Macherey-Nagel NucleoSpin RNA Clean-up XS User manual

www.mn-net.com
www.mn-net.com
DE Tel.: [phone] [email protected]
CH Tel.: [phone] [email protected]
FR Tel.: [phone] [email protected]
US Tel.: [phone] [email protected]
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8
52355 Düren · Deutschland
www.mn-net.com
www.mn-net.com
DE Tel.: [phone] [email protected]
CH Tel.: [phone] [email protected]
FR Tel.: [phone] [email protected]
US Tel.: [phone] [email protected]
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8
52355 Düren · Deutschland
Bioanalysis
MACHEREY-NAGEL
User manual
March 2021 / Rev. 05
nNucleoSpin®RNA Clean-up XS
RNA clean-up

3MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
Table of contents
1 Components 4
1.1 Kit contents 4
1.2 Reagents, consumables, and equipment to be supplied by user 5
1.3 About this user manual 5
2 Product description 6
2.1 The basic principle 6
2.2 Kit specifications 6
2.3 Handling, preparation, and storage of starting materials 7
2.4 Elution procedures 8
2.5 Stability of isolated RNA 8
3 Storage conditions and preparation of working solutions 9
4 Safety instructions 10
5 Protocols 11
5.1 RNA clean up and concentration of RNA 11
5.2 DNA digestion in crude RNA extracts and subsequent clean up 13
6 Appendix 14
6.1 Troubleshooting 14
6.2 Ordering information 16
6.3 Literature 17
6.4 Product use restriction / warranty 17

MACHEREY-NAGEL – 03/2021, Rev. 054
RNA clean up XS
1 Components
1.1 Kit contents
NucleoSpin®RNA Clean-up XS
REF
10 preps
740903.10
50 preps
740903.50
250 preps
740903.250
Clean-up Buffer RCU (Concentrate)* 5 mL 5 mL 5 x 5 mL
Wash Buffer RA3 (Concentrate)* 6 mL 12 mL 50 mL
RNase-free H2O 13 mL 13 mL 13 mL
NucleoSpin®RNA Clean-up XS
Binding Columns (light blue rings –
plus Collection Tubes)
10 50 250
Collection Tubes (2 mL) 10 50 250
Collection Tubes (1.5 mL) 10 50 250
User manual 1 1 1
* For preparation of working solutions and storage conditions see section 3.

5MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
1.2 Reagents, consumables, and equipment to be supplied
by user
Reagents
• 96–100 % ethanol (to prepare Wash Buffer RA3 and prepare Clean-up Buffer RCU and
Wash Buffer RA3)
Consumables
• 1.5 mL microcentrifuge tubes
• Sterile RNase-free tips
Equipment
• Manual pipettors
• Vortex mixer
• Centrifuge for microcentrifuge tubes
• Personal protection equipment (e.g., lab coat, gloves, goggles)
1.3 About this user manual
It is strongly recommended reading the detailed protocol sections of this user manual if the
NucleoSpin®RNA Clean-up XS kit is used for the first time. Experienced users, however,
may refer to the Protocol at a glance instead. The Protocol at a glance is designed to be used
only as a supplemental tool for quick referencing while performing the purification procedure.
All technical literature is available on the internet at www.mn-net.com.
Please contact Technical Service regarding information about changes of the current user
manual compared to previous revisions.

