Waters ACQUITY UPLC H-Class Series Parts list manual

ACQUITY UPLC H-Class Series
System Guide
715005702
Revision A
Copyright © Waters Corporation 2018
All rights reserved

General information
Copyright notice
© 2018 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN
IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE
REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and should not be construed
as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any
errors that may appear in this document. This document is believed to be complete and accurate
at the time of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the most recent revision
of this document, consult the Waters website (waters.com).
Trademarks
ACQUITY® is a registered trademark of Waters Corporation.
ACQUITY UPLC® is a registered trademark of Waters Corporation.
Auto•Blend PlusTM is a trademark of Waters Corporation.
eCordTM is a trademark of Waters Corporation.
Empower® is a registered trademark of Waters Corporation.
KEL-F® is a registered trademark of 3M.
Keps® is a registered trademark of Illinois Tool Works Inc.
LAC/ETM is a trademark of Waters Corporation.
MassLynx® is a registered trademark of Waters Corporation.
Millennium® is a registered trademark of Waters Corporation.
MP35NTM is a trademark of SPS Technologies Inc.
PEEKTM is a trademark of Victrex PLC.
PHILLIPS® is a registered trademark of Phillips Screw Company.
PIC® is a registered trademark of Waters Corporation.
Waters® is a registered trademark of Waters Corporation.
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Teflon® is a registered trademark of E.I. du Pont de Nemours and Company or its affiliates.
THE SCIENCE OF WHAT'S POSSIBLE® is a registered trademark of Waters Corporation.
TORX® is a registered trademark of Acument Intellectual Properties, LLC in the United States or
other countries.
TritonTM X-100 is a trademark of The Dow Chemical Company or an affiliated company of Dow.
TWEENTM is a trademark of ICI Americas Inc.
UNIFI® is a registered trademark of Waters Corporation.
Waters® is a registered trademark of Waters Corporation.
All other trademarks are property of their respective owners.
Customer comments
Waters’ Customer Experience and Knowledge Management organization invites you to report any
errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us
better understand what you expect from our documentation so that we can continuously improve
its accuracy and usability.
We seriously consider every customer comment we receive. You can reach us at
[email protected].
Contacting Waters
Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the Internet,
telephone, fax, or conventional mail.
Waters contact information
Contacting medium Information
Internet The Waters Web site includes contact information for Waters locations
worldwide.
Visit www.waters.com
Telephone and fax From the USA or Canada, phone [phone], or fax [phone].
For other locations worldwide, phone and fax numbers appear in the
Waters Web site.
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Waters contact information (continued)
Contacting medium Information
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose chemical,
biological, or radiological hazards (or any combination thereof). You must know the potentially
hazardous effects of all substances you work with. Always follow Good Laboratory Practice
(GLP), and consult your organization’s standard operating procedures as well as your local
requirements for safety.
System height
Warning: To avoid injury, do not stack modules, including the solvent tray and rails,
higher than one meter (39.4 inches) above the bench top.
Warning: To avoid spinal and muscular injury, do not attempt to lift a system module
without assistance.
Warning: To avoid crushing your fingers beneath or between modules, use extreme
care when installing a module in the system stack.
Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is used to find out the
nature of the potential hazard and any actions which have to be taken.
Power cord replacement hazard
Warning: To avoid electric shock, use SVT-type power cords in the United States and
HAR-type (or better) cords in Europe. The power cords must be replaced only with ones
of adequate rating. For information regarding which cord to use in other countries,
contact your local Waters distributor.
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High voltage hazard
Warning: To avoid electric shock, do not remove protective panels from system
modules. The components within are not user-serviceable.
Bottle placement prohibition
Warning: To avoid injury from electrical shock or fire, and damage to the equipment, follow
these guidelines:
• Do not expose the workstation or ancillary equipment to dripping or splashing liquids.
• Do not place objects filled with liquid, such as solvent bottles, on top of the workstation or
ancillary equipment.
