Knauer Smartline 2800 User manual

PDA Detektor 2800/PDA Detector 2800
Benutzerhandbuch/User Manual
V7606

1
Content
Using this Manual................................................................................. 3
Conventions in this manual............................................................. 3
SOP’s in this manual....................................................................... 3
General Description of PDA Detector 2800 ......................................... 4
Optical Path of the PDA Detector 2800 .......................................... 5
Preparing the PDA Detector 2800 for Operation ................................. 5
Unpacking ....................................................................................... 5
Front and Rear View of the PDA Detector 2800............................. 6
Power supply, ON/OFF................................................................... 8
The detector’s position in a KNAUER Smartline system ................ 8
Operating the PDA Detector 2800 ....................................................... 9
Function of Foil Key and LEDs........................................................ 9
Installation of the Smartline PDA Detector 2800............................. 9
Installation of the Flow Cell........................................................... 12
Capillary Connection to an HPLC System .................................... 13
Switching on the Detector ............................................................. 15
Software Control of the PDA Detector 2800 ...................................... 16
Operating the Detector with ChromGate®..................................... 16
Detector Setup ......................................................................... 16
Settings and Initial Tests.......................................................... 18
Operating the Detector with EuroChrom®..................................... 21
Detector Setup ......................................................................... 21
Settings and Initial Tests.......................................................... 23
Connecting other Instruments to the PDA Detector 2800.................. 25
Using the Remote Control Socket................................................. 25
Simple Maintenance........................................................................... 27
Control of the Lamp functionality .................................................. 27
Checking Wavelength Accuracy ................................................... 27
Changing the Lamp....................................................................... 28
Cleaning the flow cell .................................................................... 29
Adjusting the Path Length of Preparative Flow Cells........................... 32
Problems and possible Causes..................................................... 32
Spare Parts and Accessories............................................................. 33
Flow cells for the Smartline PDA Detector 2800........................... 33
Spare Parts and Accessories, Order Numbers............................. 33
Specifications ..................................................................................... 34
Declaration of conformity.................................................................... 69
Warranty Statement ........................................................................... 70
Index(english)..................................................................................... 71

2
Inhalt
Hinweise zum Gebrauch des Handbuchs ..........................................35
Konventionen in diesem Handbuch...............................................35
SOP’s in diesem Handbuch ..........................................................35
Allgemeine Beschreibung des PDA Detector 2800............................36
Optischer Weg des the PDA Detector 2800..................................37
Installation des PDA Detector 2800 ...................................................37
Auspacken.....................................................................................37
Front- und Rückansicht des of the PDA Detector 2800................38
Netzanschluss, ON/OFF ...............................................................40
Die Position des Detektors im KNAUER Smartline System..........41
Betrieb des PDA Detector 2800 .........................................................42
Funktion der Folientasten und LEDs.............................................42
Installation des Smartline PDA Detector 2800..............................43
Installation der Messzelle..............................................................45
Kapillaranschluss an ein HPLC-System........................................47
Einschalten des Detektors.............................................................48
Softwaresteuerung des Smartline PDA Detector 2800......................49
Betrieb des Detektors mit ChromGate®.........................................49
Detektor Setup .........................................................................49
Einstellungen und erste Tests..................................................51
Betrieb des Detektors mit EuroChrom ®........................................54
Detector Setup .........................................................................54
Einstellungen und erste Tests..................................................56
Verbindung anderer Geräte mit dem PDA Detector 2800..................58
Verwendung der Fernsteuerungsleiste .........................................58
Einfache Wartung...............................................................................60
Kontrolle der Lampenfunktion .......................................................60
Überprüfung der Wellenlängenrichtigkeit......................................60
Lampenwechsel.............................................................................61
Messzellenreinigung......................................................................63
Analytische Messzellen............................................................63
Analytische 3 mm Messzellen..................................................64
Präparative Messzellen............................................................65
Einstellung der Weglänge präparativer Messzellen......................65
Fehler und ihre mögliche Behebung .............................................66
Ersatzteile und Zubehör .....................................................................67
Messzellen für den Smartline PDA Detector 2800........................67
Ersatzteile und Zubehör, Bestellnummern....................................67
Technische Daten...............................................................................68
Gewährleistungsbedingungen............................................................69
Konformitätserklärung ........................................................................70
Index(deutsch)....................................................................................72

