MRC UT-FLU1 Series User manual

Operation Manual
UT-FLU1 SERIES
Portable Fluorometer
PLEASE READ THIS MANUAL CAREFULLYBEFORE OPERATION
Hagavish st. Israel 58817 Tel: [phone], Fax: [phone] [email protected]
MRC.4.22

I
Foreword
Thank you for purchasing our Fluorometer. This user manual describes how the instrument
works and the operation guide, please read carefully before operating it. Keep user manual
for later use in case you meet with difficulties.
Opening Check
Please check the instruments as well as all accessories with packing list when you first open
it. If you find anything wrong or missing, please contact distributor or manufacturer.

II
Safety and Operating Precautions
1. Safety Information
To assure the safe operation, please read this manual carefully before operating.
2. Operating Precautions
Operation, maintenance and repair of the instrument should comply with the basic
guidelines and remarked warnings below.Otherwise,warranty and working life of the
instrument may be influenced.
NOTICE: Operation before reading the Manual is forbidden,otherwise
instrument in operating may cause injury or electric shock. Read the guidelines
and directions below and carry out countermeasures according to them.
This instrument conform to I class B type common equipment of standard
GB9706.1. Indoor use only.
Read this manual carefully before operating the instrument. Only the qualified
person who have the skill or experience can operate it.
Disassemble the instrument without permission is forbidden,except the parts
that could be disassembled in the manual. Or you will lose the warranty
qualification,and may have the risk of electric shock.
Do not cover anything on power wire in operating. Do not put power wire in
the place where personnel ambulates. Insert and pull plug with hand.Make sure
plug insert to jack completely.
For Research Use Only.This instruments is not a medical device and
is not intended to be used for clinical test.
CAUTION: Biological Contamination
All test sample,quality control sample,calibration sample,and components
contaminated by these sample are deemed to be infectious. Please wear the
gloves before touching.

III
Power off when you finish your work. Pull off the connector plug if the
instrument stopped working ,and make sure it covered with a cloth or plastic
paper to prevent from dust.
Pull off the connector plug immediately in following cases, and contact
supplier or skilled maintenance to manage:
There is some liquid flowing into the Instrument.
Drenched or fire burned.
Instrument dropping or outer shell damaged.
Malfunction.
The Instrument should be put in dry place, less dust, far away from water and
high-intensity light. What’s more, the place should be well ventilated, no
corrosive gas or strong magnetic interference, far away from heater, stove or
any other heat.

Portable Fluorometer Operation Manual Contents
IV
Contents
Contents..................................................................................................................................IV
Chapter 1 Introduction....................................................................................................... 1
Chapter 2 Specifications..................................................................................................... 2
Chapter 3 Instrument Structure........................................................................................ 3
Chapter 4 Installation......................................................................................................... 4
4.1 Opening Check........................................................................................................... 4
4.2 Installation Conditions............................................................................................... 4
4.3 Installation Steps........................................................................................................ 4
4.4 Operating Considerations........................................................................................... 4
Chapter 5 Software Introduction.......................................................................................6
5.1 Self Testing.................................................................................................................6
5.2 Home Interface........................................................................................................... 6
5.2.1 Fluorescence Detection................................................................................... 7
5.2.2 dsDNA Detection............................................................................................ 9
5.3 Report....................................................................................................................... 17
5.4 Settings..................................................................................................................... 19
5.4.1 Time...............................................................................................................19
5.4.2 LCD............................................................................................................... 20
5.4.3 Print............................................................................................................... 21
5.4.4 Updata........................................................................................................... 22
Chapter 6 Maintenance, Storage and Transportation................................................... 23
Chapter 7 Fault Analysis and Treatment........................................................................ 24
Chapter 8 Accessories........................................................................................................25