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for compliance, could
void the user's authority to operate the equipment. This device complies with Part 15 of the FCC
Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful
interference, and (2) this device must accept any interference received, including interference that
may cause undesired operation.
Electrical power safety notice
Do not position the device so that it is difficult to disconnect the power cord.
Equipment misuse notice
If equipment is used in a manner not specified by its manufacturer, protections against personal
injury inherent in the equipment’s design can be rendered ineffective.
Safety advisories
Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning
advisories and notices.
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Operating the system
When operating the system, follow standard quality-control (QC) procedures and the guidelines
presented in this section.
Applicable symbols
The following symbols can be present on the device, system, or packaging.
Symbol Definition
Manufacturer
Date of manufacture
Authorized representative of the European Community
Confirms that a manufactured product complies with all applicable
European Community directives
or
Australia EMC compliant
Confirms that a manufactured product complies with all applicable United
States and Canadian safety requirements
Consult instructions for use
Alternating current
Electrical and electronic equipment with this symbol may contain
hazardous substances and should not be disposed of as general waste.
For compliance with the Waste Electrical and Electronic Equipment
Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct
disposal and recycling instructions.
Serial number
REF
Part number, catalog number
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Audience and purpose
This guide is intended for personnel who operate and maintain the ACQUITY UPLC H-Class
Series system. The term "Series" refers to both the latest generation ("PLUS") and previous
generations of H-Class systems. It gives an overview of the system’s technology and operation.
Intended use
Waters designed this system to perform liquid chromatography separations in these
environments:
• Pharmaceutical development and discovery
• Quality assurance and quality control
• Chemical materials
• Environmental
• Food safety
The system is not intended for use in diagnostic applications.
Calibrating
To calibrate LC systems, adopt acceptable calibration methods using at least five standards to
generate a standard curve. The concentration range for standards must include the entire range
of QC samples, typical specimens, and atypical specimens.
When calibrating mass spectrometers, consult the calibration section of the operator’s guide for
the instrument you are calibrating. In cases where an overview and maintenance guide, not an
operator’s guide, accompanies the instrument, consult the system guide or the instrument’s
online Help system for calibration instructions.
Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a
compound. If sample trays are the same or very similar, vary the location of the QC samples in
the trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision
from day to day and run to run. Data collected when QC samples are out of range might not be
valid. Do not report these data until you are certain that the instrument performs satisfactorily.
EMC considerations
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Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.
ISM classification: ISM group 1 class B
This classification has been assigned in accordance with CISPR 11 Industrial Scientific and
Medical (ISM) instrument requirements.
Group 1 products apply to intentionally generated and/or used conductively coupled radio-
frequency energy that is necessary for the internal functioning of the equipment.
Class B products are suitable for use in both commercial and residential locations and can be
directly connected to a low voltage, power-supply network.