MACHEREY-NAGEL – 03/2021, Rev. 056
RNA clean up XS
2 Product description
2.1 The basic principle
A major aspect of RNA clean up is preventing degradation of the RNA during the clean
up procedure. The NucleoSpin®RNA Clean-up XS method achieves this by mixing the
crude RNA extract with a binding buffer, containing chaotropic ions, and ethanol. This buffer
immediately inactivates RNases (which are present in virtually all biological materials) and
creates appropriate binding conditions to allow adsorption of RNA to the silica membrane.
Two washing steps with a single buffer remove any impurities. Pure RNA is finally eluted at
low ionic strength conditions with RNase-free water (supplied) in a volume as small as 5 µL.
The RNA clean up procedure using NucleoSpin®RNA Clean-up XS kit can be performed
at room temperature. The eluate should be treated with care because RNA is very sensitive
to trace contaminations of RNases, often present on general lab ware, fingerprints, and dust.
To ensure RNA stability, we recommend keeping the RNA solution frozen at -20 °C for short-
term or -70 °C for long-term storage.
2.2 Kit specifications
• The NucleoSpin®RNA Clean-up XS kit is recommended for the clean up and
concentration of prepurified RNA samples. Typical sample material covers nanogramm
to microgramm amounts of prepurified RNA (e.g., phenol-purified RNA) and RNA from
reaction mixtures (e.g., DNase treated samples).
• The innovative column design with a funnel shaped thrust ring and a small silica
membrane area allows sample volumes of up to 300 µL and elution of RNA in as
little as 5–30 µL. Thus, highly concentrated RNA is eluted and is ready for common
downstream applications (e.g., RT-PCR). RNA enrichment of 20 x up to 50 x can be
achieved (e.g., input: 300 µL sample containing crude RNA (10 ng/µL); output: 5 µL
eluate containing pure RNA (510 ng/µL); enrichment of factor 51 (MACHEREY-NAGEL
in-house data)).
• The RNA recovery rate is typically 85–95 %.
• High quality RNA (RNA Integrity Number (RIN) > 9 according to Agilent 2100
Bioanylzer assays) can be obtained from high quality RNA samples. The RIN of the
processed sample is typically equal (±0.3) to the RIN of the input sample. RNA quality
always depends on the sample quality, see section 6.3 for further aspects.
• The NucleoSpin®RNA Clean-up XS kit allows clean up and concentration of RNA
with an A260/A280 ratio generally exceeding 1.9 (measured in TE buffer pH 7.5). Due
to the high RNA purity, large amounts of eluates can be used as template in RT-PCR
without inhibition (e. g., 8 µL of 10 µL eluates as template in a 20 µL qRT-PCR setup
generating stronger signal compared to reactions with less template in a LightCycler™
PCR with the Sigma SYBR®Green Quantitative RT-PCR Kit).

7MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
Table 1: Kit specifications at a glance
Parameter NucleoSpin®RNA Clean-up XS
Technology Silica membrane technology
Format Mini spin columns – XS design
Sample material < 300 µL RNA solution containing < 90 µg RNA
Fragment size > 200 nt
Typical recovery 85–95 %
A260/A280 1.9–2.1
Elution volume 5–30 µL
Preparation time Approx. 20 min/6 preps
Binding capacity 110 µg
2.3 Handling, preparation, and storage of starting materials
RNA intended to be used as sample for the NucleoSpin®RNA Clean-up XS procedure
should be handled with the same care as any RNA sample. The stability of prepurified
RNA samples (e.g., RNA isolated with phenol based protocols) depends very much on the
performed procedure.
Wear gloves at all times during the preparation. Change gloves frequently.

MACHEREY-NAGEL – 03/2021, Rev. 058
RNA clean up XS
2.4 Elution procedures
A high RNA concentration in the elution fraction is desirable for all typical downstream
applications. In particular with regard to limited volumes of reaction mixes, high RNA
concentration can be a crucial criterion. Due to a high default elution volume, standard kits
often result in low concentrated RNA, if only small samples are processed.
Such RNA often even requires a subsequent concentration to be suitable for the desired
application.
In contrast to standard kits, NucleoSpin®RNA Clean-up XS allows an efficient elution in a
very small volume resulting in highly concentrated RNA.
Elution volumes in the range of 5–30 µL are recommended, the default volume is 10 µL.
2.5 Stability of isolated RNA
Eluted RNA should immediately be put and always kept on ice during work for optimal
stability! Contamination with almost omnipresent RNases (general lab ware, fingerprints,
dust) may be a risk for isolated RNA. For short-term storage freeze at -20 °C, for long-term
storage freeze at -70 °C.

9MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
3 Storage conditions and preparation of working
solutions
Attention: Buffers RCU contains chaotropic salt. Wear gloves and goggles!
CAUTION: Buffer RCU contains guanidinium thiocyanate which can form highly reactive
compounds when combined with bleach (sodium hypochlorite). DO NOT add bleach or acidic
solutions directly to the sample-preparation waste.
• All kit components should be stored at room temperature (18–25 °C) and are stable up
to one year. Storage at lower temperatures may cause precipitation of salts.
• Check that 96–100 % ethanol is available as additional solution in the lab.
Before starting any NucleoSpin®RNA Clean-up protocol, prepare the following:
• Clean-up Buffer RCU: Add the indicated volume of 96–100 % ethanol to the Clean-up
Buffer RCU Concentrate. See table below or bottle label for necessary volumes. Store
Buffer RCU at room temperature (18–25 °C) for up to one year.
• Wash Buffer RA3: Add the indicated volume of 96–100 % ethanol (see table below) to
Wash Buffer RA3 Concentrate. Mark the label of the bottle to indicate that ethanol was
added. Store Wash Buffer RA3 at room temperature (18–25 °C) for up to one year.
NucleoSpin®RNA Clean-up
REF
10 preps
740903.10
50 preps
740903.50
250 preps
740903.250
Clean-up Buffer
RCU (Concentrate)
5 mL
Add 15 mL ethanol
5 mL
Add 15 mL ethanol
5 x 5 mL
Add 15 mL ethanol to
each bottle
Wash Buffer RA3
(Concentrate)
6 mL
Add 24 mL ethanol
12 mL
Add 48 mL ethanol
50 mL
Add 200 mL ethanol

MACHEREY-NAGEL – 03/2021, Rev. 0510
RNA clean up XS
4 Safety instructions
The following components of the NucleoSpin®RNA Clean-up XS kits contain hazardous
contents.
Wear gloves and goggles and follow the safety instructions given in this section.
Only harmful features do not need to be labeled with H and P phrases up to 125 mL or 125 g.
Mindergefährliche Eigenschaften müssen bis 125 mL oder 125 g nicht mit H- und P-Sätzen gekennzeichnet
werden.
Component Hazard contents GHS
symbol
Hazard
phrases
Precaution
phrases
Inhalt Gefahrstoff GHS-Symbol H-Sätze P-Sätze
RCU guanidinium thiocyanate
45–60 %
Guanidinthiocyanat 45–60 %
CAS 593-84-0
WARNING
ACHTUNG
302, 412 264W, 273,
301+312, 330
Hazard phrases
H 302 Harmful if swallowed.
Gesundheitsschädlich bei Verschlucken.
H 412 Harmful to aquatic life with long lasting effects.
Schädlich für Wasserorganismen, mit langfristiger Wirkung.
Precaution phrases
P 264W Wash with water thoroughly after handling.
Nach Gebrauch mit Wasser gründlich waschen.
P 273 Avoid release to the environment.
Freisetzung in die Umwelt vermeiden.
P 301+312 IF SWALLOWED: Call a POISON CENTER / doctor / … / if you feel unwell.
BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM/Arzt / … anrufen.
P 330 Rinse mouth.
Mund ausspülen.
The symbol shown on labels refers to further safety information in this section.
Das auf Etiketten dargestellte Symbol weist auf weitere Sicherheitsinformationen dieses Kapitels hin.
For further information please see Material Safety Data Sheets (www.mn-net.com).
Weiterführende Informationen finden Sie in den Sicherheitsdatenblättern (www.mn-net.com).
* Hazard labeling not neccessary if quantity per bottle below 125 g or ml (certificate of exemption according
to 67 / 548/EEC Art. 25, 1999 / 45/EC Art. 12 and German GefStoffV § 20 (3) and TRGS 200 7.1).
For further information see Material Safety Data Sheet.

