VWR avantor PH-2500L User manual

Instruction manual
VWR® pH-METER PH-2500L
EU cat. no 662-2 80
Version 1.1 Issue: 12.2021

Legal address of Manufacturer
Europe
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-300 Leuven
+32 63850
be.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE 7 4XN
uk.vwr.com
Country of Origin
POLAND

CONTENT
I. Introduction......................................................................................................................1
. Package content.........................................................................................................2
2. Disclaimer...................................................................................................................2
3. Characteristics of the meter........................................................................................3
4. The outside view.........................................................................................................4
5. The meter‘ s maintenance..........................................................................................6
6. Switching the meter on and off...................................................................................7
6. . Choosing the measurement function................................................................8
6.2. Stabilised reading.............................................................................................9
7. Preparation to work....................................................................................................9
7. . Choosing the kind of the temperature compensation......................................9
II. pH measurement...........................................................................................................10
8. Preparation of the pH electrode...............................................................................
9. Setting the pH measurement parameters................................................................
9. . Resolution....................................................................................................... 2
9.2. Electrode number........................................................................................... 2
9.3. Calibration solution......................................................................................... 3
9.4. Calibration points and date............................................................................. 3
9.5. Calibration validity date.................................................................................. 3
0. Calibration............................................................................................................... 4
0. . Calibration in buffer or standard solutions.................................................... 5
0.2. Entering the buffers’ values into the meter’s memory.................................. 6
0.3. Calibration in buffer solutions....................................................................... 8
0.4. Calibration with use of standard solutions....................................................20
0.5. Calibration with manual temperature compensation....................................2
. Checking the electrode condition...........................................................................22
2. pH measurement....................................................................................................23
2. . Measurement with automatic temperature compensation...........................23
2.2. Measurement with manual temperature compensation...............................24
III. Redox potential and temperature measurement......................................................2
3. Setting the redox potential parameters..................................................................26
3. . Resolution.....................................................................................................27
3.2. Relative measurement..................................................................................27
3.3. Reference voltage........................................................................................27
4. Redox potential measurement...............................................................................28
5. Setting the temperature measurement parameters...............................................29
5. . Resolution.....................................................................................................29
5.2. Probe number...............................................................................................30
5.3. Sensor group................................................................................................30
5.4. Unit...............................................................................................................30
5.5. Temperature of the manual compensation..................................................30
6. Temperature measurement....................................................................................3
IV. Options.........................................................................................................................32
7. Option screen.........................................................................................................33
7. . Function........................................................................................................33
7.2. Series............................................................................................................34
7.3. Miscellaneous...............................................................................................34
7.4. Info................................................................................................................36
8. Recording the readings, memory readout..............................................................37
8. . Storage parameters......................................................................................37
8.2. Entering single readings into the memory....................................................38

8.3. Collecting measurement series....................................................................39
8.4. Mode of holding readings (HOLD)................................................................40
8.5. Viewing the readings....................................................................................4
9. Calibration report....................................................................................................42
20. Communication with a PC......................................................................................42
2 . Technical data........................................................................................................44
22. Ordering information...............................................................................................45
23. Technical service....................................................................................................46
24. Warranty.................................................................................................................46
25. Equipment disposal................................................................................................47

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I. INTRODUCTION

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1. PACKAGE CONTENT
. PH-2 00L pH meter.
2. Glass combination pH electrode.
3. Stainless steel temperature sensor
4. pH 4,00, pH 7,00, pH 0,00 buffer solution, 50 ml each.
5. Electrolyte – saturated KCl – 50 ml.
6. 5V/ 000mA power adapter.
7. Free standing electrode holder.
8. USB cable for connecting with a PC
9. Cartoon box for the meter with accessories.
2. DISCLAIMER
Dear User!
Wide range of functions requires careful reading of the manual, in other
case some of the features may stay unused or using the meter may be
troublesome.
Using electrodes and cells of good quality and replacing them after a suitable
time provides obtaining high measuring accuracy. It is worth remembering that
electrodes have much shorter life time than the meter.
Clean membrane is the main condition of correct readings. After each
measurement the membrane should be rinsed with distilled water. Oil, pastes,
grease, etc., should be removed according to the instructions provided in the
electrode’s manual.
We wish a pleasant and trouble-free work with our meter.
User‘s manual for PH-2 00L pH meter v .0x

