Macherey-Nagel NANOCOLOR Advance User manual

MACHEREY-NAGEL
Spectrophotometer
NANOCOLOR®Advance
www.mn-net.com
Water Analysis
Quick start guide

2Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Quick start guide
Important information:
This separate quick start guide is delivered together with the spectrophotometer
NANOCOLOR®Advance. The quick start guide is not intended to be a comprehensive
operating instruction. Further information, including detailed operating procedures, can be
found in the complete operating instruction of the device. Read them in addition to this
quick start guide. Be sure to read the safety instructions in this quick start guide before
operating the unit.
MACHEREY-NAGEL
The NANOCOLOR®Advance is a spectrophotometer for mobile and stationary eval-
uation of MACHEREY-NAGELtest kits. Due to its robust and waterproof design it is
suitable for use in the laboratory as well as for analysis directly at the sampling point.
Read this quick start guide carefully before taking a measurement and follow the instruc-
tions in this manual.
Safety instructions
BE SURE TO READ THE FOLLOWING SAFETY INSTRUCTIONS CAREFULLY BEFORE
USING THE DEVICE.
Failure to follow these instructions may result in serious injury to the operator, malfunction
or damage of the device.
Keep this manual in a safe place for future reference.
Follow the safety notes and instructions in the operating manual and observe the stickers
and notices on the device.
Do not work on internal parts of the unit. Non-compliance will invalidate any warranty claim.
Use of the hazard warnings
DANGER
Indicates an imminent or potentially hazardous situation which, if not avoided, will result
in death or serious injury.
WARNING
Indicates an imminent or potentially hazardous situation which, if not avoided, could
result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury.

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
NOTICE
Indicates a situation which, if not avoided, may result in malfunction or damage to the
device. Information that claries facts in the text and that requires special attention.
Explanation of symbols
This symbol indicates that there is a risk of electric shock.
This symbol indicates that the designated area can become hot and should not
be touched without suitable protective measures.
This symbol indicates that the chemicals used have a corrosive eect. Observe
the safety measures in the laboratory and wear the prescribed protective
equipment. Observe the instructions in the current safety data sheets (SDS)of
the products used.
This symbol indicates that there may be a danger by using ammable
substances.
Explanations to the text. Tips and tricks for better handling.
Technical data
Specification Description
Type: Spectrophotometer with reference-detector-technology
(RDT)
Light source: Halogen lamp
Optical system: Single beam photometer with grating monochromator
Wavelength range: 340 nm–800 nm
Wavelength accuracy: ± 2 nm
Wavelength resolution: 1 nm
Wavelength calibration: Automatic
Wavelength selection: Automatic, barcode, manually
Wavelength
reproducability:
± 0.1 nm
Scan speed: 1 complete scan < 3 min
Spectral bandwidth: < 4 nm
Photometric range: ± 3.0 Abs
Photometric accuracy: 0.003 Abs at 0.0–0.5 Abs; 1 % at 0,5–2,0 Abs

4Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Specification Description
Photometric linearity: < 0.5 % at 0.5–2.0 Abs;
≤ 1 % at > 2 Abs with neutral glass lters at 546 nm
Stray light: < 0.5 %
Measurement modes: More than 200 preprogrammed tests and special methods;
99 free programmable methods; Absorbance; Transmission;
Factor; Kinetics, 2 point calibration; scan;
nephelometric turbidity measurement
Turbidity measurement: Nephelometric turbidity measurement (16 mm and 24 mm)
1–1000 NTU
Cuvette slot: Test tubes 16 mm and 24 mm OD;
Rectangular cuvettes 10 mm, 20 mm, 40 mm and 50 mm
Data memory: 1000 measured data sets / spectra; GLP-conform
Display: Backlit display
Operation: Barcode technology; icon-based menu guidance; capacitive
touch screen
Languages: DE/EN/FR/ES/PT/PL /NL
External light: Insensitive; open cuvette slot
Interfaces: LAN (CAT6; only use shielded cable of max. 20 m length)
2 × USB (Host), 1 × USB (Function)
Operating conditions: 10–40 °C, up to 80 % rel. humidity (not condensing)
Protections class: IP67
Update: Via USB-Stick
Dimensions: 325 × 280 × 130 mm (L × W × H)