11MACHEREY-NAGEL – 03/2021, Rev. 05
NucleoSpin®RNA Clean-up XS
5 Protocols
5.1 RNA clean up and concentration of RNA
Before starting the preparation:
• Check if Buffer RCU and Buffer RA3 were prepared according to section 3.
1 Sample preparation
Provide up to 300 µL sample containing up to 90 µg
RNA – such as prepurified RNA (e.g., phenol purified) or
RNA from reaction mixtures (e.g., labelling reactions) –
in a microcentrifuge tube (not provided).
For appropriate sample amounts see section 2.2.
Note: Fill up RNA samples smaller than 100 µL with
RNase-free water to 100 µL. RNA samples from
100–200 µL should be filled up with RNase-free water
to 200 µL.
2 Adjust RNA binding conditions
Add one volume of Buffer RCU to the sample (e.g.,
100 µL RCU to 100 µL sample) and mix 2 x 5 s. If
necessary, spin down gently (approx. 1 s at 1,000 x g)
to clean the lid.
+ 1 vol. RCU
Mix
(2 x 5 s)
3 Bind RNA
Take one NucleoSpin®RNA XS Column (light blue ring)
placed in a Collection Tube for each preparation. Load
up to 300 µL sample mix to the column. Centrifuge for
30s at 11,000 x g.
For volumes exceeding 300 µL, load the sample mix in
two subsequent centrifugation steps onto the column.
Place the column in a new Collection Tube (2 mL).
Maximal loading capacity of NucleoSpin®RNA XS
Columns is 600 µL. However, for maximum performance
loading at most 300 µL onto the column for one
centrifugation step is recommended. For larger volumes,
load the sample mix in two (or more if necessary)
successive centrifugation steps. Repeat the procedure if
larger volumes are to be processed. For high demanding
applications, the recovery rate can further be increased
as follows: Centrifuge 30 s at 2,000 x g prior to
centrifugation for 30 s at 11,000 x g.
Load sample
mix
11,000 x g,
30 s

MACHEREY-NAGEL – 03/2021, Rev. 0512
NucleoSpin®RNA Clean-up XS
4 Wash and dry silica membrane
1st wash
Add 400 µL Buffer RA3 to the NucleoSpin® RNA XS
Column. Centrifuge for 30 s at 11,000 x g. Discard
flowthrough and place the column back into the
Collection Tube.
+ 400 µL RA3
11,000 x g,
30 s
+ 200 µL RA3
11,000 x g,
2 min
2nd wash
Add 200 µL Buffer RA3 to the NucleoSpin® RNA XS
Column. Centrifuge for 2 min at 11,000 x gto dry the
membrane. Place the column into a nuclease-free
Collection Tube (1.5 mL, supplied).
If for any reason, the liquid level in the Collection Tube
has reached the NucleoSpin® RNA XS Column after
centrifugation, discard flowthrough and centrifuge again.
5 Elute RNA
Elute the RNA in 10 µL RNase-free H2O, (supplied) and
centrifuge at 11,000 x g. for 30 s.
If higher RNA concentrations or higher elution volumes
are desired, elution volume may be varied in the range
of 5–30 µL.
For further details on alternative elution procedures see
section 2.4.
+ 10 μL
RNase-free
H2O
11,000 x g,
30 s

13MACHEREY-NAGEL – 03/2021, Rev. 05
NucleoSpin®RNA Clean-up XS
5.2 DNA digestion in crude RNA extracts and subsequent
clean up
Several commonly used RNA purification methods co-purify DNA to a considerable
extent (e.g., phenol based RNA purification). This often requires a subsequent removal of
contaminating DNA and clean up of the RNA from the reaction mixture.
DNA digestion in solution can efficiently destroy contaminating DNA. However, stringent
RNase control and subsequent repurification of the RNA (in order to remove buffer, salts,
DNase, and digested DNA) are usually required.
The MACHEREY-NAGEL rDNase Set (to be ordered separately, see ordering information),
contains high quality, recombinant RNase-free DNase (rDNase) and reaction buffer. It is
optimized for a highly efficient digestion in order to remove even traces of contaminating
DNA.
1 Digest DNA (reaction setup)
Prepare enzyme-buffer premix: Add 1 µL rDNase to 10 µL Reaction Buffer for
rDNase.
Add 1 / 10 volume of enzyme-buffer premix to the crude RNA extract (e.g., to 10 µL
RNA extract add 1 µL of the premix comprising buffer and enzyme).
Gently swirl the tube in order to mix the solutions. Spin down gently (approx. 1s at
1,000 x g) to collect every droplet of the solution at the bottom of the tube.
Note: Dissolve lyophilized rDNase (rDNase Set, see ordering information) in 540 µL
RNase-free H2O as described in the corresponding user manual.
2 Incubate sample
Incubate for 10 min at 37 °C.
3 Repurify RNA
Repurify RNA with the NucleoSpin®RNA Clean up XS kit according to section 5.1.

