Endress+Hauser Analytik Jena CyBio SELMA User manual

CyBio®SELMA
User Manual

CyBio®SELMA 2016 III

Registered trademarks:
CyBio®. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Analytik Jena AG, Germany
TitletoallothertrademarksorbrandswhicharereferencedinthisUserManualbelongs
totheirlegalowners.
Copyright ©Copyright2016AnalytikJenaAG
Allrightsreserved.Nopartofthisdocumentationmay
beduplicated,photocopied,savedtoastoragesystem
ortransferredtoelectronicmediawithoutpriorwritten
permissionofthepublisher.
Publisher
AnalytikJenaAG
Konrad-Zuse-Strasse1
07745Jena
Germany
Tel:
 +4936417770
Fax:+493641779279
ServiceSupport
Tel:
 +493641779449
E-Mail:[email protected]
Ordering number OL7001-26-21XBLE05
Document type TranslationoftheoriginalUserManual
Serial number Refertonameplateattheproduct

CyBio®SELMA 2016 V

CE-Erklärung_Maschinenrichtlinie Revision 02 (2016)
Gültig ab: 20.04.2016
EG Konformitätserklärung
EC Declaration of Conformity
Name und Anschrift des Herstellers:
Name and address of the manufacturer:
Analytik Jena AG
Konrad-Zuse-Straße 1
D-07745 Jena
Hiermit erklären wir, dass das nachstehend beschriebene Produkt
Herewith we declare, that the product described below
CyBio®-/CyBi®-SELMA
OL7001-26-2XX
allen einschlägigen Bestimmungen der Maschinenrichtlinie 2006/42/EG entspricht. Das
Produkt entspricht zusätzlich den Bestimmungen der europäischen Richtlinie 2014/30/EU über
elektromagnetische Verträglichkeit. Die Sicherheitsziele der Niederspannungsrichtlinie werden
berücksichtigt.
is complying with all corresponding requirements of the Machinery Directive 2006/42/EC.
In addition the product is in conformity with the EC Directive relating to electromagnetic
compatibility 2014/30/EU. The safety objectives of the Low-Voltage Directive are taken into
account.
Angewandte harmonisierte Normen:
Harmonized Standards used:
EN ISO 12100
Sicherheit von Maschinen - Allgemeine
Gestaltungsleitsätze - Risikobeurteilung und
Risikominderung
Safety of Machinery - General principles for
design - Risk assessment and risk reduction
EN 61010-1 Sicherheitsbestimmungen für elektrische
Mess-, Steuer-, Regel- und Laborgeräte
Safety requirements for electrical equipment
for measurement, control and laboratory use
EN 61000-6-2 EMV - Fachgrundnorm: Störfestigkeit
Industriebereiche
EMC - Generic standards: Immunity for
industrial environments
EN 61000-6-4 EMV - Fachgrundnorm: Störaussendung
Industriebereiche
EMC - Generic standards: Emission for
indutrial environments
Bevollmächtigter für die Zusammenstellung der relevanten technischen Unterlagen:
The person authorized to compile the relevant technical documentation:
Analytik Jena AG, Konrad-Zuse-Straße 1, D-07745 Jena
Diese Erklärung bezieht sich nur auf das Produkt in dem Zustand, in dem es in Verkehr gebracht wurde; vom Endnutzer
nachträglich angebrachte Teile und/oder nachträglich vorgenommene Eingriffe bleiben unberücksichtigt. Die Erklärung verliert ihre
Gültigkeit, wenn das Produkt ohne Zustimmung umgebaut oder verändert wird.
This declaration relates exclusively to the product in the state in which it was placed on the market, and excludes components which
are added and/or operations carried out subsequently by the final user. The declaration is no more valid, if the product is modified
without agreement.
11

