Thermo Scientific BB 150 User manual

CO2Incubator BB 150 Operating Instructions
2
Copyright©
These operating instructions are protected by copyright. Rights resulting thereof,
particularly reprint, photomechanical or digital postprocessing or reproduction, even
in part, are only allowed with the written consent of Thermo Fisher Scientific.
This regulation does not apply to reproductions for in-plant use.
The contents of the operating instructions are subject to change without further
notice. For translations into foreign languages, the German version of these ope-
rating instructions is binding.
Trademarks
All trademarks mentioned in the operating instructions are the exclusive property
of the respective manufacturers.
Thermo Fisher Scientific

CO2Incubator BB 150 Operating Instructions 3
Contents
1. General notes ........................................................................................... 6
1.1 General safety instructions ............................................................ 7
1.2 Warranty ........................................................................................ 7
1.3 Explanation of symbols .................................................................. 8
1.3.1 Symbols used in the operating instructions ...................................8
1.3.2 Symbols on the device ................................................................... 9
1.4 Intended purpose of the device....................................................10
1.4.1 Correct use .................................................................................. 10
1.4.2 Incorrect use ................................................................................ 10
1.5 Standards and directives..............................................................10
1.6 Safety notes on gases ................................................................. 11
2. Delivery ................................................................................................... 12
2.1 Packaging .................................................................................... 12
2.2 Components standard equipment ................................................12
2.3 Acceptance inspection .................................................................12
3. Installation of the device ....................................................................... 13
3.1 Ambient conditions.......................................................................13
3.2 Room ventilation .......................................................................... 13
3.3 Space requirements .....................................................................14
3.4 Transport ......................................................................................14
3.5 Stacking ....................................................................................... 15
3.6 Modifications ................................................................................ 15
4. Description of the device ...................................................................... 16
4.1 Front view .................................................................................... 16
4.2 Rear view ..................................................................................... 17
4.3 Safety devices.............................................................................. 18
4.4 Work space atmosphere ............................................................... 18
4.5 ContraCon decontamination routine .............................................. 19
4.6 Sensor system ............................................................................. 19
4.7 Door switch .................................................................................. 20
4.8 Switchbox with supply interface .................................................... 20
4.9 Work space components .............................................................. 21
5. Start-up .................................................................................................... 23
5.1 Preparing the work space ............................................................. 23
5.2 Mounting the door handle .............................................................. 23
5.3 Installing the shelf system ............................................................ 24
5.4 Gas connection ............................................................................ 26
5.5 Power supply connection .............................................................. 27
5.6 RS 232 interface connection ......................................................... 28
5.7 Connecting the alarm contact ....................................................... 30
6. Handling and control ............................................................................. 32
6.1 Power switch ................................................................................ 32
6.2 Operating panel ............................................................................ 32
6.3 Control self-test ............................................................................ 33
6.4 Setting the nominal temperature value .......................................... 34
6.5 Setting the CO2nominal value ...................................................... 34
6.6 Activating the stacked incubator mode ......................................... 35
6.7 Activating the auto-start routine .................................................... 36

CO2Incubator BB 150 Operating Instructions
4
Contents
6.8 Reading failure codes ................................................................... 38
6.9 Failure code list ............................................................................ 41
6.10 Resetting the thermal protection ................................................... 42
7. Operation ................................................................................................ 43
7.1 Preparing the device ..................................................................... 43
7.2 Starting operation .......................................................................... 43
8. Shut-down ............................................................................................... 45
8.1 Shutting the device down .............................................................. 45
9. Cleaning and disinfection ..................................................................... 46
9.1 Decontamimation procedures ....................................................... 46
9.2 Wipe/Spray disinfection ................................................................ 46
9.3 ContraCon decontamination routine .............................................. 50
9.4 Activating the ContraCon decontamination routine ........................ 52
9.5 Cancelling the ContraCon decontamination routine ....................... 52
10. Maintenance ........................................................................................... 53
10.1 Inspections and checks ................................................................ 53
10.2 Service intervals ............................................................................ 53
10.3 Preparing the temperature calibration ........................................... 54
10.4 Temperature calibration procedure ................................................ 55
10.5 Preparing the CO2calibration ........................................................ 56
10.6 CO2calibration procedure ............................................................. 57
10.7 Replacing the sterile filters ........................................................... 58
10.8 Replacing the device fuses ........................................................... 58
10.9 Replacing the door seal ................................................................ 59
11. Spare parts and accessories ................................................................. 60
11.1 List of spare parts and accessories .............................................. 60
12. Technical data ........................................................................................ 62
13. Disposal ................................................................................................... 65
14. Principles of good microbiological practice ....................................... 67
15. Device log ............................................................................................... 69
16. Certificate of decontamination ............................................................. 70

