Thermo Scientific 314 User manual

Lab-Line Air-Jacketed Compact
Automatic CO2Incubators
Model No.
314
314-1CE
LT1447X1 • 4/11/07

Safety Information ........................................................................................................................................................3
Alert Signals ..........................................................................................................................................................3
Specifications ..............................................................................................................................................................4
Electrical Requirements ........................................................................................................................................4
Temperature Range ..............................................................................................................................................4
Temperature Control ..............................................................................................................................................4
Uniformity in Chamber ..........................................................................................................................................4
Algorithm................................................................................................................................................................4
Carbon Dioxide Tension ........................................................................................................................................4
Volume ..................................................................................................................................................................4
Overall Dimensions................................................................................................................................................4
Chamber Dimensions ............................................................................................................................................4
Shelf Dimensions ..................................................................................................................................................4
Shipping Weight ....................................................................................................................................................4
Declaration of Conformity ......................................................................................................................................5
Environmental Conditions......................................................................................................................................5
Unpacking and Installation ..........................................................................................................................................6
Shipping Carton ....................................................................................................................................................6
Location ................................................................................................................................................................6
Electrical Requirements ........................................................................................................................................6
Humidification, 314 Series Automatic CO2 Incubators ........................................................................................7
Gas Filter Interface Assembly Kit ..........................................................................................................................7
Installation Procedure............................................................................................................................................7
Figure A: Back of Unit - Rear Panel View, Exterior ..............................................................................................8
Description and Features ..........................................................................................................................................10
Basic Components ..............................................................................................................................................10
Description ..........................................................................................................................................................12
Features ..............................................................................................................................................................13
Double-Door Construction with Inner Door Defogger ..................................................................................13
Control Panel ......................................................................................................................................................13
Proper Sequencing Important in Operating Command Keys ..............................................................................16
Alarms..................................................................................................................................................................17
Password ............................................................................................................................................................17
Additional Features..............................................................................................................................................18
Operation....................................................................................................................................................................19
Startup and Operation ........................................................................................................................................19
Password Feature ................................................................................................................................................19
Setting Operating Temperature and High Limit Setpoint ....................................................................................20
Condensation on Inner Glass Door ....................................................................................................................21
Temperature Calibration ......................................................................................................................................22
CO2 Calibration ..................................................................................................................................................22
Using Fyrite ................................................................................................................................................................24
Taking a Fyrite Reading ......................................................................................................................................25
Maintenance ..............................................................................................................................................................28
Cleaning ..............................................................................................................................................................28
Care and Cleaning of Stainless Steel..................................................................................................................28
The Alloy Called Stainless ..................................................................................................................................28
Stainless Guidelines ............................................................................................................................................28
The pH Factor ......................................................................................................................................................29
Special Considerations ........................................................................................................................................29
Cleansing Agents ................................................................................................................................................30
Cleaning Methods ........................................................................................................................................30
Materials Effective in Disinfecting ................................................................................................................31
Unit Repairs ........................................................................................................................................................31
Replacement Parts ....................................................................................................................................................32
Ordering Procedures ..................................................................................................................................................33
Decontamination Statement ......................................................................................................................................34
Two Year Limited Warranty ........................................................................................................................................36
2
Ta ble of Contents

3
Your Thermo Scientific Lab-Line Air-Jacketed Compact
Automatic CO2 Incubator has been designed with func-
tion, reliability, and safety in mind. It is your responsibility
to install it in conformance with local electrical codes. It is
most important that the user follow installation instructions
exactly as written. Failure to do so is likely to lead to
improper operation, erroneous calibrations and possible
damage to the equipment. Do not attempt operation with-
out this information.
The following equipment is to be supplied by the user:
• Dry CO2 gas (research grade or better).
• A dual-stage regulator for the CO2 tank.
• 1/4" (0.64 cm) ID Flexible tubing (appropriate
length from tank to incubators), and connected
per local codes.
• Fyrite or other CO2 analyzer.
BE ADVISED: INSTALLATION AND PRE-OPERATION
PROCEDURE WILL TAKE AT LEAST 24 HOURS TO
COMPLETE. DO NOT ATTEMPT TO RUSH THE
PROCESS WITH SHORT CUTS TO THE PROCEDURES
DESCRIBED IN THIS MANUAL.
Safety Information
Warning
Warnings alert you to a possibility of
personal injury.
Caution
Cautions alert you to a possibility of
damage to the equipment.
Note
Notes alert you to pertinent facts and
conditions.
Hot Surface
Hot surfaces alert you to a possibility of
personal injury if you come in contact
with a surface during use or for a period
of time after use.
Alert Signals

