Agilent Technologies InfinityLab LC Series User manual

1290 Infinity II High-Speed Pumps
Agilent InfinityLab LC Series
User Manual
1290 Infinity II High-Speed Pumps User Manual

1290 Infinity II High-Speed Pumps User Manual
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Document No: SD-29000230 Rev. E
EDITION 09/2020
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1290 Infinity II High-Speed Pumps User Manual 3
In This Guide…
This manual covers:
•Agilent 1290 Infinity II High-Speed Pump (G7120A)
•Agilent 1290 Infinity II Bio High-Speed Pump (G7132A)
1Introduction
This chapter gives an introduction to the pump and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3UsingthePump
This chapter explains the operational parameters of the Agilent 1290 Infinity II
High-Speed Pumps.
4PreparingthePump
This chapter provides information on how to set up the module for an analysis and
explains the basic settings.
5 Optimizing Performance
This chapter gives hints on how to optimize performance or use additional devices.
6Troubleshootingand Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

1290 Infinity II High-Speed Pumps User Manual 4
8 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity II High-Speed
Pumps.
9 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
10 Identifying Cables
This chapter provides information on cables used with the modules.
11 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
13 Appendix
This chapter provides addition information on safety, legal and web.

1290 Infinity II High-Speed Pumps User Manual 5
Contents
1Introduction9
Agilent 1290 Infinity II High-Speed Pump 10
Agilent 1290 Infinity II Bio High-Speed Pump 12
Pump Principle 14
Leak and Waste Handling 16
2 Site Requirements and Specifications 21
Site Requirements 22
Physical Specifications 25
Performance Specifications 27
3UsingthePump33
Magnets 34
Turn on/off 35
Status Indicators 37
Best Practices 38
Purging the Pump 45
Recommendations for Bio-Compatible and Bio-Inert Systems 47
Recommendations for Degassers 49
Solvent Information 50
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and
1260 Infinity II Flexible Pumps 58
Algae Growth in HPLC Systems 64
Setting up the Pump with the Instrument Control Interface 65
Agilent Local Control Modules 75
4 Preparing the Pump 76
Capillary Coding Guide 77
Installing Capillaries 80

1290 Infinity II High-Speed Pumps User Manual 6
5 Optimizing Performance 85
Delay Volume and Extra-Column Volume 86
How to Configure the Optimum Delay Volume 87
How to Achieve Higher Resolution 88
Using Solvent Calibration Tables 91
6 Troubleshooting and Diagnostics 96
User Interfaces 97
Agilent Lab Advisor Software 98
Pump Leak Rate Test 99
System Pressure Test 104
7 Error Information 106
What Are Error Messages 108
General Error Messages 109
Pump Error Messages 117
8 Maintenance 133
Introduction to Maintenance 135
Warnings and Cautions 138
Overview of Maintenance 140
Cleaning the Module 141
Install Fittings and Capillaries 142
Remove and Install Doors 143
Replace the Shutoff Valve Panel 145
Replace the Pressure Sensor 146
Replace the Solvent Selection Valve (SSV) 148
Change Configuration or Replace the Jet Weaver 150
Replace the Seal Wash Pump 153
Replace the Inlet Valve (G7120A) 154
Replace the Inlet Valve (G7132A) 156
Release a Stuck Inlet Valve 158
Remove the Pump Head Assembly (G7120A) 160
Remove the Pump Head Assembly (G7132A) 163

1290 Infinity II High-Speed Pumps User Manual 7
Pump Head Maintenance (Tool Free) 166
Install the Pump Head Assembly (G7120A) 191
Install the Pump Head Assembly (G7132A) 193
Replace the Outlet Valve (G7120A) 195
Replace the Outlet Valve (G7132A) 198
Replace the Purge Valve Head 200
Replace Parts of the Purge Valve Head 202
Replace Parts of the High Pressure Filter Assembly (G7120A) 203
Replace Parts of the High Pressure Filter Assembly (G7132A) 205
Replace the Module Firmware 207
Prepare the Pump Module for Transport 208
9 Parts and Materials for Maintenance 212
Overview of Maintenance Parts (G7120A) 213
Overview of Maintenance Parts (G7132A) 214
Flow Connections (G7120A) 216
Flow Connections (G7132A) 218
Pump Heads (G7120A) 220
Pump Heads (G7132A) 225
Purge Valve (G7120A) 229
Purge Valve (G7132A) 230
Cover Parts 231
Accessory Kit (G7120A) 232
Accessory Kit (G7132A) 233
HPLC System Tool Kit 234
10 Identifying Cables 235
Cable Overview 236
Analog Cables 238
Remote Cables 240
CAN/LAN Cables 244
Agilent Module to PC 245
USB 246

