Biosan RCP-24 User manual

2 Edition 1.06
If you have any feedback on our products or services, we would like to hear from you.
Please send all feedback to:
Manufacturer:
SIA Biosan
Ratsupites 7 k-2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
https://biosan.lv
Marketing: [email protected]
Service: [email protected]

Edition 1.06 3
Contents
1. About this edition of the manual...............................................................................3
2. Safety precautions ...................................................................................................4
3. General information..................................................................................................6
4. Getting started..........................................................................................................7
5. Operation .................................................................................................................8
6. Recommended homogenization parameters..........................................................10
7. Specifications.........................................................................................................10
8. Ordering information ..............................................................................................11
9. Care and maintenance...........................................................................................12
10. Troubleshooting errors...........................................................................................12
11. Warranty.................................................................................................................14
12. EU Declaration of conformity..................................................................................15
1. About this edition of the instructions
1.1 The current edition of the user instructions applies to the following models:
Model and name
Version
RCP-24, reciprocal laboratory homogenizer
V.1AW
1.2 Edition 1.06 –July of 2022

4 Edition 1.06
2. Safety precautions
Caution!
Make sure you have fully read and understood the present manual before
using the equipment. Please pay special attention to sections marked by
this symbol.
Caution!
This device has been developed and tested in accordance with CISPR 11
Class A (EN 61326-1/EN 55011). The device may cause radio interfer-
ence in domestic environments and is not intended for use in residential
areas. The device cannot ensure acceptable protection of radio reception
in residential areas and domestic environments. If necessary, take suita-
ble actions to eliminate the interferences.
2.1 General safety
•The protection provided by the equipment may be impaired if the equipment is used
with accessories not provided or recommended by the manufacturer, or used in a
manner not specified by the manufacturer.
•Save the unit from shocks and falling.
•Store and transport the unit as described in section 10. Storage and transportation.
•Use only original parts and accessories, provided by manufacturer for this product.
•Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
•Do not make modifications in design of the unit.
2.2 Electrical safety
•Connect only to the mains with voltage corresponding to that on the serial number
label.
•Do not plug the unit into an ungrounded powersocket, and donot use an ungrounded
extension lead.
•Ensure that the power plug is easily accessible during use.
•Disconnect the unit from the mains before moving.
•If liquid penetrates into the unit, disconnect it from the mains and have it checked by
a repair and maintenance technician.
•Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in the 7. Specifications section.

Edition 1.06 5
2.3 During operation
•Do not start the operation without the rotor tightened firmly and less than 2 microtubes
loaded and symmetrically positioned in the inner ring of the rotor.
•Do not open the lid of the unit during operations.
•Do not operate the unit in environments with aggressive or explosive chemical mix-
tures. Please contact manufacturer for possible operation of the unit in specific at-
mospheres.
•Do not operate the unit if it is faulty or has been installed incorrectly.
•Do not use outside laboratory rooms.
•Do not lean on the unit during operation.
•Do not operate the unit if it is faulty or was installed incorrectly.
•Always fix the rotor securely. Stop the operation immediately with the Stop key if any
unusual noise occurs during acceleration, which can be due to improper rotor fixation.
2.4 Biological safety
•The user is responsible to carry out appropriate decontamination if hazardous mate-
rial spills on or penetrates into the equipment.

6 Edition 1.06
3. General information
Reciprocal Homogenizer RCP-24, a bench-top mechanical device designed for mix-
ing, grinding, homogenizing and emulsifying biological objects in microtubes by vigorously
mixing by reciprocal motion with various beads for the purpose of sample preparation for
subsequent academic, pharmaceutical, biotechnological or biomedical studies.
Homogenizer facilitates the formation of a supernatant containing nucleic acids and
proteins suitable for subsequent purification, extraction or analysis. The device is optimized
for extracting proteins, DNA, RNA or tRNA from various tissue sources, but it can also be
used for other applications. RCP-24 performs efficient homogenization of mammalian tissue,
plant tissue or other biomaterials.

