Leica LED1 User manual

1
Leica Microsystems CMS GmbH. Instructions for use, 11934173, Revision 1.0, 2019.05.01
Document Number 57-10022 Revision A
LEICA LED1
LEICA LED3
LEICA LED5
LEICA LED8
External light sources for Leica microscopes
Instructions for use
From Eye to Insight

2
Copyright
Revision 1.0, edited 2019.05.01 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17–37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Copyright
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following docu-
mentation reflect state-of-the-art technology and
knowledge standards. We have compiled the
texts and illustrations as accurately as possible.
Nevertheless, no liability of any kind shall be ac-
cepted for the accuracy of this manual's con-
tents. Still, we are always grateful for comments
and suggestions regarding potential errors within
this documentation.
The information included in this manual may be
changed without prior notice.
As the official manufacturer of Leica branded
light engine products, Lumencor is the respon-
sible owner of this instructions for use 57-10022,
Revision A.
Leica Microsystems CMS GmbH is the distributor
of the Lumencor illumination products detailed
herein, 11934173, Revision 1.0 2019.05.01 is for
Leica internal tracking purposes only.

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Contents
Contents
1. Important Notes about this Manual.............4
2. Function of the Compact Light Source........6
3. Safety Notes.....................................................7
3.1 Regulatory safety notes .................................7
3.1.1 Introduction LED1............................................8
3.1.2 Introduction LED3............................................8
3.1.3 Introduction LED5/LED8..................................8
3.1.4 Precautions and Warnings ...........................9
3.2 Electrical safety.............................................12
3.3 Disposal ..........................................................15
4. Transport, Storage, Unpacking ..................16
5. Notes on Warranty........................................17
6. Overview of the Instrument.........................18
6.1 Intended use ..................................................18
6.2 Identification of the instrument...................18
6.3 Special features of the instrument.............18
6.4 Overview Light Sources ...............................19
6.5 Connecting LED1 ...........................................23
6.6 Connectable light guides (LED3-8) .............23
7. Instrument Setup...........................................24
7.1 Installation......................................................24
7.2 Connecting the LED1.....................................24
7.3 LED3/5/8 – connecting the light guide .......24
7.4 Connecting the Leica POD (optional).........26
7.5 Connecting the power cable.......................26
8. Operation........................................................27
8.1 Operation LED1 ..............................................27
8.2 Operation LED3 ..............................................27
8.3 Operation LED5/8...........................................29
8.3.1 Start Up ...........................................................30
9. Service............................................................31
9.1 Maintenance and cleaning..........................31
9.2 Repairs ............................................................31
10. Troubleshooting ............................................32
11. EC Declaration of Conformity .....................33

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1. Important Notes about this Manual
(1.2)
→page 20
Numbers in parentheses refer to illustrations; for
example, (1.2) refers to Fig. 1, item 2.
Numbers with a pointer arrow (for example →
page 20), point to a certain page of this manual.
WARNING indicates a potentially hazardous
situation with a medium degree of risk that, if
not avoided, could result in death or serious
injury.
CAUTION indicates a potentially hazardous situa-
tion with a low degree of risk that, if not avoided,
may result in minor or moderate injury.
Attention!
In this manual, additional safety notes are in-
dicated with the triangle symbol shown here,
and have a gray background.
Attention! The instrument and its accessories
can be damaged when operated incorrectly.
Text symbols, pictograms and their meanings:
Attention!
This User Manual is an essential component
of the instrument and must be read carefully
before assembly, commissioning or use.
1. Important Notes about this Manual
This User Manual contains important instruc-
tions and information for the operational safety
and maintenance of the instrument and acces-
sories. Therefore, it must be kept in a safe place.
!
WARNING
CAUTION

5
1. Important Notes about this Manual
Warning of hazardous electrical voltage! Danger
of electric shock!
Warning of hot surface.
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operat-
ing lamp. Eye injury may result.
Warning: UV emitted from this product. Avoid eye
and skin exposure to unshielded product.
Warning: IR emitted from this product. Do not
look at operating lamp.
Warning of electromagnetic field
Instructions for disposing of the instrument,
its accessories and consumables.
Earth (Ground) terminal
Explanatory note.
Item not contained in all configurations.
Manufacturer's address with manufacturing date
China RoHS 50 years EFUP
(Environmentally friendly use period)
*
UV
IR
MM/YYYY