MACHEREY-NAGEL – 03/2021, Rev. 0514
RNA clean up XS
6 Appendix
6.1 Troubleshooting
Problem Possible cause and suggestions
RNA is
degraded / no
RNA obtained
RNase contamination
• Create an RNase-free working environment. Wear gloves during
all steps of the procedure. Change gloves frequently. Use of
sterile, disposable polypropylene tubes is recommended. Keep
tubes closed whenever possible during the preparation. Glassware
should be oven-baked for at least 2 hours at 250 °C before use.
Poor RNA
quality or yield
Reagents not applied or restored properly
• Sample and reagents have not been mixed completely. Always
vortex vigorously after each reagent has been added.
• No ethanol has been added to Clean-up Buffer RCU. Binding of
RNA to the silica membrane is only effective in the presence of
ethanol. Adjust binding conditions by adding ethanol to Clean-up
Buffer RCU Concentrate as described in section 3.
• Store kit components at room temperature (18–25 °C). Storage
at lower temperatures may cause salt precipitation. If precipitation
occurs, incubate the bottle for several minutes at about 30–40 °C
and mix well until the precipitate is redissolved.
• Keep bottles tightly closed in order to prevent evaporation or
contamination.
Ionic strength and pH influence A260 absorption as well as ratio A260 /
A280
• For adsorption measurement, use 5 mM Tris pH 8.5 as diluent.
Please see also:
- Manchester, K L. 1995. Value of A260 / A280 ratios for measurement
of purity of nucleic acids. Biotechniques 19, 208–209.
- Wilfinger, W W, Mackey, K and Chomczyski, P. 1997. Effect of
pH and ionic strength on the spectrophotometric assessment of
nucleic acid purity. Biotechniques 22, 474–481.
Sample material
• Sample material not stored properly. Keep thawed samples on ice
before addition of Buffer RCU.
Contamination
of RNA with
genomic DNA
Sample material already contaminated with DNA
• Digest contaminating DNA in an RNA sample according to section
5.2.

15MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
Problem Possible cause and suggestions
Suboptimal
performance
of RNA in
downstream
experiments
Carry-over of ethanol or salt
• Do not let the flowthrough touch the column outlet after the
second wash using Wash Buffer RA3. Be sure to centrifuge at the
corresponding speed for the respective time in order to remove
ethanolic Wash Buffer RA3 completely.
• Check if Wash Buffer RA3 has been equilibrated to room
temperature before use. Washing at lower temperatures lowers
efficiency of salt removal by Wash Buffer RA3.
• Depending on the robustness of the used RT-PCR system, RT-
PCR might be inhibited if complete eluates are used as template
for RT-PCR. Use less eluate as template.
Store isolated RNA properly
• Eluted RNA should always be kept on ice for optimal stability since
trace contaminations of omnipresent RNases (general lab ware,
fingerprints, dust) will degrade the isolated RNA. For short term
storage freeze at -20 °C, for long term storage freeze at -70 °C.
Higher RNA
yield than
theoretically
possible
• If performing clean up of samples containing less than
approximately 300 ng RNA, subsequent quantification by A260
measurement may simulate yields larger than the RNA input. This
may be due to absorbance of silica abrasion. In order to prevent
incorrect A260 quantification of small RNA amounts, centrifuge the
elution tube for 30 s at 8.000–11.000 x gand withdraw an aliquot
for measurement without disturbing any sediment or use a silica
abrasion insensitive RNA quantification method (e.g., RiboGreen®
fluorescent dye).
Unexpected
A260/A280 ratio
Measurement not in the range of photometer detection limit
• In order to obtain a significant A260/A280 ratio it is necessary that
the initially measured A260 and A280 values are significantly above
the detection limit of the photometer used. An A280 value close to
the background noise of the photometer will cause unexpected
A260/A280 ratios.

