Leica TCS SP8 User manual

10
User Manual
Leica TCS SP8 STED 3X
Leica TCS SP8 STED-ONE
From Eye to Insight

Published by:
Leica Microsystems CMS GmbH
Am Friedensplatz 3
D-68165 Mannheim (Germany)
http://www.leica-microsystems.com
http://www.confocal-microscopy.com
Responsible for contents: Leica Microsystems CMS GmbH
Copyright © Leica Microsystems CMS GmbH.
All rights reserved.

3
Copyright
Copyright
All rights to this document are held by Leica Microsystems CMS GmbH. Adaptation,
translation and reproduction of text or illustrations (in whole or in part) by print, photocopy,
microfilm or other method (including electronic systems) is not allowed without express
written permission from Leica Microsystems CMS GmbH.
Programs such as LAS and LAS X are protected by copyright laws. All rights reserved.
Reproduction, adaptation or translation of these programs is prohibited without prior written
permission from Leica Microsystems CMS GmbH.
AOBS® is a registered trademark of Leica Microsystems CMS GmbH.
This User Manual specifies names of products or services that are trademarks or registered
trademarks of the respective trademark owners. Rather than including a trademark (TM or
®) symbol at every occurrence of a trademarked name, we state that we are using the
names only in an editorial fashion, and to the benefit of the trademark owner, with no
intention of infringement.
Made in Germany.
© Copyright Leica Microsystems CMS GmbH.
All rights reserved.

4
Copyright

5
Contents
Contents
1 About this User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
2 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
3 Liability and Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
3.1 Important Information for Operators and Users. . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
4 Meaning of the warning messages in the manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
5 General safety notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
5.1 Commissioning and use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
5.2 Electrical safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
5.3 Safety Devices and Safety Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
5.4 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
5.5 Contact with liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
5.6 Modifications to the system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
5.7 Malfunction of the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
6 Additional notes on handling the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
6.1 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
6.2 Using the software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
6.3 Protecting the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
6.3.1 Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
7 System Overview and Properties. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
7.1 System variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
7.1.1 System with Inverted Microscope and Flexible Supply Unit . . . . . . . . . . . . . . .25
7.1.2 System with Inverted Microscope and Compact Supply Unit . . . . . . . . . . . . . .26
7.1.3 System with Upright Microscope and Flexible Supply Unit . . . . . . . . . . . . . . . .27
7.1.4 System with Upright Microscope and Compact Supply Unit . . . . . . . . . . . . . . .28
7.2 Controls on the Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
7.2.1 Main Switch Board on the Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . .29
7.2.2 Control Panel Field on the Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . .29
7.3 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
7.3.1 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
7.3.2 Weight of the supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
7.3.3 Electrical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
7.4 "Electromagnetic Compatibility". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
7.5 Serial Number. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31

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Contents
8 Ambient Conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
8.1 General Requirements Regarding Ambient Conditions. . . . . . . . . . . . . . . . . . . . . . .33
8.2 Vibrations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
8.3 Room Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
8.4 Electrical Connection Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
8.4.1 System with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
8.4.2 System with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
8.4.3 External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
8.5 Load capacity of the multiple socket outlet on the flexible supply unit . . . . . . . . .37
8.6 Waste Heat and Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
8.6.1 System with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
8.6.2 System with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
8.6.3 External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
9 Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
9.1 Laser Classes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
9.2 Overview of Usable Lasers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
9.2.1 VIS lasers for STED System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
9.2.2 IR Lasers for STED System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
9.2.2.1 Picosecond Lasers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
9.2.2.2 Femtosecond laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
9.2.3 Depletion Laser for TCS SP8 STED System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
10 Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
10.1 Laser Safety Chamber for Multi-Photon Applications. . . . . . . . . . . . . . . . . . . . . . . .43
10.2 Main Circuit Breaker for Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . .43
10.2.1 Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
10.2.2 Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
10.3 Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
10.3.1 Master Key Switch on the Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . .44
10.3.2 Master Key Switch on the Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . .44
10.3.3 Key Switch for the White Light Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
10.3.4 Key Switches for Other External Lasers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
10.4 Emission Warning Indicators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
10.4.1 Emission Warning Indicator on the White Light Laser . . . . . . . . . . . . . . . . . . . .47
10.4.2 Emission Warning Indicator on Other External Lasers . . . . . . . . . . . . . . . . . . . .47
10.4.3 Malfunction of Emission Warning Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
10.5 Interlock Connectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
10.5.1 Interlock Connector on the Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
10.5.2 Interlock Connector on the Laser Safety Chamber . . . . . . . . . . . . . . . . . . . . . . .49