6
2. Function of the Compact Light Source
2. Function of the Compact Light Source
The Leica LED1, LED3, LED5 and LED8 compact
light sources, with which this instructions for use
are associated, are intended for biological rou-
tine and research work as well as examinations
in materials science or mineralogy.
Reasonably foreseeable misuse
The following are prohibited:
• Using the light source for any purpose not in
accordance with the Declaration of Conformity
(e.g. use as a medical product in accordance
with EU Directive 93/42/EEC).
• Cleaning the light source in a way other than
specified in the manual.
• Modifying the built-in safety circuits in the in-
strument.
• Allowing unauthorized personnel to open the
instrument.
• Using cables that Leica has not provided or
permitted.
• Using combinations with non-Leica compo-
nents that go beyond the scope of the User
Manual.
• Operating the light source while it is not con-
nected to the stand.
Attention!
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the light source for other purposes than
those for which they are intended or not us-
ing them within the specifications of Leica
Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
Attention!
This instrument is not intended for use in a
patient environment as defined by DIN VDE
0100-710. It is also not intended to be com-
bined with medical devices as defined by
EN 60601-1. If a microscope is electrically
connected to a medical instrument in accor-
dance with EN 60601-1, the requirements de-
fined in EN 60601-1-1 shall apply.

7
3. Safety Notes
3. Safety Notes
3.1 Regulatory safety notes
Leica Microsystems utilizes regulatory model
names for all certified and CE marked products.
The regulatory model names are traceable to
all regulatory documentation, third party reports
and certifications.
“Regulatory Model: LED1/LED3/LED5/LED8 is
used as a representative model for all certified
and CE marked LED1/LED3/LED5/LED8 Products.
Emissions
This equipment has been tested and found to
comply with the limits of EMC directive 2014/30/
EU. These limits are designed to provide rea-
sonable protection against harmful interference
when the equipment is operated in a commercial
environment. This equipment generates, uses,
and can radiate radio frequency energy and, if
not installed and used in accordance with the
instruction manual, may cause harmful interfer-
ence to radio communications.
It also meets the requirements of EN 61326-1,
Electrical equipment for measurement, control
and laboratory use – EMC requirements.
Photobiological Safety
The compact light sources have also been tested
in accordance with EN 62471 / IEC 62471 Photo-
biological safety of lamps and lamp systems and
is classified in risk group 3 (high risk).
Safety Certifications
TÜV SÜD America, CB Certification
(IEC 61010-1:2010)
TÜV SÜD America, NRTLus Certification
(UL 61010-1:2012)
TÜV SÜD America, cNRTL Certification
(CAN/CSA-C22.2 No. 61010-1:2012)
TÜV SÜD America, EN Certification
(EN 61010-1:2010)
CE Marking
Low Voltage Directive (2014/35/EU)
EMC Directive (2014/30/EU)
RoHS Directive (2011/65/EU) plus (2015/863/EU)
REACH Regulation (EC) No. (1907/2006/EC)
In accordance with the REGULATION (EU) No.
1194/2012 OF THE COMMISSION for the imple-
mentation of the Directive 2009/125/EC of the
European Parliament and Council with regard to
the requirements for the environmentally friendly
design of lamps with highly focused light, LED
lamps and associated instruments, the products
described here, Leica LED1, LED3, LED5 and
LED8, are special products because they are not
suitable for general lighting purposes.

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3. Safety Notes
3.1.1 Introduction LED1
The LED1 light source is a compact solid-state
light source for fluorescence microscopy and
other bioanalytical applications. The light source
incorporates efficient thermal management, re-
quiring no fan and providing quiet and vibration-
free operation. This enables direct coupling to
the fluorescence epi-illumination port of a micro-
scope.
The light source provides four distinct color
bands of light output that are controlled using
either manual control knobs on the side panel
or the light source control POD accessory. The
COLOR knob located on the side panel is used for
on/off control, selection of color channels and for
enabling external tablet control. The INTENSITY
knob provides four different levels of output at-
tenuation between 100% and 10% of maximum.
3.1.2 Introduction LED3
The LED3 light sources are designed for labo-
ratory use by bioanalytical researchers and/
or developers of life science instrumentation.
The light sources generate white light output by
combining the outputs of multiple solid state light
sources. The light output on/off status is manu-
ally controlled via a rocker switch located on the
front panel or a foot pedal toggle switch acces-
sory that plugs into the 3.5 mm connector on the
rear panel.
3.1.3 Introduction LED5/LED8
The LED light source consists of 4 (LED5) and 8
(LED8) individually addressable solid-state light
sources with integrated electronic control sys-
tems. The constituent light sources may be LEDs
or luminescent light pipes.
The outputs of the constituent light sources are
refined by bandpass filters and merged into a
common optical train directed to the light output
port on the front panel. The light output port has
a built-in adapter for connection to a liquid light
guide (LLG). An onboard photodiode continuously
monitors the light output and generates refer-
ence signals that can be applied to the constitu-
ent sources in a feedback loop to maintain con-
stant light output over time.
The LED5/8 light sources are controlled by on-
board micro-processors that connect to LAS X
imaging software via a RS-232 (PC) serial in-
terface. The user can enable or disable each
source independently by serial commands, as
well as change the intensity of each source in-
dependently. Alternatively, the light sources may
be turned on and off by TTL inputs from a trigger
device such as a camera or a real-time control-
ler. The only manual control is the master power
switch on the front panel. Optimal internal op-
erating temperature is maintained by negative
pressure air cooling with the air intake at the
front of the light engine and the exhaust fan at
the the rear.