MACHEREY-NAGEL – 03/2021, Rev. 0516
RNA clean up XS
6.2 Ordering information
Product REF Pack of
NucleoSpin®RNA Clean-up XS 740903.10
740903.50
740903.250
10 preps
50 preps
250 preps
NucleoSpin®RNA XS 740902.10
740902.50
740902.250
10 preps
50 preps
250 preps
NucleoSpin®RNA 740955.20
740955.50
740955.250
20 preps
50 preps
250 preps
NucleoSpin®RNA Midi 740962.20 20 preps
NucleoSpin®RNA/Protein 740933.10
740933.50
740933.250
10 preps
50 preps
250 preps
NucleoSpin®TriPrep 740966.10
740966.50
740966.250
10 preps
50 preps
250 preps
NucleoSpin®RNA Clean-up 740948.10
740948.50
740948.250
10 preps
50 preps
250 preps
NucleoSpin®miRNA 740971.10
740971.50
740971.250
10 preps
50 preps
250 preps
NucleoSpin®RNA Blood 740200.10
740200.50
10 preps
50 preps
NucleoSpin®RNA Plant 740949.10
740949.50
740949.250
10 preps
50 preps
250 preps
NucleoSpin®FFPE RNA 740969.10
740969.50
740969.250
10 preps
50 preps
250 preps
NucleoSpin®RNA/DNA Buffer Set 740944 Suitable for 100 preps
rDNase Set 740963 1 set
NucleoSpin®Filters 740606 50
Collection Tubes (2 mL) 740600 1000

17MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
6.3 Literature
Fleige S, Pfaffl MW.: RNA integrity and the effect on the real-time qRT-PCR performance.
Mol Aspects Med. 2006 Apr-Jun; 27(2–3):126–39. Epub 2006 Feb 15. Review.
Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, Eveno E, Mueller O,
Schroeder A, Auffray C.: Towards standardization of RNA quality assessment using
user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids
Res. 2005 Mar 30;33(6):e56.
Miller CL, Diglisic S, Leister F, Webster M, Yolken RH.: Evaluating RNA status for
RT-PCR in extracts of postmortem human brain tissue. Biotechniques. 2004 Apr;
36(4):628–33.
Schoor O, Weinschenk T, Hennenlotter J, Corvin S, Stenzl A, Rammensee HG,
Stevanovic S.: Moderate degradation does not preclude microarray analysis of small
amounts of RNA. Biotechniques. 2003 Dec; 35(6):1192–6, 1198–201.
6.4 Product use restriction / warranty
NucleoSpin®RNA Clean-up XS kit components are intended, developed, designed, and sold
FOR RESEARCH PURPOSES ONLY, except, however, any other function of the product
being expressly described in original MACHEREY-NAGEL product leaflets.
MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY!
MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY! MACHEREY-
NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING.
For detailed information please refer to the respective Material Safety Data Sheet of the
product! MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST
ENVIRONMENT. MACHEREY-NAGEL does not assume any responsibility for damages
due to improper application of our products in other fields of application. Application on
the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all
damages resulting from such application.
DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO-
USES ONLY!
ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable for IN-VITRO-
diagnostic use. Please pay attention to the package of the product. IN-VITRO-diagnostic
products are expressly marked as IVD on the packaging.
IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN-VITRO-
DIAGNOSTIC USE!
ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL
USE (INCLUDING, BUT NOT LIMITED TO DIAGNOSTIC, THERAPEUTIC AND/OR
PROGNOSTIC USE).