Portable Fluorometer Operation Manual Chapter 1 Introduction
1
Chapter 1 Introduction
Fluoroimmunoassay (FIA) technology has advantages of high specificity, sensitivity and
utility. Therefore, it is used for detecting biological active compounds of low concentration,
such as protein (enzyme,receptosome,antibody), nucleic acid, hormone (steroid, thyroid
hormones), medicine and microorganism.
UT-FLU1 fluorometer is based on fluoroimmunoassay to detect the luminous intensity of
fluorescent reagent in immunoassay (IA). Under the condition of low concentration, the
sample concentration is linear to fluorescence intensity. Thus, the testing sample can be
analyzed in qualitative and quantitative analysis

Portable Fluorometer Operation Manual Chapter 2 Specifications
2
Chapter 2 Specifications
Normal Operating Conditions
Ambient temperature：4C45C
Relative humidity：≤70%
INPUT Voltage：DC 6~35 V
Basic Parameters and Performance
Models and Specification
Model Description Light sources Excitation wavelength Emission wavelength
UT-FLU1AFlurometer UV LED 365±20nm 420-480nm (60nm)
Blue LED 460±20nm 525–570nm (45nm)
UT-FLU1BFlurometer Blue LED 460±20nm 525–570nm (45nm)
Red LED 625±20nm 670–725nm (55nm)
UT-FLU1CFlurometer Blue LED 460±20nm 525–570nm (45nm)
Green LED 525±20nm 575–640nm (65nm)
Model
Parameters
UT-FLU1A、UT-FLU1B、UT-FLU1C
Light source Monochromatic LED
Power < 5 W
Linearity 2
R
>0.995
Repeatability <1.5%
Stability <1.5%
Sensitivity Ds DNA：1ng/ml；
Assay speed 3s（single-pass）
Dimension (W*D*H) 194*155*72.5 mm
N.W. (kg) 0.4Kg

Portable Fluorometer Operation Manual Chapter 3 Instrument Structure
3
Chapter 3 Instrument Structure
This chapter introduces the structure of instrument.If it is used for the first time, please read
this chapter carefully to make a better preparation.
Structure 1：
Fig3.1 Structure1
Structure 2：
Fig3.2 Structure2
Lid
Printer port
USB port
Power socket
PCR tube base/Sample
chamber
Display Screen
Switch

Portable Fluorometer Operation Manual Chapter 4 Installation
Chapter 4 Installation
Opening Check 4.1
Each UT-FLU1 has been inspected strictly before packing and transportation. Please check
again when you receive it.Contact with your local distributor or manufacturer in case of
The package inverted or deformated
The package has an obvious stains of water
The package has marks of impact
The package has been opened
Please check instrument and accessories if package is sound and intact.
Check all accessories according to the packing list
Check instrument appearance if there is cracks,damage or deformation
4.2 Installation Conditions
Put on the dry, clean and horizontal worktable
Working conditions:
Clean air without corrosion steam or dust
Temperature between +10°C to +40°C
4.3 Installation Steps
1. Take out the instrument on the worktable slowly and gently.
2. Switch off the instruments with the button on the back of instrument. Take out the 12V 1A
power adapter and plug it into the socket on the back of instrument. Then connect another
side to AC100~200V power supply.
3. Instrument will start to self-testing after power on, then get ready for assay.
4.4 Operating Considerations
Cuvettes, 0.2ml fluorescence PCR tube and 0.5ml fluorescence PCR tube can be used for
sample test on UT-FLU1
4

Portable Fluorometer Operation Manual Chapter 4 Installation
5
NOTE: Fluorescence PCR tube should be used with tube base together.
Sample test steps：
1. Insert PCR tube base first, as fig4.1.
2. Insert fluorescence PCR tube into tube base.
3. Close lid;Click “detecting” for testing.
Fig4.1 Sample Test Steps
To insure the effect of test results,volume of sample should be 3ml for cuvette,o.1ml for
0.2ml fluorescence PCR tube,and 0.2ml for 0.5ml fluorescence PCR tube.
Please use corresponding solvent diluting sample to reach the require volume if sample is
not enough. Pay attention to that concentration range should be within the limit of detection,
and the sample volume should be equal to volume of standard curve.
Fluorescence PCR tube
PCR tube base