Knauer BlueShadow Detector 50D User manual

Detector 50D
Instructions
Document no. V7659
HPLCHPLC

Note: For your own safety, read the instructions and follow the warnings
and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V7659
Version number: 1.0
Release date: 2020/12/04
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library.
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2021
All rights reserved.
Support:
Publisher:
Version information:
Copyright:

i
BlueShadow Detector 50D Instructions V7659
Table of Contents
1. General ..............................................................................3
1.1 About these instructions.........................................................3
1.2 Signal words ...................................................................3
1.3 Additional typographical conventions .............................................3
1.4 Legal information...............................................................4
1.4.1 Liability limitation......................................................4
1.4.2 Transport damage .....................................................4
1.4.3 Warranty conditions ...................................................4
1.4.4 Declaration of conformity...............................................4
2. Basic safety instructions ................................................................5
2.1 Intended use...................................................................5
2.1.1 Operating ranges .....................................................5
2.1.2 Foreseeable misuse....................................................5
2.2 User qualification ...............................................................5
2.3 Operator responsibility..........................................................6
2.4 Personal safety equipment .......................................................6
2.5 Working with solvents ...........................................................7
2.5.1 General requirements..................................................7
2.5.2 Contamination by health-threatening solvents.............................7
2.5.3 Avoiding leakage......................................................7
2.6 Specific environments...........................................................8
2.6.1 Earthquake-endangered areas ..........................................8
2.6.2 Explosive environment .................................................8
2.6.3 Cooling room .........................................................8
2.6.4 Wet room ............................................................8
2.7 Maintenance, care and repair ....................................................8
2.8 Service request form and decontamination report ..................................9
3. Product information ..................................................................10
3.1 Features ......................................................................10
3.2 Scope of delivery ..............................................................11
3.3 Views ........................................................................11
3.3.1 Front view ...........................................................11
3.3.2 Rear view ............................................................12
3.4 Symbols and signs .............................................................12

Table of contents
BlueShadow Detector 50D Instructions V7659
ii
4. Installation and initial startup ..........................................................13
4.1 Unpacking....................................................................13
4.2 Ambient conditions ............................................................13
4.2.1 Operation site........................................................13
4.2.2 Ambient temperature .................................................13
4.2.3 Space requirements ..................................................14
4.3 Power supply .................................................................14
4.4 Inserting the flow cell ..........................................................15
4.4.1 Changing the optical path length of the preparative flow cell...............16
4.5 Connecting capillaries .........................................................18
4.6 Computer control .............................................................19
4.6.1 Setting up LAN.......................................................19
4.6.2 Configuring LAN settings..............................................20
4.6.3 Connecting devices with LAN ..........................................20
4.6.4 Configuring the router ................................................21
4.6.5 Integrating LAN into the company network ..............................21
4.6.6 Controlling several systems separately in LAN............................22
4.7 Remote control................................................................23
4.7.1 Connector assignment ................................................23
4.7.2 Connecting the pin header ............................................25
4.8 Analog control ................................................................26
4.9 Integrator Port.................................................................26
4.10 Switching on ..................................................................26
5. Operation ...........................................................................27
5.1 Initial operation ...............................................................27
5.2 Software control ...............................................................27
5.2.1 Switching the detector on .............................................27
5.2.2 Controlling with display and keypad ....................................27
5.2.3 LED status ...........................................................28
5.2.4 Operating with the keypad ............................................28
5.2.5 Switch-on and self-test ................................................29
5.3 Selecting a wavelength.........................................................30
5.3.1 Options for signal output ..............................................30
5.4 Menu structure ................................................................31
5.4.1 Main menu ..........................................................31
5.4.2 Menu structure: overview..............................................32
5.4.3 Device setting parameters .............................................33
5.5 GLP menu ....................................................................37
5.6 Program menu ................................................................38

Table of contents
BlueShadow Detector 50D Instructions V7659
iii
5.6.1 Creating a program ...................................................39
5.6.2 Running a program ...................................................40
5.6.3 Changing a program..................................................41
5.6.4 Deleting a program line ...............................................41
5.6.5 Deleting a program ...................................................41
5.6.6 Creating and running a program with preselectable start time
(Wake-Up Program) ...................................................42
5.7 Device Links ..................................................................43
5.7.1 Creating a Link .......................................................44
5.7.2 Running a Link .......................................................45
5.7.3 Changing a Link ......................................................46
5.7.4 Deleting link lines ....................................................46
5.7.5 Deleting a Link .......................................................46
5.8 Scan menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
5.8.1 Autozero scan........................................................47
5.8.2 Wavelength scan .....................................................47
6. Functionality tests ....................................................................48
6.1 Installation Qualification (IQ) ....................................................48
6.2 Operation Qualification (OQ) ...................................................48
7. Troubleshooting......................................................................49
7.1 LAN..........................................................................49
7.2 Possible problems and solutions.................................................50
7.3 System messages..............................................................51
8. Maintenance and care ................................................................54
8.1 Maintenance contract ..........................................................54
8.2 Cleaning and caring for the device...............................................55
8.3 Cleaning the flow cell ..........................................................55
8.3.1 Rinsing the flow cell...................................................55
8.3.2 Cleaning the lens of an analytical flow cell ...............................56
8.3.3 Cleaning the light guide of a preparative flow cell ........................57
8.4 Replacing the flow cell .........................................................59
9. Transport and storage.................................................................60
9.1 Taking the device out of operation ...............................................60
9.2 Packing the device.............................................................60
9.3 Transporting the device ........................................................60
9.4 Storing the device .............................................................60

