Biotage V-10 Touch User manual

Biotage®V-10 Touch
User Manual

Biotage®V-10 Touch User Manual | © Biotage 2019
Contents
CONTENTS
System Overview
1 Evaporation Methods
1 Vials
2 External Vacuum Pump (Optional)
2 Automation (Optional)
2 Solvent Manager (Optional)
2 Liquid Handler (Optional)
2 Biotage®
V- Touch Control Centre (Optional)
Define Methods, Vials, and System Settings
3 Define a Method
4 Define a Vial
4 Define System Settings
5 Define Sample Pump Settings
5 Reset Parameter Settings
Prepare and Start the System
6 Change Upper Vial Holder
6 Change Carousel Sample Holder
7 Fluid Connections
7 Start the System
8 Defrost and Drain the Condenser
8 Prime the System
9 Set Up the Liquid Handler (Optional)
 Evaporate
10 Set Up and Start an Evaporation Run
11 Monitor an Evaporation Run
11 Edit an Evaporation Run
11 End and Unload an Evaporation Run
11 Stop an Evaporation Run
 Redissolve
12 Set Up and Start a Redissolve Operation
12 Stop a Redissolve Operation
 Evaporate Using Biotage
®
V- Touch Control Centre
13 Connect an External Computer
13 Prepare the System
13 Define Biotage®V- Touch Control Centre Settings
13 Clean the Needle and Vial Tubing
14 Set Up an Evaporation Job
14 Modify or Delete a Queued Evaporation Job
15 Save and Open a Job List
15 Start the Queue
16 Monitor an Evaporation Job
16 Edit an Evaporation Job in Progress
Biotage®V- Touch
User Manual
16 Unload and Clear an Evaporation Job
16 Pause an Evaporation Job
16 Stop an Evaporation Job
16 Log Files
 Define Workspaces for a Liquid Handler
17 Create and Edit a Rack
17 Create and Edit a Workspace
18 Verify the Setup of a Workspace
 Maintenance
20 Clean the Exterior of the System
20 Clean the Condenser Pot
20 Clean the Lower Vial Holder
21 Clean the Plastic Guard
21 Clean or Replace the Vial Face Seal and
Lubricate or Replace the Vial Holder O-rings
22 Clean the Upper Spin Head, Vapor Duct,
and Condenser Pot Inlet Region
22 Defrost the Condenser
22 Drain the Condenser
22 Replace the Drain Tube
22 Replace the Fuses
23 Clean the Tubing
23 Replace the Tubing
24 Liquid Handler Maintenance
 Hints and Tips
25 Methods for Various Solvents
 Troubleshooting
26 Condenser
26 Vacuum
26 Carousel
27 Sample Pump
 General Information
28 Accessories and Spare Parts
28 Manufacturer
28 Contact Us


System Overview
System Overview
Biotage®V- Touch is a solvent evaporator system for
applications in organic and inorganic chemistry. The evaporator
is operated via its touch screen or V- Touch Control Centre.
The system enables evaporation of single samples (up to 12 mL)
or large pooled volumes using a built-in sample pump (optional).
Adding a vial carousel enables unattended evaporation of up to
16 samples.
By connecting a liquid handler, multiple fractions from different
test tubes and racks can be combined and dried in a single vial
in one operation. This requires the built-in sample pump and
V- Touch Control Centre; see page 2.
Figure 1
B
C
J
DK
F
E
A
I
M
N
G
H
L
.The front side of Biotage®V-10 Touch without a carousel or
built-in sample pump. A = ventilation panel, B = touch screen, C = upper
vial holder, D = IR sensor, E = sample heater, F = plastic guard, G= lower
vial holder, H = vial loading arm, I = connection for external vacuum pump,
J = condenser pot clamp, K = vacuum insulated condenser pot, L = drain
valve, M = pump exhaust connector, and N = pump exhaust catchpot.
Evaporation Methods
The system comes with eight predefined methods that cover the
majority of evaporation requirements; see Table 1. You can also
define your own methods; see page 3.
The evaporation method should be selected based on the
solvent with the highest atmospheric boiling point (BP). For a
list of solvents and their boiling points, see page 25.
Method Description Boiling Point (BP)
Very High Boil Very high boiling point. > 160°C
High Boil High boiling point. 110°C ≤ BP ≤ 160°C
Aqueous 90°C ≤ BP ≤ 110°C
Mixed Vol & HBP Mixed volatile and high
boiling point.
55°C ≤ BP ≤ 160°C
HPLC Fractions
Fractions from reverse
phase Flash and prep LC.
55°C ≤ BP ≤ 110°C
Volatile 55°C ≤ BP ≤ 90°C
Mixed Volatile Fractions from Flash
chromatography.
55°C ≤ BP ≤ 90°C
High Volatile Highly volatile. < 55°C
Table 1.The eight predefined Biotage methods.
Vials
Samples can be dried in vials of volumes 4, 8, 16, 20, and 30 mL.
The 20 and 30 mL vials are the standard vials. 4, 8, and 16 mL
vials require vial adapters (see Figure 2) and, if a carousel is used,
different carousel sample holders (see page 6). Use the vial
with the most appropriate volume for your sample; see Table 2.
Total Vial
Volume (mL)
Maximum Fill
Volume (mL)
Needs Upper
Vial Adapter
Needs Lower
Vial Adapter
30 12 - -
20 8 - -
16 8 - Yes
84Yes Yes
42Yes Yes
Table 2.The maximum fill volume and adapter requirements for the five
predefined Biotage vials.
Figure 2.Upper and lower vial adapters.

Biotage®V-10 Touch User Manual | © Biotage 2019
System Overview
External Vacuum Pump (Optional)
The system has a built-in vacuum pump. It is also possible
to connect an external vacuum pump. An external vacuum
pump is recommended to reach the low pressure needed
for the evaporation methods Very High Boil, High Boil, and
Mixed Vol & HBP.
Automation (Optional)
Adding the vial carousel enables unattended evaporation of up
to 16 samples.
Carousel Control Box
The carousel status is indicated by four LEDs located on the
carousel control box behind the carousel; see A in Figure 3.
LED Description
1A green light is lit when the carousel is turned on.
2The light is turned off when no carousel sample holder is
mounted.
A yellow light is lit when a carousel sample holder is
mounted but not locked.
A green light is lit when the carousel sample holder is in a
locked position.
3A yellow light is blinking when the carousel is busy.
4A red light is lit when a carousel error has occurred. See the
error dialog that opens on the touch screen.
Figure 3
A
B
C
D
E
G
F
. Biotage®V-10 Touch with a carousel and a built-in sample pump.
A = carousel control box with four LEDs, B = carousel sample holder,
C = carousel carriage, D = pump manifold, E = sample loop, F = system
solvent bottle, and G = external sample flask.
Solvent Manager (Optional)
If the system is equipped with a built-in sample pump, the
following features are enabled:
» Evaporation of large volumes. Multiple
injections from a single sample.
» Solvent replacement. Samples can be
automatically redissolved after evaporation.
» Combining fractions. Multiple injections from multiple
samples; see "Liquid Handler (Optional)" below.
Liquid Handler (Optional)
By connecting a liquid handler (Gilson GX-271, see Figure 4),
multiple fractions from different test tubes and racks can
be combined and dried in a single vial in one operation. This
requires the built-in sample pump and V- Touch Control Centre.
Figure 4.Gilson GX-271 with Isolera™racks. Chromatography fractions can
be combined and concentrated directly from the chromatography system
rack without manual intervention.
Biotage® V- Touch Control Centre (Optional)
V- Touch Control Centre can be used to control any V- Touch
system. The software is installed on an external computer and
adds the capabilities of integrating the V- Touch system with
a liquid handler as well as running the carousel with different
methods for different carousel positions.
V- Touch Control Centre consists of two application programs:
» Controller. Use this application to control runs
on the V- Touch system; see page 13.
» Editor. Use this application to create and modify
workspaces that allow racks to be loaded onto
the liquid handler bed; see page 17.


