Knauer Azura DAD 6.1L User manual

HPLC
Detector DAD 6.1L/DAD 2.1L/MWD 2.1L
Instructions
Document No. V6700

Note: For your own safety, read the instructions and observe the war-
nings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V6700
Version number: 4.3
Release date: 2020/02/10
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library.
For the printed versions of our instructions, we use environmentally
friendly paper from sustainable forests.
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2020
All rights reserved.
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KNAUER Wissenschaftliche Geräte GmbH.
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1
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
Table of contents
1. General ...........................................................................1
1.1 About these instructions..................................................1
1.2 Signal words ............................................................1
1.3 Additional typographical conventions ......................................1
1.4 Legal information ........................................................2
1.4.1 Liability limitation.................................................2
1.4.2 Transport damage ................................................2
1.4.3 Warranty conditions ..............................................2
1.4.4 Warranty seal ....................................................2
1.4.5 Declaration of conformity..........................................2
2. Basic safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.1 Intended use ............................................................3
2.1.1 Operating ranges ................................................3
2.1.2 Foreseeable misuse...............................................3
2.2 User qualification ........................................................3
2.3 Operator responsibility ...................................................4
2.4 Personal safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.5 Safety features on the device ..............................................4
2.6 Working with solvents ....................................................5
2.6.1 General requirements.............................................5
2.6.2 Contamination by health-threatening solvents ........................5
2.6.3 Avoiding leakage .................................................5
2.7 Specific environments ....................................................6
2.7.1 Earthqake-endangered areas ......................................6
2.7.2 Explosive environment ............................................6
2.7.3 Cooling room ....................................................6
2.8 Maintenance, care and repair..............................................6
2.9 Service request form and decontamination report............................6
3. Product information.................................................................7
3.1 Features ................................................................7
3.2 Scope of delivery ........................................................7
3.3 Views ..................................................................7
3.4 Performance overview....................................................8
3.5 Symbols and signs ......................................................10
4. Installation and initial startup .......................................................11
4.1 Unpacking .............................................................11
4.2 Ambient conditions .....................................................11
4.2.1 Operation site ..................................................11
4.2.2 Ambient temperature ............................................11
4.2.3 Space requirements .............................................12
4.3 Power supply...........................................................12

2
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
4.4 Connecting the leak management ........................................13
4.5 Inserting the flow cell....................................................14
4.5.1 Inserting the flow cell with fiber optics..............................15
4.6 Connecting the capillaries ...............................................16
4.7 Computer control.......................................................17
4.7.1 Setting up LAN..................................................17
4.7.2 Configuring LAN settings.........................................17
4.7.3 Connecting devices with LAN .....................................18
4.7.4 Configuring the router ...........................................18
4.7.5 Integrating LAN into the company network .........................19
4.7.6 Controlling several systems separately in LAN .......................19
4.8 Setting IP addresses via software .........................................20
4.8.1 Mobile Control: Setting a static IP address ..........................20
4.8.2 Mobile Control: Setting a dynamic IP address via device name.........21
4.8.3 Mobile Control: Setting a dynamic IP address via device serial number..21
4.8.4 Firmware Wizard: Setting a static IP address.........................22
4.8.5 Firmware Wizard: Setting a dynamic IP address ......................23
4.8.6 Remote control..................................................24
4.8.7 Connections to the terminal strip ..................................25
4.8.8 Connecting the pin header .......................................26
4.8.9 Integrator connector .............................................27
4.9 Integrating the detector into the system ...................................28
4.10 Pre-Installed capillaries ..................................................28
5. Operation ........................................................................29
5.1 Initial startup ...........................................................29
5.2 Switch on ..............................................................29
5.3 Software operation .....................................................30
5.3.1 Control methods ................................................30
5.3.2 Operating with Mobile Control ....................................30
5.4 Meaning of the LEDs ....................................................31
5.5 Default settings.........................................................32
5.6 GLP ..................................................................32
6. Optimizing the detector ............................................................34
6.1 General ...............................................................34
6.2 Selecting the flow cell ...................................................34
6.3 Selecting the wavelength ................................................37
6.3.1 Bandwidth......................................................37
6.4 Spectral range .........................................................38
6.5 Time constant & data rate................................................38
6.6 Integration time ........................................................39
6.7 Subtraction of the baseline chromatogram .................................40
6.7.1 Extended linear range ...........................................40
7. Functionality tests .................................................................41
7.1 Installation Qualification (IQ) .............................................41
7.2 Operation Qualification (OQ) .............................................41

