Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
Binary Pump
User Manual

Agilent 1290 Infinity Binary Pump User Manual
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© Agilent Technologies, Inc. 2010-2018
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Manual Part Number
G4220-90007 Rev. B
Edition
10/2018
Printed in Germany
Agilent Technologies
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Agilent 1290 Infinity Binary Pump User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1290 Infinity Binary Pump (G4220A).
1Introduction
This chapter gives an introduction to the pump, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your Agilent 1290 Infinity Binary Pump.
4UsingthePump
This chapter explains the operational parameters of the Agilent 1290 Infinity
Binary Pump.
5 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1290 Infinity Binary Pump User Manual
In This Guide…
8 Test Functions and Calibrations
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity Binary
Pump.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1290 Infinity Binary Pump User Manual 5
Contents
Contents
1Introduction 9
Features 10
Overview of the Binary Pump 11
Pump Principle 12
System Overview 15
2 Site Requirements and Specifications 17
Site Requirements 18
Physical Specifications 21
Specifications 22
3 Installing the Module 25
Unpacking the Module 26
Optimizing the Stack Configuration 27
Installation Information on Leak and Waste Handling 32
Removing the Transport Foam 36
Installing the Pump 37
Flow Connections to the Pump 41
Installation of Seal Wash Function 45
4UsingthePump 47
Magnets 48
Turn on/off 49
Status Indicators 51
Leak and Waste Handling 52
Best Practices 53
Preparing the Binary Pump 58
Algae Growth in HPLC Systems 59
Setting up the Pump with the Instrument Control Interface 60
Purging the Pump 69
Solvent Information 71
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260
Infinity II Flexible Pumps 78

6 Agilent 1290 Infinity Binary Pump User Manual
Contents
5 Optimizing Performance 83
Delay Volume and Extra-Column Volume 84
How to Configure the Optimum Delay Volume 85
How to Achieve Higher Resolution 87
Using Solvent Calibration Tables 90
6 Troubleshooting and Diagnostics 91
Available Tests vs User Interfaces 92
Agilent Lab Advisor Software 93
7 Error Information 95
What Are Error Messages 97
General Error Messages 98
Pump Error Messages 107
8 Test Functions and Calibrations 123
Introduction 124
Pump Leak Rate Test 125
System Pressure Test 130
9 Maintenance 133
Introduction to Maintenance 135
Warnings and Cautions 137
Overview of Maintenance 138
Cleaning the Module 139
Installing Fittings and Capillaries 140
Replacing the Shutoff Valve Panel 141
Replacing the Pressure Sensor 143
Replacing the Inlet valve 146
Replacing the Outlet Valve 148
Replacing the Solvent Selection Valve (SSV) 151
Changing configuration or replacing the Jet Weaver 153
Replacing the Seal Wash Pump 156
Releasing a Stuck Inlet Valve 157
Remove the Pump Head Assembly 160
Pump Head Maintenance (Tool Free) 163

Agilent 1290 Infinity Binary Pump User Manual 7
Contents
Install the Pump Head Assembly 178
Replacing the Purge Valve Head 180
Replacing Parts of the High Pressure Filter Assembly 183
Installing the Valve Rail Kit 185
Replacing the Main Power Fuses 186
Replacing Module Firmware 188
Preparing the Pump Module for Transport 189
10 Parts and Materials for Maintenance 195
Overview of Maintenance Parts 196
Flow Connections 198
Solvent Cabinet Kit 200
Seal Wash Option 201
Pump Heads 202
Purge Valve 206
Cover Parts 208
Leak Parts 210
Fuses 211
Accessory Kit 212
Tools 213
HPLC System Tool Kit 215
11 Identifying Cables 217
Cable Overview 218
Analog cables 220
Remote Cables 222
BCD Cables 225
CAN/LAN Cable 227
RS-232 Cable Kit 228
Agilent 1200 Module to Printer 229

8 Agilent 1290 Infinity Binary Pump User Manual
Contents
12 Hardware Information 231
Firmware Description 232
Electrical Connections 235
Interfaces 237
Setting the 8-bit Configuration Switch 244
Instrument Layout 248
Early Maintenance Feedback 249
13 LAN Configuration 251
What You Have to Do First 252
TCP/IP parameter configuration 253
Configuration Switch 254
Initialization mode selection 255
Dynamic Host Configuration Protocol (DHCP) 257
Link configuration selection 260
Manual Configuration 261
PC and User Interface Software Setup Setup 267
14 Appendix 269
General Safety Information 270
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002-96-EC) 273
Radio Interference 274
Sound Emission 275
Agilent Technologies on Internet 276

9
Agilent 1290 Infinity Binary Pump User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Binary Pump 11
Pump Principle 12
System Overview 15
Leak and Waste Handling 15
This chapter gives an introduction to the pump, instrument overview and
internal connectors.

10 Agilent 1290 Infinity Binary Pump User Manual
1 Introduction
Features
Features
The Binary pump is designed for highest performance, GLP compliance and
easy maintenance. It includes the following features:
•Seal wash for continued high lifetime of pump seals for buffer applications.
•Solvent selection valve for method flexibility.
•Jet Weaver for optimum mixing performance with a minimum of delay
volume.
•Automatic purge function for ease of use and unattended preparation of the
system.
•Auto tuning of the delivery cycle for compensation of elasticity and solvent
volume change effects (compressibility, thermal expansion, mixing
contraction).
•Solvent selection for optimum flow and composition accuracy.
•Fast defill function for improved intake and delivery performance.
•Two pistons in series design for increased reliability.
•High resolution piston movement control for smooth and reliable motion.
For specifications, see Table 2 on page 22.
NOTE
This Binary pump has been introduced together with the Agilent 1290 Infinity Liquid
Chromatograph.