1290 Infinity II High-Speed Pumps User Manual 8
11 Hardware Information 247
Firmware Description 248
Electrical Connections 251
Interfaces 253
Setting the 8-bit Configuration Switch 258
Early Maintenance Feedback 261
Instrument Layout 262
12 LAN Configuration 263
What You Have to Do First 264
TCP/IP parameter configuration 265
Configuration Switch 266
Initialization mode selection 267
Dynamic Host Configuration Protocol (DHCP) 269
Link configuration selection 272
Manual Configuration 273
PC and User Interface Software Setup Setup 278
13 Appendix 280
General Safety Information 281
Waste Electrical and Electronic Equipment (WEEE) Directive 287
Radio Interference 288
Sound Emission Certification for Federal Republic of Germany 289
Agilent Technologies on Internet 290

1290 Infinity II High-Speed Pumps User Manual 9
1Introduction
Agilent 1290 Infinity II High-Speed Pump 10
Product Description 10
Features 11
Agilent 1290 Infinity II Bio High-Speed Pump 12
Product Description 12
Features 13
Pump Principle 14
Leak and Waste Handling 16
Leak Sensor 19
Waste Concept 20
This chapter gives an introduction to the pump and an instrument overview.

1290 Infinity II High-Speed Pumps User Manual 10
1Introduction
Agilent 1290 Infinity II High-Speed Pump
Agilent 1290 Infinity II High-Speed Pump
Product Description
The Agilent 1290 Infinity II High-Speed Pump can enhance your efficiency
through high speed and chromatographic performance.
A low-delay-volume mixer allows you to run fast gradients for narrow-bore
applications for high laboratory efficiency.
The new 1290 Infinity II LC power range has a high instrument efficiency,
allowing you to run any HPLC and UHPLC method.
The full ISET range enables you to transfer existing methods from different
instruments, including current Agilent systems as well as instruments from other
manufacturers.
Active damping, automatic purge valve, new ultralow dispersion kits or low
delay-volume capability, combine to achieve high instrument and analytical
efficiency.
Figure 1 Overview of the High-Speed Pump
Status indicator
Leak drain
Power switch
Pressure sensor
Pump head B
Pump head A
Seal wash pump
Jet weaver
SSV

1290 Infinity II High-Speed Pumps User Manual 11
1Introduction
Agilent 1290 Infinity II High-Speed Pump
Features
•High performance in terms of accuracy and precision for flow and
composition.
•High power range combining high pressure up to 1300 bar and high analytical
flow rates up to 5 mL/min for high chromatographic performance.
•The unique Intelligent System Emulation Technology (ISET) enables the
emulation of current Agilent 1100, 1200 and 1260 Infinity series instrument,
as well as Waters Alliance, Waters H-Class, Waters Acquity and Shimadzu
Prominence.
•Low delay volumes (to 10 µL) for running fast gradients on narrow-bore
columns make this pump a suitable front-end for LC/MS applications.
•Active damping with independently controllable high resolution pump drives
and firmware-embedded tuning algorithms significantly reduce ripples and
associated UV noise.
•The new Agilent Jet Weaver mixer, based on multi-layer microfluidic
technology, facilitates high efficiency mixing in combination with low delay
volume for high UV-detector sensitivity.
•Integrated high efficiency degasser with low internal volume is based on PTFE
AF technology and has a fast change-over of solvents for purging and priming
the pump.
•Built-in active seal-wash for increased uptime.

