MRC UT-200A User manual

Operation Manual
UT-200A
Microplate Reader
PLEASE READ THIS MANUAL CAREFULLYBEFORE OPERATION
Hagavish st. Israel 58817 Tel: [phone], Fax: [phone] [email protected]
MRC.1.22

Version modification Record:
Version No. Date Modification Description
V1.0 2019.12.16 Initial Release
V2.0 2020.5.11 Revised performance parameters and
add accessories included

I
Foreword
Thank you for purchasing our Microplate Reader. This user manual describes how
the instrument works and the operation guide, please read carefully before
operation and keep for future reference.
Opening check
Please check the instruments as well as all accessories with packing list when
you first open it. If you find any wrong or missing, please contact distributor or
manufacturer.

II
Safety Warning and Guidelines
1. Important information for safe use
Any improper operation without reading manual is forbidden, otherwise
there will be risks in cause accidental injury or electrical shock.
Do read manual carefully and operate safely according to this guidelines.
This instrument intended to use in Scientific Research Only!
2. Safety Tips
The operation, maintenance and repair of the Instrument should comply with the
basic guidelines and the remarked warning below. If you don’t comply with them, it
will have effect on the scheduled using life of the Instrument and the protection
provided.
Indoor use only.
Warning: Biological contamination!! All samples for test, quality
control, calibration are regarded as infectious, and any part contact
with samples will also need to be treated as infectious. Please wear
gloves when operate this device.
Before using the device, read the Manual carefully. These units are
designed for use in laboratory environments. The device must be
used by skilled personnel with the appropriate training.
Warning: Avoid injury. Keep your body or any part of body away 15cm
(or more) from the instrument when running.
Users should have a clear main idea on how to use this instrument before
operate, do read this user manual carefully.

III
The operator should not open or repair the Instrument by himself,
which will result in losing the qualification of repair guarantee or occur
accident. If there is some wrong with the Instrument, please contact
manufacturer for repair.
Before power on, guarantee the voltage used should be accordant to
the voltage needed, and the rated load of electrical outlet should
not
lower than the demand.
If the electric line is damaged, you should replace it with the same
type. You should assure there’s nothing on the electric line and you
should not put the electric line in the ambulatory place.
Hold the socket when you pull out the plug, and don’t pull the electric
line only.
The Instrument should be put in the place of low temperature, less
dust, no water and no sun or strong lamp. What’s more, the place
should be good ventilation, no corrosively gas or strong disturbing
magnetic field, far away from central heating, camp stove and
other
hot resource.
Power off when you finish your work. Pull off the connector plug when
there’s long time no use of the Instrument and cover it with a cloth or
plastic paper to prevent from dust.
Pull the connector plug from the socket at once in the following cases,
and contact the vendor:

There is some liquid flowing into the Instrument;

Drenched or fire burned.

Abnormal operation: such as abnormal sound or smell.

Instrument dropping or outer shell damaged.

Malfunction

Contents
Foreword.................................................................................................................................................... I
Opening check.......................................................................................................................................... I
Safety Warning and Guidelines............................................................................................................ II
1. Important information for safe use........................................................................................... II
2. Safety Tips................................................................................................................................... II
Chapter 1 Brief Introduction................................................................................................................ 1
Chapter 2 Features............................................................................................................................... 2
Chapter 3 Instrument Structure.......................................................................................................... 3
Chapter 4 Installation............................................................................................................................4
1. Opening check............................................................................................................................. 4
2. Installation.....................................................................................................................................4
3. Installation steps.......................................................................................................................... 4
Chapter 5 Operation Guide................................................................................................................. 6
1. Instrument self-check..................................................................................................................6
2. System settings............................................................................................................................7
3. Protocol Management.................................................................................................................8
4. Read a Microplate..................................................................................................................... 10
5. Result processing...................................................................................................................... 16
6. Report exporting........................................................................................................................ 23
7. Power off..................................................................................................................................... 23
Chapter 6 Maintenance, storage, transportation........................................................................... 24
1. Maintenance............................................................................................................................... 24
2. Storage and transportation...................................................................................................... 24
Chapter 7 Trouble shooting............................................................................................................... 25
Chapter 8 Accessories.......................................................................................................................... 26