Using this Manual 3
Using this Manual
This manual is valid for any combination of the Smartline PDA Detector
2800 with analytical flow cells order number A4130, A4131, A4132,
preparative flow cells order number A4133, A4134, A4135, A4136 and
A4137.
Conventions in this manual
Important tips are marked by the marginal hand symbol.
Special warnings are indicated by the marginal warning sign and
printed in bold letters.
The marginal lamp symbol indicates helpful advice.
SOP’s in this manual
The Standard Operating Procedures (SOP) provided with this manual
offer a convenient way of structuring complex tasks in the operation of
your Smartline PDA Detector 2800. They include step-by-step
instructions leading the user through all routine tasks during operation.
They can be used for documentation purposes and be copied, applied
signed, and filed in order to document the performance of the instrument.
Please operate the instrument and all accessories according to
instructions and SOP’s in this manual. This ensures safe operation,
accurate results and prolongs the longevity of your equipment.
SOP 1 Displaying / Setting the S2800’s IP address ...............................................10
SOP 2 How to find out a PC’s IP address...............................................................11
SOP 3 How to change a PC’s IP address...............................................................11
SOP 4 Communication check..................................................................................11
SOP 5 Installation of the Flow Cell..........................................................................12
SOP 6 Capillary connections...................................................................................13
SOP 7 Assembling WAGO plugs ............................................................................26
SOP 8 Wavelength Accuracy Check.......................................................................27
SOP 9 Changing the Deuterium Lamp....................................................................28
SOP 10 Purging the Flow Cell...................................................................................29
SOP 11 Disassembling and Cleaning of an Analytical 10 mm Flow Cell.................30
SOP 12 Disassembling and Cleaning of an Analytical 3 mm Flow Cell...................31
SOP 13 Disassembling and Cleaning of a Preparative Flow Cell............................31
SOP 14 Changing the Path Length of Preparative Flow Cells.................................32

4 General Description of PDA Detector 2800
General Description of PDA Detector 2800
Fig. 1 Smartline PDA Detector 2800
The Smartline PDA Detector 2800 is based on advanced fiber optics
technology. It combines excellent technical specifications with
outstanding operability.
No other instrument of this kind provides such a wide wavelength range
(when equipped with deuterium and tungsten-halogen lamp) matched
with high light source intensity up to the NIR range. The detector has a
very high resolution at 0.8 nm per pixel and operates with an impressive
wavelength accuracy of < 0.5 nm and a wavelength reproducibility
(precision) of < 0.1 nm. The Smartline PDA Detector 2800 delivers the
best results in terms of sensitivity, noise and drift.
Due to its unique design the Smartline PDA 2800 has no delicate moving
parts to break down, operates without a reference channel or special “see
through” lamps, and does not require a time consuming cold-start
calibration. The instrument is completely controlled by the HPLC
software. Its Ethernet interface provides for easy and secure connection
to a common TCP/IP network or directly to a PC, enabling the highest
possible data rates.
The Smartline PDA Detector 2800 can simultaneously acquire up to four
chromatograms and perform spectra scans at a maximum rate of 10 per
second (the scan range can be selected from 190 – 1,020 nm). In
conjunction with the chromatography software this diode array detector
offers features like 2-D and 3-D chromatograms, spectra libraries, peak
purity tests, and more. This detector provides two adjustable software-
controlled analog outputs for the support of preparative applications.
The variety of available flow cells and the option to easily extend the fiber
optics for positioning the cell outside the housing, make this detector
highly flexible.

