Macherey-Nagel PF-12 Plus User manual

Manual
PF-12Plus Compact photometer
Photometer PF-12Plus

28 04.07.2014
CONTENT
1. Safety precautions …………………………………………………………………30
1.1 General safety precautions …………………………………………………………30
1.2 Chemical / biological safety …………………………………………………………30
2. Compact photometer PF-12Plus ……………………………………………………31
2.1 Technical data ………………………………………………………………………31
2.2 Design …………………………………………………………………………………32
2.3 Shipment………………………………………………………………………………32
2.4 Interface and power supply …………………………………………………………32
2.5 Operating environment ………………………………………………………………32
3. Usage …………………………………………………………………………………33
4. Setup……………………………………………………………………………………34
4.1 Unpacking the instrument……………………………………………………………34
4.2 Operation with batteries ……………………………………………………………34
4.3 Operation with battery pack …………………………………………………………34
5. Operation ………………………………………………………………………………35
5.1 Keypad ………………………………………………………………………………36
5.2 Configuration …………………………………………………………………………36
5.3 Performance of VISOCOLOR®ECO tests ………………………………………39
5.4 Performance of NANOCOLOR®tube tests ………………………………………40
5.5 Basic photometric functions…………………………………………………………41
6. Memory…………………………………………………………………………………42
6.1 Display memory ………………………………………………………………………42
6.2 Search memory ………………………………………………………………………42
6.3 Delete memory ………………………………………………………………………42
6.4 Output memory ………………………………………………………………………42
7. Special methods ……………………………………………………………………43
7.1 User defined methods ………………………………………………………………43
7.2 Sample numbering …………………………………………………………………44
7.3 Identification of sample location ……………………………………………………44
7.4 Sample dilution ………………………………………………………………………45
7.5 Special methods ……………………………………………………………………45
8. Sample preparation …………………………………………………………………46
9. Procedures for colored or turbid samples ………………………………………47
9.1 Determination of correction values for NANOCOLOR ®tube tests ……………47
10. Cleaning the instrument …………………………………………………………50

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11. Troubleshooting ……………………………………………………………………50
12. Inspection equipment monitoring ………………………………………………50
13. Service ………………………………………………………………………………51
13.1 Spare parts and accessories………………………………………………………51
13.2 Disposal ……………………………………………………………………………51
13.3 Warranty ……………………………………………………………………………51
13.4 Contact ………………………………………………………………………………51

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1. Safety precautions
1.1 General safety precautions
Please read the manual carefully and completely before setting up and using the instru-
ment. Please adhere to all remarks. Not complying with this information may cause harm to
the user or damage the instrument. In order not to compromise the instrument`s safety
measures, it may never be installed or used in any other way than described in this manual.
1.2 Chemical / biological safety
When using the instrument, it may be necessary to handle hazardous chemicals or biologi-
cally harmful samples. Please adhere to the following guidelines when using the instrument:
• Please read all information concerning hazards and safety measures in the material
safety data sheets and on the packages of the compounds you work with.
• All used up compounds need to be disposed of in agreement with national rules and
regulations.
• The type of safety equipment needs to be in line with the concentration and hazard
potential of the given substance used.

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2. Compact photometer PF-12Plus
2.1 Technical data
Type: Single-beam filter photometer with microprocessor control,
self test and auto calibration
Wavelength range: 340–860 nm
Optics: Automatic filter wheel with 7 interference filters, insensitive to
external light for fast measurements without cuvette slot cover
Wavelengths: 345 / 436 / 470 / 540 / 585 / 620 / 690 nm plus 1
compartment
for an additional filter
860 nm LED for NTU-Measurement
Wavelength accuracy: ± 2 nm
Bandwidth at half of maximum transmission 10–12 nm
Light source: Xenon lamp
Detector: Silicon-photodiode
Blank value: Automatic
Measuring modes: Over 100 preprogrammed tests (NANOCOLOR®tube tests
and VISOCOLOR®ECO tests), absorbance, transmission,
factor, standard, turbidity, 50 freely programmable methods
Photometric range: ± 3 E
Photometric accuracy: ± 1 %
Stability: < 0.002 E/h
Cuvette holder: Round tubes 16 mm OD Data
memory: 1000 results, GLP-compatible
Display: Backlit graphic display, 64 x 128 pixels, 12 languages; All
important data at a glance: result with measuring unit, date,
time, sample number, sample location, dilution
Operation: Intuitive menu navigation, durable keypad, test selection via
entering the test number or from parameter lists
Quality control: With NANOCONTROL NANOCHECK
Interfaces: USB B 2.0
Update: Free of charge via Internet / PC
Operating range: 0–50 °C, up to 90 % relative humidity
Power supply: USB-power supply, disposable or rechargeable batteries
Housing: Water- and dustproof, IP 68
Dimensions: 215 x 100 x 65 mm
Weight: 0.7 kg
Warranty: 2 years
This device complies with the following directives:
2006/95/EC Low Voltage Directive
2004/108/EC EMC Directive

