.

18 1290 Infinity Quaternary Pump User Manual
1 Introduction
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste handling. It
is important that all security concepts are understood and instructions are
carefully followed.

1290 Infinity Quaternary Pump User Manual 19
Introduction
1
System Overview
Figure 6 Leak and waste handling concept (overview - typical stack configuration as
an example)
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20 1290 Infinity Quaternary Pump User Manual
1 Introduction
System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent.
The maximum volume for an individual bottle stored in the solvent cabinet
should not exceed 2.5 L. For details, see the usage guideline for the Agilent
1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been
shipped with the solvent cabinet, electronic copies are available on the
Internet).
The leak pan (2) (individually designed in each module) guides solvents to the
front of the module. The concept covers also leakages on internal parts
(e.g. the detector’s flow cell). The leak sensor in the leak pan stops the running
system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module to
the next, as the solvent flows into the next module’s leak funnel (3, B) and the
connected corrugated waste tube (3, C). The corrugated waste tube guides the
solvent to the next lower positioned module’s leak tray and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to waste.
The condense drain outlet of the autosampler cooler (5) guides condensate to
waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.