Table of contents
BlueShadow Detector 50D Instructions V7659
iv
10. Disposal.............................................................................61
10.1 AVV-Marking Germany .........................................................61
10.2 WEEE registration number ......................................................61
10.3 Eluents and other operating materials ............................................61
11. Technical data........................................................................62
11.1 Main features .................................................................62
11.2 Communication ...............................................................62
11.3 General ......................................................................63
12. Chemical compatibility of wetted materials..............................................64
12.1 General ......................................................................64
12.2 Plastics .......................................................................64
Polyetheretherketone (PEEK) ...........................................64
Polyethylene terephthalate (PET, outdated PETP) .........................64
Polyimide (Vespel®)................................................... 65
Ethylene-tetrafluorethylene copolymer (ETFC, Tefzel®) .....................65
Perfluorethylenpropylen-Copolymer (FEP), Perfluoralkoxy-Polymer (PFA) .....65
Polyoxymethylene (POM, POM-H-TF) ....................................65
Polyphenylene sulfide (PPS) ...........................................65
Polytetrafluorethylene (PTFE, Teflon®) ...................................65
Systec AF™..........................................................65
Polychlortrifluorethylene (PCTFE, Kel-F®)................................. 66
Fluorinated rubber (FKM).............................................. 66
Perfluorinated rubber (FFKM) ..........................................66
12.3 Non-metals ...................................................................66
Diamond-like carbon (DLC) ............................................ 66
Ceramic ............................................................66
Alumina (Al2O3) .....................................................66
Zirconium oxide (ZrO2)................................................66
Sapphire ............................................................66
Ruby................................................................66
Mineral wool.........................................................67
Glass, glass fibre, quartz, quartz glass. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
12.4 Metals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
Stainless steel .......................................................67
Hastelloy®-C .........................................................67
Titanium, titanium alloy (TiA16V4).......................................67

5
BlueShadow Detector 50D Instructions V7659
1. General
1.1 About these instructions
These operating instructions enable the safe and efficient operation of
the device. The user must have carefully read and understood these ope-
rating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see „2 Basic safety instructions“, p. 7). In addition to the
safety and warning instructions in these operating instructions, the local
accident prevention regulations and the national industrial safety regulati-
ons apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library.
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word "Note” and an
information icon.
Note: This is an example.

6General
BlueShadow Detector 50D Instructions V7659
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see „2.2 User quali-
fication“, p. 7)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unaut-
horized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

7Basic safety instructions
BlueShadow Detector 50D Instructions V7659
2. Basic safety instructions
The device has been developed and constructed in such a way that ha-
zards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used for chromatographic applications in
the laboratory.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outside of a laboratory or measurement room. Otherwise,
the manufacturer does not guarantee the functionality and safety of
the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The users are qualified to handle the device if all of the following points
apply:
They have at least a basic knowledge of liquid chromatography.
They have knowledge about the properties of the used solvents and
their health risks.
They are trained for the special tasks and activities in the laboratory
and know the relevant standards and regulations.
Due to their technical training and experience, they can unders-
tand and carry out all the work described in the operating instruc-
tions on the instrument and recognize and avoid possible dangers
independently.
Their ability to react is not impaired by the consumption of drugs,
alcohol or medication.
They have participated in the installation of an instrument or training
by KNAUER or an authorized company.
If users do not meet these qualifications, they must inform their
supervisors.

8Basic safety instructions
BlueShadow Detector 50D Instructions V7659
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and un-
derstood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective afety equipment which is specified in the parti-
cular laboratory

9Basic safety instructions
BlueShadow Detector 50D Instructions V7659
2.5 Working with solvents
2.5.1 General requirements
The user is trained for handling different solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst
(see „12 Chemical compatibility of wetted materials“, p. 66).
Note that organic solvents are toxic above a certain concentration.
For handling hazardous solvents see next section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
Degas solvents before use and observe their purity.
2.5.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see „10 Disposal“, p. 63).
2.5.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch off the system. Only take the device into
operation if the cause of the leak has been resolved (see „8 Mainte-
nance and care“, p. 56).