EC authorized representative
Address Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK
Telephone [phone]
Fax [phone]
Contact Quality manager
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Table of contents
General information .......................................................................................................ii
Copyright notice ..................................................................................................................................... ii
Trademarks............................................................................................................................................ ii
Customer comments..............................................................................................................................iii
Contacting Waters .................................................................................................................................iii
Safety considerations............................................................................................................................ iv
System height................................................................................................................................. iv
Safety hazard symbol notice .......................................................................................................... iv
Power cord replacement hazard..................................................................................................... iv
High voltage hazard......................................................................................................................... v
Bottle placement prohibition ............................................................................................................ v
FCC radiation emissions notice....................................................................................................... v
Electrical power safety notice.......................................................................................................... v
Equipment misuse notice ................................................................................................................ v
Safety advisories ............................................................................................................................. v
Operating the system............................................................................................................................ vi
Applicable symbols......................................................................................................................... vi
Audience and purpose....................................................................................................................vii
Intended use...................................................................................................................................vii
Calibrating ......................................................................................................................................vii
Quality control.................................................................................................................................vii
EMC considerations..............................................................................................................................vii
Canada spectrum management emissions notice.........................................................................viii
ISM classification: ISM group 1 class B.........................................................................................viii
EC authorized representative ..............................................................................................................viii
1 ACQUITY UPLC H-Class Series System.................................................................13
1.1 UPLC technology ..........................................................................................................................13
1.2 Features of the ACQUITY UPLC H-Class Series system .............................................................15
1.2.1 Software features.................................................................................................................16
1.3 System components......................................................................................................................17
1.3.1 Quaternary solvent manager (QSM)....................................................................................19
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1.3.2 Sample manager-flow through needle (SM-FTN)................................................................19
1.3.3 Column heater (CH-A).........................................................................................................20
1.3.4 Column manager (optional) .................................................................................................20
1.3.5 Column Module Switch Box (optional).................................................................................21
1.3.6 Sample organizer (optional).................................................................................................21
1.3.7 FlexCart (optional) ...............................................................................................................22
1.3.8 Post-Injection Volume Kit (optional).....................................................................................22
1.3.9 Detection..............................................................................................................................22
1.3.10 Column technology............................................................................................................22
1.4 For additional information..............................................................................................................23
2 Performance optimization........................................................................................25
2.1 General guidelines ........................................................................................................................25
2.1.1 Follow these general recommendations when performing a UPLC analysis.......................26
2.2 Dispersion .....................................................................................................................................27
2.3 Carryover ......................................................................................................................................27
2.4 Cycle time (between injections) ....................................................................................................28
2.5 Preventing leaks............................................................................................................................28
2.5.1 Installation recommendations for fittings .............................................................................29
2.6 Developing methods .....................................................................................................................42
2.7 Sample preparation.......................................................................................................................42
2.7.1 Particulates..........................................................................................................................42
2.7.2 Matching sample diluents ....................................................................................................42
2.8 Transferring methods ....................................................................................................................43
2.8.1 Columns calculator .............................................................................................................45
2.8.2 Transferring from HPLC to UPLC........................................................................................45
2.8.3 Transferring from UPLC to HPLC........................................................................................45
2.9 Solvent recommendations.............................................................................................................46
3 System preparation ..................................................................................................47
3.1 Powering-on the system................................................................................................................47
3.2 Opening the console .....................................................................................................................48
3.2.1 To open the console from Empower software .....................................................................48
3.2.2 To open the console from MassLynx software ....................................................................48
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3.2.3 To open the console from UNIFI software...........................................................................49
3.3 Priming the system........................................................................................................................49
3.4 Monitoring module LEDs...............................................................................................................49
3.4.1 Power LED...........................................................................................................................49