3
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
8. Troubleshooting ...................................................................42
8.1 LAN...................................................................42
8.2 Possible problems and solutions ..........................................43
8.3 System messages .......................................................44
9. Maintenance and care..............................................................47
9.1 General ...............................................................47
9.1.1 Maintenance tasks performed by the user...........................47
9.1.2 Maintenance contract ............................................48
9.1.3 Maintenance intervals ............................................48
9.2 Cleaning and caring for the device ........................................48
9.2.1 Handling the flow cell cartridge ...................................48
9.2.2 Surfaces........................................................49
9.3 Cleaning the flow cell....................................................49
9.3.1 Basic cleaning...................................................49
9.3.2 Advanced cleaning ..............................................50
9.3.3 Cleaning protocol for KNAUER LightGuide Flow cells.................51
9.4 Replacing the flow cell...................................................52
9.5 Replacing the lamps.....................................................53
9.5.1 Removing the deuterium or halogen lamp ..........................54
9.5.2 Installing the deuterium or halogen lamp ...........................55
9.6 Checking the fittings ....................................................56
9.7 Removing a leak ........................................................56
9.8 Removing a leakage.....................................................57
9.9 Decommissioning.......................................................57
10. Transport and storage..............................................................58
10.1 Taking the device out of operation ........................................58
10.2 Packing the device ......................................................58
10.3 Transporting the device .................................................58
10.4 Storing the device ......................................................58
11. Disposal ..........................................................................59
11.1 AVV-Marking Germany ..................................................59
11.2 WEEE registration number ...............................................59
11.3 Eluents and other operating materials .....................................59
12. Technical data.....................................................................60
12.1 DAD6.1L ..............................................................60
12.2 DAD2.1L ..............................................................61
12.3 MWD 2.1L..............................................................62
12.4 Conditions for specification ..............................................64
13. Reorders .........................................................................64
13.1 Devices and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
13.2 Flow cells ..............................................................65
13.3 Flow cells with fiber optics ...............................................66

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AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
13.4 Fiber optic cables.......................................................68
14. Chemical compatibility of wetted materials ...........................................69
14.1 General ...............................................................69
14.2 Plastics ................................................................69
14.3 Non-metals ............................................................71
14.4 Metals.................................................................72
15. Index.............................................................................73

1
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
1. General
1.1 About these instructions
These operating instructions enable the safe and efficient operation of
the device. The user must have carefully read and understood these ope-
rating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see „2 Basic safety instructions“, p. 3). In addition to the
safety and warning instructions in these operating instructions, the local
accident prevention regulations and the national industrial safety regulati-
ons apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word "Note” and an
information icon.
Note: This is an example.

2General
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see „2.2 User quali-
fication“, p. 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unaut-
horized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is affixed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician affixes an orange seal in the same pla-
ce. If unauthorised persons tamper with the device or if the seal is dama-
ged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

3
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
2. Basic safety instructions
The device has been developed and constructed in such a way that ha-
zards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used for chromatographic applications in
the laboratory.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outside of a laboratory or measurement room. Otherwise,
the manufacturer does not guarantee the functionality and safety of
the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The user is qualified to handle the device if all of the following points
apply:
He has at least a basic knowledge of liquid chromatography.
He has knowledge about the properties of the used solvents and their
health risks.
He is trained for the special tasks and activities in the laboratory and
knows the relevant standards and regulations.
Due to his technical training and experience, he can understand and
carry out all the work described in the operating instructions on the
instrument and recognize and avoid possible dangers independently.
His ability to react is not impaired by the consumption of drugs,
alcohol or medication.
Participation in the installation of a device or a training by the company
KNAUER or an authorized company.
If the user does not meet these qualifications, he must inform his
supervisor.

