Endress+Hauser Analytik Jena SpeedMill PLUS User manual

Operating Manual
SpeedMill PLUS
Homogenizer

Manufacturer Analytik Jena GmbH
Konrad-Zuse-Strasse 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Technical Service AnalytikJenaGmbH
Konrad-Zuse-Strasse 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
General Information http://www.analytik-jena.com
Documentation Number 10-3280-002-23
Edition C (11/2021)
Technical Documentation Analytik Jena GmbH
© Copyright 2021, Analytik Jena GmbH
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

SpeedMill PLUS Table of contents
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Table of contents
1 Basic information............................................................................................................................................................ 5
1.1 About this user manual .................................................................................................................................... 5
1.2 Intended use ..................................................................................................................................................... 5
2 Security............................................................................................................................................................................ 7
2.1 Safety labeling on the device ........................................................................................................................... 7
2.2 Requirements for the operating personnel..................................................................................................... 7
2.3 Safety instructions for transport and commissioning..................................................................................... 7
2.4 Safety instructions: during operation .............................................................................................................. 8
2.4.1 General.............................................................................................................................................................. 8
2.4.2 Safety instructions – protection against explosion and fire........................................................................... 8
2.4.3 Safety instructions – electrical equipment ...................................................................................................... 8
2.4.4 Handling of auxiliary and operating materials and samples ......................................................................... 9
2.4.5 Safety instructions – maintenance and repair ................................................................................................ 9
2.5 Behavior during emergencies .......................................................................................................................... 10
3 Function and design ....................................................................................................................................................... 11
3.1 Design, connections and control elements ..................................................................................................... 11
4 Installation and commissioning..................................................................................................................................... 16
4.1 Installation conditions...................................................................................................................................... 16
4.2 Commissioning ................................................................................................................................................. 17
5 Operation ........................................................................................................................................................................ 19
5.1 Preparing the samples...................................................................................................................................... 19
5.2 Inserting samples in the sample holder .......................................................................................................... 20
5.3 Removing samples............................................................................................................................................ 22
5.4 Homogenizing the samples ............................................................................................................................. 22
5.5 The three operating modes.............................................................................................................................. 22
5.5.1 Continuous mode.............................................................................................................................................. 22
5.5.2 Interval mode.................................................................................................................................................... 23
5.5.3 Quick mode ....................................................................................................................................................... 25
5.6 Operating mode presets................................................................................................................................... 25
5.6.1 Continuous mode settings................................................................................................................................ 25
5.6.2 Quick mode settings ......................................................................................................................................... 26
5.7 Managing protocols.......................................................................................................................................... 27
5.7.1 Saving protocols................................................................................................................................................ 27
5.7.2 Loading protocols ............................................................................................................................................. 27
5.7.3 Starting protocols ............................................................................................................................................. 28
5.7.4 Editing protocols............................................................................................................................................... 29
5.7.5 Deleting protocols............................................................................................................................................. 29
5.8 Preset protocols ................................................................................................................................................ 30
6 Maintenance and care.................................................................................................................................................... 31
6.1 Cleaning ............................................................................................................................................................ 31
6.2 Changing the fuse............................................................................................................................................. 31
6.3 Switching the operating voltage...................................................................................................................... 32

Table of contents SpeedMill PLUS
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7 Transport and storage .................................................................................................................................................... 33
7.1 Preparing devices for transport ....................................................................................................................... 33
7.2 Transport........................................................................................................................................................... 34
7.3 Storage .............................................................................................................................................................. 34
8 Disposal ........................................................................................................................................................................... 35
9 Technical data................................................................................................................................................................. 36

SpeedMill PLUS Basic information
5
1 Basic information
1.1 About this user manual
Content This user manual describes the following homogenizer:
¡SpeedMill PLUS
The device is intended to be operated by qualified specialist personnel observing the op-
erating manual.
The operating manual provides information about the design and operation of the de-
vice and provides operating personnel the necessary know-how for the safe handling of
the device and its components. The user manual also provides information on maintain-
ing and caring for the unit.
Conventions Instructions for actions occurring in chronological order are numbered and combined
into action units.
Warnings are indicated by a warning triangle and a signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the System menu).
¡Buttons are indicated by square brackets (e.g., [OK]).
¡Menu items are separated by vertical lines (e.g., System | Device).
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which can cause death or very serious (possi-
bly permanent) injury.
CAUTION
Indicates a potentially hazardous situation which can cause slight or minor injuries.
NOTICE
Provides information on potential material or environmental damage.
1.2 Intended use
The SpeedMill PLUS homogenizer can mechanically reduce source material intended for
subsequent isolation and purification of DNA, RNA or proteins. As the samples only heat
up to a minimal degree, continuous operation is possible.