3.4.2 Status LEDs.........................................................................................................................49
3.5 Enabling the leak sensors .............................................................................................................51
3.6 Monitoring from control panels......................................................................................................52
3.6.1 Column manager control panel............................................................................................52
3.6.2 Sample manager control panel............................................................................................53
3.6.3 Quaternary solvent manager control panel..........................................................................55
3.6.4 ISM control panel.................................................................................................................56
3.6.5 ELS control panel ................................................................................................................58
3.6.6 FLR control panel ................................................................................................................59
3.6.7 PDA control panel................................................................................................................60
3.6.8 RI control panel....................................................................................................................61
3.6.9 TUV control panel................................................................................................................62
3.6.10 ACQUITY QDa ..................................................................................................................63
3.6.11 2432 conductivity control panel .........................................................................................64
3.7 Starting up the system ..................................................................................................................64
4 System maintenance................................................................................................67
4.1 Contacting Waters Technical Service ..........................................................................................67
4.1.1 Viewing module information.................................................................................................67
4.2 Maintenance procedures and frequency.......................................................................................68
4.3 Spare parts....................................................................................................................................68
4.4 Configuring maintenance warnings...............................................................................................68
5 External connections ...............................................................................................69
5.1 Ethernet connections ....................................................................................................................69
5.2 External cable connections ...........................................................................................................70
5.3 Plumbing connections ...................................................................................................................71
5.4 Waste-tubing connections.............................................................................................................72
5.5 Electricity source ...........................................................................................................................73
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5.5.1 Connecting to a wall electricity source.................................................................................73
5.5.2 Connecting to a cart's electricity source ..............................................................................74
5.6 Connecting signal cables ..............................................................................................................74
5.7 Connecting to a column module....................................................................................................76
A Post-Injection Volume Kit instructions..................................................................78
B Safety advisories .....................................................................................................80
B.1 Warning symbols ..........................................................................................................................80
B.1.1 Specific warnings ................................................................................................................81
B.2 Notices..........................................................................................................................................82
B.3 Bottles Prohibited symbol .............................................................................................................83
B.4 Required protection ......................................................................................................................83
B.5 Warnings that apply to all Waters instruments and devices .........................................................83
B.6 Warnings that address the replacement of fuses .........................................................................87
B.7 Electrical symbols .........................................................................................................................89
B.8 Handling symbols .........................................................................................................................90
B.9 Stacking system modules with interlocking features ....................................................................90
B.10 Stacking system modules without interlocking features .............................................................91
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1ACQUITY UPLC H-Class Series
System
The ACQUITY UPLC H-Class Series system was designed to support HPLC, UHPLC, and UPLC
methods. The low dispersion of the system allows you to maximize chromatographic resolution
for the most challenging and complex separations. Software and hardware tools enable simplified
transfer of methods and support automated method development.
1.1 UPLC technology
In 2004, Waters made significant advances in instrumentation and column design to introduce
UPLC technology to the field of separation science. By employing this technology, Waters’
ACQUITY UPLC systems achieve a marked increase in resolution, speed and sensitivity in liquid
chromatography when compared to conventional systems.
UPLC technology is based on columns packed with 1.7 µm-diameter, spherical particles coupled
with low dispersion systems, allowing you to realize the full separation potential of these highly
efficient columns.
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Figure 1–1: Evolution of particle size in liquid chromatography and the impact on
separation efficiency
It is apparent from the figure, above, that using 1.7-µm particles achieves higher efficiency that
persists as flow rate increases (lower HETP indicates higher efficiency). When operating in this
area of the plot, the peak capacity and the speed of a separation can set limits well beyond those
of conventional HPLC technology.
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Figure 1–2: Comparison of chromatographic separations using 5.0-µm and 1.7-µm
particles
The figure above compares two separations, one using HPLC with a column packed with 5 µm
particles, and the other using UPLC with a column packed with 1.7 µm particles. The
improvements in both resolution and the speed of analysis are apparent in the UPLC
chromatogram. Each separation was performed on a 2.1 × 50 mm column. Chromatographic
conditions for the separations were identical, except for the flow rate, which was scaled based on
particle size.
1.2 Features of the ACQUITY UPLC H-Class Series system
The ACQUITY UPLC H-Class Series system combines the speed and performance of UPLC with
the ability to run HPLC separations. This combination provides many benefits, including these:
• High-pressure, small-particle chromatography allowing faster, higher-resolution analyses,
compared with conventional HPLC
• Low solvent consumption (significantly less than conventional HPLC)
• Flexibility in solvent mixing by using a quaternary solvent manager
• A flow-through-needle sample manager
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• Pump and sample manager design enhancements to minimize dispersion and reduce cycle
time
• Flexible column management options to support different column lengths and automated
switching of up to six columns in independent temperature zones
• An optional sample organizer to expand sample capacity
1.2.1 Software features
1.2.1.1 Quantum Synchronization
Introducing a low-pressure sample into the high-pressure fluid stream during injection causes a
pressure pulse that can affect chromatographic results. The Quantum Synchronization feature
reduces the effect of this pressure pulse. The sample manager and solvent manager
communicate to automatically coordinate the injection sequence, enabling the solvent manager to
provide additional pressure at the exact moment the sample manager switches its injector valve
to the inject position, to introduce the low-pressure sample.