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Initial operation
Step 1: Power supply
CAUTION
Multiple hazards: Only qualied personnel should carry out instructions described in this
step.
WARNING
Risk of electric shock: Make sure that the power cord is not damaged. Check the
suitability of the power source for the equipment.
CAUTION
Risk of injury due to the danger of tipping over due to slipping. Place the device on a at
surface. Do not stack the device.
NOTICE
The unit is designed for indoor and outdoor use. Do not expose the device to direct
sunlight. Protect the interfaces from the eects of moisture and dampness when used
outdoors.
WARNING
Fire hazard: Take care not to overload the mains socket. There is a risk of
overload and re.
Make sure that the power cord is not damaged. Check that the power source used is
suitable for the equipment.
WARNING
Risk of injury: Familiarizeyourself with the device before working with it and read this
document carefully. Do not use the device unless you have received instruction in its
use.
NOTICE
Defects of the power supply and housing can lead to malfunction of the device. If the
device shows an apparent breakage of the housing or a damaged power supply, it must
be taken out of operation.

6Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Fig. 1: Remove the interface cover
The spectrophotometer is equipped with an
internal rechargeable battery, which is locked
for transport. To deactivate the locking, the
instrument must be connected to a power
source before it is switched on for the rst
time. Fully charge the battery afterwards.
Remove the interface cover on the left side of
the device to connect the power plug (Fig. 1).
A B C D
Fig. 2: Interfaces
Insert the hollow plug of the power supply
unit into the charging socket of the device.
(Fig. 2). Attach the adapter corresponding to
your power connection to the supplied power
supply unit. Connect it to a power source.
A Chargingsocket
B LAN connection
C USB A interfaces
D USB B interface
Step 2: Choose language
Deutsch
English
Nederlands
Português
francais
espanol
italiano
Fig. 3: Setting the language
Switch the device on using the on/o button
below the display.
After a few seconds, the self-test of the device
starts. The rst time the unit is switched on,
it will automatically request the language and
region settings (Fig. 3). Select the desired
language and then the desired region from
the list displayed.
The language can also be changed after
switching on the device via the menu
Settings > Instrument settings > Language.
Step 3: Change the cuvette adapter
WARNING
Danger from contact with chemicals: The cell adapters may be contaminated
after regular use. Use gloves when removing the adapters.
NOTICE
The cuvette slot cover ensures tightness according to IP67. A corresponding tightness is
therefore only ensured if the cell slot cover is placed correctly.

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Fig. 4: Remove cuvette slot cover
The device has a cuvette slot inwhich dierent
types of cuvettes can be inserted by using
adapters. The cuvette adapter for 16 mm test
tubes and rectangular cuvettes (adapter A) is
installed on delivery. The cuvette slot cover is
also attached. Before using the device, grab
the cuvette slot cover at the back and pull it
to the front for removal. (Fig. 4).
Fig. 5: Remove cuvette adapter
To change the adapter, rst remove the
pre-installed adapter. You can remove the
adapter by pressing lightly on the innersurace
of the adapter and pulling it out at the same
time. Then insert the desired adapter into the
cuvette slot until it clicks into place.
Adapter A: 16 mm test tubes,
10, 20, 40, 50 mm rectangular
cuvettes
Adapter B: 24 mm test tubes
Step 4: Run a test
WARNING
Possible dangers of contact with chemical and biological substances.
Working with samples, reagents and corresponding accessories is associated
with dangers.
Wear suitable protective equipment when working with the cuvettes. Observe the safety
data sheet (SDS)of the test kit used.

8Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
en
NANOCOLOR®COD 160 REF: 985026
Overview
Thetest iss uitable for thep hot ometric det erminat ionofth ech emi-
cal oxy gen demand(C OD) .Th ete sti sin accor dan cew ith DIN ISO
15705–H45andisequivalenttoEPAStandardMethod5220D.
This test issu itab lefo rwater,was tewa terandsludge.
• Measuringrange:15–160mg/LO2(method0261)
• Num be rof te sts :2 0
• Wavelengthforphotometricdetermination:436/450nm
• Shelfli fe:12mo nth s
• Reactiontime:120minutes
• Storagetemperature:15–25°C
• Storageconditions:protectedfromsunlight,upright.
Method
Theorganiccompoundsofasampleareoxidizedbyheatingthe
sampleinamixtureofsulphuricacidandpotassiumdichromate.
Thechemicaloxygendemand(COD)ofawatersampleisthecon-
centrationofoxygenthatisequivalenttotheamountofpotassium
dichromateconsumed. Thechangeinthepotassiumdichromate
concentration is determined via the change in absorbance after
digestion.
Interferences
Theforeignmaterialsshownheredonotinterferewiththetestup
tot hei ndicatedco nce ntr ati ons (in mg/L).Thec umu lat ive effe cto f
differentinterferingionshasnotbeentested.
Datainmg/L:
• Cl-:1500
Important:Forsamplesinwhichthechloridecontentishigh,shake
thetubetosuspendtheprecipitatepriortoaddingthesample.If
thechloridecontent is greater than 1500 mg/L, you must dilute
Qualitycontrol
Themeasurementofablankvalueandastandardisrecommended
beforeeverymeasuringseriesasqualitycontrolmeasure.
Qualityda ta:
Thefollowingdataweredeterminedduringproductionaccordingto
ISO8466-1andDIN38402-A51:
• Num ber ofLOTs:7 5
• Standarddeviationofthemethod:±1.06mg/LO2
• Coe ffi cie nto fva ria tiono fth epr oce ss: ±1. 28%
• Confidenceinterval:±2.44mg/LO2
Specifieddataforprocedure:
• Sensitivity(absorbanceof0.010Acorrespondsto):2.18mg/L
O2
• Acc uracy ofa mea surem ent val ue:±8 .40 mg/ LO2
LOT-speciccerticatesareavailableatwww.mn-net.com.
Procedure
1. Openroundcellandholdslightlytilted
2. Pipette2mLofsampleintotesttube
3. Sealtesttubeandshakevigorously(usesafetybottle,cuvette
willbecomehot!).
4. Heatfor2hat148°Corfor30minat160°C
5. Takethetubefromtheheatingblock
6. Shakeagainafter10min.whilestillwarm
7. Cool toroomtemperature
8. Cleanoutsideoftesttube
9. Measure
Notes
TestasampleofCOD-freewater(REF918993)togenerateablank
Fig. 6: Test kit instruction
Perform the test according to the instruc-
tions of the respective test kit (Fig. 6). Read
them carefully to obtain accurate and reliable
results. Prepare the sample according to the
instructions.
Note: Clean all cuvettes with a lint-free
cloth before insertion to remove any
impurities or moisture from the cuvette
and to prevent soiling of the cuvette
slot.
Step 5: Run a measurement
NOTICE
Contaminated cuvettes can contaminate the cuvette slot and lead to incorrect
measurement results.
Fig. 7: Measurement of tube tests
Test tubes: Insert the barcoded tube into the
cuvette slot. The barcode is recognized and
the barcode is read automatically (Fig. 7).
For test kits without zero measurement the
result is displayed automatically after a few
seconds.
When using a test kit with zero measurement
the device rst prompts you to measure
the zero solution. The zero measurement is
triggered by pressing . Then the sample
is inserted and the sample is measured by
pressing .
Alternatively, the desired method can be called up by selecting from the lists in the MN-Tests
menu or by entering the test number in the corresponding menu.
Note: The rst time a method is called up, it is automatically started in sub-method 1.
The sub-method (chemical form) can be changed by clicking on the entry
"sub-method" in the result screen.

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Step 6: Result screen
Fig. 8: Display of result
The end of the measurement is announced by
an acoustic signal. The measurement result is
shown on the display (Fig. 8). In addition to
the measurementresult, information on wave-
length, absorbance and interfering turbidity is
also given, depending on the settings.
If several results are available for one method,
they are displayed next to each other in the
form of tabs.
Note: If the result of the measurement is outside the measuring range of the used test
kit, the result is displayed with "< measuring range" or "> measuring range". Below
the result an estimated value is displayed in square brackets.
Step 7: Enter sample information
Fig. 9: Sample information
Below the measurement result are the icons
for entering the sample information. The
information comment, sample location and
dilution are set here ex works.
You can change this selection and enter
the sample information by clicking on
the -icon. Use the sliders to set whether
the corresponding sample information is
displayed (Fig. 9).
The entered information is saved together
with the measurement result.

10 Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Absorbance measurement
Fig. 10: Enter wavelengths
An absorbance measurement can be
performed within the wavelength range of
340–800 nm for up to 20 dierent wave-
lengths simultaneously. By selecting Basic
functions > Absorbance, the desired wave-
lengths can be entered via λ(Fig. 10).
Conrm the entry with clicking on "Enter". The
entered value is accepted in the list. Press
to start the measurement process.
Insert the cuvette with the zero solution and
press . Insert the sample cuvette and
press .
NTU-Check
Fig. 11: NTU-Check
The NANOCOLOR®Advance provides
a warning of interfering turbidity when
measuring a test in the 16 mm and 24 mm
test tube.
The NTU-Check is activated ex works.
Parallel to the measurement, the nephelo-
metric turbidity is determined by measuring
at 860 nm at a 90° angle. The NTU value
is displayed together with the result (Fig.
11). If the preset warning limit of 10 NTU is
exceeded, a warning is issued and the result
is displayed in red. The measured turbidity
value is stored together with the result.
Cleaning
NOTICE
For all cleaning work, the unit must be switched o and disconnected from the
power supply.
WARNING
Fire hazard. Do not use ammable detergents or organic solvents to clean the
unit.
NOTICE
Do not clean the device with acetone or similar products.
NOTICE
Make sure that there is no cuvette and no cuvette adapter in the cuvette slot.

