Sibata 080150-076 User manual

CODE080150-076
DNPH Active Gas Tube
(for Aldehydes and Ketones)
Thank you for purchasing this product.
zThis operation manual describes
important precautions for preventing
accidents as well as procedures for
handling the product.
zTo ensure safety, read this operation
manual thoroughly before use and
use the product correctly.
zAfter reading this operation manual,
keep it handy for future reference.
2005-2016 SIBATA SCIENTIFIC TECHNOLOGY LTD.

2
Table of Contents
Page Page
Before Use Main Specifications
zAbout This Operation Manual Optional Products
zIncluded Items Product Shelf Life
Safety Precautions Product Disposal
About This Product Inquiries
Names of Parts
Instructions for Use
zSampling
Analysis Procedure
zPreparing Standard Solutions
zPreparing Calibration Curves
zAnalyzing Samples
zCalculating Concentrations
zCalculation Method in Japan's Ministry of Health, Labour and Welfare
Guidelines (for reference)
Be Sure to Read This
Page 4 lists important precautions for ensuring the safe use of the
product, and for preventing harm to you and other people and damage
to property. Be sure to thoroughly read these precautions before use
and observe them.

3
Before Use
WARNING
zRead and understand the instructions
given in this operation manual before
using the product.
zKeep this operation manual handy for
future reference.
zFamiliarize yourself with the safety
precautions given in this operation
manual and observe them.
zObserve usage procedures that are
suitable for the product and are
specified in this operation manual.
Be sure to observe the above instructions.
Failure to do so may result in an accident or injury.
zAbout This Operation Manual
zThe information in this operation manual is subject to change without
notice in the interest of product improvement.
zEvery effort has been made to ensure that the information in this
operation manual is correct. If you discover any errors or omissions,
however, please contact your Sibata representative.
zThe copyright of this operation manual belongs to Sibata Scientific
Technology Ltd. This operation manual, or any part thereof, may not
be reproduced, reprinted, or altered in any form without prior written
permission from Sibata Scientific Technology Ltd.
zIncluded Items
Check the contents of the package before using the product.
zDNPH active gas tubes························· Set of 10
(individually packaged in aluminum bags)
zOperation manual (this manual)·············· 1 copy
zInspection report ································· 1 copy
Note: If any of the items are damaged or missing, contact your Sibata representative
or the vendor where you purchased the product.
Note: The product does not include a suction pump or tubing for connections. Please
obtain these items separately.

4
WARNING
CAUTION
Do Not Touch
Disconnect
Power Plug
Safety Precautions (Be Sure to Read This)
The precautionary information in this operation manual is provided to
ensure the safe use of the product and to prevent property damage
and injury to you and other people.
It is all important for ensuring safety, so be sure to read it thoroughly
before using the product and observe it during use.
zSymbols for Safety Information
Various symbols are used in this operation manual to indicate
warnings and instructions. The meanings of the symbols are as
follows. Fully understand the following descriptions before reading
the subsequent sections.
Indicates a potentially hazardous situation
which, if not avoided, could result in serious
injury or possibly death.
Indicates a potentially hazardous situation
which, if not avoided, may result in minor to
moderate injury or equipment damage.
Examples of Symbols
indicates a prohibited action (which must not be
done). Specific details are given in or near the
symbol. The symbol shown on the left indicates that
touching is prohibited.
indicates a mandatory action (which must be
done). Specific details are given in or near the
symbol. The symbol shown on the left indicates that
the power cord should be unplugged from the power
outlet.

5
zPrecautionary Information
Prohibited
Do not modify the
product or use it for
purposes other than
chemical analysis.
Mandatory
Analysis should only
be performed by a
person with technical
expertise about
chemical analysis.
Doing so may result in an
accident.
Not doing so could result in
an accident.
Mandatory
For hazardous or
highly volatile
substances, wear
protective gear and
take measures to
prevent fires.
Prohibited
Do not put DNPH
active gas tubes in
your mouth.
Not doing so could result in
accidents or fires.
Doing so could cause
injuries or other accidents.
Mandatory
Be sure to keep the
product in a location
at 4 °C or below.
Mandatory
When finished using
the product, dispose
of it in accordance
with instructions
given by local
authorities.
Not doing so could cause
deterioration in product
performance or other
aspects.
Not doing so could cause
environmental pollution or
other problems.
Also read and understand other warnings and instructions
indicated elsewhere in the manual before using the product.
WARNING
CAUTION