Agilent 1290 Infinity Binary Pump User Manual 11
Introduction
1
Overview of the Binary Pump
Overview of the Binary Pump
The Agilent 1290 Infinity Binary Pump comprises two identical pumps
integrated into one housing. Binary gradients are created by high-pressure
mixing. A degassing unit is included for applications that require best flow
stability, especially at low flow rates, for maximum detector sensitivity. The
flow path of the pump has been optimized for minimal delay of gradients.
Typical applications are high throughput methods with fast gradients on high
resolution 2.1 mm columns. The pump is capable of delivering flow in the
range of 0.05-5 mL/min against up to 1200 bar. A solvent selection valve
allows forming binary mixtures (isocratic or gradient) from one of two
solvents per channel. Active seal wash is available for use with concentrated
buffer solutions.

12 Agilent 1290 Infinity Binary Pump User Manual
1 Introduction
Pump Principle
Pump Principle
The Binary Pump is based on a two-channel, dual-piston in-series design
which comprises all essential functions that a solvent delivery system has to
fulfill. Metering of solvent and delivery to the high-pressure side are
performed by two pump assemblies which can generate pressure up to
1200 bar.
Each channel comprises a pump assembly including independent pump drive
for each piston, pump head, inlet valve, outlet valve, solvent heat exchanger
and an outlet filter. The two channels are fed into a low-volume mixing groove
in an automatic purge valve and a Jet Weaver mixer, with 35or 100 l volume
can be added downstream for optimum mixing performance.
A system pressure sensor, for monitoring the pump pressure, is attached to
the purge valve, normally connected in the B-channel of the pump, before the
mixing groove, in order to minimize delay volumes.

Agilent 1290 Infinity Binary Pump User Manual 13
Introduction
1
Pump Principle
Figure 1 The hydraulic path
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14 Agilent 1290 Infinity Binary Pump User Manual
1 Introduction
Pump Principle
In the user interface the solvent in use for each channel can be selected in
order to get optimum flow and composition accuracy. Although selecting the
right solvent is not required for low ripple, especially for composition accuracy
across pressure it is vital for the compensation algorithm to use the right
solvent properties. The density of the solvents is increased under the
influence of pressure and a certain displaced solvent will expand again when
the pressure is released, for example across the column. In order to achieve
the correct volumetric flow while sample passes through the detector it is
necessary to correct for density related flow inaccuracies in the pump module.
In order to always deliver the best possible pressure stability, the pump
constantly tunes the delivery cycle for elasticity and dead volume effects. With
this feature the pump is able to deliver a stable and accurate flow without
requiring individual calibration settings. A further feature of the control and
compensation algorithm is leak correction. With this it is even possible to
compensate for minor leaks in primary pump chamber (inlet valve and seal),
without the performance of the pump being affected.
To increase the robustness of the pump it uses a fast defill function which
reduces the delivery time of the primary piston, thereby reducing the net
effects of leaks considerably. Furthermore each pump channel has only two
valves on its two pump heads which also reduce the potential of failures. In
order to reduce stresses on the pump hardware, it uses a smooth motion
control, which slowly increases or decreases the speed of the pistons to
prevent shock movements. In order to be able to control these movements a
high resolution encoder unit is attached to the pump drives which resolves a
revolution into 65000 steps, and each step translates to a volume of about
300 pl.

Agilent 1290 Infinity Binary Pump User Manual 15
Introduction
1
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste handling. It
is important that all security concepts are understood and instructions are
carefully followed.
Figure 2 Leak and waste handling concept (overview - typical stack configuration as
an example)
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16 Agilent 1290 Infinity Binary Pump User Manual
1 Introduction
System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent.
The maximum volume for an individual bottle stored in the solvent cabinet
should not exceed 2.5 L. For details, see the usage guideline for the Agilent
1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been
shipped with the solvent cabinet, electronic copies are available on the
Internet).
The leak pan (2) (individually designed in each module) guides solvents to the
front of the module. The concept covers also leakages on internal parts
(e.g. the detector’s flow cell). The leak sensor in the leak pan stops the running
system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module to
the next, as the solvent flows into the next module’s leak funnel (3, B) and the
connected corrugated waste tube (3, C). The corrugated waste tube guides the
solvent to the next lower positioned module’s leak tray and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to waste.
The condense drain outlet of the autosampler cooler (5) guides condensate to
waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

17
Agilent 1290 Infinity Binary Pump User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 18
Physical Specifications 21
Specifications 22
This chapter provides information on environmental requirements, physical
and performance specifications.

18 Agilent 1290 Infinity Binary Pump User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Consideration
The module power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 1 on page 21. Consequently, there is
no voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when
the cover is opened and the module is connected to power.
➔Make sure that it is always possible to access the power plug.
➔Remove the power cable from the instrument before opening the cover.
➔Do not connect the power cable to the Instrument while the covers are removed.
WARNING
Incorrect line voltage at the module
Shock hazard or damage of your instrument can result if the devices are connected
to line voltage higher than specified.
➔Connect your module to the specified line voltage.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

Agilent 1290 Infinity Binary Pump User Manual 19
Site Requirements and Specifications
2
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of
all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that
is suitable for your particular country or region.
WARNING
Unintended use of power cords
Using power cords for unintended purposes can lead to personal injury or damage
of electronic equipment.
➔Never use a power cord other than the one that Agilent shipped with this
instrument.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
WARNING
Electrical shock hazard
Solvents may damage electrical cables.
➔Prevent electrical cables from getting in contact with solvents.
➔Exchange electrical cables after contact with solvents.

20 Agilent 1290 Infinity Binary Pump User Manual
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 21) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
NOTE
Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench
rack. This option helps to save bench space as all modules can be placed into one single
stack. It also allows to easily relocate the instrument to another Lab.
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.