Knauer Azura Detector UVD 2.1S User manual

HPLC
Detector UVD 2.1S
Instructions
Document no. V6820

Note: For your own safety, read the instructions and observe the war-
nings and safety information on the device and in the instructions. Keep
the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Phone : [phone] (9–17h Central European Time)
Fax: [phone]
E-Mail: [e-mail]
Languages: German, English
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V6820
Version number: 5.0
Release date: 2019/12/05
Original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library
For the printed versions of our instructions, we use environmentally
friendly paper from sustainable forests.
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2019
All rights reserved.
AZURA® is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.
Customer Support:
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AZURA® Detector UVD 2.1S Instructions, V6820
Table of contents
1. General ...........................................................................1
1.1 About these instructions ..................................................1
1.2 Signal words ............................................................1
1.3 Additional typographical conventions ......................................1
1.4 Legal information ........................................................2
1.4.1 Liability limitation.................................................2
1.4.2 Transport damage ................................................2
1.4.3 Warranty conditions ..............................................2
1.4.4 Declaration of conformity..........................................2
2. Basic safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
2.1 Intended use ............................................................2
2.1.1 Operating ranges ................................................2
2.1.2 Foreseeable misuse...............................................3
2.2 User qualification ........................................................3
2.3 Operator responsibility ...................................................3
2.4 Personal safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.5 Working with solvents ....................................................4
2.5.1 General requirements .............................................4
2.5.2 Contamination by health-threatening solvents ........................4
2.5.3 Avoiding leakage .................................................4
2.6 Specific environments ....................................................5
2.6.1 Earthqake-endangered areas ......................................5
2.6.2 Explosive environment ............................................5
2.6.3 Cooling room ....................................................5
2.7 Maintenance, care and repair..............................................5
2.8 Service request form and decontamination report............................5
3. Product information.................................................................6
3.1 Features ................................................................6
3.2 Flow cells ...............................................................6
3.3 Scope of delivery ........................................................6
3.4 Views ..................................................................7
3.5 Symbols and signs .......................................................8
4. Installation and initial startup ........................................................9
4.1 Unpacking ..............................................................9
4.2 Ambient conditions ......................................................9
4.2.1 Operation site ...................................................9
4.2.2 Ambient temperature .............................................9
4.2.3 Space requirements .............................................10
4.3 Power supply...........................................................10
4.4 Initial startup ...........................................................11
4.4.1 Inserting the flow cell ............................................11

AZURA® Detector UVD 2.1S Instructions, V6820
4.5 Setting the optical path length of a preparative flow cell .....................12
4.6 Connecting capillaries...................................................13
4.7 Switching on the detector................................................13
4.8 Computer control.......................................................15
4.8.1 Setting up LAN..................................................15
4.8.2 Configuring LAN settings.........................................15
4.8.3 Connecting devices with LAN .....................................16
4.8.4 Configuring the router ...........................................16
4.8.5 Integrating LAN into the company network .........................17
4.8.6 Controlling several systems separately in LAN .......................17
4.9 Setting IP addresses via software .........................................18
4.9.1 Mobile Control: Setting a static IP address ..........................18
4.9.2 Mobile Control: Setting a dynamic IP address via device name.........19
4.9.3 Mobile Control: Setting a dynamic IP address via device serial number..19
4.9.4 Firmware Wizard: Setting a static IP address.........................20
4.9.5 Firmware Wizard: Setting a dynamic IP address ......................21
4.10 Remote control .........................................................22
4.10.1 Connector assignment ...........................................22
4.10.2 Connecting the pin header .......................................22
4.10.3 Analog control ..................................................23
4.10.4 Ground ........................................................24
5. Operation ........................................................................25
5.1 Checklist before operation ...............................................25
5.2 Software control ........................................................25
5.2.1 Chromatography software ........................................25
5.2.2 Mobile Control ..................................................25
5.3 Basic operation.........................................................26
5.4 Controlling the device ...................................................26
5.4.1 Menu structure..................................................27
5.4.2 Choosing a control option ........................................28
5.4.3 Checking or editing LAN settings ..................................28
5.4.4 Activating/deactivating the lamp ..................................29
5.4.5 Adjusting the chromatogram......................................29
6. Functionality tests .................................................................32
6.1 Installation Qualification (IQ) .............................................32
6.2 Operation Qualification (OQ) .............................................32
7. Troubleshooting ...................................................................33
7.1 L AN ...................................................................33
7.2 Error messages .........................................................34
8. Maintenance and care..............................................................35
8.1 Maintenance tasks ......................................................35
8.2 Maintenance contract ...................................................35
8.3 Replacing the flow cell...................................................36
8.4 Cleaning and caring for the device ........................................36