Define Methods, Vials, and System Settings
The system comes with eight predefined methods (see Table 3)
and five predefined vials (see Table 2 on page 1). The
predefined methods, vials, and other system settings have
been set to provide optimal evaporation of most solvents.
You can also define your own methods and vial s by modifying the
methods and vials named Custom 1, Custom 2, and Custom 3.
When using a carousel, a liquid handler, and/or an external
vacuum pump, they must be enabled in the system settings (see
"Define System Settings" on page 4). Note that even though
it is possible to use a liquid handler without enabling it in the
system, it must be enabled to prevent users from performing
tasks from the touch screen that involve the sample inlet tube.
To reset settings to factory default, see "Reset Parameter
Settings" on page 5.
Note: To define V- Touch Control Centre settings, see page 13.
Define a Method
Enter the method view (see Figure 7) by pressing Configuration
in the menu bar (see Figure 5) and then Edit Methods (see
Figure 6). Select the method that you want to edit. The following
method parameters are available:
» Target Temperature: The target and maximum
allowed vial temperature (in °C).
» Max Evap Power: The maximum heating power
(in percentages) generated by the vial heater.
» Start Heating Temp: If the regulator conditions for starting
the heater have not yet been fulfilled when the vial
temperature is below this value (in °C), the heater is started.
» Target Pressure: The target pressure (in mbar)
for the evaporation.
Redissolve System Ready
Last Run: 7:35
Sample Pump
CongurationMaintenanceEvaporate
Factory Reset
Edit Methods
Edit Vials
System Setup
Legal Info
Software Ver
Figure 5.The Configuration view.
MaintenanceRedissolve System Ready
Last Run: 7:35
Evaporate
Very High Boil Mixed Volatile
Aqueous Custom 1
Volatile Custom 3
High Boil Mixed Vol & HBP
HPLC Fractions Custom 2
High Volatile
Conguration
Back Edit Methods
Figure 6.The Edit Methods view.
Define Methods, Vials, and System Settings
Parameter Very High
Boil High Boil Volatile HPLC Fractions Aqueous Mixed Volatile
High
Volatile
Mixed Vol
& HBP
Target Temperature (°C) 56 48 36 46 46 36 36 48
Max Evap Power (%) 100 100 100 100 100 50 50 50
Start Heating Temp (°C) 15 15 5515 555
Target Pressure (mbar) 0030 30 830 100 30
Vac Pump Speed 1 (mbar/s) 150 (Max) 150 (Max) 50 (Med) 10 0 (High) 150 (Max) 15 (Low) 50 (Med) 50 (Med)
Vac Pump Speed 2 (mbar/s) 25 (Max) 25 (Max) 2 (Low) 5 (Med) 10 (High) 2 (Low) 2 (Low) 2 (Low)
Rotational Speed (rpm) 6000 6000 6000 6000 6000 7000 6000 7000
End of Evaporation Auto Auto Auto Auto Auto Auto Auto Auto
Min Evap Time (min) 22111115
Max Evap Time (min) 20 20 10 20 20 10 10 20
Final Dry (min) 33233223
Use Ext Vac Pump YES YES NO NO NO NO NO YES
Table 3.The default settings of the eight predefined Biotage methods.

Biotage®V-10 Touch User Manual | © Biotage 2019
Define Methods, Vials, and System Settings
» Final Dry: The drying time (in minutes) for
finalizing the run at lowest possible pressure.
» Use Ext Vac Pump: Defines whether an external vacuum
pump (if enabled in the System Setup view) is to be
used (YES) or not (NO). An external vacuum pump
is recommended to reach the low pressure needed
when using the following predefined methods:
» Very high boiling point (Very High Boil)
» High boiling point (High Boil)
» Mixed volatile and high boiling point (Mixed Vol & HBP)
Define a Vial
Enter the vial view (see Figure 8) by pressing Configuration in
the menu bar and then Edit Vials. Select the vial that you want
to edit. The following vial parameters are available:
» Dispense Volume: The volume (in mL) that may be dispensed
into the vial. It is important that this value does not exceed
the maximum fill volume;
see Table 2 on page 1.
» Dispense Rate*: The flow rate (in mL/min) when
dispensing sample solution from the sample loop
into the vial, using the built-in sample pump.
Note: When using V- Touch Control Centre, the Sample
Dispense Rate (mL/min) parameter (see page 13)
overrides this parameter.
Define System Settings
Enter the System Setup view (see Figure 9) by pressing
Configuration in the menu bar and then System Setup. The
following system parameters are available:
» Redissolve Speed*: The rotational speed of the
vial (in rpm) when a sample is automatically
redissolved after evaporation.
Note: When using V- Touch Control Centre, the Speed (rpm)
parameter (see page 14) overrides this parameter.
» Vac Pump Speed 1: The pressure drop (in mbar/s)
when the pressure is above or equal to 150 mbar.
Possible settings are:
» Low – 15 mbar/s
» Med – 50 mbar/s
» High – 100 mbar/s
» Max – 150 mbar/s
» Vac Pump Speed 2: The pressure drop (in mbar/s) when
the pressure is below 150 mbar. Possible settings are:
» Low – 2 mbar/s
» Med – 5 mbar/s
» High – 10 mbar/s
» Max – 25 mbar/s
» Rotational Speed: The rotational speed of the
vial (in rpm) during the evaporation.
» End of Evaporation: This parameter controls when
the evaporation is ended. Possible values are:
» Auto: The evaporation is ended
automatically when the vial is dry.
» Continual: The evaporation is ended
manually by pressing Stop.
» Timed: The evaporation is ended when
the Max Evap Time is reached.
Note: If using a liquid handler, Auto or Timed must be
selected.
» Min Evap Time: If End of Evaporation is set to
Auto, this is the minimum time (in minutes) for an
evaporation before auto dry detection starts.
» Max Evap Time: If End of Evaporation is set to:
» Auto, this is the maximum time
(in minutes) for an evaporation.
» Timed, this is the total time (in minutes)
for an evaporation.
MaintenanceRedissolve Conguration System Ready
Last Run: 7:35
Target Temperature
Use Ext Vac Pump
Rotational Speed
 °C
Start Heating Temp  °C
Vac Pump Speed : Max : Hi
Min/Max Evap Time  min  min
End of Evaporation Auto
Target Pressure  mbar
Max Evap Power  
Final Dry  min
 rpm
Evaporate Conguration
Custom 1
Back Edit Methods >
NO YES
Figure 7.Use the methods Custom 1, Custom 2, and Custom 3 to define
your own methods.
MaintenanceRedissolve System Ready
Last Run: 7:35
Dispense Volume
Dispense Rate
4 mL
8 mL/min
Custom 1
Evaporate Conguration
Edit Vials >
Back
Figure 8.Use the vials Custom 1, Custom 2, and Custom 3 to define your
own vials.