1.2.1.2 Gradient Smart Start
Before each sample injection, a sample manager typically performs wash sequences and then
aspirates the appropriate sample volume. When these tasks are completed, the solvent manager
begins to deliver the gradient to the injection valve. The dwell volume of the system, which affects
the amount of time required for this gradient to reach the column, can be a significant component
of the overall cycle time.
The Gradient Smart Start feature adjusts when an injection is made relative to when it starts. In
this way, when you transfer methods, the feature compensates for differences in dwell volume
between chromatographic systems. Moreover, it automatically coordinates all pre-injection
operations, minimizing delays that would increase the overall cycle time. In doing so, the feature
makes it possible to begin gradient operation before or during the sample manager's pre-injection
functions, resulting in significant time savings.
1.2.1.3 Wash Plungers
Precipitated material that remains on the solvent manager’s pump plungers can damage the high-
pressure seals. The Wash Plungers function washes the seals and plungers with seal wash
solvent, to remove any precipitate. You can use the Wash Plungers function as needed, or run
the function as part of the No-Flow Shutdown feature.
Tip: The Wash Plungers function is not available when the module is operating.
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1.2.1.4 No-Flow Shutdown
The No-Flow Shutdown feature runs the Wash Plungers function after the solvent manager
remains idle for a specified time interval. This feature prevents deposition of precipitated material
on the pump plungers and plunger seals while the system is idle.
1.2.1.5 Automatic Prime
When you enable the solvent manager's Automatic Prime function, the system primes the lines of
the optional solvent selection valve when a new line is selected. You can specify the flow rate and
duration of the prime for the new solvent line.
Example: If a first injection uses line D1 and a second injection uses line D2, the solvent
manager primes line D2 between the first and second injections.
1.2.1.6 Flow Ramping
Using the Flow Ramping function, you specify the rate at which the solvent manager increases or
decreases flow.
Tip: The default value is set to support rigid, HPLC and UPLC column particles. For pressure
sensitive columns (such as gel columns), the flow ramping should be adjusted.
1.2.1.7 Auto•Blend Plus
Auto•Blend Plus technology uses pure solvents and concentrated stocks to blend mobile phase
compositions at a specific pH. At the same time, it controls the concentration of salt or organic
solvent, to optimize separations. Use the Auto•Blend Plus feature to create and store buffer
systems in a solvent catalog that all users of an ACQUITY quaternary solvent manager can
share. To prepare and adjust chromatographic mobile phases, you add acid, base, salt, organic
solvent, or water to the solvent reservoirs. By doing so you can, for example, optimize protein
separations, which are especially sensitive to a buffer's pH and salt concentration. You can also
optimize reversed-phase separations that are sensitive to pH and organic-solvent composition.
See also:
•Auto•Blend Plus Technology for Ion Exchange, Size Exclusion, and Reversed-phase
Chromatography, included on the system documentation media.
• The Auto•Blend Plus videos on the Support tab on the Waters Auto•Blend Plus page.
1.3 System components
The following illustration depicts a system stack which includes four core modules and the solvent
bottle tray.
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Figure 1–3: Example of an H-Class Series system core stack
Solvent bottle tray
Detector
Column heater
Sample manager
Solvent manager
The system includes a solvent manager, sample manager, column heater, detector (tunable
ultraviolet, photodiode array, evaporative light scattering, fluorescent, conductivity, refractive
index, or mass spectrometry), and an ACQUITY UPLC column.
Waters Empower chromatography software, UNIFI, or MassLynx mass spectrometry software
controls the system.
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1.3.1 Quaternary solvent manager (QSM)
The quaternary solvent manager (QSM) is a low-pressure mixing, high-pressure pump. It
provides steady (pulse-free) solvent flow at analytical flow rates to 1 mL/min at 103,421 kPa
(1034 bar, 15,000 psi) and to 2.2 mL/min, at reduced pressures, to 53779 kPa (537 bar, 7800
psi). The QSM can pump four degassed solvents simultaneously using a gradient proportioning
valve (GPV) to dynamically create a specified composition.