VI 2016 CyBio®SELMA


CyBio®SELMA 2016 VII

Table of contents
1 General Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 NotesRegardingThisUserManual................................1
1.2 TargetGroup................................................1
1.3 Conventions.................................................2
1.3.1 WarningNotes/WarningSigns .................................2
1.3.2 ImportantInformation .......................................2
1.4 ConformingUse..............................................3
1.5 Standards&Guidelines.........................................4
1.6 Warranty&Liability............................................4
1.7 ScopeofSupply..............................................4
2 Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 GeneralData.................................................5
2.2 OperatingData...............................................7
3 Safety Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 GeneralNotes................................................9
3.2 SafetyLabellingProvidedattheSystem.............................9
3.3 DangerZones&ProtectiveDevices...............................11
3.4 RequirementsOnOperatingPersonnel............................11
3.5 SpecificProductSafetyNotes...................................12
3.5.1 Operation ...............................................12
3.5.2 Transportation ............................................12
3.5.3 Maintenance&Care .......................................12
3.6 AdditionalSafetyNotes........................................13
3.6.1 HandlingofDangerousSubstances ............................13
3.6.2 ChemicalResistance........................................13
3.7 RulesofConductinaCaseofEmergency..........................14
4 Technical Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 GeneralSystemOverview......................................15
4.1.1 OperationControls ........................................16
4.1.2 Tray ....................................................18
4.1.3 ExternalPowerPack........................................19
4.1.4 Terminals&Nameplate .....................................19
4.1.5 CoverCaps&Connectors ...................................20

VIII 2016 CyBio®SELMA
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4.1.6 ExternalDisplay...........................................20
4.2 OperatingModes............................................21
4.2.1 OperationinSittingPosition..................................21
4.2.2 OperationinStandingPosition................................21
4.3 ProductVersions.............................................21
4.4 Function...................................................22
5 Transportation & Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.1 Transportation..............................................23
5.2 Storage....................................................25
6 Initial Start-Up & Routine Start-Up Procedure . . . . . . . . . . . . . . . . . . 27
6.1 SiteRequirements............................................27
6.1.1 InstallationRequirements ....................................27
6.1.2 EnergySupplies...........................................27
6.2 StartingUp.................................................28
6.3 FunctionTesting.............................................32
6.3.1 PrecisionTest .............................................32
6.3.2 AccuracyTest.............................................35
6.3.3 LeakTest ................................................36
7 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.1 OperationControls...........................................37
7.2 EnteringParametersattheTouchScreen..........................38
7.3 TouchScreen–MainMenu.....................................39
7.3.1 Priming .................................................40
7.3.2 Pipetting ................................................43
7.3.3 Dispensing ...............................................46
7.3.4 Diluting.................................................51
7.3.5 ReversePipetting ..........................................55
7.3.6 SerialDilution ............................................59
7.4 ExtendedFunctions...........................................63
7.4.1 TipChange ..............................................63
7.4.2 SystemSettings...........................................64
7.4.3 Saving&LoadingofParameterSets ............................67
7.5 IdleState...................................................69
7.6 SpecificSystemFeatures(PipettingHead)..........................70
7.6.1 Aspirationwithadditionalstroke..............................70
7.6.2 Aspirationwithoutadditionalstroke ...........................71

CyBio®SELMA 2016 IX

7.7 PrecisionPerformance(PipettingHead)............................72
8 Fault Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
8.1 FaultyValueEntries...........................................75
8.2 TipMagazine...............................................75
8.3 InterruptionofPipettingRoutinewithSTOPButton...................76
8.4 ErrorHandling...............................................77
8.4.1 SystemMalfunctionorFailureSituations ........................77
8.4.2 InitializationorProgramSequencingFaults ......................78
8.5 PowerFailure...............................................79
9 Maintenance & Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
9.1 SafetyNotes................................................81
9.2 MaintenanceWork...........................................82
9.2.1 Overview ................................................82
9.2.2 CyBio®TipTray ...........................................83
9.2.3 SealingPlate .............................................83
10 Shutting Down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11 Accessories & Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
11.1 Accessories.................................................87
12 Waste Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.1 Consumables...............................................89
12.2 Reagents...................................................89
12.3 System&Components........................................90