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3. CHARACTERISTICS OF THE METER
PH-2 00L waterproof pH-meter belongs to the newest generation of
measuring devices. The meter provides high accuracy and repeatability of
readings. The meter’s memory is independent from power supply. The meter
is equipped with a large backlit custom LCD, which can simultaneously display
pH and temperature readings. Thanks to its touchscreen keyboard the
operation of the meter is very comfortable.
The most important features of PH-2 00L are:
- automatic or manual temperature compensation;
- - 3 point pH electrode calibration;
- automatic recognition of pH buffers and standards;
- default values of buffer solutions which may be changed by the user;
- automatic change of the stored pH value of the NIST standard solution with
the temperature change (NIST norm);
- information about the pH electrode condition;
- storing of the calibration date and parameters of three electrodes in each
function;
- possibility of introducing the date of calibration validity termination and
signalling its expiry;
-signalising of the reading stabilisation (READY);
-holding the reading on the display (HOLD);
-storing measurement results with time, date and temperature, taken as
single or series of measurements with set time interval;
-possibility to create the last calibration report or reading of last 0 calibration
data in the data transmission software;
-USB output for connecting with PC;
- graphic display with brightness control and touchscreen;
- real time clock with date;
- energy saving mode activated after time set by the user.

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4. THE OUTSIDE VIEW
On the front wall of the meter there is a graphic LCD placed (Pic. ), on which
the following readings are displayed:
-pH or redox potential in mV units;
-temperature.
At the top of the LCD the current time and date are displayed.
Choosing particular functions is described in the chapter 7. . Symbols of the
units are displayed next to the readings.
Pic. .
The automatic temperature compensation is signalised by the symbol, the
manual compensation – by the symbol (next to the temperature reading).
Number of the chosen electrode is displayed on the left (E1, E2 or E3). It
informs, which of the recorded characteristics will be taken into consideration
during all calculations. Red colour of the electrode number informs about
erased characteristic, yellow – calibration validity date expiry, or the electrode
efficiency loss detected during the last calibration. The parameter screen of
each function displays all parameters entered by the user.
User‘s manual for PH-2 00L pH meter v .0x

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The button (Pic. 2) placed below the display is used for switching the
meter on and off.
In the back wall of the meter the sockets are placed with the symbols given
below:
pH/mV -BNC- 0 socket for connecting the combination pH or redox
electrode;
temp -RCA (Chinch) socket to connect the temperature probe;
USB -USB socket for connecting with a PC;
power -DC2. socket for connecting the power adapter;
Pic. 2.

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. THE METER‘ S MAINTENANCE
The meter is equipped with a graphic touchscreen. The maintenance consists
in pressing particular keys and windows which appear on the screen. Grey
captions in the windows and keys inform that these elements are not active in
this particular operating mode. Particular keys, connected with the
measurement function (i.e. or ) become active after selecting
the chosen function. To select, press the screen in a freely chosen spot of the
chosen function. Around this spot a frame will be displayed. To deselect,
press the spot again. If the chosen function has a possibility of calibration, the
key becomes active. If it has additional parameters, the key
also becomes active. The key is the only key in the meter that
responds exclusively to a long press (about 2 sec.). It prevents from
accidental erase of the electrode (cell) calibration data.
Entering wrong value, attempt of calibration when the meter doesn‘ t
recognise the standard or attempt to turn the meter off when series collecting
proceeds are signalised by a triple warning sound, irrespective of the chosen
sound settings.
Turn the meter on and off with one mechanical button. When a
measurement series is collected, the button is not active until the
process of collecting ends or is stopped.
User‘s manual for PH-2 00L pH meter v .0x

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6. SWITCHING THE METER ON AND OFF
After switching the meter on with the button, the memory test proceeds.
If the test ends successfully, the meter enters the measurement screen with
settings entered before previous switching it off. In case of detecting the
manufacturer‘s calibration data loss, the following information will appear:
Pressing the button accepts standard manufacturer‘s calibration
parameters and enters the measurement screen. Such situation will be
repeating each time the meter is switched on and requires sending the meter
to the manufacturer for servicing.
In case of the user‘s data loss (i.e. the pH electrode characteristic), the
following information will appear:
Pressing the button accepts and records in the memory standard pH
electrode characteristic and enters the measurement screen. In such case,
calibration of the pH electrode is necessary. Repeating of the situation after
switching the meter on again informs about the EEPROM memory
malfunction and requires sending the meter to the manufacturer for servicing.
Switch the meter off by pressing the button. During the process of
collecting series it is impossible to turn the meter off.