AZURA® Detector UVD 2.1S Instructions, V6820
8.5 Rinsing the flow cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
8.5.1 Cleaning the lens of an analytical flow cell ..........................38
8.5.2 Cleaning the light guide of a preparative flow cell....................39
8.6 Replacing the fiber optics ................................................40
9. Transport and storage..............................................................41
9.1 Taking the device out of operation ........................................41
9.2 Packing the device ......................................................41
9.3 Transporting the device .................................................41
9.4 Storing the device ......................................................41
10. Disposal ..........................................................................42
10.1 AVV-Marking Germany ..................................................42
10.2 WEEE registration number ...............................................42
10.3 Eluents and other operating materials .....................................42
11. Technical data.....................................................................43
11.1 Detection..............................................................43
11.2 Communication ........................................................43
11.3 General ...............................................................44
12. Reorders .........................................................................44
12.1 Devices................................................................44
12.2 Sof t ware...............................................................45
12.3 Accessories and spare parts..............................................45
12.4 Fiber optic connectors ..................................................45
12.5 Flow cells ..............................................................45
12.5.1 Analytical flow cells ..............................................45
12.5.2 Preparative flow cells.............................................46
12.5.3 Preparative flow cells - fiber optics .................................47
12.5.4 Test cells .......................................................47
13. Chemical compatibility of wetted materials ...........................................48
13.1 General ...............................................................48
13.2 Plastics ................................................................48
13.3 Non-metals ............................................................50
13.4 Metals.................................................................51
14. Index.............................................................................52

1
AZURA® Detector UVD 2.1S Instructions, V6820
1. General
1.1 About these instructions
These operating instructions enable the safe and efficient operation of
the device. The user must have carefully read and understood these ope-
rating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see „2 Basic safety instructions“, p. 2). In addition to the
safety and warning instructions in these operating instructions, the local
accident prevention regulations and the national industrial safety regulati-
ons apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word "Note” and an
information icon.
Note: This is an example.

2
AZURA® Detector UVD 2.1S Instructions, V6820
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see „2.2 User quali-
fication“, p. 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unaut-
horized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity
2. Basic safety instructions
The device has been developed and constructed in such a way that ha-
zards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used for chromatographic applications in
the laboratory.

3Basic safety instructions
AZURA® Detector UVD 2.1S Instructions, V6820
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outside of a laboratory or measurement room. Otherwise,
the manufacturer does not guarantee the functionality and safety of
the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The user is qualified to handle the device if all of the following points
apply:
He has at least a basic knowledge of liquid chromatography.
He has knowledge about the properties of the used solvents and their
health risks.
He is trained for the special tasks and activities in the laboratory and
knows the relevant standards and regulations.
Due to his technical training and experience, he can understand and
carry out all the work described in the operating instructions on the
instrument and recognize and avoid possible dangers independently.
His ability to react is not impaired by the consumption of drugs,
alcohol or medication.
Participation in the installation of a device or a training by the company
KNAUER or an authorized company.
If the user does not meet these qualifications, he must inform his
supervisor.
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and un-
derstood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).

4Basic safety instructions
AZURA® Detector UVD 2.1S Instructions, V6820
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective afety equipment which is specified in the parti-
cular laboratory.
2.5 Working with solvents
2.5.1 General requirements
The user is trained for handling different solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
„13 Chemical compatibility of wetted materials“, p. 48).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
2.5.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see „10 Disposal“, p. 42).
2.5.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch off the system. Only take the device into
operation if the cause of the leak has been resolved (see „8 Mainte-
nance and care“, p. 35).

5Basic safety instructions
AZURA® Detector UVD 2.1S Instructions, V6820
2.6 Specific environments
2.6.1 Earthqake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.
2.6.2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2.6.3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.7 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer's instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see „1.4.1 Liability limitation“, p. 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting
2.8 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest

6
AZURA® Detector UVD 2.1S Instructions, V6820
3. Product information
3.1 Features
One of the smallest HPLC detectors on the market.
A wide range of flow cells for analytical or preparative LC applications
with flow rates from 10 µl/min up to 10 l/min.
Automatic recognition and storage of device-specific information,
which are important for Good Laboratory Practice, Operation Qualifi-
cations (OQ), for repairing the device.
Automatic and current diagnosis of the device.
Control with chromatography software or with the front panel.
Easy integration of the detector into complex chromatography
systems.
Wavelength change via analog possible.
3.2 Flow cells
Various types of flow cells are available to the user. Pay attention to the
compatability of the flow cells. See „12. Reorders“ on page 44.
Note: A test cell is preassembled to the device. Before the detector can
be used for measurements the test cell has to be replaced by a flow cell.
The UVD 2.1S Fiber Optics Version is delivered without a test cell.
3.3 Scope of delivery
The following items should be included in the scope of delivery:
Device AZURA® Detector UVD 2.1S with test cell (test cell not supplied
with Fiber Optics Version)
Power cable
Power supply unit
AZURA® UVD 2.1 accessory kit
AZURA® accessory kit
Transport protection
Valid documents:
Instructions
Installation Qualification (“IQ”)
Declaration of Conformity