4Basic safety instructions
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and un-
derstood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective safety equipment which is specified in the
particular laboratory.
2.5 Safety features on the device
Power switch: Devices of the AZURA® L series may be switched off
using the power switch (toggle switch on the back side of housing) at
any time, this causes no damage to the device. To switch off devices of
the AZURA® S series, remove the plug from the power socket.
Front cover: Devices of the AZURA® L series have a front cover as a
splash protection for the user
Leak tray: Devices of the AZURA® L series have a leak tray on the front
side. The leak tray collects leaking solvents and protects components
from potential damage caused by discharging liquid.
Lamp: For the detectors AZURA DAD 2.1L, DAD 6.1L und MWD 2.1L,
the lamp switches off automatically when the cover is opened.

5Basic safety instructions
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
2.6 Working with solvents
2.6.1 General requirements
The user is trained for handling different solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
„14 Chemical compatibility of wetted materials“, p. 69).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
2.6.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see „11 Disposal“, p. 59).
2.6.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch off the system. Only take the device into
operation if the cause of the leak has been resolved (see „9 Mainte-
nance and care“, p. 47).

6Basic safety instructions
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
2.7 Specific environments
2 .7.1 Earthqake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.
2 .7. 2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2 .7. 3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.8 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer's instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see „1.4.1 Liability limitation“, p. 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting
2.9 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest

7
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
3. Product information
3.1 Features
The AZURA® detector DAD 6.1L/DAD 2.1L/MWD 2.1L (further on referred
to as “detector“ in general, or “DAD 6.1L“,“DAD 2.1L“, and “MWD 2.1L“)
can be used for analytical, semi-preparative and preparative applications.
3.2 Scope of delivery
The following items should be included in the scope of delivery:
Detector with test cell
Power supply cable
Accessories Kit AZURA® Detector DAD 6.1L/DAD 2.1L/MWD 2.1L
Accessories Kit AZURA®
Valid documents:
Instructions
Installation Qualification (“IQ”)
Declaration of Conformity
3.3 Views
Front view
On the front of the detector, there are the flow cell and the removable
lamp cover for lamp replacement.
Legend
1 Leak tray
2 Capillary guide
3 Lamp cover
4 Serial number
5 Switch/standby key
6 Status LED
7 Flow cell
7
6
2
2
3
2
4
1
5
Fig.1 Front view

8Product information
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
Rear view
On the rear of the detector, the connectors for LAN and power plug are
located, as well as the serial number of the device and the power switch
(on/off).
Please note that the service interface is solely used for repair and main-
tenance tasks performed by service technicians. 3D data acquisition is not
fully supported via this interface.
Legend
1 Integrator outputs
2 LAN connector
3 Service interface
4 Multi-pin connector
5 Fan
6 Power connection
and power switch
2
13 5 64
Fig.2 Rear view
3.4 Performance overview
In an HPLC system, the detector serves to detect substances in liquids
and determine their concentration. The sensitivity of the detector de-
pends on the used flow cell.
Legend
1 Deuterium lamp
2 Halogen lamp
3 Flow cell
4 Diode array sensor
5 Diraction grating
6 Mirror
3
5
1
2
4
6
Fig.3 Optical system DAD6.1L
RS-232,
service interface

