Miltenyi Biotec autoMACS Pro Separator Manual

Load samples
Touch to start up
Use the touch screen to program your cell
separation procedures.
Scan reagents
with the
2D barcode
reader
A Ori
B Neg
C Pos
Chill 15 Rack
A Ori
B Neg
C Pos
Chill 50 Rack
A Ori
B Neg
Chill 5 Rack
C Pos
Consult the User Manual before operating this instrument for the rst time. Unless otherwise specically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, MACS, and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its aliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its aliates. All rights reserved.
autoMACS® Pro Separator
Short instructions
140-002-015.07

Consult the User Manual before operating this instrument for the rst time. Unless otherwise specically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, MACS, and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its aliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its aliates. All rights reserved.
Load
Template
Save
Template
Delete
Selected
Run
2345 6
Wash
Now
ii i
Stop
Wash
Now
Load
Template Save
Template
Delete
Selected
Run
Reagent
Separation
Status
Log list
Option
Labeling Volume Separation
1
Wash
Reagent
Separation
R1
R2
R3
R4
Status
Stop
Log list
Option
Enter
Reagent
Load
Template
Save
Template
Delete
Selected
Read
Reagent
Info
Enter reagents
AGo to the Reagent menu and highlight a reagent rack position.
BPress the Read Reagent button.
Present a reagent vial in front of the blinking 2D code reader.
CEnter up to four reagents.
DSave as a template if desired.
Define the separation procedure
EGo to the Separation menu.
FHighlight one or more samples.
GSelect the desired Labeling reagent.
HTouch the Volume submenu to enter the sample volume.
ISelect a Separation and a Wash program.
JPlace reagent vials and sample tubes on the respective racks and press Run.
autoMACS® Pro Separator
Short instructions – sample labeling and separation

Consult the User Manual before operating this instrument for the rst time. Unless otherwise specically indicated, Miltenyi Biotec products and services are for research use only and not for
therapeutic or diagnostic use. autoMACS, MACS, and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its aliates in various countries worldwide.
Copyright © 2017 Miltenyi Biotec GmbH and/or its aliates. All rights reserved.
Replace Fluid bottles
1Take out an empty bottle and unscrew bottle
closure counter-clockwise but do not remove it.
Do not disconnect the color-coded tubing.
2Place a fresh bottle into the holder, open it
and fasten the bottle closure to the new bottle.
Note the color-coding.
Column exchange
1Open the front door.
2Ensure that the fluid bottles are filled with
solutions.
3Go to Option > Special > Col_ex.
4Press Run. Wait until the instrument prompts
you to exchange columns.
5Pull out the column using both hands.
6Unscrew first bottom and then top column
connector counter-clockwise.
7Insert a fresh column and fasten it to the column
connectors.
8Press the column back into its slot until you hear
a click. Repeat the whole process with column 2.
9Press Done.
Priming
Prime the instrument after it is switched on:
1Go to the Separation menu and press
Wash Now.
2Select Rinse and press Run.
Cleaning
Before shutting down, clean the instrument:
1Press the shutdown button at the upper
right hand corner of the screen.
2Select Yes.
3Upon completion of the Sleep program, switch
off the Instrument using the main power switch
on the lower right side of the instrument.
autoMACS® Pro Separator
Short instructions – maintenance
140-002-015.07

Positive
selection
Labeling of
target cells
Frequency
of target
cells?
>5 to 10%
<5 to 10%
Normal to high
Low
Normal to high
Low
Level of
antigen
expression?
Level of
antigen
expression?
POSSEL
Positive selection, standard mode
POSSEL_S
Positive selection, sensitive mode
POSSELD2
Double-positive selection
POSSELD
Double-positive selection, low elution volume
POSSELDS
Double-positive selection, sensitive mode
POSSELWB
Double-positive selection for whole blood
and bone marrow applications
POSSELBM
Positive selection for bone marrow applications
Recovery is highest priority
Purity is highest priority
Depletion
Labeling of
unwanted cells
Depletion
using MACS®
MicroBeads
Depletion using
MACS® Cell
Isolation Kit
DEPL05 or DEPL025
Special programs for very sensitive depletion
DEPLETES
Depletion, sensitive mode
DEPLETE
Depletion
Low
Normal to high
Level of
antigen
expression?
Recovery is highest priority
Purity is highest priority
Recovery is highest priority
autoMACS® Pro Separator
Short instructions – separation strategy