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2.2 Design
1. Graphic display 128 x 64 Pixels
2. Cuvette slot for tubes with 16 mm outer diameter
3. Keypad
4. USB-interface (2.0) for connection of power sup-
ply, charging the optional battery pack, data trans-
fer and update
2.3 Shipment
If one of the components from the following list should be defective or missing, please con-
tact MACHEREY-NAGEL or your local distributor.
Compact photometer PF-12Plus
incl.: Software DVD, manual, 4 AA Mignon batteries, 4 empty cuvettes, funnel, beaker,
syringe, USB-cable, calibration cuvette and certificate in a rugged case
2.4 Interface and power supply
USB-interface for:
• Update via Internet
• Data export to PC
• Power supply via PC USB-interface
• External power supply (REF 919 220) with USB-interface
Additional power supply:
• BatteriesAA/ Mignon
• 4 rechargeable batteries (REF 919 221), rechargeable with additional charger
• Battery pack (REF 919 201), rechargeable via USB
2.5 Operating environment
The following conditions are necessary to provide longevity and flawless functioning of the
instrument.
• Operating temperature 0–50 °C
• Relative humidity: up to 90 %
• To ensure safe results, the instrument should not be stored in extremely dusty, damp
or wet conditions (the instrument optics are IP 68 proof and don’t experience any
sustained damage from such conditions).
Keep the instrument surface, cuvette slot and any accessories clean and dry. Splashes or
spilled substances should be wiped away and cleaned immediately (see 10. Cleaning the
instrument, page 50).
Important note: Protect the instrument from extreme temperatures (e.g. radiators, direct sun
light and other heat sources).
PF-12Plus

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3. Usage
As part of GLP, please familiarize yourself with all reagents used in your application. Read
all product labels and MSDS before working with chemicals.
To ensure proper functioning of all inbuilt safety measures, the instrument may, under no
circumstances, be used or installed in a different way than stated in the manual.
Only use the instrument as instructed to analyze MACHEREY-NAGEL tests. Please do not
open the instrument or make any other unauthorized modifications. The instrument is a
highly sensitive measuring device with high accuracy for the photometric evaluation of
MACHEREY-NAGEL tests. Unauthorized modifications or opening the instrument in an inap-
propriate way can result in maladjustments of the instrument’s optical components, or similar
damage, which in turn can lead to wrong results.
Note: Due to the reasons stated above, MACHEREY-NAGEL declines any responsibility for
the correct functioning of the instrument if it has been opened or used improperly and objects
to any warranty claims made in such a case. MACHEREY-NAGEL’s warranty and liability
obligations as to product(s) or to any party on account thereof shall be void and of no effect
if the product(s) or any part thereof have been subject to unauthorized change, abuse,
misuse, accident or alteration, unauthorized application or installation not in compliance with
the pro- duct(s) specifications as set forth in the product leaflets / instructions hereof
(“unauthorized action”) and such unauthorized.

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4. Setup
4.1 Unpacking the instrument
Take the instrument out of the box and check for damage caused during shipment.
4.2 Operation with batteries
The PF-12Plus photometer runs on 4 AA Mignon batteries (included in delivery). Recharge-
able batteries, e.g. 4 NiMH AA 2500 mAh can be used in the instrument and are available as
accessories (REF 919 221). The charger and rechargeable batteries need to be ordered
separately. The battery status is displayed in the upper part of the display after switching on
the device. When connected to an USB adapter, a “U” is displayed besides the battery symbol.
When changing batteries, we recommend to always exchange the entire set. The battery
compartment is at the bottom of the instrument. To change the batteries, the cuvette slot has
to be empty.
1. Turn the instrument around and place it on a soft surface.
2. Unscrew the screws of the battery compartment and take off the lid.
3. Insert the 4 batteries in the respective slots. Make sure polarity of batteries and slots match.
4. Put the lid back on, tighten the screws and turn the instrument around.
4.3 Operation with battery pack
The PF-12Plus can be equipped with an internal battery pack, which can be charged via the
USB port. The charging status of the internal battery pack is displayed in the upper part of
the display in form of a small battery symbol with an “A”. When connected to a USB charger
additionally a “L” is going to be displayed besides the battery symbol, indicating the ongoing
charging process. You can order such a power pack separately and insert it into the instru-
ment. Detailed installation instructions come with the battery pack.

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5. Operation
The PF-12Plus is designed for easy handling and operation. The photome-
ter PF-12Plus has a 20-part keypad: 10 numbered keys and 10 keys with
special functions.
Date
Additional information
Selected sub-method
Selection of sub-method
Sample location
Sample number
Time
Method number and title
Measurement value with unit and suffix
20–80 % range
Dilution
Zero and measurement
The display is devided into three sections. In the upper part date, battery status and time are
shown. Depending on the mode, in the middle section the actual measurement values, menu
options or respective menu symbols are displayed. The lower part of the display is a task bar
showing the possible options in the current mode.
Press to return to the next higher level. Press repeatedly to return to the method se-
lection mode.
PF-12Plus

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5.1 Keypad
Key : On / Off switch
Keys ... : For entering digits, letters, special characters
Keys / / : Double-function keys, for sample number, location, dilution
Key : For entering decimal points, to select special method
Key : Abort, return to method selection
Key : Scroll up
Key : Scroll down
Key : Enter, call up last result
Key : Memory, call-up memory functions, store / not-store results
Key : Absorbance program, recall the absorbance after measure-
ment, estimated value and NTU value after measurement / se-
lection of prefix
Key : Manuell zero adjustment
Key : Measurement, consecutive measurements are numbered con-
tinuously, call up last test
5.2 Configuration
To get to the configuration mode enter “900” in the method selection mode
or use / to select the entry “Settings” in the list and press
to confirm.
Additionally there is a quick start option. Every setting has its
own three-digit number which can be entered in the start menu
to directly access each menu.
The configuration menu is based on icons. You can navigate through the menu via / ,
confirm by pressing and follow the instructions on the display.
5.2.1 Language (Method 954)
Select the desired language via / and press to confirm.
5.2.2 Time (Method 951)
Select the digits via / and adjust via keypad. Press to confirm. Select the
desired time format via / and press to confirm.