9Product information
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
The detectors measure the light absorption of the sample in the ultravio-
let and visual spectra. The entire spectrum permeates the sample and
afterwards is split up by a grate. The split light falls onto a geometric array
of 1024 (DAD6.1L) or 256 (DAD2.1L, MWD2.1L) separate photodiodes–
the photodiode array.
The optical system of the DAD6.1L is depicted in figure 3. An active tem-
perature control with 0.5°C sensitivity ensures baseline stability for mini-
mal baseline drift.
You can exchange the lamps quickly and easily without the need of a ser-
vice technician (see section „9.5 Replacing the lamps“ on page 53).
DAD6.1L: Two light sources, a deuterium lamp and a halogen lamp,
cover the entire UV-Vis wavelength range from 190 to 1000 nm.
DAD2.1L, MWD2.1L: One deuterium lamp covers the UV wavelength
range from 190 to 700 nm.
Maximum light intensity over the entire UV-VIS range is provided by effec-
tively mixing the light sources from the deuterium and halogen lamps
with a mirror using Polka Dot technology.
Various types of flow cells allow a wide range of applications. Flow cell
cartridges with KNAUER PressureProof and KNAUER LightGuide techno-
logy as well as KNAUER Fiber Optics Technology are available (see chap-
ter„13. Reorders“ on page 64).
KNAUER LightGuide Flow Cell Cartridges combine a maximum light
transmission (using total reflection) with a minimum cell volume to
offer an ideal signal-to-noise ratio.
KNAUER PressureProof Flow Cell Cartridges are optimized for conven-
tional and high flow HPLC and FPLC applications. These flow cells fea-
ture an increased pressure stability (up to300 bar) and extended flow
rate range (up to 20ml/min).
KNAUER Fiber Optics Technology: Adapters for fiber optics are optio-
nally available. Fiber optics allow separating the flow cell from the de-
tector. This way, working with dangerous, explosive, or toxic materials
becomes safer. Remote flow cells are recommended for preparative
applications (high flow rates), in order to protect sensitive optical com-
ponents from potential leakages.
The detector measures with a maximum data rate of 100Hz. During the
measurement, up to 100 spectra per second are recorded.
Integrated leak management drains liquids from leaks.
You can use the Mobile Control and the different software products (Clari-
tyChrom®, OpenLAB®, or Chromeleon™) to display or read GLP data like
operating hours, lamp operating hours, number of lamp ignitions, etc.
You find a detailed description on how to display or read out GLP data in
the respective software instructions.
The multiwavelength detector can be upgraded to a diode array detector.
Optical module
Lamps
Mirror DAD6.1L
Flow cell
Data rate
Leak management
GLP data
Upgrade MWD 2.1L

10 Product information
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
3.5 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
High-voltage hazard
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
Obey maximum load for leak tray during transpor-
tation, installation and operation.
A device marked with CE fulfills the product spe-
cific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally
recognized testing centers (NRTL). The certified
device or system has successfully passed the qual-
ity and security tests.
A warranty seal is affixed to some devices. For
more information see section 1.4.4 on page 2.

11
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
4. Installation and initial startup
Before you determine the operation site, read the chapter “Technical
Data” (see chapter 12 on page 60). There you will find all device-speci-
fic information on power supply, ambient conditions and humidity.
Note: The intended use be ensured only if the requirements for ambient
conditions of the operating environment are met.
4.1 Unpacking
Process
1. Place the packaging in such a way that the lettering on the label is
in the correct position.
2. Check the packaging, the device and the accessories for transport
damage.
3. Check the scope of delivery. In the event of incomplete delivery,
contact Technical Support immediately.
4. When lifting, carrying or moving the device, grab the unit only
from below on the sides. Do not hold onto front cover or leak tray,
as these parts are loosely attached to the device.
Keep the included packing list for repeat orders.
Keep the original packaging for safe storage or transportation.
4.2 Ambient conditions
4.2.1 Operation site
Observe the following requirements for the operation site so that the
measurement results are not influenced:
Place on a firm, level and straight surface.
Protect against direct sunlight.
Do not expose to air drafts such as air conditioning systems.
Do not set up the to other machines that cause floor vibrations.
Keep from high frequency sources.
Ensure adequate ventilation (see 4.2.3 on page12 ).
Avoid temperature fluctuations. (see 4.2.2 on page11 )
4.2.2 Ambient temperature
If the ambient temperature of the device is abruptly changed (e.g. when
it is installed in a cooling chamber), condensation will form inside the
device and may cause damage to the device. Allow the device to accli-
mate for 3 h, before it is connected to the power supply and taken into
operation.
Procedure
Next steps