7Product information
AZURA® Detector UVD 2.1S Instructions, V6820
3.4 Views
Front view
Legend
1
Display
2
Keyboard
3
Flow cell
3
12
Fig.1 Front view
Rear view
Legend
1
RS-232 port
2
LAN port
3
Pin header
4
Integrator output
5
Connection
6
Ground
7
Fan
Fig.2 Rear view
7
6
5
4
3
2
1

8Product information
AZURA® Detector UVD 2.1S Instructions, V6820
3.5 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
High-voltage hazard
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
A device marked with CE fulfills the product spe-
cific requirements of European directives. This is
confirmed in a Declaration of Conformity.

9
AZURA® Detector UVD 2.1S Instructions, V6820
4. Installation and initial startup
Before you determine the operation site, read chapter 11 on page 43.
There you will find all device-specific information on power supply, am-
bient conditions and humidity.
Note: The intended use be ensured only if the requirements for ambient
conditions of the operating environment are met.
4.1 Unpacking
Process
1. Place the packaging in such a way that the lettering on the label is
in the correct position.
2. Check the packaging, the device and the accessories for transport
damage.
3. Check the scope of delivery. In the event of incomplete delivery,
contact Technical Support immediately.
4. When lifting, carrying or moving the device, grab the unit only from
below on the sides. Do not hold onto front cover or leak tray, as
these parts are loosely attached to the device.
Keep the included packing list for repeat orders.
Keep the original packaging for safe storage or transportation.
4.2 Ambient conditions
4.2.1 Operation site
Observe the following requirements for the operation site so that the
measurement results are not influenced:
Place on a firm, level and straight surface.
Protect against direct sunlight.
Do not expose to air drafts such as air conditioning systems.
Do not set up the to other machines that cause floor vibrations.
Keep from high frequency sources.
Ensure adequate ventilation (see „4.2.3 Space requirements“, p. 10).
Avoid temperature fluctuations. (see „4.2.2 Ambient temperature“, p.
9)
4.2.2 Ambient temperature
If the ambient temperature of the device is abruptly changed (e.g. when
it is installed in a cooling chamber), condensation will form inside the
device and may cause damage to the device. Allow the device to accli-
mate for 3 h, before it is connected to the power supply and taken into
operation.
Procedure
Next steps

10Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
4.2.3 Space requirements
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnec-
ted from the power supply.
Ensure adequate ventilation around the device, otherwise it may over-
heat and malfunction:
- Min. 5 cm distance if another device is set on one side.
- Min. 10 cm distance if further devices are set on both sides.
- At least 15 cm to the cooler fan on the rear.
The mounting of a small device to an AZURA L device with a mounting
bracket does not affect the performance of either device. The space
requirements specified in both device instructions do not apply in this
case.
4.3 Power supply
Power supply requirements
Failure-free power supply: For failure-free operation, the electrical
voltage must be free of fluctuations, residual currents, voltage peaks
and electromagnetic interference. The device must receive sufficient
voltage and reserve capacity.
Check voltage: Only connect devices to a power supply whose voltage
corresponds to the permissible voltage of the device.
Power consumption: The nominal power of the connected devices
must not exceed 50 % of the highest connected power capacity, since
higher currents can flow briefly when the device is switched on.
Main connection: The electrical power supply at the operation site
must be connected directly to the nearest main power connection.
Grounding: The connectors for the voltage must be grounded
accordingly.
Power supply cables and plugs
Original parts: For power supply, use the supplied power cable and
plug to meet the specifications which are described in the chapter 11
on page 43. Detachable power cable cables are not allowed to be
replaced with other cable types.
Country-specific plugs: Before switching on the device, check whether
the supplied plug is approved for your country. Overview of the de-
vice- and country-specific plug types from KNAUER:
www.knauer.net/plugs
Power strips: If several devices are connected to one power strip, al-
ways consider the maximum power consumption of each device.
Access to power supply: Make sure that the power plug on the pow-
er supply (wall socket or power strip) is always accessible, so that the
device can be disconnected easily from the power supply.
Damaged power supply cables and plugs: For safety reasons, dama-
ged or faulty cables and plugs must not be used to connect the device
to the power supply. Replace defective cables and plugs only with
KNAUER accessories.

