Biosan DEN-1B User manual

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Contents
1. Safety Precautions .......................................................................................4
2. General Information......................................................................................6
3. Getting started..............................................................................................7
4. Operation....................................................................................................10
5. Specifications .............................................................................................12
6. Maintenance...............................................................................................13
7. Warranty. Reclamation information ............................................................13
8. Declaration of Conformity...........................................................................14

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1. Safety Precautions
The following symbol means:
Caution! Make sure you have fully read and understood the present
Manual before using the equipment. Please pay special at-
tention to sections marked by this symbol.
GENERAL SAFETY
Use only as specified in the Operating Manual provided.
Save the unit from shocks and falling.
After transportation or storage and before connecting it to the electric circuit,
keep the unit under room temperature for 2-3hrs.
Before using any cleaning or decontamination methods except those rec-
ommended by the manufacturer, check with the manufacturer that the pro-
posed method will not damage the equipment.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to an external power supply with voltage corresponding to that
on the serial number label.
Use only the external power supply provided with this product.
Ensure that the power switch and external power supply are easily accessi-
ble during use.
Disconnect the unit from the external power supply before moving.
If liquid penetrates into the unit, disconnect it from the external power supply
and have it checked by a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
Batteries
Use alkaline (preferred) or rechargeable AA type batteries.
ATTENTION! DANGER! Risk of explosion and burns:
The batteries must be inserted correctly with respect to polarity by following
the diagram in the battery case.
If one battery is reversed (two + poles or two - poles in contact with each
other), a chemical reaction is produced in minutes that releases explosive
gasses and extremely corrosive liquid.
In case of doubt, turn off the unit immediately and check the polarity.
Protect your eyes in case a leakage is detected. Cover the battery case with
a rag before opening it to avoid contact with any discharge.
In case of contact with liquid from the batteries, rinse the affected area
immediately with clear water and get immediate medical attention.

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Do not mix brands of batteries.
Do not mix new and used batteries.
Remove the batteries from the unit for prolonged storage.
Do not recharge alkaline batteries.
Do not short-circuit the batteries as this can cause burns.
Do not attempt to open or dismantle batteries.
Do not put used batteries in a fire.
Keep batteries out of reach of children.
Keep water out of the battery case.
Follow the disposal instructions and properly dispose of the used battery.
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemi-
cal mixtures. Please contact manufacturer for possible operation of the unit
in specific atmospheres.
Do not operate the unit if it is faulty or was installed incorrectly.
Do not use outside laboratory rooms.
Use the Select and Install buttons only for calibration of the unit according
to p. 3.4. Do not press the buttons in other cases, as this can cause calibra-
tion reset and may require recalibration.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if
hazardous material is spilt on or penetrates into the equipment.

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2. General Information
DEN-1B Densitometer is designed for solution turbidity measurement in the
range of 0.0 - 6.0 McFarland units (0 cells/ml - 18x106cells/ml). DEN-1B is capable
of measuring solution turbidity in a wider range (6.0 - 15.0 McFarland units)
however, it is necessary to remember that in this case the standard deviation values
increase.
DEN-1B Densitometer is used for 1) determining concentration of cells (bac-
terial, yeast cells) in the fermentation process, 2) detection of susceptibility of
microorganisms against antibiotics, 3) identification of microorganisms with various
test systems, 4) measuring optical density at fixed wavelength and 5) quantitative
evaluation of concentration of dyed solutions that absorb green light.
The operation principle is based on optical density measurement with digital
result representation in McFarland units.
The unit is calibrated at the factory and saves calibration data when being
switched off. However, in can be calibrated by 2-8 points in 0.0 - 6.0 McFarland unit
range if necessary. Both commercial standards (e.g. produced by Prolab, Lachema,
BioMerieux, etc.) and the cell suspensions prepared in the laboratory can be used
for calibration.
Table 1. Interpretation of McFarland Standard results into corresponding numeric
values of bacterial suspension concentration and their optical density at 550 nm.
McFarland
Standard
Composition
Interpretation
Concentration BaSO4
Bacterial
concentration*
Theoretical optical
density at 550 nm**
0.5
2.40x10-5 mol/l
150x106cells/ml
0.125
1
4.80x10-5 mol/l
300x106cells/ml
0.25
2
9.60x10-5 mol/l
600x106cells/ml
0.50
3
1.44x10-4 mol/l
900x106cells/ml
0.75
4
1.92x10-4 mol/l
1200x106cells/ml
1.00
5
2.40x10-4 mol/l
1500x106cells/ml
1.25
* Bacterial concentration depends on microorganism size. The numbers represent an
average value valid for bacteria. For yeasts, which are larger in size, these numbers
should be divided by about 30.
** Values correspond to optical densities of bacterial suspensions. The BaSO4solutions
optical density values differ, because the particle size and form differ from those of
bacteria and light is diffracted differently.

