Agilent Technologies 1260 Infinity Quaternary LC VL User manual

Agilent Technologies
Agilent 1260 Infinity
Quaternary LC VL
System Manual and
Quick Reference

Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
Notices
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Manual Part Number
G1311-90310
Edition
06/2010
Printed in Germany
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76337 Waldbronn
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For Research Use Only

Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference 3
In This Book
In This Book
This book describes the Agilent 1260 Infinity Quaternary LC VL
1 Introduction
This chapter gives an introduction to the Agilent 1260 Infinity Quaternary LC
VL, the underlying concepts and the features of the Agilent 1260 Infinity
Quaternary LC VL.
2 Specifications
This chapter provides information about specifications for the LC system.
3 Optimization of the Agilent 1260 Infinity Quaternary LC VL
This chapter considers how to apply the theory and use the features of the LC
system to develop optimized separations.
4 System Setup and Installation
This chapter includes information on software installation, stack
configurations and preparing the system for operation.
5QuickStartGuide
This chapter provides information on data acquisition and data analysis with
the Agilent 1260 Infinity Quaternary LC VL.
6 Appendix
This chapter provides addition information on safety, legal, web and the Edit
Entire Method.

4 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
Contents
Contents
1 Introduction 7
Introduction to the Agilent 1260 Infinity Quaternary LC VL 8
Features of the Agilent 1260 Infinity Quaternary LC VL 10
System Components 11
Optimizing the Stack Configuration 12
Quaternary pump 17
Autosampler 19
Thermostatted column compartment 24
Detector 25
2 Specifications 37
Physical Specifications 38
Performance Specifications 40
3 Optimization of the Agilent 1260 Infinity Quaternary LC VL 49
Optimizing the Pump 50
Optimizing the Autosampler 54
Optimizing the Thermostatted Column Compartment 61
Optimizing the Detector Regarding to the System 62
Optimizing Detection with DAD 63
Optimizing Detection with VWD 78
4 System Setup and Installation 83
Installing Software 84
Installing the Modules 85
5QuickStartGuide 91
Preparing the System 92
Setting Up the Method 97

Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference 5
Contents
6 Appendix 99
Safety 100
The Waste Electrical and Electronic Equipment Directive 103
Lithium Batteries Information 104
Radio Interference 105
Sound Emission 106
Solvent Information 107
UV-Radiation 108
Declaration of Conformity for HOX2 Filter 109
Agilent Technologies on Internet 110

6 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
Contents

7
Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
Agilent Technologies
1
Introduction
Introduction to the Agilent 1260 Infinity Quaternary LC VL 8
Concept of the Quaternary LC VL System 8
System Properties 8
Features of the Agilent 1260 Infinity Quaternary LC VL 10
System Components 11
Optimizing the Stack Configuration 12
One Stack Configuration 12
Two Stack Configuration 15
Quaternary pump 17
Hydraulic Path 18
Autosampler 19
Sequences 21
Thermostatted column compartment 24
Detector 25
Diode-Array Detector (DAD) 25
Variable Wavelength Detector (VWD) 30
This chapter gives an introduction to the Agilent 1260 Infinity Quaternary LC VL,
the underlying concepts and the features of the Agilent 1260 Infinity
Quaternary LC VL.

8 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
1Introduction
Introduction to the Agilent 1260 Infinity Quaternary LC VL
Introduction to the Agilent 1260 Infinity Quaternary LC VL
Concept of the Quaternary LC VL System
The Agilent 1260 Infinity Quaternary LC VL offers the most flexibility for
solvent selection and automation in HPLC method development, research and
all HPLC applications requiring continuous access to a wide range of solvent
choices. The availability to rapidly switch between methods using different
solvents and the capability of using binary, ternary or quaternary solvent
gradients make the Agilent 1260 Infinity Quaternary LC VL the most flexible
system on the market.
System Properties
The Agilent 1260 Infinity Quaternary LC VL is ideally suited for multi-method,
high-throughput workflows. It offers:
•Convenient access to four solvents for isocratic or gradient analysis for
rapid method development and speed up preparation of mobile phases and
flushing the HPLC system.
•Pressure range up to 400 bar.
•A wide flow range up to 10 mL/min and a delay volume of 800 – 1100 µL
supports narrow-bore, standard and semi-preparative applications.
•Easy programming and control through the Instant Pilot G4208A (requires
firmware B.02.08 or above) (p/n G4208-67001) or through an Agilent Data
System.
•Included micro vacuum degasser offers high degassing efficiency for
trouble-free operation and highest performance and completely eliminates
the need for helium sparging.
•Direct front access for quick exchange of maintenance parts.
•Fast problem identification by self-diagnostics, built-in log books and
preprogrammed test methods.

Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference 9
Introduction 1
Introduction to the Agilent 1260 Infinity Quaternary LC VL
•Early maintenance feedback (EMF) that continuously tracks long-term
instrument usage and user-defined limits with feedback message when limit
is exceeded.
•Upgradeability and expandability with the complete range of Agilent 1200
Infinity Series HPLC modules.
•Agilent Data System helps you manage your lab for best chromatographic
quality with intuitive diagnostic and monitor capabilities and alert
functions to notify you of problems.

10 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
1Introduction
Features of the Agilent 1260 Infinity Quaternary LC VL
Features of the Agilent 1260 Infinity Quaternary LC VL
The Agilent 1260 Infinity Quaternary LC VL is designed to offer the greatest
flexibility for performing analytical liquid chromatography using all types of
current and emergent column technologies.
The quaternary system as described in this manual offers:
•Gradients of up to 4 different solvents.
•Pressure range up to 400 bar.
•Sophisticated pump control to deliver very low chromatographic noise and
very low acoustic noise for better results and better working environment.
•Degasser and automatic purge valve integrated into pump module.
•Variable volume autosampler with reduced delay volume, reduced
carryover and the option to operate as a fixed loop autosampler.
•Thermostated column compartment with a pressure range up to 600 bar
•Choice of detectors (a set of different flow cells is available for different
detectors to fit application needs regarding flow ranges (nano scale, micro
scale, standard and preparative applications) and pressures):
•Diode-array detector with greatly enhanced sensitivity and baseline
stability using cartridge cell system with optofluidic waveguides (data
collection rate up to 80 Hz with full spectral information) or
•Variable wavelength detector.