Microplate Reader Operation Manual Chapter 1 Brief Introduction
─1─
Chapter 1 Brief Introduction
This automatic microplate reader UT-200A is professional instrument for
hetELISA , measuring concentration, absorbance, positive or negative of
-Linked antibody and antigen in the sample by testing the color of the Enzyme
Immunosorbent Assay (ELISA). This reader is widely used in clinical test,
biology agriculture, food and environment research, especially benefit from
ELISA kits increasingly wide utilization.
Highlights:
1) With 10-inch touch screen.
2) Operating system allows acquisition, editing and saving of data.
3) It can be used alone, and also connect with PC by ReaderIt-II software
for plenty of data analysis.
4) 96-well visual layout allows easy setting of blank, sample,
positive/negative, quality control and multi-value comparison.
5) With dual optical system, as well as reference optical channel which
guarantee stable detection data.
6) End point method, kinetics and spectral scanning are available, as well
as plates with or without lids.
7) Xenon lamp with long lifetime which can reach to 109times.
8) With incubation function, the average temperature deviation between
wells≤0.5°C.
9) Self-checking optical path, top reading and mechanical motion.
10) With shaking function, time and speed are adjustable.
11) It supports USB data export, fast and easy to operate.
12) System multi-user hierarchical operation, easy for data management.

Microplate Reader Operation Manual Chapter 2 Features
─2─
Chapter 2 Features
Working conditions：
Ambient temperature: 10C~40C
The relative humidity: 30%~80%(No condensation)
Power: AC100-240V 50-60Hz 2A
Parameters:
Model
Parameter UT-200A
Light source Xenon flash lamp >109flashes
Wavelength 200～1000nm
Bandwidth ≤ 2.5nm
Wavelength accuracy 2nm
Wavelength
repeatability 0.2nm
Read-out range 0.0-4.0 OD
Linearity@450nm R2 ≥ 0.999 , [0.0 - 3.0]
Accuracy@450nm ±(1.0% + 0.003A) , (0 - 2.0]
±2.0% , (2.0 - 2.5]
Precision@450nm CV < 0.5% (Precision mode); CV < 1.0% (Fast mode)
Stability@450nm < 0.005A , (0.0 - 2.0]
< 2% , (2.0 - 2.5]
Measurement speed <8 seconds at Fast mode (96-well plate)
<28 seconds at Precision mode (96-well plate)
Plate shaking Linear
Incubation range RT+ 4°C to 45°C
Temp. uniformity ± 0.5°C @ 37°C
Connections
1 B-type of USB port for PC
1 Ethernet port
2 A-type of USB ports for devices
Power requirements DC24V 6.67A 160W
Dimension (W×D×H) 300×500×260mm
Weight (kg) 15.5kg

Microplate Reader Operation Manual Chapter 3 Instrument Structure
─3─
Chapter 3 Instrument Structure
Structure
Rear cover
Touch screen
Roof cover
External Button
Plate holder
Front door
Extension module
USB Port
LAN Port
Switch
Power Socket
B-type
USB Port
Air outlet

Microplate Reader Operation Manual Chapter 4 Installation
─4─
Chapter 4 Installation
Opening check .1
Each UT-200A is thoroughly tested before shipping, but please check again when you
:receive the instrument and contact your local distributor or manufacturer if
• The outer package is damaged
• The outer package has any obvious moisture stains
• The outer package has marks of impact
• The outer package has signs of being opened
After opening, please check the instrument and box contents.
Confirm that all ordered accessories have been included.
Check the instrument’s appearance for any damage.
2. Installation
Working condition: locate instrument on a flat dry and clean work table, keep the front
side with enough space for plate holder in and out, also keeping 15cm space for back, left
and right side to enable put or connect wires.
Working environment:
a. Clean air free from corrosion steam or smoke.
b. Temperature should be within the range of +10°C ~ +40°C.
c. Relative humidity should be within the range of 30% ~ 80% to avoid condensation.
Note: KEEP INSTRUMENT AWAY FROM DESTRUCTIVE GAS OR LIQUID!
3. Installation steps
1Place the instrument on a stable and level surface.
Note: Please DO NOT loose any screw or parts without permission, or it will
cause instrument damage and make it out of warranty.
2Connect the instrument to an appropriate power outlet using the provided power
cord.
Fig. 1

Microplate Reader Operation Manual Chapter 4 Installation
─5─
Note: Attention to the interface of the power adapter, please connect it to the
power according to the direction of the above picture.
3Switch “I/O” button to “I” to turn on the instrument, the front panel will cycle
through a start-up and self-test screen.
Warning: Don’t connect instrument to power socket without ground wire.