Preparing the PDA Detector 2800 for Operation 5
Optical Path of the PDA Detector 2800
Fig. 2 Optical path of the PDA Detector 2800 with two lamps (diagram)
The main parts of the KNAUER Smartline PDA Detector 2800 are shown
in Fig. 2. The light from both lamps (in the two lamps version) is focused
by separate optics, passes separate shutters and goes through two fiber
optical cables into the light mixer which provides perfectly balanced
mixing of ultraviolet, visible and near infrared light. All light then goes
through the flow cell and into the polychromator where it is split into a
spectrum of 190 to 1020 nm (one lamp version: 190 to 610 nm). The
whole spectrum is detected by a 1024 diodes (one lamp version: 512
diodes) diode array ( 0.8 nm per diode)..
The KNAUER Smartline PDA Detector 2800 UV has no halogen lamp,
just one shutter and no light mixer.
Preparing the PDA Detector 2800 for Operation
Unpacking
All KNAUER instruments are always packed carefully and safely for
transportation. After unpacking, please check the device and accessories
thoroughly for any damage that may have occurred during transport. If
necessary, put forward any claim for damages to the carrier.
Use the list “Standard delivery” and check if the PDA Detector 2800
delivery is complete. Please contact our service department if you are
missing something or if you need support.
Standard Delivery Smartline PDA Detector 2800
1. Smartline PDA Detector 2800 with
fiber optical cables, without flow cell
2. Operation manual
3. Power supply cable
4. Ethernet patch cable, 3 m, RJ-45
(blue cable)
5. Cross linked Ethernet patch cable,
3 m, RJ-45 (yellow/grey cable)
6. Three WAGO connectors (2-pronged)
and mounting device
7. Two flat cables (10 pins)
8. SMA/SMA Connector
9. two screws for flow cell installation

6 Preparing the PDA Detector 2800 for Operation
Front and Rear View of the PDA Detector 2800
Front Door of the Smartline PDA Detector 2800
4
5
6
1
2
3
Fig. 3 Front view of the PDA Detector 2800
The Smartline PDA Detector 2800 is a fully software controlled
instrument and has only the standby key and five LEDs on the front
Table 1 Functional elements on the front
1
Power LED
2
Busy LED
3
Error LED
4
UV lamp LED
5
VIS lamp LED
6
Standby key and LED
The Front Plate of the Smartline PDA Detector 2800
(behind the door)
1
2
Fig. 4 Front view with open door
Table 2 Functional elements on the front plate
1
SMA connectors for fiber optical cables
2
Flow cell holder

Preparing the PDA Detector 2800 for Operation 7
10
I
2
1
0
3
Fig. 5 analytical flow cell
Table 3 analytical flow cell
1
Inlet of the cell
2
Outlet of the cell
3
Path length indicator (10 mm)
Rear Panel of the Smartline PDA Detector 2800
1
2
3
4
5
6
7
8
Fig. 6 Rear view of the PDA Detector 2800
All available electrical connectors are located on the instrument’s rear
panel.
Table 4 Functional elements on the rear panel
1
Main switch
2
Power connector (integrated fuses)
3
Voltage selector
4
Remote control connectors
5
Communication board LEDs
6
RS-232 connector (only for setup)
7
LAN connector
8
LAN connection LEDs

8 Preparing the PDA Detector 2800 for Operation
Power supply, ON/OFF
The PDA Detector 2800 can be operated at 115 or 230 V AC (50-60 Hz).
The setting is done with the voltage selector on the instrument’s rear
panel. The standard setting is 230 V. The instrument can be switched off
from the main switch (on the back of the instrument) or the standby key.
Before using the instrument please check whether the setting of the
voltage selector fits with your local voltage.
Please note that the instrument is not completely switched off when
in standby-mode. Only with the main switch the instrument can be
completely switched off.
The detector’s position in a KNAUER Smartline system
Due to the general temperature sensitivity of detectors, the Smartline
PDA Detector 2800 should always be the first (lowest) instrument in a
Smartline system. The optionally available capillary kits for easy
installation will only fit if this rule is followed.
Fehler! Verweisquelle konnte nicht gefunden werden. shows a typical
Smartline system (low pressure gradient).
Fig. 7 Smartline system (low pressure gradient)
Smartline Manager 5000
Smartline Pump 1000
Smartline PDA Detector 2800