9
3. Safety Notes
3.1.4 Precautions and Warnings
A few simple practices will ensure trouble-free
operation for the life of the light engine.
Safety Instructions:
Please read and follow all safety instructions
provided BEFORE using the light source. Failure
to comply with the safety instructions may result
in fire, electrical shock, or personal injury and
may damage or impair protection provided by
equipment. Please save all safety instructions.
In order to maintain this condition and to ensure
safe operation, the user must follow the instruc-
tions and warnings contained in this User Man-
ual.
Safety Definitions
Warning: Statements identify conditions or prac-
tices that could result in personal injury
Caution: Statements identify conditions or prac-
tices that could result in damage to your equip-
ment.
Safety Items
Warning: ONLY use the power supply provided
by Leica. It is imperative that the DC power sup-
ply has output over-current protection, as the
power input of the light sources are not fused.
The DC power supply must have the AC power
cord connected to a receptacle with a protective
safety (earth) ground terminal
Warning: DO NOT look into the output of the
light engine. The brightness of this light source
is higher than most commercial lighting fixtures
and is intended to couple directly into a micro-
scope or other bioanalytjcal instrument.
Warning: DO NOT turn on the light without the
output end of the light guide safely directed
into an enclosed optical path. DO NOT point the
light output directly onto any flammable or burn-
susceptible material. This includes all animal or
vegetable tissues, plastics, fabrics, paper and
liquids
RISK GROUP 3
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING Possibly hazardous optical
radiation emitted from this product.
Do not look at operating lamp.
Eye injury may result.
AVERTISSEMENT Ce produit émet un
rayonnement optique potentiellement
dangereux. Ne regardez pas la lampe
lorsqu‘elle est allumée. Des lésions
oculaire peuvent en résulter.
Groupe de risque 3
Applicable for LED3/5/8
RISK GROUP 3
WARNING IR emitted from this product.
Do not look at operating lamp. Avoid eye
exposure. Use appropriate shielding or
eye protection.
AVERTISSEMENT Ce produit émet un
rayonnement infrarouge. Ne regardez pas
la lampe lorsqu‘elle est allumée.
Évitez tout exposition aux yeux.
Utilisez un écran ou dispositif de
protection oculaire approprié.
Groupe de risque 3
IR

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3. Safety Notes
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING UV emitted from this product.
Avoid eye and skin exposure to
unshielded product
AVERTISSEMENT Ce produit émet un
rayonnement ultraviolet. Évitez d‘exposer
les yeux et la peau à un produit sans
protection.
Groupe de risque 3
UV
The Leica LED1, LED3, LED5 and LED8 com-
pact light sources generate high-energy light
with invisible UV components and has been
classified in risk group 3 (high risk) in accor-
dance with EN62471-1 while the light guide
is not connected to the microscope/system.
There is a general risk of being dazzled or
blinded by light! Never look directly into the
light guide output of the instrument or into
the output of the light guide connected to the
instrument. Always ensure that the output of
the connected light guide is securely con-
nected to the system to be illuminated before
switching on the compact light source. Fur-
thermore, before switching on the compact
light source, dim the light output to prevent
damage to the connected system. During op-
eration, the light guide must not be removed
from the microscope/system. While connect-
ed to the microscope/system, the risk group 1
(low risk) has been classified. Please see the
instructions on this topic in the microscope
instructions for use.
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operat-
ing lamp. Eye injury may result.
Warning: Infrared (IR) emitted from this product.
Do not look at operating lamp.
Warning: UV emitted from this product. Avoid
eye and skin exposure to unshielded product.
Caution: DO NOT open the unit. There are no
serviceable parts inside and opening the light
engine enclosure will void the manufacturer’s
warranty.
Caution: DO NOT set liquids on the light engine.
Spilled liquids may damage your light engine.
Caution: DO NOT drop the light engine. It con-
tains glass optical components that could be
damaged or misaligned by the shock produced
by a drop onto a hard surface.
DISCLAIMER: Leica shall not be liable for injury to
the user or damage to the product resulting from
the light source being used in a way for which it
was not intended and in complete disregard for
all posted safety precautions and warnings.