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Contents
10.5.3 Manual Laser Interlock Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50
10.5.4 Interlock Connector on the White Light Laser . . . . . . . . . . . . . . . . . . . . . . . . . . .50
10.5.5 Interlock Connector on Other External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . .51
10.5.6 Interlock Connector on the Scan Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
10.6 Safety Switches on the Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
10.7 Warning Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
10.8 Special Laser Safety Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
10.8.1 Laser Protection Tube and Laser Protection Shield . . . . . . . . . . . . . . . . . . . . . .54
10.8.2 Safety beam guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
11 Safety Labels on the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
11.1 Safety labels on the Laser Safety Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
11.2 Inverted Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
11.2.1 Inverted Microscope with DLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
11.3 Upright Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
11.4 Scan Head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
11.5 STED Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
11.6 Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
11.7 Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66
11.8 Beam Coupling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
11.9 Cover for Replacement Flange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68
11.10 HyD RLD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69
11.11 White Light Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69
12 Switching the System On/Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
12.1 System with Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
12.1.1 Switch-on Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
12.1.2 Switch-off Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74
12.2 System with Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
12.2.1 Switch-on Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
12.2.2 Switch-off Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78
12.3 HyD reflected light detectors (HyD RLDs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
13 LAS X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
13.1 Starting LAS X. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
13.2 Structure of the Graphical User Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
13.3 Activating/Deactivating the STED in LAS X. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
13.4 LAS X Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
13.4.1 Accessing Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86

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Contents
13.4.2 Selecting the language for Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
13.4.3 Using Online Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
13.4.4 Searching in Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
14 Changing the Specimen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89
14.1 Changing the Specimen on an Upright Microscope . . . . . . . . . . . . . . . . . . . . . . . . .89
14.2 Changing the Specimen on an Inverted Microscope . . . . . . . . . . . . . . . . . . . . . . . .89
15 Changing the Objective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
16 Lambda/4 Plate Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
16.1 Changing the Filter Cube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
17 Care and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
17.1 Cleaning surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
17.2 Cleaning optical components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
17.2.1 Cleaning the front lens of immersion objectives. . . . . . . . . . . . . . . . . . . . . . . . . .96
17.3 Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
18 Repairs and Service Work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
19 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
19.1 Having Coolant Replaced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
19.2 Cooling systems on external lasers and other peripheral devices . . . . . . . . . . . . .99
19.3 Refilling Coolant in the External Coolant Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . .100
20 Disassembly and Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101
21 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103
22 Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
23 Appendix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
23.1 Patents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
23.2 Safety Data Sheets from Third-Party Manufacturers . . . . . . . . . . . . . . . . . . . . . . .107
23.3 Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113
23.4 People's Republic of China. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115

9
About this User Manual
1 About this User Manual
Prior to commissioning the system, carefully read through this User Manual and be
absolutely certain to follow the safety notes contained in it. So that you can operate the
system safely and react quickly and correctly in the event of an emergency, you must
familiarize yourself with the safety devices before using it for the first time. In this case, read
Chapter "Safety Features" in this manual. Keep this User Manual and the included manuals
for the microscope and other components in a safe place easily accessible for all users.
This Manual gives you important information about safe handling of the system. All
information is intended for the safety of users and trouble-free operation of the system.
Unless the information pertains specifically to certain system variants, the instructions
always apply to the basic system described here.
Figures are for illustration purposes. The system you purchased can deviate from the
illustrations without Leica Microsystems CMS GmbH explicitly specifying such.
This User Manual provides you with important information for using the system, the
necessary ambient conditions and the usable lasers. It explains system startup. The system
is assembled and disassembled by service technicians that have been authorized by Leica
Microsystems CMS GmbH. This is why unpacking, assembly and installation of the system
are not described in this manual. You can find an overview of the system and specifications
in the Chapter "System Overview and Properties". For information about special
configurations, such as optional lasers or specific objectives, refer to the respective
included manual.
This User Manual does not contain any information about basic optical principles or the
operating principle of microscopes, confocal systems and the like. If you are interested in
these topics or certain applications from the area of optics and confocal microscopy, you
can read more about them at the Leica Microsystems CMS GmbH knowledge portal: http://
www.leica-microsystems.com/science-lab/
The system is delivered with the latest version of the licensed LAS X software. In order to
keep information up-to-date, a description of software functions has been intentionally
omitted from this user manual. You can find up-to-date explanations and instructions for
corresponding software functions in the LAS X Online Help. Read Chapter "LAS X" in this
User Manual in order to familiarize yourself with the design and basic operation of the
software. Additional information about specific functions can be found in the online help.
The instructions contained in this documentation reflect state-of-the-art technology and
knowledge standards at the time of publication. Leica Microsystems CMS GmbH reserves
the right to revise this documentation and/or to further develop and improve the products
described in this document at any time without prior notice or any other obligation.
If you have any suggestions or improvements for this User Manual, please contact the Leica
branch office in your country.