4
Electrical Requirements
314: 120 Volts, 50/60 Hz, 200 Watts, 1.7 Amps
314-1CE: 230 Volts, 50/60 Hz, 200 Watts, 0.8 Amps
Temperature Range
Ambient +5°C to 60°C
Temperature Control
±0.1°C at 37°C
Uniformity in Chamber
Within the chamber, ±0.25°C
Algorithm
Temperature: PID
CO2: SmartGas®
Carbon Dioxide Tension
Range: 0% to 20%
Control: ±0.1%
Volume
1.2 cu. ft. (33.9 liters)
Overall Dimensions
22.5”W x 18”D x 19”H (57cm x 46cm x 48cm)
Chamber Dimensions
14”W x 12”D x 12”H (36cm x 31cm x 31cm)
Shelf Dimensions
2 standard; space for 3 additional shelves.
13-3/8” (tapering back to) 12-1/8" W x 11-1/2" D (34~30.7 x 29.2 cm)
Shipping Weight
70 lbs. (32 kg)
Specifications

Declaration of Conformity
(for 220-240 volt, -33 CE models only)
We hereby declare under our sole responsibility that this product conforms with the technical requirements of
the following standards:
EMC: EN 61000-3-2 Limits for harmonic current emissions
EN 61000-3-3 Limits for voltage fluctuations and flicker
EN 61326-1 Electrical equipment for measurement, control, and
laboratory use; Part I: General Requirements
Safety: EN 61010-1 Safety requirements for electrical equipment for
measurement, control, and laboratory use;
Part I: General Requirements
EN 61010-2-010 Part II: Particular requirements for laboratory equipment for
the heating of materials
per the provisions of the Electromagnetic Compatibility Directive 89/336/EEC, as amended by 92/31/EEC and
93/68/EEC, and per the provisions of the Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC.
The authorized representative located within the European Community is:
Thermo Fisher Scientific
419 Sutton Road
Southend On Sea
Essex SS2 5PH
United Kingdom
Copies of the Declaration of Conformity are available upon request.
Environmental Conditions
Operating: 17°C to 27°C; 20% to 80% relative humidity, non-condensing.
Installation category II (overvoltage) in accordance with IEC 664.
Pollution degree 2 in accordance with IEC 664.
Altitude Limit: 2,000 meters.
Storage: -25°C to 65°C
10% to 85% relative humidity
5
SPECIFICATIONS

6
Shipping Carton
The shipping carton should be inspected upon delivery.
When received, carefully examine for any shipping dam-
age before unpacking. If damage is discovered, the deliv-
ering carrier should both specify and sign for the damage
on your copy of the delivery receipt.
Open the carton carefully making certain that all parts are
accounted for before packaging materials are discarded.
After unpacking, if damage is found promptly report it to
the carrier and request a damage inspection promptly.
IMPORTANT: Failure to request an inspection of damage
within a few days after receipt of shipment absolves the
carrier from any liability for damage. You must call for a
damage inspection promptly.
Location
Place the unit where it will be used, away from external
vibration sources, drafts and wide variations in ambient
temperature. To prevent damaging the tubing, allow at
least 3” (8 cm) between the back of the incubator and
wall. Choose a location near a power supply that match-
es the unit nameplate requirements.
Electrical Requirements
120 VAC models require a 120 VAC, 50/60 Hz power
source. They are supplied with a 3-wire line cord. It
should be plugged into an outlet designed for 3-prong
plugs. If an extension cord is used, it also should be the
3-wire grounded type. For an outlet designed to accept 2-
prong plugs (ungrounded), it is required that a qualified
electrician replace the outlet with a new grounded type.
230 VAC models require a 230 VAC, 50/60 Hz power
source. The user must install a plug to conform with local
code and configuration requirements.
Unpacking and Installation
Caution
This unit is designed for use with CO2
only. Do not use with any other gases.
Failure to observe these precautions
can result in explosion and/or fire and
serious injury and/or death and proper-
ty damage.
Note
Leave unit disconnected when not in
use.