Consult the User Manual before operating this instrument for the rst time. Unless otherwise specically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, MACS, and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its aliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its aliates. All rights reserved.
Cell Separation Reagent Strategy No. of
reagents
Dilution volume Autolabeling
Minimal volume*Minimal total cell number Maximal volume Maximal total cell number
Chill 5 Rack¹
Direct MicroBeads
human, rat, non-human primate
Positive selection or depletion 110 cells per 80µL 160µL 2×10 1600µL 2×10
Direct MicroBeads, mouse Positive selection or depletion 110 cells per 90µL 180µL 2×10 1800µL 2×10
Whole Blood MicroBeads Whole blood or bone marrow 1 Original volume 0.25mL 1 mL
Cell Isolation Kits Untouched isolation 210 cells per 40µL 160µL 4×10 800µL 2×10
Cell Isolation Kits Untouched isolation 310 cells per 30µL 120µL 4×10 600µL 2×10
Chill 15 Rack²
Direct MicroBeads
human, rat, non-human primate
Positive selection or depletion 110 cells per 80µL 160µL 2×10 5200µL 6.5×10
Direct MicroBeads, mouse Positive selection or depletion 110 cells per 90µL 180µL 2×10 5850µL 6.5×10
Whole Blood MicroBeads Whole blood or bone marrow 1 Original volume 1mL 4mL
Cell Isolation Kits Untouched isolation 210 cells per 40µL 160µL 4×10 2600µL 6.5×10
Cell Isolation Kits Untouched isolation 310 cells per 30µL 120µL 4×10 1950µL 6.5×10
Chill 50 Rack³
Whole Blood MicroBeads Whole blood or bone marrow 1 Original volume 4mL 8mL
¹ Max. number of samples: 6; min. rst incubation volume: 0,2 mL; max. nal labeling volume: 2 mL
² Max. number of samples: 5; min. rst incubation volume: 0,2 mL; max. nal labeling volume: 6,5 mL
³ Max. number of samples: 3; min. rst incubation volume: 4 mL; max. nal labeling volume: 8 mL.
*When working with fewer cells than the necessary minimal volume, resuspend cells in the stipulated minimal volume.
autoMACS® Pro Separator
Short instructions – sample dilution
140-002-015.07

Consult the User Manual before operating this instrument for the rst time. Unless otherwise specically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, MACS, and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its aliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its aliates. All rights reserved.
Chill rack specifications Daily maintenance and rinsing programs
Periodic maintenance
Program Description Recommended usage Duration
Qrinse Standard short rinse of separation
columns and tubing system with
Running Buffer
Between separations of
frequent cells (>5 %)
1.5 min
Rinse Rinse of separation columns and
tubing system with Washing Solution
and Running Buffer
Prior to first separation 4 min
Clean Rinse of Rinse of separation columns
and tubing system with storage solution,
Washing Solution, and Running Buffer
After whole blood and
bone marrow applications.
7 min
Sleep Rinse with Washing Solution followed
by filling with storage solution
Before switching off the
autoMACS Pro Separator
5 min
Buffer consumption
Program Washing
Solution
Running
Buffer
Storage
solution
MACS Bleach
Solution
Time
Qrinse – 48 mL – – 1.5 min
Rinse 96 mL 48 mL – – 4 min
Clean 96 mL 48 mL 48 mL – 7 min
Sleep 96 mL – 48 mL – 5 min
Safe 96 mL 96 mL 40 21 min
Store 96 mL – 96 mL – 8 min
Col_ex 96 mL 96 mL – – 6 min
Action Description Recommended usage Duration
Column exchange using
(Col_ex program)
Replacement of separation
columns
Every two weeks OR after 100
separations, whichever
comes first
6 min
Running the Safe program Decontamination procedure
with MACS Bleach Solution
Every 3–6 months 21 min
Cleaning the pump syringe Cleaning of pump syringe
(refer to user manual)
Every 1–3 months
Running the Store program Rinse with Washing Solution,
followed by storage solution;
replacement of columns with
substitutes
Before storing the instrument
for a period longer than two
weeks
Rack type
and symbol
Slots Maximal
number
of samples
Manual labeling Autolabeling
Maximal
sample volume
Minimal first
incubation volume
Maximal final
labeling volume
Chill 5 24×5 mL 6
(5 mL
tubes)
2.5 mL 0.2 mL
0.25 mL*
2.0 mL
1 mL*
Chill 15 15×15 mL
5×5 mL
5
(15 mL
tubes)
12.5 mL 0.2 mL
1 mL*
6.5 mL
4 mL*
Chill 50 6×50 mL
3×15 mL
3×5 mL
3
(50 mL
tubes)
50 mL 4 mL*8 mL*
* Volumes refer to whole blood samles only.
autoMACS® Pro Separator
Short instructions