Basic information SpeedMill PLUS
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The device can reduce both hard and soft source material. Even very resistant source
materials like bones, cartilage, chitinous exoskeleton of insects or ticks can be homoge-
nized completely and reproducibly in a short period of time.
The homogenizer may only be used for the methods described in these operating in-
structions. Any other use is considered improper use.
Hazardous substances such as strong acids or alkaline solutions must not be handled by
the device, as these can damage both the device and the reaction vessels. Combustible
liquids or substances that can form explosive compounds must also not be handled by
the device.
The homogenizer may only be loaded with plastic reaction vessels. Glass vessels may
break.

SpeedMill PLUS Security
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2 Security
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Observe all safety instructions listed in this user manual and all messages and infor-
mation displayed on the monitor by the control and analysis software.
2.1 Safety labeling on the device
Warning and mandatory action labels have been attached to the device and must al-
ways be observed.
Damaged or missing warning and mandatory action labels can cause incorrect ac-
tions leading to personal injury or material damage. The labels must not be removed.
Damaged warning and mandatory action labels must be replaced immediately!
The following warning and mandatory action labels have been attached to the de-
vice:
Mandatory action
labels/information
symbols
Meaning Comment
Disconnect the power
supply before opening
the device cover.
At the power switch/power inlet: Be-
fore opening the device cover, switch
off the device and disconnect the
power plug from the power socket.
For People's Republic of
China only
The device contains controlled sub-
stances. Analytik Jena GmbH warrants
that these substances will not be re-
leased from the device within the next
25years provided the device is em-
ployed as intended.
2.2 Requirements for the operating personnel
The device must only be operated by qualified specialist personnel instructed in the
use of the device. This instruction also includes conveying the contents of these oper-
ating instructions.
In addition to the safety instructions in this user manual, the general applicable
safety and accident prevention regulations of the respective country the device is op-
erated in must be observed and adhered to. The operator must determine the latest
version of these regulations.
The user manual must be accessible to the operating and service personnel.
2.3 Safety instructions for transport and commissioning
¡Insufficiently secured components pose a risk of injury. During transport, secure
the device components as specified in this operating manual.

Security SpeedMill PLUS
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¡Risk of damage to health due to improper decontamination! Perform a profes-
sional and documented decontamination of the device before returning it to An-
alytik Jena. The decontamination report is available from Service when register-
ing the return. Service must refuse acceptance of contaminated devices. The
sender may be liable for damage caused by inadequate decontamination of the
device.
2.4 Safety instructions: during operation
2.4.1 General
The operator must make sure that the device and its safety equipment is in sound
condition each time before starting up the device. This applies in particular after
each modification or extension of the device or its repair.
Observe the following:
¡The device may only be operated if all items of protective equipment (e.g. covers
in front of electronic components) are in place, properly installed and fully oper-
ational.
¡The sound condition of the protection and safety equipment must be checked
regularly. Any defects must be corrected as soon as they occur.
¡Protective and safety equipment must never be removed, modified or switched
off during operation.
¡Keep all combustible materials away from the device.
¡Ensure that no liquid enters the interior of the device, for example at cable con-
nections. There is a danger of electric shock.
¡The ventilation equipment on the device must be in good working condition.
Covered ventilation grilles or slots etc. may cause the device to break down or
may cause damage to it.
¡Modifications, conversions and extensions to the device are only permitted after
consultation with Analytik Jena. Unauthorized modifications can jeopardize the
device’s operational safety and may lead to limitations regarding the warranty
and access to customer service.
¡Hazardous substances such as strong acids or alkaline solutions and combustible
liquids or substances that can form explosive compounds must also not be han-
dled by the device. These can damage the device and cause injury to the operat-
ing personnel.
¡Only plastic reaction vessels may be used in the homogenizer. Glass vessels may
break and lead to injuries.
2.4.2 Safety instructions – protection against explosion and fire
The device may not be operated in an explosive environment.
Smoking or handling open flames are prohibited in the room in which the device is
operated!
2.4.3 Safety instructions – electrical equipment
Lethal voltages may occur in the device! Contact with live components may cause
death, serious injury or painful electrical shock.
¡The power plug must be connected to a proper power outlet to ensure that the
device meets protection class I (ground connector). The device may only be con-
nected to power sources whose nominal voltage is the same as that on the rating