10
About this User Manual

11
Intended Use
2 Intended Use
This system is intended for use in a lab. The system was designed for confocal and STED
scanning (laser scanning images) of fluorescence-labeled living and fixed specimens as
well as for quantitative measurements in the area of life science.
Applications of in-vitro diagnostics in accordance with the European guideline 98/79/EC are
excluded from proper intended use.
This system must not be used together with life-support systems such as those found in
intensive-care wards.
The owner/operator and user of this product are responsible for proper and safe operation
and safe maintenance of the system and for following all applicable safety regulations. The
owner/operator and user are fully liable for all consequences resulting from the use of the
system for any purposes other than those listed in the User Manual or the online help.
The manufacturer assumes no liability for damage caused by, or any risks arising from, use
of the microscopes for purposes other than those for which they are intended, or not using
the microscopes within the specifications of Leica Microsystems CMS GmbH. In such
cases, the Declaration of Conformity shall be invalid.

12
Intended Use

13
Liability and Warranty
3 Liability and Warranty
Leica Microsystems CMS GmbH shall not be liable for damages resulting from failure to
observe the information in this User Manual. The information here does not in any way
modify the warranty and liability clauses contained in the general terms and conditions of
Leica Microsystems CMS GmbH.
Repairs and servicing may be performed only by service technicians authorized by Leica
Microsystems CMS GmbH. Opening or working on the system in any way shall void any and
all warranty claims.
The manufacturer assumes no liability for damage caused by, or any risks arising from, use
of the microscopes for purposes other than those for which they are intended, or not using
the microscopes within the specifications of Leica Microsystems CMS GmbH. In such
cases, the Declaration of Conformity shall be invalid.
Leica Microsystems CMS GmbH shall not be liable for any damage caused by incorrect
storage, improper transport or an unsuitable installation location.
Figures are for illustration purposes. The system you purchased can deviate from the
illustrations without Leica Microsystems CMS GmbH explicitly specifying such.
Leica Microsystems CMS GmbH shall not be liable for any injury or property damage caused
by untrained or unauthorized persons.
3.1 Important Information for Operators and Users
• The owner/operator is required to designate a Laser Safety Officer or a Laser Protection
Advisor according to the applicable legal requirements in each country.
• The owner/operator and user of this product are responsible for proper and safe
operation and safe maintenance of the system and for following all applicable safety
regulations.
• The owner/operator and user are fully liable for all consequences resulting from the use
of the system for any purposes other than those listed in the User Manual or the online
help.
• The owner/operator and user are obligated to perform and monitor suitable safety
measures (according to national regulations).
• The owner/operator and user are responsible for observing the laser safety regulations
according to applicable country-specific regulations.
• The owner/operator and user must ensure that this laser product is commissioned and
operated only by persons who have been trained in the use of the system and the
potential dangers of laser radiation.
• The owner/operator and user are fully liable for all consequences resulting from the use
of the system if it is opened, improperly serviced or repaired by persons other than
authorized Leica service representatives.