Edition 1.06 7
4. Getting started
4.1 Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does notcover in-transit damage. Warrantycovers only the units
transported in the original package.
Caution!
Due to the high weight of the unit, its unpacking and installing must be
carried out by two persons.
4.2 Complete set. Package contents:
- RCP-24, Reciprocal Laboratory Homogenizer.................................................1 pce.
- Power cable ....................................................................................................1 pce.
- Rotor stand.......................................................................................................1 pce.
- Emergency lid opening tool (located at the rear of the unit) ............................1 pce.
- User instructions, certificate ...........................................................................1 copy
1. Rotor stand 2. Rotor 3. Rotor lid
Figure 1.
4.3 Setup.
- Place the unit on a stable, horizontal and even working surface, and clear 20 cm
around the unit on all sides for ventilation and safety of operation.
- Connect the power cable to the socket on the rear side of the unit, position it with an
easy access to the power switch and plug.

8 Edition 1.06
5. Operation
5.1 Startup.
5.1.1 Attach the power cable to the mains. Switch the Power switch on the rear of the unit
into position I(on).
5.1.2 The display lights up with the following indications:
•Set time (min:sec, fig. 2/1), parameter selection indicator <or >(fig. 2/2) and set
speed (fig. 2/6) in the top row.
•Indication STOP (fig. 2/3) and current speed (fig. 2/7) in the bottom row.
Figure 2. Control panel
5.2 Inserting the samples.
5.2.1 Add the samples to the microtubes with beads, add lysis buffer solution (if required)
according to your method or Biosan’s recommended homogenization parameters
(see section 6).
5.2.2 Open the protective lid of the unit by pressing the ▲ open (fig. 2/8). Display shows
the OPEN indication.
5.2.3 Unscrew the rotor lid (fig. 1/3), remove it, then load the samples into the rotor (fig.
1/2).
Caution!
Insert the first 2 tubes opposite to each other into the rotor sockets, main-
taining balance. If more tubes are loaded, further symmetrical positioning
in the rotor sockets is not required.
5.2.4 Tightly screw back the rotor lid.
5.2.5 Close the protective lid of the unit by pushing firmly with two hands on both sides of
the lid. If the lid locking is successful, display shows the STOP indication.
5.3 Setting parameters.
5.3.1 Using the ◄ and ► SELECT keys (fig. 2/5), choose between time and homogeniza-
tion speed. The <or >indicator (fig. 2/2) marks the selected parameter.
5.3.2 Using the +and –keys (fig. 2/4), set the time (fig. 2/1) and speed (fig. 2/6) of the
operation. Hold the key pressed to change the values faster.

Edition 1.06 9
5.4 Running the operation.
5.4.1 Press the ►/■ RUN/STOP key (fig. 2/9) to start homogenization. Display shows the
RUN indication (fig. 2/4) and current speed (fig. 2/7). Timer starts the countdown
when set RPM is achieved. Speed can be changed during the operation.
Caution!
If the unit stops the operation and the display shows the IMBALANCE
indication, then, either the rotor is not fixed correctly, or the tubes are not
loaded correctly, or the rotor is empty. Open the lid by pressing the ▲
open key and resolve the issue accordingly.
5.4.2 After finishing the program, the unit makes a sound signal, accompanied with a blink-
ing indication STOP on the display. Press the ►/■ RUN/STOP key to stop the signal
and return to standby mode.
5.4.3 The operation can be stopped at any moment by pressing the ►/■ RUN/STOP key.
Timer resets to the starting values.
5.5 Power interruption. In case of mains/power failure, the unit is not equipped with
automatic restart function, which requires the user to reinitiate the platform motion
manually.
5.6 Ending the operation.
5.6.1 Press the ▲ open key, unscrew the rotor lid and remove it.
5.6.2 Remove the samples.
5.6.3 After finishing the operation, move the Power switch to position О(off). Disconnect
the unit from the mains. The rotor and rotor lid can remain in the unit.
Note.
Lid can be unlocked by pressing the ▲ open key only when the unit is
powered. Switch on the unit as described in 5.1 or use the emergency
opening method (5.7).
5.7 Unit lid emergency opening.
5.7.1 Move the Power switch to position О(off). Disconnect the unit from the mains. Wait
for the rotor to stop.
5.7.2 Insert the included emergency tool from the holder located on the back of the unit
into the emergency opening slot near the lid until a click is heard.