SpeedMill PLUS Security
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plate of the equipment. Do not replace the removable power cable of the device
with a power cable that does not meet the specifications (with no protective
ground conductor). Extensions of the supply cable are not permitted!
¡Work on the electronics may only be carried out by the customer service of Ana-
lytik Jena and specially authorized technicians.
¡Before opening the device, the device must be switched off via the main switch
and the power plug must be disconnected from the power outlet!
¡Only use fuses of the specified type.
¡Ensure that the device is set to the correct operating voltage (→ "Switching the
operating voltage" 32).
2.4.4 Handling of auxiliary and operating materials and samples
The operator is responsible for the selection of substances used in the process as well
as for their safe handling. This is particularly important for radioactive, infectious,
poisonous, corrosive, combustible, explosive and otherwise dangerous substances.
When handling hazardous substances, the locally applicable safety instructions and
instructions in the safety data sheets from the manufacturers of the auxiliary and op-
erating materials must be complied with.
Observe the following:
¡The operator is responsible for carrying out suitable decontamination should the
device become contaminated externally or internally with dangerous substances.
¡Splashes, drops or larger liquid spillages should be removed using an absorbent
material such as cotton wool, laboratory wipes or cellulose.
¡For biological contamination, wipe the affected area with a suitable disinfectant,
such as an Incidin Plus solution. Then wipe the cleaned areas so that they are
dry.
¡The only suitable cleaning method for the housing is wipe disinfection. If the dis-
infectant has a spray nozzle, apply disinfectant to a suitable cloth before using it
on the device.
Particular care must be taken if infectious materials are analyzed with the device,
as the device cannot be decontaminated as a single unit.
¡Before using a cleaning or decontamination procedure other than that pre-
scribed by the manufacturer, the user is required to check with the manufacturer
that the intended procedure will not damage the device. Safety labels attached
to the device must not have methanol applied.
As a possible disinfectant, Analytik Jena recommends:
¡Decosept AF (for spray disinfection) from Dr. Schuhmacher GmbH
¡Meliseptol HBV disinfectant wipes from B. Braun
2.4.5 Safety instructions – maintenance and repair
The device is generally maintained by the customer service department of Analytik
Jena or specialist personnel trained and authorized by them.
Unauthorized maintenance can damage the device. For this reason, only the activi-
ties described in the user manual in the "Maintenance and care" chapter may be per-
formed by the operator.
¡All maintenance and repair work on the device must only be carried out when
the device is switched off (unless specified otherwise).
¡Before replacing device fuses or changing the operating voltage, disconnect the
power cord from the power supply.
¡Use only original spare parts, wear parts and consumables. These have been
tested and ensure safe operation.

Security SpeedMill PLUS
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2.5 Behavior during emergencies
¡If there is no immediate risk of injury, switch off the device and the connected
system components immediately in hazardous situations or in the event of an
accident and/or disconnect the power plugs from the power outlets.

SpeedMill PLUS Function and design
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3 Function and design
The SpeedMill PLUS homogenizer can mechanically reduce source material intended for
subsequent isolation and purification of DNA, RNA or proteins. As the samples only heat
up to a minimal degree, continuous operation is possible.
The device can reduce both hard and soft source material. Even very resistant source
materials like bones, cartilage, chitinous exoskeleton of insects or ticks can be homoge-
nized completely and reproducibly in a short period of time.
The handling of the device, as well the attachment and removal of the sample holder, is
very easy. Tools are not required.
The sample holder can be stored at temperatures as low as -80°C. This passively cools
the samples.
IST Innuscreen GmbH, close Analytik Jena cooperation partner, provides reaction vessels
with volumes of 0.5ml and 2.0ml for homogenization. The reaction vessels, provided
under the name innuSPEED Lysis Tubes, are filled with beads. There are various beads
for hard and for soft materials.
Beads are small balls that differ in material, hardness and ball size. The beads are accel-
erated during homogenization, thus grinding down the source material.
After the samples have been mechanically reduced in the homogenizer, nucleic acid ex-
traction follows. During this, lysis, DNA/RNA binding, washing and elution take place.
Devices for automatic nucleic acid extraction can be found in the Analytik Jena portfolio.
3.1 Design, connections and control elements
Front view
Figure1 Front view
1 Lid 2 Display
3 Keyboard 4 Touch sensor with LED status display

Function and design SpeedMill PLUS
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1
2
Figure2 Interior view (with sample holder)
1 Lid 2 Sample holder
The homogenizer is a standalone device. No PC with control and monitoring software is
required.
Sample holder
The sample holder has three components. The three components are inserted in se-
quence in the sample chamber. The sample holder can be removed completely to clean
the sample chamber.
The sample holder provides space for 20 reaction vessels.
The user loads the source material into the reaction vessels and then places the reaction
vessels in the sample holder. The closure seals the reaction vessel in the sample holder.