10 Basic safety instructions
BlueShadow Detector 50D Instructions V7659
2.6 Specific environments
2.6.1 Earthquake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.
2.6.2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2.6.3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.6.4 Wet room
The device must not be operated in wet rooms.
2.7 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer's instructions on
caring for the columns (see www.knauer.net/columncare).
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see „1.4.1 Liability limitation“, p. 6).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting.

11Basic safety instructions
BlueShadow Detector 50D Instructions V7659
2.8 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest.

12
BlueShadow Detector 50D Instructions V7659
3. Product information
3.1 Features
The detector is a measuring system for fast and precise measurement in
the UV range and, with a halogen lamp (optional), also in the visible ran-
ge. The measurement principle is based on the attenuation of a monoch-
romatic light beam passing through liquid.
The self-calibration of the detector guarantees operation without external
settings. Type and combination of the installed lamps as well as the trans-
mission of the corresponding installed flow cell are analyzed and used for
the automatic setting of the integration time, in order to achieve a high
level of sensitivity.
Signal linearity and wavelength accuracy are verified by independent
measurements as part of a self-calibration.
The use of light sources for a specific spectral range is possible
(UV: Deuterium lamp, VIS: Halogen lamp). A tandem operation with
both light sources is also possible, allowing a wavelength range of
190–900nm (UV/VIS) and a wavelength range of 190-750 nm (UV) to
be covered without changing the lamp.
Within a system, it is possible to measure four wavelengths simultane-
ously with the help of the software control.
Recording of a spectrum of 100nm width in less than 100ms.
The beam guidance is designed so that no chromatic aberration
occurs and thus the best possible useful signal isavailable, indepen-
dent of the selected wavelength.
Automatic recording and storage of the device-specific characteristics
that are important for GLP (Good Laboratory Practice) and OQ (Opera-
tion Qualification) or for the device service and device history. These
are operating times, operating parameters, wavelength accuracy and
reference spectra.
Self-running and up-to-date device status diagnostics.
Control with HPLC software possible.
Easy integration of the detector into complex chromatography
systems.
Extremely low noise level and baseline drift.
High data rates for fast chromatography.
Flexible options for use in the entire field of LC applications due to
a comprehensive range of flow cells for the detectors, from nano
HPLC cells with flow rates ≈100nl/min to preparative flow cells with
10l/min.
Self-calibration
Features

13Product information
BlueShadow Detector 50D Instructions V7659
3.2 Scope of delivery
The following items should be included in the scope of delivery:
BlueShadow Detector 50D with test cell
BlueShadow Detector 50D accessory kit
Power cable
Transport protection
Valid documents:
BlueShadow Detector 50D Instructions V7654
Declaration of Conformity
3.3 Views
3.3.1 Front view
The display with the corresponding keypad, the LED light for standby
mode, and the flow cell can be found on the detector‘s front side.
Legend
1 Status LED
2 Display
3 Flow cell with holder
Fig. 1: Front view
123

14 Product information
BlueShadow Detector 50D Instructions V7659
3.3.2 Rear view
The rear of the device contains the mains power connection, power
switch, connections for external devices, technical symbols and serial
number, among other things.
Legend
1 Serial number
2 Fan opening
3 Power switch
4 Power connection
5 Pin header
6 RS-232 port
7 LAN connector
8 Integrator output
Fig. 2: Rear view
3.4 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
High-voltage hazard
Electrostatic discharge hazard, damages to
system, device, or components can occur.
A device marked with CE fulfills the product spe-
cific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally
recognized testing centers (NRTL). The certified
device or system has successfully passed the qual-
ity and security tests.
1 2
3
4
5
6
7
8

15
BlueShadow Detector 50D Instructions V7659
4. Installation and initial startup
Before you determine the operation site, read the chapter “Technical
data” (see chapter 11 on page 64). There you will find all device-speci-
fic information on power supply, ambient conditions and humidity.
Note: The intended use be ensured only if the requirements for ambient
conditions of the operating environment are met.
4.1 Unpacking
Process
1. Place the packaging in such a way that the lettering on the label is
in the correct position.
2. Check the packaging, the device and the accessories for transport
damage.
3. Check the scope of delivery. In the event of incomplete delivery,
contact technical support immediately.
4. When lifting, carrying or moving the device, grab the device only
from below on the sides.
Keep the included packing list for repeat orders.
Keep the original packaging for safe storage or transportation.
4.2 Ambient conditions
4.2.1 Operation site
Observe the following requirements for the operation site so that the
measurement results are not influenced:
Place on a firm, level and straight surface.
Protect against direct sunlight.
Do not expose to air drafts such as air conditioning systems.
Do not set up the to other machines that cause floor vibrations.
Keep from high frequency sources.
Ensure adequate ventilation (see „4.2.3 Space requirements“, p. 16).
Avoid temperature fluctuations (see „4.2.2 Ambient temperature“, p.
15).
4.2.2 Ambient temperature
If the ambient temperature of the device is abruptly changed (e.g. when
it is installed in a cooling chamber), condensation will form inside the
device and may cause damage to the device. Allow the device to accli-
mate for 3 h, before it is connected to the power supply and taken into
operation.
Procedure
Next steps