6
About This Product
The DNPH (2,4-dinitrophenylhydrazine) active gas tube is a sampler
capable of determining the concentration of aldehydes and ketones in the
environment, typified by formaldehyde (HCHO), by using a pump to
suction them from the atmosphere.
The sampler immobilizes the airborne aldehydes and ketones by putting
them in contact with silica gel impregnated with
2,4-dinitrophenylhydrazine (hereinafter "DNPH") in a polyethylene tube.
Then after extracting the aldehydes and ketones with acetonitrile, they
are measured by high-performance liquid chromatography (hereinafter
"HPLC").
The product is best suited for use in measuring indoor environments.
Names of Parts
zDNPH Active Gas Tube
a: Polyethylene plug b: Polyethylene tube
c: Polyethylene filter d: Silica gel impregnated with DNPH
e: Suction port f: Polyethylene cap

7
Instructions for Use
DNPH active gas tubes must be stored at low temperatures.
Therefore, be sure to take measures to keep them cool while
carrying them. If transporting them, make sure they are kept at
4 °C or below during transport.
zSampling (adsorption in the active gas tube)
(1) Cut open the aluminum bag with scissors or something similar.
(Markings on the aluminum bag indicate where to cut.) The bag
contains the DNPH active gas tubes individually packaged in aluminum.
Keep any bags not immediately used for sampling stored in a location
at 4 °C or below.
(2) Just before sampling, cut the small aluminum bag at the cut mark
indicated with an arrow (can be cut by hand). Then open the zip lock
seal and remove the DNPH active gas tube.
Note: The small aluminum bag includes a notation area for recording the measurement
point or other information. When writing down, use a pencil, which has little effect
on measurements, and avoid using an ink pen.
(3) Remove the polyethylene plug
and cap from the DNPH active
gas tube and use Toalon tubing
(flexible PVC tubing) or other
means to connect the active gas
tube to the pump. Then start
sampling. Perform measurements
in accordance with laws and
regulations applicable for given
measurement objectives.
Note: The outer diameter of the suction
end of the DNPH active gas tube is
about 6 mm.
Note: To measure concentrations in
outdoor air environments, Sibata
recommends using a separately sold
ozone scrubber connected to the end
of the DNPH active gas tube to avoid
the influence of ozone.
(4) After exposure, promptly reinstall the polyethylene plug and cap on the
DNPH active gas tube, put the assembly back into the small aluminum
bag, and seal the zip lock closure.
Then keep it stored in a location at 4 °C or below.
DNPH active
gas tube
Tube
Pump

8
Analysis Procedure
WARNING
Extraction and analysis requires technical expertise
regarding chemical analysis.
Inexperienced operators should always perform
extraction and analysis work under the supervision
of an experienced operator or outsource the process
to an analysis and measurement service provider
qualified to do so. In addition, precautionary
measures, such as wearing protective gear or
preventing fires, are required for handling the
hazardous or highly volatile substances described
below. Therefore, obtain and reference an SDS or
other relevant safety information in advance.
zPreparing Standard Solutions
WARNING
This product is designed for use with hazardous
and highly volatile substances. Implement
appropriate precautionary measures.
Prepare standard solutions by diluting commercial DNPH derivatives of
aldehydes and ketones with acetonitrile.
Formaldehyde (4 μg HCHO/mL)
Place 1 mL of the formaldehyde DNPH standard solution (40 μg
HCHO/mL from Wako Pure Chemical Industries) in a volumetric flask.
Add and dissolve acetonitrile to make a total volume of 10 mL.
zPreparing Calibration Curves
WARNING
This product is designed for use with hazardous
and highly volatile substances. Implement
appropriate precautionary measures.
Prepare a series of about five dilution levels from each standard solution.
Inject the series of standard solution dilutions into the HPLC system and
measure the peak area (or peak height) of target substances.
Repeat this process to determine a mean value.
Calculate the slope and intercept of the regression line from the
concentrations of the solutions corresponding to the chromatogram peak
height or peak area values using the least squares method or some
Example