X2016CyBio
®SELMA


CyBio®SELMA 2016 1
GeneralInformation
1 General Information
1.1 Notes Regarding This User Manual
ThisUserManualinformsaboutthesetupandfunctionofthe
CyBio®SELMA.
Itprovidesqualifiedoperatingpersonnelwiththeknowledgethatisrequiredtohandle
thesysteminasafemanner.Italsocontainsadviceregardingsystemmaintenanceand
careandexplainspotentialcausesandactionforremovalofmalfunctions.
YoushouldalwayskeepthisUserManualwithineasyreachofthesystem.ThisManual
mustbereadilyavailabletooperatingandmaintenancepersonnelatanytime!
ThisUserManualisanintegralpartofthesystemandmustbehandedovertothenext
systemowneroruserasmaybeappropriateifthereisachangeonownership.
1.2 Target Group
ThisUserManualaddresses:
Qualifiedexpertpersonnelhavingcompletedaspecialtrainingonsystemopera-
tionandmaintenance(
refer to “Requirements On Operating Personnel” on page 11).
Personnelresponsiblefortheplanningofproceduralsequences,maintenance
andcleaningwork,safetyprecautions,etc.
NOTE
Alldataandinformationprovidedhereinreflectthelateststateofknowledgeavailableat
themomentofpublishing.AnalytikJenaAGreservestherighttomakechangesif
deemednecessaryandappropriateintheinterestoftechnicalchange.

22016CyBio
®SELMA
GeneralInformation
1.3 Conventions
1.3.1 Warning Notes/Warning Signs
1.3.2 Important Information
WARNING
Designatesapotentiallydangeroussituationthatmayresultindeathorsevereinjury
unlessprevented.
CAUTION
Dangeroussituation!
Potentialconsequences:slightorminorinjuries.
NOTICE
Designatesapotentialdangerorasituationthatmayresultinmaterialdamage.
NOTE
Usefuloperatoradvice,involvesnohazardoussituation.
NOTE
Notesregardingenvironmentalprotection

CyBio®SELMA 2016 3
GeneralInformation
1.4 Conforming Use
Theowner/operatorwillberesponsibleforconformingsystemuse.
TheCyBio®SELMApipettingrobothasbeendevelopedformanualandsemi-automatic
processingofmicroplatesinchemicalandbiologicallaboratories.Inmedicalordiagnos-
ticenvironments,itsrangeofcapabilitiesislimitedtoR&D.
Basicsystemfunctionsincludetheaspirationofliquidfrom,andthedispensinginto,
reservoirs,microplates,includingindividualcolumnsthereof.
Thesystemmayonlybeoperatedbytrainedandqualifiedpersonnel
Refer to “Requirements On Operating Personnel” on page 11.
Thesystemmaybeoperatedinnowayandundernoconditionsotherthan
describedinthisUserManual.Inthefirstplace,thisappliestoelectricpowersup-
plyvalues,operatingconditionsandexplanatorynotesregardingsystemmainte-
nance,service,transportationanddisposal.
SafetynotesinthisUserManualmustbefollowedasacompulsoryrule.
Your are not allowed to:
operatethesysteminamedicallaboratory.
useexplosivesubstancesinthesystem.
operatethissysteminanexplosiveenvironment.
smokeorigniteanopenfireattheinstallationsite.
Asregardsthesafehandlingofdangeroussubstances(radioactive,infectious,toxic,
etching,combustibleandotherhazardoussubstances),theowner/operatorwillbere-
sponsibleinaccordancewiththeprovisionsunderstatutorylawandguidelinepolicies.
Thesameappliesintermsofcompliancewithenvironmentalprotectionrules(e. g.for
disposalofreagentsandconsumables).
NOTE
Allpreventivemeasuresarecoordinatedwiththeconforminguseoftheequipment.
Non-conformingusemaycauselossofprotection.