10 Edition 1.06
6. Recommended homogenization parameters
6.1 The unit is capable of lysing various samples which can bedivided into 3 main groups:
•Soft tissues (plant, animal, human).
•Hard and dry tissues (bones, teeth, nails, seeds, rice, etc.).
•Microorganisms (microbes, fungi, spores, etc.).
6.2 Displayed in the table below are recommended parameters and beads for specific
biomaterial homogenization
Sample name
Bead type (material, size, density)
Setting,
(RPM)
Microbes (bacteria)
Glass, 0.1 mm, 2.5 g/cm3
2000
Microbes (yeast)
Glass, 0.5 mm, 2.5 g/cm3
Animal soft tissues (muscle, kidney, heart, spleen, etc.)
Ceramic, 2.8 mm, 6 g/cm3
2000
Leaves, Stems, Roots
Ceramic, 2.8 mm, 6 g/cm3
2000
Hard and dry tissues (bones, teeth, seeds, nails)
Stainless Steel, 2.8 mm, 7.9g/cc
2000
Unengorged ticks
Ceramic, 1.4 mm, 6 g/cm3
2000
Engorged ticks (fed)
Ceramic, 2.8 mm, 6 g/cm3
2000
6.3 Common rules to follow when designing the experiment –
•As material density increases, the kinetic energy of the bead increases, thus increas-
ing lysis efficiency.
•Higher density beads usually have a higher cost per experiment and not all samples
require the highest kinetic energy for lysis.

Edition 1.06 11
7. Specifications
The unit is designed for operation in cold rooms and closed laboratory rooms at am-
bient temperature from +4°C to +40°C, altitude up to 2000 m., in a non-condensing atmos-
phere, maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to
50% relative humidity at 40°C, transient overvoltages up to the II category and pollution de-
gree 2.
Biosan is committed to a continuous programme of improvement and reserves the
right to alter design and specifications of the equipment without additional notice.
Test tube rotor capacity............................................................................................up to 24
Test tubes.......................................................................................................................2 ml
Speed control range..................................................500–2000 RPM (increment 100 RPM)
Digital time setting.....................................................................1–15 min (increment 1 min)
Oscillation amplitude .................................................................................... 44 mm, vertical
Display...................................................................................................... LCD, 2 x 16 signs
Overall dimensions (W×D×H)...................................................................285×400×440 mm
Input voltage and frequency.................................................................230 V~ ±10%, 50 Hz
Power consumption.................................................................................................... 215 W
Operating current ......................................................................................................... 1.3 А
Weight, accurate within ±10% ...................................................................................19.1 kg
8. Ordering information
8.1 Models and versions available:
Model
Catalogue number
RCP-24, Reciprocal Homogenizer
BS-010701