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6.1. Choosing the measurement function
The user may choose the reading to be displayed on the measuring screen:
pH or redox potential. To choose, on the measuring screen press the
button, the option screen will display with the FUNCTION tab open containing
the previously set configuration (Pic. 3A).
Pic. 3
After pressing the window with the chosen function, the confirmation mark will
be displayed next to it.
The window for choosing the stabilised reading signalisation mode is placed
below (Pic. 3B):
- the stabilised reading signalisation is off;
- the stabilised reading signalised with changing the frame
colour from white to green (only for reading marked with a
frame);
- the stabilised reading signalised by colour change and sound.
Return to the measurement screen by pressing the button.
User‘s manual for PH-2 00L pH meter v .0x

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6.2. Stabilised reading
The stabilised reading signalisation is active only for the reading marked with
a frame. If the measured value meets the criterium of the stabilised reading, it
is signalised by green colour of the frame and additionally with a sound if it
has been activated. In this mode the meter does not record changes of the
measured value in a certain range and does not display them. The meter
exits the stabilised reading mode after exceeding this range, the frame
changes colour to white and the meter displays the current measurement
value. In practice, lowering accuracy in this mode is insignificant.
7. PREPARATION TO WORK
Before starting the work:
- connect the power adapter to the power socket;
-connect prepared combination pH electrode or redox electrode to the
pH/mV (BNC-50) socket;
-in case of using the temperature probe connect it to the temp (RCA)
temperature socket;
-in case of working with a PC connect a suitable cable to the USB socket;
-switch the meter on by pressing the button.
7.1. Choosing the kind of the temperature compensation
The meter switches it self to the automatic or manual temperature
compensation mode. Connecting the temperature probe switches the
automatic temperature compensation on. Next to the reading the symbol is
displayed. After disconnecting the probe the meter enters the manual
temperature compensation mode. Instead of the symbol the is displayed.
In case of the manual temperature compensation the temperature value is set
on the temperature measurement parameters screen (point 5.5).

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II. pH MEASUREMENT
User‘s manual for PH-2 00L pH meter v .0x

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8. PREPARATION OF THE pH ELECTRODE
The electrode should be prepared to work according to the manufacturer’s
instructions. It is especially important to keep the membrane active and
clean, rinse it accurately after each measurement and dry it gently with a
tissue paper.
9. SETTING THE pH MEASUREMENT PARAMETERS
Enter the pH measurement parameters setting screen by pressing the pH
measurement reading (a frame displays on it - Pic. 4A), and next the
button. The screen (Pic. 4B) enables choosing the resolution, electrode
number, checking the solution type and - after pressing the button -
(Pic. 4C) calibration points and validity time (these parameters are entered in
the calibration mode only).
Pic. 4

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When the parameters are set, return to the measurement screen by pressing
the button.
9.1. Resolution
The reading can be displayed with low or high resolution. By pressing the
RESOLUTION window, choose:
- 0.0 pH measurement resolution;
- 0.00 pH measurement resolution.
9.2. Electrode number
If the meter stores more than one electrode’s characteristic, the electrodes
may be replaced without calibration. This option is very useful for work with
different types of samples. After pressing the ELECTRODE No. window the
table with electrode numbers appears (Pic. 5). Choose one of the
characteristics stored under E1, E2 or E3 number.
Pic. 5
The colour if the number‘s informs about the electrode‘s condition:
white - electrode efficient, calibration valid;
yellow - electrode efficiency loss - detected during the last calibration or
the calibration validity date expired (see chapter );
red - characteristic erased, the electrode calibration necessary.
In the Pic. 5, the E1 electrode is efficient, the E2 electrode has the
characteristic erased and the E3 electrode lost its efficiency or the calibration
validity date expired.
User‘s manual for PH-2 00L pH meter v .0x

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9.3. Calibration solution
Displays types of calibration solutions applied for the last calibration:
- calibration with manually entered buffers‘ values;
- calibration with automatic correction of the standards‘ values.
Calibration in buffers and standards is described in detail in the chapter 0.
The types of calibration solutions are stored for each of the three
electrodes separately and may be changed only in the calibration mode
(point 10.2).
9.4. Calibration points and date
After pressing the button the following parameters are displayed
(Pic. 4C): the electrode condition, calibration points, validity time and date of
the last calibration. Markers next to the calibration points windows inform that
the last calibration was performed in these particular solutions. All the
parameters‘ values are stored separately for each of the three electrodes.
The calibration points may be changed only after entering the
calibration mode (point 10.2).
9. . Calibration validity date
The meter stores the calibration validity time for each of the three electrodes
separately. Expiry of this date is signalised with yellow electrode number. To
set the calibration validity expiry date, press the VALIDITY window, the
numerical keyboard will appear to enter the number of days and confirm with
the button.