Define Methods, Vials, and System Settings
» Redissolve Cycles*: The number of spin cycles when a
sample is automatically redissolved after evaporation.
Note: When using V- Touch Control Centre, the Cycles
parameter (see page 14) overrides this parameter.
» Date: The current date.
» Time: The current time.
» IP Address: The system's IP address is used to
communicate with an external computer running V-
Touch Control Centre. It is recommended that the default
IP address is used. For information on how to connect
an external computer, see the “Biotage®V- Touch
Installation and Safety” document (P/N 414661).
» Brightness: The brightness of the
touch screen (in percentages).
» Liquid Handler*: Defines whether a liquid handler is
connected (YES) or not (NO). When set to YES, it is not
possible to perform tasks from the touch screen that
involve the sample inlet tube i.e. set the evaporation
mode to External Flask and perform a prime.
» Carousel†: Defines whether the carousel
can be used (YES) or not (NO).
» Ext Vacuum Pump: Defines whether an external
vacuum pump is connected (YES) or not (NO).
» Ext Vacuum Trig Point: If the external vacuum pump
is enabled in the method, it will be activated when
the pressure reaches this value. This field is only
enabled when the Ext Vacuum Pump is set to YES.
Define Sample Pump Settings
If the system is equipped with a built-in sample pump, enter the
Sample Pump view (see Figure 10) by pressing Configuration
in the menu bar and then Sample Pump*. The following sample
pump parameters are available:
» Prime Aspiration Rate: The aspiration rate (in mL/min)
when priming the tubing with system solvent.
» Prime Dispense Rate: The dispense rate (in mL/min)
when priming the tubing with system solvent.
Note: The higher the prime rate, the more efficient the
removal of air bubbles will be.
» Sample Aspiration: The flow rate (in mL/min) when
aspirating sample solution into the sample loop.
Note: The default rate (10 mL/min) should work for most
solvents. However, we suggest a lower aspiration rate for
solvents with high viscosity or low boiling points.
» Sample Tube Volume: The volume of the probe and sample
inlet tube (in mL). This volume is taken into account when
aspirating sample solution from an external flask into
the sample loop. When using the probe and sample inlet
tube supplied by Biotage, it must be set to 1.2 mL.
Note: The sample pump settings above are not used when using
V- Touch Control Centre. To define V- Touch Control Centre
settings, see page 13.
Reset Parameter Settings
To reset the settings in the System Setup view (see Figure 9) or for
a single predefined method or vial to the factory defaults, press
Reset to Default in the applicable view and then Yes to confirm.
To reset all settings in the Configuration view (see
Figure 5 on page 3), except those of the methods and vials
named Custom 1, Custom 2, and Custom 3, and the date and
time, to the factory defaults:
1. Select Configuration in the menu bar.
2. Press Factory Reset and then Yes to confirm.
Note that the carousel, liquid handler, and external vacuum
pump features are disabled by default.
*Only enabled if the system is equipped with a built-in sample pump.
†Only enabled if the system is equipped with a carousel.
MaintenanceRedissolve System Ready
Last Run: 7:35
System Setup
Evaporate Conguration
Back
Reset to Default
Liquid Handler
Carousel
Ext Vac Pump NO YES
Ext Vac Trig Point  mbar
Redissolve Speed
Redissolve Cycles
 rpm
 cycles
Date
Time
IP Address
2016Mar18
  
NO YES
NO YES
 
:
Brightness  
NO YES
NO YES
Figure 9.The System Setup view.
MaintenanceRedissolve System Ready
Last Run: 7:35
CongurationEvaporate
Prime Dispense Rate
Prime Aspiration Rate
Sample Aspiration 10 mL/min
100 mL/min
100 mL/min
Back Sample Pump
Reset to Default
Sample Tube Volume 1.2 mL
Figure 10. The Sample Pump view.

Biotage®V-10 Touch User Manual | © Biotage 2019
Prepare and Start the System
Warning
» Observe general as well as specific safety regulations for the
use of the equipment at all times; see the “Biotage®V-10 Touch
Installation and Safety” document (P/N 414661).
Change Upper Vial Holder
Warning
» Ensure that the system is turned off and the power cord is
disconnected.
» The heater nozzle may get warm during operation.
» Never operate the system with the plastic guard removed.
Ensure that the correct upper vial holder is used on the system;
see Figure 11.
Figure 11
AB
.A: Upper vial holder for 4, 8, 20, and 30 mL vials. B: Upper vial
holder for 16 mL vials.
1. Turn off (O) the system and disconnect the power cord.
2. Remove the plastic guard by loosening the four mounting
screws using the T20 Torx screwdriver supplied with the
system; see Figure 12A.
3. Remove the heater nozzle by pulling it straight out; see
Figure 12B.
4. Replace the upper vial holder by pulling it down firmly (see
Figure 12C) until it is completely free of its mounting.
5. Put the heater nozzle and the plastic guard back in place.
Figure 12
A B C
.Removing the plastic guard (A), nozzle (B), and upper vial
holder (C).
Change Carousel Sample Holder
There are four different carousel sample holders available:
20 and 30 mL vials (P/N 411181), 16 mL vials (P/N 411985), 4 mL
vials type A and B (P/N 411182), and 4 mL vials type C (P/N 411183).
Note: Ensure that the system is idle before removing or
mounting a carousel sample holder.
Remove the Carousel Sample Holder
Release the carousel sample holder by turning it
counterclockwise until it stops and then lift it straight up.
Mount the Carousel Sample Holder
1. Insert the two front pegs on the bottom of the carousel sample
holder into the corresponding holes in the carousel carriage
(see Figure 13) and let the sample holder fall into place.
Figure 13.Aligning the front pegs with the holes on the carousel carriage.
2. If the carousel sample holder does not fall into place, rotate
the vial holders until the driving wheel peg on the carousel
carriage slides into the hole on the bottom of the sample
holder. See Figure 14.
Figure 14.
A B C
Rotate the vial holders (A) until the driving wheel peg (B) slides
into the hole on the bottom of the carousel sample holder (C).
3. Lock the carousel sample holder by turning it clockwise
until it stops. When the system is on, correct mounting is
indicated by the second LED from the top on the carousel
control box being green.
Prepare and Start the System