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3. Getting started
3.1. Unpacking
Remove packing materials carefully and retain them for future shipment or
storage of the unit.
Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage.
Warranty covers only the units transported in the original package.
3.2. Complete set. Package contents:
Standard set
- DEN-1B Densitometer suspension turbidity detector.......................... 1 piece
- AA type battery..................................................................................3 pieces
- external power supply ........................................................................ 1 piece
- Operating Manual, Certificate.............................................................. 1 copy
- A-16 adapter for tubes........................................................................ 1 piece
Optional accessories
- CKG16 calibration kit for glass tubes 16 mm in diameter..............on request
- CKG1802 calibration kit for glass tubes 18 mm in diameter..........on request
3.3. Battery set up:
- Insert a flat sharp pin into the small socket according to the fig.1/1 on the
underside and open the battery compartment.
- Insert the batteries inside as shown on the installation scheme in the battery
compartment.
- Place the unit on the horizontal even working surface;
- Alternatively, connect the external power supply to the socket (fig.2/2) on the
rear side of the unit.
Fig.1 Bottom panel

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3.4. Calibration.
The device is pre-calibrated at the factory for operation with the glass tubes
16 mm in external diameter (see the label on the bottom side of the unit) at
temperature range from +15°C to +25°C and saves calibration data when
being switched off.
Note! To work with other type tubes (e.g. with different outer
diameter, bottom shape or different material such as trans-
parent plastic tubes), it is necessary to perform calibration in
the specified tubes, using adapters, if necessary.
Perform calibration in the following sequence from the lower calibration value
to the higher values: 0.00, 0.50, 1.00, 2.00, 3.00, 4.00, 5.00 and 6.00. Use at
least 2 points for calibration.
3.4.1. If the external power supply is used, connect it to electric circuit.
3.4.2. Switch ON the unit using the Power switch (Fig.2/1) on the rear panel
(position I).
Attention! Make sure that the tube socket is empty!
Note! Use a thin pin of maximum diameter 2 mm for pressing the
Select and Install buttons.
3.4.3. Press the Select button (fig.2/3) on the backside of the unit. A flashing ”-.--”
indication will be shown on the display, showing that the unit is ready to save
calibrations value of the first calibration point - the empty socket.
3.4.4. Press the Install button (fig.2/4), the empty socket calibration value will be
saved in the unit memory and the next calibration value is displayed (flashing
figure ”0.00”).
Note! It is required to calibrate the 0.00 value and recommended to
calibrate as many points as possible to obtain precise re-
sults. The minimum requirement is to calibrate 2 points clos-
est to the working range limits (e.g. 0.00 and 6.00 for opera-
tion in 0.00 - 6.00 McF unit range).
Fig.2 Rear panel

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Note! If the standard for 0.00 value is not available, fill the tube (of
the kind that is used for operations and standards) with dis-
tilled water; use the tube as the 0.00 value standard.
3.4.5. Insert the tube with the standard solution, corresponding to the calibration
point value, into the socket (fig.3/1) of Densitometer.
Note! Shake the tube with the standard solution, if necessary. It is
recommended to use a vortex, e.g. Personal Vortex V-1 plus,
for shaking.
3.4.6. Press the Install button. This calibration curve value will be saved in the
memory and the next calibration value will be displayed.
Note! If pressing the Install button during the calibration process
does not cause switching to the next standard value, it
means that the currently inserted in the densitometer socket
standard has lower turbidity value than the previous stand-
ard. The reason is that the inserted standard solution turbidi-
ty does not correspond to the required value (the standard is
to be shaken or replaced).
3.4.7. Repeat steps 3.4.5.-3.4.6. until the calibration is complete (1-7 times, i.e. as
many times as many points the chosen calibration curve has).
3.4.8. If a standard is not available, press the Select button for the next calibration
value to be displayed.
3.4.9. After installing the last standard value 6.00, or skipping it (by pressing the
Select button), the will exit the calibration mode automatically. The unit is
ready for operation.
3.5. Reset to factory calibration. To reset the calibration of the unit to factory
settings, ensure that you are in the working mode and the socket of the unit
is empty. Press and hold Install key for 5 seconds. The unit displays a dot ” .
“, then changes it to “0.00”. The unit is now reset.
3.6. Switch off the unit using the Power switch (position O). If an external power
supply is used, disconnect the external power supply from electric circuit.