Microplate Reader Operation Manual Chapter 5 Operation Guide
─6─
Chapter 5 Operation Guide
1. Instrument self-check
This chapter introduces default protocol operation,beginning with self-check after power on.
Refer to the picture below:
Fig 2
User login interface will appear after self-checking, see Fig 3.
Fig 3

Microplate Reader Operation Manual Chapter 5 Operation Guide
─7─
Table 1
User type Creation
Method Default password Permission Export
Admin Can not be
deleted“123456”
For all files of
Admin, User and
Guest All can be
exportedUser Created by
Admin
Default is “123456” or
set when creating Only for their own
files
GuestCan not be
deleted No password
Note: Please keep the Admin password, or contact the manufacturer or your
distributor when forgotten the password of Admin.
Home interface as below Fig 4.
Fig 4
The “Admin” button on the lower left is for logout to login interface.
2. System settings
Users can can make system settings according to their needs, details see Fig 5.

Microplate Reader Operation Manual Chapter 5 Operation Guide
─8─
Fig 5
Note: 1. The instrument need to be restarted after date and time settings finished.
2. The function of maintenance is only for manufacturer use, does not open for
users.
3. Click “Home” button on top left corner for the main interface.
3. Protocol Management
Click “Absorbance” to Fig 6 interface. This interface mainly composed by six parts:
the navigation bar at the top, the sidebar, the main display area, the optional bar,
switching bar/type select area and the status bar at the lower right area.
Fig 6
The navigation bar
The optional bar
Switching bar
The Sidebar
The main display area

Microplate Reader Operation Manual Chapter 5 Operation Guide
─9─
Protocol name can be modified by clicking it directly.
The default name of a new protocol is the system time,
and it can be modified by manual. A hint will pop-up when
a modified protocol not saved.
Save is for the current protocol saving which can be
found in protocol list.
A shortcut for exporting raw data to U disk in format of
csv.
Including sorting, delete, import, export, rename and
save as etc. Protocol list will be appear if clicking
“Protocols” button, see Fig 7, click blank area will close
the protocol list interface.
Unfold state Protocol edit state
Fig 7

Microplate Reader Operation Manual Chapter 5 Operation Guide
─10 ─
When the protocol list is in unfold state, users can do below operations:
Search: Enter keyword to carry out searching automatically.
Sorting: protocols can be sorted according to “Name”, “Date” and “State”. “demo1”,
“demo2” and “demo3” are always line on the first three position.
Import: importing protocols from U disk to instrument.
Rename: for protocol name changing.
Save as: save as a new protocol.
Edit: “Edit” button is locate on the top right corner, see Fig 7 unfold state.
Account: Protocols of other accounts can be checked, but this function is only
available for Admin account.
When protocol list in edit state, below operations are available:
Sorting: protocols can be sorted according to “Name”, “Date” and “State”.
Checkbox: “” can be chosen for more than one for batch operation.
Delete: delete selected protocols.
Export: for exporting selected protocols to U-disk.
Cancel: Return to the unfold state of protocol list.
4. Read a Microplate
After protocol created, the next step is parameter settings according to experiment
requirements.
4.1 Plate layout setting
The interface will move to layout interface automatically after finishing create a new
protocol.
Fig 8