Prepare and Start the System
Fluid Connections
If the system is equipped with a built-in sample pump, all fluid
connections should be connected as shown in Figure 15. Note
that the sample inlet tube (blue in Figure 15) can be connected
to either an external flask or a liquid handler.
Figure 15.The fluid connections (not to scale).
The following ports are available on the pump manifold:
Port Connect
LOOP (x2) The sample loop (red in Figure 15).
SAMPLE
ROBOT
The sample inlet tube (blue in Figure 15). The system
is shipped with a sample inlet tube that must be used
when aspirating sample solution from an external
flask. If using a liquid handler, use the sample inlet
tube that is supplied with the liquid handler.
Note: During a prime operation that is started from
the touch screen, the sample inlet tube must be
placed in a suitable waste container.
VIAL The vial tubing that is connected to the sample inlet
connection (green in Figure 15). The green vial tube
must stick out 2 to 3 mm from the upper vial holder
or the upper vial adapter; see Figure 16.
SS The system solvent inlet tube (orange in Figure 15).
Adjust the Green Vial Tube
The height of the green vial tube has to be adjusted according to
the vial being used:
» Vials without upper vial adapter: The tube must stick out
2 to 3 mm from the upper vial holder; see Figure 16A.
» Vials with upper vial adapter: The tube must stick out
2 to 3 mm from the upper vial adapter; see Figure 16B.
Adjust the height by loosening the upper fitting at the sample
inlet connection; see B in Figure 37 on page 23.
Figure 16
A B
.The tube must stick out 2 to 3 mm from the upper vial holder (A)
or the upper vial adapter (B).
Start the System
Warning
» If the system is equipped with a carousel, keep your hands away
from the carousel during initialization.
1. Turn on (l) the system using the mains switch on the left side
of the system.
2. If applicable, ensure that the external vacuum pump is
connected and turned on during the evaporation operation.
Note: The external vacuum pump will only be used if
it has been enabled both in the System Setup view
(see "Define System Settings" on page 4) and in the
method (see "Define a Method" on page 3).
3. If the system is equipped with a carousel, the Alert –
Initializing Carousel dialog opens on the touch screen.
Press Initialize Carousel and keep your hands away from the
carousel until it has stopped moving.
Note: The carousel can only be used if it has been enabled
in the System Setup view; see "Define System Settings" on
page 4.
4. When the Confirm Collection Vessel dialog opens on the
touch screen, ensure that the lower end of the drain tube is
inserted into a collection vessel of a suitable size, and that
the tube cannot become immersed in the waste liquid.

Biotage®V-10 Touch User Manual | © Biotage 2019
Prepare and Start the System
5. Press Tube and Vessel in Position to start the drain
operation and to perform the condenser start-up.
6. Please wait for the condenser to reach its target temperature
(-25 °C). This can take up to 20 minutes.
Note: If the system is used before the condenser temperature is
below -20°C, the evaporation performance will be compromised.
If the condenser is already cold, i.e. the system has recently
been operated, the condenser start-up step is skipped.
Defrost and Drain the Condenser
As the condenser accumulates ice it becomes less efficient.
If ice has built up, a defrost operation must be performed.
See instructions on page 22. After the defrost operation is
completed, the condenser will be drained.
The condenser is automatically drained when the system
is started, after each evaporation, and after each defrost
operation. It is also possible to drain the condenser manually;
see instructions on page 22.
When using V- Touch Control Centre, it is possible to
automatically defrost the condenser after a certain amount of
sample solution has been evaporated using the liquid handler.
If enabled, the automatic defrost will be performed after a job is
completed and not in the middle of a job. See "Define Biotage®
V- Touch Control Centre Settings" on page 13.
Prime the System
Warning
» Handle chemical and liquid waste according to the Safety Data
Sheets and to local/national guidelines on laboratory safety
procedures.
If the system is equipped with a built-in sample pump, use the
prime function to 1) remove any air bubbles from the pump
and tubing by flushing them with system solvent or 2) empty
the pump and tubing of system solvent used in the previous
operation and fill them with a new system solvent.
Note: If a liquid handler is connected to the system, see "Prime
Using Biotage®V- Touch Control Centre" below.
Note: If you need to abort a prime operation, e.g. in case of
leakage, switch off (O) the system using the mains switch on the
left side of the system.
1. If necessary, clean the tubing that is connected to the
VIAL port on the pump manifold as described on page 23
before priming.
2. Insert the system solvent inlet tube into a suitable system
solvent bottle.
Note: The system solvent should be miscible with the
previous sample’s system solvent and compatible with the
next sample solution to be evaporated.
3. Insert the sample inlet tube, which is connected to the
SAMPLE ROBOT port on the pump manifold, into a clean and
empty waste container.
4. Select Maintenance from the menu bar on the touch screen.
5. Press Prime.
6. Press the Prime Volume field and enter the amount of
system solvent to be used. 45 mL is the recommended
minimum volume.
Note: The prime rate is defined in the Sample Pump view;
see "Define Sample Pump Settings" on page 5.
7. Press Prime and then Tube and Waste Container in Position
to start the prime operation.
8. When the prime operation is completed, the text “System
Ready” is displayed in the top right corner of the touch
screen. Remove and empty the waste container.
Prime Using Biotage®V- Touch Control Centre
1. If necessary, clean the tubing that is connected to the VIAL
port on the pump manifold as described on page 23
before priming.
2. In the Controller application, select Settings... from the File
menu. The Settings dialog opens.
3. Set the Needle Drain Volume to 40 mL and the Needle Wash
Volume to 5 mL.
4. Insert the system solvent inlet tube into a suitable system
solvent bottle.
Note: The system solvent should be miscible with the
previous sample’s system solvent and compatible with the
next sample solution to be evaporated.
5. Click .
6. When the prime operation is completed, the text “Completed”
is displayed in the top right corner of the software. If
necessary, remove and empty the waste container.
7. Change the Needle Drain Volume and Needle Wash Volume
parameters back to the desired settings for when cleaning
the needle. Recommended volumes are 10 and 5 mL.


Prepare and Start the System
Set Up the Liquid Handler (Optional)
Before running an evaporation job using a liquid handler, ensure
that the liquid handler is correctly set up.
1. Ensure that the liquid handler has the Z-arm positioned at
175 mm. To adjust the height, loosen the two screws holding
the arm in position using a 2.5 mm Allen wrench. See Figure 17.
Figure 17
AB
.Ensure that the Z-arm is positioned at 175 mm (A). Adjust by
loosening the two screws holding the arm in position (B).
2. Ensure that the needle is flush with the bottom of the needle
guide (see Figure 18A) and not bent. To adjust the needle
height, loosen the needle holder screw (see Figure 18B).
Figure 18.
AB
Ensure that the needle is flush with the bottom of the needle
guide (A). Adjust by loosening the needle holder screw (B).