Operating the PDA Detector 2800 9
In the case that a Smartline RI Detector should also be arranged in the
tower, the RI detector should be installed below the PDA detector due to
it’s higher sensitivity to temperature.
Operating the PDA Detector 2800
Function of Foil Key and LEDs
The keypadFehler! Verweisquelle konnte nicht gefunden werden.
consists of a standby key with LED and five separate LEDs.
Standby key
Pressing the standby key for more than two seconds switches the
instrument off (only the standby electronics will still be working). In
standby mode the red LED integrated into the standby key is on. To
switch the instrument on again the standby key has to be pressed for
more than one second. Then the instrument powers up again and the red
LED is off.
Power LED
The Power LED lights when the instrument is switched on (main power
switch or standby key if switched off with standby key).
Busy LED
The Busy LED begins to light at low intensity when the connection
between detector and PC is established (e.g. when a method is started ).
The BUSY LED lights at high intensity when data is tranfered by the
detector.
Error LED
The Error LED lights when the instrument is switched on (main power
switch or standby key if switched off with standby key) and is switched of
as soon as a connection between detector and PC is established (e.g.
when a method is started). The Error LED lights up again when an
internal error occurred.
UV LED
The UV LED begins to light about 30 seconds after the connection
between detector and PC is established (e.g. when a method is started).
VIS LED
If the detector is equipped with a halogen lamp the VIS LED begins to
light when the connection between detector and PC is established (e.g.
when a method is started).
Installation of the Smartline PDA Detector 2800
PC Specifications
•Pentium III or higher
I
I

10 Operating the PDA Detector 2800
•256 MB RAM or more
•20 GB hard disk or more
•Ethernet interface
•Chromatography Software: KNAUER ChromGate®system software
V 3.1 or higher. KNAUER EuroChrom®software V 3.05 or higher
Make sure that:
•There is complete access to all devices and ports on the PC
(e.g. local administrator or administrator).
•The PC’s Ethernet interface is properly installed and activated.
Installation
Connect the detector to the power supply cable.
Installation without LAN (just PC and detector)
Connect the Smartline PDA Detector 2800 to the PC via the crosslinked
Ethernet patch cable (yellow/grey).
In case the preset IP address (172.16.5.241) of the detector cannot be
used please change the default settings according to SOP 1 (see below).
Switch on the PC. Check the PC’s IP address following SOP 2 (see
below). If the PC’s IP address does not fit with the detector’s address
please change the PC’s IP address according to SOP 3 (see below).
Switch on Smartline PDA Detector 2800. The main power switch is on the
rear panel.
Check the communication between PC and Smartline PDA Detector
2800 according to SOP 4.
Installation with LAN (PC is connected to a network)
Connect Smartline PDA Detector 2800 to the network (switch, hub or wall
socket) via the standard Ethernet patch cable (blue).
Knauer stores a default IP address in the Ethernet-Link interface device
of the Smartline PDA Detector 2800. Consult your network administrator
for a unique IP address to integrate in your company's network. Most
likely, you will need to reconfigure the Ethernet-Link interface’s default
settings for IP address, along with subnet mask and gateway, to meet
your network’s specific needs.
In case the preset IP address (172.16.5.241) cannot be used please
change the default settings according to SOP 1 (see below).
Switch on the PC. It is possible to see the PC’s network settings following
SOP 2 (see below). (Please do not change any settings without the
network administrator’s approval!)
Switch on Smartline PDA Detector 2800. The main power switch is on the
rear panel.
Check the communication between PC and Smartline PDA Detector
2800 according to SOP 4.
SOP 1 Displaying / Setting the S2800’s IP address
1. Connect the detector’s serial port to a COM port of the PC using a
standard RS 232 cable (female/female).
2. Start a terminal program (e.g. Windows Hyperterminal), enter the
number of the PC’s COM port and the default settings for data
transfer (data rate 57600, 8 data bits,1stop bit, no parity).