7
Humidification, 314 Series
Automatic CO2 Incubators
The chamber is designed in such a way that a cold spot is
formed causing moisture that is present in the chamber
atmosphere to condensate. The condensation is diverted
into a humidity trough that is attached to the rear wall of the
chamber. Distilled water is not necessary to add since this
water will be re-circulated throughout the chamber.
Gas Filter Interface Assembly Kit
Models 314 and 314-1CE require this assembly to be used
for both CO2 and air.
Kit includes:
• (4) Hose Clamps - #250-331-00
• (1) In-Line Filter - #525-034-00
• (1) 10 ft. (4.57 m) length of 1/4” (6.35 mm) ID
PVC tubing - #720-418-00
Installation Procedure
See next page for illustration.
1. Turn the unit power switch to the OFF position.
2. Connect tubing to tank gas outlet.
3. Connect 1/4” (0.64 cm) ID tubing to hose barb on
one side of in-line filter and secure with hose
clamp; connect tubing to in-line filter hose barb on
its other side and secure with hose clamp.
4. 314 series incubators, connect tubing with in-line
filter in place to the appropriate gas inlet fitting on
the back of the unit.
5. Set the supply regulators at 4 psi.
6. Check all of the connections for leaks.
7. Turn the unit power switch to the ON position.
UNPACKING AND INSTALLATION
Warning
While the addition of a fungacide to
the water will assist in controlling
contaminant, it may also affect the
results of experiments conducted
with cells, viruses and other materi-
als. These factors should be taken
into account when deciding whether
or not to use a fungicide.
Warning
High concentrations of carbon diox-
ide product metabolic abnormalities,
disturbances of the central nervous
system and cardiac instability.
Unconsciousness may occur at con-
centrations above 10%.

8
Back of unit
1/4” (0.64 cm) hose
barb connections
(CO2 fitting)
Pressure regulators and gauges must be
installed on each tank. Check local gas supplier
for proper regulators.
Set pressure at 4 psi.
In-line filter
Hose clamp
Hose clamp
1/4” (0.64 cm)
ID tubing
Hose clamp
100% CO2
UNPACKING AND INSTALLATION
Figure A: Back of Unit – Rear Panel View, Exterior

Standard Type (Correct) Siphon Type (Incorrect)
1
2
3
12
3
4
5
6
7
8
1. Compressed Gas Association #320
connector.
2. Gas head space.
3. Liquid CO2 filled to 68% equal weight
of water that cylinder would hold at
60ºF.
1. Warning tag indicating that cylinder is siphoning type.
2* Aluminum ring.
3* Gold band.
4* Stamp or marking on cylinder: "Siphon" or "Eductor
Tube".
5* Valve should be of special type for service.
6* Withdrawal tube draws up the liquid CO2.
7* Gas head-space.
8* Liquid CO2 filled to 68% equal weight of water that
cylinder will hold at 60ºF.
*Note: Some gas suppliers will have no markings to indicate an
“Educator Tube” or “Siphon” type cylinder. Be sure to order
DRY, LAB-GRADE CO2.
Be sure to obtain a dual-stage regulator from the gas supplier
for the CO2 tank that is to be installed according to local
codes. Connect 1/4" ID (6.35 mm) flexible tubing to the regula-
tor. Connect the other end of the tubing to the unit's CO2 inlet
(located on the back top panel). Insert tubing over hose barb
and fasten with an appropriate clamp to assure a proper con-
nection. Adjust the regulator for the pressure recommended
on the previous pages when CO2 is to be injected into cham-
ber. For optimum results, do not exceed or reduce this pres-
sure.
9
UNPACKING AND INSTALLATION

10
Description and Features
1. Control panel
2. CO2 sensor
3. CO2 inlet port
4. Temperature sensor
5. Chamber gasket
6. Chamber shelves
7. Sample port inlet
8. Leveling feet
9. Inner glass door, handle shaft
10. Inner glass door
11. Inner glass door, handle
12. Outer door gasket
13. CO2 sample port
14. Fan Cover
15. Humidity Trough
910 11 12
15
8
7
6
5
4
3
2
1
13
14

