Thermo Scientific 2862 Operating instructions

Model 2862/2863 (253)
Coliform Incubator Bath
Operating and Maintenance Manual 3177876 Rev. M
Visit us online to register your warranty
www.thermoscientific.com/warranty

Thermo Scientific
MANUAL NUMBER 3177876
M 30674/BA-828 3/14 Revert to Rev K ccs
L 30674/BA-828 1/14 Corrrected Model 2863 line cord, added receptacle in parts list pg 9-1 ccs
K 25227/BA-667 5/09 pg 24 - half coupler
J 25448/BA-670 4/09 UL information
I 24808 9/08 Revised water specs
H 23443/23496 6/06 Consolidated w/ 3177688, removed CE symbol and declaration
G 23196/BA-612 4/06 Modified drain assembly (connection to H2O regulator
F 4/05 New manual #, manufacture location (was 31600111)
E 3/03 Update performance specs, add chamber dimensions - pg 2
D 4/02 Add inlet screen info, correct wire color - pgs 5, 8, 27
Preface
Coliform Incubator Bath i
Models covered by this manual
Catalog No. Volts Hertz Watts Amps Mains Fuse Power Board Fuse
3166678 120 50/60 1550 12.9 N/A N/A
3166679 240 50/60 1550 6.5 FST-5x20-10A FST-6.3x32-6.3mA

Thermo Scientificii Coliform Incubator Bath
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Note The 240V units described in this manual were designed specifically for the European market and are
supplied with a European style power cord. For domestic use, a U.S. style power cord (P/N 3176836) must be
ordered separately. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©1999 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Coliform Incubator Bath iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Coliform Incubator Bath
Preface
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Our staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
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When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
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Coliform Incubator Bath vThermo Scientific
Table of Contents
Introduction and Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Performance Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Unpacking and Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Water Level Regulator (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Inlet Screen Assembly (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Explanation of Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Parts Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Spare Parts and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Assembly Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-3
Electrical Schematics and Wiring . . . . . . . . . . . . . . . . . . . . . . . . . . .9-11
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 8
Section 7
Section 9
Section 10

Coliform Incubator Bath 1-1Thermo Scientific
General Information
Section 1 Introduction and Unpacking
Your satisfaction and safety are important to Thermo and a complete
understanding of this unit is necessary to attain these objectives.
As the user of this apparatus, you have the responsibility to understand the
proper function and operational characteristics of your bath. This
operating manual should be thoroughly read and all operators given
adequate training before attempting to place this unit in service.
Awareness of the stated cautions and warnings, and compliance with
recommended operating parameters — together with maintenance
requirements — are important for safe and satisfactory operation. The
unit should be used as described and for its intended application;
alterations or modifications will VOID THE WARRANTY.
Warning As a routine laboratory precaution, always wear safety glasses
when working with this apparatus.
This product is not intended, nor can it be used, as a sterile or patient
connected device. In addition, this apparatus is not designed for use in
Class I, II or III locations as defined by the National Electrical Code. s
Caution When unpacking this product, two persons are required to lift the
water bath and place it on a bench.
The bench top must be rigid and strong enough to comfortably support
the weight of the unit when filled with water. s
Precision Circulating Baths are widely used in research and quality control.
Their superb temperature uniformity and stability makes them especially
desirable for legal or reference tests.
The microprocessor control panel houses all functions necessary to operate
the bath. The 4 push-button switches and single digital display window
allow the operator to adjust bath temperature and temperature calibration
via a single set of controls.
The proportional integral temperature control coupled with agitation
supplied by a centrifugal pump located in the control housing allows
precise temperature control. Use of the gable cover provided is required to
maintain optimal temperature sensitivity.