11 Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
4.4 Initial startup
Note: Before the detector is ready for use, a flow cell must be installed.
Before installing the flow cell, the optical path length in the flow cell can
be changed. The optimal path length depends on the type and the quan-
tity of the sample.
4.4.1 Inserting the flow cell
Note: The test cell is used during operation qualifications and must be
stored.
Detector is switched off.
Power plug is disconnected from the device.
Steps Figure
1. Unscrew the 2 knur-
led-head screws 1.
Fig.3 Removing the slide
2. Pull out the slide 2.
3. Remove the test cell.
4. Insert the flow cell 4
into the slide.
Fig.4 Assembling the flow cell
5. Push the slide 3into
the detector.
6. Insert the knurled-he-
ad screws and screw
tight.
The flow cell is assembled.
Connect the capillaries.
Prerequisite
Procedure
1
2
4
3
Result
Next step

12Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
4.5 Setting the optical path length of a prepa-
rative flow cell
Depending on type, the path length is factory set to 2 mm, 3 mm, or
10 mm. For the 2 mm flow cells you can remove the spacers on one or
both sides and hence change the path length to 1.25 mm or 0.5 mm.
Legend
1
Threaded ring
2
Cover
3
Spacer
4
Seal holder (com-
pression bushing)
5
Light guide with
seal ring
Fig. 5: Preparative flow cell
Flow cell is removed from the detector.
Allen screwdriver, size 3
Process
1. Using the allen screwdriver, loosen the threaded ring 1.
2. Remove the cover 2.
3. To shorten the optical path length, remove the spacer 3.
To enlarge the optical path length, put in one or both spacers.
4. Insert the cover 2.
5. Using the allen screwdriver, tighten the threaded ring 1.
The optical path length is set.
Mount the flow cell onto the detector.
Eye injury
Eye irritation by UV light. Bundled UV light can escape from the flow
cell or fiber optic cables.
Switch off the detector and disconnect it from the power supply.
1 2 4
35
Prerequisite
Tools
Procedure
Result
Next steps

13 Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
4.6 Connecting capillaries
Note: PEEK fittings withstand a maximum pressure of 400 bar.
Flow cell is removed from the detector.
Torque wrench
Steps Figure
1. Push the capillary 2
through the fitting 1.
Fig.6 Capillary fitting
2. Push the clamping ring
3onto capillary.
3. Manually, screw toge-
ther the capillary 5
and the flow cell 4.
Tighten the fitting with
the wrench.
Fig.7 Capillary and flow cell
The capillary is connected.
4.7 Switching on the detector
Installation has been completed.
Flow cell is mounted on the detector.
Power supply unit is switched off.
Component defect
Damage to components due to excessive tightening possible.
Observe the torque of the screw connection
Use 5Nm torque for stainless steel fittings.
Use 1Nm torque for PEEK fittings.
Prerequisite
Tools
Procedure 2 31
5
4
Result
Device defect
Changes of the environmental temperature cause condensation inside
the device.
Allow device to acclimate for 3h before connecting to power supply
and taking into operation.
Prerequisite

14Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
Process
1. Connect the power cable with the device.
2. Connect the power supply unit with the power cable..
3. Connect the power supply unit with the power supply.
4. Using the power supply switch, switch on the detector.
The detector starts its self-calibration. The display shows the progress that
has been made. When the self-calibration has finished, the status display
appears. If the self-calibration fails, switch the detector off and back on.
Procedure
Result

15 Installation and initial startup
AZURA® Detector UVD 2.1S Instructions, V6820
4.8 Computer control
Note: HPLC devices from KNAUER only work with IP addresses, which
have been assigned by IPv4. IPv6 is not supported.
This chapter describes how to set up a chromatography system in a local
area network (LAN) and how a network administrator can integrate this
LAN into your company network. The description applies to the operating
system Windows® and all conventional routers.
Note: When using PurityChrom®, static IP addresses are needed
(see chapter 4.8 on page 15).
4.8.1 Setting up LAN
To set up a LAN, we recommend to use a router. That means the following
steps are required:
Process
1. On the computer, go to the control panel and check the LAN
properties.
2. Hook up the router to the devices and the computer.
3. Set up the router for the computer network.
4. Install the chromatography software.
5. Switch on the device and run the chromatography software.
Configure LAN properties (see section 4.8.2).
4.8.2 Configuring LAN settings
The LAN uses only one server (which is normally the router) from that the
devices automatically receive their IP address.
In Windows, power saving, hibernation, standby, and screen saver
must be deactivated.
In case you use an USB-to-COM box, the option "Allow the computer
to turn off this device to save power" in the device manager must be
deactivated for all USB hosts.
Applies to all LAN devices: Disable the setting for the network adapter
in Device Manager "Allow the computer to turn off this device to save
power”.
Process
1. In Windows, open <Network and Sharing Center>.
2. Double-click on <LAN connection>.
3. Click on the button <Properties>.
4. Select <Internet Protocol version4 (TCP/IPv4)>.
5. Click on the button <Properties>.
Procedure
Next steps
Prerequisites
Procedure