MACHEREY-NAGEL – 03/2021, Rev. 0518
RNA clean up XS
No claim or representations is intended for its use to identify any specific organism or for
clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or blood banking).
It is rather in the responsibility of the user or - in any case of resale of the products - in the
responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification
products of MACHEREY-NAGEL for a well-defined and specific application.
MACHEREY-NAGEL shall only be responsible for the product specifications and the
performance range of MN products according to the specifications of in-house quality control,
product documentation and marketing material.
This MACHEREY-NAGEL product is shipped with documentation stating specifications
and other technical information. MACHEREY-NAGEL warrants to meet the stated
specifications. MACHEREY-NAGEL´s sole obligation and the customer´s sole remedy is
limited to replacement of products free of charge in the event products fail to perform as
warranted. Supplementary reference is made to the general business terms and conditions
of MACHEREY-NAGEL, which are printed on the price list. Please contact us if you wish to
get an extra copy.
There is no warranty for and MACHEREY-NAGEL is not liable for damages or defects
arising in shipping and handling (transport insurance for customers excluded), or out of
accident or improper or abnormal use of this product; defects in products or components not
manufactured by MACHEREY-NAGEL, or damages resulting from such non-MACHEREY-
NAGEL components or products.
MACHEREY-NAGEL makes no other warranty of any kind whatsoever, and SPECIFICALLY
DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF ANY KIND OR NATURE
WHATSOEVER, DIRECTLY OR INDIRECTLY, EXPRESS OR IMPLIED, INCLUDING,
WITHOUT LIMITATION, AS TO THE SUITABILITY, REPRODUCTIVITY, DURABILITY,
FITNESS FOR A PARTICULAR PURPOSE OR USE, MERCHANTABILITY, CONDITION,
OR ANY OTHER MATTER WITH RESPECT TO MACHEREY-NAGEL PRODUCTS.
In no event shall MACHEREY-NAGEL be liable for claims for any other damages, whether
direct, indirect, incidental, compensatory, foreseeable, consequential, or special (including
but not limited to loss of use, revenue or profit), whether based upon warranty, contract,
tort (including negligence) or strict liability arising in connection with the sale or the failure
of MACHEREY-NAGEL products to perform in accordance with the stated specifications.
This warranty is exclusive and MACHEREY-NAGEL makes no other warranty expressed or
implied.
The warranty provided herein and the data, specifications and descriptions of this
MACHEREY-NAGEL product appearing in MACHEREY-NAGEL published catalogues and
product literature are MACHEREY-NAGEL´s sole representations concerning the product
and warranty. No other statements or representations, written or oral, by MACHEREY-
NAGEL´s employees, agent or representatives, except written statements signed by a duly
authorized officer of MACHEREY-NAGEL are authorized; they should not be relied upon by
the customer and are not a part of the contract of sale or of this warranty.
Product claims are subject to change. Therefore please contact our Technical Service Team
for the most up-to-date information on MACHEREY-NAGEL products. You may also contact
your local distributor for general scientific information. Applications mentioned in MACHEREY-
NAGEL literature are provided for informational purposes only. MACHEREY-NAGEL does
not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using
MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of any
of those applications.

19MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
Last updated: 07 / 2010, Rev. 03
Please contact:
MACHEREY-NAGEL GmbH & Co. KG
Tel.: [phone]
[email protected]
Trademarks:
LightCycler™ is a trademark of a member of the Roche Group
NucleoSpin®is a registered trademark of MACHEREY-NAGEL GmbH & Co KG
RiboGreen®is a registered trademark of Thermo Fisher Scientific
SYBR®is a registered trademark of Molecular Probes, Inc.
All used names and denotations can be brands, trademarks, or registered labels of their respective owner – also if
they are not special denotation.To mention products and brands is only a kind of information (i.e., it does not offend
against trademarks and brands and can not be seen as a kind of recommendation or assessment). Regarding
these products or services we can not grant any guarantees regarding selection, efficiency, or operation.

www.mn-net.com
www.mn-net.com
DE Tel.: [phone] [email protected]
CH Tel.: [phone] [email protected]
FR Tel.: [phone] [email protected]
US Tel.: [phone] [email protected]
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8
52355 Düren · Germany
A035557/0310.3