1-2 Coliform Incubator Bath Thermo Scientific
Section 1
Introduction and Unpacking
A high limit thermostat is provided and can be set to prevent heater
runaway in the event of temperature control failure.
The interior of the bath is constructed of stainless steel and is designed for
operation with distilled water or water solutions, such as water /ethylene
glycol with corrosion inhabitants added. The body is made from
galvanized steel and is painted for added protection. A drain is located at
the far left hand end of the bath. A stainless steel gable cover is also
provided with the bath.
The 240-volt units are identical in appearance to the 120-volt units.
The following table identifies the specifications for the Coliform Baths.
Technical Specifications
Temperature Settings (factory preset)
35.0°C 41.5°C
44.5°C 45.5°C
Sensitivity (cover on) . . . . . . . . . . . . . . . . . .±0.05°C
Uniformity (cover on) . . . . . . . . . . . . . . . . . .±0.05°C
Chamber Size
Length . . . . . . . . . . . . . . . . . . .27 inches (68.6cm.)
Width . . . . . . . . . . . . . . . . . . . .12 inches (30 cm.)
Height . . . . . . . . . . . . . . . . . . 7.5 inches (19 cm.)
Capacity (1.5" from top) . . . . . . .9.1 gallons (34.5L.)
General Information
(continued)
Performance Data

Coliform Incubator Bath 1-3Thermo Scientific
Section 1
Section title
Save all packing material until unit is put into service. This merchandise
was carefully packed and thoroughly inspected before leaving our factory.
Responsibility for safe delivery was assumed by the carrier upon acceptance
of the shipment; therefore, claims for loss or damage sustained in transit
must be made upon the carrier by the recipient as follows:
•Visible Loss or Damage: Note any external evidence of loss or damage
on the freight bill, or express receipt, and have it signed by the carrier's
agent. Failure to adequately describe such external evidence of loss or
damage may result in the carrier's refusing to honor your damage
claim. The form required to file such claim will be supplied by the
carrier.
•Concealed Loss or Damage: Concealed loss or damage means loss or
damage which does not become apparent until the merchandise has
been unpacked and inspected. Should either occur, make a written
request for inspection by carrier's agent within fifteen (15) days of the
delivery date; then file a claim with the carrier since the damage is the
carrier's responsibility.
If you follow the above instructions carefully, we will guarantee our full
support of your claim to be compensated for loss or concealed damage.
DO NOT — FOR ANY REASON — RETURN THIS UNIT
WITHOUT FIRST OBTAINING AUTHORIZATION. In any
correspondence to Thermo, supply the nameplate data, including catalog
number and serial number.
Unpacking and
Damage

Coliform Incubator Bath 2-1Thermo Scientific
Section 2 Installation
Warning Installation should be completed by qualified personnel only. s
Location - The most uniform operating conditions will be obtained by
placing the bath on a level surface in an area remote from drafts,
ventilating outlets, radiators, and other rapidly changing ambient
conditions.
Environmental Conditions- This instrument is designed to operate safely
under the following conditions:
• Indoor Use Only
• Temperature: 5° to 40°C
• Maximum Relative Humidity: 80% for temperatures to 22°C
• Maximum Altitude 2000 meters
• Mains Supply Voltage Fluctuation ±10, or per project plan
• Installation (Overvoltage) Category II, Pollution Degree 2
Maximum performance is assured across the 15°C to 45°C temperature
range.
1 Installation category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which is
the category used for instruments in installations supplied from a supply comparable to
public mains such as hospital and research laboratories and most industrial laboratories,
the expected transient overvoltage is 2500V for a 230V supply and 1500V for
a 120V supply.
2 Pollution Degree describes the amount of conductive pollution present in the operating
environment. Pollution Degree 2 assumes that normally only non-conductive pollution
such as dust occurs with the exception of occasional conductivity caused by condensation.