SpeedMill PLUS Function and design
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1 2 3
Figure3 Sample holder, disassembled
1 Base plate 2 Holder for reaction vessels
3 Closure and upper cover
Analytik Jena provides special tube fasteners as optional accessories for reaction vessels
with mandrels (such as innuSPEED Lysis TubeQ). The tube fasteners replace the stan-
dard sample holder. The tube fasteners ensure optimized energy input during oscilla-
tion.
Figure4 Tube fasteners with innuSPEED Lysis TubeQ (with mandrels)

Function and design SpeedMill PLUS
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Terminals and switches on the rear of the device
1
2
3
4
5
6
7
Figure5 Terminals and switches on the rear
1 Lid 2 Ventilation opening
3 Power connection 4 Mains fuse
5 Power switch 6 Touch sensor switch
7 COM interface (for service)
The switch on the rear of the device can be used to active/deactivate the touch sensor.
Type plate The type plate is attached to the rear of the device. The type plate contains the following
information:
¡Manufacturer
¡Trade name
¡Serial number
¡Electrical connection data
¡WEEE marking
¡CE marking

SpeedMill PLUS Function and design
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Control elements
The keyboard, the display and the touch sensor are located on the front side of the ho-
mogenizer.
Figure6 Control elements
1 Screen 2 Keyboard
3 Touch sensor
with LED status display
The keys have the following functions:
▴▾◂▸ Direction keys for moving the cursor, parameter setting
[enter] Confirm a set/selected parameter
Start the homogenization process
[esc] Return to the last menu
Interrupt/cancel an ongoing homogenization process
The homogenizer can be set to Sleep mode with the touch sensor. The LED displays the
operating state of the device.

Installation and commissioning SpeedMill PLUS
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4 Installation and commissioning
4.1 Installation conditions
Ambient conditions ¡This laboratory device is designed for inside use.
¡Do not use the device in wet and damp environments. Keep the device surface clean
and dry.
¡The installation site must be free of drafts, dust and caustic fumes.
¡Do not locate the device near sources of electromagnetic interference.
¡Avoid mechanical shocks and vibrations.
¡Avoid direct sunlight and radiation from heaters onto the device. If necessary, pro-
vide air conditioning.
¡Do not use the device in explosion-hazard environments.
¡Keep the ventilation slits free and do not obstruct them with other devices.
¡The device can interfere with the operation of magnetic storage devices and sensi-
tive electronics. Install the device at a distance to such devices.
Climate requirements for installation and storage locations:
Operating temperature +5to+40°C
Humidity Max. 80% at +30°C
Storage temperature -40to+70°C
Humidity during storage ≤80%
Power supply
WARNING
Danger due to electrical voltage
¡Only connect the device to a properly grounded socket which complies with the volt-
age indicated on the device’s rating plate.
¡Do not use an adapter in the feeder.
The device operates on single-phase alternating current.
The installation of the electrical equipment in the laboratory must comply with the DIN
VDE 0100 standard. At the connection point, an electrical current in accordance with the
standard IEC 38 must be available.
Spatial requirements Device with closed lid (W x H x D) 155x260x305mm
Device with open lid (W x H x D) 155x310x420mm
The sample holder has to be removed from the device from above.
On the rear of the device, a distance of 50cm must be maintained to adjacent devices to
ensure proper airflow between the device and the ambient surroundings. Do not posi-
tion the device on a soft surface.

SpeedMill PLUS Installation and commissioning
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4.2 Commissioning
DANGER
Risk of electric shock
The device is intended for use at 115/230V. Operating the device with incorrect voltage
can result in electric shocks and destruction of the device.
ðThe operating voltage can be set via the fuse holder. The operating voltage is indi-
cated in the viewing window of the fuse holder.
¡Check that the operating voltage settings on the fuse holder match the mains volt-
age.
¡Change the operating voltage as necessary.
NOTICE
Damage to the electronics due to condensation
Condensation and temperature differences may damage the device electronics during
commissioning.
¡Allow the device to acclimatize for at least one hour after positioning it in the oper-
ating room before commissioning.
Figure7 Start screen
Figure8 Main menu
}Remove the device from the packaging.
}Check if the set operating voltage matches the mains voltage in the view-
ing window of the fuse holder.
}Connect the power cable to the power connection on the rear of the de-
vice. Connect the cable to the power supply.
}Switch on the device via the power switch on the rear of the device.
}The start screen appears on the display. Press [enter] to go to the main
menu.
üThe device is ready for operation.