Microplate Reader Operation Manual Chapter 5 Operation Guide
─
11 ─
Meanwhile, the switching bar will turn out to be type select which includes 6 sample
types, as well as clear option. Choose the right sample type, the optional bar will change
accordingly, then click corresponding wells of the main display area to finish the settings.
Note: the whole plate can be set if clicking the blank area on top left corner of the
main display area.
With white color on the interface, it is as blank control
during running measurement. All blanks are duplicate
wells in a same group.
Standard sample well which is used for creating standard
curve, it is with green color. The optional bar will changed
after clicking “Standard”, see Fig 9.
Replicates: switch it on when setting the same
standard for multiple wells.
Concentrations: After setting the concentration and
unit of the first well, the protocol will finish subsequent
standard samples automatically according to the set
operator and step size, also users can modify by
manual by clicking corresponding wells.
Eg.: If the set concentration is 125ng/μL, the
operator is “×” and the step size is “2”, the
concentration of the first well should be 125ng/μL, the
second well 250ng/μL and the third 500ng/μL… etc.
Sample groups: one sample group can have one
standard curve.
For quality control during tests, it is with light green. It
includes replicates and sample group, settings are the
same as wells of standard samples.
With blue color, users can set several wells as unknown
well, besides replicates and sample group, it has another
option “Factor” for the dilution times of the solution, so as
to get the concentration of the solution directly.
1: X means a solution was diluted by X times.
With orange color, users can set several wells as positive,
as well as sample groups.
With purple, also several wells can be set as negative,
and sample groups as well.

Microplate Reader Operation Manual Chapter 5 Operation Guide
─12 ─
For clearing sample well settings.
Fig 9
Note: Elisa plate layout can be carried out before or after testing, for example,
users can set all wells as “unknown”, do absorbance test, then layout of plate. The
test data and layout are separated, users can modify or analyze the layout of
historical data in real time.
Remark: the absorbance value of the test can not be changed!
4.2 Parameter setting
After finishing the Elisa plate layout settings, click “Run” on the side bar to Fig 10.
Fig 10

Microplate Reader Operation Manual Chapter 5 Operation Guide
─13 ─
Meanwhile the switching bar will turn out to be controller which mainly includes below
functions:
Incubator: this function can make the plate detection chamber up to specified
temperature, the temperature displays is the real time temperature.
Wavelength: shows wavelength parameters of the current protocol.
Shake: this function can be on or off
Progress bar: displays the running status of the current protocol.
Plate in/out: control the plate holder in and out, also there is Physical button on the
front of the enclosure.
Start/Stop: also Physical button on the front of the enclosure.
Shake: this button is independent from the shaking function in settings, and
specially used for plate defoaming.
Meanwhile, the optional bar turns out to be step settings which separated into three
settings according to different specific measurement method: Endpoint, Kinetic and
Spectrum, for more details see below table 2.

Microplate Reader Operation Manual Chapter 5 Operation Guide
─14 ─
Table 2
Endpoint
Kinetic
Spectrum
┗Selected Parameters ┗Selected Parameters ┗Selected Parameters
┗Wavelength
┗Mode
┗Fast
┗Precision
┗Wavelength
┗λ1 (405)
┗λ2 (450)
┗λ3 (492)
┗λ4 (630)
┗Wavelength
┗Mode
┗Fast
┗Precision
┗Wavelength
┗λ1 (405)
┗λ2 (450)
┗λ3 (492)
┗λ4 (630)
┗Wavelength
┗Mode
┗Fast
┗Precision
┗Wavelength
┗Start Wavelength
┗End Wavelength
┗Step
┗Kinetic
┗Total Time
┗Total Time
┗Kinetic region
┗No. of readings
┗Number
┗Kinetic region
┗Shake
┗Speed
┗Low
┗Medium
┗High
┗Type
┗Continuous
┗Pulsed
┗Time
┗Shake
┗Speed
┗Low
┗Medium
┗High
┗Type
┗Continuous
┗Pulsed
┗Time
┗Shake
┗Speed
┗Low
┗Medium
┗High
┗Type
┗Continuous
┗Pulsed
┗Time
┗Wait Time at start ┗Wait Time at start ┗Wait Time at start
┗Area Selection ┗Area Selection ┗Area Selection
About wavelength option, Endpoint and Kinetic configured with four
wavelengths(defaults are 405nm, 450nm, 492nm and 630nm), users can click modify
wavelength by manual, but the value must be within the range 200nm~1000nm. Spectral
analysis can accept wavelength of any band, but also should be within 200nm~1000nm.
In addition, there is a button named “Log” on top right corner of the main display area,
the button is only available after the current protocol been executed.
The “Log” is mainly used for recording the finishing time of each step of the protocol.
Note: Steps can not be edited if a protocol been executed, users can save it as a new
one and then edit.