42016CyBio
®SELMA
GeneralInformation
1.5 Standards & Guidelines
TheCyBio®SELMAhasbeenbuilttomeetcurrentlyvalidrulesoftechnologyandgen-
erallyestablishedrequirementsonsafetyengineering.
Itsengineeringdesignisinlinewithfundamentalrequirementsonthesafetyandhealth
ofpersonsasapplicableunderlaws,standardsandguidelines,whichfactisconfirmed
byCElabelingandadeclarationofconformity(refer to page V).
AllstatementsonsafetyarederivedfromcurrentlyvalidregulationsoftheEuropean
Union.Forothercountries,relevantnationallawsandregulationsmustbeobserved.
1.6 Warranty & Liability
Theperiodofwarrantyandscopeofliabilitieswillbeasstipulatedunderbindinglaw
andprovidedforintheGeneralTermsofBusiness.
1.7 Scope of Supply
Systemdeliveryincludes:
CyBio®SELMA
Movabletray
Powerpack
Powercabletospecificnationalstandard
BoxwithCyBio®TipTray
UserManualwithECconformitydeclaration,quickguide
Optionalaccessories

CyBio®SELMA 2016 5
TechnicalSpecifications
2 Technical Specifications
2.1 General Data
Designation/Type CyBio®SELMA
Channels 96,384
Pipettinghead capableofmotorizedmotioninZ-direction
Memorycapacity10parametersetsperpipettingmode
96-channel pipetting heads
96-channelhead(25 μl)
Volumerange10.5 μl–25 μl selectableinstepsof0.01 μl
Precision(CV)1–>2μl–5μl2%
–>5μl–25μl1%
Tiptypes –10μlShallowWell(SW)
–25μlShallowWell(SW)
–60μlDeepWell(DW)
1)validfor10 μland25 μltips
96-channelhead(60 μl)
Volumerange 1 μl–60 μl selectableinstepsof0.01 μl
Precision(CV) –>3μl–5μl2%
–>5μl–60 μl1%
Tiptypes –10μlShallowWell(SW)
–25μlShallowWell(SW)
–60μlDeepWell(DW)
96-channelhead(250 μl)
Volumerange 5 μl–250 μl selectableinstepsof0.1 μl
Precision(CV) –>10μl–25 μl2%
–>25μl–250 μl1%
Tiptypes –250μlShallowWell(SW)
–250μlDeepWell(DW)
96-channelhead(1000 μl)
Volumerange 10 μl–1000 μl selectableinstepsof0.1 μl
Precision(CV) –>25μl–100 μl2%
–>100μl–1000 μl1%
Tiptypes –1000μlDeepWell(DW)

62016CyBio
®SELMA
TechnicalSpecifications
384-channel pipetting heads
384-channelhead(25 μl)
Volumerange10.5 μl–25 μl selectableinstepsof0.01 μl
Precision(CV)1–>2μl–5μl2%
–>5μl–25μl1%
Tiptypes –10μlShallowWell(SW)
–25μlShallowWell(SW)
–60μlDeepWell(DW)
1)validfor10 μland25 μltips
384-channelhead(60 μl)
Volumerange 1 μl–60 μl selectableinstepsof0.01 μl
Precision(CV) –>3μl–5μl2%
–>5μl–60 μl1%
Tiptypes –10μlShallowWell(SW)
–25μlShallowWell(SW)
–60μlDeepWell(DW)
Microplate formats
96,384 ShallowWell;DeepWell
Plate positions
2
Dimensional & weight details
Phys.dimensions
(WxH1xD) 307x480x325 mm
Weight2about18 kg
Weightwithaccessories
andpacking3about23 kg
196-channelhead(1000 μl):520 mm
296-channelhead(1000 μl):20 kg
396-channelhead(1000 μl):25 kg