12 Edition 1.06
9. Care and maintenance
9.1 Service.
9.1.1 If the unit is disabled (e.g., no rotor movement, no reaction to key presses, etc.) or
requires maintenance, consultthe troubleshooting table below. If the problem persists
or not described, disconnect the unit from the mains and contact Biosan or your local
Biosan representative.
9.1.2 All maintenance and repair operations (except listed below) must be performed only
by qualified and specially trained personnel.
9.1.3 Operating integrity check. If the unit follows the procedure described in section Op-
eration, then no additional checks are required.
9.1.4 Troubleshooting errors
Problem
Cause
Solution
Display not working
No mains/power connection or
failure, power cable not fully con-
nected
Check the mains/power connec-
tion.
Check the fuses.
Check the power cable
Lid cannot be open
The unit is still in operation
Wait for the unit to stop
No mains/power connection or
failure
Check the mains/power connec-
tion.
Check the fuses.
Use emergency tool to open the
lid
Unit shakes when in operation
There is no rotor inside the unit
or the first two tubes are not
loaded symmetrically
Stop the centrifuge and load the
first two tubes symmetrically, re-
start the unit.
Display error - Overheated
The internal temperature of the
unit is critical and the unit must
cool down
Allow the unit to cool down (at
least 15 min. recommended), re-
peat the run.
Display error - Temperature error
The internal temperature sensor
is not responding
Replace sensor
Display error - Imbalance
There is no rotor inside the unit
or the first two tubes are not
loaded symmetrically
Stop the centrifuge and load the
first two tubes symmetrically, re-
start the unit.
Microtube leakage
Too high temperature or pressure
inside the microtubes
Lower experiment time
9.2 Cleaning and disinfection.
9.2.1 Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse
remaining washing solution with distilled water. Wipe dry the excess water with clean,
soft cloth or sponge.
9.2.2 To disinfect the plastic and metal parts, use 75% ethanol or DNA/RNA removing so-
lution (e.g., Biosan PDS-250). After disinfecting, wipe the surfaces dry.
9.2.3 Rotor is autoclavable, at 121°С, for 15 min, the unit itself is not autoclavable.

Edition 1.06 13
9.3 Fuse replacement. Disconnect the unit and the power cable from the mains, then
remove the power cable from the socket on the rear panel of the unit. Open the fuse
holder (fig. 4/A). Check the fuse and replace if necessary. Fuse parameters - M 4 A
(type M- time lag: Medium), rating 250 V.
Figure 4. Fuse holder
9.4 Disposal. Disposal of the appliance requires special precautions and must be carried
out at an appropriate disposal site, separate from normal household waste. To pre-
vent pollution of the environment, all waste resulting from the disposal of the product
must be collected and disposed of in the country of use, in accordance with the ap-
plicable requirements for the handling of electronic waste.

14 Edition 1.06
10. Storage and transportation
10.1 Store and transport the unit in a horizontal position (see package label) at ambient
temperatures between -20°C and +60°C and maximum relative humidity of 80%.
10.2 After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
11. Warranty
11.1 The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and transporta-
tion instructions.
11.2 The warranted service life of the unit from the date of its delivery to the Customer is
24 months. For extended warranty, see 9.5.
11.3 Warranty covers only the units transported in the original package.
11.4 If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit Technical support page on our website at link below.
11.5 Extended warranty. For RCP-24, the Basic Plus class model, extended warranty is a
paid service. Contact your local Biosan representative or our service department
through the Technical support section on our website at the link below.
11.6 Description of the classes of our products is available in the Product class descrip-
tion section on our website at the link below.
Technical support
Product class description
biosan.lv/en/support
biosan.lv/classes-en
11.7 The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
Serial number
Date of sale
RCP-24,
Reciprocal Homogenizer
11.8 Production date. Production date is placed in the serial number, on the label of the
unit. Serial number consists of14 digits styled XXXXXXYYMMZZZZ, where XXXXXX
is model code, YY and MM –year and month of production, ZZZZ –unit number.

Edition 1.06 15
12. EU Declaration of conformity
12.1 Reciprocal laboratory homogenizer RCP-24 is in conformity with the following rele-
vant Union legislations:
LVD 2014/35/EU
LVS EN 61010-1:2011 Safety requirements for electrical equipment for
measurement, control, and laboratory use. General requirements.
LVS EN 61010-2-051:2015 Particular requirements for laboratory equip-
ment for mixing and stirring.
EMC 2014/30/EU
LVS EN 61326-1:2013 Electrical equipment for measurement, control and
laboratory use. EMC requirements. General requirements.
RoHS3 2015/863/EU
Directive on the restriction of the use of certain hazardous substances in
electrical and electronic equipment.
WEEE 2012/19/EU
Directive on waste electrical and electronic equipment.
12.2 Declaration of Conformity is available for download on the page for the relevant model
on our website by link below:
RCP-24

Biosan SIA
Ratsupites 7 k-2, Riga, LV-1067, Latvia
Phone: [phone] Fax: [phone]
https://biosan.lv
Edition 1.06 –July of 2022