Installation and commissioning SpeedMill PLUS
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Switching the device off }When the homogenization process has finished, the device can be switched off via
the power switch.
}Only switch off the device after the cooling period (at least 30s) has elapsed. The
device may overheat otherwise.
üThe device is switched off.
Alternatively: You can put the device into Sleep mode via the touch sensor. The device is
not completely switched off in Sleep mode. It continues to consume power.
}Touch the touch sensor for approximately 3s until it lights up in red.
üThe device is in Sleep mode: The touch sensor is lit in red. The display goes out.
The fans switch off.
Deactivating the touch sensor A switch for activating and deactivating the touch sensor can be found on the rear of the
device.
}Set the switch on the rear of the device to "0".
üThe touch sensor LED goes out. The device can only be switched on and off via
the power switch.

SpeedMill PLUS Operation
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5 Operation
5.1 Preparing the samples
Observe the following instructions when preparing the samples and operating the ho-
mogenizer:
¡Hazardous substances such as strong acids or alkaline solutions must not be han-
dled by the device, as these can damage both the device and the reaction vessels.
Combustible liquids or substances that can form explosive compounds must also not
be handled by the device.
¡The homogenizer may only be loaded with plastic reaction vessels. Glass vessels
may break.
¡If the lid is opened during homogenization, the device immediately stops operation.
Open system
Various consumables can be used with the homogenizer:
¡The innuSPEED Kit or Lysis Tubes from IST Innuscreen GmbH, for example
¡Plastic reaction vessels from other manufacturers
The use of reaction vessels from IST Innuscreen GmbH (innuSPEED) for homogenization
of the source materials is also recommended for other applications than isolation of nu-
cleic acid.
Sample preparation with innuSPEED Kits or innuSPEED Lysis Tubes
(from IST Innuscreen GmbH)
Optimum sample sizes Material Optimum sample size (approx.)
Plant matter
¡Soft plant matter such as soft leaves,
fruit
5x5mm
¡Material of medium hardness such as
conifers
2.5x2.5mm
¡Very hard material such as conifers or
very thick and hard leaves
1to1.5 x1to1.5 mm
Tissue
¡Soft tissue such as lung, kidney, brain,
spleen or liver tissue
5x5mm
¡Very hard tissue such as rodent tails or
cartilage
1x1mm
}Prepare as buffers and solutions as described in the kit manual. Observe all informa-
tion on the preparation of various source materials.
}Load the Lysis Tubes with the source material.
}Follow the instructions in the kit manual and add Lysis Solution or water to the sam-
ples in the Lysis Tubes.
}Set the homogenization time indicates in the kit manual.

Operation SpeedMill PLUS
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Preparing samples with other reaction vessels
CAUTION
Risk of injury from broken glass
Glass vessel can break during homogenization.
¡Only use plastic reaction vessels.
Observe the following when selecting reaction vessels:
¡Use plastic reaction vessels with screw-on caps.
¡The reaction vessels can have the following volumes: 0.5ml, 1.5ml or 2.0ml.
¡The reaction vessels must be filled with beads. Beads are small balls that break
down the cell walls during homogenization.
¡Analytik Jena cannot ensure proper homogenization results when reaction vessels
from other manufacturers are used.
Wet or dry homogenization can be used for samples.
Observe the following homogenization times:
Material Recommended homogenization time
(approx.)
Plant matter
¡Soft plant matter such as soft leaves,
fruit
1min
¡Material of medium hardness such as
conifers
2min
¡Very hard material such as conifers or
very thick and hard leaves
3min
Tissue
¡Soft tissue such as lung, kidney, brain,
spleen or liver tissue
0.5to1min
¡Very hard tissue such as rodent tails or
cartilage, and
¡other tissue such as insects and ticks
2x2min
5.2 Inserting samples in the sample holder
Observe the following when inserting reaction vessels in the sample holder:
¡Load the sample holder with at least two reaction vessels before homogenization.
¡Do not start the device without the sample holder and samples!
¡Load the sample holder so that the sample are balanced.
}Place the base plate of the sample holder on the pin in the sample cham-
ber.