11
3. Safety Notes
Attention!
The instruments and accessories described
in this Instructions for use have been tested
for safety and potential hazards.
The responsible Leica representative or the
main plant in Wetzlar must be consulted
whenever the instrument is altered, modified
or used in conjunction with non-Leica com-
ponents that are outside of the scope of this
manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Attention!
If any safety defects or malfunctions of the
compact light sources are identified, the
instrument must be immediately taken out
of operation, disconnected from the power
system at all poles and secured against fur-
ther use. For repair, the compact light source
must be sent to the vendor or an authorized
representative of the vendor.

12
3. Safety Notes
LED3
Supply voltage: 24 V DC, 5.00 A
Power input: 120 W max.
Fuses: Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST120A24-P1M
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output: 24 V DC
5.00 A max.
120 W max.
LED5
Supply voltage: 24 V DC, 9.20 A
Power input: 220 W max.
Fuses: Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST220A24-LC
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output: 24 V DC
9.2 A max.
220 W max.
3.2 Electrical safety
General specifications
For indoor use only.
Frequency: 50/60 Hz
Ambient temperature: 0 to 35°C
Relative humidity: 10 to 80%
non-condensing
Overvoltage category: II
Pollution degree: 2
LED1
Supply voltage: 9 V DC, 4.45 A
Power input: 40 W max.
Fuses: Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST40A09-P1J
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output: 9 V DC
4.45 A max.
40 W max.

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3. Safety Notes
LED8
Supply voltage: 24 V DC, 9.20 A
Power input: 220 W
Fuses: Integrated in external
power supply unit, not
replaceable
Specifications of the external power supply
One of the power supply units listed below
(LPS-certified) must be used:
Mean Well GST220A24-LC
Shared technical data:
Input: 100-240 V AC
50/60 Hz
Output: 24 V DC
9.2 A max.
220 W max.
WARNING
Only use original power cables or alternative
cables with VDE/HAR codes that minimally
fulfill the following conditions: 3 x 0.75 mm2
and 10 A / 250 V.
WARNING
By definition, the main circuit breaker of this
instrument is the connection between the
power cable and instrument port. The user
must ensure unobstructed access to the
main circuit breaker at all times.
CAUTION
Disconnect the power plug before cleaning!
Protect electrical components from moisture.

14
3. Safety Notes
WARNING
The light source must be placed at least
15 cm away from the wall and away from
flammable substances.
Attention!
This light source must not be used at alti-
tudes over 2,000 m above sea level.
WARNING
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not invalidate the grounding function by
using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the instrument, or release
of the protective conductor terminal, can
cause the instrument to become hazardous.
Intentional ground interruption is not permit-
ted!
Attention!
The compact light source are designed for
a voltage range of 100 to 240 VAC, 50/60 Hz.
Within this voltage and frequency range, the
instrument always adapts to the connected
power supply. Operating the instrument with
a power supply voltage outside this range
can destroy the instrument and the connect-
ed components!
Attention!
Do not plug in or unplug data lines and con-
trol circuits unless the instrument has been
shut down; otherwise, the instrument may be
damaged.
Attention!
The instrument is intended exclusively for
operation in dry rooms. Do not use the instru-
ment in rooms with explosion hazard.
Attention!
Do not use this instrument near sources of
high electromagnetic radiation (for example,
unshielded, intentionally operated ultra-high
frequency sources), because these can dis-
rupt proper operation.
We recommend assessing the electromag-
netic environment before operation of this
instrument and then giving corresponding
instructions.