14
Liability and Warranty

15
Meaning of the warning messages in the manual
4 Meaning of the warning messages in the manual
WARNING Electric shock
This warns you of hazardous electrical voltage. Following the
instructions is mandatory, since otherwise there is a risk of severe or
fatal injury.
WARNING Severe injuries from ...
This note warns you of hazards that can cause severe or fatal injuries.
WARNING Permanent eye and skin damage from laser radiation
This note warns you of eye and skin damage that can occur when using
lasers if safety precautions are not taken.
WARNING Risk of injuries due to harmful or irritating substances
This note warns you of substances that pose a health hazard.
WARNING Risk of injuries due to biological substances
This note warns you of biological substances that pose a health hazard.
WARNING Risk of burns on hot surfaces
This note warns you of hot surfaces that can cause burns.

CAUTION Injuries from…
This note warns you of minor to moderate injuries that can be prevented
by following instructions.
NOTICE Risk of damage to the system
This note describes possible material damage that can occur in case of
misuse.
NOTICE Loss of data
D A T A
This note warns you of the potential for losing data.
Observe user manual
This mandatory sign indicates that an additional user manual must be
followed.
Wear laser safety glasses
This mandatory sign indicates that laser safety glasses must be worn to
prevent eye injuries.
Additional note
This note serves to emphasize important instructions for handling the
product or contains special instructions about a certain topic.
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Meaning of the warning messages in the manual

17
General Safety Notes Revision
5 General safety notes
You have to follow the instructions listed below to work with the instrument safely and
without disturbance. Leica Microsystems CMS GmbH shall not be liable for personal injuries
or property damage resulting from noncompliance.
Not every possible hazardous situation can be foreseen; therefore, perform all actions on
the system carefully and while exercising common sense.
5.1 Commissioning and use
• The system is delivered in secure transport packaging. Only Leica service technicians or
people authorized by Leica Microsystems CMS GmbH may open the transport packaging
and unpack and assemble the system.
• Before handling the system, receive instruction on the dangers of laser radiation and on
suitable laser safety precautions, such as wearing suitable laser safety goggles before
beginning to handle the system (laser safety officer). This applies to all persons present
in the room where the system is set up and operated.
• Read the instructions included carefully and follow the instructions it contains.
• Do not introduce any reflective objects or mirrors into the laser beam path or specimen
area when working on a laser.
• Do not introduce any flammable objects into the beam path or specimen area when
working on a laser.
• Do not place any flammable or combustible objects on or near the system and do not put
it near hot surfaces.
• Keep your hands away from the specimen area during start up and operation. Otherwise,
there is a risk of crushing/injury from rotating objectives and the motorized specimen
stage.
• If the specimen carrier breaks, there is a risk of cutting injury and, depending on the kind
of specimen, risk of contamination. Take suitable actions to remove fragments and
prevent cutting injuries.
• Set up the workplace (for example, chair and monitor) on the system according to your
requirements. Observe the national regulations for occupational safety.
• Clean the system thoroughly first before a service technician carries out maintenance or
before each relocation. If necessary, the surfaces must be disinfected appropriately to
eliminate potential contaminations and, therefore, prevent hazardous substances and
infectious agents from carrying over. The same also applies to the removal of
components. This applies in particular to systems that are located in biomedical
research labs.
• Do not deviate from the operating and maintenance instructions provided herein.
• Do not rearrange the components. If you have any further questions regarding the
placement and arrangement of components, please directly contact the Leica branch
office in your country.
• Ensure that the equipment for cooling components (including ventilation slots, apertures
or fans) are not adjusted or covered because this can cause the components to overheat