1290 Infinity II High-Speed Pumps User Manual 12
1Introduction
Agilent 1290 Infinity II Bio High-Speed Pump
Agilent 1290 Infinity II Bio High-Speed Pump
Product Description
The 1290 Infinity II Bio High-Speed Pump is a binary UHPLC pump consisting of
biocompatible material for use in biopharma (critical quality attributes) and other
applications utilizing high salt and extreme pH conditions. It uses high-pressure
blending from two solvents at pressures up to 1300 bar and flow rates up to
5mL/min.
The 1290 Infinity II Bio High-Speed Pump has an exceptionally low delay volume,
achieving high throughput and highest resolution for the most demanding
applications, giving you highest confidence in your results. It is the ideal front end
for LC/MS applications.
Status indicator
Leak drain
Power switch
Pressure sensor
Pump head B
Pump head A
Seal wash pump
SSV
Jet weaver

1290 Infinity II High-Speed Pumps User Manual 13
1Introduction
Agilent 1290 Infinity II Bio High-Speed Pump
Features
•The biocompatible pump is resistant to corrosion from high salt
concentrations and harsh cleaning procedures for increased instrument
uptime.
•High salt tolerance and wide pH range offers increased flexibility.
•Power range combines ultrahigh pressure up to 1300 bar and high analytical
flow rates up to 5 mL/min for maximum UHPLC performance.
•Bio Jet Weaver Mixer, based on multilayer microfluidic technology, combines
highest efficiency mixing with lowest delay volume for highest UV-detector
sensitivity.
•Multi-purpose valve enables automated software-embedded functionality
such as switching the optional mixer in and out, flushing back the inline filter
or automated purging for reduced manual interaction.
•Integrated high-efficiency degasser with low internal volume is based on
PTFE AF technology and provides fast change-over of solvents for purging
and priming the pump.
•Built-in active seal wash increases instrument uptime.
•Lowest delay volumes down to 10 µL allow you to run ultrafast gradients on
narrow-bore columns, making this pump the ideal front-end for LC/MS
applications.

1290 Infinity II High-Speed Pumps User Manual 14
1Introduction
Pump Principle
Pump Principle
Agilent 1290 Infinity II High-Speed Pumps feature a dual pump head design for
the generation of binary gradients.
The solvent selection valve allows the choice of two solvents per pump head.
However, this valve cannot be switched during a run.
Each pump head is equipped with two independently actuated pistons in series.
Delivery cycle:
1Piston two moves forward to deliver solvent into the flow path. The flow-rate
is thereby determined by the speed of the piston.
At the same time, piston one draws solvent from the solvent reservoir. The
two piston chambers are isolated by a check valve (outlet ball valve).
2Shortly before the end of the delivery stroke of piston two, piston one reverses
its direction. The check valve (passive inlet valve) at the inlet of piston
chamber one closes while the solvent in piston chamber one gets
compressed to system operating pressure.
3Piston two reverses while piston one delivers the set flow rate into the flow
path and re-fills piston chamber two.
4When piston two has reached the end of its intake stroke it reverses and the
delivery cycle starts again with step 1)
Reproducible solvent properties are maintained by an integrated two-channel
solvent degasser. It is located between the solvent selection valve and the pump
heads.
The pump automatically compensates for pressure and flow instabilities caused
by the complex relationship between solvent compressibility and system
pressure.
The only user interaction is selecting the appropriate solvent or solvent mixture
per channel from a drop-down list.

1290 Infinity II High-Speed Pumps User Manual 15
1Introduction
Pump Principle
Figure 2 The hydraulic path
Solvent
Jet Weaver
Valve
Degassing
unit
Pump head A Pump head B
SSV
High-Speed Pump
Seal wash pump Seal wash sensor
Pressure sensor
to Multisampler

1290 Infinity II High-Speed Pumps User Manual 16
1Introduction
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve

1290 Infinity II High-Speed Pumps User Manual 17
1Introduction
Leak and Waste Handling
Figure 3 Infinity II Leak Waste Concept (Flex Bench installation)

1290 Infinity II High-Speed Pumps User Manual 18
1Introduction
Leak and Waste Handling
Figure 4 Infinity II Single Stack Leak Waste Concept (bench installation)

1290 Infinity II High-Speed Pumps User Manual 19
1Introduction
Leak and Waste Handling
Figure 5 Infinity II Two Stack Leak Waste Concept (bench installation)
The waste tube connected to the leak plane outlet on each of the bottom
instruments guides the solvent to a suitable waste container.
Leak Sensor
CAUTION
Solvent incompatibility
The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible
with DMF.
Do not use DMF as mobile phase.
Check the leak sensor regularly for corrosion.

1290 Infinity II High-Speed Pumps User Manual 20
1Introduction
Leak and Waste Handling
Waste Concept
1Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with
4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use
your own waste solution, make sure that the tubes don't immerse in the liquid.