9
other means. At this point, the indicated coefficient of correlation for the
two variables must be close to 1.
Example of HPLC Analytical Conditions
Measurement wavelength : 360 nm
Mobile phase : Acetonitrile/water (60/40)
Flowrate : 1.0 mL/min
zAnalyzing Samples
WARNING
This product is designed for use with hazardous
and highly volatile substances. Implement
appropriate precautionary measures.
In a clean location, remove the
DNPH active gas tube used for
sampling from its aluminum bag.
Remove the polyethylene plug and
cap from the gas tube, attach a
10 mL glass syringe to the tube,
and slowly inject 5 mL of
acetonitrile. Add additional
acetonitrile to make 5 mL for use as
the sample solution.
Analyze the sample solution in the same manner as described in
"Preparing Calibration Curves" to determine the quantities of target
substances collected based on the calibration curve.
zCalculating Concentrations
Calculate the concentrations of target substances being measured using
the following formula.
Calculating Collection Quantities
Formula 1
zV : Collected volume (L) at converted temperature
zV' : Integrated volume (L) during sampling
zT : Temperature (°C) to be converted
zt : Mean air temperature (°C) during sample acquisition, or mean water temperature
(°C) of the integrating flow meter (if using a wet-gas type integrating flow meter)
Syringe

10
Calculating Concentrations
Formula 2
zC = Concentration (μg/m3) of aldehydes and ketones in the sampled air at the
converted temperature
zWa = Quantity (ng) of aldehydes and ketones collected in one active gas tube
zWb = Blank value (ng)
zV= Collected volume at converted temperature (L)
zCalculation Method in Japan's Ministry of
Health, Labour and Welfare Guidelines (for reference)
The calculation method indicated in the Ministry of Health, Labour and
Welfare guidelines (Committee on Sick House Syndrome: Indoor Air
Pollution) is shown below.
The method involves graphing the relationship between the injected
quantity of aldehydes and the corresponding peak area or peak height
values and then using that graph as a calibration curve.
The following formula is recommended for calculations if results are to
be compared to indoor air quality guideline values indicated by the
Ministry of Health, Labour and Welfare.
Calculation Formula
Formula 3
zC = Concentration (μg/m3) of aldehydes in the sampled air at 20 °C
zAs = Mass (μg) of aldehydes in the sample solution, as determined from the
calibration curve
zA = Mass (μg) of aldehydes in the blank test solution, as determined from the
calibration curve
zD = Dilution factor
zE = Volume (μL) of sample solution
zv = Volume (μL) injected into the HPLC system
zV = Volume (L) of sample air collected, as measured by a gas meter
zt = Mean air temperature (°C) during sample acquisition, or mean water temperature
(°C) of the integrating flow meter (if using a wet-gas type integrating flow meter)
zp = Mean atmospheric pressure (kPa) during sample acquisition
Use (P - Pw) if using a wet-gas type integrating flow meter. P is the standard
atmospheric pressure (101.3 kPa), whereas Pw is the saturated vapor pressure
(kPa) at t, the mean air temperature during sample acquisition.

11
Main Specifications
Adsorbent Silica gel impregnated with 2,4-dinitrophenylhydrazine
(350 mg per tube)
Size About 16 mm dia. ×43 mm
Weight About 3 g
Tubes Included Set of 10 (individually packaged in aluminum bags)
Product Code 080150-076
Optional Products
Product Code Product Name
080150-0761 Ozone scrubbers (set of 10)
080860-034 Mini pump MP-Σ30HNⅡ
080860-104 Mini pump MP-Σ100HNⅡ
020060-5A Volumetric flasks, super grade, 5 mL (set of 10)
Product Shelf Life
The shelf life of the product is indicated on the outer
packaging. Confirm that the product is still within its useful
shelf life.
Product Disposal
After using gas tubes, reference the part materials indicated in
"Names of Parts" on page 6 and dispose of the tubes in
accordance with instructions given by local authorities.
Note: After extraction, dry the silica gel impregnated with DNPH and
dispose of it as silica gel.

Inquiries
If you have any questions about this product, or if there is any
other way in which we can be of assistance, contact your Sibata
representative or the vendor where you purchased the product.
Please also visit our website to contact us for inquiries.
2016.10.28H(13)