Biosan OD Plate User manual

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Contents
1. Safety Precautions................................................................................4
2. Introduction...........................................................................................5
3. Specifications .......................................................................................6
4. Complete Set........................................................................................6
5. Perform a Verification Test with OD Plate Verification Software............7
6. Interpretation of the Measurement Results............................................9
7. Troubleshooting..................................................................................10
8. Storage...............................................................................................13
9. Cleaning.............................................................................................14
10. Repair.................................................................................................14
11. Calibration ..........................................................................................15

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1. Safety Precautions
The following symbols m ean:
Caution: Make sure you have fully read and understood the
present Manual bef o r e using the instrument.
Please pay special attention to sections marked by
this sy m b o l .
GENERAL SAFETY
Use only as specified in the operating manual p r ov ided .
Do not use the unit if dropped or da m aged .
Store and transport the unit in a horizontal position (see package la-
bel).
Before using anycleaning or decontamination methods except those
recommended by t he manufacturer, check with the manufacturer that
the proposed method will not damage t he equip m en t.
Do not make any modifications to the design of the unit.
DURING OPERATION
Do not scratch the glass filters.
Do not use outside the laboratory r oom s.
Do not operate the unit if it is faulty or has been repaired in co rr e c tl y .
BIOLOGICAL SAFETY
 It is the user’s responsibility to carry out appropriate decontamination
if haza r do us material is spilt on or penetrates into the equip m en t.

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2. Introduction
OD Plate is a performance quality instrument plate reader photome-
ter MPP-96 HiPo. The purpose of this product is to verify the accuracy and
precision (trueness and reproducibility) measurement of photometer at 6 ab-
sorbance levels: 0.3, 0.6, 1.0,2.0, 3.0, 4.0 Abs (nominal). Thestandard range
is at following 17 Centre Wavelengths (CWL (± 1 nm)): 405, 414, 450, 480,
492, 515, 540, 550, 560, 568, 580, 594, 620, 632 , 650, 690, 700.
Optionally, the plate can be verified at any following Centre Wave-
lengths (CWL (± 1 nm)): 400, 405, 414, 420, 422, 430, 436, 440, 442, 445,
450, 455, 458, 460, 470, 473, 480, 486, 488, 492, 500, 505, 508, 510, 515,
520, 532, 540, 546, 550, 560, 568, 580, 589, 594, 600, 610, 620, 632, 636,
640, 647, 650, 656, 660, 671, 676, 680, 685, 690, 694, 700 nm.
The plate comes in a shockproof case and USB drive containing the
verification data and software.
Included is copy of the calibration certificate of reference materials
issued by accredi t ed b o dies.

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3. Specifications
3.1. Absorbance Levels ...........................0.3, 0.6, 1.0, 2.0, 3.0, 4.0 Abs.
3.2. Available calibration wavelength range.............................................
...............................................52 λin range 400 to 700 nm (± 1 nm)
3.3. Plate Dimensions .................................................128 x 86 x 12 mm
3.4. Plate Weight .......................................................................... 0.2 kg
3.5. Plate Design
4. Complete Set
- OD Plate
- USB Flash Drive with Software
- Manual
- Shockproof case

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5. Perform a Verification Test with
OD Plate Verification Software
5.1. For the verification, use the OD Plate (Catalogue number BS-
050108-AK)
5.2. Plug the flash drive provided with the plate in the com pu t er .
5.3. Open the OD Plate verification software.exe
5.4. Choose the verification plate from the list. The 4 digits number stick-
ered on the side of t he plate is the plate’s ID n um ber .
5.5. Choose the channels you need to verify. If you have additional chan-
nels, choose them. The wavelength of Channel 1/2/3/4 are written
body of the unit.
5.6. Set the number of measurements. Recommended is 20, minimum is
10.
5.7. Enter the name of the QC operator, Head of QC and QC company in
the empty fields.
5.8. Press Start.
5.9. When finished, press Results bu tt o n.
5.10. Test type should be Verif . Other test types are for manufacturers
use only.

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5.11. Press Calculate .
5.12. In the Protocol tab press Save to PDF in order to export the verifi-
cation certificate. Also available is the .csv export.
5.13. Print the report, sign it and leave with the customer.
5.14. Save the report and send it to the manufacturer: [e-mail]
5.15. Here is a sample of a verification report if test is passed:

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6. Interpretation of the
Measurement Results
6.1. Accuracy.
Accuracy is determined for selected wavelengths at six absorbance
levels, each of which covers eight well positions in a row. Each of the
20 measurements of the OD plate is compared to the accuracy crite-
ria as follows:
(Test value –Reference absorbance value) × 100 < Accuracy criteria.
- The accuracy test will be passed only if all test values meets the
specifications
Accuracy
Criteria
0 –2.3 OD
≤ (0.5 % ± 0.010 OD) typical
2.3 –3 OD
≤ (1 % ± 0.010 OD) typical
3 –4.3 OD
≤ (2 % ± 0.010 OD) typical
4.3 –5 OD
≤ (15 % ± 0.010 OD) typical
6.2. P r ecision
Precision here is the coefficient of variation (CV%), which is deter-
mined for any row after reading the OD Plate 20 times. The CV% is
calculated as f o l lows:
𝐂 =
𝑺
𝑴×𝟏𝟎𝟎%
Where Cis CV%, Sis Standard deviation between and Mis M ean.
The precision test will be passed only if the average precision at each
absorbance level and at each channel meets the specifica t ion s.
Precision
Criteria
0 –2.3 OD
≤ 0.5 %
2.3 –3 OD
≤ 1.0 %
3 –5 OD
≤ 2.0 %