Operating the PDA Detector 2800 11
3. Power up the detector (not just standby!). The current configuration
will be displayed. Fig. 8 is an example of the boot up screen of the
Ethernet-Link interface:
Fig. 8
4. Press <ENTER> before the timeout expires.
5. Press <M> to modify the configuration settings.
6. Change the desired network parameters (IP address, subnet mask
and gateway) by entering each setting followed by pressing
<ENTER>. (Pressing just <ENTER> will confirm the preset value.)
Please note that the setting for DHCP must always be ‘N’.
7. The Etherlink interface settings will be saved and the instrument
will be initialized.
SOP 2 How to find out a PC’s IP address
1. The network configuration of your PC can be viewed by entering
“ipconfig” and pressing <ENTER> from the Command Prompt
(START, Programs, Accessories, Command Prompt).
2. The detector’s IP address and the PC’s IP address should usually
only differ within the last three digits of their IP addresses (on the
right of the third dot). The last three digits must be between 0 and
255. The detector’s IP address and the PC’s IP address should not
be identical.
Table 5 IP addresses – suitable and non suitable combinations
IP address detector
IP address PC
Subnet mask suitable
172.16.5.241 172.16.5.1 255.255.255.0 YES
172.16.5.241 172.16.5.240255.255.255.0 YES
172.16.5.241 172.16.5.241 255.255.255.0 NO
172.16.5.241 172.16.5.242255.255.255.0 YES
172.16.5.241 172.16.1.1 255.255.255.0 NO
SOP 3 How to change a PC’s IP address
From START, Settings, Network and Dial-up
Connections, Local Area Connection, Properties,
Internet Protocol (TCP/IP), Properties you can
change a PC’s IP address and other network
settings.
SOP 4 Communication check
1. Switch off the Smartline PDA Detector 2800.
2. From START, Programs, Accessories, Command Prompt and
then entering “ping” followed by the detector’s IP address

12 Operating the PDA Detector 2800
(e.g. ‘ping 172.16.5.241’) and pressing <ENTER> you should
get a number of timeout messages. If you get a reply instead
of timeout messages there is already a PC or other device
connected that has the same IP address like the Smartline
PDA Detector 2800. In this case please remove that PC or
device or change it’s IP address or change the detector’s IP
address and then repeat this procedure.
3. Switch on the Smartline PDA Detector 2800.
4. From START, Programs, Accessories, Command Prompt and
then entering “ping” followed by the detector’s IP address
(e.g. ‘ping 172.16.5.241’) and pressing <ENTER> you should
get a reply from the detector. If you get timeout messages
instead of a reply there is communication problem between
PC and the Smartline PDA Detector 2800. In this case
please check the PC’s and detector’s IP addresses and the
ethernet patch cable that connect’s the detector with the PC.
Then repeat this procedure.
Installation of the Flow Cell
The PDA Detector 2800 comes factory configured without a flow cell.
Before operating the detector it is necessary to install an appropriate
KNAUER flow cell. A list of available flow cells can be found on page 33.
Fig. 9 Analytical flow cell, 10 mm
SOP 5 Installation of the Flow Cell
1. Swing open the detector’s front door.
2. Referring to Fig. 10, attach the flow cell (3) to the flow cell holder
(1) using the two knurled screws (7) and make sure that the outlet is
on the upper side and that the fixing hole on the back side of the
cell meets the corresponding metal pin (5) of the flow cell holder.
(KNAUER analytical flow cells are marked with ‘I’ at the inlet and ‘O’
at the outlet. For this type of cells inlet and outlet cannot be
exchanged because this would result in increased noise. For all
other cells inlet and outlet are identical.)
3. Now install the two short fiber optical cables (4) connecting the
detector’s and the flow cell’s connectors for fiber optical cables
(2,6). All connections should only be tightened manually.
The flow cell can also be placed outside the detector by just exchanging
the fiber optical cables with longer ones.
For best results, the front door should be closed during operation of the
detector. Flow cells for 1/4” connectors should not be placed in the cell
holder (alternatively the door must be left open).