16 Installation and initial startup
BlueShadow Detector 50D Instructions V7659
4.2.3 Space requirements
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnec-
ted from the power supply.
Ensure adequate ventilation around the device, otherwise it may over-
heat and malfunction:
- Min. 5 cm distance if another device is set on one side.
- Min. 10 cm distance if further devices are set on both sides.
- At least 15 cm to the cooler fan on the rear.
4.3 Power supply
Power supply requirements
Failure-free power supply: For failure-free operation, the electrical
voltage must be free of fluctuations, residual currents, voltage peaks
and electromagnetic interference. The device must receive sufficient
voltage and reserve capacity.
Check voltage: Only connect devices to a power supply whose voltage
corresponds to the permissible voltage of the device.
Power consumption: The nominal power of the connected devices
must not exceed 50 % of the highest connected power capacity, since
higher currents can flow briefly when the device is switched on.
Main connection: The electrical power supply at the operation site
must be connected directly to the nearest main power connection.
Grounding: The connectors for the voltage must be grounded
accordingly.
Power supply cables and plugs
Original parts: For power supply, use the supplied power cable and
plug to meet the specifications which are described in the chapter
“Technical data” (see chapter 11 on page 64). Detachable power
cable cables are not allowed to be replaced with other cable types.
Country-specific plugs: Before switching on the device, check whether
the supplied plug is approved for your country. Overview of the de-
vice- and country-specific plug types from KNAUER:
www.knauer.net/plugs.
Power strips: If several devices are connected to one power strip, al-
ways consider the maximum power consumption of each device.
Access to power supply: Make sure that the power plug on the pow-
er supply (wall socket or power strip) is always accessible, so that the
device can be disconnected easily from the power supply.
Damaged power supply cables and plugs: For safety reasons, dama-
ged or faulty cables and plugs must not be used to connect the device
to the power supply. Replace defective cables and plugs only with
KNAUER accessories.

17Installation and initial startup
BlueShadow Detector 50D Instructions V7659
4.4 Inserting the flow cell
The supply configuration of this detector only includes a test cell (dummy
cell), which does not have connections for solvent. It is used, for example,
to check the lamp intensity, as a dirty flow cell could distort this value.
Before using the device with solvents, the test cell must, therefore, be
removed, and a flow cell must be installed.
The optical path length has been set (optional for special flow cells).
The device has been switched off.
The power supply has been disconnected.
Procedure Process Figure
1. Unscrew the knurled-head
screws 3 of the cover plate
4. Hold the flow cell securely
with your hand during this
procedure.
2. Pull out the slide with the flow
cell 5 towards the front.
3. Remove the flow cell upward.
4. Position the new flow cell on
top of the slide. Keep holding
the flow cell. Because of the
small, drilled hole on the flow
cell (see figure 4), the cell fits
to the detector only when
oriented correctly.
5. Push the slide back into the
detector.
6. Screw the knurled-head
screws back into the cover
plate 4and tighten.
7. Connect the incoming and
outgoing capillaries at the
inlet 1and outlet 2of the
measuring cell.
Fig. 3 Inserting the flow cell
Connect the capillaries.
Prerequisites
Next step
3
4
5
3
1
2

18 Installation and initial startup
BlueShadow Detector 50D Instructions V7659
4.4.1 Changing the optical path length of the prepara-
tive flow cell
At delivery, the optical path length of a preparative flow cell is set to
2mm at the factory. To reduce it to 1.25 or 0.5mm, proceed as follows.
This description applies to preparative flow cells with 1/8“ or 1/4“ connec-
tions.
Procedure Process Figure
1. Remove the threaded ring 1
using a hexagonal spanner.
2. Remove stainless steel cover
2and the PEEK spacer 3.
3. Insert the stainless steel cover
again and carefully tighten
the threaded ring again.
4. The missing spacer causes
the light guide 4to be pus-
hed deeper into the flow cell
(0.75 mm), resulting in a shor-
tened optical path length of
1.25mm. To further shorten
down to 0.5mm, the PEEK
spacer on the other cell side
must also be removed. Fig. 4 Shortening the optical path
length
1
2
3
4