Note: An optional 6-solvent selection valve can be added to the QSM line D for increased
solvent flexibility.
Note: An optional degasser vent line extension exists for applications requiring longer tubing
lengths. For more information, contact your local Waters distributor.
1.3.2 Sample manager-flow through needle (SM-FTN)
The sample manager-flow through needle (SM-FTN) uses a direct-injection mechanism to inject
samples drawn from plates and vials onto a chromatographic column. This injection style injects
all of the aspirated sample onto the column, without any sample volume overhead. The sample
needle is part of the fluidic path and the internal surfaces are flushed by the mobile phase during
the separation. The exterior of the needle is washed in the injection port as specified in the
method. The standard injection volume range of the SM-FTN is 10 µL, however this can be
expanded up to 1 mL through the addition of optional extension loops (installed between the
sample needle and the injection valve).
The SM-FTN also features several advanced sample conditioning features such as dilution, auto-
addition, and mixing, as well as load-ahead capabilities to reduce inject-to-inject cycle times.
1.3.2.1 Wash solvent
The sample manager needle wash system is used to minimize sample carryover. It uses a single
wash solvent and this solvent does not enter the flow path of the system.
Notice:
• Do not leave buffers stored in the system.
• Flush all flow paths, including the needle wash, with plenty of non-buffered solvent
before shutting down the system.
• For extended shutdown periods (longer than 24 hours), use 10% to 20% methanol
in water.
• When using a buffered wash solvent, prime it for a minimum of 30 sec.
• Use of buffers can cause salt build-up on the needle and wash port, which can
require periodic cleaning.
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1.3.2.2 Purge solvent
The primary function of the purge solvent is to move sample along the injection pathway. The
purge solvent also primes the sample syringe and injection pathway. The solvent’s injection onto
the column occurs only during auto-dilution, when it is used as the dilution solvent.
Notes:
• Purge solvent must be miscible with the needle wash solvent.
• Waters recommends a purge solvent of 90/10 Water/Methanol whenever possible.
1.3.3 Column heater (CH-A)
Column temperature variations can shift peak retention times and alter peak shapes, increasing
the difficulty of achieving precise results. The column heater (CH-A) compartment helps to ensure
precise, reproducible separations by controlling the column temperature.
The CH-A heats to any temperature from 20 ºC (or a minimum of 5 ºC above ambient
temperature) to 90 ºC. An active preheating device heats the incoming solvent before it enters the
column. The CH-A can accommodate columns up to 4.6 mm I.D. and up to 150 mm length.
Tips:
• Active preheating is the default configuration for the system.
• An optional, passive, column stabilizer is available for existing chromatographic methods not
suitable for active preheating.
1.3.4 Column manager (optional)
The ACQUITY UPLC H-Class Series column manager is an option for helping to ensure precise,
reproducible separations. The column manager can regulate the temperature of columns from 4
to 90 °C. Its troughs can accommodate columns of up to 4.6-mm I.D. and up to 150-mm length,
depending on the configuration. Each of the two column troughs can condition one 15-cm column
(with filter) or two 50-mm columns (without filters).
The column manager offers a waste channel as well as a bypass channel H and automated,
programmable switching between columns, for methods development.
You can configure the column manager to work with as many as two column manager auxiliary
modules, in addition to the base unit. The column manager auxiliary modules are controlled by
the column manager and operate in the same temperature range. Each of the two column
troughs can accommodate one column of up to 150 mm in length with a pre-column filter, or two
columns up to 500 mm in length without pre-column filters.
Note: A system configured with a column manager base unit (and no additional CM-AUX) can
accommodate as many as four columns (50-mm); a system with a column manager base unit and
one CM-AUX module can accommodate as many as four long columns; a system with a column
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