11
Chamber Ceiling, Interior
DESCRIPTION AND FEATURES
Circulating Fan
1. Gas Inlet Port
2. Cordset
3. Circuit Breaker(s)
4. RS232 Data Pins Connector
5. Power Switch
4
Rear Panel View, Exterior
5
1
2
3

12
Description
CO2 levels are automatically set at 5%. These units control
CO2 concentrations, as well as temperature, to create a
growth environment ideal for tissue cultures, plants, cells,
viruses and related organisms.
Incoming 100%, CO2, from an external source provided by
the user, passes into the gas inlet port located on the rear
panel of the unit. Heated air is moved uniformly throughout
the chamber by a circulating fan located in the top of the
chamber. A CO2 access port on the control panel permits
sampling of the chamber atmosphere.
These incubators provide precise and reliable control of car-
bon dioxide through the use of a TC, thermal conductivity,
sensor coupled with an easy-to-use command control con-
sole. All required operating commands are immediately
accessible in a functional format on the front of the unit and
permit rapid start-up, close monitoring and efficient control of
incubation protocols.
Temperature is measured by a platinum RTD, resistance tem-
perature detector, producing excellent control within the
range, ±0.1ºC. This is combined with the exclusive
SmartGas®algorithm method for controlling CO2 within the
range ±0.1%. The result is a chamber that produces excellent
environmental conditions for the most sensitive of incubations.
Audible and visual alarms provide a signal to the user of any
deviation in the chamber temperature of ±1.0ºC, or carbon
dioxide of ±1.0% CO2. In addition, other signals alert the
user to power failures and door openings.
An independent, adjustable hydraulic high limit thermostat will
limit the temperature in the event of a problem with the main
temperature controller.
To conserve gas, the unit’s CO2 solenoid valve, as well as the
circulating fan motor, shuts off automatically any time the door
is opened.
A double-door system has a tempered glass inner door that
provides unobstructed viewing of the entire chamber.
314 series chambers are constructed of seamless stainless
steel for cleaning ease and maintenance; 2 stainless steel
shelves are standard—a total capacity is 5 shelves.
Models have a stainless steel outer cabinet with a powder-
coated finish for easy cleaning.
DESCRIPTION AND FEATURES

13
Features
Double-Door Construction with Inner
Door Defogger
A tempered glass inner door allows observation of a
chamber without disturbing the chamber environment.
Inside the steel outer door are heaters that can be turned
on to defog the glass door when necessary. Door seals
to a one-piece gasket for a leak-tight environment.
Control Panel
314 series control panels have a series of command keys
that enable users to enter set point values for tempera-
ture, carbon dioxide, door heat and to calibrate a unit as
required. LED readout provides users with current status
information regarding the foregoing parameters.
DESCRIPTION AND FEATURES
°C
SET CAL CO2 TEMP DOOR
HEAT
SCAN
HOLD
%CO
2
HI LIMIT SAMPLE
121110987654321
1. LED READOUT: The LED displays current CO2
tension and temperature in the Incubator and, in
the set mode, displays the set point values for
these two parameters as well as door heat set-
ting. It also enables the user to enter values
necessary to calibrate temperature and CO2.
Indicating lights and legends serve to identify
which parameter is being displayed.
2. SET COMMAND KEY: The SET key is the
beginning point for and initiates subsequent
actions of the other keys on the control panel.

3. CAL COMMAND KEY: The CAL key permits cal-
ibration of temperature and CO2 when in this
command mode. The Up and Down arrow keys
are used to change the readout to correspond
with the readings obtained from known stan-
dards of temperature or CO2 measurement.
Both temperature and CO2 calibration use the
zero-point shift method.
4. CO2 COMMAND KEY: Pressing the SET key
and then the CO2 key allows the user to enter
the desired carbon dioxide level in the Incubator
with the Up and Down keys .
• High and low limit alarm set points are factory
set at ±1% above and below the user-estab-
lished set point. In the event CO2 exceeds the
indicated limits, an audible alarm will sound and
indicating light above the key will flash.
• Pressing any key will silence the alarm for a
period of one hour. The user should immediate-
ly investigate the reason for the alarm and take
steps to correct the problem. Note that the indi-
cating light continues to flash. If a problem is
not solved the alarm resumes.
• By pressing the CAL (#3) and CO2 (#4) keys
sequentially users can calibrate CO2. The Up
and Down arrow keys will change the readout to
correspond with the readings obtained from
known standards of CO2 measurement.
5. TEMP COMMAND KEY: Pressing the SET key
and then the TEMP command key allows the
user to establish the desired set point tempera-
ture in the Incubator with the Up and Down
arrow keys.
• High and low limit alarm set points are factory-
set at ±1ºC. If the temperature deviates from
these limits, an audible alarm (continuous beep)
will sound.
• While pressing any key will silence the alarm for
a one-hour period, the user should immediately
investigate the reason for the alarm and take
14
DESCRIPTION AND FEATURES