CO2Incubator BB 150 Operating Instructions 5
Figures
Fig. 1 Device dimensions and clearance ............................................... 14
Fig. 2 Lift points ......................................................................................14
Fig. 3 Stacking ....................................................................................... 15
Fig. 4 Front view .................................................................................... 16
Fig. 5 Rear view ..................................................................................... 18
Fig. 6 Temperature and CO2sensor ......................................................19
Fig. 7 Door switch .................................................................................. 19
Fig. 8 Supply interfaces ......................................................................... 20
Fig. 9 Work space layout, Water reservoir .............................................20
Fig.10 Rear panel openings ....................................................................22
Fig.11 Door handle ................................................................................. 23
Fig.12 Support rail installation/removal ...................................................24
Fig.13 Center strut/gas humidification installation ..................................25
Fig.14 Gas pressure hose installation..................................................... 26
Fig.15 Power supply connection .............................................................27
Fig.16 RS 232 interface connection ........................................................28
Fig.17 Example of a connection scheme for an external alarm system . 31
Fig.18 Power switch ................................................................................ 32
Fig.19 Operating panel functions ............................................................. 32
Fig.20 Water pump .................................................................................. 48
Fig.21 Removing blower wheel and cover ................................................. 49
Fig.22 ContraCon decontamination routine procedure ............................. 51
Fig.23 Temperature calibration ................................................................. 54
Fig.24 CO2calibration .............................................................................. 56
Fig.25 Sterile filter replacement ............................................................... 58
Fig.26 Device fuse replacement ............................................................... 58
Fig.27 Door seal replacement .................................................................. 59
Fig.28 Gas consumption ......................................................................... 64

CO2Incubator BB 150 Operating Instructions
6
1. General notes
The following are Thermo Fisher Scientific service contact
information.
For customers in China:
Tel. [phone]
Fax [phone]
Website: http://thermofisher.com
For customers outside of China: Please contact local distributors for technical
support

CO2Incubator BB 150 Operating Instructions 7
1. General notes
1.1 General safety instructions
These operating instructions describe the CO2incubator BB 150.
The CO2incubator has been manufactured in keeping with the latest techno-
logical developments and is operationally safe. However, the device may pre-
sent potential hazards, particularly if it is operated by inadequately trained personnel
or if it is not used in accordance with the intended purpose. Therefore, the following
must be observed to prevent accidents:
• The CO2incubator must only be operated by trained and authorized personnel.
• For personnel operating this device, the operator must prepare written instruc-
tions in a reasonable form based on these operating instructions, the safe-
ty data sheets, the hygiene regulations and the applicable Technical Guidelines,
in particular:
• which decontamination measures are to be taken for the device and
for the accessories used,
• which safety measures are to be taken when gases and pressurized
gas containers are used,
• which measures are to be taken in case of an accident.
• Any repairs to the device must only be performed by adequately trained and
authorized expert personnel.
• The contents of the operating instructions are subject to change without further
notice.
• Concerning translations into foreign languages, the English version of these
operating instructions is binding.
• Keep these operating instructions in the vicinity of the device so that safety in-
structions and important information is always accessible.
• Should you encounter problems that are not mentioned in these operating
instructions, please contact Thermo Fisher Scientific immediately for your own
safety.
1.2 Warranty
Thermo Fisher Scientific Products warrant the operation and the operational safety
of the CO2incubator BB 150 only under the condition that:
• the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Thermo
Fisher Scientific Products are used,
• inspections and maintenance works are carried out at the specified intervals.
The warranty is valid from the date of delivery of the device to the operator.

CO2Incubator BB 150 Operating Instructions
8
1. General notes
1.3 Explanation of symbols
1.3.1 Symbols used in the operating instructions
WARNING!
is used if non-observance may cause serious
or even lethal injuries.
CAUTION!
is used if non-observance may cause medium
to minor injuries or damage.
NOTE
is used for applicational hints and useful
information.
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!

CO2Incubator BB 150 Operating Instructions 9
1. General notes
1.3.2 Symbols on the device
CE symbol
VDE - safety-tested
Test certificate for USA/Canada
Observe operating instructions!