12 Installation and initial startup
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
4.2.3 Space requirements
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnec-
ted from the power supply.
Ensure adequate ventilation around the device, otherwise it may over-
heat and malfunction:
- Min. 5 cm distance if another device is set on one side.
- Min. 10 cm distance if further devices are set on both sides.
- At least 15 cm to the cooler fan on the rear.
The mounting of a small device to an AZURA L device with a mounting
bracket does not affect the performance of either device. The space
requirements specified in both device instructions do not apply in this
case.
4.3 Power supply
Power supply requirements
Failure-free power supply: For failure-free operation, the electrical
voltage must be free of fluctuations, residual currents, voltage peaks
and electromagnetic interference. The device must receive sufficient
voltage and reserve capacity.
Check voltage: Only connect devices to a power supply whose voltage
corresponds to the permissible voltage of the device.
Power consumption: The nominal power of the connected devices
must not exceed 50 % of the highest connected power capacity, since
higher currents can flow briefly when the device is switched on.
Main connection: The electrical power supply at the operation site
must be connected directly to the nearest main power connection.
Grounding: The connectors for the voltage must be grounded
accordingly.
Power supply cables and plugs
Original parts: For power supply, use the supplied power cable and
plug to meet the specifications which are described in the chapter
“Technical Data” (see chapter 12 on page 60). Detachable power
cable cables are not allowed to be replaced with other cable types.
Country-specific plugs: Before switching on the device, check whether
the supplied plug is approved for your country. Overview of the de-
vice- and country-specific plug types from KNAUER:
www.knauer.net/plugs
Power strips: If several devices are connected to one power strip, al-
ways consider the maximum power consumption of each device.
Access to power supply: Make sure that the power plug on the pow-
er supply (wall socket or power strip) is always accessible, so that the
device can be disconnected easily from the power supply.
Damaged power supply cables and plugs: For safety reasons, dama-
ged or faulty cables and plugs must not be used to connect the device
to the power supply. Replace defective cables and plugs only with
KNAUER accessories.

13Installation and initial startup
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
4.4 Connecting the leak management
The leak management consists of the leak sensor and the drainage sys-
tem. The drainage system ensures that escaping liquids flow into a waste
bottle. If there is too much liquid, the red LED starts flashing. Both the de-
vice and the data acquisition via chromatography software are stopped.
Remove the front cover.
Procedure Process Figure
1. Carefully push the funnel 1
into the center opening of
the capillary guide 2.
12
Fig. 4 Funnel and capillary guide
2. Push the long ending of the
first nozzle 4into the hose
3.
3
4
Fig. 5 Hose and nozzle
3. Afterwards, push the nozzle
onto the funnel.
4. Push the other end of the
hose onto the nozzle 5of the
leak tray.
5
Fig. 6 Hose connected to device
5. Attach the waste nozzle 6to
the bottom unit
6. Attach the wastehose o the
waste nozzle and connect it
to the waste container.
7. Place the waste container
below the devices.
6
Fig. 7 Leak tray with nozzle
Attach the front cover.
Prerequisite
Next step

14 Installation and initial startup
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
4.5 Inserting the flow cell
Note: Pay attention to the compatibility of the flow cell.
Before taking a flow cell filled with solvent into operation, please make
certain that the used eluent is miscible with that one used previously. Ot-
herwise purge the flow cell with a medium miscible with both the eluents.
No flow cell or test cell is installed.
The device is switched off.
Unscrew the capillary for easier handling.
Eye injury
Irritation of retina through UV light. High-energy UV light can leak out
from the flow cell or the fiber optic connectors.
Switch o the detector or the lamps.
Performance decrease
Any components in the light path like fiber optic ends become
contaminated when touched with fingers.
Avoid touching the ends without gloves.
Use alcohol and a cotton bud for cleaning.
Process Step Figure
Place the flow cell into the ope-
ning and slide to the back until it
clicks into place inside the device.
Fig. 8 Flow cell
Connect the capillaries.
Prerequisites
Practical Tip
Next step