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4. Operation
Recommendations during operation
Remove the tube with the solution being measured before switching the unit
on or off.
It is recommended to keep the unit switched on for 15 min before starting the
operation in order to stabilize it in the working mode.
If flat-bottomed tubes are used, the solution level should be higher than 7
mm from a tube bottom; if round-bottomed tubes are used, the level should
be higher than 12 mm from a tube bottom.
The display goes off if there is no tube in the socket during one minute.
Press the On key (fig.3/3) to activate the unit.
Check if the A-16 adapter is in the socket (Fig. 3/1). The device is calibrated
for operation with the glass tubes 16 mm in external diameter. Refer to
p. 3.4. when using different tubes.
Fig.3 General view
4.1. If an external power supply is used, connect it to electric circuit.
4.2. Switch ON the unit using the Power switch (Fig.2/1) on the rear panel
(position I).
4.3. The following indication may be shown on the display (fig.3/2):
“0.00” the unit is calibrated and ready for operation (if no tube
inserted);
“LO BAT”Low Battery: when operating on batteries, replace the
batteries following the instructions of the Safety Pre-
cautions section concerning batteries.

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4.4. Shake the tube with the solution. It is recommended to use a vortex for
shaking, e.g. V-1 plus personal vortex. Insert the tube into the socket of den-
sitometer (fig.3/1). The McFarland value for the solution will be shown on the
display (fig.3/2).
4.5. Glass and transparent plastic tubes (16 or 18 mm in external diameter) can
be used for work with densitometer. An A-16 adapter must be inserted in the
socket when working with tubes which have external diameter 16 mm.
Note! The unit must be calibrated each time a tube type, e.g. with
different outer diameter, bottom shape or different material
transparent plastic tubes), is changed.
4.6. After finishing the operation switch OFF the unit using the Power switch
(position O). If the external power supply is used, disconnect it from electric
circuit.

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5. Specifications
The unit is designed for operation in cold rooms, incubators and closed la-
boratory rooms at ambient temperature from +4°C to +40°C in a non-condensing
atmosphere and maximum relative humidity 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
5.1. Light source.............................................................................................LED
5.2. Wavelength............................................................................ λ = 565 ±15 nm
5.3. McFarland unit range ......................................................................0.0 - 15.0
5.4. Display resolution............................................................................0.01 McF
5.5. Accuracy, of the full scale.......................................................................+ 3%
5.6. Measurement time ....................................................................................1 s
5.7. Sample volume.........................................................................2 ml minimum
5.8. Recommended external diameter of tube
..............................................................16 mm (using A-16 adapter) or 18 mm
5.9. Display ....................................................................................................LCD
5.10. Dimensions ..........................................................................165x115x75 mm
5.11. Input current/power consumption .....................................12 V, 7 mA / 0.1 W
5.12. External power supply ...........input AC 100-240 V 50/60 Hz, output DС12 V
5.13. Battery...........................................................................3 x batteries AA type
5.14. Weight*................................................................................................. 0.7 kg
Replacement
parts
Description
Catalogue number
A-16
Adapter for tubes 16 mm in external diameter
BS-050102-AK
Optional
accessories
Description
Catalogue number
CKG16
Calibration kit for glass tubes 16 mm in
diameter. Latex particles.
BS-050102-BK
CKG1802
Calibration kit for glass tubes 18 mm in
diameter. Barium sulphate BaSO4.
BS-050102-GK
Biosan is committed to a continuous program of improvement and reserves
the right to alter design and specifications of the equipment without additional
notice.
* Accurate within ±10%.

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6. Maintenance
6.1. If the unit requires maintenance, disconnect the unit from the electric circuit
and contact Biosan or your local Biosan representative.
6.2. All maintenance and repair operations must be performed only by qualified
and specially trained personnel.
6.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning
of laboratory equipment can be used for cleaning and decontamination of
the unit.
7. Warranty. Reclamation information
7.1. The Manufacturer guarantees the compliance of the unit with the require-
ments of Specifications, provided the Customer follows the operation, stor-
age and transportation instructions.
7.2. The warranted service life of the unit from the date of its delivery to the
Customer is 24 months (excluding the consumables, i.e. batteries and cali-
bration kits). Contact your local distributor to check availability of extended
warranty.
7.3. Warranty covers only the units transported in the original package.
7.4. If any manufacturing defects are discovered by the Customer, an unsatisfac-
tory equipment claim shall be compiled, certified and sent to the local distrib-
utor address. Please visit www.biosan.lv, Technical support section to obtain
the claim form.
7.5. The following information will be required in the event that warranty or post-
warranty service comes necessary. Complete the table below and retain for
your records.
Model
Suspension turbidity detector Densitometer DEN-1B
Serial number
Date of sale

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8. Declaration of Conformity

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Biosan SIA
Ratsupites 7, build. 2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
http://www.biosan.lv
Edition 2.02 –January 2016