CyBio®SELMA 2016 7
TechnicalSpecifications
2.2 Operating Data
Operating data
Utilityclass Bench-topdevice,closedroomfacilitiesinclean
condition
Protectionclass III(SELV)
Internalprotectionstandard IP10(DINEN60529:2000-09)
Radiointerferencesuppression
(EMCDIR)
DINEN55011(11/2007),limitvalueclassA
Noiseimmunity(EMCDIR) DINEN61000-6-2(3/2006)
Emittedinterference(EMCDIR) DINEN61000-6-4(9/2007)
Electricalsafetyoflaboratory
equipment(LVDIR)
DINEN61010-1(08/2002)
Gen.safety(Mach.DIR) DINENISO12100,(03/2011)
Operatingvoltage 24 VDC;2.5 Amax
Powerconsumption(operation) 60 W
Powerconsumption(standby) 5W
Airbornesoundemission <70 dB(A)
Interface RS232Cserviceport,Sub-D9-pole
External power pack
Inputvoltage 100–240 VAC;(±10 %);1.7 Amax.;50/60 Hz
Outputvoltage 24 VDC;2.5 Amax
Operating & storage conditions
Operation:
– Permissibleambienttemperature
– Permissiblerelativeairhumidity
+15 °Cto+37 °C
85 %at+30 °C
Storage&transportation:
– Permissibleambienttemperature
– Permissiblerelativeairhumidity
-10 °Cto+50 °C
85 %at+30 °C
Conditionoffloorbase stable,horizontal,dry,freefromvibration

82016CyBio
®SELMA
TechnicalSpecifications

CyBio®SELMA 2016 9
SafetyNotes
3SafetyNotes
3.1 General Notes
InadditiontothesafetynotesinthisManualandlocalsafetypracticesasmaybeappli-
cabletosystemoperationfromcasetocase,generallyestablishedaccidentprevention,
industriallabourprotectionandenvironmentalprotectionrulesmustbeconsideredand
dulyfollowed.
3.2 Safety Labelling Provided at the System
Affixedwarningsignsandsafetysymbolsareintegralpartsofthesystemandmustbe
followed!
Thesystemisdeliveredwiththefollowingwarningnotes/signsandsafetysymbols:
NOTE
ForyourownsafetyandtoensurefailsafeandreliableoperationoftheCyBio®SELMA
system,youshouldcarefullyreadthischapterbeforeanykindofstart-upwork!
CAUTION
Thereisdangeroffaultyactionwithpersonalinjuryormaterialdamageifwarningnotes
arenotinplace.
Warningnotesandsafetysymbolsmustnotberemoved!Checkwarninglabelsand
safetysymbolsforintactnessandcompletenessbeforeyoubeginanykindofstart-up
action.Donotproceedtostart-upifyouhaveidentifiedamissingordamagedwarning
noteorsafetysymbol!Adamagedormissingwarningnoteorsafetysymbolmustbe
replacedorinstalledimmediately.
Warning symbol Meaning Comment
Warnsofadangerpoint Warnsofmechanicalhaz-
ardfromsystempartsper-
formingmotionandof
dangerouschemicaland
biologicalsubstances
Warnsofbiologicalhazard Warnsofhazardswhere
chemicalorbiologicalsub-
stancesarehandled
Warnsofhandinjury Warnsofcrushing/pinch-
ingbymechanicallymov-
ingparts

10 2016 CyBio®SELMA
SafetyNotes
Warning notes affixed to the system
Locationofwarningnotesatthesystem:
Fig. 1: Warning notes affixed to the system
1 "Biologicalhazard"–warnsofdangerousbiologicalorchemicalsubstances
2 "Caution"–warnsofmechanicalhazardfromsystempartsperformingmotion
3 "Crushinghazard"–warnsofpotentialcrushorpincheventswithinthezoneoftip
mounting
Nameplate on external
power pack
Comment
Neveropenthepowerpack!
Replacethepowerpackintheeventofadefect.
Operationatadifferentsupplyvoltagemayleadto
destructionofelectricalorelectroniccomponents!
Claimsforwarrantyorliabilitywillbenullandvoidin
suchcases!
1
2
3