2-2 Coliform Incubator Bath Thermo Scientific
Section 2
Installation
Electrical Connections -
Warning For personal safety, this apparatus must be properly grounded. s
1. The power cord provided on this unit is equipped with a three-prong
(grounding) plug which mates with a standard three-prong grounding
wall receptacle to minimize the possibility of electric shock hazard
from this apparatus. If in doubt, the user should have the wall
receptacle and circuit checked by a qualified electrician to make sure
the receptacle provides adequate current and is properly grounded.
2. Where a standard two-prong wall receptacle is encountered, it is the
personal responsibility and obligation of the user to have it replaced
with a properly grounded three-prong wall receptacle. Do not, under
any circumstances, cut or remove the third (ground) prong from the
power cord. Do not use a two-prong adapter plug.
Determine the total amount of current being used by other apparatus
connected to the circuit to be used for this apparatus. It is critical that the
added current demand of this (see nameplate) and other equipment used
on the same circuit does not exceed the rating of the fuse or circuit
breaker.
Caution Be sure that the power supply is the same voltage as on the
nameplate.
The power cord is the mains disconnect. Make sure the receptacle is always
easily accessible.
To avoid build-up of mineral deposits and to prevent corrosion, use only
distilled water (50K ohm to 1M ohm) in the bath. Using chlorinated tap
water or additives containing chlorine will void the manufacturer's
warranty. Similarly, high purity (deionized) water that does not fall within
a 50K to 1M ohm resistivity range and a 7 to 9 pH range will also void
the warranty. Contact Technical Services with any questions.
Electrolysis can damage stainless steel if an object is allowed to rest directly
on the surface, trapping moisture that becomes oxygen starved but is
surrounded by water containing oxygen. The resulting electrolytic action
will pit or erode the stainless steel. s

Coliform Incubator Bath 2-3Thermo Scientific
Section 2
Installation
Water Level
Regulator (Optional)
Use the following steps with Figures 2-1 and 2-2 shown on the following
page.
1. Turn off unit, disconnect power and drain water from the bath.
2. Unscrew drain plug from side of bath body.
3. Replace with elbow connector and reducing bushing (shipped loose).
Note the reducing bushing is used on units built after February 2006.
Note To ensure a good seal, wrap threads with Teflon tape or equivalent
sealer. s
4. Tighten elbow connector so open end (tube fitting) faces straight
down.
5. Place Water Level Regulator Bracket Assembly on the lip of the bath
pan (drain side of the bath towards the rear of the unit). The small
flange with gasketing near the top of the bracket should rest against the
inside of the bath pan. The other 2 flanges with gasketing should rest
against the outside of bath body.
6. Insert the plastic tubing (coming from the bottom of the water level
regulator) into the elbow connector (at the drain) and tighten the
elbow connector fitting firmly to ensure a good seal.
7. Uncoil Tygon tubing and press onto 1/4" Hose Barb Fitting (located at
the top of water level regulator). Hose clamps are provided to ensure a
good seal.
8. Hook-up other end of the tygon tubing to water supply and turn on
water. Water pressure should fall within the following range: MIN is
approximately 15 PSI, MAX is approx 75 PSI.
Note Water line marking on the water level regulator is an indicator od the
approximate water level that is to be maintained in the bath. The assembly
has been set to maintain approximately 1¾” of water. To raise the set water
level, loosen the thumbscrew and raise the water level regulator to the
sdesired level (coincide with water line mark) and tighten thumbscrew to
hold water level regulator in place. s
Note Do not overtighten the thumbscrew. s

2-4 Coliform Incubator Bath Thermo Scientific
Section 2
Installation
To Water
Supply
Vent Tube
Bracket
Plastic Tubing
Bath Drain
Elbow Connector
Tygon Tubing
1/4” Hose Barb
Fitting
Water Level
Regulator
Reducing Bushing
To Water
Supply
Thumbscrew
Bath Body
Bath Pan
Gasket
Fitting
Figure 2-1. Side View
Figure 2-2. Cutaway Front View

Coliform Incubator Bath 2-5Thermo Scientific
Section 2
Installation
SILICON HOSE
WATERBATH PAN
NOTE: INLET IS THE LOWER
OF THE TWO PORTS
DIRECTION OF WATER FLOW
SCREEN ASSEMBLY
(FINGER TIGHTEN ONLY)
TUBE ADAPTER
INLET NUT
Figure 2-3. Inlet Screen Assembly
An optional inlet screen assembly is packaged with each unit. If used, this
screen will filter foreign materials (i.e. pieces of paper) from being sucked
through the pump. To install the screen assembly, refer to the diagram
below.
Note If used, the screen assembly must be inspected daily. Remove any
debris found on the screen. s
Inlet Screen
Assembly (Optional)