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
1. Wipe the outside of the device with a damp cloth. Use water or a mild detergent. Then
dry the unit with a soft cloth.
2. If necessary, clean or dry the touch screen with a soft, lint-free cotton cloth.
3. If necessary, clean or dry the cuvette slot and adapters with a soft, lint-free cotton cloth.
For details on cleaning and maintenance of the device, refer to the complete operating
instruction of the device.
Quality control
Each laboratory or facility must establish its own QC policy.
To check the performance of the test kits and the device used, we recommend measuring
standard solutions of known concentration within the measuring range.
The photometric accuracy of the NANOCOLOR®Advance can be checked with
the NANOCONTROL NANOCHECK 2.0 color solutions (REF 925703) oered by
MACHEREY-NAGEL.The halogen lamp can be checked by the internal lamp check (see
detailed operating instructions of the device). The wavelength accuracy can be checked
with the integrated holmium oxide lter (see detailed operating instructions of the device).
Maintenance
NOTICE
Malfunction or damage of the device due to incorrect maintenance. Maintenance of the
device may only be carried out by qualied personnel.
WARNING
Incorrect measurements: A dirty cuvette slot can lead to contamination of the inserted
cuvettes and false results. Check the cleanliness of the cuvette slot and the adapters at
regular intervals and during maintenance. Clean the cuvette slot and the adapters if they
are dirty (see chapter “Cleaning”).
For further maintenance information, follow the instructions in the complete operating
instructions of the device or contact MACHEREY-NAGEL.

12 Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Accessories and spare parts
NOTICE
Only use accessories and spare parts approved by the manufacturer. If parts not
approved by the manufacturer are used, the operator is responsible for the conformity of
the device and the warranty will become void.
For information on accessories and spare parts, refer to the complete operating instruction
of the device or contact MACHEREY-NAGEL.
Troubleshooting
Depending on the operating status, dierent messages can be displayed. The source of the
error can be either an operating error or a malfunction of the device.
In case of recurring errors, contact MACHEREY-NAGEL.
Error Cause Solution
The device does not
start
The battery is empty or the
device is not connected to a
power source.
Connect the device to a
power source. Charge the
battery of the device.
Wavelength is outside
the permitted range
(340–800 nm)
The wavelength range of
the device extends from
340–800 nm.
Enter a wavelength within the
range of 340–800 nm.
Data cannot be
exported
The connected data carrier
is damaged.
Reconnect the data carrier
and repeat the data export.
Initialization error The device was not
successfully initialized.
Restart the device. Contact
the technical customer
service.
Absorbance > 3.5 E The measured absorbance
is higher than 3.5.
Dilute the sample and repeat
the measurement. Check
the use of a cuvette with a
shorter path length.

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Spectrophotometer NANOCOLOR®Advance 1.0, 08.2020
Shipping the device
NOTICE
If the battery is damaged, the device must not be shipped. Contact MACHEREY-NAGEL.
NOTICE
When the equipment is shipped, the outer carton must be marked to indicate lithium ion
batteries in equipment (UN 3481).
If you have any questions about shipping the unit, follow the instructions in the complete
manual of the unit or contact MACHEREY-NAGEL.
Disposal
NOTICE
Disposal via public disposal systems is not permitted. Contact your local
MACHEREY-NAGELrepresentative.
Disposal according to EU Directive2012/19/EU. In accordance with the EU Directive
2012/19/EU MACHEREY-NAGELtakes back the old device and disposes it free of
charge.
MACHEREY-NAGEL GmbH & Co. KG
If you still have questions or need technical assistance after reading the manual, contact:
MACHEREY NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8 · 52355 Dueren · Germany
Phone: [phone]
Fax: [phone]
e-mail: [e-mail]
www.mn-net.com
Spectrophotometer NANOCOLOR®Advance

www.mn-net.com
DE Tel.: [phone] [e-mail]
CH Tel.: [phone] [e-mail]
FR Tel.: [phone] [e-mail]
US Tel.: [phone] [e-mail]
MACHEREY-NAGELGmbH & Co. KG
Neumann-Neander-Str. 6–8
52355 Düren · Germany
A066989 / 0900.5