CO2Incubator BB 150 Operating Instructions
10
1. General notes
1.4 Intended purpose of the device
1.4.1 Correct use
The CO2incubator BB 150 is a laboratory device for preparing and cultivating cell
and tissue cultures. The device allows the simulation of the special physiolo-
gical ambient conditions for these cultures due to the exact control of:
• temperature,
•CO
2 content,
• an increased relative humidity.
The BB 150 has been designed for installation and operation in the following fields
of application:
• Laboratories for cytobiological and biotechnological experiments of safety
levels L1, L2, and L3.
• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.
The CO2required for the incubator is supplied to the device from a separate gas
supply system, either from gas cylinders or from a central pressurized gas con-
tainer.
The layout of the gas supply system must ensure that the operating pressure
of the gas supply lines can be set to a range between 0.8 bar (min.) to 1 bar (max.)
and that the pressure cannot be changed.
Depending on the capability of the gas supply system, several devices may be
connected to one gas cylinder.
The CO2incubator is suited for continuous operation.
1.4.2 Incorrect use
Do not use cell or tissue cultures in the device that are not in accordance with
the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitible or explosive,
• release vapors that form combustible or explosive mixtures when exposed
to air,
• release poisons.
1.5 Standards and directives
The device is in accordance with the following standards and guidelines:
• DIN EN 61010
• Low Voltage Guideline 73/23 EWG
• EMC Guideline 89/336 EWG
• UVV VBG 20
• DIN 12880 Part 1/11.78

CO2Incubator BB 150 Operating Instructions 11
1. General notes
The following safety regulations must be observed if the device is operated within
the territory of the Federal Republic of Germany:
• ZH 1/10
• ZH 1/119
• ZH 1/342
• ZH 1/343
• ZH 1/598
• TRG 280
• EC Official Gazette, L 374
• Safety data sheets of the gas supplier relevant to the particular characteri-
stics of CO2.
• Principles of good microbiological proceedings, notice of the trade association
of the German chemical industry.
For other countries, the applicable national regulations are binding.
1.6 Safety notes on gases
Carbon dioxide (CO2):
As CO2is rated as a harmful gas, certain safety instructions must be observed
when the CO2incubator is started up and when the device is operated.
NOTE – Personnel instruction
Personnel operating devices with a CO2sup-
ply must be instructed about the requirements
for the handling of CO2before starting their
work:
• Correct operation of pressurized gas con-
tainers and gas supply systems
(e.g. TRG 280),
• Obligation to report damages and shortco-
mings in CO2supply lines,
• Measures to be taken in case of accidents
or failures.
These instructions must be repeated at appropriate intervals and must incor-
porate the particular operating instructions of the gas supplier.
WARNING - Suffocation hazard!
CO2released in large amounts into the room
atmosphere may cause suffocation.
If CO2is released, initiate safety measures
immediately!
• Leave the room immediately and do not al-
low others to enter the room!
• Inform security service or fire department!

CO2Incubator BB 150 Operating Instructions
12
2. Delivery
2.1 Packaging
The CO2incubator BB 150 is delivered in a stable packaging box. All packaging
materials can be separated and are reusable:
Packaging materials
• Packaging carton Recycled paper
• Foam elements Styrofoam (CFC-free)
• Pallet Untreated wood
• Packaging film Polyethylene
• Packaging ribbons Polypropylene
2.2 Components standard equipment
2.3 Acceptance inspection
After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damages.
If damages are detected or if components are missing, please contact the
carrier and Thermo Fisher Scientific Products immediately.
Delivery
CO2incubator with
single glass door and with
continuous shelves
(standard version)
Shelf 3
Shelf support post 4
Shelf support rail 6
Insert for pressure compensation
opening 1
Plug for pipe channel 1
Power supply cable 1
Connector, potential-free contact 1
Spare caps, set 1
CO2connecting hose set 1
Immersion water pump 1
Open-end wrench,
24 mm 1
Allen wrench 2 mm for blower
wheel 1
Allen wrench 3 mm for blower
wheel cover 1
Operating instructions 1

CO2Incubator BB 150 Operating Instructions 13
3. Installation of the device
3.1 Ambient conditions
The device must only be operated in locations that meet the particular ambient
conditions listed below.
Requirements:
• Draft-free and dry location.
• The minimal distance to adjacent surfaces must be observed on all sides (see
Section 3.3.).
• The operating room must be equipped with appropriate room ventilation.
• Solid, level, fire-proof surface.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the
weight of the device and accessories (particularly if several devices are stak-
ked).
• To ensure a constant incubation temperature of 37° C, the ambient tempe-
rature must be within a range of +18° C to +33° C.
• Relative humidity up to 80 % (max.).
• Avoid direct exposure to sunlight.
• Devices that produce excessive heat should not be used near the location
of the CO2incubator BB 150.
3.2 Room ventilation
When CO2is supplied, the work space of the incubator is slightly pressurized. The
pressure is released through the pressure compensation opening into the operating
room.
As the pressure compensation and any opening of the glass door/gas tight screen
during the operation of the device will release very small quantities of CO2into
the operating room, the room ventilation must be capable of carrying the released
gas safely off into the open.
In addition, heat dissipating from the device during permanent operation may
cause a change in the room climate.
• Therefore, the BB 150 must only be installed in rooms with sufficient ventilation.
• Do not install the device in room recesses without ventilation.
• The room ventilation should be a technical ventilation that is in accordance
with the requirements of ZH 1/119 (Guidelines for laboratories) or some other
suitable ventilation system with appropriate capacity.