 Biotage®V-10 Touch User Manual | © Biotage 2019
Evaporate
Evaporate
MaintenanceRedissolveEvaporate Conguration System Ready
Last Run: 7:35
Target Temperature
Target Pressure
46 °C
8 mbar
Method
Current Temperature
Current Pressure
Current Power
Vial Size 20 mL
Aqueous
Evaporation Mode
Evaporate
23.1 °C
1028.0 mbar
0.0 %
Redissolve After
Redissolve Volume
Carousel Mode
NO YES
Total Volume
Remaining Volume ---
Figure 19.The Evaporate view during setup. This system has neither a
built-in sample pump nor a carousel.
Warning
» Keep your hands away from the vial loading arm and carousel
(if used) while the vial is being loaded or unloaded.
Note: To evaporate using V- Touch Control Centre, see page 13.
Set Up and Start an Evaporation Run
Note: The carousel and the built-in sample pump are optional.
1. Prepare the system as described in "Prepare and Start the
System" on page 6.
2. Depending on whether the built-in sample pump is used or
not, insert the sample inlet tube into the sample solution or
dispense the sample solution into the vial or vials (if using the
carousel). Ensure that the dispensed volume does not exceed
the maximum fill volume of the vial; see Table 2 on page 1.
3. Ensure that the correct upper and lower vial adapters are
used, if applicable. See Table 2 on page 1.
4. Depending on whether the carousel is used or not, put the
vial(s) into the carousel sample holder or put the vial into the
lower vial holder.
To put vials into all carousel positions, remove the carousel
sample holder, load the vials, and then remount the sample
holder. See instructions on page 6.
5. Select Evaporate from the menu bar and set up the run;
see Figure 19. For more information, see "Evaporation
Parameters" below.
6. To start the run, press Evaporate.
7. If the Confirm Fill Volume dialog opens, confirm that the
maximum fill volume has not been exceeded (see step 2) by
pressing Fill Volume is Correct.
Evaporation Parameters
Note: Changes made in the Evaporate view will not be saved in
the selected method.
The following evaporation parameters are available:
» Method: The evaporation method. For more information,
see "Evaporation Methods" on page 1.
» Vial Size: The selected vial size; see "Vials" on page 1.
» Target Temperature: The target temperature
defined in the selected method.
» Target Pressure: The target pressure
defined in the selected method.
» Evaporation Mode*:The selected evaporation mode:
» Prefilled Vials: Manual load with prefilled vials.
» External Flask: Multiple injections from a single
sample, using the built-in sample pump. This
feature is disabled when a liquid handler is enabled;
see "Define System Settings" on page 4.
» Total Volume*:The total volume of the sample solution
to be evaporated. If the total volume is larger than
the dispensation volume for the selected vial (see
"Define a Vial" on page 4), the sample solution will
be evaporated in aliquots. This field is only enabled
when Evaporation Mode is set to External Flask.
Note: The volume of the sample inlet tube (+ probe) is taken
into account when aspirating sample solution into the sample
loop (see "Define Sample Pump Settings" on page 5).
Note: To clean the tubing, 2.8 mL of system solvent is added
to the vial when all the sample solution has been aspirated.
MaintenanceRedissolveEvaporate Conguration System Ready
Last Run: 7:35
Target Temperature
Target Pressure
 °C
 mbar
Method
Current Temperature
Current Pressure
Current Power
Vial Size 20 mL
Aqueous
Evaporation Mode Prelled Vials
Evaporate
23.1 °C
1028.0 mbar
0.0 %
Redissolve After
Redissolve Volume 2.0 mL
Carousel Mode All Vials
NO YES
Total Volume
Remaining Volume ---
NO YES
Figure 20.The Evaporate view during setup. This system has both a built-
in sample pump and a carousel.


Evaporate
» Redissolve After*: Defines whether the
sample will be automatically redissolved
after the evaporation (YES) or not (NO).
» Redissolve Volume*: The amount of system solvent
to be used to redissolve the sample. This field is only
enabled when Redissolve After is set to YES.
Note: The redissolve speed and the number of spin cycles is
defined in the Sample Pump view; see "Define Sample Pump
Settings" on page 5.
» Carousel Mode†: The selected carousel mode:
» Not Used: Vials are placed manually
into the lower vial holder.
» All Vials: The carousel will move the vials, one at the
time, to the vial lift until all vial positions have been
processed. Empty positions will be skipped over.
» Continual: The carousel will move the vials, one at
the time, to the vial lift continually until you press
Stop. The evaporation operation will not stop
automatically when all vial positions have been
processed. Empty positions will be skipped over.
*Only enabled if the system is equipped with a built-in sample pump.
†Only enabled if the system is equipped with a carousel.
Monitor an Evaporation Run
The current vial temperature, vacuum pressure, and applied
heater power can be monitored in the Evaporate view;
see Figure 21.
The task in progress is displayed in the top right corner of the
touch screen.
Edit an Evaporation Run
During the evaporation run, it is possible to increase the
target temperature and/or decrease the target pressure in the
Evaporate view. To change these values, simply press the Target
Temperature field and/or the Target Pressure field.
End and Unload an Evaporation Run
If the evaporation run is not ended automatically (the End of
Evaporation is set to Continual in the used method or Carousel
Mode is set to Continual), end the run by pressing Stop.
When the evaporation run is completed, the text “System
Ready” followed by the total run time is displayed in the top
right corner of the touch screen.
The vial will be unloaded when the pressure has been equalized.
Remove the vial(s) and, if necessary, defrost the condenser
(see page 22) and empty the drain collection vessel. The
condenser is automatically drained after each evaporation.
If the sample solution was dispensed from an external flask using
the built-in sample pump, clean the outside of the sample probe
(connected to the sample inlet tube) and then clean the sample
inlet tube and the inside of the probe by priming with 10 mL of a
suitable solvent (see "Prime the System" on page 8).
Stop an Evaporation Run
To abort an evaporation run, at any time, press Stop. Any sample
solution that has been aspirated but not dispensed into the vial
will be returned to the external flask. Note that the vial will not
be unloaded until the pump operation in progress (if any) has
been completed and the pressure has been equalized.
To abort a pump operation, e.g. in case of leakage, switch off (O)
the system using the mains switch on the left side of the system.
Evaporate
Evaporation Mode Prelled Vials
Redissolve After
Redissolve Volume 2.0 mL
Carousel Mode All Vials
NO YES
Total Volume
Remaining Volume ---
MaintenanceRedissolve Conguration Evaporating...
Elapsed Time: 4:43
Target Temperature
Target Pressure
 °C
 mbar
Method
Current Temperature
Current Pressure
Current Power
Vial Size 20 mL
Aqueous
Stop
43 °C
8.1 mbar
53.0%
Figure 21.The Evaporate view during a run.

 Biotage®V-10 Touch User Manual | © Biotage 2019
Redissolve
Redissolve
Set Up and Start a Redissolve Operation
In addition to the possibility of having a sample automatically
redissolved after evaporation, it is possible to manually
redissolve a sample at any time.
Warning
» Keep your hands away from the vial loading arm and carousel
(if used) while the vial is being loaded or unloaded.
Note: The carousel and the built-in sample pump are optional.
1. Prepare the system as described in "Prepare and Start the
System" on page 6.
2. If not using the built-in sample pump, dispense the solvent
into the vial or vials (if using the carousel). Ensure that the
dispensed volume does not exceed the maximum fill volume
of the vial; see Table 2 on page 1.
3. Ensure that the correct upper and lower vial adapters are
used, if applicable. See Table 2 on page 1.
4. Depending on whether the carousel is used or not, put the
vial(s) into the carousel sample holder or put the vial into the
lower vial holder.
To put vials into all carousel positions, remove the carousel
sample holder, load the vials, and then remount the sample
holder. See instructions on page 6.
5. Select Redissolve from the menu bar and set up the
redissolve operation; see Figure 22. For more information,
see "Redissolve Parameters" below.
6. To start, press Redissolve.
7. If the Confirm Fill Volume dialog opens, confirm that the
maximum fill volume has not been exceeded (see step 2) by
pressing Fill Volume is Correct.
8. When the redissolve operation is completed, the text
“System Ready” is displayed in the top right corner of the
touch screen. Remove the vial(s).
Redissolve Parameters
The following redissolve parameters are available:
» Redissolve Speed: The rotational speed of the
vial (in rpm) during the redissolve operation.
» Redissolve Cycles: The number of spin cycles.
» Vial Size: The selected vial size.
» Dispense Method*:The selected dispense method:
» Auto: Solvent is added using the built-in sample pump.
» Manual: Solvent is added manually.
» Redissolve Volume*: The amount of system solvent
to be used to redissolve the sample. This field is only
enabled when Dispense Method is set to Auto.
» Carousel Mode†: The selected carousel mode:
» All Vials: The carousel will move the vials, one at the
time, to the vial lift until all vial positions have been
processed. Empty positions will be skipped over.
» Not Used: Vials are placed manually into the lower vial
holder.
*Only enabled if the system is equipped with a built-in sample pump.
†Only enabled if the system is equipped with a carousel.
Stop a Redissolve Operation
To abort a redissolve operation, press Stop. Note that the vial
will not be unloaded until the pump operation in progress (if any)
has been completed and the pressure has been equalized.
To abort a pump operation, e.g. in case of leakage, switch off (O)
the system using the mains switch on the left side of the system.
Maintenance Conguration System Ready
Last Run: 7:35
Evaporate Redissolve
Redissolve Speed
Redissolve Cycles
Dispense Method
Redissolve
5 cycles
4000 rpm
Auto
Vial Size  mL
Carousel Mode Not Used
Redissolve Volume . mL
Figure 22. The Redissolve view during setup. This system has both a built-
in sample pump and a carousel.