Operating the PDA Detector 2800 13
1
2
3
4
5
6
7
Fig. 11 Installing a flow cell
The instrument and lamp need not be switched off when exchanging
the flow cell but the lamps should be switched off or the shutter
should be closed. Otherwise, UV light might harm your eyes. The
instrument is ready for immediate operation after proper installation
of a flow cell.
Never touch optical parts (e.g. the optical fiber in the center of the
fiber optical cables’ SMA connectors) with your hands. If it does
happen accidentally, please clean the optical parts with
isopropanol.
Fiber optical cables are very sensitive to bending and may break
easily. They must be handled very carefully and may only be bent as
little as necessary for connecting the flow cell.
A broken fiber optical cable causes very low light intensity which leads to
very high noise and drift.
Since the lifetime of fiber optical cables depends very much on it’s
handling by the user, these cables, as well as lamps, are normally
not covered by any warranty (except where the user can prove that
it is not his fault or that the instrument was already delivered with a
bad fiber optical cable or lamp).
Capillary Connection to an HPLC System
SOP 6 Capillary connections
1. Connect the outlet of the HPLC column to the inlet bushing of the
flow cell (lower side).
2. Push the bushing, the clamping ring, and the sealing ring onto the
capillary (for standard connections just use the respective bushing
and clamping ring). Please take care to note the sequence and
orientation of the fittings according to Fehler! Verweisquelle konnte
nicht gefunden werden.

14 Operating the PDA Detector 2800
3. Push the capillary as far as possible into the flow cell inlet.
4. Fasten the bushing by hand (for standard stainless steel connections
please use the supplied wrench).
5. Connect the flow cell outlet (upper side) using a capillary or PTFE
tube (ID > 0.5 mm) to a waste bottle.
The capillary connections in a simple HPLC system are shown in the
figure below.
Fig. 12 Capillary connections of the detector
(example: low pressure gradient HPLC system)
Use bushings (e.g. DYNASEAL bushings, see Accessories on page
33) which keep the dead volume as small as possible and the
shortest possible capillary with a small internal diameter.
Fig. 13 DYNASEAL capillary connections

Operating the PDA Detector 2800 15
Before taking a measurement cell filled with solvent into operation,
please make certain that the eluent used is miscible with the one
used previously. Otherwise, purge the flow cell with a medium
which is miscible with both of the eluents.
Even though the Smartline PDA Detector 2800 is very resistant to
many kinds of commonly used eluents, you should take care that no
eluent or water come in contact with the instrument’s surface or
with the inside of the instrument. Chlorinated hydrocarbons could
destroy the varnish and some others (e.g. THF) could loosen the
keypad, for example.
Switching on the Detector
Connect the detector to the power supply cable and switch on the instru-
ment. The main power switch is on the rear panel.
After switching ON, the Power and Error LEDs will light.
Choose Ethernet interface and enter the detector’s IP address in the
EuroChrom®or ChromGate®detector setup.
If ChromGate®software is used, the detector’s lamp(s) will be switched
on when a method is opened (Error LED goes off, busy LED is on, UV
LED is on after about 30 seconds, VIS LED is on if halogen lamp is
installed).
If EuroChrom®software is used, the detector’s lamp/s will be switched
on after clicking on ‘Lamp Ctrl’ or ‘Info’ and ‘Reset’ in the detector’s
hardware setup (Error LED will also go off).
Whenever powering up, the instrument includes initialization and check of
the electronics. In addition, the D2lamp is heated to it’s working
temperature for about 20 seconds before it is switched on.
After a period of about 10 to 30 minutes the instrument reaches a
constant working temperature. The baseline is now stabilized and the
PDA detector is ready to acquire data.
Prior to the first measurement wait for about 15 minutes for the
instrument to warm up while the HPLC pump is running (with flow).
In case of especially sensitive measurements, prolonging this warm
up period may be necessary.