steps to correct the problem. Note that indicat-
ing light continues to flash. If problem is not cor-
rected, the alarm will resume after one hour has
passed.
• By pressing the CAL (#3) and TEMP (#5) keys
sequentially users can calibrate temperature.
The Up and Down arrow keys will change the
readout to correspond with the readings
obtained from known standards of temperature
measurement.
6. DOOR HEAT COMMAND KEY:
•When the SET key is pressed and then the
DOOR-HEAT key, enter the level of heat with the
Up and Down keys as a percentage value being
applied to the inner glass door.
• A flashing DOOR-HEAT indicator light above the
key indicates that heat is being applied to the
inner glass door.
• A good starting point for the door heat control is
35%; if fogging still occurs, increase in 10%
increments until fogging disappears.
7. SCAN/HOLD COMMAND KEY: Permits holding
the value of a single parameter for continued
viewing or scans alternately between the values
of the two parameters on a 2-second interval.
8. DOWN ARROW KEY: This key decreases
numerical value of the parameter that has been
accessed.
9. UP ARROW KEY: This key increases the numer-
ical value of the accessed parameter.
10. HIGH LIMIT THERMOSTAT: This is a backup
high limit temperature thermostat which func-
tions should the microprocessor fail. The high
limit thermostat will then control the temperature
at slightly above the set point.
11. HIGH LIMIT STATUS LAMP: Indicates when the
high limit is active.
15
Note
Decreases in temperature and CO2
due to normal door openings are tol-
erated without initiating the alarms.
DESCRIPTION AND FEATURES
Note
A 5 second delay after any final
entry results in automatic entry of
that change.

12. SAMPLE PORT: This access port is available for
sampling the atmosphere in the chamber when
executing calibration procedure such as might
be done using a Fyrite or other CO2 analysis
procedure.
Proper Sequencing Important in
Operating Command Keys
To enter commands correctly, it is important to press the
keys in SEQUENCE. Do not hold down one key and
press another key—the action will not be accepted by the
system. The following is the proper sequence in which to
press the keys for the indicated actions:
SET(#1), TEMP(#4): View existing value, retain
or change set point using Up and Down keys.
SET(#1), CO2 (#3): View existing value, retain
or change set point using Up and Down keys.
SET(#1), DOOR HEAT(#5): View existing value,
retain or change set point using Up and Down
keys.
CAL(#2), TEMP(#4): Calibrate temperature
using Up and Down keys.
CAL(#2), CO2 (#3): Calibrate carbon dioxide
using Up and Down keys.
CO2 (#3), CAL(#2), SET(#1): Install password
using Up and Down keys.
TEMP(#4), CAL(#2), SET(#1): Disable password
using Up and Down keys
SCAN/HOLD(#6): Each individual contact of key
advances to one of 3 options: A) continuous
viewing of temperature; B) continuous viewing of
carbon dioxide; C) an alternating viewing of the
two.
16
DESCRIPTION AND FEATURES