Evaporate Using Biotage®V- Touch Control Centre
Warning
» The system can be controlled either through the touch screen
or through an external computer running V-10 Touch Control
Centre. When using V-10 Touch Control Centre, it is your
responsibility to ensure that you will not interfere with an
operation that is about to be started through the touch screen.
V- Touch Control Centre is installed on an external computer
and adds the capabilities of integrating the V- Touch system
with a liquid handler as well as running the carousel with
different methods for different carousel positions.
V- Touch Control Centre consists of two application programs,
Controller and Editor. Use the Controller to control runs on the
V- Touch system and the Editor to define workspaces for the
liquid handler bed (see page 17).
Note: Never run the Controller and Editor simultaneously as only
one of them will be able to communicate with the system.
Connect an External Computer
For information on how to connect the V- Touch system to an
external computer running V- Touch Control Centre, see the
“Biotage®V- Touch Installation and Safety” document.
Prepare the System
Prepare the system as described in "Prepare and Start the
System" on page 6.
Define Biotage®V- Touch
Control Centre Settings
To change V- Touch Control Centre settings, open the
Controller application and select Settings... from the File menu.
The following parameters are available in the Settings dialog:
» Needle Drain Volume (mL): The amount of
system solvent that is used to clean the tubing
at the drain station before cleaning the needle.
Recommended minimum volume is 10 mL.
» Needle Wash Volume (mL): The amount of system solvent
that is used to clean the inside and outside of the needle at
the wash station. Recommended minimum volume is 5 mL.
» Needle and Sample Inlet Tube Volume (mL): The volume
of the needle and sample inlet tube. This volume is
taken into account when aspirating sample solution
from a test tube on the liquid handler bed into the
sample loop. When using the needle and sample inlet
tube supplied by Biotage, it must be set to 2 mL.
» Flush Volume for Needle and Sample Inlet Tube (mL): The
amount of system solvent that is used to purge the needle and
sample inlet tube (to remove air) before moving to the next
source tube in the same job. Recommended volume is 2 mL.
» Sample Aspiration Rate (mL/min): The flow rate when
aspirating sample solution into the sample loop.
Note: The default rate (10 mL/min) should work for most
solvents. However, we suggest a lower aspiration rate for
solvents with high viscosity or low boiling points.
» Sample Dispense Rate (mL/min): The flow rate
when dispensing sample solution from the
sample loop into the evaporation vial.
Note: This setting overrides the Dispense Rate setting for
the used vial; see page 4.
» Use Carousel: Defines whether the carousel can be
used () or not () from V- Touch Control Centre.
Note: The carousel also has to be enabled in the System
Setup view on the touch screen; see page 4.
» Instrument IP Address: The IP address of the
V- Touch system that the external computer is
connected to. The address is found in the System
Setup view on the touch screen; see page 4.
» Automatic Defrost After Sample Volume (mL): If enabled, the
system will automatically defrost the condenser when the
specified amount of sample solution has been evaporated
using the liquid handler. Note that an automatic defrost is
done after a job is completed and not in the middle of a job.
» Sample Volume Since Last Automatic Defrost (mL):
The amount of sample solution that has been evaporated,
using the liquid handler, since the last automatic
defrost operation. If performing a manual defrost, the
counter can be reset by pressing Reset Counter.
Note: For information on system settings that are entered
through the touch screen, see page 4.
Clean the Needle and Vial Tubing
The needle and vial tubing are cleaned automatically (separately)
after the last sample aspiration in each job. The system solvent
that is used to clean the vial tubing (2.8 mL) is added to the vial
and evaporated.
It is also possible to clean the needle manually at any time when
the system is not processing by clicking . First the needle is
lowered into the drain station where the tubing is cleaned, then
into the wash station where the inside and outside of the needle
are cleaned. The amount of system solvent used in each step is
defined in the Settings dialog; see above.
Evaporate Using Biotage
®
V- Touch Control Centre

 Biotage®V-10 Touch User Manual | © Biotage 2019
Evaporate Using Biotage®V- Touch Control Centre
Set Up an Evaporation Job
Warning
» Keep your hands away from the vial loading arm and carousel
(if used) while the vial is being loaded or unloaded.
1. Start the Controller application.
2. If using a liquid handler:
a. Open the workspace that fits your setup; select Open
Workspace... from the File menu. To define a new
workspace; see page 17.
b. Load the racks and source tubes onto the liquid handler
bed. Ensure that they correspond to the workspace setup.
c. In the software, select the test tubes that contain the
sample solution for the job. A selected source tube is
highlighted in red; see Figure 23.
3. Set up the evaporation parameters. For more information,
see "Evaporation Parameters" below.
4. Click Add Rack Job or Add Prefilled Vial to add the job to the
queue. If using a carousel, the next free carousel position
(counting from “A”) is automatically assigned and displayed
in the Slot column in the job list. Put the vial into that
position. Otherwise, the vial has to be put into the lower
vial holder when prompted by the software. Ensure that the
correct upper and lower vial adapters are used, if applicable.
See Table 2 on page 1.
Note: If processing prefilled vials, ensure that the dispensed
volume does not exceed the maximum fill volume of the
selected vial; see Table 2 on page 1.
5. To set up another job, repeat step 2c through 4.
Evaporation Parameters
The following evaporation parameters are available in the
Controller application:
» User: The name of the user. The predefined user list has four
users (User A-D). To add a user and/or change a user name,
edit the users.txt file, located at C:\Program Files (x86)\
Biotage\Biotage V- Touch Control Centre, using a text
editor. Each user must be listed on its own line. Note that
Controller has to be restarted for the changes to take effect.
» Method: The evaporation method. For more information,
see "Evaporation Methods" on page 1.
Note: If using a liquid handler, only use methods that have
the parameter End Evaporation set to Auto or Timed; see
"Define a Method" on page 3.
» Vial Size: The selected vial size; see "Vials" on page 1.
» Sample Name: The sample name. Optional.
» Sample Volume (mL): The amount of sample solution that
shall be aspirated from each source tube in the job.
» Test Tube Rinse: Defines whether all the source
tubes except for the last one will be rinsed (Yes) or
not (No) after its content has been dispensed. The rinse
liquid will be added to the vial and evaporated.
» Volume (mL): The amount of system solvent to be
used to rinse in-between the source tubes.
» Last Tube Rinse: The number of times (none, once,
or twice) the last source tube will be rinsed after
its content has been dispensed. The rinse liquid
will be added to the vial and evaporated.
» Volume (mL): The amount of system solvent
to be used to rinse the last source tube.
» Redissolve After: Defines whether the
sample is to be automatically redissolved
after the evaporation (Yes) or not (No).
» Volume (mL): The amount of system solvent
to be used to redissolve the sample.*
» Speed (rpm): The rotational speed of the vial
(in rpm) during the redissolve operation.*
» Cycles: The number of spin cycles for
the redissolve operation.*
» Notes: A note about the job. Optional.
*Only enabled when the parameter Redissolve After is set to “Yes”.
Modify or Delete a Queued Evaporation Job
Modify a Queued Evaporation Job
An evaporation job can be modified before it has been started.
1. Select the job in the job list. If it is a rack job, the source
tubes are highlighted in purple.
2. To remove a source tube, click it. A removed vial shifts from
purple to blue.
3. To add a source tube, click it. An added tube is highlighted
in red.
4. Edit the desired evaporation parameters; see "Evaporation
Parameters" above.
5. When done, click Modify Selected Job to save the changes.
Note: If you want to cancel the changes, click an empty row in
the job list or deselect (click) the selected job.
Delete a Queued Evaporation Job
To delete a queued job, select it in the job list and click Delete
Selected Job. After an evaporation job has been deleted, its
carousel position (if used) becomes available for a new sample.
Note: It is not possible to delete a job that is being processed.