CO2Incubator BB 150 Operating Instructions
14
3. Installation of the device
3.3 Space requirements
Fig. 1: When installing the device, make sure that the installa-
tion and supply connections are freely accessible.
The control box at the rear panel of the device may serve as
a spacer to adjacent objects. The side distances given are mi-
nimal distances.
To protect the CO2incubator against contamination, use a floor
stand even if the device is installed near the floor. The height
of the floor stand should not be less than 200 mm.
Several floor stands and carriers are available as options from
Thermo (part numbers see Section 11.1, “Spare parts and
accessories”).
NOTE – Accessibility of the devices
To ensure the accessibility for care and mainte-
nance works, keep larger side and rear
distances.
3.4 Transport
Fig. 2: For transport do not lift the device using the doors or
components attached to the device (e.g. control box on rear
panel) as lift points.
NOTE – Lift points
Lift the device only using the lift points shown
in Fig. 2.
Fig. 1
Device dimensions and
clearance in mm,
F = Front
Fig. 2
Lift points

CO2Incubator BB 150 Operating Instructions 15
3. Installation of the device
3.5 Stacking
Fig. 3: Two BB 150 devices can be stacked on top of each other.
The stacking elements [1] and [2] secure the devices on top
of each other.
If the devices are placed onto mobile racks, ensure that the
rollers [4] are secured by an arresting device during operati-
on. For reasons of stability, the rollers should be oriented to
the front.
When stacking the incubators, the stacked incubator function
must be activated on the upper unit. This function adjusts the
parameters of the uppper unit to compensate any heat trans-
mission between the two units.
NOTE – Transporting stacked devices
The stacking elements are anot connecting
elements. Therefore, the transport of stacked
devices on sloped surfaces is not allowed.
3.6 Modifications
The outer door and the glass door can be equipped with left
or right side fasteners. The door fastening can also be reversed
later.
NOTE – Modifications
Retrofitts and modifications must only be per-
formed by the Technical Service of Thermo
Fisher Scientific Products.
Fig. 3
Stacking

CO2Incubator BB 150 Operating Instructions
16
4.1 Front view
[1] Stacking elements
[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Pressure compensation opening with insert
[7] Measuring opening
[8] Outer door
[9] Door handle
[10] Outer door seal, replaceable
[11] Stand, height-adjustable
[12] Nameplate
[13] Power switch
[14] Support rail
[15] Shelf
[16] Latch, glass door
[17] Support hook for shelf
[18] Access port with plug
[19] Glass door seal, replaceable
4. Description of the device
Fig. 4
Front view

CO2Incubator BB 150 Operating Instructions 17
4.2 Rear view
[1] Stacking elements
[2] Pressure compensation opening
[3] Access port, Ø 42 mm
[4] Switchbox with supply interfaces
[5] CO2gas container
4. Description of the device
Fig. 5
Rear view

CO2Incubator BB 150 Operating Instructions
18
4. Description of the device
4.3 Safety devices
The device is equipped with the following safety devices:
• A door switch interrupts the CO2supply and the work space heating when
the glass door is opened.
• An independent thermal protection protects the samples from harmful over-
heating in the event of a failure.
• A pressure compensation opening ensures pressure compensation in the
device work space.
• Audible and visual alarms indicate failures during operation.
4.4 Work space atmosphere
In the work space of the incubator, the particular physiological ambient condi-
tions for the preparation and cultivation of cell and tissue cultures are simula-
ted. The work space atmosphere is determined by the following factors:
• Temperature,
• Relative humidity,
•CO
2concentration.
Temperature:
To ensure undisturbed operation, the temperature in the operating room must
be at least 18° C and the incubation temperature must be at least 3° C higher
than the room temperature.
The heating system controls the incubation temperature from this temperature
threshold up to 55° C. The principle of air jacket heating and the additional, se-
parate heating of the outer door minimize the risk of condensate formation on
the side walls, at the ceiling of the work space, and at the glass door.
Relative humidity:
The water tray of the work space can hold 3.0 liters of processed water. The hea-
ting of the work space causes evaporation of the water, thereby ensuring a con-
stant humidity within the work space. Under normal operating conditions and
at the usual incubation temperature of 37° C, a constant relative humidity of approx
95 % is achieved in the work space.
If heated containers are removed and put back into the work space, the elevated
humidity and the cooling may cause condensate to form on the outer sides of
the container.
For humidification, processed water of the following quality is required:
• demineralized and either distilled or autoclaved for sterilization,
or
• completely deionized and either distilled or autoclaved for sterilization.
CO2supply:
To ensure correct growth conditions for the cell and tissue cultures, the work space
is supplied with CO2. The pH of the bicarbonate-buffered culture media large-
ly depends on the CO2content of the work space atmosphere. The CO2con-
tent of the work space atmosphere can be controlled within a range of 0-20 %.
The CO2must be of the following quality:
• Purity 99,5 % min.,
or
• medical grade.