18
General Safety Notes Revision
and destroy them.
5.2 Electrical safety
• Only operate the system with and connect it to grounded sockets. Operation without
grounded sockets is not allowed. Otherwise, risks such as electrical shocks or property
damage can result.
• Make sure that the supply voltage at the system remains in an approved tolerance range
(100 V~ - 240 V~ ±10%).
• Only connect the system to a power supply with a ground protection conductor and do
not use extension cords without ground protection conductors. Using extension cords
without ground protection conductors is not allowed.
• Do not interrupt the ground protection conductor within or outside of the system and do
not disconnect it. Otherwise, risks such as electric shocks can result.
• Under no circumstances may you open housing parts since the underlying components
are energized and can harm you.
• Before any cleaning or servicing, de-energize the entire system. To do so, use the power
switches of all components and disconnect all power cables from the power supply.
• Only use the power cables included or provided by your local Leica service technicians
for connecting individual peripheral devices to the power supply.
• Fuses inside the system may be replaced only by authorized Leica service employees. If
you have any further questions, please directly contact the Leica branch office in your
country.
5.3 Safety Devices and Safety Labels
• Carefully read the Chapter "Safety Features" of this User Manual before initial use to
familiarize yourself with the safety devices of the system. This is the only way to be able
to operate the system safely and react correctly and quickly in emergencies.
• Keep all safety and laser protection equipment in a ready-to-operate condition.
• Never remove, change, deactivate or damage the safety and laser protection equipment
on the system. Otherwise, serious injuries or damage to property may result.
• Do not remove any safety labels on the system.
• Attach missing or damaged safety labels immediately and at the described location.
Observe Chapter "Safety Labels on the System".
5.4 Laser Safety
• The system is a Class 3B/IIIb (VIS and UV lasers) or a Class 4/IV (IR lasers) laser product.
• Adhere to all necessary safety measures applicable for each laser class.
• Do not switch on the system if you detect damage to the fiber optic cables. Laser
radiation can escape and lead to irreparable eye and skin injuries.

19
General Safety Notes Revision
• Do not introduce any reflective objects or mirrors into the laser beam path or specimen
area when working on a laser.
• Never expose your eyes or skin to direct or indirect laser radiation (reflections). The
radiation can cause irreparable eye and skin injuries.
• Do not look into the eyepieces when scanning or switching the beam path in the
microscope.
• During the scanning operation, the laser radiation is accessible in the microscope's
specimen area without obstruction after coming out of the objective. Always maintain a
nominal ocular hazard distance of at least 20 cm (8") between your eyes and the opening
of the objective.
Figure 1: Specimen area of upright and inverted microscope
• Only use S1 and S28 Leica microscope condensers.
• Do not use an S70 microscope condenser. Its large working distance and lower
numerical aperture could pose a hazard due to laser radiation.
• Never change any samples, objectives, filter cubes, beam splitters, condensers or other
components during a scanning operation.
• If there is no lamp housing or mirror housing connected to the microscope, attach the
corresponding cover to the replacement flange.
• Close all unoccupied positions in the objective turret using the supplied caps.
Special features for MP systems:
• When using a MP system, you must wear suitable laser safety goggles. Appropriate
laser safety goggles for IR laser radiation are provided with the system when delivered.
These laser safety goggles do not provide any protection against visible laser radiation
(visible spectrum)! During the scanning operation, all persons present in the room must
wear such laser safety goggles. If you need additional safety goggles, please contact the
Leica branch office in your country directly.
• Do not use any dry objectives with a numerical aperture (NA) greater than 0.85. This does
not apply to immersion objectives (oil, water).
5.5 Contact with liquids
• Do not expose the system to rain or humidity. Otherwise, risks such as electrical shocks

20
General Safety Notes Revision
or fire hazards can result.
• Make sure that no liquid enters the system housing or comes into contact with any
electrical components.
• Avoid condensation.
• The system must be completely dry before connecting it to the power supply or turning
it on.
• Do not operate the system if coolant is leaking or has leaked.
5.6 Modifications to the system
• The system is installed by service technicians from Leica Microsystems CMS GmbH. Do
not change the position of the system components.
• Always set up and operate the supply unit in an upright position.
• Under no circumstances may you open housing parts since the underlying components
are energized and can harm you.
• Never disconnect a fiber optic cable.
• Make sure that the cables, fiber optic cables and cooling liquid hoses are not stretched,
pinched or rolled up tightly or damaged in any other way.
• Do not connect any external equipment or other components. If you have questions,
please directly contact the Leica branch office in your country.
5.7 Malfunction of the system
You must immediately disconnect the system from the power supply if any of the following
occur:
• The emission warning indicator is not lit after being switched on using the key switch.
• The emission warning indicator is re-lit after being switched off using the key switch.
• Scanning of the specimen is not activated after being switched on properly (laser
radiation in the specimen area).
If any of these occur, immediately notify the Leica branch office in your country or your local
contact person.