Evaporate Using Biotage®V- Touch Control Centre
Figure 23. The Controller application when setting up a rack job. Selected
source tubes are highlighted in red.
Figure 24.When selecting a queued rack job in the job list, the source
tubes are highlighted in purple and the assigned carousel position in red.
Save and Open a Job List
It is possible to save a job list and use it again.
Note: Any jobs that are currently in the job list will be deleted
when a job list is opened.
To save a job list:
1. Select Save Job List... from the File menu.
2. In the Save a JobList File dialog, select the folder to save the
job list in, enter the file name, and click Save.
To open a job list:
1. Select Open Job List... from the File menu.
2. In the Open dialog, select the desired job list and click Open.
Start the Queue
Warning
» The system can be controlled either through the touch screen
or through an external computer running V-10 Touch Control
Centre. When using V-10 Touch Control Centre, it is your
responsibility to ensure that you will not interfere with an
operation that is about to be started through the touch screen.
1. Ensure that the V- Touch Control Centre settings are
according to your requirements; see page 13.
2. Ensure that all the jobs in the job list have their source tubes
on the liquid handler bed and, if using the carousel, their
evaporation vials in the assigned positions in the carousel
sample holder.
When selecting a queued job in the job list, its source tubes
are highlighted in purple and the assigned carousel position
(if applicable) is highlighted in red (rack job) or green
(prefilled vial). See Figure 24.
3. If the carousel is not used, put the evaporation vial for the
first job into the lower vial holder. Ensure that the correct
upper and lower vial adapters are used, if applicable. See
Table 2 on page 1.
4. Click to start the first job in the job list.
5. If the carousel is not used and there is more than one
evaporation job in the job list, the Job Complete dialog
opens when an evaporation job is completed. Replace the
vial and click Yes to start the next evaporation job.
Note: If you click No, the text “Stopped” appears in the
upper right corner of the software and the next evaporation
will not start until you click .
6. When the system has processed all the jobs in the queue,
empty the drain collection vessel.
Figure 25.The Controller application when processing jobs. Blue source
tubes on the workspace are assigned to queued or failed jobs and green to
completed jobs. The following icons are used to display the status of a job:
= in progress, = completed, and = failed.

 Biotage®V-10 Touch User Manual | © Biotage 2019
Evaporate Using Biotage®V- Touch Control Centre
Monitor an Evaporation Job
When an evaporation job is being processed, the text “Running”
appears in the upper right corner of the software and the icon is
displayed to the left of the job name in the job list. See Figure 25.
The current vial temperature, vacuum pressure, and applied
heater power can be monitored in the Evaporate view on the
touch screen; see Figure 26.
The task in progress is displayed in the top right corner of the
touch screen during the evaporation phase and in the lower left
corner of the Controller application when the system is loading
and unloading the vial and pumping and moving liquids.
When an evaporation job is completed, the text “Completed”
appears in the upper right corner of the Controller application,
the source tubes change from blue to green, and the icon is
displayed to the left of the job name in the job list.
Edit an Evaporation Job in Progress
During the evaporation run, it is possible to increase the target
temperature and/or decrease the target pressure in the Evaporate
view on the touch screen. To change these values, simply press
the Target Temperature field and/or the Target Pressure field.
Unload and Clear an Evaporation Job
Warning
» Keep your hands away from the vial loading arm and carousel
(if used) while the vial is being loaded or unloaded.
» Handle chemical and liquid waste according to the Safety Data
Sheets and to local/national guidelines on laboratory safety
procedures.
When a completed job is selected in the job list, the carousel
position (if applicable) is highlighted in red (rack job) or green
(prefilled vial). The source tubes are green and labeled with the
same letter as the carousel position.
When a failed job is selected in the job list, the source tubes are
highlighted in purple and the carousel position in red (rack job)
or green (prefilled vial).
To clear carousel and/or rack positions, select one of the
following options form the Clear menu:
» All Completed Tubes: Clears all rack positions
that are assigned to completed jobs.
» All Completed Jobs: Clear all carousel and rack
positions that are assigned to completed jobs.
» All Failed Jobs. Clear all carousel and rack
positions that are assigned to failed jobs.
Pause an Evaporation Job
To pause a job, click . Note that task in progress will be
completed and the pressure will be equalized before the job is
paused and the text “Paused” appears in the upper right corner
of the software. If pausing when the system is evaporating, the
evaporation will be completed and the vial will be unloaded
before the job is paused. Resume the processing by clicking .
Stop an Evaporation Job
To abort an evaporation job, click . Any sample solution that
has been aspirated but not dispensed into the evaporation vial
will be returned to the source tube. Note that the evaporation vial
will not be unloaded until the pump operation in progress (if any)
has been completed and the pressure has been equalized.
When an evaporation job has been stopped, the text “Cancelled”
appears in the upper right corner of the software and the icon
is displayed to the left of the job name in the job list. To re-start
the job, select the job, click Re-Submit Job, and then click .
To abort a pump operation, e.g. in case of leakage, switch off (O)
the system using the mains switch on the left side of the system.
Log Files
Job information is logged in a dated log file at C:\Program Files
(x86)\Biotage\Biotage V- Touch Control Centre\logs. A new
file is started each month (“WorkLog_YYYY_MM.xls”, where
“YYYY” represents the year and “M” is the number of the
month). The logs files are saved for a year.
It is also possible to export two more detailed log files
containing all the system events and errors that have occurred.
Export the logs by selecting Settings... from the File menu and
then pressing Export Log Files... in the Settings dialog.
Evaporate
Evaporation Mode Prelled Vials
Redissolve After
Redissolve Volume
Carousel Mode All Vials
NO YES
Total Volume
Remaining Volume ---
MaintenanceRedissolve Conguration Remote Control...
Target Temperature
Target Pressure
 °C
 mbar
Method
Current Temperature
Current Pressure
Current Power
Vial Size 20 mL
Aqueous
Stop
43 °C
8.1 mbar
53.0%
Figure 26.When a run is controlled by V-10 Touch Control Centre, the text
"Remote Control" is displayed in the top right corner of the touch screen when
the system is loading and unloading the vial and pumping and moving liquids.