CO2Incubator BB 150 Operating Instructions 19
4. Description of the device
4.5 ContraCon decontamination routine
The ContraCon decontamination routine is used to
decontaminate the complete work space including all installed
components and sensors.
During this routine, a moist and wet atmosphere with highly
decontaminating effect is created for 9 hours at a temperature
of 90° C.
The effectiveness of the ContraCon decontamination routine has
been tested and certified by independent institutes. Information
about these tests is available at request from Thermo Electron
LED.
The entire program run of the ContraCon decontamination routine
will take approx 25 hours.
After the run has been completed, the device must be reactivated
using the autostart routine.
NOTE – Thermal protection
If the thermal protection for the device
responds, the ContraCon decontamination
routine can only be be started after the cause
of the failure has been repaired or reset
(see Section 6.13).
4.6 Sensor system
Fig. 6: The blower wheel and two sensor modules are integrated
in the baseplate [1] of the measuring cell:
• Sensor [2] for the measurement of the work space tempe-
rature and of the thermal protection,
•CO
2sensor [3] for the measurement of the CO2content in
the work space atmosphere.
The sensor for the measurement of the work space temperature
as well as the CO2sensor form part of the control system of
the device. Their measured values are compared to the set no-
minal values. Based upon this data, the control system cont-
rols heating and CO2supply.
The blower intermixes the supplied gases and ensure uniform
temperature distribution within the work space.
The thermal protection has been preprogrammed at the fac-
tory and cannot be changed. It protects the stored cultures from
overheating. If the temperature is exceeded by more than
1° C, the thermal protection responds and the work space tem-
perature is automatically reduced to the set nominal value so
that the incubation process can be continued even in case of
a failure. Any response of the thermal protection will simulta-
neously trigger an audible and visual alarm.
Fig. 6
Temperature and CO2
sensor

CO2Incubator BB 150 Operating Instructions
20
4. Description of the device
4.7 Door switch
Fig. 7: A door switch [1] is installed in the upper edge of the work
space opening. If the door switch is activated by opening the
glass door, the gas supply and the heating of the work space are
interrupted and the display shows will flash.
If the door remains open for more than 30 seconds, a short
acoustic alarm sounds. If the door remains open for more than
10 minutes, the acoustic alarm sounds continuously.
The outer door can only be closed after the glass door has been
latched properly.
4.8 Switchbox with supply interface
All supply connections are installed in the switchbox at the rear
of the device.
Gas connection:
Fig. 8: The gas supply line between the device and the gas
supply system is connected using the supplied connecting
hoses. CO2 is supplied to the device through a separate con-
necting sleeve [1].
The process gas must be supplied to the device at a fixed
pressure that has been preset within a range of 0.8-1.0 bar,
this must remain unchanged.
Before the gas is fed into the work space, it flows through a
sterile filter with a separation rate of 99.97 % for a particle size
of 0.3 μm (HEPA filter quality).
Label:
Fig. 8: The label [2] contains information about gas supply, an
alarm contact terminal legend, and notes about the electrical
fusing of the device.
RS 232 interface:
Fig. 8: Via the RS 232 interface [3], the incubator can be con-
nected to the serial interface of a PC. This connection allows
the computer-aided acquisition and documentation of the major
operating parameters (temperature, CO2concentration, failure
codes, etc.).
Alarm contact:
Fig. 8: The device can be connected to an on-site, external
alarm system (e.g. telephone system, building monitoring
system, visual or acoustic alarm system). For this purpose, a
potential-free alarm contact [4] is preinstalled in the device. Fig. 8
Supply interfaces
Fig. 7
Door switch