Coliform Incubator Bath 3-1Thermo Scientific
Section 3 Explanation of Controls
Power Switch - The power switch is located on the lower right hand side
of the bath, it provides power for the entire unit.
Hi-Limit Thermostat - The hi-limit thermostat is located immediately to
the left of the power switch on the lower right hand corner of the bath.
The high limit is an adjustable control used to prevent thermal runaway
in the event that the primary control fails. After the bath temperature is
stabilized, use a standard screwdriver to adjust the potentiometer. When
delivered, the Hi-Limit is turned fully clockwise (highest setting). In this
position, the Hi-limit light will be OFF. Turn the potentiometer coun-
terclockwise until the Hi-Limit light is ON, thenadjust clockwise 1/8 of
a turn. Verify that the Hi-Limit light is OFF. Now if the bath tempera-
ture rises above the desired setting, the Hi-Limit protection will be acti-
vated.
Control Panel - The control panel is located on top of the bath and pro-
vides primary temperature control. The control panel contains the fol-
lowing features (See Figure 3-1).

3-2 Coliform Incubator Bath Thermo Scientific
Section 3
Explanation of Controls
1. LED Display - The three-digit LED display is used to show both the
actual and set point values for the temperature.
2. Heater On Lamp - The "Heater ON" lamp is illuminated when power
is applied to the heater.
3. Calibrate - The Calibrate key is used to adjust the actual temperature
value to match a calibrated reference thermometer.
4. Temperature Selection - ("UP" or "Down") These keys are used to
select one of the four temperature setpoints.
5. Enter - The Enter key is used to store a new setpoint value.
Allow to stabilize.
calibration is complete.
q
Press or to match readout
Press Calibrate button.
p
to thermometer.
Press ENTER
Place certified / traceable
Display will then return
New Set Point is accepted.
desired temperature is displayed.
Are Factory Preset
thermometer into holder.
Should Calibration
Press or until
to actual temperature.
To Select Temperature:
Selected Temperatures
Calibrate
ENTER
Heater On
Be Required:
Press ENTER
pq
Figure 3-1. Controls
LED Display
Heater ON
Lamp
Calibrate
Key
Temp Selection
Keys
Enter Key

Coliform Incubator Bath 4-1Thermo Scientific
Section 4 Operation
Caution Exercise care when using acidic or caustic solutions as they will
attack the galvanized steel bath body if spilled into the bath. If spills do
occur, the bath liquid should be immediately drained and the unit
thoroughly flushed. Spills and condensation should be cleaned/removed
from all metal surfaces after each use. s
When filling the bath with water, allowance must be made for the
displacement of water upon immersion of samples. Do not use deionized
water.
Caution To avoid build-up of mineral deposits and to prevent corrosion,
use only distilled water (50K ohm to 1M ohm) in the bath. Using
chlorinated tap water or additives containing chlorine will void the
manufacturer's warranty. Similarly, high purity (deionized) water that does
not fall within a 50K to 1M ohm resistivity range and a 7 to 9 pH range
will also void the warranty. Contact Technical Services with any questions.
Electrolysis can damage stainless steel if an object is allowed to rest directly
on the surface, trapping moisture that becomes oxygen starved but is
surrounded by water containing oxygen. The resulting electrolytic action
will pit or erode the stainless steel. s
Caution Minimum water level requirements - water level should be 1½
inches above the pump outlet. This will keep the pump from running dry
and failing to operate. Maximum water level should not be higher than 1
inch from the top of the bath pan. s
To conserve energy, reduce evaporation, and increase temperature control
accuracy, use the gable cover provided. Do not use aluminum foil as a
cover, as it may cause corrosion due to an electrochemical reaction.