17
Alarms
POWER OUTAGE: In the event of a power outage, the
LED display will flash to alert the user to take corrective
action. If the power outage is of a temporary nature,
pressing any key will restore unit to operating status.
Everything operates normally after a power outage.
DOOR AJAR WARNING: In case the door is inadvertent-
ly left open beyond a 5-minute period, an audible beep is
sounded and is synchronized with the flashing of the
DOOR-HEAT indicator light to warn the user to correct the
condition.
AUDIBLE AND VISUAL ALARMS: The following table
summarizes the audible and visual alarms that are initiat-
ed upon the occurrence of various events.
Press any key to silence alarm that will remain silent for a
period of one hour. However, the flashing light will contin-
ue. If the condition initiating the alarm remains uncorrect-
ed at the end of that time, the audible alarm will resume.
Password
In the event that you want to prevent unauthorized access
to the operation of the unit, a password feature is avail-
able. See Operation section of this manual.
DESCRIPTION AND FEATURES
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Additional Features
• CO2 sensor (on thermal-conductivity units only).
• Mechanically convected airflow.
• 1.2 cubic feet, 304-stainless steel chamber; full-
view heated glass door
• 2 shelves
18
DESCRIPTION AND FEATURES

19
Operation
Startup and Operation
1. Connect unit to power supply meeting the require-
ments as noted on the nameplate.
2. Turn the power switch ON.
3. Press the SET key first and then the parameter—
CO2, TEMP, DOOR HEAT—which is to be adjusted.
4. Use the UP and DOWN arrow keys to enter the
desired value.
5. Wait 5 seconds and the value is entered automati-
cally into the unit's nonvolatile memory. Storage is
confirmed with two short beeps at the end of 5 sec-
onds.
6. If you access a parameter and initiate no action
within the time frame of 10 seconds, the unit times
out and returns to the prior state.
7. Each press of the SCAN/HOLD key is programmed
to follow a sequential pattern as follows: HOLD
Temperature (for continuous viewing), HOLD CO2
(for continuous viewing) and alternate scanning of
temperature and CO2.
8. To view your previously established set point for any
parameter, press SET and the TEMP, CO2 or
DOOR HEAT key(s).
Password Feature
To prevent unauthorized entry to values that have been set, a
password feature is available with your unit.
1. SET, CAL, CO2 and TEMP keys have the numbers
1, 2, 3 and 4 below that are used to enter or disable
a password. Select a 3-digit password from 001
through 999 (note: 000 is not a valid password).
After power up and after pressing any key, press 3,
2 and 1 in sequence (i.e. CO2, CAL and SET);
enter your numerical password by using the Up or
Down arrow key.
Warning
Do not use in the presence of flam-
mable or combustible materials or
explosive gases. Do not use in the
presence of pressurized or sealed
containers – fire or explosion may
result, causing death or severe
injury.
Warning
Do not heat any substance above a
temperature that will cause it to emit
toxic fumes; death or severe injury
may result.
Warning
Use only carbon dioxide in the incu-
bator. Do not under any circum-
stance inject oxygen or other explo-
sive gas or mixture into unit. Failure
to observe these precautions can
result in explosion and/or fire and
serious injury or death and property
damage.

2. Once your password is entered, wait 5 seconds
and the unit will store this value in its nonvolatile
memory. REMEMBER TO NOTE THE PASS-
WORD IN A PLACE FOR SAFEKEEPING AND
ACCESSIBLE TO YOURSELF OR A COL-
LEAGUE.
3. Subsequent access is denied unless the correct
password is entered. Any keystroke will display
00 and YOU HAVE 5 SECONDS within which to
enter the correct password using the Up or
Down arrow key.
4. To disable the password: enter the password,
wait for . – . – .–. to appear on the readout,
enter 4, 2, 1 (i.e. TEMP, CAL and SET) and the
password is disabled.
Setting Operating Temperature
and High Limit Setpoint
The unit is factory pre-set at 37ºC and 5.0% CO2. To
change these settings:
1. Press the SET and TEMP keys sequentially and
using the Up and Down arrow keys, select the
set point temperature.
2. Using a jeweler’s screwdriver, rotate the high
limit thermostat fully clockwise.
3. Set CO2 set point to zero—accuracy will not be
achieved until the temperature has stabilized.
• Press the SET and CO2 keys sequentially and
using the Up and Down arrow keys, adjust LED
readout until a value of 00.0 is obtained.
• Wait 5 seconds to finalize the selection.
4. Allow sufficient time for the unit to reach and
stabilize at the set point temperature, plus an
additional hour or two for unit to cycle at the
temperature — 4 HOUR TOTAL FOR SETTING
37ºC IS TYPICAL.
20
OPERATION