15
3. Safety Notes
WARNING
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps have to be operated in closed hous-
ings and in installed condition.
Never look directly into the beam path (blind-
ing hazard).
3.3 Disposal
Once the product has reached the end of its ser-
vice life, please contact Leica Service or Sales
about disposal.
Please observe and comply with the national and
federal laws and regulations implementing the
WEEE EC directive, for example.
Note!
Like all electronic devices, the microscope,
its accessory components and consumables
must never be disposed of with general
household waste.

16
4. Transport, Storage, Unpacking
Attention!
Transport and storage at -20 °C to +70°C and
max. 80 % humidity (non-condensing).
Attention!
The environmental conditions regarding
transport and storage specified in this In-
structions for use must be complied with. For
safe transport of the external light source,
the original packaging must be used.
If during unpacking of the light source, dam-
age to the instrument is detected, the vendor
or the shipping company contracted by the
vendor must be notified immediately.
Attention!
For unpacking, open the box on its top. It is
then possible to lift the light source out of the
box complete with the transport protection
made of foam. After that, the foam corners
and the plastic bag must be removed from the
instrument. After unpacking, the light source
should acclimatize for about 1 hour before
starting up. This is done to prevent damage
by condensation water that can form due to
temperature differences in the interior of the
instrument.
4. Transport, Storage, Unpacking

17
5. Notes on Warranty
The manufacturer of the instrument warrants
that the instrument is free of material or produc-
tion defects at the time of delivery. Any defects
that have occurred must be reported immediate-
ly, and everything must be done to minimize dam-
age. If such a defect is reported, the vendor of
the instrument is obliged to remedy the defect at
the State Laboratory Services Office's discretion
either by repair or by shipment of an instrument
free of defects. There is no warranty for defects
resulting from natural wear (in particular, in wear
parts) and improper handling.
Attention!
The instrument manufacturer and the ven-
dor of the instrument assume no liability for
damage caused by incorrect operation, neg-
ligence, impermissible use, intervention on
the instrument or the use of spare parts and
components that are not approved. In these
cases, all warranty claims are voided.
No maintenance or repair tasks except for those
listed in this instructions for use may be carried
out on the instrument. Repairs may be performed
only by the vendor of the instrument or the ven-
dor's specially authorized representatives.
5. Notes on Warranty

18
6. Overview of the Instrument
6.1 Intended use
!Attention!
The LED 3,5, and 8 light sources are used to gen-
erate light with a very high intensity and couple
it into the light guide, preferably a liquid light
guide with an active diameter of 3 mm. Any other
use of the instrument is considered noncompli-
ant use.
.
6.2 Identification of the instrument
The nameplate of the instrument is on its rear
side. It includes the necessary information for
identifying the instrument.
6. Overview of the Instrument
In order to maintain this condition and to ensure
safe operation, the user must follow the instruc-
tions and warnings contained in this Instructions
for use.
6.3 Special features of the instrument
For LED3/LED5/LED8, the external light source
has an electrical light output interlock. This en-
sures that the light beam output of the instrument
is closed when the light guide is detached. This
prevents the user from being harmed by the in-
tense light beam generated by the instrument.
Depending on the model of light engine, the light
source can be turned on/off through a variety of
methods. Please confirm the correct light source
operating methods are being used for the instru-
ment:
LED1: The light source can be turned on/off with
the manual light on/off switch on the side panel.
LED3: The light source can be turned on/off with
the manual light on/off switch on the front panel
or in combination with the electronic shutter in-
put on the rear panel.
LED5/8: There is no manual light on/off switch on
the light engine. The light source can be turned
on/off with either the LAS X software or with the
Leica Control POD.
WARNING
The power supply must be set up so that the
power voltage input is not obstructed and the
instrument can quickly be removed from the
power supply if necessary.

19
6. Overview of the Instrument
6.4 Overview Light Sources
Fig. 1 LED1
1 Output adapter
2 On/Off and color switch
3 Intensity switch
4 Light output indicator light
Fig. 2 LED1
1 USB A connection for POD
2 Electronic shutter TTL input
3 Power indicator light
4 Receptacle DC power supply
1
2
3
4
1
2
3
4
Table 1 Output power spectrum LED1

20
6. Overview of the Instrument
Fig. 3 LED3
1 Light indicator
2 Light switch
3 Output adapter
Fig. 4 LED3
1 Power indicator light
2 On/Off switch
3 Foot switch
4 Receptacle DC power supply
5 Electronic shutter TTL input
Table 2 Output power spectrum LED3
1
2
5
3
4
1
2
3