LW Scientific Combo V24T User manual

Combo V24T Centrifuge
Instruction Manual
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
Before Operating
This symbol refers to hazards that may be encountered when using this product.
CAUTION means that damage to product or environment could occur
WARNING means that injury or contamination could occur
LW Scientific instruments have a one (1) year limited warranty. This warranty is not valid on normal wear and tear, cosmetic
damages caused by chemicals, solvents, and/or cleaning solutions, as well as acts of God.
Please register your product online at: www.LWScientific.com/warranty_form.
Important: Warranty information must be completed within 30 days of purchase.
Remove the centrifuge from the shipping container and inspect for any possible shipping damage. If the centrifuge
appears to be damaged, please contact your distributor immediately.
Open the lid by pressing on the front center of the lid until it clicks (to close, press down again until it clicks).
The Combo V24T normally ships with the 12-place test tube rotor installed. Ensure the black thumbscrew is tight before
operating as seen on page 2.
WARNING: Ensure the rotor is securely fixed to the rotor shaft. Failure to properly secure rotor could lead to personal
injury or damage to the centrifuge.
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2
B
A
Lid
Main Power Switch
Display Power
Start / Stop
Rotor Select
Speed
Time
Short Spin
Select
Descrease Value
Increase Value
Start
Stop
time
speed
Short
Spin
Rotor
select

WARNING: Ensure the rotor is securely fixed to the rotor shaft. Failure to properly secure rotor could lead to personal
injury or damage to the centrifuge.
WARNING: Be sure the rotor thumbscrew is tightened before each centrifugation cycle. Failure to properly tighten
thumbscrew may lead to a bent or broken thumbscrew and damage to the tube shields, rotor, and rotor chamber.
Place the centrifuge on a sturdy, level surface. Verify that there are no loose objects or packing material in the tube
chamber. DO NOT PLUG UNIT IN.
Select a rotor and slide it down over the motor shaft, ensuring that the slot in the bottom of the rotor lines up with cross
pin on the motor shaft. The shaft should be lubricated with a fine layer of common grease every few months for easy
rotor changes.
NOTE: Make sure there is no grease on the top of the surface of the rotor to ensure the thumbscrew stays secure.
Test Tube and Microtube Rotors: After seating the rotor properly on the motor shaft cross pin, tighten the black thumb-
screw down on the rotor. Do not over-tighten.
Verify that the main power switch is in the “OFF” position and plug the AC power cord into the appropriate approved and
properly grounded AC outlet. Next, turn the main power switch on, then press the front panel POWER button. The display
should illuminate.
DO NOT INSERT TUBES AT THIS TIME: Ensure that the Rotor Select is in the correct position: LOW SPEED for test tube rotors
(1,000-5,000 rpms) or HIGH SPEED for microhematocrit and microtube rotors (12,000 rpms).
To change speeds, press and hold the ROTOR SELECT button for three seconds.
SPEED and TIME can only be adjusted when the lid is closed and locked.
To set the speed, press the SPEED button once, then use the UP/DOWN arrows to select 1,000 rpm, for an initial test run. Press
the SPEED button again to lock the speed.
To set the time, press the TIME button once to flash seconds, and use left arrow to flash minutes. Use the UP/DOWN arrows
to choose 1:00 minute for the initial test run. Press the TIME button again to lock the time.
Start the unit by pressing the START/STOP button. The unit should come up to speed with a smooth sound and little or no
vibration. If there is excessive vibration or noise, shut off the unit immediately, check the troubleshooting tips, and contact
LW Scientific if not resolved.
Spin only balanced loads. Make sure that tubes are placed opposite each other. Proper sample balancing will improve
sample separation and will extend the life of the centrifuge. Out of balance loads may damage the centrifuge.
Support your tubes: Tubes must always be supported from the bottom and never hanging by their caps. Use the large tube
sleeves for larger tubes and the small sleeve inserts for smaller tubes. Rubber tube cushions are also available from LW
Scientific for supporting short tubes in the large tube sleeves.
Ready for operation: Load your tubes and set time and speed. When spinning the 24 -place microhematocrit rotor, keep
the speed set at 12,000rpm for rapid and complete separations in 3 minutes.
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4
5
6
7
8
9
Selecting and Changing Rotors
A
B
C
D
NOTE: Please ensure that the black rubber rotor gasket is
positioned around the rim of the rotor such that the capil-
lary tubes are pushing into the rubber while spinning. The
rubber gasket is not affixed to the rotor to allow for clean-
ing and re-positioning of the gasket to prolong gasket
life.
10
High speed
Pcv/microtube rotors
Low Speed
Test Tube Rotors
Rotor
select
WARNING: Spinning the 12-place test tube rotor at high speeds (above 5,000 rpm) can cause tube breakage and centri-
fuge damage and cause an over-speed error.
Microhematocrit Rotor: Place the metal cover plate onto the rotor by sliding it down over the center shaft.
WARNING: The rotor cover plate is not threaded and will not hold the rotor in place during operation.
Next, tighten the black thumbscrew down onto the rotor. Do not over-tighten.