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10. CALIBRATION
Before starting measurement with a new electrode or before making
measurements which require high accuracy, the electrode connected to the
meter should be calibrated. Results of measurements made without
calibration will be burdened with a significant error. Calibration is performed in
the buffer or standard solutions. It consists in comparing pH value of the
standard solutions with the reading displayed by the meter and automatic
correction which is taken into consideration during the next measurements.
Calibration should be periodically repeated because the parameters of the
electrode in use are changing, what influences the accuracy. The frequency
of this procedure depends on the required accuracy, number of the
measurements carried out, conditions in which the electrode was used,
temperature and value of the measured solutions.
PH-2 00L enables storing characteristics of three calibrated pH electrodes
separately, recorded under different numbers (E1, E2 or E3). This feature is
very useful when it is necessary to change the electrode quickly or to replace
a broken one.
The meter enables entering the calibration validity expiry date for each of the
electrodes separately. If this option is active, the calibration should be
performed when the applied electrode number (E1, E2 or E3) turns yellow.
For obtaining optimal calibration results, the pH values of the applied
solutions should be entered to the meter’s memory by the user. During
calibration, after putting the pH electrode and the temperature probe into
solution, the meter detects its pH value automatically. When the highest
accuracy is required, it is recommended to use certified standard solutions.
However, the most often used are buffer solutions of total values i.e. 2.00 pH,
4.00 pH etc, with a composition specified by the manufacturer. They are also
of quite high accuracy.
For accurate measurements it is necessary to use fresh solutions of good
quality.
The temperature changes have a great influence on the pH value of
standards and buffer solutions. The manufacturers usually specify the pH
values of a solution in a specific temperature. During accurate calibration the
stored solution value has to be the same as the value of this solution in the
temperature in which the calibration is performed. If the electrode number
turns yellow after the calibration is finished, it informs that the electrode lost
its efficiency and will have to be replaced soon. An additional information is
shown on the electrode calibration points screen (description in the
chapter ).
User‘s manual for PH-2 00L pH meter v .0x

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Calibration with use of one solution does not guarantee high accuracy. If only
one solution is used, its value should be close to the anticipated value of the
measured solution. If the required accuracy isn’t very high and the
measurements are made in the whole range, -point calibration should be
performed with use of solution close to 7.00 pH. It enables to avoid an error
resulting from so called zero offset of the electrode. In other points the meter
adopts standard characteristic from the memory.
The solutions may be used randomly. In PH-2 00L the electrode
characteristic is approximated linearly between the calibration points.
Entering the calibration mode erases the electrode’s characteristic
stored under the chosen number.
There is no possibility to calibrate the electrode only in one point with
leaving the rest of the data from the previous calibration.
The characteristic erase is signalised by red colour of the electrode number.
10.1. Calibration in buffer or standard solutions
Before starting the calibration process, prepare the meter according to the
chapter 6 and decide whether the calibration will be performed with use of
buffer or standard solutions.
The calibration may be performed in two following methods:
1. Entering the values of currently used pH buffers to the meter’s
memory; calibration is performed in these buffers.
2. Using the pH standard solutions values entered to the memory by
the manufacturer (NIST norm conformity). Choosing this type of
calibration automatically enables correction connected with the
temperature influence on the standard’s value. As a result, there is
no need to adjust the standard’s temperature or to enter the
standards’ pH values corresponding with different temperature
values.

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10.2. Entering the buffers’ values into the meter’s memory
If the calibration with use of buffers has been chosen and the pH values set by
the manufacturer are used, there is no need to change them. However, it
should be verified whether the values correspond to those of applied buffers.
Different buffers’ values should be entered to the meter’s memory before
calibration.
To enter:
-according to the point 9.2 choose the number of electrode (E1, E2 or E3),
for which the points of calibration are to be changed;
-enter the calibration mode: select the pH measurement on the
measurement screen, press and hold the button until the
background turns red (Pic. 6A). The previous characteristic is erased;
-press the button, the pH measurement parameters screen will
appear (Pic. 6B);
-press the SOLUTION window and choose BUFFER;
-press the button, the screen with calibration points will appear (Pic.
6C);
-select the window with a point to be changed, a numerical keyboard will
appear to enter the value and confirm with the button.
-return to the measurement screen in calibration mode by double press of
the button and calibrate the electrode in the chosen points or exit
the calibration mode by pressing the button again;
User‘s manual for PH-2 00L pH meter v .0x