Define Workspaces for a Liquid Handler
If you cannot use any of the predefined workspaces, you can
use the Editor application to create a workspace that allows
your racks to be loaded onto the liquid handler bed.
Note: Never run the Editor and Controller applications
simultaneously as only one of them will be able to communicate
with the system.
Create and Edit a Rack
1. Start the Editor application and select the Rack Editor tab.
The default rack is displayed; see Figure 27.
2. To edit or base a new rack on an existing one, select Open
Rack... from the File menu, select the desired rack, and then
click Open. The selected rack opens.
Note: Editing a rack does not alter the definition of that rack
in existing workspaces.
3. To create a new rack from scratch, select Clear Rack from the
Edit menu.
4. Enter the desired rack settings; see "Rack Parameters" below.
5. When done, select Save Rack... from the File menu. The
Save As dialog opens.
6. Select the folder to save the rack in, enter the file name, and
click Save. The predefined racks are located in the
Racks
and workspaces
folder (C:\Program Files (x86)\Biotage\
Biotage V- Touch Control Centre\Racks and workspaces).
Rack Parameters
The following rack parameters are available:
» Width: The maximum width of the rack in millimeters. Used
for defining the required footprint on the liquid handler bed.
» Length: The maximum length of the rack in millimeters. Used
for defining the required footprint on the liquid handler bed.
» RackOffset: The Xand Ycoordinates of the
top left corner of the rack in reference to the
SiteOffset position, in millimeters.
» SiteOffset: The Xand Ycoordinates of the centre of
the first test tube in millimeters. It is recommended
to be set to “0, 0”. It positions the test tube
array within the defined rack footprint.
» FlushDepth: The distance between the top of the test
tube and the flush position, in millimeters. Normally
the flush position will be a few millimeters below the
rim of the test tube, so that most of the test tube's inner
surface is flushed with system solvent when the Test Tube
Rinse and/or Last Tube Rinse parameters are enabled
(see "Set Up an Evaporation Job" on page 14).
» Pitch: The distance (in millimeters) between adjacent
sites within the rack, along the X- and Y-axes,
center-to-center of the test tube positions.
» Diameter: The diameter of the test tube (in millimeters)
sets the visual representation of the rack. It also affects
the test tube capacity (see the last bullet in the list).
» Count: The number of test tubes along the
X- and Y-axes. The total number of test tubes
is the product of these two numbers.
» Depth: The distance (in millimeters) from the
top to the bottom of the test tube. This affects
the test tube capacity (see the last bullet).
» TestTubeCapacity: The test tube capacity (in milliliters)
is calculated from the diameter and depth settings.
Figure 27.The rack editor with the default rack.
Create and Edit a Workspace
1. Ensure that the liquid handler has the Z-arm positioned at
175 mm and the needle is flush with the bottom of the needle
guide and not bent; see Figure 17 and Figure 18 on page 9.
2. Start the Editor application and select the Workspace Editor
tab. The default workspace (without racks) is displayed.
3. To edit or base a new workspace on an existing one:
a. Select Open Workspace... from the File menu. The Open
dialog opens.
b. Select the desired workspace and click Open. The
selected workspace with its racks opens.
c. To remove a rack, select the rack and click Remove.
d. Select each one of the racks that you want to keep and
ensure that they have the correct Rack Location settings;
see "Rack Location Settings" on page 18.
Define Workspaces for a Liquid Handler

 Biotage®V-10 Touch User Manual | © Biotage 2019
Define Workspaces for a Liquid Handler
Figure 28.The workspace editor showing the liquid handler settings (top)
and the rack location settings (bottom).
Verify the Setup of a Workspace
Verify Liquid Handler Settings
Verify the home, drain, and wash positions of the liquid handler
by selecting the following commands from the Go To list and
clicking Run.
» Home: Moves the needle to the home
position of the liquid handler.
» Drain: Lowers the needle into the drain station. This
position is decided by the Drain and DrainDepth
parameters in the workspace setup; see above.
» Wash: Lowers the needle into the wash station. This
position is decided by the Wash and WashDepth
parameters in the workspace setup; see above.
Note: To reduce the risk of bending the needle, loosen the
needle using the needle holder screw before using the Wash
command.
Verify Rack and Rack Location Settings
Note: Editing a rack does not alter the definition of that rack in
an existing workspace.
To reduce the risk of bending the needle and/or breaking a test
tube, we recommend the following procedure:
1. Ensure that the liquid handler has the Z-arm positioned at
175 mm and that the needle is flush with the bottom of the
needle guide and not bent; see Figure 17 and Figure 18 on
page 9.
2. Verify the Rack Location settings (see page 18) for each
rack:
a. Remove the first test tube (top left) in the rack and select
its position in the software.
4. To insert a rack:
a. Click Insert.... The Open dialog opens.
b. Select a rack and click Open. The selected rack is then
floating in the workspace, moving with the cursor.
c. Optionally, modify the setting in the Snap list.
This setting controls the accuracy of the cursor.
Recommended value is 1 mm (default).
d. Click to position the rack. The rack is dropped onto the
liquid handler bed.
e. Ensure that the rack has the correct Rack Location
settings; see "Rack Location Settings" below.
5. To move a rack, either select it and change the Xand/or Y
coordinates under Rack Location or right-click the rack and
select Move Rack in the appearing menu.
6. When the workspace setup is completed, select Save
Workspace.... from the File menu. The Save As dialog opens.
7. Select the folder to save the workspace in, enter the file name,
and click Save. The predefined workspaces are located in the
Racks and workspaces folder (C:\Program Files (x86) \Biotage\
Biotage
V- Touch Control Centre\Racks and workspaces).
8. Verify the workspace setup before using it; see below.
Workspace Parameters
Liquid Handler Settings
Note: If the liquid handler is set up correctly (see Figure 17 and
Figure 18 on page 9), the default settings for the Liquid
Handler parameters should be used.
» Drain: The Xand Ycoordinates of the drain station, which is
used for dispensing waste liquid, in millimeters. Default: 3,2.
» DrainDepth: The distance between the bottom
of the needle guide (needle home) and the
drain position, in millimeters. Default: 40.
» Wash: The Xand Ycoordinates of the wash station,
which is used for cleaning the inside and outside
of the needle, in millimeters. Default: 3,21.
» WashDepth: The distance between the bottom
of the needle guide (needle home) and the wash
position, in millimeters. Default: 110.
» Workspace: The area where the needle can reach along the
X-, Y- and Z-axes in millimeters; the green area in Figure 28.
The measurements are based on the used liquid handler
and cannot be changed. Gilson GX-271: 389 x 294 x 175.
Rack Location Settings
» X, Y: The X and Y coordinates of the centre of the first test
tube in the rack in reference to the top left corner of the
workspace area (the home position), in millimeters.
» Z: The distance between the rim of the test tube and the
bottom of the needle guide (needle home), in millimeters.