Reading Microhematocrits
After the rotor has stopped, remove the cover plate by unscrewing the black thumbscrew and lifting the cover plate
straight up.
Place the reader disc over the hematocrit tubes, and adjust the disc until the 0% line is lined up with the bottom of the fluid
in the tube to be read.
While keeping the 0% line positioned at the bottom of the fluid, turn the disc until the 100% line aligns with the top of the
fluid.
Read the separation line in the middle for your microhematocrit results.
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2
3
4
If you purchased a microhematocrit rotor, it includes a Reader Disc, which allows the tubes to be read directly from the rotor.
Care, Maintenance, Trouble-Shooting
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9
Use only quality test tubes. Lower-quality plastic or glass tubes may fracture and release their contents into the tube cham-
ber.
Never force a tube into the tube shields. Some plastic conical test tubes are too large and should not be used.
Never hang a tube by its cap. Always make sure the tubes are supported by the bottom of the shield, or with a rubber cush-
ion.
Use only 75mm capillary tubes with the 24-place microhematocrit rotor.
Clean the inside of the centrifuge regularly. Always unplug electrical power before cleaning. Whenever there is a tube
breakage or a spill, it is recommended that the inside of the centrifuge be cleaned, and if possible, disinfected (do not
immerse the electrical components in any liquid).
The Combo V24T motor shaft has been greased prior to shipping. It is recommended to put a light coating of grease onto
the motor shaft every few months to allow for easy rotor changes. Any type of household grease will work, but a white
lithium grease is best because it makes the metal surfaces friction-free and protects against rust. DO NOT use oils or spray
lubricants (eg. WD-40) which will not coat the surfaces well.
If you have difficulty removing the rotors, you may have light oxidation on the motor shaft. To correct this we recommend
a clean-polish-clean procedure. First clean the motor shaft with alcohol, then polish it with 400-grit (or finer) sand paper or
steel wool, then clean the shaft again. Then apply a thin coating of grease to the motor shaft. If the problem persists, it is
recommended to clean the shaft with alcohol and reapply grease every few weeks.
NOTE: Make sure there is no grease on the top of the surface of the rotor to ensure the thumbscrew stays secure.
Display comes on - Will not spin: Check to make certain the lid safety switch (near the back right hinge) is engaged. With
the power on and the timer set, depress the button with your finger. If the rotor spins, turn the safety switch button count-
er-clockwise to raise the height and allow it to contact the lid when it is closed.
No light - Not spinning: Confirm that your power source is working. Check the 6.3 amp fuse and replace if the filament is
broken. Speed slows down or stops...the brushes may need to be replaced on the motor after 1000 +/- hours of use if speeds
have decreased. Contact LW Scientific to purchase new brushes (part #CMP-BRSH-PR77).
Because of the safety issues with high g-forces in a centrifuge, it is recommended that rotors be inspected every 6 months
for corrosion and fatigue. If there is any indication of wear, the rotor should be removed from service. Contact LW Scientific
for return instructions, so the rotor can be evaluated by an LW Scientific technician for repair or replacement. It is also
recommended that after 2 years of service rotors and tube shields* be returned to LW Scientific for inspection. Following
these procedures will ensure safety of lab personnel as well as extend the life of the centrifuge.
NOTE: Plastic tube shields should be replaced at least once a year. Tube shields should be inspected once a month for wear
or damage. Tubes that are being used in high volume applications should be replaced every three months or sooner.
WARNING: Failure to secure rotor or rotor lid could lead to personal injury or damage to the centrifuge.
CAUTION: If corrosion, scratches, or other abnormalities are found on rotor or tube shields, discontinue use and
contact our service center.
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P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
MKT-7.5.3-L-319 | Rev 0
Specifications
Max. Speed (HIGH):
Max. Speed (LOW):
Max. Volume:
Input Voltage:
Fuse:
Height:
Width:
Depth:
Weight:
12,000 RPM (+/- 600)
5,000 RPM (+/- 200)
12 3ml to 15ml test tubes
24 75mm capillary tubes
Optional 24 2ml microtubes
110v / 220v auto-switching
6.3 amp / 250 volt
11 inches (277mm)
14 inches (355mm)
17 inches (432mm)
31.3 lbs (14.2kg)
Recommended Fluid Speeds & Times:
Blood (Serum) 5,000 rpms 5 minutes
Urine 1,700 rpms 5 minutes
Fecals 1,500 rpms 6 minutes
Microhematocrits 12,000 rpms 3 minutes
RPM
500
1,000
1,500
1,700
2,000
2,500
3,000
3,500
4,000
4,500
5,000
12,000
12,000
G-Force (RCF)
31
123
277
355
492
769
1,107
1,507
1,968
2,490
3,075
12,900
14,972
Test Tube Rotor
(11cm radius)
Microtube Rotor
Microhematocrit Rotor