16 Software Control of the PDA Detector 2800
Software Control of the PDA Detector 2800
The Smartline PDA Detector 2800 can only be operated when controlled
by a chromatography software (either KNAUER EuroChromfor
Windows or ChromGate).
The Smartline PDA Detector 2800 is supported by EuroChrom®version
3.05 and higher and ChromGateversion 3.1 and higher.
Fig. 14 ChromGate®HPLC software
In combination with the software, all features of the diode array
technology can be used. Depending on the software, functions such as
continuous spectra scanning, 2D chromatograms, 3D chromatograms,
peak purity functionality, spectral library functionality and more are
available.
For KNAUER ChromGate®Software, an additional PDA license is
required.
For more detailed information about the software, it’s features and how to
work with it, please have a look into the particular software manual.
Operating the Detector with ChromGate®
Detector Setup
Fig. 15 KNAUER HPLC System
Choose Detector K-2800 from the available modules in the KNAUER
HPLC System setup and double-click on the detector icon to enter the
configuration menu.

Software Control of the PDA Detector 2800 17
Fig. 16 Detector Configuration
From the Detector Configuration menu it is possible to change settings for:
•Name (default: S-2800)
•Interface: Ethernet or PCI (for S-2800 only Ethernet can be used)
•Y-Axis Units: AU, mAU or µAU (default: mAU)
•Serial Number: The serial number of the detector can be entered
(default: 0)
•IP Address: Here you must enter the Instrument’s IP address
(default: 172.16.5.241)
•Flow Cell: The type of flow cell installed can be entered here
(default: blank)
•D2-Lamp: This option is always activated
•Halogen Lamp: Please activate this option if a halogen lamp is
installed
•Shutter Control: if this box is checked, shutter control is enabled
(default: disabled)
By clicking on OK the changes are saved and the Detector Configuration
menu is closed.
Fig. 17 Configuration Options
If you wish to work in PDA mode (spectra acquisition enabled) the PDA
option must be activated from the Options menu.
(PDA option can only be activated if a PDA license has been previously
installed!)

18 Software Control of the PDA Detector 2800
Settings and Initial Tests
Double click on the instrument in the ChromGate®main window.
Fig. 18 Instrument Setup
When the Instrument is opened by double clicking on it’s icon,
ChromGate®initializes the Smartline PDA Detector 2800 and switches on
the lamp(s). The Error LED goes off.
(The D2lamp first needs about 20 seconds warm up time)
In the Instrument Setup menu (accessible via Method / Instrument Setup)
the method’s main settings can be selected.
•Time constant: 0.1, 0.2, 0.5, 1, 2, 5, 10 sec (default: 0.1 sec)
•Sampling: 0.5, 1, 2, 5 Hz (default: 1 Hz)
•Run Time: (default: 10 Min)
•Acquisition Delay: (default: 0 min)
•Channel: up to four separate channels can be activated for data
acquisition (default: one channel)
•Bandwidth: for each channel bandwidth can be set separately
(default: 1 nm, recommended: 8 nm)
•Detector (Time) Program: for every channel the wavelength can
be programmed separately – the entered wavelengths must be
within the scan range
•Scan (If the PDA option is activated): Start, End and Bandwidth
can be chosen within the shown range (e.g. 190-1025 nm –
default: 200-500 nm)
•Bandwidth: (default: 1 nm, recommended: 1 nm)
•Signal Mode: Absorption / Intensity (default: absorption)
•Acquire 3D data: If this option is not activated only the selected
channels will be recorded – spectra will not be recorded
•Analog output scale: Here you can select which absorption
should represent 10 V output signal on both analog outputs
•Autozero at wavelength change: Here you can decide whether an
autozero should be performed when the wavelength is changed

Software Control of the PDA Detector 2800 19
•Lamp off at the end of run: if lamp off is enabled the lamp(s) will
be switched off at the end of a run (default: disabled)
Fig. 19 Spectrometer Information
By clicking on Info and then on Info again, the Spectrometer Information
from the current detector will be loaded. The individual wavelength range
of this detector will now also be shown as Range in the Instrument Setup
(there is no information about what lamps are installed in the detector).
All parameters are saved together with the current method. This has to
be done with every new method or if the detector is exchanged.
Fig. 20 Select type of synchronization
In the Trigger menu the trigger can be chosen between None (immediate
start), Manual (start after confirming with Enter), External (Trigger must
be applied to a chosen instrument) or Wired (Trigger must be applied to
all instruments). (default: None)
Input defines the instrument which should be connected to the trigger.