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7. Troubleshooting
7.1. The accuracy or precision test have failed.
In case of failed tests, the reason may be in faulty or unclean instru-
ment or OD Plate. Generally, if one or more of the precision tests
have FAILED, the reason lies in the ins t r um en t or OD plate, if it was
assembled. If one or more of the accuracy tests have FAILED, the
r eas o n may lie in the instrument or in the OD Plate. Here is the list of
to do things:
Check that the OD Plate has been placed correctly onto the plate
carriage. The A1 markings on the instruments should coincide and
the OD plate should lie flat.
Check that the glasses are c lean.
If the fault does not disappear, contact the technical service r ep r e-
sen t a t i v e .
7.2. The device cannot connect to the com pu t er .
Install the driver by opening “Drivers/Driver.exe” on the provided USB
flash drive and restarting your de v ices/s o ft wa r e/com pu t er after.
7.3. The program cannot close, says that the experiment is still go-
ing, but I stopped it.
Try pressing the Play button on the toolbar (to start the experiment),
and click on the S t o pbutton, wait 5 seconds and then try to close it.
If this does not work, open Task Manager (C t r l + Shift + Esc) and
close all processes “OD plate verification software.exe”
7.4. Device does not respond to the p r og ra m .
Try turning the device on or off. If necessary, try unplugging it and
reconnecting.

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7.5. Drivers cannot be installed
Try giving administrator rights to the user who installs the p r og ra m.
7.5.1. If the previous step did not help, try the f o l lo w in g:
Go to Control Panel - Device M anager
Expand the Other devices line, right-click the USB Serial Port:
Click on Properties, Select Driver tab, click on the Update Dr i v er :

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Select Search automatically for updated driver software. You
should have internet co nne c t ion on.
After installing the driver, the following message should appea r :
7.5.2. If you do not have an internet connection, please use computer with
internet to dow nload latest drivers from
http://www.ftdichip.com/Drivers/VCP.htm under “Available as s et up
executable”. Then, transfer and install the driver on the computer to
which the units are co nne c t ed .
7.5.3. If it does not help, please try a solution described her e:
h tt ps://supp o r t.micros o ft.co m/en-us/h e l p/2654149/er r o r -u sb-device-no t-
re cog nize d-w h en-you-t r y-to-access-a-u sb-externa l-h a r d-dr i v e
7.6. I have connected the device to USB 2.0 SS (super speed) termi-
nals and my computer shu ts down constantly.
There is some sort of an error with FTDI driver.
Please avoid connecting to USB 2.0 SS, connect the devices to the
standard USB 2.0. D a t a for connecting to USB 3.0 terminals is not
yet available.

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7.7. When I was disassembling/assembling the plate, I found some
breaks / scratches on the glass. What should I do?
If the breaks/scratches are only localized outside the plate’s aper-
tures, it is ok, although c he c k regularly if it did not expand to the ap-
erture. In thecases the breaks/scratches arein theapertures,contact
the technical support for further ac t ion.
7.8. The glass ID number stickers can be seen in the apertures. Is
this O K?
The working aperture of the HiPo MPP-96 is only 2 mm in diameter,
so if you have received the plate with some small part of the stickers
located inside the bigger aperture (6.8 mm in diameter), it is O K.
7.9. My problem is not described here .
While doing the verification process, a problem that is not described
here may arise. C o n t ac t the technical support in such c as es.
8. Storage
Always store the OD Plate in its own case when not in use to keep
the glass surface free o f dust. In general, avoid storage sites with excessive
dust, dirt, moisture or large t empera t ure fluctuations.

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9. Cleaning
9.1. If dust particles can be visually detected on the surface of glasses,
remove the dust with clean compressed air.
9.2. If any spillages can be seen, clean the glasses with a microfibre c lo t h
moistened with ethanol (70-96%), rinse the glasses with distilled wa-
ter and dry them w e ll .
9.3. To disassemble the ins t r um en t:
- Rotate the OD plate upside down (the side with A1 marking faces
surface).
- Loosen the black countersunk screws from the sides.
- Unscrew the facing to you bottom side.
- We suggest taking a picture on a smartphone for the ease of r ea s-
semb l y .
- Carefully remove the glass for c leaning .
9.4. Handle the glasses with care. Never use an abrasive cleaning mate-
rial that may scratch t he glasses.
9.5. Do not touch the glasses with your hands. Use gloves.When assem-
bling be sure that g l asses (especially on the ends of the plate) lies
flat.
10. Repair
We do not deliver or accept any separate parts for recalibration or
repair. Faulty g l asses can only be replaced by provider officials. For repairs,
contact your technical ser v ice r ep r esen t a t i v e .

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11. Calibration
The calibration is traceable through the VSL – NEDERLANDS ME-
TR O L O GISCH INSTITUUT and METROSERT Ltd. The traceability chain
is shown belo w :
The absorbances of the reference glasses are calibrated at the inter-
vals of five years at the V S L at several wavelengths between 400 nm –700
nm. The reference glass is then used to calibrate the Reference
I ns t r u-
m en t. The glasses from the OD plate is then calibrated by measuring the
absorbances of the g l asses with the reference ins t r um en t.
The uncertainty of the calibration is calculated according to the pub-
lication EA -4/02 (Expression of the Uncertainty of Measurement in C a li b ra-
t ion). The main uncertainty factors are the uncertainty of the calibration of
the reference glass and the repeatability of the m easuremen ts.
Reference glass VSL
and Metrosert
Reference Instrument
OD Plate

Edition 1.02 –March of 2020